NSAIDs. The major adverse event was gastrointestinal symptoms. D.

scandens may be
considered as an alternative for musculoskeletal pain reduction.

1. National drug committee. www.nlem.in.th,The national list of essential drug, A.D. 2015 [in Thai].
The royal gazette (132), 2015 Aug 10.

Tu-Poster Session 2-PO-71:

AMELIORATION EFFECT OF CLINACANTHUS NUTANS ON LPS-INDUCED
NEUROINFLAMMATION IN RATS
Azam Amalina 1, Ismail Intan Safinar 1, Shaikh Mohd Farooq 2, Abas Faridah 1, Shaari Khozirah 1
1
Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang,
Malaysia
2
2Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Subang Jaya,
Malaysia

Neuroinflammation is a pathological hallmark of many neurological disorders associated with

mental health conditions. In the stages of brain immune trauma or infections, an adaptive
response in behavior which resulted from the conserving energy to fight acute inflammation
was observed. We examined the chemical intervention effects on the rat serum metabolites,
and associated with the physiological sickness behavior such as anxiety, exploration and
reduction of locomotor. Metabolic dysregulation in serum of LPS-induced rats was observed at
0 and 14th day after treatment with Clinacanthus nutans (CN) aqueous leaf extracts. CN is a
well-known herbal plant used for its inflammatory remedies. Yet there is no discovery on the
brain inflammation. Hence, 25 rats were undergone unilateral stereotaxic injection of 10µl LPS
(1mg/ml), a toxin potent inducer of inflammation, into the substantia nigra pars compacta.
Another ten rats were induced with phosphate buffer saline (PBS, 10µl) as control. Observation
vice software
(SMART 3.0.1, Panlab Harvard Apparatus) for pre and post- treatment. The impact of
pharmacological interventions of LPS, CN extracts (250, 500 and 1000mg/kg of rats BW) and
control drug (dextromethorphan hydrobromide, 5mg/kg of rats BW) was observed in the 1H
NMR serum metabolites variation by using metabolomic approach. All collected data were
analyzed using Multivariate Data Analysis (SIMCA) from which pattern trend of related group
was documented using orthogonal partial least squares (OPLS). The results revealed that both
behavior and metabolite changes correlated with each other (R2=0.79). A similar ameliorated
striking pattern of correlation between improvement in physiological sickness behavior and
anti-inflammatory biomarker in 1H NMR sera were detected.

Tu-Poster Session 2-PO-72:

Nephroprotective effect of Clinacanthus nutans against cisplatin-induced human
kidney cell (PCS-400-010)
Ismail Intan, Mahmod Ilya Iryani
Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia

Clinacanthus nutans (C. nutans) Lind. locally known as Belalai Gajah or Sabah snake grass is
a medicinal plant from family of Acantaceae. In Southeast Asia, this plant is traditionally used

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