Documentos de Académico
Documentos de Profesional
Documentos de Cultura
MTh 7-10AM
Sample View
Fluorescence Microscope
Sample View
Phase Contrast Microscope
Sample View
Differential Interference Microscope
Sample View
Scanning Electron Microscope
Sample View
Transmission Electron Microscope
Sample View
Mechanical Parts
- Base -Nosepiece
- Pillar -Dust shield
- Inclination Joint -Coarse Adjustment Knob
- Arm -Fine Adjustment Knob
- Stage -Condenser Adjustment knob
- Draw tube -Iris Diaphragm Lever
Optical Parts
Terms to Know
Magnifying Power Numerical Aperture
10x, 40x, 100x
0.30, 0.65, 1.30
-ability to magnify the
- Light refracting capability
view of an object
Resolving Power
-ability to reveal adjacent details as separate and distinct.
-typical compound microscope has a resolving power of 0.2-0.4 nm
Working Distance
- distance between the lens of the objective and the object on
the slide when it is in focus.
Differences between the Old and New Models
Old New
Objectives (3) LPO, HPO, OIO (4) Scanning, LPO,
HPO and OIO
Not spring-loaded Spring-loaded
Adjustment Separate Joined
Knobs
Illumination Ambient Light Light Bulb
(Light Source) using Mirror
Stage Clips Immovable Movable
Differences between Compound Microscope and
Stereoscope/Scanning Microscope
Compound Stereoscope
Magnification 10x-100x 4x
Example:
Magnifying Power of HPO = 40x
Magnifying power of Ocular =10x
40 x 10= 400x
When using the Ocular micrometer:
Stage
Number of Lines in ocular = 9
Example:
Number of spaces in ocular micrometer = 12
Computed Calibration Factor = 0.0055mm
In nanometers:
0.0648 mm x 100 nm = 6.48 nm = Actual size of object (nm)
1mm
Computing for the Magnification of
the Illustration
Size of Drawing / Actual Size of Object
Example:
Size of Drawing = 3 cm or 30mm
3cm
Actual size of object = 0.0648 mm
30mm/0.0648mm = 462.96x
or 463x