Keywords:

Gas chromatography–mass spectrometry (GC–MS)

inductively coupled plasma (ICP)-MS
selenocysteine (SeCys)

Selenomethionine (SeMet)

Commonly used compounds can be in the organic form (selenomethionine) or in the inorganic form (sodium
selenite)

liquid chromatography-inductively coupled plasma mass spectrometry (ICP-MS)

gas chromatography (GC) with flame ionization detection (FID)

GC-isotope dilution
MS; GC–ICP-MS; GC with atomic emission
detection, headspace GC with atomic emission detection; liquid chromatography–electrospray tandem MS;
GC with flame photometric detection and gas
chromatography–mass spectrometry (GC–MS)

ethyl chloroformate (ECF)

methyl chloroformate (MCF)

menthyl chloroformate (MenCF),

various seleno amino acids (selenomethione,

selenoethione and selenocysteine) and some sulphur-containing amino acids (methionine, cysteine,
cystine and methylcysteine)
which were included in the experiments for comparison.

selenoamino acid derivatives was accomplished using a Hewlett-Packard HP 6890 gas chromatograph with an
HP 5973 mass selective detector (MSD)

The MSD was operated with electron impact (EI) ionization.

The selected-ion mode (SIM)

The limit of detection (LOD)

gas chromatography–atomic emission detection (GC–AED

dimethyl diselenide (DMDSe),

Gas chromatography(GC)

on-column injection (OCI)

flame ionisation detector (FID),

mass spectrometer (MS, HP 5989A MS Engine, electron impact ionisation)

atomic emission detector (AED