ogmere sermeneroo me 1g sintess dele popoiacirids dela pared cesar. Bs sible quel guido ‘FRowra io ce ators decade” promotor se encargu de garantizar a disponbilidad de UDP-galactosa a pesar Prommarsinda con una tocha sagen Ge que lace est unlizand goa como su fuente de ene pincipal, epic gal= gn en prensa 9T= (prelate ono dee HLoperoa RINE ‘ns HLoperén arabinosa presenta otra caracterstica de egulcin poco habitual: la —=_ "operon arabinosa se contola por Esta UnaEpeMAIUEPURHENCWANCOMG ir. GUA REpAEISR METURGONERARABAD. (crys o0 La meena pr un regular dele tanscrnccn Pato onegatho, en uncon des ‘Sth unide on aaa arabinee teina como elemento de canrol postivo y negative es poco comin, per no - rece de precedents, Recuérdese que el teprsor cl de 4 puede atvaroihibic ‘propia sates en faci dels lugares del aperador alos que se una Control mediante RNA antisentido Pra no Tega a la conelsiém de que toda la regulcin de ta transeripidin procarioi simplemente una cuestin de reprsores, operadores yactvadores fe la transeripcion, mencionaremos otros dos mecanismos de regulacion ‘Aunque es ma un rguladorde a tadcion que de la transcrip el RNA “antisentida controle expresiin de varios genes procarioasy eacaiots E RNA sntsentido eel producto de a transctipciin de cadena del DNA com [=x] = [saa T] al SN | U7) BES . =k —— % _@—.. @@ “@ ~@ Lan Goa 3 90,7 ulema laoreet on aa) my neguacon nega eorcenracens de aebnosa bees(2 Pepin psi a concerns oats erat ‘pein re de Ecol) Wn gel Gl opatny Sone omens ‘rues a Race eer gv orp (Sree bace ea Lr e N ga 00 e a ‘Sipe drat pope prt neater coun ici Geounetnbe buwncipe donate canoguesgas ‘Suerte anc esas (comp Asari oun ‘lncr pose deta wascipcn sonar evo ean Sihay Ck ro how Cina comp hat sabre Goring ta varoatn oe oo, cn onto ‘The (AfabinOS@IOpEron: One Regulator, Two Functions Catabolism of the carbohydrate L-arabinose by F, coli involves three enzymes encoded LE LCONUOUNTAANOBNTUNGADAEHES (Fg, 5-9), The product, xylulose- Sephospliate 1y/-p), ca be eitabolized further by entering the pentose phosphate GRIERSNOUINEY in Figure 1-10 (see also Chapter 8). The transcriptional activity Of these genes is coordinately regulated by a fourth Toeus, araC, The araC locus (Fig. 5-10b). Both the araC promoter (Pc) and the araBAD promoter (Pyap) are stimulated by cAMP and CRP. RESUIGOH Sb (270 MiSHUnistal sO Weversaieiiatgiie araC product activates transcription of araP ap in the presence of arabinose (inducing condition) yet represses transcription of both promoters in the absence of arabinose (GEESE! Thus. araC protein can assume two conformations: repressor and activator (Fig. 5-10). The GRIGEPEOUTCHSISS ELCs TOWAEXDIESSIOH ANE presence or absence of arabinose. The arabinose operon was one of the first examples where looped DNA structures were shown to regulate transcription initiationaraC araB araA araD Set 1 t uo ~ Regulatory loci L-Ribulose-5- L-Ribulokinase phosphate- 4-epimerase L-Arabinose isomerase t }. URibulose D-Xylulose L-Arabinose 1 L-Ribulose 5-phosphate S-phosphate cHo Ho CHO HO HCOOH ¢-0 o-0 qo HOCH HOCH HOCH HOCH HOCH HOCH HOCH HCOH r Fi r i CHOH CH.OH CHOPOsH2 = CHeOPO3H2 Fig. 5-9. The arabinose operon.