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You are here: Home / HPLC / Advances in HPLC Stationary Phases: Monolithic HPLC columns
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A monolith can be visualized as a ‘ solid rock’ like structure with no interstitial spaces. These o er TODAY WEEK MONTH ALL
shorter di usion distances and multiple pathways for solute dispersion. The porous rod-like Jetpack plugin with Stats module
structure is highly permeable and provides large surface area for separation in comparison with needs to be enabled.
particle-based stationary phases. Monolithic columns are either silica-based or have organic
polymer backbones.
TOPICS
Historical AAS (53)
Richard Synge in 1952 conceptualized monolith as a continuous block of porous gel. The idea
Analytical Chemistry (3)
picked up in 60s and 70s when polyurethane foams were introduced. It was only in the late 1980s
that hydrophilic gels and silica rods were developed. Calculations (5)
Chemical (2)
Featured (2)
Monoliths with high permeability o er a large number of ow channels and high contact
Free e-courses (2)
area. These features o er several advantages over particulate stationary phases.
Reduction of particle size is accompanied with increase in column backpressure and you are Gas Chromatography (55)
forced to use more expensive UHPLC systems. Backpressure problems are easily overcome
General Topics (140)
when using monolith HPLC columns.
Monoliths are ideally suited for separations of large molecules as such molecules do pose Guest Posts (27)
backpressure complications in particulate columns
HPLC (60)
In– situ polymerized monolith columns avoid column to column variability as well as mobile
ow variations due to wall e ects are minimized. ICP Techniques (13)
Monolith HPLC columns are more rugged and exhibit mechanical robustness in comparison Jobs (1)
to particle packed columns.
Lab Bytes (17)
Particle packed columns need a solvent environment and any exposure to air results in
drying out which can cause irreparable damage. Such problems are not encountered by Lab Management (95)
monolith columns.
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28/04/2019 Advances in HPLC Stationary Phases in Monolithic HPLC columns
Monolith HPLC columns have extended useful life-cycle in comparison with particulate Laboratory Insurance (8)
columns due to increased shock resistance, increased temperature tolerance and ability to
Laboratory Safety (13)
withstand wider pH range media.
Method Validation (1)
News (3)
Due to the multitude of advantages monolith HPLC columns hold promising scope for
Paper Chromatography (10)
applications in biotechnology covering specialized elds such as genomics, proteomics,
metabolomics and pharmacogenomics. Fast response and high resolution of large molecules has pH Measurement (3)
made possible chromatographic real-time monitoring of fermentation processes. Pharma, foods,
Preformulation Studies (3)
forensics and clinical studies are other areas where monolith columns will hold promise in the
coming time. Quality System (9)
and it is possible to use very low ID columns of the order of 20 μm with long column lengths. Stability studies (8)
Training (21)
BIA separations rst introduced polymeric monoliths HPLC Coloumns followed by Merck which
developed and produced silica monoliths with its Chromolith product line.The product line was
subsequently passed on to Phenomenex which also introduced the Onyx line in 2005. Dionex
pioneered polymer monoliths and presently some sources can be accessed through the links
below :
Agilent
Phenomenex
Sigma Aldrich
Thermo Scienti c
Monolith columns holds great promise in the future. In case you have had exposure to such
columns please do share your experience and leave your comments.
Comments
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