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1.

The following data have been obtained for two different initial enzyme concentration for an enzyme
catalyzed reaction :

V (g/Lmen) {(Eo = 0,015 g/l)} S(g/L) V (g/Lmen) {(Eo = 0,00875 g/l)}


1.14 20.0 0.67
0.87 10.0 0.51
0.70 5.7 0.41
0.59 5.0 0.34
0.50 4.0 0.29
0.44 3.3
0.39 2.9
0.35 2.5
Find : Km, Vm for (Eo = 0,015 g/l) and (Eo = 0,00875 g/l)}, and k2

2. D Thornton and co-workers studied the hydrolysis of succrose at pH = 4.5 and 25 Cusing crude invertase
obtained from bakers yeast in free and immobilized for m . The following innitial velocity data were
obtained with 408 unit of crude enzyme ( 1unit= quantity of enzyme 1 Umol of succrose/mi9n when
incubated with 0.29 M succrose in a buffer at pH 4.5 and 25 oC

Vo (mmol hydrolyzed /L min)


Free Enzyme Immobilized Enzyme So (mol/L)
0.083 0.056 0.010
0.141 0.098 0.020
0.183 0.127 0.030
0.222 0.149 0.040
0.250 0.168 0.050
0.330 0.227 0.100
0.408 0.290 0.290
Find : Km, Vm for free and immobilized enzyme

3.The hydrolysis of urea by urease isan only partially understood reaction and shows inhibition. Data for the
hydrolysis of the reaction are given as follow:

Substrate Concentration 0.2 M 0.02 M


1/v I 1/v I
0.22 0 0.68 0
0.33 0.0012 1.02 0.0013
0.51 0.0027 1.50 0.0022
0.76 0.0044 1.83 0.0032
0.88 0.0061 2.04 0.0037
0.10 0.0080 2.72 0.0044
1.15 0.0093 3.64 0.0057
Where v = moles/Lmen and I is inhibitor molar concentration.

A . Determine the Km for this reaction

B. What type of inhibition reaction is this?

C.Based on the answer to part b, what is the value of Ki ?.


1. Kinetika Enzimatik Dengan Inhibitor noncompetitive

E S
k 1
ES
k2
EP
EI ESI ; Vo k 2 ES; Vmax k 2 ET
EI dan ES I

Km
E S ; K E I ; K ES I dan E E ES EI ESI

ES I
EI I ESI T

ET E ES
E I ES I atau K E K E E I K ES ES I
K I KI I T I I

2 A plant scale of Stirred Tank Bioreactor from pilot scale of 80 l to 10.000 l is being considered. Using
geometric similarity, determine the parameters : P/V and Q . The criteria chosen for scale up are: Constant
NDi
2

Power and NRe = Catatan : V Di3 , P N3 Di5, Q NDi3 , P/V N3Di2 , Q/V N

a. Design P/V

Kriteria scale-up Kapasitas 10.000 L

P = konstant

N Re = konstant
b. Design Q

Kriteria scale-up Kapasitas 10.000 L

P = konstant

N Re = konstant

3. Jelaskan cara memperoleh enzim gluco amylase dari Saccharomyces diataticus, mulai dari persiapan bhn
baku, inokulum, proses fermentasi, analisa hasi dan uji aktifitasnya