9 PARA: tiasse7iet PAG. 121 A Fikix Gomer 2,501, Determination of the Peroxide Value (P-V.) (Pifth Edition + Method I1.D.13) 1, SCOPE Thi Standard describes a method for che determinstion of the peroxide value of oils and fats, FIELD OF APPLICATION ‘This Standard is applicable to animal and vegetable oils and fats. DEFINITION The peroxide value is the quantity of those substances in the sample, expressed in terms of milliequivatents of active oxygen per kg, which oxidize potassium iudide under the opecating conditions described. tote, This definition does not agree with the definition given in Method I1.D.13 (Fifth Edition). 4. PRINCIPLE Treatment of the test portion, in solution in acetic acid and chloroform, by a solution of potassium iodide, Titration of the liberated iodine with standardized sodium thivsulphate solution. APPARATUS ALL the equipment used shall be free from reduc ug or oxidizing substances, Vote. Do not grease ground surfaces. 5.1. 3-mi glass scoop. 5.2. Flasks, with ground nacks, ané stoppers, of about 250 ml capacity, dried beforehand and filled with a pure, dry inert gas (nitrogen or, prefecably, carbon dioxide). 5.3. 25 or So-ml burette, graduated in 0.1 ml. REAGENTS 6.1. Chloroform, analytical reagent quality, freed from oxygen by bubbling a current of pute, dry inert gas through it. 6.2, Glacial acetic acid, analytical reagent quality, freed from oxygen by bubbling @ current of pure, dry gas through it. 6.3. Potassium iodide, saturated aqueous solution, recuutly prepared, free from iodine and Hodaten; —_—- 6.4, Sodium thiosulphat ized just before use. 0,01 oF 0.002 N accurately standardized aqueous solution, standerd~ 6.5. Starch solution, 10 g/l aqueous dispersion, recently prepared from natural soluble starch, 7, SAMPLE Toke care that the sample is taken and stored avay from the light, kept cold and contained in cae entate ie #47¢ail lations teeieinene haemertealin aceite aah aseandhladscor cock oxegee™’:8, PROCEDURE ‘The test shall be carried out in diffuse daylight or in artificial light. Weigh in a glass scoop (5.1) vt, Luiling this, in a flask (5.2), to the acacent 0.001 g, a mass of ihe sample in accordance with the following table, according to the expected peroxide value : “Eapected peroxide value aes of coat yortlon (m.e.9-) (s) 0 to 12 5.0 to 2.0 12 te 20 2.0 to 1.2 20 to 30 1.2 to 0.8 30 to 50 0-8 to 0.5 50 £0 90 0.5 co 0.3 Unstopper a flask (5.2) and introduce the glass scoop containing the test portion. Add 10 al ef chloroform (6.1). Dissolve the test portion rapidly by stirring. Add 13 ml of acetic acid (6.2), then 1 al of potassium iodide solution (6.3). Insert che stopper quickly, shake for Viminute, and leave for exactly 3 winutus avay from the ight at a teuperature fron 15 to 25°C. ‘Add sbout 75 al of distilled water, Titrate the Liberated iodine with the sodium thiosulphate solution (6.4) (0.002 N solution for expected values less than 12, and 0.01 N solution for expected values above 12) shaking vigorously, using starch solution (6.5) as indicacor. Carry out two determinations on the same test sample. Garry out similtaneously a blank test. If the result of the blank exceeds “ sodium thiosulphate solution (5.4), replace the iupure cuagents. 205 ml of 0.01 N 9. EXPRESSION OF RESULTS ‘The peroxide value (P.V.), expressed in’milliequivelents of active oxygen per kg, is given by the formula : Pye VEE» 1009 where ¥ is the nunber of ml of the standardized sodium thiogulphate solution (6.4) used for the test, corrected to take into account the blank test. T is the exact normality of the sodium thiosulphate solution (6.4) used. m is the mass, in g, of the test portion. Take as the result the arithsutic mean of the two determinations carried out. FROM: TUPAC Standard Metrode tar +he Anmolvcte uf