Está en la página 1de 11

GUIDELINES ON LIQUID CHEMICAL WASTE DISPOSAL

(FOR WASTES FROM CRUDE PROTEIN ANALYSIS)

DATA REFERENCE LINK
1 Kjeldahl analysis disposal - SINK* (p. 3) Information on How to Dispose of http://osuseafoodlab.oregonstat
*Check pH of your waste, it should be between pH 4-10. (p. 1) Chemical Waste Generated in the e.edu/sites/default/files/secure_
Laboratory docs/chemical_waste_disposal.p
df
Author:
Oregon State University, College of
Agricultural Sciences, Seafood
Research and Education Center
2 13. Using an appropriate device for handling very hot glassware, remove the digestion tube from the holder. Kjeldahl Standard Operating https://www.foxscientific.com/kj
The dark solution may be disposed of by pouring it down a drain with copious amounts of water. **Note 2 Procedure eldahlsop.html
at the end of the Distillation section.
Author:
** Note 2. The drain disposal assumes use of a Cu, Ti or similar catalyst. Use of a mercury (Hg) catalyst Fox Scientific, Inc.
requires proper disposal per local environmental regulations. Mercury catalyst should NOT be poured down
a drain that empties into a public waste system.
3 Several catalysts are available for digestion, including mercury, copper, and copper/titanium. Choice of 3.2 Nitrogen Determination by http://www.foragetesting.org/la
catalyst will depend on the difficulty of breakdown of the peptides in the sample protein to be analyzed and Kjeldahl (Block Digestion) b_procedure/sectionB/3/part3.2.
environmental problems associated with the disposal of the waste containing the catalyst. htm
Author:
National Forage Testing Association
[NFTA] (www.foragetesting.org)
4 A chemical waste that is not listed by the EPA is still a hazardous waste if it has one or more of EPA's four Waste Disposal Guide http://www.ehs.msu.edu/waste/
hazardous characteristics: ignitablity, corrosivity, reactivity or toxicity. (p. 5) programs_guidelines/WasteGuid
Author: e/wastedisposalguide.pdf
5 Accumulation Time
Michigan State University
• Chemical wastes shall not be accumulated for longer than 90 days. Each container shall be labeled with a
collection start date and chemical constituents when waste is first added to the container. (p. 6)
6 “Corrosive Waste”

• Nitric acid exceeding 40 percent concentration • Perchloric acid • Hydrogen peroxide exceeding 52 percent strength by weight • Nitrihydrochloric or Nitrohydrochloric acid diluted (p 10) 11 Contaminated Debris From Laboratories This includes gloves. The laboratory is responsible for separating hazardous and nonhazardous waste and preventing accidental exposure of custodians to hazardous materials. and Aqueous Solutions Do NOT mix strong inorganic acids or oxidizers with organic compounds. fill containers to about 90% of container volume. paper. it is a nonhazardous waste and may be disposed of in the dumpster. any waste contaminated with trace levels of a poison or carcinogen should be collected for incineration. plastic. For example. 13) 12 Non-contaminated Debris from Laboratories Work practices must be followed by all University Department laboratory staff in disposing and separating nonhazardous waste from hazardous waste. (p. (p. 7) 7 Do NOT put corrosive or reactive chemicals in metal cans. Avoid mixing concentrated acids and bases together in the same container. 9) 8 For liquids. If the debris contains any of the constituents in concentrations greater than those listed in Appendix H (heavy metals. and other inert debris contaminated with hazardous chemicals. Keep acids. If it comes from the cleanup of a hazardous material spill it is a hazardous waste. (p. sharps or broken glass into the normal paper waste receptacles. 31 of document) . These liquids must be packaged in their own separate shipping container. 9) 9 Acids. ethidium bromide (mutagen) or phenol (poison) contaminated solid debris is best disposed of by incineration. 13) 13 Chemical Compatibility Table (found on page 18. Do NOT place hazardous waste. (p. Do NOT fill containers to the top. If it is neither of these. 10) 10 Corrosive Materials The following corrosive liquids shall not be mixed with any other hazardous waste under any circumstances. (p. and Appendix C p. Leave at least 2 inches of space in 5-gallon liquid waste containers to allow for liquid expansion and pumping. Bases. bases or aqueous solutions containing heavy metals (Appendix H) separate from other wastes. In some cases it is not prudent to dispose of nonhazardous waste into the dumpster.5 is a corrosive waste. • An aqueous solution which has a pH less than or equal to 2 or greater than or equal to 12. the contaminants and the concentration of contaminants. (p. organics and pesticides) or Appendix I (severely toxic compounds) it is a hazardous waste. In general. Whether this material is a hazardous waste depends on how it is generated.

Contaminated pasteur pipettes are considered sharps and need to be disposed of in a sharps container.txt?ZyActionD=Z yDocument&Client=EPA&Index= 1995%20Thru%201999&Docs=& Author: Query=&Time=&EndTime=&Sear Office of Small Business chMethod=1&TocRestrict=n&Toc Ombudsman. U. These contaminated items shall be placed in biohazard bags and decontaminated by autoclaving. bio.S. Environmental =&TocEntry=&QField=&QFieldYe Protection Agency ar=&QFieldMonth=&QFieldDay= . Double or triple bagging may be required to avoid rupture or puncture of the bags. (p.cromlab.pdf disposed or handled as hazardous waste.gov/sites/prod disposal problems associated with mercury.13) Author: http://www.epa.epa.14 Contaminated Solid Waste Contaminated solid waste includes cloth. 1) Nitrogen in Water and Biosolids by uction/files/2015- Automated Colorimetry with 10/documents/method_1687_dr 16 15. (p 26) 15 2.S.exe/9101CHKQ. 14) (p.pdf Method 1688: Total Kjeldahl Nitrogen in Water and Biosolids by Automated Colorimetry with Preliminary Semi- automatic Digestion Author: U. plastic and paper items that have been exposed to agents that are infectious or hazardous to humans.2 Samples containing strong acids or bases are hazardous and must be either neutralized before being Preliminary Distillation/Digestion aft_2001.gov/Exe/ZyNET for Small Laboratories (1998) . Environmental Protection /Metodos/EPA/1600/1688- Agency.es/Articulos U. or plants.3 Cupric sulfate has been substituted for the historically-used mercuric sulfate due to toxicity and waste Method 1687: Total Kjeldahl https://www. (p. animals.S. Environmental Protection Agency 17 Environmental Management Guide https://nepis.

Environmental Protection hod=1&TocRestrict=n&Toc=&Toc Agency Entry=&QField=&QFieldYear=&Q FieldMonth=&QFieldDay=&IntQF ieldOp=0&ExtQFieldOp=0&XmlQ uery=&File=D%3A%5Czyfiles%5CI ndex%20Data%5C00thru05%5CT xt%5C00000000%5C100049DH.gov/Exe/ZyNET for Small Laboratories (2000) .TXT?ZyActionD=Z EPA 233-B-00-001 yDocument&Client=EPA&Index= 2000+Thru+2005&Docs=&Query Author: =&Time=&EndTime=&SearchMet U.epa. 24) .tx (p.txt&User =ANONYMOUS&Password=anon ymous&SortMethod=h%7C- &MaximumDocuments=1&Fuzzy (p 11-12) Degree=0&ImageQuality=r75g8/r 18 75g8/x150y150g16a/i4a25&Displ ay=hpfr&DefSeekPage=x&Search (p 17) Back=ZyActionL&Back=ZyActionS 19 &BackDesc=Results%20page&Ma ximumPages=1&ZyEntry=1 (p 22) 20 Environmental Management Guide https://nepis.S. &UseQField=&IntQFieldOp=0&Ex tQFieldOp=0&XmlQuery=&File=D %3A%5CZYFILES%5CINDEX%20D ATA%5C95THRU99%5CTXT%5C0 0000033%5C9101CHKQ.exe/100049DH.

com/ca Methods and Apparatus talogs/labconco/pdf/KJELDAHLgu ide.expotechusa.43) 75g8/x150y150g16/i425&Display 21 =hpfr&DefSeekPage=x&SearchBa ck=ZyActionL&Back=ZyActionS&B ackDesc=Results%20page&Maxi mumPages=1&ZyEntry=1&SeekP age=x&ZyPURL (p. t&User=ANONYMOUS&Password =anonymous&SortMethod=h%7C - &MaximumDocuments=1&Fuzzy Degree=0&ImageQuality=r75g8/r (p.PDF Author: Expotech USA . 111) 22 Kjeldahl Nitrogen Determination http://www.

ncbi.23 Therefore. (Chapter 8: Management of Waste) Author: National Center for Biotechnology Information [NCBI] . composition of: Distillate : Acid digestion mixture after distillation: H3BO3 (aq) and NH4Cl (aq) Na2SO4 (aq) and H2O 24 Prudent Practices in the Laboratory: https://www.gov/b Handling and Management of ooks/NBK55885/ Chemical Hazards: Updated Version.nih.nlm.

csbsju.edu/environm In-Lab Disposal Options.gsmc.titech. ental-health/environmental- management/waste- .edu/environ Laboratory Waste Management mental-health- Author: safety/programs/hazardous- College of ST. pH = 7 and above Japan (p. 7) 27 http://www.25 26 2016 Guide to Appropriate http://www.jp/hai Laboratory Waste Control kibutu/H28tebiki%20eng. Benedict – Saint John’s waste-management/laboratory- University waste-management Environmental Health and Safety 28 29 https://protect.ac.pdf pH= n/a Author: General Safety Management Center.iu.

html Author: https://protect.30 Attachment A: Disposal Options for management/waste-guide/in- Specific Chemicals lab-disposal.maine.iu.umeche.html#A1 Author: The University of Maine Department of Chemistry .edu/doc/envir Indiana University onmental- Environmental Health and Safety health/waste/Waste%20Program %20Attachment%20A.pdf 31 http://chemistry. Disposing of Chemicals edu/Safety/Disposal.

32 33 Disposal of Laboratory Wastes: https://cms.pdf Environmental Health and Safety Office Ball State University (p.bsu. 2-3) 34 (p 14) .edu/- Requirements for Chemical Disposal /media/www/departmentalcont to Sinks and Drains ent/facilities/pdf/bsu%20sewer% 20disposal%20nov%2016%20201 Author: 1.

35 (p14-15) 36 (p. 15) .

the composition of the distillate and the acid digestion mixture after distillation are as follows: Distillate : H3BO3 (aq) and NH4Cl (aq) Acid digestion mixture after distillation: Na2SO4 (aq) and H2O The ammonium chloride and sodium sulfates are both soluble salts. the use of NaOH solution to neutralize diluted acidic liquid wastes.google.146786187.37 Inorganic Acids (on p 16) Inorganic Bases (on page 17 of document) 38 Standard Operating Procedures For https://www.wsu.11).  Strongly acidic or strongly basic liquid chemical wastes should be diluted first before neutralization.g.d.docx&usg=AFQjCNFxTos47CB (p 2) y14_1BpNSRIKOv5efQA&bvm=bv . . and thus.ph/url? Hazardous And Particularly sa=t&rct=j&q=&esrc=s&source= Hazardous Chemicals for Boric Acid web&cd=2&cad=rja&uact=8&ved =0ahUKEwjcuPz8ho_SAhUMpJQK Author: HU4ODJ8QFgglMAE&url=https% Environmental Health and Safety 3A%2F%2Fehs.  Acidic or basic liquid chemical wastes should be neutralized to a pH of ≥7 before drain disposal. they can be disposed via drain disposal.  Neutralization of acidic or basic chemical wastes can be done using common acid-base neutralization procedures (e.  In crude protein analysis via Kjeldahl method (AOAC Method 2001. The boric acid used in the method is only of 4% concentration.com.5 are considered highly corrosive wastes and are not suitable for drain disposal.edu%2Flabsaf Washington State University ety%2Fsoptemplatedocuments% 2FBoric%2520Acid_2015-11- 25. the use of HCl solution to neutralize diluted basic liquid wastes). can be disposed via drain disposal.dGo GENERALIZATIONS:  Liquid chemical wastes with pH of ≤2 or ≥12.