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Journal of Pharmacognosy and Phytochemistry 2018; 7(2): 2313-2318

E-ISSN: 2278-4136
P-ISSN: 2349-8234
JPP 2018; 7(2): 2313-2318 On vegetative propagation through stem cuttings
Received: 17-01-2018
Accepted: 18-02-2018 in medicinally lucrative Tinospora species
Rakshe Abhijeet
Department of Botany, Rakshe Abhijeet and Digambar Mokat
Savitribai Phule Pune,
University, Ganeshkhind Road, Abstract
Pune, Maharashtra, India
Tinospora cordifolia (Willd.) Miers and Tinospora sinensis (Lour.) Merr. Belong to family
Menispermaceae and known by different common names such as ‘Guduchi’, ‘Gulvel’ and ‘Giloy’. The
Digambar Mokat
Department of Botany, genus has plethora of health benefits described in ancient scripts and traditional systems of medicine
Savitribai Phule Pune, especially its stem. The drug Guduchi is the major ingredients of about 68 Ayurvedic formulations and
University, Ganeshkhind Road, demand of this drug has been increased up to 2000 to 5000 MT with 9.1% annual growth rate. Nevertheless,
Pune, Maharashtra, India natural populations of both the species are dwindling due to indiscriminate harvesting. The huge surge in
demand provides ample scope for scaling up the commercial cultivation of these plants. In the present
investigation, propagation through stem cuttings was studied for developing protocol pertaining to mass
multiplication. Effect of different concentrations of IBA on stem cuttings after 30 DAP and 45 DAP was
studied using design RBD with four replications for both these species. The stem cuttings of T. cordifolia
treated with 100 ppm IBA (T1) exhibited significant rooting percentage i. e. 83.75±3.75a. However, in T.
sinensis, 63.75±2.39a per cent rooting was recorded in treatment control (T0) after 45 DAP. The maximum
and minimum shoot lengths were recorded in treatment T1 (104.15±7.84 cm.) and T0 (48.159±15.58 cm.)
respectively and the maximum numbers of roots (4.25±0.25) and root length (15.42±1.09 cm) was slightly
higher in treatment T1 but significant differences in mean value were not recorded. The average minimum
and maximum growth speed was reported to be 2.33 cm/day to 5.1 cm/day respectively in T. cordifolia
cuttings during the present investigation. In T. sinensis, the maximum rooting percentage was recorded in
control T0 (63.75±2.39) followed by treatment T1 (56.25±8.26). Increased rooting (16.25%) was observed
in 30 DAP to 45 DAP. The numbers of sprouted shoots was higher in control T0 (1.72±0.11). The maximum
numbers of roots was recorded in control T0 (5.5±0.48a). The fresh root biomass (0.847±0.00 g.) and dry
root biomass (0.290±0.00 g.) were found significant in control T0 (Table 4).

Keywords: Tinospora cordifolia, Tinospora sinensis, propagation

The man’s quest for exploration his natural surrounding for drugs has old tradition and history
(Kelly, 2009, Rakshe and Mokat, 2016) [1, 6]. The Indian classic Ayurveda describes a vivid and
detailed account of the medicinal knowledge and practices which were in vogue about 2500
years ago. The therapeutic properties of plants have been used for treating plethora of diseases
under this oldest yet surviving branch of medicine. The global acceptance of our traditional
system is gaining prominence thereby registering steep rise in demand for various plants with
medical properties. India is bestowed with enormous biodiversity of astonishing medicinal
plants having applications and reference not only to Ayurveda but also for Unani, Siddha and
Homeopathy (Pal and Shukla, 2003) [3]. About 47,513 plant species included 18,117 flowering
plants comprising of more than 6,198 medicinal plants have been been documented in India
(ENVIS, 2016, Arisdason and Lakshminarasimhan, 2016, Kavita et al. 2016) [4-6].
The Plant Tinospora is one of the most important plant drug popularly known as ‘Guduchi or
Amrita” and has a wide-ranging bioactive constituents. It has been verified medicinally
important plant by traditional system as well as modern system of medicine. Nevertheless, this
plant not received considerable scientific attention and more investigation especially its
propagation are urgently needed. The drug Tinospora is the major ingredients in about 68
Ayurvedic formulations like ‘Amritharishtam’, ‘Amrithadienna’, ‘Amrithadichoornam’,
‘Dhanvantaram tailam’, ‘Cheriya rasnadi Kashayam’, ‘Valiya marmagulika’, etc. (Sereena and
Remashree, 2014). The innumerable properties of Tinospora drug are described in ancient
scripts of Ayurveda, like Rasayana, Agnideepana, Prameha, Jwarhara, Krimihara,
Correspondence Tridoshshamaka, Dahnashaka, etc. and also confirmed its scientific validity through modern
Digambar Mokat day research (Upadhyay et al. 2010) [8]. The T. cordifolia and T. sinensis are both have been
Department of Botany, recommended in traditional systems of medicine from prehistoric times. T. sinensis is being used
Savitribai Phule Pune, almost in the same way in place of drug derived from T. cordifolia (Srinivasan et al. 2008) [9].
University, Ganeshkhind Road,
Pune, Maharashtra, India
The plant is designated in Ayurvedic Rasayanas to promote longevity, anti-stress, as an
~ 2313 ~

95) Dapoli respectively during April-May 2015. upper end of each cutting were sealed by market demand the said drug rank 29 th in their volume single layer of wax to reduce water evaporation. Nevertheless. On the basis of Before plantation.20 cm.84 cm. electrical conductivity.25±3. viz control (T0). Further.45±0. (T1) exhibited highest rooting percentage (67. The et al. Bavistin solution (Carbendazim Powder). and T5 was found significant at 95% ~ 2314 ~ . 400 ppm (T4) and 500 ppm (T5). 2012) [16. forest areas are the to maintain the plants exclusively organically. It is cleared that yield of biomass was determined by method described by the demand of ‘Guduchi’ drug obviously cannot be complete Gupta et al. Pune campus and Collage of The stem cuttings of T. the numbers of sprouted (BSI). the National Medicinal of size 5 x 20 feet containing nursery polythene bags having Plant Board (NMPB) recently launched a concerted effort to size 6 x 9” filled with soil medium. Maharashtra (Latitude treatment T1 but significant differences in mean value of all 18°33'22. cordifolia stem cuttings 45 DAP The maximum rooting was observed in T.05). cordifolia and T. In this context. ii) Effect of IBA on T. it may be an hour as described below.75±3. The cuttings were specifically recorded in treatment T1 (104. The maximum and minimum shoot lengths were apex and slanting cut at base.) and T0 prepared by considering position and direction of cup like (48./2015/AR significant differences in mean shoot length were not reported. leaf width as well as leaf length was slightly higher in Savitribai Phule Pune University. Shoot length mean value of nodes (Photo plate I)./2015/AR01 and No. a broad spectrum In India there are about 10.75. However. The cuttings thus prepared were treated with in 1% (w/v) 2010. The Tinospora drug has a wide therapeutic activity therefore it has ever increasing d) Plantation of cuttings and data collection demand in local as well as international market. The means differing significantly standardise the techniques for mass multiplication. Further distal part of cuttings (2-3 cm) companies exit of which about 750 are functional in was deep into different concentration of IBA solutions for half Maharashtra (Wankhade et al. cultivated source of plant origin. shoot length. Total eighty cuttings were 5000 MT with annual growth registered at 9. 100 ppm (T1).25%) was reported in 30 DAP to 45 having 2-3 nodes were prepared by taking horizontal cut at DAP.000 registered medical ppm (T2). The diameter of newly sprouted shoot was also higher in The field experiments were conducted in medicinal plant treatment T1 (2. 2013) [12]. 300 ppm (T3). for mass multiplication (Handique. 83. Considering Vegetative propagation assessments were tested in sunken bed the importance of Tinospora in India. Dr. Jain et al. from wild sources any more and more focused efforts pertaining to farming are crucial.1% (NMPB. 2016) [13] which propagation. To fulfil the supply demand e) Statistical analysis of data gap it is essential to develop propagation and agro technique The experiment was carried out following RBD (Randomized for the Tinospora species. petiole garden that has been established in the university campus of length. 2013) [8-10-13]. The major source of raw drug for collectors. weed control. there is complete lack of Block Design) with four replicates (n =4). The treated cuttings of both address these concerns and prioritized this important species the species were planted at 3-4 cm depth in nursery bags. The number of leaves. 02 for T. Veeraiah and Reddy. Standard organic practices [15] . Twenty cuttings were used for formulations. The total numbers of days taken for initial sprouting natural regeneration.159±15. (1998) [18]. Data were analyzed scientific approach on propagation and cultivation of by Analysis of variance (ANOVA) to detect significant Tinospora drug. shoots was higher in treatment T4 (1. 2014) [14]. Presently. 2008. in practitioners under Ayurvedic system while about 700. 17]. Pune (MH). Hence. Choudhary et al. present study was carried out to difference between means. inadequate species.92±5. disease and pest control was inevitable to promote commercial level cultivation of this implemented during the entire study period.) respectively. root length. cordifolia treated with 100 ppm IBA Forestry.5"E). leaf length and leaf width were recorded.58 cm. unrestricted anthropogenic exploitation. T3. Longitude 73°49'17. The healthy. The demand of T.50±5. depletion of wild stock of this valuable medicinal plant (Bapat petiole length.e. thumb size of about 15-20 cm long cuttings Enhanced rooting (16.54±0. cordifolia stem b) Preparation of stem cuttings cuttings treated with 100 ppm IBA (T1) i. Pune and received reference numbers shoot length was attained by treatment T1 (28. In recent days the large observations recorded after 30 DAP and 45 DAP for both the scale.0. 2014) [14].14 mm.75). different methods are considered as such as watering. The voucher specimen deposited to BSI.15±7. Single cutting utilization for the preparation of different ayurvedic was put into each nursery bag. improve or building up immunity system and body c) Treatments to stem cuttings resistance against diverse types of infections (Upadhyay et al. The taxonomic followed by treatment T2 (46.000 Ayurvedic drug manufacturing fungicide. was compared using Duncan’s (1955) multiple range test (DMRT) at the 5% probability level using the software SPSS Material and methods 16. for experiment was analysed for common agronomical parameters namely soil pH. a) Collection of plant materials and details of experimental site Results The samples of T. Variability in data was express as mean± standard error. sinensis (especially stem) i) Effect of IBA on T. for 10 min.) but BSI/WRC/Cert. 2012) evaluated under each treatment. The morphological data industry tangled with constricted cultivation and inadequate such as number of shoots. treatment T2.BSI/WRC/Cert.50. root-shoot ratio. number of leaves. increasing demand by the pharmaceutical and rooting of cuttings were recorded. Balasaheb Sawant Konkan Krishi Vidyapeeth. The care was taken species (Handique. The maximum Western Circle. sinensis respectively. diameter of stem. The garden soil used these parameters were not recorded (Table 1). First sprouting was authentication was carried out from Botanical Survey of India recorded 11 DAP. efforts for its replacement resulted into the in discriminate number of roots. Cuttings without any treatments considered as utilizes several plants as raw material obtained from wild and control.Journal of Pharmacognosy and Phytochemistry adaptogen.).5"N. Pune. cordifolia and T. 200 noted that there are currently about 2. cordifolia ranged from 2000 to each experimental treatment. 2010.000 of order to find out rapid and efficient pretreatment for the modern medicine system (Ganesan et al. cordifolia stem cuttings 30 DAP were collected from SPPU.

44a 6.77±4.) and dry root biomass (0.5±1.97±0.50±0.06b 2.08ab 1.14ab Shoot length (cm.25±0.77a 40.39) followed by treatment T1 (56. dry root biomass and total dry biomass was The maximum rooting percentage was recorded in control T0 also higher in treatment T1 (Table 2).12ab 1.16±0.36a 10.19±0.44b 35.05b 1. T0 (47.26).02b 0.72±1.95±0.25±0.63±14.26a 10.60a 23.34b 14.72±0.847±0.) 4.30a 11.16ab 2.29±0.74a Leaf width (cm.16±15.68a 5.09 mm.90±0.50±3.) 1.00).03b 0. of shoots 1.75b 42.02a 0.69±0.09a 14.49±1.21d 14.02b 0.50±3.17b 2.46 a 3.17±0.24a 20.33a 5.18a No.21b 2.03±0.97±1.48a 4.59a 6.03c 1.35±0.19±0.07±0.14±0.89a 12.25%) was observed in 30 DAP to 45 5.71±1. Table 1: Effect of IBA pre-treatment on T.41a 4.75 ± 2.66±0.45±0.) 3.50b No.1 cm/day respectively in T.25±8. cordifolia stem cuttings 45 DAP Parameters Pre-treatments T0 T1 T2 T3 T4 T5 Rooting percentage 47.08ab No.00±5. The maximum rooting was recorded in control T0 (12.91a 103.24d 15.00b 0.60ab 11.04a 6.68±0.25±0. The maximum numbers of roots was recorded in control T0 (5.22ab 2.45ab No.75±1. Nevertheless.) 0.50±0.09ab Shoot length (cm.40a 35. sinensis stem cuttings 30 DAP Parameters Pre-treatments T0 T1 T2 T3 T4 T5 Rooting percentage 47. Table 3: Effect of IBA pre-treatment on T.12b 1.12ab 2.) 7.54b 11.53a Leaf length (cm.12ab 3.89±0.46 mm) The effects of IBA on growth parameters of T.02b 0.09b 1.04± 0. of leaves 3.74±1.90±0.90a 24.45±0.03±0.07a 1.46±1.11±0.19a 12.33±0.11a 12.29a Diameter of stem (mm.) 0. sinensis respectively. (1. The diameter of newly sprouted shoot was (Table 3).).50±1.25±0.41ab 90.79±6.) were found higher in treatment T1 but significant differences in mean value significant for increasing diameter of newly sprouted shoot were not recorded.09±0.35±0.04a 6. The numbers of sprouted shoots was higher in control T0 present investigation.27±2.20a 20.12±10.98±0. The maximum numbers of newly sprouted shoots was in control T0 (Table 4).36a 10.60±5.09a 2. of roots 3.) 24.72±0.3±0.75±3.8±0.42±1.58b 104.05±0.23c 12.11 mm).13±0.36b No.06±0.74a 23.22±0.03c 1.52±0.08a 28.00 g.03d 2.50±7.) 6.02±0.15a 3.49±0.75±1.00±0.31±0.23b 83.09ab 1.17±1.33a No.48a 3. The fresh root biomass (0.00b 0.77a Petiole length (cm.17).130a 10.16±0.27±0.44).14a Leaf width (cm.30 g.38±0.95a 46.75±0.) 3. sinensis stem cuttings (14.e.) 5.3±0.01b 0.11).03±0.) 7.25) and root length (15. The average minimum (63.36a 5.10±0.35±11.12a 8.03b 2.25±3.83a 4.23a 79.74±0.40±0.31±7.28a 12.57±0.15±7.06±0.46±0.12b Dry shoot biomass (gm. treatment (4.35a 12. The maximum numbers of leaves were recorded propagated through stem cuttings did not reveal any significant in treatment T3 (10.30a 11. 30 DAP followed by T1 (3.25±0.) (Newly sprouted shoot) 2.57±0.5±0.14±0.19a 12.14±0.30a 10.23a 11. The means followed by the same letters within rows are not significantly different at the 5% level (DMRT).17±0.35a 4.48a).52a 26.38a 3.18±0.10±0.57±0.00±7.07a 1.86±0.73±0.00 a 0.04a 1.36a Fresh shoot biomass (gm.45a 4.72±0.11a 1.) (Newly sprouted shoot) 3.05 mm.) and T5 (3.48a Leaf width (cm.29a 4.00±4.57±0.08b 52.) 12.07a 16.17a 3.1±1.91±0.06a 6.69a Values mentioned in this table are mean ± standard error (SE) with four replications.33 cm/day to Increased rooting (16.35±1.10±0.82±0.33±1.50±2.) (Sprouted shoot) 2.02b 2.68a 15. 45 DAP dry shoot biomass.13a 4.18±0.42±1.93±0.05±0.30 g.36±0.72±0.08 a 5.00c Total dry biomass (gm. cordifolia stem cuttings 30 DAP Parameters Pre-treatments T0 T1 T2 T3 T4 T5 Rooting percentage 37. The means followed by the same letters within rows are not significantly different at the 5% level (DMRT).) 6.17a 0.29a 37.00 control i.54±0.92±0.61±0.67±0.90±0.43±0.58a Leaf length (cm.20±0.24a 3. while leaf width was higher in difference 30 DAPS.23b 46.50±5.15b 55.15±0.66±0. sinensis stem cuttings sprouted shoot was reported in control T0 (3.16a 11.02±0.25±3.92±0.3±0.77a 5.42a 4.15±0. First sprouting was observed 10 g.54a 4.14a 2.97±0. of leaves 8.57±0.92±0.24a 3.09±0.05a 1.06±0.71±0.48a 4.22a 23.) 7.58±0.99±0. Maximum fresh shoot biomass recorded in T1 (14.50±6.26 a 10.85±1.5±1.78±0. The maximum diameter of newly iii) Effect of IBA pre-treatment on T.43b 11.71a 17.22a 4.09±0.71±0.79a 5.40±0.44a 5.25a 3.15a 5.20a 14.41±0.54±0.50±3.85±3.) 3.56a 10.Journal of Pharmacognosy and Phytochemistry confidence intervals.50a Table 2: Effect of IBA pre-treatment on T.02±1. cordifolia cuttings during the DAP.84±0.28a Leaf length (cm.04b Diameter of stem (mm. total fresh biomass.70±7.09 cm) was slightly T0 (3. of shoots 1.30±0.41a Root length (cm.) and T1 (3.41a 5.19a 10.06±0.75a No.50a 3.04ab 2.08ab 1. and maximum growth speed was reported to be 2.00±0.01c Total fresh biomass (gm.30±0.42±0.11a 2.98a 4.04±0.73a 5.11).61a Diameter of stem (mm.05b 1.35±1.75 ±3.92±5.03b Values mentioned in this table are mean ± standard error (SE) with four replications.00±0.21±4.55±1.) 13.32±0.5±0.02b 0.23b 67. of shoots 1.71±0.24±0.47±7.75±0.16a 5.54b 42.45±0.30±0. The fresh root biomass.02a 0.) were found significant DAP.64±0.) 4.54±0.54±0.40a 13.07ab 2.00±0.24±0.45a 8.16 mm.25±0.83 ±6.76b 11.35±0.00±4.84±0.40±0.25±0.).05d 2.16±0.25±0.6±0.01a 0.11b Fresh root biomass (gm.73±0.8±0.05a 1.00b 0. The maximum numbers of roots observed in treatment T0 (1.96±5.44a 37.15a 6.35±1.79a 6.06ab 2.17±0.16ab 2.85±0.17a Petiole length (cm. ~ 2315 ~ .98a Root-shoot ratio 0.56a 38.96a 5.05a 3.84a 84.40±0.75a 55.30±1.23±0.39±0.85±0.72±0.08ab 1.05 mm.) 48.59±0.50±1.20±0.55±0.76a 5.00±3.02b Dry root biomass (gm.19±0.40±0.50a 3.05a 1.23c 11.05ab Shoot length (cm.53±0.33±0.75±0.12ab 1.75±0.09b 1.55±7.42±0.43a Petiole length (cm.14a 1.51a 3.01b 0.44±0.62a 5.45a 3.290±0.5±0.) followed by T3 iv) Effect of IBA pre-treatment on T. of leaves 3.45±0. also higher in treatment T1 (2.39a 5.03±0.34ab 14.) 0.

00a 8.4 ± 0.05±0.33bc 10.16 3.27a 4.13ab 3.69ab Petiole length (cm.65±0. The means followed by the same letters within rows are not significantly different at the 5% level (DMRT).66±0.68 ± 0.82a 18.75±4.73a 18.03±2.17a 11.01a 15.30±3.37 97.51b 9.67±0.) 91.61a 11.70a 10.81±0.81a Root-shoot ratio 0.53 3.9 3.01a Values mentioned in this table are mean ± standard error (SE) with four replications.) 11.08±0.05a 0.01 16.5± 4.54a 47.30±0.08a No.22±0.81±0.05a 1.) 0.25±8.17±3.05a 0. cordifolia 45 DAP ~ 2316 ~ .29 4.71±0.) 21.) 4.03±3.00a 0.24±0.73±0.12a 1.25a 17.11 ± 0.25a 16.19±0.29 ± 0.08± 0.33a 50 4.10a Fresh root biomass (gm.50±1.75±2.29±0.Journal of Pharmacognosy and Phytochemistry Table 4: Effect of IBA pre-treatment on T.50±0.) 20. Photo plate I Nursery bags in sunken bed Plantation of cuttings New sprouted shoot Cuttings with cup shape node T.00a 0.16± 0.00e a a a a Total dry biomass (gm.30±0.01a Dry root biomass (gm.) 0.88a 10.58a 11.50±0.59c 10.15 0.82 19.34a 18.74±2.27a 53.70a a b b No.29a 3.20± 10.17 3.68 83.91±0.23±0.02a 0.65 ± 0.42± 14.51 ± 0.00d 0.24 ± 0.15a 14.) 16.35a 11.45a 16.91± 0.38±0.27 22.00a 0.08a 1.20±0.48 4.25±0.75± 1.43±0.) 4.42±0. sinensis 30 DAP T.01a 0.45±7.00b 0.25 4.63±0.45 ± 1.63±0.60±1. of shoots 1.43bc 10.42±3.83±0.33a 12.10± 0.43a a 94.36a Leaf width (cm.2a 22.10 3.71a 22.08a Dry shoot biomass (gm.65±0.27 4.34b 10.16±0.40±0.55 4.00c 0.61±0.07± 1.45± 0.36a 11.36±0.) 12.46a No.77±1.5±0.09±0.14± 0.5 ± 0.75± 0.03a a a ab ab Diameter of stem (mm.10±1.37±0.45a 11.75±5.25c 4.53a 3.98 98.00c 0.25±0.00d 0.25b 4.18±0.73±0.19±0.22 ± 1.33a 3.85±0.01b 0.34a 11.12a 1.75±7.39a 56.26a 53.72 22.15± 0.) (Sprouted shoot) 3.56a 4.28a Leaf length (cm.19 ± 0.14a a a a Shoot length (cm.25±1.40 ± 0.86±0.00c 0.42bc Fresh shoot biomass (gm.00b 9.21± 0.60± 0.12ab 10.02a 0.56± 1.80±0.5±1.48± 4.62±0.33a 3.13 ± 4. of roots 5.08a 12.50a 10.30 85. of leaves 8.01e a a a a Total fresh biomass (gm.50± 0.93±0.23±0.) 11.29b Root length (cm. sinensis stem cuttings 45 DAP Characters Pre-treatments T0 T1 T2 T3 T4 T5 Rooting percentage 63.11a 1.00ab 7.27±0.05 3.18±0.18 ± 0.96±0.09±1.16±0.

one need to also carried out study on macro-propagation of T. cordifolia treated with 100 ppm IBA (T 1) decrease the rooting percentage. the stem (84. Rao et al. This finding was endorsed exhibited significant rooting percentage. The exogenous application of auxin cuttings of rose with 600 mg/liter of IBA. excessive concentration of IBA tends to cuttings of T. sinensis displayed poor performance to IBA T. Manzanilla) [30] and confirmed that. in T. On the treatments after three month of plantation. treatment which may be due to it’s an inhibitory effect. cordifolia. The results of present investigation pertaining T. 2007. (2010) [25]. the stem cuttings treated with different The stem cuttings of T. Maximum plant length (364.73 to use of excess water resulting into decaying of stem cuttings. rooting and root length when compared to found that. as well as dry biomass however. sinensis which was found slightly higher than T. (2003) also reported total sprouting process were completed within 45 DAP. cordifolia best rooting performance (96%) safe and inexpensive route of multiplication. IBA inhibit shoot growth in rooted elongation in plant tissue. rooting percentage. cordifolia showed better rooting response (86%) with 200 and 2. Kim and Mulkey (1997) [29] also found similar synthetic analog like IBA were more effective than IAA. numbers of roots. Thus propagation through cuttings was faster. Conclusion In T. sinensis Discussion treatment could have an inhibitory effect on initiation of It has been evident that the auxin has been involved in the rooting though it acts as a stimulant of rooting. However. IBA pre-treatment to the cutting of T. cordifolia provided below. cm) and number of branches (3. Sun and Bassuk different physiological processes such as differentiation. fresh and propagation can be taken up which indeed required low capital dry root biomass. The quality planting materials (QPMs) of these poor performance. some observations were taken along with cordifolia but shoot elongation was enhanced greatly after growth performance to get quality seedlings which reduce the sprouting. leaf width. Thus the present study showed deviation from above findings. The to 5. The IBA treatment has been a powerful tool in many forestry plant species to also stimulated ethylene synthesis. without application of growth regulator hormone. Heavy damage by Spodoptera and thrips to tender leaves and 300 ppm concentrations. Auxin induced promotion stimulate adventitious rooting (Hartmann et al. use well drained soil and organic farming conditions for The study revealed that. but application of cuttings. 2013) [21-25-23]. sinensis. total fresh thereby recording better performance in untreated or control. sinensis. cuttings The average minimum and maximum growth was 3.5%). it was observed that.93 cm/day reported similar performance without application of IBA. Thus it might be concluded that IBA species can be easily produced throughout the year for large ~ 2317 ~ . revealed that T. cordifolia and T. 2007. although sprouting time was considerably higher in T. The additional recorded cordifolia corroborated well with the results reported by observations and results along with few suggestions are Mishra et al. The of ethylene production associated with increased ACC presence of natural auxin in plants (IAA) is responsible for synthase level which was inversely correlated the bud break of induction of rooting in several plant species. The overall results showed that. Ahmed et al. stem were observed in seedlings those have been propagated (2007) [27] through seeds. Mature vine cuttings of T. In nursery practices. There after inhibitory effect of IBA on rooting of hardwood cuttings of no rooting was reported. risk of damage to the plants. cuttings of both Tinospora species were susceptible other auxin as well as control.The care was taken to prevent the water which were treated with 100 ppm of IBA significantly logging and compactness of soil in polythene bags. It was increased sprouting. exhibited significant sprouting. treated with 3000 ppm IBA displayed maximum rooting In the present investigation. (2000) basis of this observation it was recommended that. cuttings Eugene et al.42) were recorded in same IBA Therefore seedlings were watered at interval 4-5 days. The treated cuttings of T. great influence on rooting and growth performance in different Khajehpour et al. cordifolia treated with 100 ppm IBA concentration of IBA failed to display significant results. sinensis 45 DAP Effect of IBA on rooting of T. europaea var. Blythe et al. However.Journal of Pharmacognosy and Phytochemistry T. cordifolia. Cuttings species of Tinospora are currently facing dearth of biomass without hormone treatment found to enhance diameter of required for commercial utilisation and therefore large scale newly sprouted shoot. Ling et al. sinensis. (2014) [30] investigated effect of different kinds of plants was found to be due to its greater stability within concentrations of IBA on stem cuttings of olive (Olea tissue and during storage (Bassuk 1992. sinensis displayed investment. Both The initiation of first sprout was observed on 10 DAP. study by Warrier et al. cell (1991) [28] found that. Its phenomena in intact primary roots of maize (Zea mays). The all rooting and with present study. propagation or cultivation of T. Peach rootstocks treated with 4000 ppm IBA. 1997) [20].17 cm/day respectively during the time of investigation in cuttings of T.

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