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2.4. Antioxidation effect on oils To compare the anti-oxidation effect of COS-VCE on oils, we added COS-VCE, BHT and a-Tocopherol to cotton seed oil and soybean oil and measured the deterioration of the peroxide value. The result was that COS-VCE showed a better anti-oxidation effect on cotton seed and soybean oil vs. a-Tocopherol. Table 4. Change of the per-oxidation Seed oil to 100°C Oven (ma/kg) ‘Addition — of ‘Afier addition time(hr) Nolume (%) 9 12 15 18 control 342 BS 71.6 100.3 COS-VCE 0.005 26.5 41.0 58.5 85.5 0.02 25.0 37.5 53.8 77.3 BHT 0.02 23.0 32.7 447 613 ‘a-tocopherol | 0.02 29.5 55.4 79.2 116.1 | Table 5. Soybean oil and measured the deterioration of the peroxide value after 23 time to 100°C Oven (maka) Addition — of ‘After addition time (hr) ‘Absorbance Volume (%) 6 9 12 15 Control 248 a7_| 730 [1139 | 0.109 COS-VCE 002 18.9 311 | 485 | 771 | 0260 0.06 188 317 | 486 | 757 | 0.093 BHT (0.02 12.2 225 | 365 | 567 | 0423 o-tocopherol 0.02 25.6 489 | 77.4 | 91.2 | 0.092 2.8. Antioxidation Effect for Two Phase System(water/oil) After mixing of0.5%ofCOS-VCE in water and soybean oil, we blew air onto the oil phase at 100°C to oxidize and measured the changes in peroxide deterioration value. Results showed that COS-VCE inhibited the increase of peroxide value of the oil layer in a two phase system better than control Table 6. Antioxidation Effect for Two Phase System (Water/oil) (maqikg) ‘Addition of Volume ‘After addition time (hr) (Water)-(%) 3 6 9 72 Control 16.4 40.9 79.4 135.6 (COS-VCE 05% 11.7 25.9 40.0 73.6 2.6. Antioxidation effect in mixtures of linoleic acid ethyl ester We mixed the cream of 15% of linoleic acid ethyl ester with COS-VCE and its concentration was 0, 0.1, 0.5, 1.0, 3.0 and 5.0% respectively. We then forcefully oxidized the formulas at 60°C to measure the peroxide value in accordance to the TBA method. Results showed that COS-VCE at concentrations greater than 05% had an antioxidant effect.

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