Métodos de obtenciòn de Km y vmax

140.0

120.0

100.0

80.0 Va lor obs erva do

v (uM min-1)

Ajuste no lineal (s olver)

Lineweaver-Burk
Ea die-Hofstee
60.0 Hanes -Wool f

40.0

20.0

0.0
0 20 40 60 80 100 120

[s] (uM)
 Ajuste no lineal (solver) Lineweaver-Burk
Valor observado valor calculado
[S] (uM) v (uM min-1) v (uM min-1) 1/[s]
2.5 28.0 28.3 0.081 0.4000
4 40.0 40.4 0.136 0.2500
10 70.0 70.5 0.226 0.1000
20 95.0 93.8 1.458 0.0500
40 112.0 112.4 0.147 0.0250
100 128.0 127.6 0.199 0.0100
2000 139.0 139.5 0.228 0.0005000
10000 140.0 140.0 0.001 0.0001000
2.477

m=
b=
r2 =

km = 9.889 uM km =
vmax = 140.2 uM min -1
vmax =
r= 0.9999 r=
Lineweaver-Burk Eadie-Hofstee Hanes-Woolf
valor calculado valor calculado valor calculado
1/v v (uM min-1) v/[s] v (uM min-1) [s]/v
0.03571 28.0 11.2 28.1 0.08929
0.02500 40.0 10 40.1 0.1000
0.01429 70.1 7 70.2 0.1429
0.01053 93.6 4.75 93.6 0.2105
0.008929 112.4 2.8 112.3 0.3571
0.007813 127.9 1.28 127.7 0.7813
0.007194 140.0 0.0695 139.7 14.39
0.007143 140.6 0.014 140.3 71.43

0.07152 m= -10.01239 m=
0.007105 b= 140.449354 b=
0.99996 r2 = 0.99957287 r2 =

10.07 uM km = 10.01 uM km =
140.7 uM min -1
vmax = 140.4 uM min -1
vmax =
0.9999 r= 0.9999 r=
 Hanes-Woolf
valor calculado
v (uM min-1)
26.73
38.37
68.00
91.57
110.8
126.7
139.4
140.0

0.007136
0.07569
1.000

10.61 uM
140.1 uM min-1
0.9997