1. One purpose of culture method is to ISOLATE bacteria in pure culture.

2. Type isolates by methods such as _______. BACTERIOPHAGE

3. BACTERIOCIN SUSCEPTIBILITY
4. A type of culture method which one loopful of culture is made as a primary inoculum and is then
distributed thinly over the plate. STREAK METHOD
5. It provides a uniform growth of a bacterium. Also called a carpet method. LAWN CULTURE
6. This method is employed for providing a pure growth of the bacterium for slide agglutination and other
diagnostic tests. STROKE CULTURE
7. This method is prepared by puncturing with a long straight, charged wire in a suitable medium. STAB
CULTURE
8. The suitable medium could be NUTRIENT GELATIN / GLUCOSE AGAR
9. This method gives an estimate of the viable bacterial count in a suspension. POUR PLATE CULTURE
10. What is the name of the device used in enumerating the number of colonies in pour plate culture. COLONY
COUNTER
11. This method is preferably used when large yields are desired. LIQUID CULTURE
12. CELL STAINING is a technique used for the main purpose of increasing contrast through changing the colour
of some of the parts of the structure being observed.
13. IN-VIVO STAINING - refers to the staining of a biological matter while it is still alive
14. IN-VITRO STAINING - refers to a staining technique where the biological matter is non-living.
15. The actual cells are colored and appeaar in a clear background. POSITIVE STAINING
16. It involves only one stain. You may choose from methylene blue, Gram safranin, and Gram crystal violet.
SIMPLE STAINING
17. It is a staining process which uses more than one chemical stain. DIFFERENTIAL STAINING
18. Gram staining uses two dyes: CRYSTAL VIOLET and
19. FUCHSIN OR SAFRANIN
20. The actual cells remain clear (uncolored) and the background is colored to create a contrast to aid in the
better visualization of the image. NEGATIVE STAINING
21. GRAM STAINING is used to determine gram status to classify bacteria broadly. It is based on the
composition of their cell wall.
22. Gram staining uses CRYSTAL VIOLET to stain cell walls
23. Gram staining uses a FUCHSIN OR SAFRANIN counterstain to mark all bacteria
24. GRAM-POSITIVE BACTERIA stain dark blue or violet. Their cell wall is typically rich with peptidoglycan and
lacks the secondary membrane and lipopolysaccharide layer found in Gram-negative bacteria.
25. GRAM-NEGATIVE BACTERIA do not retain the crystal violet stain because of its physical makeup and will
stain pink
26. The name comes from its discoverer and inventor, HAN CHRISTIAN GRAM (Gram, 1884)
27. NEGATIVE STAINING is a mild technique that may not destroy the microorganisms, and is therefore
unsuitable for studying pathogens.

28-30. DRAW THE STRUCTURE OF EITHER A GRAM POSITIVE OR GRAM NEGATIVE BACTERIA