F-ViewII en

Manual
F-View II
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F-View II
The F-View II - camera 4
Acquiring images 6
WHEN CAN THE IMAGE BE TOO DARK? ..............................7

F-View II
CALIBRATING INTELLIGENT EXPOSURE .............................9
EXPOSURE TIME LOCK ........................................................10
Acquiring images with manual control 12
OPTIONS IN THE CAMERA CONTROL ................................15
Online shading correction 17
USING THE SHADING CORRECTION ..................................18

HOW THE SHADING CORRECTION FUNCTIONS ...............18
ACQUIRING REFERENCE IMAGES ......................................18
USE OF THE ONLINE SHADING CORRECTION ..................22
TECHNICAL NOTE .................................................................22
Acquisitions with Real time FFT 23

DFVII0807
NetCam 26
INSTALLING THE WEB SERVER ..........................................27

F-View II
NETCAM IN THE INTRANET ................................................. 27

NETCAM IN THE INTERNET ................................................. 27
USING NETCAM ..................................................................... 28
Setting up the camera 31
LOGICAL INPUT CHANNELS ................................................ 31

THE CONFIGURE INPUT DIALOG BOX ............................... 32
Appendix I: Microscope settings 41
ILLUMINATION ....................................................................... 41
OBJECTIVE, TOTAL MAGNIFICATION AND USEFUL MAGNI-
FICATION ............................................................................... 43
Appendix II: How to install the camera 44
INSTALLATION PROCEDURE - WINDOWS 2000 ................ 45

INSTALLATION PROCEDURE - WINDOWS XP ................... 59
F-View II
In case of problems wih the camera 69
WEEE DECLARATION 70
CE DECLARATION OF CONFORMITY 71
F-View II • The F-View II - camera
F-View II
The F-View II - camera
What is the F-View II is a quick, high resolution CCD color camera used for biomedical
F-View II? and material sciences applications. It sports a special compact design, and
can be connected to all types of light-microscopes via the C-mount. Its high
dynamic scope enables it to acquire images which have very light and very
4
dark areas
Technical data F-View II
Dynamic range 12 bit
Exposure times 100 µs to 160 s
Frame rates 72,3 / s @ Binning 8
39,3 / s @ Binning 4
22,9 / s @ Binning 2
12,4 / s @ full resolution
Resolution options 1376 x 1032,
688 x 516,
344 x 258,
172 x 129
Camera Mount Standard C-Mount
PC-interface Firewire (IEEE 1394)
Overview of the chapters
System Requirements To properly use F-View II, the following system requirements for your
computer and your image analysis system should be taken into consider-
ation:
Hard- / Software Required
CPU P4 with at least 1,8 GHz
Memory 512 MB
Hard Disk Storage 30 GB
Interface Firewire (IEEE 1394)
Operating system Windows 2000 Professional,
Windows XP Pro
Image analysis program analySIS 3.2, Build 625 or higher
analySIS FIVE
cell^A, cell^B, cell^D, cell^F, cell^P
Overview of the chapters

"Acquiring images" on page 6describes the automated acquisition of images
with the help of the Intelligent Exposure function. In the majority of situations
you will be able to use this method to acquire high quality images.
Should you want to acquire fluorescence images, do not attain the required
acquisition quality with Intelligent Exposure, or should you basically prefer
to work with manual controls, the chapter "Acquiring images with manual
control" on page 12 describes how to work with this method.
The F-View II offers an online shading correction feature to counter inhomo-
geneously illuminated images. The chapter "Online shading correction" on
page 17 explains the set up and use of this function.
"NetCam" on page 26 describes the use of the NetCam add-in for making
direct transmissions of images via the Internet or an intranet.
In "Setting up the camera" on page 31 you can learn how to change the
camera's settings, and how you can define different input channels for
different tasks.
"Appendix I: Microscope settings" on page 41 provides you with a short
5
overview of how you select the correct settings for your microscope in order
to achieve the best results.
"Appendix II: How to install the camera" on page 44contains detailed instruc-
tions on how to install the camera under the Windows 2000 and the
Windows XP operating systems.
F-View II Trigger
The use of the F-View II Trigger is the same as with the model without
trigger. The configuration of the trigger functionality is described on page 34.
F-View II • Acquiring images
Acquiring images
Your image analysis program comes with a procedure for automatically
acquiring images. By using Intelligent Exposure you can acquire high quality
images with just a few mouse clicks.
1) Click the Intelligent Exposure button in the Standard button bar.
" Should you use Intelligent Exposure for the first time with this
camera, you will be asked to perform a calibration.siehe Calibrating
Intelligent Exposure auf Seite 9
" The Intelligent Exposure dialog box will be opened.
2) Click the Acquire > button.

" The complete acquired image will be shown in the document
window. Check if the desired image segment is shown in this
setting.
" The exposure times for the live-image and for a snapshot are
displayed in the dialog box's status bar.
3) Find the place on the sample that interests you.
4) Clicking the Focus mode button will enable you to bring the image into
6
focus.
" The central image segment will then be magnified to the point
where one camera pixel is equivalent to one pixel on the image.
This setting enables you to focus optimally.
5) Bring the image into focus.
" To help you do this, you can use the sharpness monitor.
6) Click the Focus mode button to return to the complete image.
7) Then click the Snapshot button to acquire a concluding image with the
settings you have chosen.
" This image will remain available to you even when the Intelligent
Exposure dialog box has been closed, while the live-image will be
deleted.
Related topics
Sharpness Monitor 16
When can the image be too dark?
When can the image be too dark?

Very bright image segments or ones that reflect strongly, lead to the calcu-
lation of a false exposure time.
Intelligent Exposure calculated the exposure time that is necessary by
analyzing the signals. Normally, when this is done, all of the pixels will be
used. Very bright areas in an image, or areas that reflect strongly, cause the
exposure time to be reduced, to also enable these areas to be correctly
displayed. Areas that are not so bright, or don't reflect so strongly, will then
appear to be darker than they are.
This is how you avoid your images being made dark by bright
areas
In this example a graphite sample will be acquired.
1) Start Intelligent Exposure and click the Snapshot button.
" The darker area of the sample will then be displayed very darkly.
The lower part of the image

shows a fibre.
In the default setting,
scratches and inclusions
don't stand out very much
from the background.
This makes an automatic
evaluation difficult.
2) Click the Set ROI for White Balance button.
7
" The mouse pointer appears in the image. On its tip, a red rectangle,
the so-called ROI (Region of Interest) can be seen.
3) Move the mouse to alter the position of the ROI. Change the size of the
ROI by keeping the mouse key depressed and moving the mouse.
4) Position the ROI in an image segment that you can display well.
" While you are moving the ROI, the exposure time will not yet be
changed.
5) Click your right mouse button to fix the size and position of the ROI.
" Intelligent Exposure will then readjust the exposure time to have the
area within the ROI optimally displayed.
F-View II • When can the image be too dark?
When the ROI for the calcu-

lation of the exposure time is
moved to the darker area of
the sample, the sample and
its structures will be brighter,
and more clearly, displayed.
With these settings, an auto-
matic evaluation is made
much easier.
This is how you avoid your images being made dark by strongly
reflecting areas
In this example, a coin with strongly reflecting points will be acquired.
1) Start Intelligent Exposure and click the Snapshot button.
" The sample will be displayed very darkly, since the reflecting points
simulate a bright sample area when the exposure time is calcu-
lated.
With the default setting of

the exposure time correc-
tion, the reflecting areas on
the surface of this copper
coin cause the complete
image to be shown darkly.
The histogram shows that
there are no bright pixels in
the image.
The camera's dynamic
scope will then not be fully
utilized.
8
2) Move the Exposure adjustment slide control to the right.

" The exposure time for snapshots will then be increased. The dark
areas will then become brighter.
Calibrating Intelligent Exposure
Under the same conditions,

the Exposure adjustment
slide control has here been
moved to the right. This has
lead to a noticeable
improvement in the contrast.
Intelligent Exposure calcu-
lated an exposure time that
was twice as long.
The histogram shows that
the camera's dynamic
scope has been utilized
much better.
Calibrating Intelligent Exposure

Before you use it for the first time, you have to calibrate Intelligent Exposure.
The calibration will be calibration automatically set off when you start Intelli-
gent Exposure for the first time.
Intelligent Exposure arranges linear correlation between the exposure time
and the mean intensity for calculating the optimal exposure time. In order to
take advantage of this correlation, the camera's dark current must be known
as an offset of the linear function. Thus, the first step to working with Intelli-
gent Exposure is measuring this dark current via the acquisition of an image
using a darkened camera. When first starting Intelligent Exposure, you will
be automatically asked to carry out this calibration.
9
This calibration step measures the camera's dark current, in order to make
an offset correction possible. After successful measurement of the dark
current, Intelligent Exposure asks you to take the next step.
F-View II • Exposure Time Lock
The second calibration step consists of measuring the signals at various

camera resolutions. In this way the camera's sensitivity will be determined.
The live image shown changes considerably during this step.
Calibration problems The camera can be overexposed during calibration due to bright illumina-
tion. In this case you will be prompted to decrease the illumination. If the
overexposure warning remains active in spite of decreased illumination, you
can deactivate the camera's binning modes. Use the command Image > Set
Input > Configure Device to achieve this.
Repeating the The calibration is specific to each camera.
calibration Should you change the camera, you will have to perform the calibration once
more. You have access to this function by using the Calibrate... button
located in the Intelligent Exposure > Options dialog box.
Exposure Time Lock

Exposure Time Click the Exposure Lock button in the Intelligent Exposure dialog box to lock
Lock the current exposure time. From this moment on, no more changes will be
made to the exposure time, even if you shift the Exposure time slide control.
Click the button anew to return to the dynamic adjustment of the exposure
time.
Use the Exposure Lock option when you want to compare the intensity of
different images or when you want to combine them. You will want to
combine separate images, for example, when you use the MIA add-in, with
which the images are combined into one composite image.
Modify contrast
Use the buttons Reduce contrast and Increase contrast to modify the
contrast during live acquisition. When you modify the contrast, the online
histogram will be shown and the limits of the fixed scaling will be marked.
Modification of the contrast has an influence on the limits for fixed scaling.
Online histogram without
contrast modification.
The shaded area on the left
10
side of the histogram will be

shown black, the shaded
area on the right side will be
shown white.
Reduce Click on the Reduce contrast button to reduce the contrast during live acqui-
contrast sition. Reducing the contrast means shifting the upper limit of the histogram
to the right, and the lower limit to the left. The intensity regime shown will be
increased, thus reducing the contrast. Very light and very dark regions,
formerly shown uniform black and white, respectively, will now be displayed
differentiated.
Related topics
Device Properties 32
Modify contrast
Online histogram with

reduced contrast.
shown white.
Increase Click on the Increase contrast button to increase the contrast during live
contrast acquisition. When increasing the contrast, the upper limit in the histogram is
moved to the left and the lower limit is moved to the right. The intensity
regime shown will be increased, thus reducing the contrast. The image
generally appears lighter. All of the displayed image sections appear richer
in contrast. Very light sections of the image appear uniformly white and very
dark areas appear black.
Online histogram with
increased contrast.
shown white.
11
F-View II • Acquiring images with manual control
Acquiring images with manual control

The methods that have been described for making acquisitions using Intel-
ligent Exposure already cover a wide range of possible applications in
brightfield microscopy. For high quality fluorescence acquisitions you should
use manual controls, however. These allow you to work fast and avoid
stress to the specimen.
The following steps describe a typical workflow for fluorescence acquisi-
tions:
Initial considerations
The F-View II acquires images with a dynamic range of 12 bit. The monitor
can only display a dynamic range of 8 bit. This means that the image
contains more information than can be displayed.
Preparing the input channel

1) Use the Image > Configure Input... command.
" The Configure Input dialog box will be opened.
2) Select the display tab.
3) In the Image scaling picklist, select the way you want the camera image
to be displayed on your monitor.
" For examining the sample, select the Adjust to viewport option.
With this setting you will see the camera's complete image on your
monitor while you are making the acquisition. It is suitable for
moving to the area of the sample that interests you.
" To focus, select the Full size (100%) option. With this option each
monitor pixel is equivalent to one camera pixel.
4) Select the Online histogram check box.
5) Select the Automatic gain display check box and enter a value of 0.01%
in the Right overflow field.
" With a higher value for the right overflow, bright points of the fluo-
rescence acquisition might not be correctly differentiated.
6) Click the OK button to close the Configure Input dialog box.
12
Acquiring images with manual control
Acquiring a live-image
7) Click the Camera Control button in the Standard button bar.
" The Camera Control dialog box offers you all of the possibilities for
making settings for the camera.
8) Click the High Sensitivity Mode on/off button in order to activate the
signal amplification of the camera.
" In High Sensitivity mode, an amplification of the analog signals of
the camera takes place. The High Sensitivity mode allows you to
work with shorter exposure times, which is helpful while searching
a position on the specimen and focussing. The Sensitivity Mode
results in increased noise, however.
9) Click the Automatic gain display and the Histogram calculation on
whole image buttons.
" This setting ensures that the following steps (searching the desired
position on the specimen, focussing) can be performed with a very
short exposure time.
10) Click the Acquire button and open the shutter on the microscope.
" The live-image is displayed on the monitor.
" The online histogram will be displayed.
11) Click the Auto button, located in the Camera Control dialog box, to have
the exposure time automatically set correctly.
" Alternatively, you can change the exposure time by entering the
13
values you want, or by clicking the [+] and [-] keys.
12) Search for the desired position on the specimen and focus the image.
F-View II • Acquiring images with manual control
Image acquired with active Automatic gain display and High Sensitivity Mod. The histogram shows that only half of the
camera's dynamic range has been utilized.
13) Click the Use fixed scaling button to deactivate the automatic gain
display and use the method fixed scaling instead.
" This makes it possible to compare the intensity of images that have
been acquired with the Fixed scaling option.
14) Click the Automatically adjust fixed scaling button to have the specified
area adapted to the current conditions.
" With this setting you can compare the intensities of different images
to each other.
15) Click the Snapshot button to acquire a snapshot with the current
settings.
14
Related topics
Automatic Gain Display and fixed scaling 15
Options in the Camera Control
Options in the Camera Control

Automatic Gain Display and fixed scaling
Automatic gain display and fixed scaling are both methods of transforming
the crude camera data into an image that can be displayed. While the
camera works with a resolution of 12 bit, for the display, storage and further
processing, only 8 bit can be used.
Automatic Gain The Automatic Gain Display carries out the adaptation dynamically
Display according to an analysis of the histogram. In this way the image quality will
be automatically adapted when changes are made in the illumination or the
sample. It is, however, not possible to compare separate images that have
been acquired with this setting, where their intensity is concerned.
What counts for the Automatic Gain Display, is the area on which the histo-
gram has been calculated.
Button Area Effect

Full image The histogram is calculated on the complete image.
This option is recommended if the structures to be observed
are equally dispersed throughout the entire image.
Crosshairs The histogram is calculated according to a strip that runs in
vertical and horizontal directions in the form of a cross, and is
only a few pixels wide.
ROI The histogram is calculated on a rectangular area that is de-
fined by clicking on the Set ROI for Histogram button.
This option is suitable when the reproduction of a part of the
image is to be optimized.
Fixed scaling With the fixed scaling option the setting that has been made will not be
altered. This makes it possible to compare the intensity of images that have
been acquired with the Fixed scaling option.
Gamma Setting
15
Click the Gamma Setting button to have a nonlinear contrast enhancement
be performed.
What is gamma? The numerical value for gamma effects how the illumination intensity of the
pixels of the camera CCD chip is converted into the gray-value or the corre-
sponding pixel in the image buffer. If gamma equals 1, the conversion from
illuminance into gray-value is linear. If gamma does not equal 1, this results
in a nonlinear contrast enhancement. If gamma is smaller than 1, the
brighter gray values will be spread more significantly than the darker ones.
The result will be enhanced contrast in the lighter areas of the image. The
image as a whole will become darker. If gamma is greater than 1, the darker
gray values will be spread more significantly than the brighter ones. The
result will be enhanced contrast in the dark image areas. The image as a
whole will become lighter.
F-View II • Options in the Camera Control
Gamma Use the Gamma slide control to obtain a nonlinear contrast enhancement.
Please note that this gamma filter does not only affect monitor display but
also alters actual image information. If you are planning on quantitatively
evaluating your images at a later time, use the value 1.0 for the gamma filter.
The Oper menu provides you with commands for adjusting image contrast
at any later time.
Default Click the Standard button to reset gamma to 1.
Sharpness Monitor
The Sharpness Monitor allows you to control the focus settings during a live
acquisition. For the sharpness monitor you can define a rectangular area to
be analyzed. The maximum contrast serves as a monitor for the sharpness
in this area. Therefore this area should be limited to an image segment in
which the contrast is a good monitor for the sharpness.
The Sharpness Monitor consists of a dialog box in which a relative measure-
ment of the sharpness is displayed by a changing bar which can be varied
between Blurred and Focused.
If you start a live acquisition with an activated Sharpness Monitor or activate

it during a live acquisition, it will show middle sharpness (50%) in each case.
16
This is because there is no absolute measurement for the sharpness in an

image and because the sharpness monitor only gives relative values which
are related to the initial situation which was present when starting the live
acquisition.
Focussing and Sharp- The length of the bar changes if you alter the focus settings during acquisi-
ness Monitor tion with an active sharpness monitor. In doing so, the blue marking bar
shows the maximum sharpness reached since the live acquisition was
started. The bar shifts accordingly to the right should the sharpness increase
due to your changes. If the focus settings are already optimal when starting
acquisition, the bar can only shift in direction Blurred. The smallest sharp-
ness value will be indicated by a green marking bar.
Reset to 90% Click the Reset to 90%button to set the sharpness monitor's relative value
to 90%. Utilize this function if you start working with a very blurry image and
if the sharpness monitor's bar reaches the limit to the right while focusing.
By resetting to 90%, the display gets more room for additional focussing.
The Reset to 90% function is also available if the sharpness drops very dras-
tically due to changes made to the microscope settings (i.e. changing objec-
tives) during an acquisition in progress.
Online shading correction
Using the sharpness monitor

1) Set all other parameters (microscope settings, exposure time, white
balance) optimally. The application of the sharpness monitor should be
the last step before image acquisition.
2) Acquire a snapshot.
3) Use the Camera Control command.
4) Click the Set ROI for sharpness monitor button to interactively define a
rectangular area of the image for the sharpness monitor.
" To do this, select an area of the specimen for which a good sharp-
ness setting is especially important.
5) Begin a live acquisition.
" The Set ROI for sharpness monitor button becomes inactive.
6) Click the Sharpness Monitor On/Off button to open the Sharpness
Monitor dialog box.
" The sharpness monitor begins with a value of 50%.
7) Vary the focus settings and observe the sharpness monitor while doing
so.
" The sharpness monitor bar shows the current sharpness, the blue
markation shows the highest achieved sharpness.
" Mind the exposure time when setting the focus. The sharpness
monitor alters itself with a certain amount of delay when using
larger exposure times (> 100ms). Vary the focus settings in small
intervals and wait each time until the sharpness monitor has been
adapted as well.
8) Find the focus settings by which the sharpness monitor is at its
maximum and use these settings for the acquisition.
Online shading correction

Every optical system with a camera and microscope creates, even when the
devices have been set up very carefully, an effect called shading.
The left illustration shows an
17
image where the online
shading correction has not
been activated. The image
intensity decreases towards
the edges.
Shading Correction For a shading correction, this effect will first be determined to enable correc-
tions to be made to the acquired images. Using the online shading correc-
tion of F-View II, the shading correction already takes place in the live-
image.
F-View II • Using the Shading Correction
Using the Shading Correction

When your microscope had been precisely set up, you will hardly be able to
perceive any shading where snapshots are concerned. The use of a shading
correction may, in this case, be advisable, but is not essential. When,
however, several snapshots are combined, as for example, when you use
the MIA add-in, then even mild shading will immediately become visible.
With fluorescence acquisitions, the shading correction generally brings no
advantages.
How the Shading Correction functions

The correction of the image takes place pixel by pixel with the help of two
different reference image series: one made up of dark images for each of
the camera's resolutions, the other of correction images. The online shading
correction will not be ready for use until you have acquired these reference
images.
Dark Images A dark image is an acquisition in which no light falls on the camera. Noise or
defective pixels may here produce signals which can be corrected by the
dark image. Each of the camera's resolutions requires its own dark image.
Correction Images The illumination of the entire optical system without the object (with refer-
ence samples in reflected light mode) is shown. In addition to the camera
characteristics, the microscope's optical characteristics, especially the
objective being used, contribute to the correction image. Accordingly, a
correction image must be acquired for each camera resolution and objective
combination.
Acquisition Methods As well as the already mentioned influences that camera and objective
exert, the acquisition process you use also plays a vital role. The online
shading correction will have been aligned with transmitted light, reflected
light, DIC and Phase Contrast microscopy. When acquiring the correction
images, you will be asked which acquisition processes have been set.
Should you want to employ more than one process, you will have to repeat
the acquisition of the correction image for each process used. If, while you
are using the program, you change the acquisition process, you will also
18
have to change the Online Shading-Correction's setting.

Note When using a magnification changer, correction images must be acquired
for each possible total magnification.
Acquiring Reference Images

Note Before you acquire reference images and activate the shading correction,
make sure that your microscope is correctly adjusted and that the objective
is clean.
Please be very careful when acquiring reference images for the online
shading correction, since false correction acquisitions can lead to artifacts in
the images. The actual acquisition process is controlled by a wizard. This
wizard tells you which steps to take.
Prerequisites for a refer- A reference acquisition always relates to one particular magnification, for
ence acquisition this reason, before the reference image is acquired, an XY-calibration of the
objective to be used will have to be performed. The calibration that you can
Related topics
Illumination method 33
Acquiring Reference Images
perform when your image analysis program is installed, fulfills this require-
ment.
As part of the preparations for making the acquisition, the wizard asks you
if the correction acquisition is to be made only for the current magnification
or for all calibrated magnifications.
Transmitted Light 1) When using a tungsten-halogen lamp, set the same lamp voltage as
Mode when using normal operation.
" The lamp voltage should not be less than 5V. Use ND-Filters to
reduce possible brightness.
Note ND-Filters belong to the basic components of the microscope. They will be
brought into the light path to reduce brightness without altering the color
temperature. Should your microscope not have an ND-filter or not provide
for the installation of one, you will instead have to reduce the lamp voltage.
2) Set the illumination method to brightfield. This also holds true if you
want to use special methods at a later time.
3) Bring the objective with the smallest magnification, by which the
condenser front lens still has to be used, into the light path and set the
Köhler illumination.
4) Set the camera's exposure time to a value of > 50ms by lowest resolu-
tion.
" The value in the online histogram should be around 2,000.
The online histogram shows
the correct setting for the
acquisition of the gain
image.
5) Bring the object into the light path and bring it into focus.
6) Defocus by increasing the space between the objective and the object
19
until the object is recognizable as a whole but the details of the struc-
ture are no longer visible.
7) Move to a position on the slide where neither the sample is visible, nor
are there any scratches or contamination.
" Should such a position not be able to be found, remove the micro-
scope slide from the microscope and acquire the reference images
without the object.
8) Begin the acquisition of the reference images with the Image > Acquire
Reference Images... command.
" The Online Shading-correction dialog box will open.
9) Follow the wizard's instructions to acquire the reference images.
Reflected Light Mode 1) When using a tungsten-halogen lamp, set the same lamp voltage as
when using normal operation.
" The lamp voltage should not be less than 5V. Use ND-Filters to
reduce possible brightness.
Related topics
Köhler Illumination 42
F-View II • Acquiring Reference Images
Note ND-Filters belong to the basic components of the microscope. They will be
brought into the light path to reduce brightness without altering the color
temperature. Should your microscope not have an ND-filter, please contact
your supplier.
2) Set the illumination method to brightfield. This also holds true if you
additionally want to use special methods at a later time.
3) Bring the objective with the smallest magnification, by which the
condenser front lens still has to be used, into the light path and set the
Köhler illumination.
4) Set the camera's exposure time to a value of > 50ms by lowest resolu-
tion.
" Your online histogram's gray value should be around 2,000.
The online histogram shows
the correct setting for the
acquisition of the gain
image.
5) Bring the reference sample into the light path and set the Köhler illumi-
nation.
" The reference sample must be color neutral and have a flat surface
devoid of defects or optical structures visible through the micro-
scope.
Note Should you not have access to a reference sample, you can also use a piece
of opaque glass. In this case, take special care that you move it out of focus.
6) Increase the distance between the objective and the reference sample
until the surface structures of the reference sample are no longer
visible.
Warning Please note that the focus depth is high when you use objectives with lower
20
magnification. In this case, you have to defocus more than with objectives
with a magnification of 10x or higher.
When using reference samples with transparent surfaces, it is important to
be sure that no plane within the reference sample is in focus.
7) Begin the acquisition of the reference images with the Image > Acquire
Reference Images... command.
" The Online Shading-correction dialog box will open.
8) Follow the wizard's instructions to acquire the reference images.
9) Carry out a new white balance when you first use the online shading
correction.
Enabling Shading Correction

When you have finished acquiring the reference acquisitions, you will be
asked if you want to activate the online shading correction. Independent of
the decision you make right then, you can activate or deactivate the online
Related topics
Köhler Illumination 42
Repeat or Update the Reference Acquisitions
shading correction on the Image > Configure Input > Input tab at any time.
The settings of the online shading correction remain even after the program
has been restarted.
Input channel and The shading correction's reference images are valid for one particular
shading correction camera. They will also be used for other input channels that have been set
up for this camera. The fact, whether the online shading correction has been
activated or not, is, on the other hand, a property of the input channel. Where
newly set up input channels are concerned, the shading correction is not
activated by default. In the case of a duplicated input channel, the shading
control's setting will be adopted from the input channel that has been dupli-
cated.
Repeat or Update the Reference Acquisitions

The online shading correction functions optimally as long as the illumination
settings are not changed.
Sample acquisitions Sample acquisitions enable you to check the quality of the online shading
correction. To do this, acquire an image of a sample with a background that
is as even as possible. Then measure the acquired sample's intensity distri-
bution with the commands in the Measure >Intensity Profile submenu. When
the shading correction has been correctly set, the intensity profile will,
except for the sample's structures, be even.
Example of an intensity
profile without shading
correction (or with a poorly
performed one), and with
shading correction.
The intensity profile shows a
clear decline along the X-
axis.
21
Alterations to the illumi- If you switch to another illumination method which is supported by the online
nation method shading correction, you may have to make new correction acquisitions.
Should the required acquisitions already exist, you can set the appropriate
illumination method with the Image > Configure Input > Input command. To
do this, select the appropriate entry from the Illumination method list.
Related topics
New Channel 31
F-View II • Use of the Online Shading Correction
Adding/changing objec- If you add new objectives or change already installed objectives, you must
tives acquire correction images for the new objective.
Altering the lamp voltage Small alterations made to the lamp voltage do not influence the online
shading correction. Larger alterations cant be compensated by the online
shading correction. Therefore, use the same lamp voltage you utilize in your
normal routine use, when you acquire the correction images. Should you
need to reduce the brightness, use ND filters. Otherwise you will have to
acquire new correction images for the shading correction.
Use of the Online Shading Correction

Please note the following instructions for the use of the online shading
correction in order to be able to make optimal acquisitions.
Before acquisition Make sure that the optical system is clean. Check the lamp voltage and
check if the Köhler illumination has been set. Furthermore, there should not
have been any changes made on the microscope's light path since the last
reference images were acquired.
Setting magnification The correct magnification must be set for the use of the online shading
correction.
Automatic microscope It is not necessary to set the magnification when using an automatic micro-
scope which is remote controlled by the application.
Technical Note
Organizing the reference Dark images and correction images are saved to the hard disk. In order to
images be able to work with a correct online shading correction when having
changed the camera, the image analysis program creates an individual
directory for saving the reference images. This directory is in the image anal-
ysis program's working directory. Its name begins with DGSISFW and
continues to contain information about the serial number of the camera
being used. This directory should not be moved or renamed.
The reference images file names are also coded in such a way that the
online shading is able to automatically access it. Should one or more of the
reference images have been deleted, the application immediately tells you
22
that a missing image is required and automatically requires you to reacquire

the missing reference image.
File Format The reference images are saved as normal TIF images.
Requirements The filter in front of the camera's CCD chip and all of the microscope's
optical parts must be clean. Otherwise, dust particles or fingerprints would
appear in the correction images. The shading correction can, of course,
correct such contamination, as long as they remain unchanged. Should a
contaminant be moved or removed, the shading correction would give you
a ghostly image of the former contamination in each new image. Check the
filter before acquiring the reference images and clean it with a LensPen, if
necessary.
Intelligent Exposure and Online Shading Correction

The Intelligent Exposure acquisition method works problem-free together
with the online shading correction. In some cases, however, the shading
correction can result in over exposure. You will then receive an error
message when executing a white balance.
Acquisitions with Real time FFT
Adapting Intelligent In this case, click the Options...button located in the Intelligent Exposure
Exposure dialog box. Reduce the Dynamic limit in %value in the Intelligent Exposure
Options dialog box to approximately 70.

Enabling Real time FFT You have to mark the Image > Configure Input > Display > Live Overlay and
Image > Configure Input > Display > Real time FFT check boxes to utilize
real-time FFT during live acquisitions.
Real-time Fast Fourier Transformation (RTFFT) calculates the acquired
image's Fourier image in real time (the absolute value of the Fourier trans-
forms). The Fourier image is continually updated during a live acquisition. It
can serve as a quantitative display of the image quality. For example, if you
blur the microscope image, the high frequencies disappear in the Fourier
image (i.e. the structures which are located farther away from the center).
The Fourier Image of a
Catalase 2-D Crystal.
The points represent peri-
odic structures in the micro-
scope image. The closer
such a point is to the center,
the smaller the spatial
frequency of the corre-
sponding periodic structure,
i.e. the larger the periodic
distances in the microscope
image. (Fringe effects cause
the cross to appear.)
To achieve optimum on-screen display of the fourier image, use the RTFFT
Control dialog box with the tabs ROI, Display, and Tools.
23
ROI
Use the ROI tab to select the area, on which the RTFFT is to be carried out.
The RTFFT is calculated on a square segment of the image. Two different
sizes are available for the calculation. You can also position the square that
is to be analyzed anywhere in the image.
F-View II • Acquisitions with Real time FFT
Note In case the sizes offered you are still too small, you can activate binning.
This increases the size of the ROI compared to that of the image segment.
256x256, 512x512 Select the option 256x256 or 512x512 to make the ROI the respective size.
Show ROI Select the Show ROI check box to display the position of the ROI in the live-
image. The ROI is displayed as a red frame.
Position Click on the Position button to move the ROI in the live-image. The RTFFT
Control dialog box will be hidden and the pointer appears in the live-image.
The red frame shows the position of the ROI for the RTFFT. Move the
mouse to alter the position of the marked area within the image document.
Click the right mouse button to fix the position of the ROI.
Display
Use the Display tab to modify the monitor display of the RTFFT.
Contrast The Contrast slide control enables you to alter the contrast of the display in
the RTFFT Display window. Move the controller to the right to increase the
contrast and to the left to decrease it. Should you have selected a Display
palette the colors will change in the RTFFT Display window. You define the
palette in the input located on the Display tab.
Brightness The Brightness slide control enables you to alter the brightness of the Real
time FFT display in the RTFFT Display window. Move the controller to the
right to increase brightness. Move the controller to the left to decrease
24
brightness. Should you have selected a Display palette the colors will
change in the RTFFT Display window.
Reduced FFT Select the reduced FFT check box to further reduce the image section on
which the FFT will be calculated. At the same time the display in the RTFFT
Display will be enlarged.
Related topics
Display palette 38
Tools
The Tools tab offers RTFFT-related tools.

90° Rotation Select the 90° Rotation to alternate the FFT display between the usual orien-
tation and one rotated through 90°.
Overlay Circle Select the Overlay Circle check box in order to show a marking circle in the
RTFFT Display window. The circle will be shown in the center of the window.
With its help you can position the camera or sample.
Circle Size Move the Circle Size slide control to the right to increase the diameter of the
overlay circle. Move the Circle Size slide control to the left to decrease the
diameter of the overlay circle. The circle size is represented in pixels in the
RTFFT Display window.
Grab FFT Click the Grab FFT button to save the content of the RTFFT display window
as a single image. The image will be written into the next image buffer. This
image's name is that of the live-image, supplemented by 'FFT(8bit)' and a
consecutive number.
When you acquire a snapshot as a conclusion of the live acquisition, such an
FFT image will also be created and saved.
25
F-View II • NetCam
NetCam
You can export the F-View II live-image to other computers via an intranet
or the Internet, by using the NetCam add-in.
This enables you to make the images immediately available to your
colleagues during an acquisition. This makes it possible for you to work
interactively over large distances by using simultaneous communication,
e.g., telephone. The images will be compressed in the process to speed up
the rate of transmission.
Transferred images The live-image will be directly transferred. Provided your network connec-
tion achieves an adequate speed and the frame rate attained during acqui-
sition, is sufficient, you will get the impression of a live-image. In an internal
company network, an immediate feedback without any time delay will be
possible between observer and user.
NetCam can also transfer a fixed image, whereby in this case the image's
overlay will also be transferred.
What is a Server? A Server is the computer on which both the image-analysis program and the
NetCam are installed. The image acquisition is controlled from the server.
What is a Client? A Client is any computer that is able to access the server's images via the
Internet. The client cant control image acquisition.
A web server must be installed on your computer to make images available
via the Internet. An appropriate program will be installed during the setup, if
you have selected the NetCam add-in. This step will be skipped should a
web server already exist on your computer.
Warning When you use a computer connected to a network it is possible that the
security settings will not allow its use as a web server. In this case, contact
your system administrator.
In your image analysis program's Module\NetCam subdirectory you will find
the file "NetCam_Info.pdf". This file contains technical explanations about
NetCam which you can give to your system administrator.
26
Installing the web server
Installing the web server

The web server you require for using NetCam will be automatically installed during the setup.
The installation of the web server is started directly as part of the setup. After
you have agreed to the license terms, you can make further settings in a
dialog box.
You can simply adopt the default settings.

Complete, Custom We recommend the complete installation of the web server.
NetCam in the intranet
27
The transfer of images within the company network or intranet generally
does not require any further actions.
Enabling access During installation, the setup will create a link with the name NetCam.html in
the \Module\NetCam subdirectory below your image-analysis program's
working directory. Send this link via E-mail to colleagues you would like to
give access to your images. The easiest way to do this is with the following
command: Send to > E-mail receiver in the context menu of the link in
Windows Explorer.
Prerequisites for clients Java Runtime Environment, Version 1.4.1_03 or higher is required to show
the images on the clients. When this requirement has not been fulfilled by
the client, he will not be able to receive the images.
NetCam in the internet

When using NetCam, a direct communication takes place between the
server and the client. If the communication goes beyond the company
network, you must, if need be, make changes to the security settings.
F-View II • Using NetCam
Adapting the security The adaptation of a network's security settings takes place via the respec-
settings tive system administrator. The necessary technical and security require-
ments for the use of NetCam are outlined in a separate file entitled
"NetCam_Info.pdf" which will have been saved during setup to your image
analysis program's \Module\NetCam subdirectory. Please give this file to
your system administrator so that he can make the appropriate adaptations.
IP Address The server's internet address must be entered on the client's page. This
address is available in the well-known alphanumerical form ("MyCom-
puter.OurCompany.de") and in the numerical form ("62.180.61.137"). The
numerical form of the address is more reliable, especially if your system
administrator has to alter the network settings.
Determining the IP You can find out your computer's IP address from your system administrator
address or determine it yourself.
Determining the IP address

1) Select the operating system command Start > (All) Programs > Acces-
sories > Command Prompt.
" The Command Prompt console box will be opened.
2) Enter the ipconfig command via your keyboard and use the enter key.
" Data about your computer's network connection are shown in the
console box.
The command will display a
computer's IP address
28
3) Note the entry in the IP address row.

4) Supply the users who are to have access to your server as NetCam
clients with this IP address.
Prerequisites for clients Java Runtime Environment, Version 1.4.1_03 or higher is required to show
the images on the clients. When this requirement has not been fulfilled by
the client, he will not be able to receive the images.
Using NetCam
The use of your image-analysis software remains the same when using
NetCam. You can use all of the functions as always. To transfer the images
to other users and to be able to control the transfer, use the buttons located
in the NetCam button bar.
Using NetCam
NetCam on/off Click the NetCam On/Off button to start or end the transfer of images with
NetCam.
The transfer of live-images begins as soon as NetCam is activated. The
transfer continues until you deactivate NetCam or close your image-analysis
software.
NetCam Click the NetCam Settings button to set image options and to administer the
Settings clients which are to have access to your images. The NetCam Settings
dialog box is opened.
Connections
Use the Connections tab to administer the clients which are to access your
images.
Connected Clients In the Connected Clients list you will see all of the clients which are currently
connected with your computer. You can mark one of these clients to discon-
nect the connection to it or to refuse a connection in the future.
Disconnect Click the Disconnect button to disconnect the connection to the marked
client. The client can once again make a connection.
>> Click the >> button to include the marked client in the Banned Clients lists.
29
The corresponding client is automatically disconnected.
Banned Clients In the Banned Clients list you will see all of the clients who you have
forbidden access to your image-analysis software. From these computers it
is impossible to load and view the images made available by NetCam.
Add new... Click the Add new... button to add computers or entire groups of computers
to the Banned Clients list. The Enter IP(s) or a domain dialog box is opened.
F-View II • Using NetCam
Domain name Enter the alphanumerical name of a domain in the Domain name field if you
want to block the access of all computers belonging to a certain domain. For
example, enter the domain name foobar.com to block the transfer of the
images to all of the computers within the foobar.com domain.
Procedure NetCam sends a query to the nameserver to get the numerical IP addresses
belonging to the specified domain. Should the query lead to an error, you will
receive a message. The specified domain will, in this case, not be placed on
the Banned Clients list.
IP Address(es) You can provide a numerical IP address or an entire address range in the IP
address(s) group to exclude the computers in question.
From, to Enter the limits of the address range to be excluded in the from and to fields.
Image options
In the Image Options tab you can set the quality of the transferred images
and thus influence the transfer rate.
Image quality Use the Image Quality slide control to control the compression of the
images. Move the slide control to the right to maintain a high image quality.
The data amount of the transferred images increases, whereby the transfer
30
rate decreases. Move the slide control to the left to increase the compres-
sion. The image quality decreases, whereby the transfer rate increases.
Image quality and data A single image of F-View II can be of a size up to 2.8 Mbyte. With an image
transfer rate of this size, the transfer to a client can take a long time, even if you're using
a very quick network connection; the effect of a live-image being transferred
will be lost. For this reason, images are compressed for transfer. The algo-
rithm is equivalent to that used with JPEG images. Thus, high compression
rates are achieved, whereby, however, image quality decreases.
The amount of compression achieved is based on the image type. Images
with large, uniformly colored areas can be compressed more than detailed
images without these characteristics.
Setting up the camera
Setting up the camera

Logical input channels
For every camera that is to be used with the image analysis program, one
or more logical input channels will be set up. Logical input channels contain,
for example, the information about how large an image format is, and what
is to happen with the image signal before it reaches the image buffer. Each
channel must be calibrated separately. A channel for acquisitions with the
F-View II is typically called "F-View II FW ...".
Status after installation During the installation of the software, one input channel will automatically
be set up, which you can use to acquire images immediately.
Precalibration During the installation you will be offered the opportunity of performing an
XY-calibration by entering the objectives and the TV adapter to be used.
Thanks to this automatic calibration you can then acquire calibrated images
without any further preparations.
Configuration via tabs Input channels are configured via the inputting of suitable values in various
tabs. The 3 view tabs relevant for F/View II will be dealt with in-depth.
Setting up additional You can set up and use more than 100 additional input channels. This can
input channels be useful when you want to employ different settings for a variety of uses. If
you have to set up a new input channel, you can create a copy of an already
existing input channel and adapt it, or create an entirely new input channel.
Set Input Use the Image > Set Input... command to select a new input channel or to
create a new one. You can also depress the [F6] key. The Set Input dialog
box will be opened.
The Set Input dialog box
with the already defined
input channel.
31
New Channel Click the New Channel button in the Set Input dialog box to create an entirely
new input channel. The Select device dialog box will be opened. All
connected image creating devices are listed in the Available devices list.
F-View II • The Configure Input dialog box
Select Device dialog box

with a connected F-View II
camera.
Mark your camera in the Available devices and click the OK button, in order
to define a new input channel.
Creating additional input Should you want to create additional input channels, for example to have
channels different configurations immediately available for repeating tasks, use an
already existing input channel.
Duplicate Click the Duplicate Channel button to make a copy of the active input
Channels channel. The new input channel gets the same name as the channel from
which it was copied and receives a sequential number. The newly created
channel is automatically enabled.
Configure Click the Configure Input button to adapt the settings for the new channel.
Input The Configure Input dialog box will be opened. You can also open this dialog
box by using the Image > Configure Input command or by double clicking
the camera name located in the status bar.
Device Proper- Click the Device Properties button to disable 8x- and perhaps 4x-binning as
ties well. The acquisition process Intelligent Exposure utilizes all binning modes
of the camera. Due to a high illumination intensity the calibration of Intelli-
gent Exposure may fail, due to the camera being overexposed.In this case,
you can disable 8x-binning at first and, if necessary, 4x-binning as well.
32
The Configure Input dialog box

In the Configure Input dialog box you can make various setting for the input
channels. These settings are saved for each input channel separately.
Related topics
Calibrating Intelligent Exposure 9
Input tab
Input tab
Exposure Enter the number of microseconds the camera's CCD chip is to be exposed
in the Exposure field. You can also use the arrow key (to the right of the field)
to select this value. The chapter entitled "Camera Control" explains how you
can change the value in this field with the help of the Image > Camera
Control... command interactively while viewing a live-image. The smallest
possible exposure time is 100 µs and the largest is 160 s.
Shading Correction You can set the camera function with the same name in the Online Shading-
correction group.
Dark image / Correc- Select the dark image check box to use the background correction. Select
tion image the Correction image check box to use the correction images for the shading
33
correction. For complete shading correction, both check boxes have to be
selected.
Illumination method Select the procedure you use in the Illumination method list.
Mirror The Mirror group enables you to determine if the camera's image is to be
mirrored directly into the image analysis program during transfer. Both
mirror functions can be combined with each other.
Horizontal Select the Horizontal check box to mirror the image horizontally during
acquisition.
Vertical Select the Vertical check box to mirror the image vertically during acquisi-
tion.
High Sensitivity Mode Select the High Sensitivity Mode check box to activate the signal amplifica-
tion. You can activate this mode via the High Sensitivity Mode on/off in the
Camera Control dialog box as well.
Related topics
Dark Images 18
Correction Images 18
Acquiring a live-image 13
Trigger Use the Trigger group to set up the trigger functionality of the camera. The
Trigger group is only available with the F-View II Trigger model of the cam-
era. The basic F-View II model doesn't offer this functionality.
Trigger mode Select an operation mode Trigger mode list.
Trigger in Select the mode Trigger in to control the camera using an external trigger.
As soon as the F-View II Trigger receives a valid trigger signal, a single im-
age will be acquired. You can use, e.g., a short circuit switch to acquire an
image via remote control in this mode.
Trigger out Select the Trigger out mode to utilize the camera as trigger for other external
devices. As soon as you make an acquisition, a trigger signal will be issued.
In this mode you can, e.g., synchronize the illumination control with the im-
age acquisition.
Trigger polarity Select the flank to be used for triggering from the Trigger polarity list. You
can select either a positive (rising) or negative (falling) flank.
Info Click the Info... button to have a variety of device data displayed in the F-
View II FW Information window.
Device Name The Device Name relates to the inner construction of the camera and is
specified by the manufacturer.
34
Devices ID The Device ID is the physical identification of the camera for the operating
system. It is in accordance with the device's serial number.
Firmware Version The Firmware is the cameras software for your operation.
Serial Number The serial number serves as a clear identification for each camera
produced.
Camera Type The Camera Type is the exact identification for the camera model.
DLL Version The DLL Version is the version number of the camera's driver.
SYS Version The SYS Version is the version number of the camera's device driver. DLL
and SYS Version are identical.
Temperature The camera is equipped with temperature sensors. The temperatures are
continually measured.
CCD Chip CCD Chip is the CCD chip's temperature. If this temperature is high it will
lead to an increase in noise. The default value for trouble-free operation is
10°C.
Housing Housing is the temperature of the cameras housing.
Display tab
Display tab
Over exposure The Over exposure group enables you to determine if and when you should
be warned of an overexposure. The software continually checks the image
intensity in live mode, by analyzing the image's gray-value histogram in real
time. You will be warned if the live-image is overexposed. The warning will
also appear following acquisition of an overexposed single image.
Display warning Select the Display warning check box to have a warning message shown if
the image is being overexposed:
35
In this case, reduce the exposure time till the warning message disappears.
Overflow An image is overexposed when a certain percentage of its pixels attain the
maximum gray value possible. This percentage is entered into the Overflow
field.
Automatic gain display Use the functions in the Automatic gain display group to have the image
always displayed optimally on-screen, no matter what the actual lighting
conditions are. The system assesses the current gray-value histogram - in
real time - in order to obtain optimal display of the live-image on the monitor.
Please note that the Automatic gain display not only alters the monitor
display, but the actual image information as well. If it is activated, the reduc-
tion of the acquired image from 3 x 12 bit to 3 x 8 bit takes place with the
help of optimized lookup tables. If the Automatic gain display is not acti-
vated, the 4 lower bits (from the 12 bits of each pixel) will be clipped.
Activate Select the Activate check box to enable the Automatic gain display. The
effect of this check box is the same as clicking the Use automatic gain
display button located in the Camera Control dialog box (<). The Activate
check box is not available if the check box with the same name in the Fixed
Scaling group is enabled.
Left overflow When the automatic gain display is active, image intensity for the image on
Right overflow the monitor will be spread between the gray values Gmin and Gmax. You
determine the values of these Gmin and Gmax gray values in the Left over-
flow and Right overflow fields: this is where you enter the percentage of
pixels with the lowest gray values which are to be displayed black, and which
percentage of the highest gray values are to be displayed white.
This is how you clip gray values that are to be ignored at both the upper and
lower ends of the gray-value histogram. Make use of the overflow values to
stop individual pixels or minute image areas that are too bright or too dark
from influencing the Automatic gain display.
Fixed scaling Instead of using the Automatic gain display group, you can use the Fixed
scaling group. The group whose Activate check box has been selected will
be the one available - the other will not be available.
The functions of the Fixed scaling group are for manually influencing on-
screen image display. You select definite limits - Gmin and Gmax - for the
gray-value range that you wish to have optimally spread for on-screen
display. Any gray values occurring outside this defined gray-value range will
appear either black or white.
Application This is how you can, for example, improve the visibility of the specimen
structures of interest to you - by having dark areas of the specimen
displayed entirely in black.
Please note that this manual presetting of the gray value, like the Automatic
gain display, not only effects the monitor display, but also the image infor-
mation.
Activate Select the Activate check box to switch on the manual definition of the gray-
value range to be displayed.
Left limit Enter the value of the left limit for the fixed scaling into the Left limit box.
Right limit Enter the value of the right limit for the fixed scaling in the Right limit box. Its
36
function is equivalent to the Right limit parameter, which you can influence
by clicking the Manually adjust fixed scaling button located in the Camera
Control dialog box.
Display tab
Online histogram Select the Online histogram check box to enable you to keep an eye on the
gray-value distribution during image acquisition. During image acquisition, a
window showing the current histogram will appear automatically. This histo-
gram will be continually updated.
Minimum, mean, and maximum gray values of the image will be shown
beneath the histogram. Percentages are in relation to the maximum obtain-
able gray value. The maximum obtainable gray value when using F-View II
(with its view 12-bit depth) is 4095. This gray value is equivalent to 100%. A
mean value of 19,2% is equivalent to a gray value of 788.
During a live acquisition, you can turn the Automatic Gain Display on or off
with the buttons in the online histogram, as well as activate and set the fixed
scaling.
Setting the fixed scaling You use the mouse to set the limits of the fixed scaling in the online histo-
via the online histogram gram. Move the pointer over the blue (lower limit) or red (upper limit) vertical
line in the histogram. The pointer turns into a horizontal double arrowhead.
Move the mouse with the mouse button depressed to the left or right to either
increase or decrease the limit values. The changes can be viewed directly
in the live-image.
Real time FFT The Real time FFT check box is only available in black & white mode. In
order to be able to use the Real time FFT during live acquisition, you must
select the Live Overlay check box. An explanation of the effects of the
enabled Real time FFT can be found in the section entitled Display and
usage of the acquisition with Real time FFT.
37
Live overlay Select the Live overlay check box if you want to use overlays in live-images.
Live overlays are absolutely necessary for the realtime FFT mode.
An image’s overlay contains information in the form of text, markers, or other
graphical elements. The overlay can be displayed together with the corre-
sponding image without the actual image information being affected. The
live overlay must be enabled in order to make Real time FFT possible.
The Live overlay check box is only available if your display board features a
so-called hardware overlay which is supported by Microsoft DirectX. Almost
any up-to-date AGP display board provides you with that feature. You can
look at the System Info dialog box to check whether your display board
offers this feature. To do so, select the Info... command from the ? menu and
click the System Info... button.The System Info dialog box will be opened.
Click on the "DirectX" entry in the list on the left-hand side to have the
desired information appear in the list on the right-hand side.
Related topics
Automatic Gain Display and fixed scaling 15
Acquisitions with Real time FFT 23
Displaying the histo- Select the Show histogram ROI check box to have the ROI for the histogram
gram ROI displayed during the live mode.
Fixed zoom for binning Select the Fixed zoom for binning check box to increase the view of the
image using binning 4 and 8.In this case, this image and acquired image are
automatically shown with a zoom factor of 200% with binning 4, and a zoom
factor of 400% when using binning 8.Should the Fixed zoom for binning be
activated, the value in the Image scaling field is ignored as soon as binning
4 or binning 8 are activated.
Image scaling Select one of the four selections for displaying an acquired image in a View-
port in the Image scaling list.
Underscan Select the Underscan entry to have the whole image displayed. The system
will select the largest zoom level - 25%, 50% or 100% - where the whole
image can still be displayed within the Viewport. In certain cases not all of
the Viewport’s available area will be used.
Overscan Select the Overscan entry to ensure that all the Viewport’s available area will
be used. The system will select the smallest zoom level - 25%, 50% or 100%
- where the image fills the whole Viewport. In certain cases parts of the
image will not be visible.
Adjust to viewport Select the Adjust to viewport entry to have the image size adjusted to opti-
mally fit the Viewport. The image will be adjusted to fit the current size of the
Viewport.
Full size (100%) Select the Full size (100%) entry to have the image displayed without any
zooming. Only the left upper corner of the image will be shown if the View-
port is smaller than the image.
Histogram calculation Select the pixels to be taken into consideration for calculation of the gray-
value histogram in the Histogram calculation list. There are three options:
Full image If you select the Full image entry, the gray values of all pixels will be applied
to histogram calculation. This is the most commonly used setting when
using light microscopy.
Crosshairs If you select the Crosshair entry, only pixels of a particular image area are
used in the calculation. This image area is shaped like cross hairs that are
centrally positioned on the image. The vertical and horizontal segments of
these cross hairs are respectively just a few pixels wide and cover just about
38
the entire height and width of this image.

ROI Select the ROI entry to carry out the optimization of contrast and exposure
time based on a rectangular frame.
These settings affect all of the real time functions that use the gray-value
histogram: the warning for Over exposure, the Automatic gain display and
the Online histogram.
Application We recommend selecting Crosshair if you wish to speed up histogram
calculation. You can speed up the frame rate of the live-image depending on
what real time functions you have activated. You should however make sure
that the pixel gray values around the middle image line and around the
middle image column are representative for the gray values of the entire
image. If this is the case, you will not notice any difference compared to the
Full image option for the over exposure warning, Automatic gain display,
and the online histogram.
Display palette You can select a false color lookup table from the Display palette list to color
the acquired image.
- none - Select the entry - none -, if you don't want to use any palette at all for the
image display.
Format tab
Adjust, Dither, Gamma, The entries in this list are the names of various lookup tables which can be
Mixmap Rainbow, used for the image display on the monitor.
Solaris, Thermal
Format tab
Active area The display in the Active area field shows how large the acquired image
segment is compared to the largest possible image, when the current
settings are applied. If you make changes to the clipping, you can directly
view their effect here on the active area.
Image format The entries in the Image format list show formats which are realized by using
binning and clipping. The entries of the list show the corresponding binning.
39
You can see the clipping used displayed in the Active area field. The entries
in this list correspond to predetermined settings for the clipping. In addition
to the predetermined list elements, you can also select from the settings you
have defined.
Binning In the Binning group you can set the binning factor (1x, 2x, 4x, 8x). The fields
Horizontal and Vertical are coupled. When changing the value in one of the
fields, the value of the other field is changed accordingly.
Clipping border (pixel) When clipping is used, the edges of the images are cut off. Hence, clipping
does not have any effects on the resolution and brightness nor on the
required exposure time.
Left, Right, Top, The fields entitled Left, Right, Top and Bottom show the number of pixels
Bottom that are to be clipped from each image edge. Each individual value can
range from 0 (no clipping) to almost the entire image in each respective
direction (complete clipping). If a value for the clipping has already been set,
the setting possibilities for clipping the opposite edge reduce themselves
respectively.
Interactive Click the Interactive button to be able to place the clipping interactively in the
image document. Move the pointer with the mouse button depressed to
change the size of the area to be acquired. The currently acquired area will
be indicated by a red frame. Move the mouse to alter the position of the
marked area within the image document. Rightclick once to fix the area to
be acquired.
No Clipping Click the No Clipping button to return the values for Left, Right, Top and
Bottom to 0, thus disabling Clipping.
40
Appendix I: Microscope settings
F-View II
Appendix I: Microscope settings

Your digital camera can only achieve high image quality if the microscope
has been optimally set. A subsequent correction to the software can never
correct deficiencies in the image resulting from faulty settings made to the
camera and microscope.
The optical system microscope offers numerous setting possibilities. In
order to attain best acquisition results, the settings must be made precisely.
In spite of varying models and observation possibilities, the microscope's
image producing components are in accordance with each other.
A microscope basically consists of the optical components: projection objec-
tive and magnifier. The projection objective is the objective with which the
observation is to be carried out. The eyepiece takes on the function of the
magnifying glass. An additionally important component is the illuminator.
This illustration clarifies the
basic assembly of the Kamera
camera
components of a micro-
scope in transmitted-light Lupe
Lens
mode.
Okular
ocular
Objektiv
objective Projektion
Projection
Probe
specimen
Kondensor
condenser
Beleuchtung
Illumination
Lampe
lamp
41
Illumination
Light is a decisive medium for creating images with a microscope. As a result, the light, i.e. illumination,
plays an important role in creating the image in the microscope.
The goal of the illumination settings is the equal distribution of light on the
sample with parallel light rays falling on the object. The illumination must be
bright enough to make all of the image details visible; however over-illumi-
nation leads to images being too bright and low-contrast. In addition, the
color of the light has a strong impact on the sample's color rendering.
Lamp Selection The tungsten-halogen lamps and gas discharge lamps are the most
commonly used lamps in microscopy. The light creating processes of both
lamp types are fundamentally different.
Tungsten-halogen Tungsten-halogen lamps comply with their functionality to a conventional
lamps light-bulb. Electricity flowing through a metal wire heats the wire to the extent
that light is produced (thermal emitter). In doing so, the wire emits a contin-
uous spectrum, hence a broad spectral band is emitted. One can control the
F-View II • Illumination
brightness as well as the color (color temperature) of the lamp by controlling

the electrical current. Tungsten-halogen lamps can be widely used in
microscopy, especially when acquiring color images.
Gas discharge lamp Gas discharge lamps stimulate gas at the atomic level using strong electrical
fields. The excited atoms return to their initial state and, in doing so, give off
the energy which initially charged them in the form of electromagnetic radi-
ation: light. The resulting photons each have characteristic energy which is
typical for the gas being used. As a result, this type of lamp does not emit a
broadband spectrum like the halogen lamp, but instead emits discrete wave-
lengths. This fundamental physical process cannot be externally influenced;
hence, such a lamp's perceived color is independent of the operation condi-
tions. Because of this discrete spectrum, gas discharge lamps do not emit a
color-neutral illumination. Its adjustable high intensity light and the emission
of short-wave spectral bands required especially for fluorescence micros-
copy is an advantage.
ND filter Many microscopes can insert ND filters into the light path, thus reducing the
intensity of the lamp's light. These ND filters ensure a uniform reduction of
light intensity throughout the entire frequency range without altering the
color temperature.
Köhler Illumination
This illumination process which was described by Dr. August Köhler as early
as 1893, is still an indispensable prerequisite for successful analysis using
light microscopy. The Köhler illumination enables an optimal, homogenous
illumination of solely the part of the sample to be analyzed.
Condenser The condenser carries the light onto the sample. The main components are
the aperture stop with which the size of the illuminated area is set and the
condenser lens which is responsible for the parallel emitted light rays. The
condenser can be opened and closed. The condenser is height-adjustable
and horizontally adjustable.
Note Set the aperture stop to 2/3 of the entire opening and do not use it to reduce
the brightness. To do that, you should always use ND filters or a lower lamp
voltage.
42
Principle of Köhler Illumi- will find comprehensive descriptions about the principle of Köhler illumina-
nation tion in microscopy text books. We will only give you a brief overview about
some important settings you should know.
Focussing the Field One vital step when setting up the Köhler illumination is the sharp view of
Diaphragm the field diaphragm at the specimen level.
1) Focus the object to be viewed.
2) Close the field diaphragm so that only a small bright spot is visible in
the eyepiece.
3) Focus the image in the field diaphragm by using the condenser's height
adjustment.
Adjusting the Now the illuminated area is optimally set.
Condenser 1) Open the field diaphragm so that its edge is still completely within the
visible area.
2) Center the image of the field diaphragm using the condenser's adjust-
ment screws.
" A precisely adjusted condenser guarantees that only the area of the
specimen to be viewed is illuminated.
Objective, Total Magnification and Useful Magnification
3) Repeat steps 1) and 2), until the edge of the field diaphragm is no
longer visible in the visual field.
Adjusting the Lamp You can generally skip adjusting the lamp with modern microscopes,
provided that the lamp is located in a frame and the bulb is in a centered
socket.
Objective, Total Magnification and Useful Magnification

There are no general rules for which objective you select; one should always
keep the specimen and reason for observation in mind.
Terminology The two most important parameters of an objective (magnification and
numerical aperture) are engraved in the objective. First the magnification
and then the numerical aperture which is separated by a diagonal slash.
Hence, the code 40 / 0,65 marks an objective with a magnification of 40X
and the numerical aperture of 0.65.
Magnification An objective's engraved magnification is considerably important for the total
magnification. However, one should note that the range of useful magnifica-
tion should not be surpassed.
Numerical Aperture The numerical aperture is the characteristic of an objective which deter-
mines its capacity. The numerical aperture can also be found engraved into
the objective. It is based on the refraction index of the lenses being used as
well as the objective's angular aperture. The manufacturers are narrowly
limited when dealing with the refraction index, whereas the objective's
angular aperture can be increased by using appropriate measures. To
achieve this, a fluid with a high refraction index is placed between the cover
slip and the objective. The immersion objectives made for this technique are
marked accordingly. They should be used with the appropriate immersion
fluid only.
Total magnification The system's total magnification, to which an installed TV adaptor contrib-
utes, is based on the actual size of the final image. This can be the image
on the monitor, as well as the image printed on paper. To determine the total
magnification, it is best to use a stage micrometer which can be shown on
the monitor or the printout. You can determine the total magnification by
dividing the length of the object shown by the set length on the micrometer.
43
Useful magnification You can determine the total magnification by dividing the length of the object
shown by the set length on the micrometer. The useful magnification of the
optical systems microscope depends on the numerical aperture being used
( engraved on the objective). The useful magnification is in the range of 500x
to 1,000x the numerical aperture.
Empty magnification A loss of contrast and sharpness is the result if total magnification is larger
than the useful magnification. Check to see if replacing the TV adapter with
a 0,5 or 0,63 model sufficiently reduces the total magnification. If this is not
possible, you can also use an objective with the same magnification, but
with a higher numerical aperture.
F-View II • Appendix II: How to install the camera
Appendix II: How to install the camera

Warning Please read the following carefully before attempting installation of
camera.
Requirements
The F-View II can be used under the operating systems Windows 2000 and
Windows XP. The installation procedure is described separately for these
two operating systems.
Installation sequence In order to avoid problems, you’ll have to follow the steps of the procedure
in the order given here.
Warning Install the image-analysis software first before connecting the camera
Windows 2000 to the FireWire board.
1) Check whether your computer system has a FireWire interface

onboard or a FireWire board installed.
2) Install the FireWire board provided with the camera.
3) Install the image-analysis software on your computer.
4) Install the camera and connect it to the computer system.
What if my computer If your computer system already has a FireWire interface onboard or a sepa-
system already has a rate FireWire board installed, you do not need to install the board provided
FireWire interface? with the camera. If you are not sure whether a FireWire interface is available,
open the Windows Device Manager via Start > Settings > Control Panel >
System > Hardware > Device Manager....
44
An entry called ’IEEE 1394 Bus host controllers’ shows that the needed
FireWire interface is available.
General Warnings!
The FireWire board IEEE-1394
• CCD image sensors are easily damaged by static discharge. Do not

disassemble the camera housing.
• Do not attempt to touch the internals of the camera with your bare
hands.
• Do not touch cover slip (over the CCD chip) with your hand or have any
object come in contact with glass surface. Should dust stick to the
cover slip, blow it off gently with an air blower. (For dust stuck due to
static electricity, ionized air is recommended.)
• It is best to use a LensPen to clean the cover slip. Do this only when
absolutely necessary.
• Do not expose to strong light (sunlight) for long periods.
• Exposure to high temperature or humidity will affect the characteristics
of the camera. Avoid storage or usage under such conditions.
• Do not subject the camera to too much mechanical shock.
• The FireWire cable plug is not symmetrical. You must make sure that
the cable plug is facing the same way as the camera socket when you
plug the cable into the camera jack.
• Do not disconnect the FireWire cable while the image-analysis soft-
ware is running.
The FireWire board IEEE-1394

Warning! • The components of these devices are easily damaged by static
discharge. Wear grounding wrist straps when handling them.
• Do not plug in or unplug the cards from the PCI slot while the PC is on.
• Please follow the installation manual in this booklet step by step to
avoid any incorrect connections. Incorrect connections may result in
serious damage to your PC system and/or the camera.
Installation Procedure - Windows 2000
45
Installation of the FireWire board IEEE-1394
When is this necessary? This procedure is necessary only if the PC does not have a FireWire inter-
face on-board hardware configuration or if it is not yet equipped with a
FireWire board.
F-View II • Installation Procedure - Windows 2000
The FireWire board
How to install the 1) Turn off the PC and disconnect the power supply.
FireWire board 2) Remove the cover from the PC.
3) One PCI slot is required for the FireWire board. Insert the board into the
slot.
FireWire board installed
46
4) Replace the cover onto the PC.

5) You are now ready to first install the image-analysis software and then
the camera.
Installation of the image-analysis software

Warning! The image-analysis software must be installed first before connecting the
camera to the FireWire board.
Requirements, PC The F-View II camera requires the image-analysis software, (3.2 version),
and Microsoft Windows 2000 (or later).
camera driver installation Installation of the camera driver is simply done via the image-analysis soft-
ware setup. You are only required to select the camera during setup.
Installation of the image- For installation, follow the instructions given on the monitor. You do not have
analysis software to select a digitizer in the Input Device (Digitizer) Selection dialog box.
Select Olympus Soft
Imaging Solutions as manu-
facturer and F-View II as
camera
.In case you work with the
trigger model of the F-
View II, select the entry
F-View II Trigger instead.
Precalibration You will be advised to carry out a precalibration of the camera when
installing the image-analysis software.
In this dialog box you can
activate the precalibration.
47
Select the TV adaptor's magnification from the list which has the same
name. Should you not be working with a remote microscope, clear the check
box. Then, mark the checkbox for all mounted objectives in the Magnifica-
tion of the microscope objectives list.
Note Should you use objectives with a magnification that does not appear in the
list, a manual calibration must be carried out for these objectives. A guide is
part of the image analysis system's step by step guide.
Note The image-analysis software can support several same or different types of
FireWire cameras simultaneously. You differentiate F-View II view cameras
via their serial numbers when creating a new logical input channel via Image
> Set Input... > New Channel. A camera’s serial number is located directly
below the FireWire jack on the camera housing.
The camera’s serial number
is located below the
FireWire jack.
48
Installation of the camera and cables
Installation of the camera and cables

Don’t forget... Install the image-analysis software first before connecting the camera to the
FireWire interface.
How to install the camera 1) The diagram shows a typical installation of a light microscope. You
should have all required components ready at hand.
FireWire-cable
F-View II
C-Mount adaptor
PC with image analysis progr

and FireWire-port
49
FireWire-plug
2) Remove the protective cap from the camera.

3) Firmly screw the TV adaptor (0,5x or bigger") onto the camera. This
should be done with the CCD-chip side of the camera facing down to
minimize the chance of dust landing on its surface.
4) Install this assembly onto the microscope's camera mount. Some
microscopes may require additional optical elements installed between
the camera mount and the C-mount adapter. You may also need to
align the orientation of the microscope with that of the stage, and also
match the focus of the camera image with that of the eyepiece image.
These settings can be done later when you are able to obtain an image
on the screen.
5) Plug one end of the FireWire cable into the camera connector.
" The FireWire cable plug is not symmetrical. You must make sure
that the cable plug is facing the same way as the camera socket
when you plug the cable into the camera jack. The camera chip
may be ruined otherwise.
Make sure the cable plug is
facing the right way before
you plug it into the camera
socket
6) Plug the other end into one of the small connector sockets on the
FireWire board.
" There are two or more jacks depending on the FireWire board. You
can use any of them.
" The Digital Signature Not Found MS Windows dialog box(es)
appear on your screen. Confirm the message as often as neces-
sary until the dialog boxes disappear.
50
7) Open the Windows Device Manager via Start > Settings > Control
Panel > System > Hardware > Device Manager....
" The Device Manager dialog box contains the correct camera driver
entry Olympus Soft Imaging Solutions.2.1.2 (SoftHard). The
camera is ready for use.
Troubleshooting
The Windows Device Manager shows the IEEE 1394 FireWire board and the camera Olympus Soft Imaging Solutions
FireWire Camera 2.1.2 (SoftHard).
Troubleshooting
What do I do if the An error message appears when starting the program, even though you
camera cannot be have completely installed the camera and the image-analysis software:
found?
51
The image-analysis software has not been successful in creating a connec-
tion to the camera. Confirm the message with OK.
Check to see if the connector cable between the camera and the computer
has been correctly plugged-in. Should the connecter be loose or not plugged
in correctly, correct this by firmly plugging them into the corresponding jack.
Restart the image-analysis software. Should the problem still exist, continue
with the following section.
What to do when the The error message No SIS-View FireWire camera has been found will also
camera has been appear when starting the program should the camera have been connected
connected before the before installing the image-analysis software. In this case, Windows 2000
image-analysis software has already installed its own camera driver, which prevents the error-free
installation
communication between the camera and the computer. Installing the image-
analysis software now does not install the correct camera driver since this is
forbidden by Windows 2000.
If the camera is hooked up to the computer, you have to install the correct
camera driver from the software’s setup CD separately from the software
installation. Do the following:
1) Select the Start > Settings > Control Panel > System > Hardware >
Hardware Wizard... command.
2) Click on the Next button.

52
Troubleshooting
3) Select the Add/Troubleshoot a device option and click on the Next

button.
53
4) Select the entry Olympus Soft Imaging Solutions FireWire Camera

2.1.2 (SoftHard) from the Devices list and click the Next > button.
5) Click on the Finish button.

54

Troubleshooting
7) Select the Search for a suitable driver for my device (recommended)

option and click on the Next button.
55
8) Insert the image-analysis software setup CD into your computer’s CD-

ROM drive. Minimize the appearing setup dialog box.
9) Make sure that only the CD-ROM drives check box is selected and click
on the Next button.
" Wait until Windows has found the correct camera driver. The
driver's data file is mm282osis.inf. You will find it on the CD-ROM
in Directory\program\support\cameras\sis_firewire\drivers\win2k.

" The Digital Signature Not Found dialog box appears on your
screen.
56
11) Click Yes.

" Wait until the camera driver has been installed.
Troubleshooting
12) Click on the Finish button.

" The Digital Signature Not Found MS Windows dialog box(es)
appear on your screen. Confirm the message as often as neces-
sary until the dialog boxes disappear.
57
13) Open the Windows Device Manager via Start > Settings > Control
Panel > System > Hardware > Device Manager....
" The Device Manager dialog box contains the correct camera driver
entry Olympus Soft Imaging Solutions.2.1.2 (SoftHard).
The Windows Device Manager shows the IEEE 1394 FireWire board and the camera Olympus Soft Imaging Solutions
FireWire Camera 2.1.2 (SoftHard).
14) Install the image-analysis software. During the installation check the
Firewire check box to in the Input Device (Digitizer) Selection dialog
box. Select the F-View II check box in the Camera Selection dialog box.
58
Installation Procedure - Windows XP
Installation Procedure - Windows XP

Installation of the FireWire board IEEE-1394
When is this necessary? This procedure is necessary only if the PC does not have a FireWire inter-
face on-board hardware configuration or if it is not yet equipped with a
FireWire board.
The FireWire board
How to install the 1) Turn off the PC and disconnect the power supply.
FireWire board 2) Remove the cover from the PC.
3) One PCI slot is required for the FireWire board. Insert the board into the
slot.
FireWire board installed
59
4) Replace the cover onto the PC.

5) You are now ready to first install the image-analysis software and then
the camera.
F-View II • Installation Procedure - Windows XP

Warning Install the image-analysis software first before connecting the camera to the
FireWire interface.
Requirements, PC The F-View II camera requires the 625 image-analysis software and
Microsoft Windows XP (or higher). The image-analysis software also
supports multithreading on multiprocessors, thus making full use of the
power of the latest multi-CPU PC’s.
camera driver installation Installation of the camera driver is simply done via the image-analysis soft-
ware setup. You are required to select FireWire and specify the additional
camera driver during setup. Then you have to get Windows to use the
camera driver.
Installation of the image- For installation, follow the instructions given on the monitor. You do not have
analysis software to select a digitizer in the Input Device (Digitizer) Selection dialog box.
Select Olympus Soft
Imaging Solutions as manu-
facturer and F-View II as
camera
.In case you work with the
trigger model of the F-
View II, select the entry
F-View II Trigger instead.
60
Verwandte Themen
Installation of the camera driver, S. 64
Precalibration You will be advised to carry out a precalibration of the camera when
installing the image-analysis software.
In this dialog box you can
activate the precalibration.
Select the TV adaptor's magnification from the list which has the same
name. Should you not be working with a remote microscope, clear the check
box. Then, mark the checkbox for all mounted objectives in the Magnifica-
tion of the microscope objectives list.
Note Should you use objectives with a magnification that does not appear in the
list, a manual calibration must be carried out for these objectives. A guide is
part of the image analysis system's step by step guide.
Note The image-analysis software can support several same or different types of
FireWire cameras simultaneously. You differentiate F-View II view cameras
via their serial numbers when creating a new logical input channel via Image
61
> Set Input... > New Channel. A camera’s serial number is located directly
below the FireWire jack on the camera housing.
The camera’s serial number

is located below the
FireWire jack.
How to install the camera 1) The diagram shows a typical installation of a light microscope. You
should have all required components ready at hand.
FireWire-cable
F-View II
62
C-Mount adaptor
PC with image analysis program

and FireWire-port
FireWire-plug
2) Remove the protective cap from the camera.

3) Firmly screw the TV adaptor (1/1,8" or bigger) onto the camera. This
should be done with the CCD-chip side of the camera facing down to
minimize the chance of dust landing on its surface.
4) Install this assembly onto the microscope's camera mount. Some
microscopes may require additional optical elements installed between
the camera mount and the C-mount adapter. You may also need to
align the orientation of the microscope with that of the stage, and also
match the focus of the camera image with that of the eyepiece image.
These settings can be done later when you are able to obtain an image
on the screen.
5) Plug one end of the FireWire cable into the camera connector.
" The FireWire cable plug is not symmetrical. You must make sure
that the cable plug is facing the same way as the camera socket
when you plug the cable into the camera jack. The camera chip
may be ruined otherwise.
Make sure the cable plug is
facing the right way before
you plug it into the camera
socket
6) Plug the other end into one of the small connector sockets on the
FireWire board.
" There are two or more jacks depending on the FireWire board. You
can use any of them.
7) Continue with the camera driver installation before starting the image-
63
analysis software.
Installation of the camera driver

The camera driver is not signed and therefore not used automatically under
Windows XP. After having installed the image-analysis software and
connected the camera to the computer - or vice versa - you have to go
through the following steps to get Windows XP to use the camera driver:
1) Open the Windows Device Manager via Start > My Computer > (right
mouse) > Properties > Hardware > Device Manager....
64
" The Device Manager dialog box shows the Generic 1394 Desktop
Camera entry in the Imaging devices section. The exclamation
mark indicates a device conflict.
2) Select the Generic 1394 Desktop Camera entry and click on the
Update driver button in the device manager button bar.
3) Select the option Install from a list or specific location (Advanced) and
click the Next > button.
65
4) Select the Don’t search. I will choose the driver to install. option and
click on the Next button.
" The Model may already contain one ore more entries based on
previous software installations.
5) Select the entry Olympus Soft Imaging Solutions FireWire Camera
2.1.2 (SoftHard) or higher.
" If this entry is missing, click on the Cancel button. Repeat the instal-
lation of the image analysis program including the selection of F-
View II as camera.
" Do not select an entry reading OLYMPUS CORPORATION
FireWire Camera.
" The next Hardware Update Wizard dialog box will be opened in the
background displaying the message to wait while the Wizard
66
installs the software.

" Additionally, the Hardware Installation dialog box will pop up in the
foreground.
Verwandte Themen
Installation of the image-analysis software, S. 60
7) Click on the Continue Anyway button.

" The Hardware Update Wizard dialog box which had been in the
background, will become visible.
67
" After having finished the camera driver installation the following
dialog box will be opened.
8) Click on the Finish button to end the hardware update wizard.

" In the Device Manager dialog box there is still the same Generic
1394 Desktop Camera entry but the exclamation mark indicating
the device conflict should have disappeared. The camera is now
ready for use.
68
In case of problems wih the camera
In case of problems wih the camera

Should you have questions or should problems arise which you cannot solve
with the help of the documentation provided, please feel free to contact our
customer service - preferably via E-mail. Our customer-service personnel
will be more than happy to assist you.
1) Please clarify before hand when and under what circumstances the
problem arose.
• It would be best if you can clearly reproduce the problem/error. You
will therefore make it easier for you and our customer-service
personnel to solve the problem.
2) Please write down the exact wording of the (error) messages which
appear.
3) As an alternative, you can make "Snapshots" of these messages. You
can make a snapshot of your active window by using the key combina-
tion [Alt+Print]. A copy of the active window is stored to your clipboard
and can be easily attached to an E-mail using [Ctrl+V].
4) Since very large E-mails can create transmission problems both for the
sender and the receiver, do not copy "screenshots" of the entire
contents of the screen. For the same reasons it is advisable to reduce
the color depth of the snapshots.
5) Should the camera basically work, but problems with image quality
arise, save an image in which the problems are clearly visible in TIF
format. Insert this example image in your E-mail to our customer
service.
6) Start the image-analysis software.
7) Select the ? > Info... command to open the Info dialog box.
" The Info dialog box shows you information about the software
package (Docu, Auto, Pro) of your image analysis program, the
serial number of your image analysis program and the operating
system being used.
• The software data mentioned should be at hand when contacting
our customer service personnel.
8) Please write an E-mail clearly describing the problem (including snap-
69
shots and example images), as well as the software data from the Info
dialog box to our customer service address.
support@olympus-sis.com
• The quickest and most convenient way is to use the automatic E-

mail settings for your customer service query:
By using the ? > Info... > System Info... > Send button, an automatic
E-mail is created which you can then complete and send to us. If
you have made an example in TIF format, please attach this to the
E-mail as well. Before sending, read the notes in your E-mail box
the data about your system which you will send to us in this E-mail.
Should you not be able to send E-mails from your computer, use
the? > Info... > System Info... > Info save save button to save the
files and to send them from another computer.
• You may of course contact us via telephone or fax>
Tel.: (+ 49) 2 51 / 7 98 00-6444
Fax: (+ 49) 2 51 / 7 98 00-6060
WEEE DECLARATION
Waste Electrical In accordance with European Directive 2002/96/EC on Waste Electri-
and Electronic cal and Electronic Equipment, this symbol indicates that the product
Equipment must not be disposed of as unsorted municipal waste, but should be
collected separately. Refer to your local distributor for return.
CE DECLARATION OF CONFORMITY
Product Type CCD Camera

Brand Name Soft Imaging System
Model Designation F-View II
Name and Address of manufacturer or representative
Manufacturer Soft Imaging System GmbH

Johann-Krane-Weg 39
D-48147 Münster
The product complies with the requirements of the following European directives:
89/336/EWG Directive of the council on the approximation of the laws of Member

States relating to electromagnetic compatibility changed by directive
91/263/ECC, 92/31/ECC and 93/68/ECC of the council.
Compliance was proved by the application of the following
electromagnetic compatibility standards:
EN 55022 : 1998+A12000, cl. B
EN 55024 : 1998
EN 50081-1 : 1992
EN 50082-1 : 1997
EN 61000-4-2
EN 61000-4-3
EN 61000-4-4
EN 61000-4-6
ENV 50204
Date: April 30th, 2002
Dr Manfred Schierjott April 30th, 2002

Date
Index
Index
G
A Gamma Correction
RGB mode 16
analySIS, version 5
Automatic Gain Display
Input 35 H
Housing temperature 34
B
Binning 39 I
Image scaling, Display tab 38
C intelligent Exposure
Calibration 9
Camera configuration 31 Exposure Time Lock 10
Camera Control Online shading correction 22
Camera Type 34
Clipping 39 K
Color Settings
Gamma Correction 16 Köhler illumination, microscope 42
Configure Input 32
L
D Live overlay 37
Device ID 34
Device Information 34
Display tab M
Image scaling 38 Microscope 41
Over exposure 35 Empty magnification 43
72
Palette 38 Illumination 41
DLL version, F-View II III information 34 Numerical Aperture 43
Duplicate Channels 32 Objective 43
Total magnification 43
Useful magnification 43
E Microscope Settings 41
Enabling Shading Correction 20 Mirror 33
Exposure lock, Intelligent Exposure 10
Exposure time
Input 33 N
NetCam 26
in the internet 27
F In the intranet 27
Firmware Version 34 Settings 29
F-View II Information 34 New Channel 31
Index
O U
Online histogram 37 Useful magnification, Microscope 43
Online shading correction 17
intelligent Exposure 22
Over exposure 35 W
Over Exposure Warning 35
Web Server 27
P
PC, Requirements 5
R
Real time FFT 37
Requirements, PC 5
RTFFT
Control 23
Display 24
S
Select device 32
Serial Number 69
Serial number 34
Set Input 31
Shading Correction
Activate 20
System Info 69
73
T
Tab Format
Binning 39
Clipping 39
Image format 39
Tab Input
Automatic gain display 35
Exposure time 33
Info 34
Mirror 33
Temperature 34
Tools 25

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Manual

© Olympus Soft Imaging Solutions GmbH

The F-View II - camera 4

WHEN CAN THE IMAGE BE TOO DARK? ..............................7

Acquiring images with manual control 12

OPTIONS IN THE CAMERA CONTROL ................................15

Online shading correction 17

USING THE SHADING CORRECTION ..................................18

Acquisitions with Real time FFT 23

INSTALLING THE WEB SERVER ..........................................27

NETCAM IN THE INTRANET ................................................. 27

Setting up the camera 31

LOGICAL INPUT CHANNELS ................................................ 31

Appendix I: Microscope settings 41

Appendix II: How to install the camera 44

INSTALLATION PROCEDURE - WINDOWS 2000 ................ 45

In case of problems wih the camera 69

The F-View II - camera

Overview of the chapters

2) Click the Acquire > button.

When can the image be too dark?

The lower part of the image

2) Click the Set ROI for White Balance button.

When the ROI for the calcu-

With the default setting of

2) Move the Exposure adjustment slide control to the right.

Under the same conditions,

Calibrating Intelligent Exposure

The second calibration step consists of measuring the signals at various

Exposure Time Lock

side of the histogram will be

Online histogram with

Acquiring images with manual control

Preparing the input channel

Options in the Camera Control

Button Area Effect

If you start a live acquisition with an activated Sharpness Monitor or activate

This is because there is no absolute measurement for the sharpness in an

Using the sharpness monitor

Online shading correction

Using the Shading Correction

How the Shading Correction functions

have to change the Online Shading-Correction's setting.

Acquiring Reference Images

Enabling Shading Correction

Repeat or Update the Reference Acquisitions

Use of the Online Shading Correction

that a missing image is required and automatically requires you to reacquire

Intelligent Exposure and Online Shading Correction

Acquisitions with Real time FFT

The Tools tab offers RTFFT-related tools.

Installing the web server

You can simply adopt the default settings.

NetCam in the intranet

NetCam in the internet

Determining the IP address

3) Note the entry in the IP address row.

Setting up the camera

Select Device dialog box

The Configure Input dialog box

the entire height and width of this image.

Appendix I: Microscope settings

brightness as well as the color (color temperature) of the lamp by controlling