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Poster Session 10

Submission ID: 1345

MORINGA OLEIFERA AND IMPORTANCE IN PHYTOTHERAPY

1 2 2
FILIZ CEYLAN , AHSEN SEVDE INAR , ALEV NDER

ABSTRACT
The Moringa genus is known as a plant that can cope with all kinds of climate conditions and
is therefore widely grown throughout the world. The Moringa oleifera, a species unique to India, is
also grown in tropical and subtropical regions of the world. It is commonly known as a "drumstick
tree" or "horseradish tree." This species is a fast-growing tree native to South Asia. When examined
regarding both its content and its effects, M. oleifera has made a reputation as a species called "miracle
tree." Minerals, vitamins and other phytochemicals are also very rich in this species, and it is very
popular in Ayurvedic medicine. It can even provide all the needs of the human body. Other notable
effects include antioxidant, anticancer, anti-inflammatory, antidiabetic, antimicrobial,
hepatoprotective, antifungal, antiviral and milk boosting effects. Besides, it is mentioned
economically, also used in water cleaning treatment. Especially in under-developed countries, it has a
place as additional food. Moringa oleifera, which has such a significant effect, will be tried to be
introduced to this preliminary study because it is evaluated as phytotherapy with the aid of this poster
to be presented.

KEYWORDS
Moringa oleifera, phytotherapy, drumstick tree

Corresponding Author: FLZ CEYLAN, ceylanfiliz8@gmail.com


1
1 NOLU ALTNDA SALIKLI YAAM MERKEZ, ANKARA TRKYE
2
ANKARA NVERSTES, ECZACILIK FAKLTES

1391
Poster Session 10
Submission ID: 1346

STEVIA REBAUDIANA: A POTENTIAL BOON FOR HUMAN


HEALTH

1 1 1
KHAZINA AMIN , SENAY OZGEN , ZELIHA SELAMOGLU

ABSTRACT
A high potency low calorie bio-sweetener Stevia has been grown in many countries around
the globe for its sweetening and health promoting properties. Besides of making the world a sweeter
place, Stevia is well known for its efficient therapeutic and pharmacological agents which possess
antimicrobial, antifungal, anticarcinogenic and antioxidant activity. Leaves of Stevia have been used
from the ancient times to cure various chronic and non-chronic diseases such as diabetes, cancer,
cardiovascular disease, obesity, renal disease, inflammatory bowel disease and dental cavities. The
phytoconstituents of this plant, steviosides, rebaudiosides A-F, steviolbioside and dulcosides, were
found to be nontoxic in many research studies. Stevioside is the main herbal sweetener that is 100-350
times sweeter than the sucrose. It has pleasant taste, good solubility in water and safely metabolized by
the body without any toxic effect. High nutritional profile of Stevia leaves makes it superior to other
sugar substitutes. There is an increased demand in Stevia production from past decade due to fast
growing trend in diet foods or low calorie foods and beverages. Markets exist for Stevia but still there
is lack in its efficient production strategies. The great interest in Stevia has fueled many studies on it to
meet the rising food market demands. Research needs to be directed on Stevia and its metabolic
pathways to explore full potential regarding benefits on human health. This review article summarizes
the existing literature on Stevia cultivation, nutrition, therapeutic and pharmacological properties to
provide a baseline for new research on Stevia and its metabolites.

KEYWORDS
steviolbioside, steviosides, rebaudiosides

Corresponding Author: KHAZNA AMN, khazinaamin@gmail.com


1
MER HALSDEMR UNVERSTY

1392
Poster Session 10
Submission ID: 1347

ANTIMICROBIAL ACTIVITY OF SATUREJA HORTENSIS


ESSENTIAL OIL ON MICROORGANISMS ISOLATED FROM
DIABETIC FOOT ULCERS.

1 1 2 1 3
SABIHA AYDOGDU , ULKU ALTOPARLAK , SABAN KORDALI , ICLAL AGAN , DEMET CELEBI

ABSTRACT
BACKGROUND Satureja hortensis is a well-known aromatic and medicinal plant. It has also
been used as a folk remedy to treat various ailments such as indigestion, diarrhea and infectious
diseases. The highest proportion of the chemical components of the esential oil are carvacrol and
thymol. OBJECTVE This study aimed to evaluate the antimicrobial activity of carvacrol extract of
Stureja hortensis oil on microorganisms isolated from diabetic foot ulcers. MATERIALS and
METHODS Satureja hortensis oil was isolated by hydrodistilation method. The oil composition was
analyzed by Gas Chromatography - Mass Spectrometry (GC-MS), thus obtaining the crude essential
oil extract used for antimicrobial tests. Microorganisms were obtained clinical isolates from Atatrk
University Hospital. Microdilution and Disc Diffusion method were used to determine the
antimicrobial activity of the extract against 9 diabetic foot agents including Pseudomonas aeruginosa,
Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus epidermidis, Acinetobacter baumannii,
Staphylococcus aureus, Enterobacter aerugenes, Candida albicans and Escherichia.coli. RESULTS:
The minimal inhibition concentration (MIC) values (g/ml) of the carvacrol extract of essential oil
were determined for P. aeruginosa (33.75 g/ml), K. pneumoniae (16.08 g/ml), P. mirabilis (MIC
<1.05g/ml), S. epidermidis (MIC <1.05g/ml), A. baumannii (MIC <1.05g/ml), S. aureus (MIC<
1.05g/ml), E. aerugenes (8.4 g/ml), E. coli (8.4 g/ml) and Candida albicans (MIC < 1.05g/ml). In
the disc diffusion method; the inhibition zones were measured as 8 mm for P. aeruginosa, 16 mm for
K. pneumoniae, 15 mm for P. mirabilis, 30 mm for S. epidermidis, 30 mm for A. baumannii, 30 mm
for S. aureus, 21 mm for E. aerogenes, 30 mm for C. albicans and 14 mm for E.coli. CONCLUSION:
Carvacrol extract of Satureja hortensis showed antimibrobial effects on tested microorganisms. Plant
extracts should be considered when used in part of replacement treatment.

KEYWORDS
Satureja hortensis, Antimicrobial Activity, Diabetic foot ulcer

Corresponding Author: SABHA AYDOGDU, sabiha_sens@hotmail.com


1
ATATURK UNVERSTY, SCHOOL OF MEDCNE, DEPARTMENT OF MEDCAL MCROBOLOGY, ERZURUM
2
ATATURK UNVERSTY, FACULTY OF AGRCULTURE, DEPARTMENT OF PLANT PROTECTON, ERZURUM
3
ATATURK UNVERSTY, VETERNARY FACULTY, ERZURUM

1393
Poster Session 10
Submission ID: 1348

EFFECTS OF MEDICINAL AND AROMATIC PLANTS ON INSECTS

1 1
ZHAL SATI , TUBA AKDENIZ FIRAT

ABSTRACT
Our country has a rich flora and a large number of medical and aromatic plants. The number
of species is about 9,000. While almost 20.000 species used for medical purposes in the world, this
figure is about 500 in our country. In addition to the medical and pharmaceutical industry, these plants
are benefited from many other areas. One of these areas is agriculture. Today, depending on the
increase in the use of pesticides, environmentally friendly alternative methods which do not disturb
natural balances are used in agriculture. Medicinal and aromatic plants are also benefited for this
purpose. The important properties of plants on killing microorganisms and human health have been
investigated in laboratories since 1926. Studies have been conducted on the use of extracts and
essential oils against various insects and alternative methods of controlling weeds. However, due to
the fact that there is a large amount of plants and insects, these studies will continue. In this study, in
plant health subject, the positive and negative effects of medicinal and aromatic plants on harmful and
beneficial insects, usage areas and experiments about these plants and pests have been investigated.

KEYWORDS
plant health, pests, medicinal aromatic plant, environmentally friendly

Corresponding Author: ZHAL SATI, zhlerenler@gmail.com


1
ZRA MCADELE MERKEZ ARATIRMA ENSTTS MDRL-ANKARA

1394
Poster Session 10
Submission ID: 1349

-AMYLASE AND -GLUCOSIDASE INHIBITORY ACTIVITIES OF


THE EXTRACTS AND CONSTITUENTS OF FERULAGO
PACHYLOBA ROOTS

1 2 1 1 1 3
SONGL KARAKAYA , SEFA GZC , ZHAL GVENALP , HILAL ZBEK , HAFIZE YUCA , CAVIT KAZAZ , CEYDA
4
SIBEL KILI

ABSTRACT
Context: Ferulago species have been used since ancient times for the treatment of intestinal
worms, hemorrhoids and as tonic, digestive, aphrodisiac and sedative. Apart from its medicinal uses,
they have been used as salad or spice due to their special odors. Objectives: This study reports -
amylase and -glucosidase inhibitory activities of extracts and bioactive compounds isolated from the
roots F. pachyloba. Material and methods: The structures of isolated compounds through in vitro
bioassay-guided fractionation processes from the roots of F. pachyloba were elucidated by detailed
analyses of 1D and 2D NMR and ESI-MS data. Results: Eight known ones, osthole (1), imperatorin
(2), bergapten (3), prantschimgin (4), grandivitinol (5), xanthotoxin (6), felamidin (7), umbelliferone
(8), and a sterol mixture consisted of stigmasterol (9), -sitosterol (10) was isolated from the roots of
F. pachyloba. Felamidin and osthole showed significant -glucosidase inhibitory activity with 0.42
and 0.95 mg/mL IC50 values, respectively, when compared to the reference standard acarbose (IC50
4.95 mg/mL). On the other hand, none of the tested extracts were found to be active on -amylase
inhibition. Discussion and conclusion: The present study demonstrated that among the compounds
isolated from CH2Cl2 fraction of F. pachyloba roots, coumarins were determined the main chemical
constituents of this fraction. This study aims to give first report on isolation and characterization of the
bioactive compounds from root extracts of F. pachyloba and to report -amylase and -glucosidase
inhibitory activities of this species.

KEYWORDS
Ferulago pachyloba; Apiaceae; -glucosidase; -amylase; coumarin; felamidin; osthole

Corresponding Author: SONGL KARAKAYA, ecz-songul@hotmail.com


1
ATATRK NVERSTES ECZACILIK FAKLTES
2
ERZNCAN NVERSTES ECZACILIK FAKLTES
3
ATATRK NVERSTES FEN FAKLTES
4
ANKARA NVERSTES FEN FAKLTES

1395
Poster Session 10
Submission ID: 1351

CARDAMOM AS A FUNCTIONAL FOOD: ANTIMICROBIAL EFFECT

1 1 1 1
TESLIME ZGE YRSN , NILFER ACAR TEK , GAMZE AKBULUT , OSMAN BOZKURT

ABSTRACT
Cardamom is a tall, perennial herbaceous plants belonging to Elettaria and Amomum species
of the Zingiberaceae family. Commonly grown and used species are Elettaria cardamomum Maton
(green cardamom) and Amomum subulatum Roxburgh (black cardamom). The plants of these two
species are known for their characteristic flavor of 15-20 black-brown seeds buried in the fruit. The
sweet, spicy and citrusy flavor of cardamom is determined by the essential oil content. Volatile oil
content in seeds ranges from 2% to 5% depending on storage conditions. Essential oil contains 1,8-
cineol (36.3%), -terpinyl acetate (31.3%) and limonene (11.6%) compounds. The content of 1,8-
cineol and -terpinyl acetate is the most important factor in the formation of the aroma of cardamom.
Due to the volatile oils in its composition, the shelf life of the cardamom is short and the taste is
directly influenced by the amount of these essential oils. Cardamom gives the most aroma to the food
as powder. However, loss of fat and aroma is also rapid in this form. For this reason, it is necessary to
protect the cardamom powder more carefully than in the form of seeds and capsules. It is stated in
vacuum package that it can be stored at 5 and 90-180 days without losing aroma. As well as
flavoring of nutrients, cardamom is also used in therapeutic and preventive curative in traditional
medicine. It is generally used as a therapeutic agent in dental and gingival infections, tuberculosis,
digestive and renal diseases. Due to antimicrobial effects against foodborne bacterial pathogens,
interest in spices and aromatic herbs is increasing day by day in both industrial and scientific
researches. Bacteria can develop resistance to drugs used in the treatment of bacterial infections.
Therefore, new alternatives to these drugs need to be developed. When the antimicrobial effects of
cumin, cinnamon, black cardamom and clove plants were examined, it was found that the black
cardamom extract had the highest antimicrobial activity against Escherichia coli SS1 at the lowest
concentration (2.83 mg/ml) and because of its eucalyptol content, it possessed good antibacterial effect
on Salmonella spp. and Bacillus licheniformis. When evaluated according to extraction solvents, the
aqueous extract inhibited all bacterial strains and showed the most effective extract on Staphylococcus
aureus. Green cardamom extract was found to inhibit bacterial strains of Pseudomonas aeruginosa,
Mycobacterium smegmatis, Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli,
Salmonella typhimurium, Enterococcus faecalis, Micrococcus luteus, Candida albicans, Salmonella
typhi, Shigella flexneri and Staphylococcus aureus. The type of bacteria in which the green cardamom
extract was most effective was observed as S. aureus, an important cause of foodborne intoxication. It
was found that black cardamom was effective against Bacillus subtilis, Staphylococcus aureus,
Pseudomonas aeruginosa and Escheria Coli bacteria, and this effect on Escherichia coli and Bacillus
subtilis was more effective than erythomycin, an antibacterial drug. The green cardamom was also
effective against M. luteus, S. aureus and B. cereus bacteria after it is cooked. The use of 0.3 mg/g of
green cardamom extract with high antimicrobial efficacy has been shown to induce toxic effects in rats
by triggering inflammation, oxidative stress and cardiac cell necrosis in the brain. It is recommended

Corresponding Author: TESLME ZGE YRSN, tozgeyrsn@gmail.com


1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1396
that the amount of green cardamom be not more than 0.03mg/g. The methanolic extract of black
cardamom seed is not toxic up to 3 g/kg. As a result, cardamom is a promising plant, especially due to
antimicrobial effects on food-borne pathogens. It has been reported that high dose intake may be toxic
as a result of rat studies, but more dose studies are needed.

KEYWORDS
Cardamom, Food-borne pathogens, Functional foods, Antimicrobial effect

1397
Poster Session 10
Submission ID: 1352

DETERMINATION OF THE CURRENT SITUATION OF LAVENDER


PRODUCTION IN TURKEY BY SWOT ANALYSIS

1 2 2
SELDA ARSLAN , MERVE GZEL , AHINDE ILI

ABSTRACT
Lavender, one of the most tradable 15 essential oil plants in the world, has become the most
popular plant of the cosmetics industry in recent years. Flowers are the part of lavender which has
economic value. Essential oil is extracted from the flowers and flower stalks of the plant. According to
TURKSTAT data; 747 tons of lavender was cultivated from 570 hectares of land in 2016, in Turkey.
The cultivation of lavender and planting areas has been increased with its increased importance in
cosmetic industry. However, this increase is still not enough to satisfy the demand. For this reason,
Turkey imports essential oil extracted from lavender or lavantin, from the other countries such as
Germany, France, England, Switzerland. According to TURKSTAT data; 6 tons of lavender or
extracted essential oil, costed 192 thousand dollars, were imported in 2016, while only 194 kg of was
exported and about 20 thousand dollars was earned. In this study, the strengths, weaknesses,
opportunities and threats of lavender cultivation in Turkey have been determined. By this way, the
current situation has been put forward by considering internal and external evaluation and targeted to
bring forward proposals for reducing import. The lavender cultivation is quite suitable for the
evaluation of unplanted, barren and irrigation limited lands, when the types of plants cultivated are
considered in Turkey. For this reason, it has been pointed out the correct cultivation planning of
lavender, a perennial plant, and precautions that should be taken before entering the lavender market.
In addition that, the aim of the study is to acquire the unplanted land for agriculture and to make
agricultural fields more efficient by encouraging the cultivation of lavender as an alternative crop.

KEYWORDS
Lavender production, SWOT analysis, Essential oil, Alternative product, Import / Export

Corresponding Author: SELDA ARSLAN, selda.arslan@tarim.gov.tr


1
TARIMSAL EKONOM VE POLTKA GELTRME ENSTTS
2
TRAKYA TARIMSAL ARATIRMA ENSTTS

1398
Poster Session 10
Submission ID: 1353

PROBLEMS OF MEDICAL AND AROMATIC PLANT USE IN TURKEY

1 1 2 1
KASIM EREN TUNA , TUE UUR , NERMIN BAH , DILEK BOSTAN BUDAK

ABSTRACT
Medicinal plants are identified and used directly or indirectly in the treatment of the humans
and the animals, or are applied in order to avoid the diseases. The plants that produce and exude any
aromatic substances are so called aromatic plants. They contain aromas, flavors, condiments and
essential oils. The traditional and modern medicine utilize the medicinal and aromatic plants as
remedies to prevent diseases, to maintain health and healing. They are useful as nutritional
supplements, herbal teas, taste and flavor in feeding therewithal. In our country, the people are
interested in using herbal remedies, day by day. The exposure to intense information through the
television, internet and media has also raised the demand on medicinal and aromatic plants. It is
significant and quite important to know the origin of the medicinal herbs (i.e. where and when they are
collected) in terms of the effectiveness of the related plant in the treatment of diseases and for the
human health. While we take precautions against harmful residues accumulated on an apple bought
from the market, by washing thoroughly or peeling the crumbs before consuming it; this will not be as
possible as in dried herbs that give off their flavor and active ingredients on the water. The plants
collected from various areas where there is a high level of toxic gas emission such as neighboring
highways, railway lines and factories, in gardens, fields and grasslands where chemical fertilizer are
frequently used or sprayed, polluted waterside, etc. will also cause a risk for the human health. For
medicinal and aromatic plants, the washing (made usually for root drogs), drying and storage process
period is very important after the plant has been collected until being put to the shelves of the market.
For example, the washing process must be done with cool water and the plants must not be wait wet
before starting the drying process. Taking a long time contact with water can lead to a loss of the
active substance, as well as increased risk of spoilage. The drying process is also of great importance
for the protection of the active substance. In this case, direct contact with the sun's rays in a long-term
should be avoided. The plant should not cause any loss of active substance while the plant loses water.
On the other hand, we should also be sure that the plant has lost enough water and should not pave the
way for the formation of bacterias and fungal growth. While storing and drying the medicinal plants,
we should pay attention to the products which should not exceed the microbial activity limit because
of the moisture they can have and simultaneously the loss of the essential oils in the extreme heat will
be unfavourable as well. A dry and cool storage area should be selected. Another common problem in
our country is the unrestrained sales of these herbs on internet and the weak control of herbalists. The
seller of medicinal herbs, by other means the herbalist is "the person who prepares and sells spices,
fragrance, healing and beneficial plants". When the pharmacies were not available in old times as they
are today, the herbalists had not only sell fragrance, but also animal and vegetable plants used for
pharmaceutical production. Various precautions can be taken to solve these problems. In this paper,

Corresponding Author: DLEK BOSTAN BUDAK, dbostanbudak@gmail.com


1
UKUROVA NVERSTES
2
OSMANYE KORKUT ATA NVERSTES

1399
general problems of medical and aromatic plant use in Turkey are discussed; and some
recomandadtions have been proposed.

KEYWORDS
Medical Plant, Aromatic Plant, Ethical Problems, Turkey

1400
Poster Session 10
Submission ID: 1354

BIOACTIVE, PHARMACOLOGICAL PROPERTIES AND


THERAPEUTIC USE OF CAPPARIS PLANT

1 1 1
LALE EREZER , ASIM KART , HIDAYET TUTUN

ABSTRACT
Cappari is a plant widely grown in the hot regions of the world, belonging to the genus
capparis from the family of Capparidaceae, consisting of 39 genera and 650 species. Among these
species, Capparis spinosa, Capparis ovata and Capparis decidua are the most commonly investigated
species in terms of pharmacological, therapeutic and nutritional properties. The presence of alkaloids,
phytosterols, flavanoids and phenolic acids, glycosinolates, antioxidants, quarternary ammonium
compounds and vitamins with different pharmacological activity were found in the caper plant. The
cappari species have significant anti-inflammatory activity, which is reported to inhibit the formation
of prostaglandin and other inflammatory mediators in the cyclooxygenase pathway. Studies using
different extracts have shown that these extracts exhibit partial and selective antimicrobial activity.
The cappari plant has a very rich antioxidant structure, mainly quercetin, phenolic compounds,
glucosinolates, alkaloids, flavanoids, and routines. It has been reported that some glucosinolates such
as benzyl-, p-hydroxybenzyl-, and 2-hydroxybutyl-3-enyl glucosinolate present in the cappari plant are
chemically protective against cancers. Antidiabetic, immunomodulatory, diuretic, antiallergic,
antidiarrheal, antiviral, hepatoprotective, antiatherosclerotic, antifungal, antihypertensive,
anthelmintic, diuretic and hypolipidemic effects of different cappari species have been shown. Some
studies have shown that the plant species belonging to the family Capparidaceae also have effects on
the nervous system. In the recent years, therapeutic effects have been reported for cappari species with
sedative, antidepressant, anticonvulsant, analgesic, antiepileptic effects and for myelin loss seen in
multiple sclerosis.

KEYWORDS
Cappari, Pharmacotherapy, Bioactive components, Phytoterapi

Corresponding Author: ASIM KART, asimkart@hotmail.com


1
MEHMET AKF ERSOY NVERSTES

1401
Poster Session 10
Submission ID: 1355

THE EFFECT OF BLACK MULBERRY (MORUS NIGRA L.)SYRUP


ORAL BACTERIA ON BIOFILM FORMATION

1 1
ELIF AY , MERIH KIVAN

ABSTRACT
Black mulberry (Morus nigra L.) is a kind of flowering plant in Moraceae family, and is
unique to southwest Asia. There are wild and cultivated species of mulberries in Anatolia, which have
a significant change. The amount of mulberry production in Turkey is high. Black mulberry (M. nigra
L) is a very rich source of flavonoids, especially anthocyanins. It is stored in different forms, because
it is a non-durable product. One of them is to make a mulberry syrup. Aphtous ulcers are very painful
lesions, often surrounded by a pale yellow-red halo in the mouth on the buccal and lip mucosa, tongue,
soft palate, pharynx, and gingiva. Mulberry syrup is commonly used for the treatment of lesions in the
mouth. Microorganisms are protected by biofilm formation, from negative environmental conditions.
They can survive and multiply in the biofilms. Biofilms are resistant to environmental conditions such
as antibiotics and chemical substances. Various substances and antibiotics are used for preventing
biofilm formation. Their use also leads to various problems; so in recent years the trend towards
natural products has been increased. In this study, the effect of the mulberry syrup on biofilm
formation of bacteria that was isolated from mouths, which containing aphtous ulcers was
investigated. Previously isolated bacteria from patients mouths with aphtous ulcers were obtained
from the Department of Microbiology of the Biology Department of Anadolu University and used in
studies. The minimum inhibitory effect of mulberry syrup on oral bacteria was determined by
microtiter plate method. The effect of mulberry syrup on the formation of biofilm was investigated by
2 protocols. In the first protocol, mulberry syrup and bacteria were applied together. In the second
protocol, the mulberry syrup is added after the formation of the biofilm. Biofilm formation was
determined by microtiter plates and spectrophotometric method at 570nm with Krstal Violet dye. It
has been found that black mulberry syrup inhibits biofilm formation when it was added before biofilm
formed, but is not effective on preformed biofilm and even caused increase of the biofilm formation.
As a result; the application of mulberry syrup inhibits biofilm formation. However, it is not effective
after biofilm occurred. Furthure studies are needed especially with glucose-free preparations.

KEYWORDS
Mulberry syrup, MorusnigraL., biofilm, oral bacteria

Corresponding Author: ELF AY, elifay750@anadolu.edu.tr


1
ANADOLU NVERSTES

1402
Poster Session 10
Submission ID: 1356

INVESTIGATION OF CYTOTOXIC AND GENOTOXIC EFFECT OF


DR.HONEYWAX HEROVIC FOOD SUPPLEMENT IN VITRO

1 1 1 1 2
NILFER INKILI , TOLGA AVA , ZGR VATAN , HUZEYFE HURIYET , HASAN AHINOLU

ABSTRACT
Dr.HONEYWAX Herovic is a liquid form food supplement herbal product. This syrup
product includes; propolis, kalonji, daisy, rosehip extracts, apple concentrate and honey. In the present
study, we evaluated the potential cytotoxic and genotoxic activity of the Dr.HONEYWAX Herovic on
human healthy lung epithelial cells Beas-2B. Potential cytotoxic effect was tested by XTT assay and
potential genotoxic effect was tested by comet assay. Cells were treated with serial concentrations of
Dr.HONEYWAX Herovic (0,142; 1,42; 14,2; 142; 1420 ppm) for 24h in XTT assay. Any cytotoxic
effect was not identified even at dose 1420 ppm, which is ten thousand times upper from the dose of
0,142 ppm, which is the in vitro adaptation of recommended human daily therapeutic dose by XTT
assay. Cells were treated with three doses of Dr.HONEYWAX Herovic (0,142; 142; 1420 ppm) for
24h in comet assay. Tail length, % Tail DNA and Olive Tail Moment parameters were evaluated in
comet assay. Any genotoxic effect was not identified even at highest dose of 1420 ppm.
Dr.HONEYWAX Herovic, which is a food supplement did not show any cytotoxic and genotoxic
effect even at highest dose, which is ten thousand times from recommended human daily dose by XTT
and comet assay on Beas-2B cells.

KEYWORDS
Herbal extract, herbal food supplement, propolis, honey, cytotoxicity, genotoxicity, human
healthy fibroblast cell line

Corresponding Author: NLFER NKILI, aydemirn@uludag.edu.tr


1
ULUDA NVERSTES FEN EDEBYAT FAKLTES BYOLOJ BLM GRKLE KAMPS BURSA
2
HNKAR NATUREL BTK LOKMAN BURSA

1403
Poster Session 10
Submission ID: 1357

ALOE VERA: A MIRACLE PLANT WITH ITS WIDE-RANGING


APPLICATIONS

1 1 1
KHAZINA AMIN , SENAY OZGEN , ZELIHA SELAMOGLU

ABSTRACT
Aloe vera, a plant of Liliaceae family, is not less than a blessing from centuries to date. It is
native of hot and arid regions and widely grown as an ornamental plant. It has become a common
household remedy for a variety of uses. This plant is also well known as medicinal herb. The leaves of
plant contain mucilage tissue or aloe gel that is used for cosmetics and to cure mankinds ailments.
Aloe vera has marvelous herbal remedy with defined clinical effectiveness. The active chemical
constituents of Aloe vera include essential amino acids, anthraquinones, enzymes, minerals, vitamins,
lignins, sugars, salicylic acid, folic acid, saponins, and sterols. There seems to be no single magic
ingredient, all the nutritional ingredients work together in a synergistic way to create healing action
and other health benefits. Aloe vera plays a role as complementary medicine to heal cutaneous
wounds, burnings, and infections. Health promoting benefits of aloe vera are due to its anti-
inflammatory, antibacterial, antifungal antiarthritis and hypoglycemic effects. Due to these marvelous
attributes, this plant has the ability to reverse ailments and soothe human life in a myriad ways. With
the gaining traction as an important ingredient in cosmetics, food and pharmaceuticals, many countries
have started growing it commercially. Aloe vera farming is a very promising activity. There is a need
to encourage Aloe vera farming as an alternative traditional crop to meet market demands. Efficient
production technology along with well-established marketing channels for the distribution of leaves to
processing units at a fair producer price is required for its economic sustainability.

KEYWORDS
anthraquinones, folic acid, saponins, sterols

Corresponding Author: KHAZNA AMN, khazinaamin@gmail.com


1
MER HALSDEMR UNVERSTY

1404
Poster Session 10
Submission ID: 1358

MELATONIN ADSORPTION WITH L-TRYPTOPHAN GRAFTED


POLY(HEMA) MEMBRANES FROM MEDICAL SEEDS

1 2
TLDEN INANAN , NALAN TUZMEN

ABSTRACT
Melatonin (N-acetyl-5-methoxytryptamine) is an indoleamine synthesized from tryptophan
which an essential amino acid. It is involved in the regulation of circadian rhythm (sleepwake cycle)
and the alleviation of insomnia arised from jetlag and shift work. Melatonin minimalizes
neurodegenerative diseases, such as Alzheimers and Parkinsons diseases and also acts as an
anticancer agent. Also, melatonin may be used as a protective agent against to ocular diseases and
headache disorders. In addition, potential antioxidative properties and anti-inflammatory effects of
melatonin have been reported. Melatonin is widely distributed in the animal kingdom. High levels of
melatonin have also been detected in several medicinal herbs and in the seeds of edible plants.
Numerous studies have been reported about the presence of melatonin in plants with a wide range of
concentrations from picograms to micrograms per gram of plant tissue. Due to their common usage in
medicine and cosmetic industry, extraction and determination of melatonin selectively and with high
purity using rapid and low-cost method from natural sources, such as high plants, have great
importance. Radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), high
performance liquid chromatography (HPLC), and gas chromatography-mass spectroscopy (GC-MS)
have been used for quantitative melatonin determination. The different methods such as liquid-liquid
extraction, solid phase extraction (SPE) sorbents, microextraction by packed sorbent (MEPS) and
combination of these methods were used for melatonin extraction. In recent times, affinity sorbents
prepared by modification with several groups and methods draw attention for extraction of different
biomolecules from higher plants. In the current study, L-tryptophan grafted poly(HEMA) membranes
were used for melatonin adsorption from the extracts of medical seeds. Poly(HEMA) membranes were
prepared by UV photopolymerization method and graft procedure was applied in the presence of L-
tryptophan and sodium hydride. L-tryptophan grafted poly(HEMA) membranes were characterized by
several methods and then, used in melatonin adsorption. Seeds of fenugreek (Trigonella foenum-
graecum), fennel (Foeniculum vulgare), coriander (Coriandrum sativum), green cardoamom (Elettaria
cardamomum), flax (Linum usitatissimum) and sunflower (Helianthus annuus) were used for the
extraction of melatonin with 3 different extraction solution. These commercially available seeds were
obtained from herbalist in a grinded form. Each seed was extracted with ethyl acetate (EtOAC),
EtOAc:MeOH (70:30) and EtOH, separately and used in melatonin adsorption studies. Melatonin
amounts in the initial and final solutions of adsorption were determined by HPLC method with
fluorescence detection. The results have shown that melatonin adsorption was performed with high
recoveries (higher than 95 %) from all the extraction solutions of medical seeds.

Corresponding Author: TLDEN INANAN, tkalburcu@gmail.com


1
AKSARAY UNVERSTY
2
DOKUZ EYLL UNVERSTY

1405
KEYWORDS
Melatonin, adsorption, medical seeds, extraction, membrane.

1406
Poster Session 10
Submission ID: 1360

EVALUATION OF CYTOTOXIC AND GENOTOXIC EFFECT OF


SCORZONERA VERATRIFOLIA

1 1 1 1 1
AYFER BECEREN , MERT GEIM , OZLEM BINGL ZAKPINAR , TURGUT TAKIN , AHMET DOAN , SEMRA
1
ARDA

ABSTRACT
Primer and secondary metabolites of the natural products produced by the plants are the most
basic products for drug candidates that can be used directly or indirectly in medicine today. Therefore,
the number of scientific researches carried out on these compounds seems to increase day by day. In
recent years people prefer to use natural antioxidants in their daily lives in order to prevent diseases
and strengthen their immune systems. The Scorzonera genus (Asteraceae) is present by 39 species in
Turkey and 17 of them are endemic, such as a perennial herbaceous plant Scorzonera veratrifolia. This
plant is distributed in Eastern Anatolia and grows on dry rocky hillsides. Recent studies showed that
Scorzonera species contain dihydroisoquinarine, bibenzyl derivatives, flavonoids, lignans, stilbene
derivatives, quinic and caffeic acid derivatives, sesquiterpene, sesquiterpene lactones and triterpene
compounds. Studies have shown that Scorzonera species exhibit antioxidant, analgesic, anti-
inflammatory and wound healing activity. Although Scorzonera veratrifolia is widely used as a natural
product in traditional medicine, up to our knowledge no study in the literature has been demonstrated
until now. Therefore, the aim of the present study was to evaluate the possible genotoxic effect of
Scorzonera veratrifolia in wistar albino rats by comet assay and cytotoxic effect on human colon
cancer cell line HT-29, human lung cancer cell line A549, human cervical cancer cell line HeLa and
human prostate cancer cell line PC-3 by MTT method. Wound model with incisional wounds was
made on wistar albino rats and the extracts were applied for 10 days. At the end of the treatment,
whole blood was collected to determine the genotoxic potency by using Comet assay. The cytotoxic
effect of extracts at a dose of 100 micrograms on the proliferation of cancer cells was determined by
MTT method on different human cancer cell lines. According to our results, heptan and methanol
extracts from the aerial parts and roots of Scorzonera veratrifolia did not show any genotoxic effect in
vivo model system, however, it was determined that no inhibition was observed on the cancer cell
proliferation, in contrast the cancer cells were proliferated. This proliferative effect was found to be
more significant on cervical, colon and prostate cancer cell lines. Therefore, the findings of this study
recommend that Scorzonera veratrifolia plant should not be consumed especially in cancer patients
because of its positive effects on cell proliferation and further studies are needed to highlight this
important traditional plant.

KEYWORDS
Scorzonera veratrifolia, genotoxicity, cytotoxicity, Comet assay, MTT method, cancer cell
lines

Corresponding Author: AYFER BECEREN, ayfer.tozan@marmara.edu.tr


1
MARMARA NVERSTES

1407
Poster Session 10
Submission ID: 1361

ANTIBIOFILM EFFECT OF DIFFERENT ESSENTIAL OILS IN


P.AERUGINOSA PAO1

1 2
EBRU NEM , RAMADAN BILGIN AKALIN

ABSTRACT
Biofilm is the life form of microbes attached to surfaces, which might be found in somewhere
like medical, industrial and natural surfaces. Bacteria compose biofilm use the system which is named
quorum sensing (QS) and it based on density-dependent cell to cell communication. In biofilm form,
bacteria are more resistant to antimicrobial treatments. So new researches focus on blockage of this
form and some chemical compounds and also plant extract have used for this aim. In this paper eight
different commercial essential oils (Amyris balsamifera, Nerium oleander, Ormenis multicaulis, Citrus
grandis peel, Citrus nobulis, Citrus nobilis, Styrax, Citrus sinensis) have been evaluated inhibition of
biofilm formation against reference strain Pseudomonas aeruginosa PAO1. Inhibition effect of oils on
biofilm was searched cristal viole (CV) assay. Biofilms were grown on LB medium in 96-well
polystyrene plates in the presence and absence of 4 different concentrations of the oils. All tests
repeated 3 times and evaluated for statistical. According to the result of this study significant reduction
of biofilm composition were seen first concentration of Citrus nobilis, Citrus sinensis, Styrax.
Inhibition rates are respectively 97%, 98%, 91%. The first concentration of Nerium oleander and
Ormenis multicaulis showed antibacterial effect on P. aeruginosa PAO1. Citrus grandis peel and
Amyris balsamifera showed low and also antibiofilm effect(25%, 17%). And other concentration of
plant oils have different antibiofilm rate.

KEYWORDS
Biofilm, P. aeruginosa PAO1, essential oil

Corresponding Author: EBRU NEM, ebruonem@sdu.edu.tr


1
SLEYMAN DEMREL NVERSTES
2
NAMIK KEMAL NVERSTES

1408
Poster Session 10
Submission ID: 1363

IMPORTANCE OF BERBERIS CRATAEGINA DC (HAWTHORN


BARBERRY ) FOR FOOD

1 1 2 1
BAYRAM YURT , LTFI BEHET , MER BEYHAN , NURULLAH DEMR

ABSTRACT
Importance of Berberis crataegina DC for food Turkey is located in a region that has rich plant
variety among the major regions of the World. A significant part of Turkey flora (1/3) is endemic to
Turkey. Unfortunately, this richness of plant is not well exploited and the knowledge about wild plants
is limited (especially in the application fields). We just have knowledge about chemical composition
and usage culture of limited number of plants. The members of Berberis L. genus within
Berberidaceae family are known as hawthorn barberry in Turkey. This genus includes four members
(type) in Turkey. Berberis crataegina is common in Turkey and some countries around the world.
Berberis crataegina is found in Inner Western Anatolia, upper sakarya, Konya province, Middle
Kzlrmak region, Antalya region, Dicle and upside of Frat Basin. Fruits of Berberis crataegina
species are used for different purposes in Turkey. Fruits of Berberis crataegina have high antioxidant
activity. Antioxidants are very important for health and have many important functions such as
antimutagenic, anticarcinogenic and antiaging. Researches demonstrate that this natural wild plant
possesses antitumor, antibacterial activity and positive effects on urinary tract disorders. Fruits of
Berberis crataegina are generally collected in July-August, depending on climate conditions in Turkey.
In early July, the fruit has sour taste and light purple but in ripening stage it turns to sweet taste and
dark purple (August). Collected fruits are used for making home-made fruit juice, marmalade and
jelly. The fruits of the hawthorn barberry are also could be consumed as tea. In addition, Berberis
crataegina fruits are dried with sun and form daily diet of the local people as snack foods. Fruits of
Berberis crataegina used in the production of ice cream are highly appreciated by the consumers
because of the natural taste, aroma and color especially when added to ice cream thanks to its natural
dark purple color. The greatest benefit of this wild plant is its potential use as natural color additive
(dark purple color) in food products.

KEYWORDS
Berberis crataegina DC, hawthorn barberry, antioxidants, natural color additive

Corresponding Author: BAYRAM YURT, bayramyurt@gmail.com


1
BNGL NVERSTES
2
SAKARYA NVERSTES

1409
Poster Session 10
Submission ID: 1365

ANTIOXIDANT ACTIVITY OF ACHILLEA CAPPADOCICA

1 1 1 2
NUSRET GEN , HSEYIN AKT , MAHFUZ ELMASTA , ZEYNEP AKT

ABSTRACT
Antioxidants can inhibit or delay the oxidation process in living organism and food stuff. The
intake of naturally occurring antioxidants such phenolics and vitamins can play important role to
fighting free radicals. A great interest came out to finding new natural antioxidants from natural
sources to replace synthetic ones due to restricted side effects. The greatest source of natural
antioxidants is medicinal aromatic plants. Plants have been used for a large range of purposes
including medicine, nutrition, flavorings, fragrance and industrial uses. The aim of this study was to
investigate antioxidant properties of Achillea cappadocica. Plant materials were collected from
Refahiye, Erzincan at June 2016. Floral parts were separated and dried. In order to determine of
antioxidant activity of A. cappadocica, two extraction systems were used. Floral parts of plant material
were boiled in water for 30 min to mimic preparation aromatic plant decoction in folk medicine. The
plant residue was removed by filtration. Filtrate dried using a lab-scale lyophilizator to give light
yellow powder (Extract I). A methanol extract was prepared and suspended in hot water. The insoluble
parts (mostly contain chlorophylls) were removed using filter paper. A small amount of filtrate dried
as above to give dark yellow powder (Extract II). In order to remove water soluble carbohydrates and
concentrate biologically active components, water phase partitioned with ethyl acetate and butanol in
separation funnel respectively. Organic layers were concentrated to dryness to give Extract III (etOAc
phase) and IV (butOH phase). The remained water layer after partition was lyophilized (Extract V).
Total phenolic and flavonoid content of five extracts were determined using spectrophotometric
methods. Antioxidant capacity of A. cappadocica was evaluated using DPPH and ABTS radical
scavenging activity and reducing power tests. Extract III was found both phenolic and flavonoid rich
extract (119.9 and 71.46 mg/g extract respectively). Also this extract was found most active when
compared others. Extract V was found poor both active compound content and activity. Extract III
contains 60 fold more phenolic compounds than Extract V. It means the partition process was success
to extraction of phenolics from water extract. DPPH and ABTS radical cation assays, expressed as
IC50 value (g/mL), were used for evaluation of radical-scavenging properties of obtained five
extracts from plant material. DPPH radical scavenging ability of extracts calculated 18.84 g/mL for
Extract III and 133.40 g/mL for Extract V. The reducing power of most active extract was found
369.22 mol trolox equivalent activity/g extract.

KEYWORDS
Achillea cappadocica, antioxdant activity

Corresponding Author: NUSRET GEN, nusretgenc@gmail.com


1
GAZOSMANPAA NVERSTES
2
GMHANE NVERSTES

1410
Poster Session 10
Submission ID: 1366

TURKEY'S WILD ORCHIDS AND MARAS ICE CREAM

1 2 1
NAZLI TRKMEN , ZGE KINDIR UURLUOLU , AYE GRSOY

ABSTRACT
There are 10 different breeds and 80 different species of wild orchids in Turkey. An important
part of these plants belonging to the Orchidaceae family is also endemic. Due to threats such as
industrialization, urbanization, extension of agricultural areas, overgrazing and tourism activities, wild
orchid species that grow in Turkey are threatened with extinction. The main reason why wild orchids
are collected from nature is their use in the production of salep. The salep obtained by drying and
milling the tubers of these plants is mostly used in the production of Maras ice cream in our country.
Maras ice cream is a traditional product produced in Kahramanmaras and consumed lovingly which is
sold to various parts of the country and even abroad. It is known that the unique structure and flavor of
Maras ice cream originated from the goat milk used in its production and especially from the wild
orchids grown in the mountains. At least 10-20 million wild orchids are removed from nature every
year for Salep production (Sandal and St, 2010). In order to obtain 1 kg of salep, approximately
1000-4000 tubers, ranging from 0.25 to 1 gram, are needed (Tekinen and Gner, 2010). Thus,
although the collection of wild orchids from nature is prohibited and serious sanctions are imposed on
unauthorized gatherers, millions of wild orchid individuals are annihilated from the nature each year
for the salep production. Due to the fact that the mentioned problem is still not fully resolved, various
studies have been carried out on the reproduction of these species. Within the scope of the "Salep
Action Plan" carried out by the General Directorate of Forestry of the Republic of Turkey Ministry of
Forestry and Water Affairs; studies on the propagation of wild orchids in the natural environment,
establishment of gene-source seed gardens, expansion of the spreading areas by transferring wild
orchid members raised in nurseries to their natural distribution areas and preservation of wild orchids
in their natural environment are still continuing. As a result of researches on the propagation of wild
orchid members in the culture medium, it was determined that Serapias vomeracea (Burm. fill.) Brig,
Orchis sancta L. and Ophrys bombylifera Willd. were the most prominent species in terms of their
tubing ability, and the first two of these species were mentioned in terms of adaptation to culture
conditions and widespread availability. Orchis morio L., Orchis italica Poiret. and Orchis anatolica
Boiss. species were reported to have other promising species (Anonymous, 2014). Although it is
generally accepted that salep from each species of wild orchid is not suitable for ice cream production,
it is still unclear which species are suitable. Salep is known as a product having a high content of
glucomannan, depending on the wild orchid species obtained, and the salep used in the production of
ice cream is generally selected to have the highest content of glucomannan. Therefore, the salep
obtained from any wild orchid species collected from nature without permission can not be used in the
production of ice cream. It is thought that many wild orchids can be prevented from being collected
from nature by the determination of wild orchid species suitable for use in the production of ice cream
and the emphasis on studies on the reproduction of these species in the culture medium.

Corresponding Author: NAZLI TRKMEN, nazli.turkmen@ankara.edu.tr


1
ANKARA NVERSTES
2
T.C. ORMAN VE SU LER BAKANLII ORMAN GENEL MDRL

1411
KEYWORDS
Wild orchid, salep, Maras ice cream

1412
Poster Session 10
Submission ID: 1369

DETERMINATION OF ANTIOXIDANT PROPERTIES OF RUMEX


CRISPUS AND SCROPHULARIA CANINA SUBSP. BICOLOR

1 1 1 1 1
SERHAT DEMIR , BUKET BOZKURT , MUSTAFA ALI NR , GLEN IREM KAYA , NEHIR NVER SOMER

ABSTRACT
Determination of Antioxidant Properties of Rumex crispus and Scrophularia canina subsp.
bicolor Demir S.1, Bozkurt B.1, nr M.A.1, Kaya G..1, nver-Somer N.1 Department of
Pharmacognosy, Faculty of Pharmacy, Ege University, 35100, zmir The genus Rumex L.
(Polygonaceae) consists of about 200 species growing worldwide and 23 species and 5 hybrids
naturally growing in Turkey (1,2) Rumex roots have important traditional uses in relation to their
laxative properties. Decoctions prepared from the underground parts have been claimed to be
therapeutically useful as cholagogue, tonic and laxative and for blood cleansing. Fresh leaves are used
to treat eczema and also consumed as vegetable in Anatolia (3). In the flora of Turkey, Scrophularia is
represented by 59 species, 23 of which are endemic. Some Scrophularia L. species, especially S.
nodosa L. are used in folk medicine as a diuretic and for the treatment of wounds and hemorrhoids
(4,5). Different species of the genus Scrophularia (Scrophulariaceae) have been used in traditional
medicine to treat some diseases, including dermatosis and inflammatory affections (6). In the present
study, Rumex crispus L. and Scrophularia canina L. subsp. bicolor (SM.) Greuter collected from
Soma, Manisa, were investigated for their antioxidant activity using the DPPH method. Methanol and
ethyl acetate extracts were prepared from the above-ground parts of these plants. Significant
antioxidant activity was determined for methanol (IC50: 4.16 g/mL) and ethyl acetate (IC50: 8.71
g/mL) extracts of Rumex crispus L. Moreover, methanol (IC50: 60.78 g/mL) and ethyl acetate
(IC50: 149.33 g/mL) extracts of Scrophularia canina subsp. bicolor (SM.) Greuter were shown to
have important free radical scavenging antioxidant activity. Key words: Antioxidant activity, Rumex
crispus, Scrophularia canina subsp. bicolor References 1. Cullen J. 1967. Rumex L. in Davis P.H.
(ED). Flora of Turkey and East Aegean Islands Edinburgh: University Press. 2: 281293. 2. Baytop T.
1963. Medicinal and Poisonous Plants of Turkey. Akgn Press, University of Istanbul, 315. 3. Baytop
T. 1999. Therapy with Medicinal Plants in Turkey (Past and Present). Nobel Tp Kitabevleri, 282-283.
4. Lall SS., Mill RR. 1978. Scrophularia L. In Davis P.H. (ED). Flora of Turkey and the East Aegean
Islands. Edinburgh University Press. 6: 603-647. 5. al I., Sezgin Y., Dnmez AA., Redi P.,
Tasdemir D. 2007. Journal of Natural Products 70:1, 43-47. 6. Fernandez MA., Garcia MD., Saenz
MT. 1996. Antibacterial activity of the phenolic acids fractions of Scrophularia frutescens and
Scrophularia sambucifolia. Journal of Ethnopharmacology 53: 11-14.

KEYWORDS
Antioxidant activity, Rumex crispus, Scrophularia canina subsp. bicolor

Corresponding Author: SERHAT DEMR, eczserhatdemir@gmail.com


1
EGE NVERSTES ECZACILIK FAKLTES FARMAKOGNOZ ANABLM DALI

1413
Poster Session 10
Submission ID: 1370

INVESTIGATION OF CYTOTOXIC AND GENOTOXIC EFFECT OF


DR.HONEYWAX VEROX FOOD SUPLEMENT

1 1 1 1 2
TOLGA AVA , NILFER INKILI , ZGR VATAN , HUZEYFE HURIYET , HASAN AHINOLU

ABSTRACT
Dr.HONEYWAX Verox is a food supplement product. These capsule product includes;
grounded Thistle, Carop, Ginger, Turmeric, pollen and Cinchona. In the present study, we evaluated
the potential cytotoxic and genotoxic activity of the Dr.HONEYWAX Verox on human healthy lung
epithelial cells Beas-2B. Potential cytotoxic effect was tested by XTT assay and potential genotoxic
effect was tested by comet assay. Cells were treated with serial concentrations of Dr.HONEYWAX
Verox (0,285; 2,85; 28,5; 285; 2850 ppm) for 24h in XTT assay. Any cytotoxic effect was not
identified even at dose 2850 ppm, which is ten thousand times upper from the dose of 0.285 ppm,
which is the in vitro adaptation of recommended daily human dosage by XTT assay. Cells were
treated with three doses of Dr.HONEYWAX Verox (0,285; 285; 2850 ppm) for 24h in comet assay.
Tail length, % Tail DNA and Olive Tail Moment parameters were evaluated in comet assay. Any
genotoxic effect was not identified even at highest dose 2850 ppm. Dr.HONEYWAX Verox, which is
a food supplement did not show any cytotoxic and genotoxic effect even at highest dose, which is ten
thousand times the dose of recommended human daily therapeutic dose by XTT and comet assay on
Beas-2B cells.

KEYWORDS
herbal food supplement, Thistle, Carop, Ginger, cytotoxicity, genotoxicity, human healthy
fibroblast cell line

Corresponding Author: NLFER NKILI, aydemirn@uludag.edu.tr


1
ULUDA NVERSTES FEN EDEBYAT FAKLTES BYOLOJ BLM GRKLE KAMPS BURSA
2
HNKAR NATUREL BTK LOKMAN BURSA

1414
Poster Session 10
Submission ID: 1372

EFFECT OF OREGANO ESSENTIAL OIL ON THE QUALITY OF


SUCUK

1 1 1
SONGL AHIN ERCAN , HSEYIN BOZKURT , IDEM SOYSAL

D
ABSTRACT
This study aimed to evaluate effect of oregano essential oil at concentration of 0.5% on pH,

TE
TBARS, overall sensory quality, colour (Hunter L, a, b, YI) of sucuk during the fermentation period.
A sharp decrease (P < 0.05) in pH values were observed from 5.1 to about 4.3 during the rst 3 days of
fermentation. Control and 0.5% oregano oil added sucuks showed similarity in pH change and it
reached to 6.8 at the end of the fermentation. Addition of oregano oil decreased (P < 0.05) the TBARS
value. TBARS values increased from 0.29 mg/kg and 0.52 mg/kg to 1.0 mg/kg and 1.56 mg/kg for
EN
oregano oil added and control sucuks, respectively. Hunter L, b, YI values were a ected both (P <
0.05) by fermentation period and sample, however, a-values changed significantly (P<0.05) by
fermentation period and was not affected significantly by sample (P > 0.05). Addition of oregano oil
did not signicantly (P > 0.05) affect the overall sensory quality. Overall sensory quality evaluated
from color, avor and ease of cutting scores increased (P < 0.05) from 5.7 to about 7.5.
ES

KEYWORDS
PR

oregano oil, pH, sucuk, TBARS


T
O
N

Corresponding Author: SONGL AHN ERCAN, sosahin@gantep.edu.tr


1
DEPARTMENT OF FOOD ENGNEERNG, FACULTY OF ENGNEERNG, GAZANTEP UNVERSTY,
GAZANTEP 27310, TURKEY

1415
Poster Session 10
Submission ID: 1373

RAPID QUANTITATIVE DETERMINATION OF CUMIN


ADULTERATION WITH CORIANDER AND PEANUT HULL POWDER
USING THE DSC TECHNIQUE

1 1 1
HSAN BURAK AM , HANDAN BANAL GLMEZ , AYHAN TOPUZ

ABSTRACT
Cumin (Cuminum cyminum L.) is a flowering and annual plant of the family Apiaceae.
Cumin powder is used to give flavor to foods and also has different medicinal properties. The most
common adulterants are coriander and peanut hull since they are cheap ingredients. The similarities
and differences in glass transition (Tg) and melting transition of cumin powder and adulteration
materials were investigated. Tg values and melting transition of the powders were determined by
differential scanning calorimetry (DSC). In the study, cumin, coriander powder, and peanut hull were
collected from the local market and peanut hull was grinded prior to analysis. The coriander powder
and peanut hull were blended individually with cumin powder at the ratios of 0/100
(adulterant:cumin), 30/70, 50/50, and 100/0. Mixing was on a weight basis. The DSC curves were
obtained in the temperature range from -20 to 260C under the dynamic atmosphere of N2 (25
mL/min) heating rate of =10C/min using an Al capsule containing approximately 25 mg of each
sample. The obtained result indicated that, Tg values of the cumin, coriander, and peanut hull powders
were 26.54, 77.81 and 56.79C, respectively. Additionally, melting heat value of the cumin powder
were found to be 100.94 J/g and the values of the other samples were 77.81 and 89.76 J/g. The results
of this study suggest that the DSC analysis may be available to determine adulteration of cumin with
coriander and peanut hull powder.

KEYWORDS
Cuminum cyminum L., coriander, peanut hull, adulteration, DSC

Corresponding Author: HSAN BURAK AM, burakcam@akdeniz.edu.tr


1
AKDENZ NVERSTES

1416
Poster Session 10
Submission ID: 1374

IN VITRO ANTIMICROBIAL ACTIVITY OF ARTEMISIA


ABSINTHIUM ESSENTAL OIL

1 1 1 2 3
OZGUR CELEBI , ICLAL AGAN , ULKU ALTOPARLAK , SABAN KORDALI , DEMET CELEBI

ABSTRACT
BACKGROUND The genus Artemisia has been utilized worldwide due to its immense
potential for protection against various diseases, especially wound infections. Camphor is one of the
main components of Artemisia absinthium oil. OBJECTVE This study aimed to evaluate the
antimicrobial activity of Camphor extract of Artemisia hortensis oil on microorganisms isolated from
wound infections by disc diffusion and microdilution method. MATERIAL and METHOD Artemisia
absinthium oils were isolated by hydrodistilation method. The oil composition was analyzed by Gas
Chromatography - Mass Spectrometry (GC-MS). Microorganisms were provided by Ataturk
University Research Hospital. Microdilution and Disc Diffusion Method were used to determine the
antimicrobial activity of the extract against nine wound infection agents including Pseudomonas
aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus epidermidis, Acinetobacter
baumannii, Staphylococcus aureus, Enterobacter aerogenes, Candida albicans and Escherichia coli.
RESULTS The minimal inhibition concentration (MIC) values (g /ml) were determined for P.
aeruginosa (MIC = 500 g/ml) for Klebsiella pneumoniae (MIC = 500g/ml), P. mirabilis, (MIC =
500 g/ml), S. epidermidis ( MIC < 1.95 g/ml), A. baumannii (MIC = 500 g/ml), S. aureus (MIC
<125 g/ml), E. aerogenes (MIC = 500g/ml), E .coli (MIC = 500 g/ml), C. albicans (MIC =
500g/ml). Anti-microbial activity of the camphor extract of essential oil was tested by the disc
diffusion method. The inhibition zones were measured as 18 mm for P. aeruginosa, 22 mm for K.
pneumoniae, 30 mm for P. mirabilis, 30 mm for S. epidermidis, 28 mm for A. baumannii, 30 mm for
S. aureus, 18 mm for E. aerogenes, 30 mm for C. albicans and 21 mm for E. coli. CONCLUSION
Camphor extract of A. absinthium oil showed antimibrobial effects on tested microorganisms by disc
diffusion method. The MIC values of only two microorganisms tested were different. The MIC value
of S. epidermidis (MIC < 1.95 g/ml) and S. aureus (MIC < 125 g/ml), while the others were
measured (MIC = 500g/ml) by microdulition method. Plant extracts should be considered when used
in part of replacement treatment.

KEYWORDS
Artemisia absinthium, Antimicrobial Activity, Wound Infection

Corresponding Author: OZGUR CELEB, ozdrtr@yahoo.com


1
ATATURK UNVERSTY, SCHOOL OF MEDCNE, DEPARTMENT OF MEDCAL MCROBOLOGY, ERZURUM
2
ATATURK UNVERSTY, FACULTY OF AGRCULTURE, DEPARTMENT OF PLANT PROTECTON, ERZURUM
3
ATATURK UNVERSTY, VETERNARY FACULTY, ERZURUM

1417
Poster Session 11
Submission ID: 1375

FATTY ACID COMPOSITION, STEROL, TOCOPHEROL AND


AMINOACID CONTENTS OF TRIGONELLA CARIENSIS BOISS.

1 1
ERIFE SELMA URAS GNGR , GAMZE KKDL

ABSTRACT
The genus Trigonella L. (Leguminosae) includes about 135 species distributed from the
Mediterranean regions, Southeastern Europe, Western Asia, North and South Africa (1,2). Trigonella
foenum-graecum L. is the most widely used species in Trigonella genus and it is an annual herbaceous
plant commonly called fenugreek; its seeds are used as food crops in India, to supplement wheat and
maize flour for bread making in Egypt, and as one of the staple foods in Yemen. It is credited with
many medicinal properties and is one of the oldest medicinal plants used in many Asian and African
countries. Its seeds have been used as a carminative, tonic, aphrodisiac in Ayurvedic, Chinese and
Unani systems of medicine. It was known that the plant contain flavonoids, alkaloids, saponins, fixed
oil, polysaccharides, minerals and proteins. The seeds used in many traditional systems as aromatic,
carminative, galactogogue, antibacterial, antidiabetic, hypocholesterolemic, diuretic and analgesic
agent (1-3). In Turkey, the genus Trigonella represented by 54 taxa (4,5). T. cariensis is one of these
taxa, which grows West and Soth West Anatolia, Greece and East Mediterranean area. T. foenum-
graecum has been extensively studied but there is little information about other species of the genus in
the literature (6,7). T. cariensis Boiss. have not been studied phytochemically. The aim of the present
study was to determine fatty acid compositions, sterol, tocopherol and aminoacid contents of T.
cariensis. The seeds contain 2.010.12 g/100 g fixed oil. Linoleic acid (43.740.24 %) and -linolenic
acid (18.380.45 %) along with palmitic acid (14.160.38 %) were the main fatty acids. The total
sterol content was 2247.090.06 mg/100 g, which consisted mainly of -sitosterol (62.651.53 %). -
tocopherol (233.540.47 mg/100 g) was the dominant tocopherol. L-Glutamic acid (58010.32
mg/100 g) was the main aminoacid. The results of the present study revealed that this species is
important source of essential fatty acids, tocopherols and aminoacids. The oil rich in polyunsaturated
fatty acids which play an important role in human health. Further studies are needed to evaluation of
the plant in food industry and in health. This study was supported by the Research Fund of Mersin
University in Turkey with Project Number: 2016-1-AP2-1412 References 1. Evans, W. C., Trease
and Evans Pharmacognosy. 15th. Ed., UK: University of Nottingham, 2002, 26. 2. Bown, D.,
Encyclopedia of Herbs&Their Uses. First Ed., London: Darling Kindersley Limited, 2002, 393. 3.
Srinivasan, K., Fenugreek (Trigonella foenum-graecum): A review of health beneficial physiological
effects. Food Reviews International 2006, 22, 203-224. 4. Huber-Morath A. Trigonella L. In Davis PH
(ed.) Flora of Turkey and the East Aegean Islands. Edinburgh University Pres., 1970; 3: 452482. 5.
Gokturk, R. S., A new subspecies Trigonella coerulescens (Fabaceae), from Turkey, Ann. Bot. Fenn.
2009, 46, 62-64. 6. Uras-Gungor S. S.; Guzel, S.; Ilcim, A.; Kokdil, G., Total Phenolic and Flavonoid
Content, Mineral Composition and Antioxidant Potential of Trigonella monspeliaca. Turk J Pharm Sci
2014, 11(3), 255-262. 7. Uras Gungor S. S.; Ilcim, A.; Kokdil, G., A Comparison of Diosgenin,

Corresponding Author: ERFE SELMA URAS GNGR, urasselma@hotmail.com


1
MERSN NVERSTES, ECZACILIK FAKLTES, FARMAKOGNOZ ANABLM DALI

1418
Phenolics, Fatty Acid Profiles and Mineral Contents with Free Radical Scavenging Activity of
Trigonella L. Species from Section Cylindricae. Rec. Nat. Prod. 2017, 11(1), 17-30.

KEYWORDS
Trigonella cariensis; fatty acid; sterol; tocopherol; aminoacid

1419
Poster Session 11
Submission ID: 1376

BEEKEEPING ACTIVITIES IN MEDICAL AND AROMATIC PLANT


PRODUCTION

1 1 1
ALI KEMALI ZUUR , H. AYLA SARI , ALI GNC

ABSTRACT
It was first described by the German Koelreuter and Sprengel in the years 1750-1800 that the
bees were pollinators of plants. With this special feature of bees, beekeeping activities are now used
not only for obtaining bee products but also for pollinating plants which are important for humanity.
According to the information in the literature, it is stated that 82 cultivated plants are used for a large
part of human food, 63 of these plants are pollination. Stated that 80% of the plants cultivated in
Europe need to be pollinated by bees and some other creatures. Bees make pollunate that it has been
an important source of income for beekeepers in the United States. In our country, 75% of the
beekeeping activities are carried out in the form of "bee colonies", which are called migratory
(traveler), to be kept in different regions in order to be injured by differences in flowering period
between regions. This activity naturally also provides for pollination of the plants in the areas where
the colon is left. It is seen how important beekeeping activities are for the natural equilibrium when it
is thought that a bee circulates on average 1000 flowers per day. The production of medicinal and
aromatic plants in our country is done in the form of collecting naturally grown plants. Cultural
aquaculture sites for medical and aromatic plant cultivation have been opened with new legislation and
incentives. Beekeeping activities are indispensable for the pollination and quality of the plant in
whatever way. Many of the medically and aromatic plants that grow spontaneously from nature are
valuable nectar resources for beekeeping. According to the flowering periods of these nectar sources,
migrating beekeeping activities are also carried out in our country. Thus, both the beekeepers reach
good honey resources and the continuity of medical and aromatic plant resources in our country is
ensured. This study is intended to increase the knowledge of beekeepers in our country about medical
aromatic plants and contribute to the emphasis of bee and apiculture activities on the medical
aromatics and other plants.

KEYWORDS
Medical and aromatic plant production, polination, beekeeping

Corresponding Author: AL GNC, ali.goncu@adu.edu.tr


1
ADNAN MENDERES NVERSTES NE MYO

1420
Poster Session 11
Submission ID: 1377

SCAVENGING ACTIVITY OF REACTIVE OXYGEN SPECIES AND


DETERMINATION ANTIOXIDANT PROPERTIES OF SIDERITIS
MONTANA

1 1 1
EMRE KO , SELVI CNGZ , FERDA CANDAN

ABSTRACT
Medicinal plants contain chemical component which show powerful antioxidant activity and
they can be used as a safe source in industrial foods, crops and products. Recently, there has been
reproducing interest in biochemistry of plants because of they can protect many diseases and health
problems such as aging, heart diseases and chronic diseases in human body. The Lamiaceae is a plant
family within that some species of them have potential therapeutic activity. The genus Sideritis which
is a member of the Lamiaceae family, commonly known as mountain tea in Turkey. These plants are
widely used to prepare herbal drug and traditional teas. In Turkey, the genus Sideritis contains 46
species, 31 of which are endemic .Plant materials of S.montana species were obtained from Yarhisar,
Hafik, Sivas-Turkey in 2011. The aim of this study was to investigate in vitro antioxidant activities of
methanolic extracts of Sideritis montana. Also, our investigations included total phenolic content, total
flavonoid content, total antioxidant capacity, scavenging of hydroxyl radicals, scavenging of DPPH
radicals and scavenging non-free radical species such as hydrogen peroxide. The extract of S.montana
showed that equivalent of phenolic content (157.29 3.71 mg GAE/g DW), flavonoid content (403.99
0.92 mg QUE/g DW) and total antioxidant capacities as (154.47 4.12 mM -tocopherol acetate /
gram dry weight). IC50 is the amount of extract supplying 50% inhibition of DPPH, hydrogen
peroxide and hydroxyl radicals. The IC50 values are presented in Table 1. Table 1 Scavenging of ROS
and DPPH for the methanolic extracts of Sideritis montana. Sample Hydrogen peroxide H2O2 IC50
(mg L-1) Hydroxyl radicals (OH) IC50 (mg L-1) DPPH radicals IC50 (mg L-1) Sideritis montana
25.05 1.46 124.75 3.04 58.48 1.30 Values are mean of triplicates SD The result of this study
indicate that methanolic extracts of S.montana can be useful as an easily accessible source of natural
antioxidants. By reason of in order to use these valuable Sideritis species in pharmaceutical products
and food, their cultivation and conservation are of great importance.

KEYWORDS
Sideritis montana, Antioxidant, DPPH, Reactive Oxygen Species.

Corresponding Author: EMRE KO, emrekoc@cumhuriyet.edu.tr


1
CUMHURYET NVERSTES

1421
Poster Session 11
Submission ID: 1380

ASSESSMENT OF THE ANTIOXIDANT ACTIVITY OF NERIUM


OLEANDER

1 2 1
SEVCAN GUL AKGUN , EVREN ALGIN YAPAR , SEMRA SARDAS

D
ABSTRACT
Burn injury is a complex traumatic event with various local and systemic effects like shock,

TE
anemia, and renal failure and electrolyte imbalances. The American Burn Association indicated that
more than 205,000 patients were admitted to burn centers across the United States, with 6,000 burn-
related deaths. Current data shows that burn injury is a big concern also in Turkey with approximately
70,000 patients applying to the burn centers every year. Several studies have demonstrated that
reactive oxygen species (ROS) triggered lipid peroxidation is one of the most harmful events
EN
occurring secondary to burn injuries, resulting in decreased membrane fluidity, altered membrane
properties and disrupted membrane-bound proteins. Moreover, recent evidences suggest that over
production of ROS can cause the formation of DNA single- and double-strand breaks, adducts, and
cross-links to other molecules. Although healing occurs by itself, the scientists and pharmaceutical
ES
companies focus on a variety of traditional herbal remedies to accelerate the process. There are a
number of natural ingredients identified via the clinical and laboratory studies with the support of
ancient knowledge for their common beneficial use in skin care and consumers often express a
preference for skin products with organic and natural ingredients. Nerium oleander (Apocynacea) is
one of those plants that has been the subject of research for centuries as a medical herb, and famed for
PR

its therapeutic efficiency in different diseases globally. The treatment of dermatological disorders such
as dermatitis, eczema, psoriasis, boils, herpes, ringworm, scabies, and warts is among its traditional
uses which can be attributed to its antibacterial, antiviral, anti-inflammatory and antioxidant activities.
The aim of this study was to evaluate the beneficial skin care effect of Aloe vera-based extract of
Nerium oleander (NAE-8) with regards to its antioxidant properties. For this purpose, in this study
the rats were randomly divided into 4 groups: i) control, ii) burn alone, iii) burn with topical NAE-8
T

treatment and iv) burn with topical 1% Silver sulphadiazine treatment. Antioxidant property of NAE-
8 has been examined by determination of glutathione (GSH) levels and myeloperoxidase (MPO)
O

activity. The results of the present study revealed that burn injury caused a significant (p<0.001)
depletion in the GSH stores while NAE-8 and SSD treatments reversed the GSH levels back to the
N

control (p < 0.001 and p < 0.05, respectively). Besides, there was a significant increase in MPO
activity in the burn group (3.940.71; p<0.001) as compared to control group (0.770.12) and this
elevation was abolished by both NAE-8 and SSD treatments (1.580.08 and 2.810.61,
respectively). The results showed that NAE-8 could be an option in burn wound treatment which
needs to be confirmed with further studies.

KEYWORDS

Corresponding Author: SEVCAN GUL AKGUN, sevcan.akgun@marmara.edu.tr


1
MARMARA UNVERSTY, FACULTY OF PHARMACY
2
SALIK BAKANLII, TRKYE LA VE TIBB CHAZ KURUMU

1422
Nerium oleander, Antioxidant activity, MPO, GSH

1423
Poster Session 11
Submission ID: 1381

TURKEY IN THE RAPID ALERT SYSTEM FOR FOOD AND FEED


NOTIFICATIONS IN THE PERIOD OF 2006-2016: HERBS AND SPICES
REVIEW

1 2
SAMI GKHAN ZKAL , SENEM TFEK

ABSTRACT
Turkey in the Rapid Alert System for Food and Feed notifications in the period of 2006-2016:
herbs and spices review Sami Gkhan ZKAL1, Senem TFEK2* 1 Pamukkale University,
Faculty of Engineering, Department of Food Engineering, Denizli, Turkey, 2 Pamukkale University,
Vocational School of Acpayam, Department of Food Processing, *Presenting Author: S. TFEK
(stufekci@pau.edu.tr) Herbs and spices were used in traditional medicine, food preparation and
preservation for thousands of years. Herbs and spices are vulnerable to biological, chemical and
physical contamination and hazards during supply chain. The Rapid Alert System for Food and Feed
(RASFF) database which is an effective tool to exchange information about measures taken
responding to serious risks detected in relation to food and feed can be also a good source for
investigating supply chain contaminations and hazards of herbs and spices. In parallel with this
purpose RASFF database was investigated for herbs and spices categorized notifications originating
from Turkey cover period from 2006 to 2016. RASFF notifications were divided into four groups as;
alerts, information, border rejection, and news. A total of 59 notifications (alert 21, information 19,
border rejection - 19) were reported for Turkey against a worldwide 1685 notifications. Oregano was
the most border rejected herb. The number of notifications showed a descending trend
chronologically. Germany, Italy and Netherlands were countries most notified about herbs and spices
via Turkey. Listed 26 hazard categories were analyzed for herbs and spices and the major hazards
categories were composition (45.76 %), pathogenic micro-organisms (35.59 %) and mycotoxins
(11.86 %). The relation between hazard category and product was also examined, and mycotoxin
hazards mainly seen in chilli pepper, paprika and red pepper. Salmonella spp. was only frequently
reported pathogenic micro-organism in RASFF for Turkey with 20 notifications. In all categories the
most notified products were oregano, chilli pepper, red pepper and cumin with 13.56 %, 11.86 %,
10.17 %, 10.17 % respectively. Keywords: The Rapid Alert System for Food and Feed (RASFF),
herbs, spices, notifications, Turkey

KEYWORDS
The Rapid Alert System for Food and Feed (RASFF), herbs, spices, notifications, Turkey

Corresponding Author: SENEM TFEK, stufekci@pau.edu.tr


1
PAMUKKALE UNVERSTY, FACULTY OF ENGNEERNG, DEPARTMENT OF FOOD ENGNEERNG
2
PAMUKKALE UNVERSTY, VOCATONAL SCHOOL OF ACIPAYAM, DEPARTMENT OF FOOD PROCESSNG

1424
Poster Session 11
Submission ID: 1382

PLANTS USED AS COFFEE IN EASTERN MEDITERRANEAN


REGION

1 2 3 2
SERTAN EVK , HSNIYE ZALP , MACIDE BURCU TMUR , RIZA BNZET

ABSTRACT
Coffee is one of the most important agricultural plantation crop. All cultivated species
originate from Africa and belong to the genus Coffea. The genus Coffea comprises approximately 100
species and only two species (Coffea arabica L. and Coffea canephora Pierre ex A.Froehner) are
commercially cultivated. It is grown in about 10.2 million hectares land spanning over 80 countries in
the tropical and subtropical regions of the world especially in Africa, Asia, and Latin America (Mishra
and Slater, 2012). Coffee is the most important commodity in international agricultural trade,
generating over 90 billion dollars each year and involving about 500 million people in its
management, from cultivation to final product for consumption (Dias et al. 2007; Cavatte et al. 2012,
Praxedes et al. 2006). Coffee is the second most consumed drink in the world after water and it is
known that approximately five hundred billion cups of coffee are consumed annually (Butt et al.
2011). In this study, 15 different plant species belonging to 9 families used as coffee in the Eastern
Mediterranean Region were discussed. This species are respectively: Prunus dulcis (mill.) D.A Webb,
Nigella sativa L. Cichorium inthybus L. Gundelia tournefortii L. Onopordum illyricum L. Pistacia
terebinthus L. Pistacia atlantica L. Cicer arietinum L., Abelmos chusesculentus (L.) Moench Echinops
ritro L. Juniperus communis L. Ceratonia siliqua L. Elettaria cardamomum (L.)Maton, Phoenix
dactylifera L. Taraxacum officinale G. In which parts of these species are discussed, information is
given on how to make and prepare them. Obtained information is usually obtained by interviewing
elderly people and local counselors (The turkish name is Aktar) face to face. Family, genus, species,
Turkish names and coffee preparation are given for each taxon.

KEYWORDS
Coffee, East Mediterranean, Etnobotany, East Mediterranean, Turkey

Corresponding Author: SERTAN EVK, srtncvk@gmail.com


1
MERSN UNVERSTY, VOCATONAL SCHOOL OF MUT, MERSN
2
MERSN UNVERSTY, SCENCES AND ARTS FACULTES, DEPARTMENT OF BOLOGY, 33343, MERSN
3
MERSN NVERSTES, DEPARTMENT OF BOLOGY, FACULTY OF ARTS AND SCENCE, MERSN
UNVERSTY, 33343, MERSN, TURKEY

1425
Poster Session 11
Submission ID: 1383

DETERMINATION OF SLIMMING HERBAL TEA CONSUMPTION OF


COLLEGE STUDENTS

1 1 1 1 1 1
ELMAS ERSZ , EZGI TOPTA BIYIKLI , ALI EMRAH BIYIKLI , KBRA TOP , SIBEL YANAR , HATICE NUR TURAN ,
1 1
HILAL DIKILITA , OKAN GLER

ABSTRACT
INTRODUCTION Obesity is one of the increasing health issue of nowadays and due to this,
attention to the herbal products increased. Persons who want to lose weight, have a false belief that
these products could lose weight easily and swiftly. Especially the cost and availability of the herbal
teas, let people to prefer them. Herbal teas could help to lose weight due to herbal teas caffeine content
and effects on catechin thermogenesis. In addition to this positive influence, herbal teas have laxative,
diuretic and sweating properties. OBJECTIVE This surveys aim is to determine the slimming herbal
tea consumption of college students. METHOD The survey is made between February 2017 and
March 2017 on randomly selected students at Seluk University. 338 women and 35 male students are
including for this study. Specially prepared survey is used for this study. In the survey, general
characteristics, their herbal tea consumption for slimming in the last year and their choice reasons
were asked. Besides the participants anthropometric measurements have measured. To evaluate the
data, SPSS 22.0 program is used. Chi square test is applied for the relationship between the parameters
and significance level is set as p<0.05. RESULTS The average age of the participant students is
20.21.5. Body mass index of the students are found as low weight, normal weight and overweight,
11.8%, 70.0% and 18.2% respectively. 15% of the participants use herbal teas to lose weight.
Womens use of slimming herbal tea rate is 15.7%. On the other hand, males use of slimming herbal
tea is 8.6%. 12.9% of the participants prefer green tea, 6.2% prefer form tea for losing weight. People
with normal weight who drink green tea is 66.7% and the rest who drink green tea are over weighted
people. There is a meaningful relationship (p=0.000) between body mass index and green tea
consumption. 56.5% of the students who drink form tea is at normal weight and the rest is over
weighted. There is a meaningful relationship (p=0.002) between body mass index and form tea
consumption. CONCLUSION Obesity is a significant health issue and it is increasing in prevalence.
Fast food, life style changes and sedentary life style causes obesity to increase among the college
students. Importance for the body image at the teenagers, increases tendency towards slimming tea
consumption. In this study, it is remarkable that, the rate of the students who use slimming herbal tea
is too high whether the body mass index of most of the students are at normal levels. Over use of
slimming herbal tea could show laxative and diuretic effects on health. Students should be informed
about healthy nutrition and possible risks of the methods applied during weight loss diet.

KEYWORDS
obesity, herbal tea, green tea

Corresponding Author: ELMAS ERSZ, elmasonde@gmail.com


1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEKOKULU BESLENME VE DYETETK
BLM

1426
Poster Session 11
Submission ID: 1384

IN VITRO ANTIOXIDANT ACTIVITIES OF METHANOLIC EXTRACT


OF ONOSMA SERICEUM

1 2 2
MERYEM ORAKCI , EMRE KO , FERDA CANDAN

ABSTRACT
Onosma species (Boraginaceae) largely grow in Turkey. Eighty five species involving forty
four endemic species has been represented by now. The genus has several usage in the traditional
medicines worldwide. The aim of this study is to investigate in vitro antioxidant activities of
methanolic extract of Onosma Sericeum. We examined the in vitro radical scavenging and antioxidant
capacity of Ebenus Laguroides by using different in vitro analytical methodologies such as 1,1-
diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, 2,20-azino-bis (3-ethylbenzthiazoline-6-
sulfonic acid) (ABTS) radical and N,N-dimetil-p- fenilendiamin (DMPD) radical scavenging activity,
total antioxidant activity determination by ferric thiocyanate, total reducing ability determination using
by Fe3+Fe2+ transformation method, hydrogen peroxide scavenging and ferrous ions (Fe2+)
chelating activities. Also, The synthetic antioxidant butylated hydroxytoluene (BHT) and natural
antioxidant such as curcumin and ascorbic acid were used as positive controls. The Table 1. represents
the antioxidant potential of methanolic extract of Ebenus Laguroides by ferric thiocyanate method,
2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, 1,1-diphenyl-
2-picryl-hydrazyl (DPPH) free radical scavenging activity, N,N-dimethyl-p-phenylenediamine
(DMPD) radical scavenging activity. Table 1 Results of the antioxidant potential of methanolic extract
Onosma Sericeum by the different in vitro antioxidant assays Sample DPPH scavenging activity (g
mL-1) ABTS+ scavenging activity (%) DMPD+ scavenging activity (%) Metal chelating Activity
(%) Onosma Sericeum 54.746.25 61.650.08 54.842.16 19.901.30 Values are mean of triplicates
SD The results of the study have shown that the methanol extract of Onosma Sericeum is potentially
a good source of free radical scavenging compounds. Acknowledgements: This research was part of
the project number F-347 supported by the Research Council of Cumhuriyet University in Sivas /
Turkey.

KEYWORDS
Onosma Sericeum; antioxidant activity, radical scavenging, metal chelating, reducing power,

Corresponding Author: EMRE KO, emrekoc@cumhuriyet.edu.tr


1
2
CUMHURYET NVERSTES

1427
Poster Session 11
Submission ID: 1385

SOME CHEMICAL PROPERTIES OF RHEUM RIBES L. (UKUN)


AND BENEFICAL EFFECTS ON HEALTH

1 1
MEHMET MURAT CEYLAN , MENEKE BULUT

ABSTRACT
Rheum ribes L. is a kind of edible wild that belongs to the Polygonaceae family, and also it is
one of the perennial plants. R. ribes is locally known as ukun, gn, ravent, revas and it is grown
especially in the east part of Turkey, Iran, Lebanon and Iraq. R. Ribes is the only rhubarb species
grown between 1800 m. and 2800 m. altitute rocky countryside of Turkey. According to some studies
Rheum ribes is the source of one of the most important crude drugs in west asiatic regions. These
plants contain vitamins A, B, C in abundance. Rhubarb root (Rhizoma Rhei ribi) is used traditionally
to treat diabetes, hemorrhoids, ulcers and diarrhea. The plant is also used as a digestive and appetizer
in Bitlis, Turkey. In some countries such as Iran, Rheum ribes has been used as sedative and mood
enhancer. These traditional edible wilds were genarally used in medicine for the treatment of anemia,
anorexia, weakness, anxiety, depression and diabetes. On the other hand; there are some studies about
mineral content of R. ribes. According to these studies; R. ribes a rich content of ferrous and phosphor.
In this study, some chemicals properties of ukun and benefical effects on health have been
reviewed.

KEYWORDS
Ukun, Rheum ribes, medicine treatment

Corresponding Author: MENEKE BULUT, gidabenefse@gmail.com


1
IDIR NVERSTES

1428
Poster Session 11
Submission ID: 1386

SPECTROPHOTOMETRIC DETERMINATION OF REBAUDIOSIDE A


IN AQUEOUS SOLUTIONS

1 1 1 1 1
SEMIH LATIF PEK , ALI EMRAH ETIN , NURTEN KURT CENGIZ , HAIM KELEBEK , OSMAN KOLA , MEHMET
2
SERTA ZER

ABSTRACT
Steviol glycosides are natural compounds found in the leaves of Stevia Rebaudiana Bertoni.
Stevioside and Rebaudioside A are the major glycosides among the several steviol glycosides
identified in the Stevia. They have received special interest in both scientific researches and industry
due to their 150 to 570 fold sweetening power relative to sucrose. Steviol glycoside composition of
Stevia leaves, solutions obtained during extraction and purification processes as well as the purity of
the final products is determined by High Pressure Liquid Chromatography (HPLC). HPLC enables the
separation and quantification of each steviol glycoside. Since the studies on the determination of
solubility, adsorption-desorption, dissolution and crystalization kinetics usually employ aqueous
solutions containing only Rebaudioside A as a solute, the use of a fast, cheap and easy-to-perform
method will be appropriate for the determination of Rebaudioside A concentration. The goal of this
study was therefore to evaluate spectrophotometry for the determination of Rebaudioside A
concentration in aqueous solutions. In this study, two different sets of Rebaudioside A standard
solutions (10 90 mg/L and 100 200 mg/L) were prepared and absorbances were measured at 210
nm for the construction of calibration curves. Five solutions with concentrations of 15, 25, 75, 130 and
170 mg/L were then used as control samples to evaluate percent recoveries and percent relative errors
in the determination of Rebaudioside A. HPLC method was also selected as a standard method for
comparison. Standard solutions and control samples were analyzed by HPLC equipped (Agilent 1260)
with C18 column and diode array detector using water-acetonitrile mixture (70:30, v/v) as a mobile
phase at 210 nm with an injection volume of 5 L. Simple spectrophotometric measurements of
absorbances of standard solutions gave linear calibration curves with correlation coefficients (R2) of
0.995 and 0.998 for 10-90 mg/L and 100-200 mg/L concentration ranges, respectively. HPLC method
however yielded linear calibration coefficient of 0.999 for both sets of standard solutions. Percent
relative errors calculated in the determination of Rebaudioside A concentration in control samples by
spectrophotometric method were 46, 23, 8, 6 and 3 % while HPLC method gave percent relative errors
as -26, -12, -10, -9 and -5 % for 15, 25, 75, 130 and 175 mg/L concentrations, respectively. The results
of this study showed that the percent relative errors associated with spectrophotometric determination
were high for lower concentrations. HPLC yielded lower percent relative errors for low concentrations
when compared to spectrophotometric method. The percent relative errors associated with
spectrophotometric determination of Rebaudioside A concentration over 75 mg/L were lower than
those found in HPLC based method. As a result, simple spectrophotometric measurement can be used
to determine Rebaudioside A in the aqueous solutions with concentrations over 75 mg/L.

Corresponding Author: SEMH LATF PEK, slipek@adanabtu.edu.tr


1
ADANA BLM VE TEKNOLOJ NVERSTES
2
UKUROVA NVERSTES

1429
Acknowledgement: This work was supported by Adana Science and Technology University Scientific
Research Coordination Unit. Project Number: MUHDBF.GIDA.2015-9.

KEYWORDS
Stevia, Steviol glycosides, Rebaudioside A, Spectrophotometry, HPLC

1430
Poster Session 11
Submission ID: 1389

OUR NATURAL ELIXIR: GANODERMA LUCIDUM (CURTIS) P.


KARST.

1 2
HAKAN ALLI , BEKIR L

ABSTRACT
Introduction: Since ancient times, only emperors and their children in the Far East have been
able to drink the juice of Ganoderma lucidum (Reishi). When someone finds this mushroom under a
tree, he had to report it to the local court and hand it over to the authorities. It is known that the houses
in the region where the mushroom is found are searched and the people possessing this mushroom
were severely punished. The mushroom, which has been used in Far East traditional medicine for
about 2000 years is also known as the "Queen of Fungi" and it was first cultured by the Japanese
researcher Shigeaki Mori. After that, it has attracted the attention of many people from all parts of the
world. Material and Methods: Specimens of macrofungi were collected from Mula province during
the routine field trips between 2015 and 2016. In the field, ecological and macroscopic features were
recorded and photographed. After the field studies, the specimens were brought to the laboratory and
identified morphologically using reference books. Results: Ganoderma lucidum, which has been used
in some health problems since ancient times is very difficult to be spotted and identified in nature and
it is significant for our country that this mushroom is found naturally in different locations of our
country. Discussion: It is very risky to collect and use this mushroom that has the medicinal value by
the people other than the mushroom experts. Moreover, it will not provide much benefit to use the by-
products of this fungus, which are claimed by some people in the market to have some active
ingredients. Therefore, it is best to consume the mushroom itself and not the side products. It is a very
important step that Reishi mushroom has recently been started to being cultured in our country.

KEYWORDS
Ganoderma lucidum, Medicinal Mushroom, Turkey

Corresponding Author: HAKAN ALLI, hakanalli@gmail.com


1
MULA SITKI KOMAN NVERSTES FEN FAKLTES BYOLOJ BLM

1431
Poster Session 11
Submission ID: 1390

USAGE AREAS OF SOME SPICE PLANTS AND EVALUATION IN


TERMS OF COUNTRY ECONOMY IN TURKEY

1 2 1
AYBKE KAYA , DILEK BOSTAN BUDAK , ERDAL DAISTAN

ABSTRACT
Turkey has different climate characteristics. It is a country rich in biological diversity. Turkey
is the motherland of many plant species in terms of its position in the world. Medical and aromatic
plants are also naturally grown in Turkey. Especially spice plants have been used all over the world for
centuries both in local kitchen and in the health field. Health problems have increased in recent years.
These problems are solved by using fewer chemicals. The demand for these plants has increased
factors such as increasing health problems and solving these problems by using fewer chemicals,
herbal teas used to prevent obesity, development of cosmetic industry and preference of different
aroma substances in recent years. Many spice plants grow in Turkey. The most produced plants are red
pepper, anise, thyme, cilantro and coriander. The planting area and production of these plants have
shown fluctuations and have increased in recent years. Turkey was realized in 2016 approximately 12
500 ha area 228 531 tonnes red pepper, 9,491 tonnes of anise from 14 000 ha area, 18 586 tonnes of
cumin from 27 000 ha area, 14 724 tonnes of thyme from 12 000, 2527 tonnes of black sesame from
2350 ha area, 2464 tonnes of fennel from 1750 ha area, 42 tonnes of coriander from 50 ha area and
1883 tonnes of broom from 1400 ha area. Production is mostly in rural areas. There are more female
labor force in this area. Employment of women in this area increases the level of income, it also
positively affects the level of regional development. In addition, with the effect of the increasing
population, the demand for these plants is contributing to the demand, it is also developing foreign
trade and contributing significantly to the economy of the country. Spice plants have an important
place in Turkey. The aim of this study to determine the contribution of some spice plants to the
economy of the country.

KEYWORDS
Spice plants, Economy, Female labor force, Foreign Trade, Turkey

Corresponding Author: AYBKE KAYA, aybukekaya@mku.edu.tr


1
MUSTAFA KEMAL NVERSTES
2
UKUROVA NVERSTES

1432
Poster Session 11
Submission ID: 1391

MEDICINAL PROPERTIES OF CORIANDRUM SATIVUM

1 1 1
H. EDA USTUNTAS , SENAY OZGEN , ZELIHA SELAMOGLU

ABSTRACT
Cilantro (Coriandrum sativum L.) is also known as coriander or Chinese parsley. It is an
annual plant of family Apiaceae. It is known from the ancient times as a spice and cultivation of
cilantro is now reported from many countries, e.g. Argentina, Brazil, Burma, China, Egypt, Ethiopia,
France, Great Britain, India, Italy, Libya, Mexico, Morocco, the Netherlands, Paraguay, Peru, Poland,
Rumania, Somalia, Spain, USA, USSR, and Yugoslavia. The stem is smooth, feeble and light green in
color. The leaves are compound, thin, alternate and easily breakable and having spherical fruits with
the diameter of one centimeter. The fresh leaves of coriander are nutrient rich which include a variety
of minerals along with proteins and carbohydrates. Cilantros all plant parts are edible but usually the
fresh leaves and the dried seeds are more preferred for cooking. Coriander juice is beneficial as it
contains vitamin A, B1, B2, C and Fe. Besides its nutritional value, the plant has medicinal
applications too. In Ethiopia, fruits of coriander are used against stomachache. For this purpose, the
fruit extract is taken orally to cure this ailment. The leaves are chewed to control colic and
stomachache. It is also used as traditional medicine to heal intestinal diseases, inflammation and joint
pain and rheumatism. In addition to these, coriander essential oil is used in perfumery industry.

KEYWORDS
Cilantro, Medicinal plant, Coriander, Chinese parsley.

Corresponding Author: H. EDA USTUNTAS, edaustuntas88@gmail.com


1
MER HALSDEMR UNVERSTY

1433
Poster Session 11
Submission ID: 1394

OPTIMIZATION OF DRYING MEDICINAL AND AROMATIC PLANTS


BY RESPONSE SURFACE METHODOLOGY

1 2
SAMI GKHAN ZKAL , SENEM TFEK

ABSTRACT
Optimization of Drying Medicinal and Aromatic Plants by Response Surface Methodology
Sami Gkhan ZKAL1, Senem TFEK2* 1 Pamukkale University, Faculty of Engineering,
Department of Food Engineering, Denizli, Turkey, 2 Pamukkale University, Vocational School of
Acpayam, Department of Food Processing, *Presenting Author: S. TFEK (stufekci@pau.edu.tr)
Medicinal and aromatic plants are consumed as fresh but they have short shelf life due to
environmental conditions limiting the long term consumption. Drying is one of the oldest used
methods for preservation of medicinal and aromatic plants. Although drying process enables long shelf
life by removal of water from structure, it has several advantages such as chemical, physical and
nutritional losses, undesirable flavor, and color changes. Using a pre-drying treatment or combining
different drying methods are some way of producing dried products at good quality with minimum
losses. In order to obtain better quality dried products there is a need to understand drying process by
determining drying characteristics of plants at different drying conditions. Optimization of drying
process is essential for medicinal and aromatic plants. For this purpose optimization by response
surface methodology can be used. Response surface methodology (RSM) is a useful method for
evaluating the relationship between the responses (drying characteristics, quality parameters) and
independent variables (drying conditions). RSM uses data evaluated from experimental designs and
represents surfaces which describe the independent variables effects on responses. Interrelationships
among the drying conditions and combined effect of all process conditions on quality parameters can
be provided statistically and graphically by this method. The aim of this study was to investigate the
optimum drying conditions for medicinal and aromatic plants such as; acerola, blackcurrant,
blueberry, garlic, bitter gourd, ginger, green peppers, jackfruit, mango, mushroom, olive leaves, onion,
pandan leaves, parsley, pink guave, spirulina, turmeric. IN this review drying and pre-treatment
methods, conditions (air temperature, air velocity,etc.), experimental designs and effects of them on
selected responses as quality parameters for these products will be explained briefly.

KEYWORDS
Response Surface Methodology (RSM), optimization, drying, medicinal and aromatic plants

Corresponding Author: SENEM TFEK, stufekci@pau.edu.tr


1
PAMUKKALE UNVERSTY, FACULTY OF ENGNEERNG, DEPARTMENT OF FOOD ENGNEERNG
2
PAMUKKALE UNVERSTY, VOCATONAL SCHOOL OF ACIPAYAM, DEPARTMENT OF FOOD PROCESSNG

1434
Poster Session 11
Submission ID: 1395

IN VITRO ANTI-INFLAMMATORY ACTIVITY OF ACHILLEA


SIPIKORENSIS HAUSSKN. & BORNM.

1 1
SELVI CNGZ , FERDA CANDAN

ABSTRACT
Inflammation is an immediate response to many injuries produced by pathogens, noxious
stimuli such as chemicals, or physical injury. Inflammation can cause various physical dysfunctions.
Inflammatory disorders are treated using conventional anti-inflammatory drugs such as steroidal anti-
inflammatory drugs and nonsteroidal anti-inflammatory drugs (NSAIDs). However, their prolonged
use may produce adverse effects. Thus, it is important to develop new anti-inflammatory agents with
fewer adverse effects. Natural products can be a source of active metabolites that can serve as an
alternate approach to anti-inflammatory drugs. The search for natural products with anti-inflammatory
activity has increased markedly in recent years. Achillea species have been used for their anti-
inflammatory, antidiabetic, analgesic, hemostatic, spasmolytic, digestive and cholagogue effects in
Turkish folk medicine. Dry materials of Achillea sipikorensis from Hausskn. & Bornm. were obtained
Grn, Sivas in June 2012. This study was aimed to evaluate the anti inflammatory activity of both
methanolic and aqueous extract of the flowers of Achillea sipikorensis Hausskn. and Bornm by human
red blood cell membrane stabilization method and protein denaturation, using different extract
concentrations. Both results are compared with standard diclofenac. The result obtained from this
study suggests that extracts of Achillea sipikorensis has a natural source for anti-inflammatory
activity. However, further studies must be conducted in order to clarify which constituent(s) of the
extracts is responsible for these activities. Acknowledgements: This research was part of the project
number F-501 supported by the Research Council of Cumhuriyet University in Sivas / Turkey.

KEYWORDS
Achillea sipikorensis Hausskn. and Bornm., anti-inflammatory, HRBC membrane
stabilization; albumin denaturation; diclofenac.

Corresponding Author: SELV CNGZ, selvi.cingoz@gmail.com


1
CUMHURYET NVERSTES

1435
Poster Session 11
Submission ID: 1396

SOME MEDICINAL AND AROMATIC PLANTS FROM ORDU


VICINITY

1 1 1
ZNUR ERGEN AKN , TUBA ZBUCAK , GLCAN ENEL

ABSTRACT
Some Medicinal and Aromatic Plants from Ordu Vicinity znur ERGEN AKN1, Tuba
ZBUCAK2, Glcan ENEL3 1 2Ordu University, Faculty of Science and Art, Department of
Molecular Biology and Genetics, Ordu,Turkey. 3 Ondokuz Mays University, Faculty of Science and
Art, Department of Biology, Samsun, Turkey oakcin@gmail.com Many local wild plants have been
used as traditional medicinal plants in Anatolia. Wild medicinal plants are very widespread in Black
Sea Region of Turkey and people has been used wild plants as medicinal purposes. In this study, some
wild medicinal plants which used in the treatment of diseases in Ordu were investigated and
introduced. Specimens were collected from different locaties in Ordu. Their morphological features,
parts used as medicinal plants, methods of using, scientific name and Turkish name were determined.
There are about 80 wild medicinal plants in Ordu vicinity. In this study, common 15 wild medicinal
and aromatic plants were investigated. These plant are Helichrysum sp., Hypericum orientale L.,
Hypericum bithynicum Boiss., Helleborus orientalis Lam.,Tussilago farfara L., Ornithogalum
sigmoideum Freyn et. Sint., Cyclamen coum ssp. coum, Arum italicum Mill., Urtica dioica L., Malva
neglecta Wallr., Laurocerasus officinalis Roemer, Tilia rubra DC., Cichorium intybus L.,
Rhododendron luteum Sweet. and Chelidonium majus L. Generally leaf, stem and flowers of plant are
used as medicinal purposes. Flowers and leaves of Hypericum orientale, Tussulago farfara and
Rhododendron luteum are used as therapeutic. Arum italicum has expectorant and cough suppressant
properties. Aboveground parts of Helichrysum are used diuretic. Chelidonium majus is used as
diuretic and expectorant. Also the plants sap is used against warts and corns. Cylamen is effective in
treating arthritis and joint pain. Also used in the treatment of boils and burns. Ornithgalum
sigmoideum is good for pimples and boils. This plant has diuretic and emetic effect. Urtica dioica is
effective against rheumatic pain and eczema. Keywords: Medicinal plant, aromatic plant, traditional
medicine, Ordu, Turkey.

KEYWORDS
Medicinal plant, aromatic plant, traditional medicine, Ordu, Turkey.

Corresponding Author: ZNUR ERGEN AKN, oakcin@gmail.com


1
ORDU NVERSTES

1436
Poster Session 11
Submission ID: 1400

ANTIDOTIC POTENCY OF BITTER GOURD (MOMORDICA


CHARANTIA L)

1 1 1
MUHAMMAD YASIR NAEEM , SENAY OZGEN , ZELIHA SELAMOGLU

ABSTRACT
Both in developed and developing countries the people have been using herbal medicine since
aging to cure several life-threatening diseases such as cancer, diabetes, obesity and heart disease. In
recent years an herbaceous vegetable called Bitter gourd (Momordica charantia L) with tropical and
subtropical distribution has been identified to be used effectively against these diseases. It is one of the
most valuable marketable vegetable in south-eastern countries of the Asia and spread out all over the
world due to its high nutritive and medicinal value. It is commonly called Aci kabak in Turkey, with
various local names like balsam pear, African cucumber and bitter melon around the world. It is ever
first domesticated in eastern India and carried to china near 14th century, while due to slave tradition
in ancient world it spread out to some parts of the world for medicinal and food purpose. Bitter gourd
is available in the natural of basket which offers excellent medicinal merits. The bitter gourd is
specially used to control diabetes as well as several fungal diseases like scabies, ring worm, psoriasis
and also blood disorder like blood boils. Leaves and roots of bitter gourd have different compounds
which are using in traditional medicines for respiratory diseases. The leaves tips of bitter gourd are the
good source of vitamin A. Fresh fruits of bitter gourd have compounds like Charantin, Peptides,
glycosides, triterpines and alkaloids actively affects the blood and urine sugar level. Juices from the
fresh leaves of bitter gourd also highly effective in diseases like diarrhea and cholera. Bitter gourd
contains proteins like MAP30 (exposed by American scientists in 1996) having great effect against
diseases like AIDS, tumor and other viruses like herpes simplex virus 1 (HSV-1) inhibiting its
reproduction as well as reducing its ability to skin irritation. Bitter gourd has high contents of fiber in
their fruit help in many stomach disorders by stimulating gastric juice. A-electrostatic acid and 15, 16-
dihydroxy-a-eleostearic acid are the two compounds found in seeds and fruits of bitter gourd
respectively, contribute great effect to health by preventing cancerous cell, help in production of red
blood cells, leukemia disorder and also control blood pressure. Alpha and beta-momorchardin protein
present in bitter gourd have constraining result on human immune deficiency virus (HIV) infection. It
is inviting scientists across the world due to its high medicinal value in treatment of various diseases
especially in diabetes mellitus and respiratory diseases.

KEYWORDS
diabetes mellitus, Charantin, Peptides and MAP30

Corresponding Author: MUHAMMAD YASR NAEEM, yasr.naeem@yahoo.co.uk


1
MER HALSDEMR UNVERSTY

1437
Poster Session 11
Submission ID: 1401

IN VITRO ANTI-DIABETIC ACTIVITY OF AQUEOUS EXTRACT OF


THE MEDICINAL PLANT BERBERIS CRATAEGINA DC. FRUITS

1 1 1
FERDA CANDAN , FERHAN CANDAN , EROL DNMEZ

ABSTRACT
Recently, some medicinal plants have been reported to be useful in diabetes worldwide and
have been used empirically as antidiabetic remedies. Despite the presence of known antidiabetic
medicine in the pharmaceutical market, diabetes and the related complications continued to be a major
medical problem. Antihyperglycemic effects of medicinal plants are attributed to their ability to
restore the function of pancreatic tissues by causing an increase in insulin output or inhibit the
intestinal absorption of glucose or to the facilitation of metabolites in insulin dependent processes.
More than 400 plant species having hypoglycemic activity have been available in literatre. However,
searching for new antidiabetic drugs from natural plants is still attractive because they contain
substances which demonstrate alternative and safe effects on diabetes mellitus. The present
investigation evaluated aqueous extract of Berberis crataegina DC was subjected to inhibitory effect of
- -Glucosidase inhibition assay using specific standard in vitro
procedure. Acarbose was utilized as the positive control. The extract produced higher reduction of -
glucosidase activity than -amylase. The findings indicate Berberis crataegina DC possess
hypoglycemic effect and hence it can be utilized as an adjunct in the management of diabetes mellitus.

KEYWORDS
- amylase; -glucosidase; In vitro antidiabetic; Berberis crataegina DC

Corresponding Author: FERDA CANDAN, candan@cumhuriyet.edu.tr


1
CUMHURYET NVERSTES

1438
Poster Session 11
Submission ID: 1402

THE PROTECTIVE EFFECT OF GERANIOL AGAINST REMOTE


TISSUE INJURY INDUCED BY SHORT TERM RENAL ISCHEMIA /
REPERFUSION IN RATS

1 1 1 2 1 1
IIL TAN YILMAZ , MEDIHA CANBEK , HAKAN ENTRK , FATMA YILDIZ , SEREN DANI , SENANUR CAN

ABSTRACT
In our study, the possible protective effects of geraniol, which is known to be an atioxidant,
were investigated against liver injury induced by experimentally short-term renal ischemia/reperfusion
(I/R) injury in rats. In the study, three to four month old, Wistar-albino type 28 rats were used (n=7).
Four groups were designed randomly that Group I (Sham Group), Group II (I/R+normal saline),
Group III (I/R+ 50 mg/kg geraniol), Group IV (I/R+ 100 mg/kg geraniol). Right nephrectomies were
performed under xylazine (10 mg/kg) and ketamine (70 mg/kg) anesthesia in all group rats. Then, 2 ml
physiological saline solution was injected to Group I and Group II; 50 mg/kg geraniol was injected to
Group III and 100 mg/kg geraniol was injected to Group IV intraperitoneally one hour before
ischemia. 45 minutes ischemia and 4 hours reperfsion were applied to all groups expect Group I. At
the end of the experiment, ALT, AST activites in the blood serum and the Catalase (CAT), Superoxide
dismutases (SOD) and Glutathione peroxidase (Gpx), enzime activities in liver tissue were measured.
Histological sections were stained using Hematoxylien & Eosine and investigated by light microscope.
According to the study results, when Group I and Group II s ALT and AST values were compared in
serum and CAT, SOD, Gpx in tissue samples, belonging to Group II s serum ALT and AST value and
SOD, Gpx activity increased and CAT activity decreased in liver tissue. While Group III and IV s
SOD and Gpx activities decreased, CAT activity increased comparad to Group II. Although
histopathologically, in Group II s the liver tissue was shown that intense vakuolization, advenced
necrozis, intense nuclear infiltrasyon and congestion, this findings was not found in Group IV.
Biochemical analyzes have supported by histological findings of our experimental study. The results
of this study have demonstrated that geraniol (100 mg/kg i.p.) prevents distant organ injury because of
renal I/R injury.

KEYWORDS
Ischemia/Reperfusion, Remote organ, Liver, Free Radical,Geraniol

Corresponding Author: IIL TAN YILMAZ, yilmazisiltan@gmail.com


1
ESKEHR OSMANGAZ NVERSTES
2
TOROS NVERSTES

1439
Poster Session 11
Submission ID: 1403

IN VITRO ANTI INFLAMMATORY ACTIVITY OF METHANOLIC


EXTRACT OF ACHILLEA TERETIFOLIA WILLD. BY HRBC
MEMBRANE STABILISATION

1 1
FERDA CANDAN , SELVI CNGZ

ABSTRACT
Plants from the genus Achillea are used as folk medicine for treating various diseases
including inflammatory and immune-related diseases. Numerous reports have suggested plant extracts
and their constituents as possible anti-inflammatory agents. Here, in vitro evidence of Achillea
teretifolia Willd. anti inflammatory activity and antioxidant properties is presented for the first time. In
this study we found out that the methanol extract of the leaves of Achillea teretifolia Willd. possess a
significant anti-inflammatory action comparing with diclofenac sodium as standard. Human red blood
cell (HRBC) membrane stabilization was taken as the screening procedure for obtaining the results. In
this particular method inhibition of membrane lysis was taken as the measure of anti-inflammatory
property. The haemoglobin content in the supernatant solutions was estimated using
spectrophotometer at 560 nm. The percentage haemolysis was calculated by assuming the haemolysis
produced in presence of distilled water as 100%. The maximum membrane stabilization of Achillea
teretifolia extracts was found to be 92.47 % at a dose of 1000 g/ml. Therefore, our present in vitro
studies on Achillea teretifolia extracts demonstrate the depression of inflammation.

KEYWORDS
Achillea teretifolia Willd., anti-inflammatory, HRBC membrane stabilization; diclofenac.

Corresponding Author: FERDA CANDAN, candan@cumhuriyet.edu.tr


1
CUMHURYET NVERSTES

1440
Poster Session 11
Submission ID: 1404

DETERMINATION OF THE THOUGHTS OF HEALTH SERVICE


VOCATIONAL SCHOOL STUDENTS ABOUT PLANT USE AND
HERBAL TREATMENT

1 1 1 1
GLSM YETI , TURGAY KOLA , PERIHAN GRBZ , ZEHRA DENIZ YAKINCI

ABSTRACT
Summary Objective: This cross-sectional study was conducted to determine the opinions of
Health Services Vocational School students about the use of plants and the use of herbal remedies.
Materials and Methods: The study was conducted with 342 students having education at nn
University Health Services Vocational School. A questionnaire which was prepared by the researchers
using the literature knowledge, consisting of questions about the socio-demographic characteristics of
students and their thoughts about herbal treatment was used in the collection of the data. Analyzes of
the obtained data were done by using SPSS 17.0 program, frequency and percentages were determined
and q-square test was performed. For the realization of the research, written approval from the
institution and the ethics committee, verbal approval from the students was obtained where the
research was conducted. Findings: 69.3% of the students who participated the research were female,
73.5% had medium economic status and the average age was 20.33 3.49. 7.6% of the students had a
chronic illness requiring medication. While 77.1% had a body mass index in the normal range, 7.0%
was below and 16.0% was abovethe normal range. Among the students; 70.2% of them had used
alternative treatment, 70.0% believed in alternative treatment, 52.4% had people using alternative
treatment around them, 45.1% had no idea about alternative treatment to be correct or not, 14.8% did
not find the alternative treatment correct, and 29.2% of those who did not find it correct expressed the
reason of this condition by the unconscious use of the patients. The second reason of this condition
was expressed by lack of training about herbal treatment (28,6%). 52.2% of the students stated that
they found both drugs and plants safe, 21.3% said they found plants more reliable than drugs, and
44.1% of the students stated that they used herbal treatment. The order of herbal treatment use is;
products that strengthen the body and the immune system (31.3%), skin care products (31.3%), hair
care products (22.3%), pain relief products (21.0%), products for burn treatment (9, 4), perfume
essences (7.6%) and slimming products (6.7%). It was found that 70.6% of the students using herbal
products used the products in need, 40.9% bought them from the herbalists, 61.6% recommended the
products they used to other people, 32.5% used herbal treatment to benefit from their complaint,
30.8% of the students had not investigated whether the product they used had another effect/usage
purpose or not. 72.5% of the students stated that the product they used gave good results for their
usage purpose , 13% stated the product to be harmful for themselves and 72.4% expressed that they
did not consult doctor for the use of herbal treatment. The reason for not consulting a doctor has been
defined as; they did not need it (81.5%), they thought the doctor would react negatively(5,2%), they
were afraid to be criticized(3,0%). There was statistically significant difference between herbal
treatment use and to find herbal treatment use correct, having benefit after the treatment and being

Corresponding Author: TURGAY KOLA, turgay.kolac@inonu.edu.tr


1
NN NVERSTES SALIK HZMETLER MESLEK YKSEK OKULU

1441
informed about herbal treatment (p <0,05). The difference between the use of herbal treatment and the
sex of the students was statistically insignificant (p> 0,05) Conclusion: Among the students it was
detected that; 70,0% of the students believed in alternative treatment, only 14,8% found alternative
treatment incorrect, 52,2% thought both the drugs and plants were safe, 44,1% used herbal treatment.
The herbal products most commonly used by students are products that strengthen the body and
immune system and skin care products. The rates of herbal treatment use has been found to be more in
the groups who found the herbal treatment correct, saw the benefit of the treatment that had already
been used and had been informed about the herbal treatment, and this condition was statistically
significant.

KEYWORDS
herbal treatment, plant, student

1442
Poster Session 11
Submission ID: 1405

INVESTIGATION OF BIONEMATICIDAL VALUE OF SOME


ESSENTIAL OILS AGAINST MELOIDOGYNE INCOGNITA IN VITRO

1 2 1
FATMA GL GZE ZDEMR , BEKIR TOSUN , ARIF ANLI

ABSTRACT
Nematicidal activity of essential oils extracted from 14 plant species representing Apiaceae
family were evaluated in vitro experiments against root knot nematode, Meloidogyne incognita. Fruit
essential oils of Ferulago pauciradiata (Boiss&Heldr), Foeniculum vulgare (Miller.), Ferulago cassia
(Boiss.), Daucus carota (Linne.), Coriondrum sativum L., Conium maculatum L., Artedia squamata L.,
Angelica sylvestris L., Anethum graveolens L., Scandix iberica Bieb., Kundmannia anatolica (Boiss),
Heracleum platytaenium Boiss., Pimpinella anisum L., Smyrnium connatum Boiss&Kotschy were
extracted via hydrodistillation and investigated by gas chromatography-mass spectrometry (GC-MS).
High nematicidal activity was achieved with essential oils from Ferulago pauciradiata (Boiss&Heldr),
Angelica sylvestris L. and Heracleum platytaenium Boiss. Good nematicidal activity was also
obtained with the essential oil from Anethum graveolens L. On the other hand, C. maculatum, S.
connatum, S. iberica and D. carota essential oils yielded weak nematicidal activity. All of the tested
essential oils possessed nematicidal activity against M. incognita and nematode mortality rate ranging
between 39-84.3% on the 3rd day and 59.7-91.7% on the 7th day by essential oil applications. The use
of the crude oils provided satisfactory results at the laboratory level against M. incognita, and needs
further evaluation in greenhouse and field trials.

KEYWORDS
Meloidogyne incognita, essential oils, bionematicide, Apiaceae family

Corresponding Author: BEKR TOSUN, zymbekirtosun@gmail.com


1
SLEYMAN DEMREL UNVERSTY, FACULTY OF AGRCULTURE, PLANT PROTECTON DEPARTMENT
2
SLEYMAN DEMREL UNVERSTY, FACULTY OF AGRCULTURE, FELD CROPS DEPARTMENT

1443
Poster Session 11
Submission ID: 1407

MEDICINAL PLANT MELILOTUS INDICUS (L.) ALL. (FABACEAE)


AND THEIR MICROMORPHOLOGICAL PROPERTIES

1 1 1
ZNUR ERGEN AKN , KRAN ZTRK , TUBA ZBUCAK

ABSTRACT
Medicinal Plant Melilotus indicus (L.) All. (Fabaceae) and Their Micromorphological
Properties znur ERGEN AKN1, kran ZTRK, Tuba ZBUCAK 1 Ordu University,
Faculty of Science and Art, Department of Molecular Biology and Genetics, Ordu,Turkey. University
of Ordu, Ulubey Vocational School, Ordu,Turkey oakcin@gmail.com Melilotus L. (Fabaceae) is
represented with 11 species in Turkey. M. indicus (L.) All. named as ta yoncas, otuzlu yonca in
Anatolia. This species is used as medicinal, forage and nectarius plant. The plant contains coumarin,
which is an anticoagulant. M. indicus species are discutient, emollient, astringent, strongly laxative
and narcotic. The seed is made into a gruel and used in the treatment of bowel complaints and infantile
diarrhoea. The scanning electron microscope (SEM) is an ideal instrument for examining the surfaces.
In this study, we have aimed to investigate leaf, fruit and seed surfaces of M.indicus by SEM. Plant
materials were collected from different localities of Ordu vicinity in flowering period. Taxonomical
descriptions of the specimens were made according to Davis. Dried leaf, seed and fruit samples were
mounted on stubs using double-sided adhesive tape for SEM. Samples were coated with 12.5-15 nm of
gold. Coated samples were examined and photographed with a JMS-6060LV Scanning Electron
Microscope. Taxonomical properties are very important to identification of plant. It is very important
to collect the right plants for the use of medical plants. Close species or subspecies are very similar to
each other. The collection and use of the wrong plant is very dangerous. This means that plants must
be well-recognized and diagnosed correctly. Taxonomical properties are very important to
identification of plant. Micromorphological properties of plant are important taxonomical properties.
In conclusion, micromorphological features of leaves, fruit and seed surface were determined and to
help recognize the M. indicus.

KEYWORDS
Melilotus, M. indicus, Medicinal plant, Micromorphological, SEM.

Corresponding Author: ZNUR ERGEN AKN, oakcin@gmail.com


1
ORDU NVERSTES

1444
Poster Session 11
Submission ID: 1409

INVESTIGATION OF ANTI-CANCER ACTIVITY OF TEUCRIUM


POLIUM L. SUBSP. POLIUM IN DIFFERENT CANCER CELLS

1 1 1 1 1
NURBAHAR FTC , FERDA ARI , OUZHAN AKGN , HULUSI MALYER , SERAP ELKLER KASIMOULLARI

ABSTRACT
Cancer is one of the most important public health problems in our country and in the world.
Although some progress in cancer treatment, new treatments are needed. The vast majority of anti-
cancer agents in clinical use are of plant origin. The research of plant resources that may have
potential as an anti-cancer agent in our country which is rich in plant diversity is very valuable both in
terms of health and country economy. Therefore, complementary therapies of herbal origin have
gained importance in recent years. The aim of the study is to provide an herbal treatment method that
can support cancer treatment. For this purpose, the cytotoxic effects of ethanol extract of Teucrium
polium plant were investigated in vitro in human breast (MCF-7) and lung (A549 and H1299) cancer
cell lines. Cytotoxic activities on the cells were determined by SRB viability test. The death mode
(apoptotic or necrotic) in the cells was evaluated morphological by fluorescence dying. It was
determined that Teucrium polium reduced cell viability as dose-dependent manner (12.5-200 g / ml,
72h) in MCF-7, A549 and H1299 cells. It was determined that IC50 value were calculated as 92 g/ml
(MCF-7), 75.34 g/ml (A549 cells) and 135.66 g/ml (H1299 cells), respectively. According to these
values, the anti-cancer potential of Teucrium polium ethanol extract is higher in A549 cells than the
other. Further analysis is needed for explain the cell death mechanism.

KEYWORDS
Teucrium polium L. subsp. polium, cancer, apoptosis, cytotoxic effect

Corresponding Author: SERAP ELKLER KASIMOULLARI, scelikler@uludag.edu.tr


1
ULUDA NVERSTES, FEN-EDEBYAT FAKLTES, BYOLOJ BLM

1445
Poster Session 11
Submission ID: 1410

SPECIES OF FLEABANE (CONYZA SPP.) IN TURKEY

1 1 1
ALAR MENG , DIDEM CANIK OREL , BRAHIM ZER ELIBYK

ABSTRACT
Species of Fleabane (Conyza spp.) in Turkey Asteraceae family is the biggest family of the
world 1600 genus and 23000 species. Fleabane species (Conyza spp.) are one of the most important
types of Asteraceae family. There are 60 different species of the Fleabane that they generally
distributed on temperate and subtropic areas of all the continents excluding Antarctica. C. canadensis
(L.) known species of Fleabane in Turkey considering that the C. albida Willd. ex. Sprengel, C.
bonariensis (L.) Cronquist ve C. canadensis (L.) Cronquist species exist. Besides the existing types are
quite similar in our country, lamellar structure and inflorescence are the main differentiation criteria
used for diagnosis. Those herbs are used in modern and public medicine because of their rich chemical
metabolites. These plants are used as coagulant, diuretic, expectorant, hypoglycemic, tonic (as
refreshing), antirheumatic, antifungal and antibacterial besides these effects also for dysentry and
hemorrhoid treatment.

KEYWORDS
Conyza spp. Fleabanes, Turkey

Corresponding Author: ALAR MENG, cglr.mngc@hotmail.com


1
ANKARA UNVERSTY, FACULTY OF AGRCULTURE, DEPARTMENT OF PLANT PROTECTON

1446
Poster Session 11
Submission ID: 1412

ANTI-MICROBIAL ACTIVITY OF SALIX BABYLONICA

1 1 1 2 3
ICLAL AGAN , ULKU ALTOPARLAK , SABIHA AYDOGDU , SABAN KORDALI , DEMET CELEBI

ABSTRACT
INTRODUCTION Since the beginning of civilization, humans have used natural products for
healing of different diseases. Salix babylonica (Babylon willow or weeping willow) is a species of
willow native to dry areas of northern China, but cultivated for millennia elsewhere in Asia, being
traded along the Silk Road to southwest Asia and Europe. Salix plant was used in the treatment of
many conditions, including arthritis, menstrual, dental and back pain reduce fevers. and it is used as an
anti-inflammatory drug. This study was conducted to investigate the antimicrobial activity of Salix
babylonica ethanol and acetone extracts on Escherichia coli, Proteus mirabilis, Enterococcus faecalis,
Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans.
MATERIAL and METHOD Minimum inhibitory concentration (MIC) of plant extracts were
determined by the microdilution and disk diffusion method as described by Clinical and Laboratory
Standards Institute. RESULTS Disc diffusion results were ineffective. The MIC values of both extract
(ethanol and acetone) were same except C. albicans, E. faecalis, S. aureus. E. coli, P. mirabilis, S.
epidermidis, P. aeruginosa (>12.500 g/ml, 12.500 g/ml, 97.6 g/ml, >12.500 g/ml for),
respectively. The ethanol extract of Salix babylonica MIC values were determinated for C.albicans
781 g/ml, E.faecalis 781 g/ml. While was ineffective for S.aureus. The acetone extract of Salix
babylonica MIC values were determinated for C.albicans 390 g/ml, E.faecalis 1.562 g/ml, S.aureus
97.6 g/ml CONCLUSIONS Result of this study suggests that salix babylonica extracts can be
effective in tested several microorganisms. Further studies in the effect of different dosages and
duration are suggested. Future studies should elucidate the components responsible for antimicrobial
activity of these extracts against target cultures.

KEYWORDS
Salix babylonica, Antimicrobial activity

Corresponding Author: ICLAL AGAN, iclalagan@gmail.com


1
ATATURK UNVERSTY, SCHOOL OF MEDCNE, DEPARTMENT OF MEDCAL MCROBOLOGY, ERZURUM
2
ATATURK UNVERSTY, FACULTY OF AGRCULTURE, DEPARTMENT OF PLANT PROTECTON, ERZURUM
3
ATATURK UNVERSTY, VETERNARY FACULTY, ERZURUM

1447
Poster Session 11
Submission ID: 1414

ASSESSMENT OF THE ANTIOXIDANT AND REACTIVE OXYGEN


SPECIES SCAVENGING ACTIVITY OF METHANOLIC EXTRACTS
OF ACHILLEA SIPIKORENSIS HAUSSKN. AND BORNM. AND
ACHILLEA TERETIFOLIA WILLD.

1 2
SELVI CNGZ , FERDA CANDAN

ABSTRACT
Oxidative stress is initiated by reactive oxygen species (ROS), which are responsible for
majority of the diseases. However, antioxidants with ROS scavenging ability may have great
relevance in the prevention of oxidative stress. The present study was undertaken, using a methanolic
extracts of Achillea sipikorensis Hausskn. and Bornm. ve Achillea teretifolia Willd, to examine
different in vitro tests in diversified fields including total antioxidant activity, scavenging activities for
various ROS (radicals like hydroxyl, superoxide and hydrogen peroxide), phenolic and flavonoid
contents. Hydrogen peroxide (H2O2) scavenging activity of the methanolic extracts were carried out
following the procedure of Ruch et al. Hydroxyl radicals scavenging activity was measured with
Fenton reaction. Superoxide anion scavenging activity was evaluated by the method of
xanthine/xantine oxidase (XOD) system. Inhibition of reactive oxygen species were calculated for
changing the concentration of plant extracts. IC50 which is the amount of extract supplying 50%
inhibition were calculated using the graph. The ability of the extracts of the Achillea in exhibiting their
total antioxative properties follow the order Achillae spikorensis > Achillae teretifolia. The same order
is followed in their phenolic content, whereas in case of flavonoid content it becomes Achillae
teretifolia > Achillae spikorensis.Miscellaneous results were observed in the scavenging of reactive
oxygen species by the plant extracts, Achillae teretifolia > Achillae spikorensis for hydroxyl and
superoxide radicals, and Achillae spikorensis > Achillae teretifolia for hydrogen peroxide. In a whole,
the studied Achillae extracts showed quite good efficacy in their antioxidant and radical scavenging
abilities, compared to the standards. In conclusion, it may be concluded that methanol extracts of
Achillae spikorensis and Achillae teretifolia as an antioxidant and ROS scavenger; which may be due
to the presence of phenolic and flavonoid compounds. Acknowledgements: This research was part of
the project number F-501 supported by the Research Council of Cumhuriyet University in Sivas /
Turkey.

KEYWORDS
Assessment of the Antioxidant and Reactive Oxygen Species Scavenging Activity of Methanolic
Extracts of Achillea sipikorensis Hausskn. and Bornm. and Achillea teretifolia Willd.

Corresponding Author: SELV CNGZ, selvi.cingoz@gmail.com


1
CUMHURYET NVERSTES
2
CUMHURYET NVERSTES

1448
Poster Session 11
Submission ID: 1415

WHY FERULIC ACID ATTRACTS THE ATTENTION OF MANY


RESEARCHERS?

1 1
FATMA UNAL , DENIZ YZBAIOLU

ABSTRACT
Phenolic compounds possess one or more aromatic rings with one or more attached -OH
groups, which may occur in free, combined, or bound forms that are considered to be part of the
defense mechanisms in plants. Ferulic Acid (FA) (4-hydroxy-3-methoxycinnamic acid-
hydroxycinnamic acid), an Angelica sinensis derived phenolic phytochemical, is a component of
equisetum, angelica, and some Chinese herbal medicines. It is also present in various fruits and
vegetables, such as citrus fruits, bananas, whole grains, spinach, broccoli, eggplants, and cabbage. It
has been reported that FA has numerous physiological functions, including antiinflammatory,
antimicrobial, antifibrosis, and antidiabetic functions. It has a strong antioxidant property which
includes very high hydroxyl radical scavenging activity, superoxide radicals, nitric oxide, and
peroxynitrite scavenging activity. FA has also been found to possess a lot of potential improved effects
related to different diseases. FA can prevent cognitive deficits in mice, attenuate the symptom of
Alzheimer's disease induced by chronic neuroinflammation and oxidative stress in rats, and protect
rats brain after nerve injury induced by cerebral ischemia. Chemopreventive efficacy of ferulic acid in
some kind of carcinogenesis has also been demonstrated. In addition, some publications indicated that
FA produced the antidepressant-like effects. This study was designed to review the health benefits of
phenolic compound FA in in vitro and in vivo studies.

KEYWORDS
Ferulic acid, antioxidant, radical scavenging activity, chemoproventive effect, antidepressant-
like effect

Corresponding Author: FATMA UNAL, funal@gazi.edu.tr


1
GAZ UNVERSTES

1449
Poster Session 11
Submission ID: 1416

INVESTIGATION OF ANTI-CANCER ACTIVITY OF ADANSONIA


DIGITATA (BAOBAB) IN DIFFERENT CANCER CELLS

1 1 1 1 2
KEMAL ZORLI , NADIA RAYMONDE EMMA SAGNA , FERDA ARI , OUZHAN AKGN , ENGIN ULUKAYA ,
1
SERAP ELKLER KASIMOULLARI

ABSTRACT
The Baobab tree (Adansonia digitata) is an indigenous vegetation of several countries in
tropical Africa, including Botswana, Mozambique, South Africa and Namibia. Many parts of the plant,
especially leaves, fruit pulp, seeds and bark fibers, have been used traditionally for medicinal and
nutritional purposes. In addition, antibacterial, antiviral and anti-trypanosome activities have been
reported. An important area of research in cancer treatment is to investigate the anticancer properties
of herbal extracts. For this purpose, cytotoxic effects of ethanolic extract of Adansonia digitata
(Baobab) plant were investigated in human breast (MCF-7) and lung (A549 and H1299) cancer cell
lines in vitro. Cytotoxic activities on the cells were determined by SRB viability test. The death mode
(apoptotic or necrotic) in the cells was evaluated morphologically by fluorescence staining. Adansonia
digitata (Baobab) ethanolic extract was found to reduced viability in the MCF-7, A549 and H1299
cells as dose-dependent manner. Calculated IC50 values were determined to be as 110.83 g/ml in
MCF-7 cells, 36.36 g/ml in A549 cells and 116.41 g/ml in H1299 cells, respectively. We think that
the Adansonia digitata plant has anticancer potential in human lung cancer cells and that its
mechanism needs to be elucidated.

KEYWORDS
Adansonia digitata (Baobab), cancer, apoptosis, cytotoxic effect

Corresponding Author: SERAP ELKLER KASIMOULLARI, scelikler@uludag.edu.tr


1
ULUDA NVERSTES, FEN-EDEBYAT FAKLTES, BYOLOJ BLM
2
STNYE NVERSTES TIP FAKLTES TIBB BYOKMYA ANABLM DALI

1450
Poster Session 11
Submission ID: 1418

A DIFFERENT UTILIZATION AREA OF OLIVE OIL: WOUND CARE

1 1
MERVE YURTTA , KENAN GM

ABSTRACT
A DIFFERENT UTILIZATION AREA OF OLIVE OIL: WOUND CARE Merve
YURTTA1*, Kenan GM2 1University of Amasya, School of Health, Department of Nutrition
and Dietetics, Amasya/TURKEY 2University of Amasya, School of Health, Department of Surgial
Diseases Nursing, Amasya/TURKEY merve.yurttas@amasya.edu.tr ABSTRACT Introduction: The
fact that natural olive oil which is essential component of Mediterranean diet possess compounds with
natural antioxidant properties such as monounsaturated fatty acids, vitamins, carotenoids, phytosterols,
flavanoids, and phenolic compounds both increase its nutritional value and allows its use in medicine.
Method: Literature review was presented extracting from the full texts and summarized articles
reached via databases such as "Pub Med, Scopus, Scince Direct, Ulakbim, Google Scholar" by using
key words such as olive oil, nutrition, and wound care. Results: Olive oil is a highly nutritious
aromatic oil obtained from ripe olive fruits mechanically without applying any chemical treatment.
Olive oil which is stated to have positive effects on health in the literature is also used for treatment
purpose all over the world. It is reported that olive oil has been used in ancient times and todays folk
medicine for wound care by utilizing from its antibacterial and anti-inflammatory effects. It is
expressed to accelerate healing by stimulating the growth factors when it is applied to wound. The fact
that olive oil has antibacterial activity enables olive oil to be an effective agent against bacterial
species, like Klebsiella-Pseudomonas, causing wound infections and having antibiotic resistance.
Conclusion: There are studies examining the effect of olive oil on wound healing and presenting
evidence-based results about this issue. It comes to the forefront in the fields of conventional medicine
and folk medicine due to its clinically important properties such as acceleration of wound healing and
providing the clean environment required for the wound to heal. Key Words: olive oil, nutrition,
wound care

KEYWORDS
olive oil, nutrition, wound care

Corresponding Author: MERVE YURTTA, merve.yurttas@amasya.edu.tr


1
AMASYA NVERSTES

1451
Poster Session 11
Submission ID: 1419

USE OF SPROUT AS ENZYME THERAPY IN PHENYLKETONURIA

1 1
ZLEM KILI , MUSTAFA ERBA

ABSTRACT
Phenylketonuria (PKU) is a hereditary disorder of protein metabolism caused by the
deficiency or absence of the phenylalanine hydroxylase enzyme (PAH) and/or the cofactor of this
enzyme, tetrahydrobiopterin (BH4). An essential amino acid phenylalanine is converted irreversibly
into tyrosine by the PAH enzyme secreted from the liver in healthy individuals. In patients with PKU,
phenylalanine cannot be converted to tyrosine due to the deficiency or absence of PAH and as a result
it accumulates in body fluids and causes damage especially in nerve and brain tissues. Currently,
phenylalanine-restricted diet is the basis of the treatment for PKU patients. In addition, new treatment
approaches have been carried out such as oral phenylalanine ammonium lyase (PAL) enzyme therapy
to improve the quality of life of patients with PKU. PAL converts phenylalanine to trans-cinnamic
acid and ammonia by autocatalytic effect without cofactors. There are studies related to determine the
activity of naturally encapsulated PAL enzyme in plant tissues from various sources such as fruits,
legumes and cereals for patients with PKU. PAL contents and activity of cereals and legumes such as
wheat, corn, green lentil and soybean could be very increased by germination. It was found that the
highest PAL activity was the sprouts of red-spring wheat had on the seventh day of germination. The
stability of PAL activity in wheat sprout during in vitro digestion was also investigated to determine
the potential of enzyme to be used as a dietary supplement for patients with PKU. It is known that the
PAL activity could be preserved to some degree in fresh sprout throughout digestion. Conclusion,
fresh wheat and legume sprouts could be used in diet patients with PKU as oral enzyme therapy.

KEYWORDS
Phenylketonuria, sprout, phenylalanine ammonium lyase

Corresponding Author: ZLEM KILI, ozlemkilic.91@gmail.com


1
AKDENZ NVERSTES

1452
Poster Session 11
Submission ID: 1420

REVIEW OF IMPORTANT INTERACTIONS BETWEEN STATINS


AND HERBAL PRODUCTS OR NUTRIENTS

1 1 2 1 1
ESRA BYK , AYTEN DEMIRCI , MEVRA AL , AYE SAIDE AHIN , BURAK CEM SONER

ABSTRACT
Herbal products have been used for thousand years by human being because of the effects they
have with the purpose of treatment, protection from diseases or improving life quality. Today, usage of
herbal products is still common in the world showing the differences by the aspect of community and
district. A variety of interactions can be seen from the fact that the active components of the herbal
products are the substrates, inducers, and the inhibitors of the same pathways as the drugs in the body.
Also, carrier proteins such as P-glycoprotein (P-gp) and organic anion-transporting polypeptide
(OATP) are assuming an active role on the pharmacokinetics of some xenobiotics; so that, potential of
interaction increases with the drugs transporting through the same transport proteins. Drug interaction
is the change of a drugs effect due to in the presence of another xenobiotic or being under its effect.
Treatment interruption, hospitalization, temporary/permanent disabilities, congenital anomaly, life-
threatening conditions, and death can be seen as a result of these changes. In our study, we are aiming
to evaluate the current data on the interactions of HMG-CoA reductase inhibitors (statins), one of the
group of hypolipidemic drugs, with herbal products and nutrients. Hypolipidemic agents are classified
as statins, fibric acid derivatives, niacin, bile acid sequestrants, and ezetimibe. They are used to
decrease the risk of coronary heart diseases and the other atherosclerotic cardiovascular diseases with
the lifestyle changes by the means of retarding atherosclerosis development but also hindering the
progress. Usage of statins widely and having important clinical drug interactions necessitate the safe
drug use. It is known that statins cause myopathy, elevation on liver enzymes, rhabdomyolysis etc. and
incident of these effects increases after concomitant use with drugs, herbal products or nutrients.
Myopathy which is classified as a serious adverse effect can be seen 1 of 1.000 frequency and
characterized by the elevation of serum creatine kinase at least ten-fold. An interaction that can cause
elevation on the statin plasma levels can increase adverse effects that belong to muscle system such as
myalgia, myopathy, more rarely seen rhabdomyolysis and hematuria. Grapefruit juice increases the
quantity of atorvastatin passing to portal vein via inhibition P-gp and CYP3A4 of which atorvastatin is
a substrate. It is reported that green tea inhibits enzyme CYP3A4, P-gp and OATP carrier proteins.
When green tea is used with simvastatin (one of the substrates of these pathways), it is stated that an
increase of simvastatin blood concentration. St. Johns Wort (Hypericum perforatum)s decreasing
effect on atorvastatin plasma concentration via inducing CYP3A4 causes increase on plasma lipid
levels. It is possible to get benefit from some drug-herbal products interactions as additive. In an
experimental study that was conducted with rats, a significant cholesterol lowering effect with the
combined use of ginger (Zingiber officinale) and atorvastatin was observed. While the levels of
alanine aminotransferase and aspartate aminotransferase increase in the group taking atorvastatin
alone, these enzyme levels are observed less in the combined treatment group. In the consideration of

Corresponding Author: BURAK CEM SONER, burakcemsoner@gmail.com


1
NECMETTN ERBAKAN NVERSTES TIBB FARMAKOLOJ A.B.D
2
KTO KARATAY NVERSTES TIBB FARMAKOLOJ A.B.D

1453
this information by keeping in mind that drug interactions can cause serious results, patient-physician-
pharmacist communication and in this context importance of informing shows up again for the patient
to take the necessary cautions. Especially, almost 50% of patients tend not to indicate the herbal
products or dietary supplements they use gives birth the obligation of assess to this subject more
carefully. In this study, interactions of statins with herbal products and nutrients were searched on the
aspect of clinical studies and case reports; and duration of use, doses/amounts, possible interaction
mechanisms and adverse effects of drugs, herbal products and nutrients were presented as a summary.

KEYWORDS
statins, herbal products, interaction, CYP450, P-gp, OATP

1454
Poster Session 11
Submission ID: 1421

THE EFFECT OF ESSENTIAL OILS ON GERMINATION RATE OF


SOME SUMMERY AND WINTERY WEED SPECIES SEEDS

1 2 1
DERYA T YAVUZ , BURCU BEGM KENANOLU , DERYA KKTA

ABSTRACT
To start germination activity, seeds need water, temperature, oxygen and light depending on
morphological, physiological properties and species. Light, temperature and nitrate ions that are in the
land are alternative factors that can significantly affect seed germination. They are dominant factors in
the enviroment. To speed up or promote germination with organic or inorganic treatments do positive
effects to the seed or seedling. Recently, eco-friendly, clean, cheap, and most important herbal origin
(seaweed extract, medical plant extract, vinegar priming, volatile oils and propolis) treatments use
alternative to chemical-intensive applications. Especially in terms of medicinal and aromatic plants in
our country one of the richest countries in the world. In this study, to determinate different volatile
oils (Mentha longifolia L., Pimpinella anisum L., Carthamus tinctorius L., Citrus limon L.,
Eucalyptus globulus Labil., Lavandula stoechas L. and Prunus dulcis Mill.) effects on some wintery
Sinapis arvensis L., Galium tricornutum Dandy, Melilotus officinalis L. and some summery
Amaranthus retroflexus L., Portulaca oleraceae L., Chenopodium album L. weed speciess seeds
germination rate. Wintery species seeds germinated at15-20 0C and summery seeds germinated at 25-
30 0C in 1 month with petri dishes. At the end of treatments normal-abnormal germination rate (%),
mean germination time (day) determined. Generally, control (untreatment with volatile oil) groups
weed seeds germination rates were between %20-100. The most effective treatments groups are bitter
almond, anise and safflower volatile oils effect on Melilotus officinalis L. ve Amaranthus retroflexus
L. seeds germination rates.

KEYWORDS
Weed seeds, germination rate, volatile oils, priming treatment

Corresponding Author: BURCU BEGM KENANOLU, burcu.kenanoglu@usak.edu.tr


1
UAK NVERSTES ZRAAT VE DOA BLMLER FAKLTES BTK KORUMA BLM
2
UAK NVERSTES ZRAAT VE DOA BLMLER FAKLTES BAHE BTKLER BLM

1455
Poster Session 11
Submission ID: 1422

INVESTIGATION OF TOTAL PHENOLIC AND FLAVONOID


COMPOUNDS OF GIRESUN REGIONS PLANT ORNITHOGALUM
UMBELLATUM WHICH COOKED AND FRESH

1
ELIF APAYDIN

ABSTRACT
It is the study of the total phenolic compounds and total flovonoid compounds of the fresh and
diet of the Ornithogalum umbellatum plant which grows abundantly in Giresun region. Besides, it is
analytical to reveal how much fresh and cooked plant samples are, if there is any difference in the
analysis results. In this study, some of the Ornithogalum umbellatum collected from the nature of
Giresun was cooked by boiling water. The cooked and fresh plant samples were shredded by hand and
extracted with soxhlet in methanol. Subsequently, total phenolics were determined by Folin Ciocalteu
method and total flavonoids were determined by aluminum nitrate method. Qualitative and
quantitative analysis of gallic acid and quarcetin as standard in these two methods was determined by
High Performance Liquid Chromatography (HPLC) method. Plant extracts were determined to contain
a high rate of flavonoids and phenolic substance. In addition total phenolic and total flavonoid
compounds in fresh and cooked plant extracts were observed differences. It was concluded that,
temperature is the reason to been difference in sample result. The results are consistent with studies in
the literature.

KEYWORDS
Ornithogalum umbellatum, fenolic compound, flavonoid compounds, HPLC

Corresponding Author: ELF APAYDIN, eliferenapaydin@gmail.com


1
GRESUN NVERSTES

1456
Poster Session 11
Submission ID: 1423

ANTIMICROBIAL AND ANTIBIOFILM ACTIVITY OF DIFFERENT


EXTRACTS OF CONYZA CANADENSIS L. AND RHODODENDRON
PONTICUM L.

1 2 3 2
TUE NBA , SEDAT SEVIN , ALAR MENG , ENDER YARSAN

ABSTRACT
The emergence of multi-drug resistance in human and animal pathogenic bacteria as well as
undesirable side effects of certain antibiotics has gained attention in the search of new and natural
antimicrobial drugs for controlling the infections. Based on the fact that, development of plant based
antimicrobial drugs has great importance in pharmaceutical industries. In the present study, the
antimicrobial and antibiofilm activities of different extracts of Conyza canadensis(leaves)collected
from Ankara (Trkiye) and Rhododendron ponticum L. (flowers) collected from Ordu (Trkiye)
during the flowering period, were evaluated against clinical isolated microorganisms as well as food
borne pathogens such as Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Candida albicans,
Enterococcus feacalis, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa,
Salmonella enteritis, Staphylococcus aureus. Dried powdered plant materials were extracted with
different solvents (methanol, ethanol, chloroform, acetone and water) and further concentrated to
dryness using a evaporator for comparative analysis. The antimicrobial tests were fist performed using
agar-well diffusion assay. The minimum inhibitory concentration (MIC) and the specific biofilm
formation index (SBF) was then evaluated. Extracts of different solvents showed antibacterial activity
against one or more test bacteria. However, activity against Gram positive bacteria was found more
effective than Gram negative bacteria. Significant differences (p< 0.01) in the activity between the
solvent and water extracts were evaluated. The extracts of organic solvents obtained from
Rhododendron ponticum found to be more effective in the case of bacteria than against fungus. These
differences in potency may be due to the different sensitivity of the test strains. Rhododendron
ponticum caused the major reduction on SBF in dose-dependent manner. The results obtained in this
study appeared to confirm the antibacterial and antibiofilm potential of the plants investigated which
can be an alternative to control strategies or can be used as a model to the search for new drugs.
However, further pharmacological evaluation of refined extracts are needed before they can be used as
therapeutic antimicrobials.

KEYWORDS
Antimicrobial activity, antibiofilm activity, Conyza canadensis, Rhododendron ponticum

Corresponding Author: SEDAT SEVN, sedatsevin59@gmail.com


1
ANKARA UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF BOLOGY
2
ANKARA UNVERSTY, FACULTY OF VETERNARY MEDCNE, DEPARTMENT OF PHARMACOLOGY AND
TOXCOLOGY
3
ANKARA UNVERSTY, FACULTY OF AGRCULTURE, DEPARTMENT OF PLANT PROTECTON

1457
Poster Session 11
Submission ID: 1424

DETERMINATION OF MEDICAL ALTERNATIVE TREATMENT


APPROACHES AND USAGE STATUS OF INDIVIDUALS

1 1 1
PINAR SKLMEZ KAYA , CANAN ASAL ULUS , BAHTINUR TACI , TUBA DBEK, TLAY BOZKURT,
TOLGAHAN AYHAN

ABSTRACT
Aim: This study was made for the purpose of evaulating information ,attitudes and behaviors
of the patients admitted to Samsuns 19 Mays University Hospital for the sake of alternative medical
treatment. Methot: In this descriptive study, 150 individuals (108 women, 72%; 42 men, 28%) who
voluntarily agreed to participate were included in the patients who applied to the Internal Medicine
Clinics at Samsun Ondokuzmays University Hospital. The data were evaluated by the percentage and
chi-square test in the SPSS 20.0 statistical program and P <0.005 was considered significant. Results:
It was determined that 62.7% of the individuals used alternate medical treatment at least once. 30.7%
of the individuals after their illness progressed; 24% after using the medicines recommended by the
doctor, and 22.6% after the use of medicines; 22% reported that they also applied various herbal
remedies without any complaints. According to their declarations, 55.4% reported benefiting from
vegetable products, 34.5% had no effect and 10.1% had negative effects. While 23.3% of the
individuals regarded the herbal products as harmless because they are natural, 1.7% stated that they
think that the herbal products are harmful. The most known alternative non-medical treatment method
was determined as 26.4% of herbal cures and the most used herbal product was ginger with 19.8%.
This was followed by garlic with 18.1%, slimming tea with 14.9%, nettle with 10.9%, ginseng with
9.7%, echinacea with 6.6%, ginkgo with 5.7% and ginkgo with 4.9%. It was found that there was no
statistically significant difference in the frequency of females compared to males (66.6% in females
and 52.4% in males). Again, there was no significant difference between age and educational status
and alternative treatment orientation (p> 0.05). The use of herbal products is 5% in cancer patients,
12% in diabetic patients, 14% in obese patients, 11% in gastric patients, 9% in inflammatory bowels,
16% in skin patients, 2% in urological patients, 11% in female patients, 8% In bile patients and in
other diseases (colds, influenza ) 13%. There was a statistically significant difference (p = 0.04)
between the use of herbal products and the presence of any complaints in the digestive system or liver;
It was determined that as the use of plant product increased, the liver disease increased proportionally.
Conclusion: It was a significant number of individuals used alternative treatment methods. In recent
years , non-medical alternative treatments became increasingly commonly used .In this study, it has
been seen that alternative treatment methods of a significant part of individuals are becoming
increasingly accepted and widely used. For this reason, health workers should be prepared to discuss
these methods with patients in order to minimize the risks and reduce the misconceptions and doubts
of the patients. An environment in which individuals can obtain accurate information about alternative
treatment methods should be established.

Corresponding Author: BAHTINUR TACI, bahtinurtasci@hotmail.com


1
ONDOKUZ MAYIS NVERSTES

1458
KEYWORDS
Alternative medicine treatment, Herbal product

1459
Poster Session 11
Submission ID: 1425

NEUROPROTECTIVE EFFECTS OF THE FRUITS OF LYCIUM


BARBARUM ON ALZHEIMER DISEASE

1 1 1
DEMET NEN , NILFER ACAR TEK , GAMZE AKBULUT

ABSTRACT
Alzheimer's Disease(AD) is a neurodegenerative disease which involves the degeneration or
death of the nerve cells(1). As the average lifespan increases, AD has already become the fourth
leading cause of death in high-income countries(2). One of the main pathological hallmarks of this
disease is the accumulation of the amyloid protein aggregates in the brain that leads to oxidative stress
and inflammation, and cholinergic depletion and excessive glutamatergic neurotransmission are
others(1). And AD highly related to environmental stress, experience of head trauma, daily diet and
exercise, quality of sleep, and air pollution(2). Bioactive food components are physiologically active
constituents in foods or dietary supplements derived from both animal and plant sources, including
those needed to meet basic human nutrition needs, that have been demonstrated to have a role in health
and to be safe for human consumption(3). Lycium barbarum polysaccharide (LBP) has long been
considered to possess anti-apoptotic activities and antioxidant, anti-inflammatory and fertility-
enhancing properties in traditional medical practices in China(4). As a food, dried wolfberries are
traditionally cooked before consumption, used as herbal tea, as well as in Chinese soups, or in
combination with meat and vegetarian meals. Goji fruits are also used for the production of juice, wine
and tincture(3). The chemical composition of goji berry includes monosaccharides(arabinose,
rhamnose, xylose, mannose, galactose, glucose), galacturonic acid and eighteen amino acids,
carotenoids (zeaxanthin, beta-carotene, neoxanthin, cryptoxanthin), flavonoid(quercetin-3-O-
rutinoside, kaempferol-3-O-rutinoside, chlorogenic acid, caffeic acids), taurin, betaine,
vitamins(thiamin, riboflavin, ascorbic acid) potassium, sodium, phosphorus, magnesium, iron and
calcium(3). LBP, the most biologically active fraction of wolfberry, possesses significant antioxidative
and anti-inflammatory effects on multiple tissues(5). Dicaffeoylspermidine derivatives are beneficial
bioactivity constituents responsible for the anti-AD and antioxidant effects of wolfberry. Antioxidant
activities of these constituents could also contribute to antiaging, neuroprotective, and anti-AD effects
of wolfberry(6). Some researchers have demonstrated that a fraction of polysaccharide from Lycium
barbarum provided remarkable neuroprotective effects against beta-amyloid peptide-induced
cytotoxicity in primary cultures of rat cortical neurons(7). Significantly more research is needed but
recent studies suggest that lycium barbarum treatment can be useful for treating memory impairment
induced by several neurodegenerative diseases such as alzheimer disease.

KEYWORDS
lycium barbarum, Alzheimer Disease, neuroprotective

Corresponding Author: DEMET NEN, demetonen@gmail.com


1
GAZ NVERSTES SALIK BLMLER FAKLTES

1460
Poster Session 11
Submission ID: 1426

USE OF DIFFERENT CUMIN DOSES TO IMPROVE THE CHEMICAL


AND SENSORIAL CHARACTERISTICS OF COATED AND FRIED
CHICKEN MEAT

1 1 1 1 1
ELIF AYKIN DNER , ATIKE NUR DURAK , ZLEM KILI , ANDA KO , MUSTAFA ERBA

ABSTRACT
In this research, it was aimed to determine the effect of different cumin doses (0, 1, 2, 3 and 4
g/100 g raw material) in the coating batter formula on quality characteristics of coated fried chicken
meat. The titration acidity, pH, thiobarbituric acid reactive substances (TBARS), total antioxidant
activity (TEAC and ORAC), cooking loss, dimension changes, color parameters (L*, a*, b*) and
sensorial characteristics were determined in the samples. Titration acidity were affected significantly
(P < 0.05) by the cumin dose and it decreased with the increase of cumin dose from 0% to 4%. The
increasing addition of cumin was also efficient for reducing TBARS content and the lowest TBARS
content was determined as 2.13 mol MA/kg in the samples with 4% cumin. Moreover, the TEAC
values of samples significantly increased from 11.07 to 45.02 mol equivalent Trolox/ g dry matter,
while the ORAC value of samples showed only descriptive increase with increasing doses of cumin
from 0% to 4%. Furthermore, the samples with 4% cumin were significantly less yellow and darker (P
< 0.05) (lower L* and a*) than the control group (with 0% cumin) which is thought to be sourced from
increasing cumin dose. In conclusion, the use of 2% cumin in batter formula of coated fried chicken
provides high quality with acceptable sensorial properties because of the highest sensorial points and
is suitable to obtain healthy meat products.

KEYWORDS
Coated meat, chicken, cumin, antioxidant

Corresponding Author: ELF AYKIN DNER, elifaykin@akdeniz.edu.tr


1
AKDENZ NVERSTES

1461
Poster Session 11
Submission ID: 1427

MEDICINAL AND AROMATIC PLANTS USED IN MENOPAUSAL


COMPLAINTS

1 1 1
YEIM ANIK , KAMILE ALTUNTU , EMEL EGE

ABSTRACT
Climacterium is a Greek word and means step of a ladder. This period is a transitional period,
taking place between productivity period and old age, characterized with the symptoms revealing as a
result of differentiating hormonal balance depending on morphological and functional changes in
ovaries. The most important phenomenon in this transition period when various physiological,
psychological and social changes come up is Menopause. Menopause is characterized with decrease in
estrogen secretion, permanent end of menstruation and disappearance of fertility following loss of
ovary functions depending on ageing of ovaries and atresia of follicles. Sudden decrease in production
of estrogen in ovaries in menopause causes vasomotor hot flushes, cardiovascular and gastro-intestinal
changes, cognitive and emotional instability, sleep disorders, changes on skin, urogenital atrophy and
osteoporosis. Life quality, welfare and health perceptions of women may negatively be influenced due
to hormonal originated psychological and physiological changes come up in this period. Hormone
replacement therapy is used to prevent vasomotor symptoms arising in period of menopause and to
prevent short and long term problems of menopause. However, the findings about the fact that
hormone replacement therapy causes increase in the risk of breast cancer, cerebrovascular accident
and cardiovascular disease have reduced use of hormone replacement therapy and directed women to
use alternative treatment methods. The conclusions of the study show that ratio of alternative
treatment usage directed on symptoms of menopause varies between 22% and 83%. Anise, basil,
licorice, dill, parsley, red salvia, dandelion, daisy, Humulus Lupulus, Dong Quai and Hypericum
Perforatum L. and suchlike medicinal and aromatic plants are among the natural estrogen resources
having significant place in reducing menopausal complaint. It is detected that Humulus Lupulus
reduces hot flushes and other menopause signs (sweating, insomnia, heart throb, nervousness)
frequency. Moreover, it is specified that it provides decrease in vaginal dryness when used with E
vitamin combination for urogenital atrophy. It is stated that Dong Quai which is a plant specific to
China, has a supportive effect in menopausal complaints thanks to its efficiency in easing hot flushes
and its moderate estrogenic and vasodilator effect. It is set forth that Hypericum Perforatum L. is
effective in treatment of mild to moderate depression. At the same time, it is ascertained that it
considerably provides decrease in the severity and incidence of psychological, psychosomatic and
vasomotor symptoms for the women with pre and postmenopausal symptoms. In conclusion; being an
important period of life, menopause affects every women in different severities and directs some to
seek treatment. Several women prefer non-pharmacological treatment methods for menopause
complaints because of concerns on side-effects of hormone replacement therapy. It is observed that
medicinal and aromatic plants, efficiency of which is detected with evidence-based studies, are
beneficial for healing menopausal complaints and increasing life quality. Improper and unconscious
use of medicinal and aromatic plants may cause toxic effects. Clinicians need to pursue contemporary

Corresponding Author: YEM ANIK, yesimanik@windowslive.com


1
NECMETTN ERBAKAN NVERSTES SALIK BLMLER FAKLTES

1462
studies with the purpose of ensuring safe use of these plants, need to be aware of the risks and provide
training and consultancy services to the group to which treatment is planned.

KEYWORDS
menopause, complaint, plant, aromatic

1463
Poster Session 11
Submission ID: 1428

THE HEALTH EFFECT OF THE CAROB

1 1 1
DEMET NEN , NILFER ACAR TEK , GAMZE AKBULUT

ABSTRACT
The Carob tree grows in many parts of the Mediterranean region. The fruit of carob
production is nearly 14.000 ton/year and consumed in variously as raw fruit, pekmez or flour in
Turkey(1). The chemical composition of the carob fruit changes vary by two major parts: the
pulp(90%) and the seeds(10%) and depends on cultivar, origin and harvesting time. Carob pulp is rich
in carbohydrate content (35-45%sucrose, 2-4%glucose, 6-7%fructose, 18%cellulose-hemicellulose),
low protein(2-7%), lipid(0,5-1%) and lots of polyphenols, especially highly condensed
tannins(proanthocyanidins), composed of flavan-3-ol groups and their galloyl esters, gallic acid, (+)-
catechin, (-)-epicatechingallate, (-)-epigallocatechingallate and quercetin glycosides(3). Raw carob
pulps mineral composition is rich in calcium, phosphorus, potassium, magnesium, sodium,
manganese, iron, copper and zinc(4). Carobs nutritional and bioactive composition(dietary fiber,
polyohenols, cyclitols) with trace elements that act as cofactors of antioxidant enzymes to protect the
body from oxygen free radicals that are produced during oxidative stres have been linked with the
health-promoting effects such as anti-hyperlipidemia anti-diabetes, anti-diarrheal and anti-
cancer(5).Some studies have found that the concentrated polyphenols extract from carob have
beneficial effect on serum lipids in humans who have hypercholesterolemia. Zunft et al. noted that

in mean total cholesterol and 10.6% in LDL cholesterol(7). Ruiz-Roso et al. found that 4g twice a day
carob fiber intake reduced the t
after 4 weeks(8). Zavoral et al. determined that 30g/day consumption during 2 weeks reduction in
cholesterol 17% and LDL-C 19% in familial hypercholesterolemic children(9). One study found that
total cholesterol (CT), HDL-cholesterol levels significantly increased with the consumption of 20g/day
a non-extractable-tannatesrich carob-fiber for 4 weeks in hypercholesterolemic patients(10). Carob
bean has been used to treat diarrhoeal diseases in Anatolia since ancient times and standard oral
rehydration solution provides effective rehydration but does not reduce the severity of diarrhoea so
Akit et al. tested the clinical antidiarrhoeal effects of carob bean juice(CBJ) and conclude that CBJ
may have a role in the treatment of children's diarrhoea after it has been technologically processed(11).
It has also been shown that the tannins in carob normalized defecation, body temperature, and weight
and cessation of vomiting were reached more quickly by the infants who received 1.5g/kg/day a
tannin-rich carob pod powder (40% tannins or 21.2% polyphenols and 26.4% dietary fiber)(12). The
presence of low glycemic load, high fiber mainly high levels of insoluble fiber and D-pinitol in carob
products could be responsible for the anti-diabetic effects as it regulates blood sugar level in patients
with type II diabetes mellitus by increasing insulin sensitivity(5,13). Banuls et al. study supports that
consumption of carob pod inositol-enriched beverage in prediabetic subjects produces a response that
is dependent on BMI, with a clear improvement of insulin resistance and postprandial and nocturnal
glycemia in non-obese subjects and a marked anti-inflammatory response in obeses(14). Rtibi et al.

Corresponding Author: DEMET NEN, demetonen@gmail.com


1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1464
found that carob pod aqueous extract inhibits intestinal glucose absorption, improves glucose tolerance
and protects against alloxan-induced diabetes in rat(15). Carob is rich in phytochemical
compounds(e.g. quercetin, gallic acid, theophylline etc.) that have antitumor, anti-proliferative and
proapoptotic activity(5). Carob pod and leaf extracts contained antiproliferative agents could be of
practical importance in the development of functional foods and/or chemopreventive drugs(16).
Studies have shown that carob may have health benefits including protecting cancer, reducing
cholesterol, regulating blood sugar level and treating diarrhea symptoms although more studies
needed. The public should be informed and consumption of the raw carob fruit or manufactured
products (e.g. carob pods can be used as a cocoa substitute or coffee cause of not containing caffeine
and theobromine) should be supported.

KEYWORDS
carob, health, antioxidant, anti-hyperlipidemia

1465
Poster Session 11
Submission ID: 1429

ANTIOXIDANT CAPACITY, CHEMICAL COMPOSITION AND


MELLISSOPALYNOLOGICAL CHARACTERIZATION OF
CHESTNUT AND RHODODENDRON HONEYS IN TURKEY

1 1 1
NAZLI MAYDA , ASLI ZKK , KADRIYE SORKUN

ABSTRACT
Introduction: Chestnut (Castanea sativa Mill.) and Rhododendron (Rhododendron sp. L.)
honey are produced generally in Black Sea Region in Turkey and both of them are the special honeys
for their organic component content and known their high antioxidant capacity. Chestnut plant is one
of the important nectar and pollen resources for honey bees. Chestnut honey with dark color and bitter
taste, can stay in a liquid state for a long time because of its slow crystallization rate. Rhododendron
honey, produced by honeybees from the nectars of Rhododendron genus (R. ponticum and R. luteum)
flowers, which are belongs to Ericaceae family. This honeys taste is bitter because of its slightly sharp
taste and most of them contains toxins which are called grayanotoxins and they can be toxic when
their consumption. So people use generally Mad Honey name for this honey due to subsequent
consumption effects. On the other hand this honey is widely used in indigenous medicine. In the first
step of this study we researched the melissopaynological differentiation of the chestnut and
rhododendron honeys and then in the second step we determined the chemical and antioxidant
capacity of the rhododendron, chestnut and mixed chestnut&rhododendron honeys characterization.
Materials and Methods: Total 15 honey samples were collected from 5 different districts [Bartn (n=8);
Kastamonu (n=1); ile (n=1); Amasra (n=2); Dzce (n=3)] from Black Sea Region of Turkey.
Melissopalynological analysis was done by microscope (Olympus CX41). Total phenolic and total
flavanoid capacity were made via UV-Spectrophotometer (Genesys 10S UV-VIS Spectrophotometer).
Chemical composition (Fructose&Glucose and HMF content) was determined by HPLC (Agilent 1200
Series) and GC-MS (Agilent 5973). Results and Discussion: After melissopalynological anaylsis were
obtained 10 monofloral chestnut, 2 monofloral rhododendron and 3 mixed chestnut&rhododendron
honeys. As a result of antioxidant capacity analysis, Total Phenolic contents was determined between
84.02710.0764 and 312.61520,1921 mgGAE/kgSD; Total Flavanoid contents were determined
between 9.67460.0244 and 42.63310.1732 mgQE/kgSD. According to sugar analysis with HPLC,
F/G rates were found between 0.9 and 1.71. GC-MS chemical substance analyses of honeys revealed
aldehydes, aromatic acids, aromatic alcohols, flavanones, esters, aromatic amines and other chemical
substances.

KEYWORDS
Chestnut honey, rhododendron honey, mellissopalynological analysis, chemical analysis,
antioxidant capacity

Corresponding Author: ASLI ZKK, asozkok@gmail.com


1
HACETTEPE NVERSTES

1466
Poster Session 11
Submission ID: 1434

CYTOTOXIC EFFECT OF DIFFERENT EXTRACTS OF SALVIA


CANDIDISSIMA VAHL. SSP. CANDIDISSIMA ON LUNG CANCER
CELLS

1 1 1 2 3
MREN ALOLU , SERAP ELKLER KASIMOULLARI , OUZHAN AKGN , AYEGL CEB , ENGIN ULUKAYA ,
1
FERDA ARI

ABSTRACT
Lung cancer is one of the cancer types that are common in the world today and have a high
mortality rate. More than one million people die each year due to lung cancer. The survival time is 60-
70% (5 years) in the early stage and falls below 5% in the advanced stages. The search for new anti-
cancer compounds is continuing, as there is still no satisfactory success in the treatment of lung
cancer. Several plant-derived drugs are currently used in cancer therapy. In this study, the anticancer
activity of the methanol and ethanol extracts of Salvia candidissima was investigated in human lung
cancer cells (A549 and H1299). The effects of plant extracts on cell viability were determined by SRB
viability test. The apoptotic effect in the cells was observed morphologically by fluorescence staining.
The IC50 values of Salvia candidissima were calculated as 42.9 g/ml in methanolic extract while in
ethanol extract as 32.04 g/ml in A549 cells. In the H1299 cells, the IC50 value of methanol extract
was found as 182.2 g/ml and ethanol extract as 195.8 g/ml. As a results, it will be promising to
investigate the potential of Salvia candidissima extracts to be used as anticancer agents in A549
human lung cancer cells

KEYWORDS
Salvia candidissima vahl. ssp. candidissima, Sitotoksisite, Akcier Kanseri

Corresponding Author: FERDA ARI, ferdaoz@uludag.edu.tr


1
ULUDA NVERSTES, FEN-EDEBYAT FAKLTES, BYOLOJ BLM
2
GRESUN NVERSTES, SALIK BLMLER FAKLTES
3
STNYE NVERSTES, TIP FAKLTES, TIBB BYOKMYA ANABLM DALI

1467
Poster Session 11
Submission ID: 1435

DETERMINATION OF TOTAL ANTIOXIDANT ACTIVITY AND


FLAVONOID CONTENT OF AUBRIETA EKIMII

1 2 3 4
BURCU ARIKAN , RABIYA MERVE CEYLAN , BRAHIM SIRRI YZBAIOLU , NESLIHAN TURGUT KARA

ABSTRACT
Aubrieta, a genus of the Brassicaceae family, is spreading from southern Europe to the Middle
East to Asia. Introduced to the scientific world in 2015, Aubrieta ekimii is an endemic, ostentatious
rock plant in the northwestern Anatolian region with a limited area spread. A. ekimii blooms between
April and May and between the end of May and the month of July it gives fruit. The aim of the study
is to determine the antioxidant activity and flavonoid content of methanol extract prepared from A.
ekimii plant. Antioxidant activity was determined by the TAC Assay kit based on the reduction of
copper. As a result, the total antioxidant activity of methanol extracts of A. ekimii plant was
determined as 786.54 CRE (equivalent to M copper reduction) and the total flavonoid amount was
20.11 2.93 mg catechin equivalent / g sample (KE / g sample). With this recent study, antioxidant
activity and flavonoid content of A. ekimii plant were determined for the first time.

KEYWORDS
Aubrieta ekimii, antioxidant, flavonoid, phenolic

Corresponding Author: NESLHAN TURGUT KARA, neslihantk@istanbul.edu.tr


1
STANBUL NVERSTES/ FEN BLMLER ENSTTS, MOLEKLER BYOLOJ VE GENETK DOKTORA
PROGRAMI
2
STANBUL NVERSTES/ FEN BLMLER ENSTTS, MOLEKLER BYOLOJ VE GENETK YKSEK
LSANS PROGRAMI
3
STANBUL NVERSTES/ ECZACILIK FAKLTES FARMASTK BOTANK ANABLM DALI
4
STANBUL NVERSTES/ FEN FAKLTES, MOLEKLER BYOLOJ VE GENETK BLM

1468
Poster Session 11
Submission ID: 1436

INVESTIGATION OF THE TOTAL PHENOLIC AND TOTAL


FLAVONOID CONTENT OF DIFFERENT FRACTIONS OBTAINED
FROM LIQUIDAMBAR ORIENTALIS MILL. STORAX, FRUIT AND
LEAF

1 2 1 1 3
SMEYRA ETNKAYA , EDA BKER , LKNUR INAR , H. GL DURSUN , PEK SNTAR

ABSTRACT
Liquidambar species (L. orientalis var. orientalis, L. orientalis var. integriloba ve L. orientalis
var. suber) belong to the family of Hamamelidacea. Liquidambar orientalis tree is commonly known
as Sla aac or Gunluk aac in Turkey. L. orientalis is a herbaceous plant known to have
medicinal and cosmetic properties and is widely used in phytotherapy in the Mediterranean region
(Hafizolu 1982). The storax produced by injuring L. orientalis has antiseptic properties (Fernandez
2005). Also it is used as a topical parasiticide, expectorant and for the treatment of some skin diseases
in Turkish folk medicine (Hafizolu 1982). Medical plants which are yielding valuable natural
products are often used in the treatment of various disease (Skrovankova vd. 2012). Many constituents
of medicinal plants are include large amounts of antioxidants such as phenolic compounds, avonoids,
terpenes, tocopherols, and other endogenous metabolites (Zheng ve Wang 2001). In our study, we are
investigated total phenolic and flavonoid contents of different fractions obtained from L. orientalis
storax, fruid and leaf. Total phenolic content with Folin-Ciocalteu colorimetric method and total
flavonoid content with aluminium chloride method carried out. As a result, storax chloroform, fruid n-
hexane and storax methanol fraction have most high level of total phenolic content, respectively. Fruid
n-hexane, storax chloroform and leaf n-hexane fraction have most high level of total flavonoid
content, respectively. References 5. Hafizoglu H. Analytical studies on the balsam of Liquidambar
orientalis Mill. by gas chromatography and mass spectrometry. Holzforschung. 1982;36:3113. 6.
Fernandez, X. (2005). Chemical composition of the essential oils from Turkish and Honduras Styrax.
Flavour Fragrance J. 20: 7073 7. Skrovankova, S.; Misurcova, L.; Machu, L. Antioxidant activity and
protecting health effects of common medicinal plants. Adv. Food Nutr. Res. 2012, 67, 75139. 8.
Zheng, W.; Wang, S.Y. Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food
Chem. 2001, 49, 51655170.

KEYWORDS
L. orientalis, storax, fruit, total phenolic, total flavonoide

Corresponding Author: EDA BKER, eda_buker@yahoo.com


1
NECMETTN ERBAKAN NVERSTES, MERAM TIP FAKLTES, TIBB BYOLOJ A.D., KONYA
2
ANKARA NVERSTES, ECZACILIK FAKLTES, ANALTK KMYA A.D., ANKARA
3
GAZ NVERSTES ECZACILIK FAKLTES FARMAKOGNOZ A.D. ANKARA

1469
Poster Session 11
Submission ID: 1437

IN VITRO ANTIBACTERIAL ACTIVITY OF ESSENTIAL OILS


AGAINST FOODBORNE PATHOGENIC BACTERIA

1 1
NAZAN TOKATLI DEMIROK , MEHMET ALPASLAN

D
ABSTRACT
Essential oils are well known volatile oily liquids. They have recently got scientific interest

TE
owing to their antibacterial, antifungal, antiviral insecticidal and antioxidant properties. Gram- positive
organisms are believed to be more resistant to essential oils than gram-negative bacteria. The purpose
of the present study was to evaluate antibacterial effectiveness of the four essential oil samples which
are Black seed oil (Nigella sativa oil), peppermint oil (Mentha piperita oil), rosemary oil (Rosmarinus
officinalis leaf oil), thyme oil (Thymus vulgaris). The essential oils were obtained by hydrodistillation
EN
using a Clevenger type apparatus. The agar disc diffusion method was employed for the determination
of antibacterial activity of the essential oils and a broth microdilution broth susceptibility assay was
used, for the determination of the minimum inhibitory concentration (MIC). The essential oils were
individually tested against five pathogenic microorgansms: two strains of gram-negative bacteria;
ES
Escherichia coli ATCC 25922, Salmonella Enteritidis ATCC 13076 and three strains of gram-positive
bacteria; Listeria monocytogenes DSM12464, Staphylococcus aureus ATCC6538, Enterococcus
faecalis ATCC51299, containing 108 colony-forming units (CFU) ml-1of bacteria cells. Almost four
oils showed remerkable antibacterial activity against test microorganism. Among the essential oils,
Thymus vulgaris oil and Mentha piperita oil have significant antibacterial activity 38 and 34 mm zone
PR

against Staphylococcus aureus and Salmonella Enteritidis and the lowest MIC value 2.25 mg/ml.
These results suggest the potential of studied these essential oil inhibit microbial growth and they have
potential preserving foods.
T

KEYWORDS
Essential oils, antibacterial properties
O
N

Corresponding Author: NAZAN TOKATLI DEMROK, ntokatli@nku.edu.tr


1
NAMIK KEMAL NVERSTES

1470
Poster Session 11
Submission ID: 1438

THE USE OF OAK ACORNS AS A FUNCTIONAL FOOD IN FOOD


INDUSTRY

1 1
HALUK ERGEZER , RAMAZAN GKE

ABSTRACT
The oak is a kind of hardwood forest tree with 30-35 m heights. There are around 30 oak
species in 18 of them are native to our country. The fruit of all of the oak is called "acorn". Acorns
contains some compounds such as phenolics and tocopherols, as well as tannins as main components,
which can be used in the production of functional foods which vary with the growing period. The
acorn is a nutritious food for humans and animals with approximately 55% starch, 2.75-8.44% protein
and 0.7-7.4% fat content. It has been reported that more than 53-65% the fatty acid profile of acorns
were contained oleic and linoleic acid mixture. Phenolic compounds and tocopherols are the most
important natural antioxidants of acorns. The main tocopherol of acorn was -tocopherol, forming
almost 90% of the total tocopherol content. In addition, 32 different phenolic compounds were isolated
from the acorns, and all of them were reported as ellagic and gallic acid derivatives. It has been
determined that the antiradical scavenging activity values of the isolated phenolic materials were very
high. In some Mediterranean countries, oak acorns are used to give flavor to dessert, ice cream and
some liqueurs. In addition, in Algeria, Morocco and the United States, acorn oil is added to the oil mix
to extend the shelf life of cooking oils. In Spain and Italy, roasted acorns are also used to give an
astringent flavor to breads, cakes and coffee. There are some researches that acorns can be used as a
substitute for synthetic antioxidants, especially in meat, due to high antioxidant capacity of acorns. As
a matter of fact, pigs to be used for Iberian ham production which is specific to Spain have to be fed
with oak acorns. It is known that the antioxidant compounds found in the acorn structure used for the
pig fodder are transferred to the meat structure, thus preventing the product from being oxidized
during the prolonged maturation period.

KEYWORDS
Oak, Acorn, Functional food, Lipid oxidation

Corresponding Author: HALUK ERGEZER, hergezer@pau.edu.tr


1
PAMUKKALE NVERSTES MHENDSLK FAKLTES, GIDA MHENDSL BLM KINIKLI-DENZL

1471
Poster Session 11
Submission ID: 1439

CHARACTERISTICS AND EFFECTIVE USAGE AREAS OF


ROSEMARY

1 1 1 1
GLAH ZEL , ELIF FEYZA TOPDAS , MEMNUNE ENGL , HACER NVER

ABSTRACT
Rosmarinus officinalis (rosemary) is a small needle-tipped, leafy plant belonging to the
Labiatae family. Flowers of rosemary which are white, light blue and blue blooms in spring and
summer. Rosemary does not plow its leaves in winter and has a strong aroma resembling camphor or
eucalyptus odor. It is one of the spices commonly used in European and North American countries due
to its pleasant aroma resulting from its essential oil. Rosemary used as antioxidant or natural
preservative in foods and it is also used in soap, room odor, deodorant, perfume and lotion. Its
essential oils or extracts can be used in meat products, oil-containing foods and oils against oxidation
and rancidity. Antioxidant properties of rosemary are due to carnosol, carnosic acid and rosmarinic
acid. It is reported that the antioxidant activity of carnosic asid is three times higher than carnasol and
seven times higher than butylated hydroxy toluene (BHT) and butylated hydroxy anisole (BHA). It is
recommended that rosemary can be dried and used as an antioxidant source. But besides this usage
areas of rosemary are limited because of intensive taste and odor which are felt even in very diluted
extracts. This problem has been solved by some methods developed in recent years. Especially
commercial rosemary preparations are produced in USA and Japan with colorless, tasteless and
odorless but strong antioxidant effect.

KEYWORDS
Rosmarinus officinalis, Rosemary, antioxidant activity

Corresponding Author: GLAH ZEL, ozellgulsah@gmail.com


1
ATATRK UNVERSTY, FACULTY OF AGRCULTURE , DEPARTMENT OF FOOD ENGNEERNG

1472
Poster Session 11
Submission ID: 1440

KOMBUCHA, THE FERMENTED TEA: ANTIBACTERIAL EFFECTS

1
NAZAN TOKATLI DEMIROK

ABSTRACT

D
Kombucha, is a traditional refreshing beverage that made actually from tea extract
supplemented with sugar and fermented a powerful symbiosis of acetic bacteria and yeasts. The yeast
cells and Acetic acid bacteria use sucrose and glucose and they produce ethanol and acetic acid. Both

TE
ethanol and acetic acid have antimicrobial activity against pathogenic bacteria. This metabolism also
produces vitamins C, B1 B2, B3, B6, B12 and folic acid. The present study was undertaken to
determine the efficacy of Kombucha, a fermented beverage of sugared black tea, [Kombucha was
prepared in a tea broth (10% w/v) supplemented with sucrose (20% w/v)] against five pathogenic
EN
microorgansms: two strains of gram-negative bacteria; Escherichia coli ATCC 25922, Salmonella
Enteritidis ATCC 13076 and three strains of gram-positive bacteria; Listeria monocytogenes
DSM12464, Staphylococcus aureus ATCC6538, Enterococcus faecalis ATCC51299. The agar disc
diffusion method was employed for the determination of antibacterial activity of the Kombucha and a
broth microdilution broth susceptibility assay was used, for the determination of the minimum
ES
inhibitory concentration (MIC). Almost Kombucha showed remerkable antibacterial activity against
test microorganism although Kombucha, showed strong activity against Listeria monocytogenes
DSM12464 which is the highest sensitivity with the largest inhibition zone (20 mm) and the lowest
MIC value (6.25 mg/ml) These overall study results recommend that Kombucha can be used as a
PR

potent antibacterial agent.

KEYWORDS
Kombucha, antibacterial properties
T
O
N

Corresponding Author: NAZAN TOKATLI DEMROK, ntokatli@nku.edu.tr


1
NAMIK KEMAL NVERSTES

1473
Poster Session 11
Submission ID: 1441

TOTAL PHENOLIC CONTENT OF SUBMERGEDLY CULTIVATED


MUCOR MYCELIA

1
BURCU ATLI

D
ABSTRACT
Mucor miehei is commonly used as a producer of aspartic protease and lipase enzymes in food

TE
industry. The aim of the present study was to determine the antioxidant potential of the water extract
of submergedly cultivated Mucor mycelia. The Mucor miehei extract was evaluated for total phenolic
content by Folin's Ciocaltue method and ascorbic acid (vitamin C) concentration by a redox titration
using iodine. Total phenolic content of the Mucor miehei extract was found 546.32 mg/100 g gallic
acid equivalents (GAE). The concentration of ascorbic acid which has antioxidant properties was 1.6
EN
g/100 g. These results indicate that Mucor miehei extract can be considered as promising candidate for
use in medicine and cosmetic industries.
ES
KEYWORDS
Mucor miehei, mycelial extract, total phenolic content, ascorbic acid, antioxidant property
PR
T
O
N

Corresponding Author: BURCU ATLI, burcuatli2006@gmail.com


1
ESKEHR OSMANGAZ NVERSTES

1474
Poster Session 11
Submission ID: 1444

SOME PROPERTIES AND USAGE AREAS OF BASIL (OCIMUM


BASILICUM)

1 1 1 1
MEMNUNE ENGL , ELIF FEYZA TOPDAS , NESLIHAN KAVAZ , GLAH ZEL

ABSTRACT
Basil is known as Basilic by Frenchs and Basilicum by Germans. Also in international trade
Basil, widely used with this name,is used in the ancient Greek with the name 'Basilicos' which means
'king'. Genus Ocimum contains single or perennial species which are belonging to the family of
Lamiaceae. Basil spreads in tropical and temperate regions. In Turkey, it is mainly grown in Western
and Sothern Anatolia. Spice or volatile oil of basil has various uses areas in food industy such as non
alcoholic beverages, bakery products, candies, ice creams, vines and meat products. Also it's volatile
oil which ranges from 0.3% to 1% used in perfumery. The amount of volatile oil and its composition
varies with the species, climate, soil and harvest time. Aromatic compounds found in basil are 1,8
cineol, linalool, citral, methyl chavicol (estragol), eugenol and methyl cinnamate. Species grown in
Africa generally contains camphor. It has been determined that the basil's most common phenolic
substance is navadensin (5,7-dihydroxy-6,8,4'-trimethoxy flavone). This is followed by ladanein,
pilosin, genkwanin, salvigenin, cirsiliol and apigenin. This plant which is rich in phenolic substances
also has a strong antioxidant activity. So it can be use for impart functional properties to some foods. It
has been shown in various scintific studies that basil exhibits antimicrobial activity against Escherichia
coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Salmonella sp., Serratia
marcescens, Klebsiella pneumoniae and Proteus vulgaris.

KEYWORDS
Basil, Ocimum basilicum, antioxidant activity, phenolic

Corresponding Author: NESLHAN KAVAZ, n.kavaz@hotmail.com


1
ATATRK UNVERSTY, FACULTY OF AGRCULTURE , DEPARTMENT OF FOOD ENGNEERNG

1475
Poster Session 11
Submission ID: 1445

BIODEGRADABLE AND EDIBLE FILM-FORMING PROPERTIES OF


SALEP AS A NEW SOURCE

1 1
MGE HENDEK ERTOP , RABIA ATASOY

D
ABSTRACT
The plastic materials made from petroleum are widely used in many industry field because of

TE
their availability, resistance to oxygen, barrier to moisture and reasonable price. However, packaging
materials made from petroleum are not biodegradable and edible. The edible biopolymers obtained
from renewable plant resources are the best option to replace synthetic plastics. Edible films can be
defined as a thin layer placed on or between food components or surface. Edible and biodegradable
films can be used to reduce the migration of O2, CO2 and moisture. Therefore, they improves
EN
appearance and extend the shelf-life of food products. The utilization of edible and renewable sources
from various plants for edible films has been a remarkable issue due to its economic and
environmental impact of them. The ingredients used with this aim can be divided into three categories:
synthetics, lipits and hydrocolloids such as proteins and polysaccharides. Hydrocolloids obtained from
ES
different resources are also the main sources of edible films. Salep is the roots or tubers of
Orchidaceae species which is largely collected in Eastern Mediterranean countries included Turkey.
The tubers of naturally grown orchids are dried and then ground to produce salep powder. Salep is
commonly used as a traditional beverage and a stabilizer for ice cream. Because the glucomannan is
the most important polysaccharide constituent in salep, it is thought to be that salep is very appropriate
PR

raw material for edible and biodegradable films. The film-forming properties of salep is very newly
concept for food industry. Furthermore, there is very limited information about this issue. The main
objective of this review study were to evaluate the edible film-forming properties of salep as a new
source.
T

KEYWORDS
O

Salep, Orchidaceae, glucomannan, edible film, hydrocolloid


N

Corresponding Author: MGE HENDEK ERTOP, mugeertop@kastamonu.edu.tr


1
KASTAMONU NVERSTES MHENDSLK VE MMARLIK FAKLTES GIDA MHENDSL BLM

1476
Poster Session 11
Submission ID: 1446

CYTOTOXIC EFFECTS OF RHODODENDRON PONTICUM L.


EXTRACT ON RAT GLIOMA CELL LINE (F98)

1 1 2 3 1
EMINE KBRA BILIR , SEDAT SEVIN , HIDAYET TUTUN , ERAY ALIIR , ENDER YARSAN

ABSTRACT
Aim: The genus Rhododendron used in traditional medicine for the treatment of inflammation,
pain, cold, asthma, skin and gastro-intestinal disease, is distributed widely around the world.
Rhododendron ponticum L., contains graytonotoxins with diterpene qualities. Increased incidence of
cancer, treatment is costly and create serious side effects applied to people, it becomes necessary to
investigate a scientific alternative treatment and supportive way. The aim of the study was to
investigate in vitro cytotoxic effects of Rhododendron ponticum L. extract whose known amount of
grayanotoxin I and III on common glioma tumors in rat rat glioma cell line (F98). Methods: During
the flowering period of common rhododendrons gathered from the Altinordu District of Ordu and
dried under suitable conditions, extracted with distilled water and lyophilized. The content analysis of
the common rhododendrons was carried out by the Chromatographic Method at Marmara Research
Center Food Institute of the Scientific and Technical Research Council of Turkey. In our study,
cytotoxic activity of different concentrations of the extract of Rhododendron ponticum L.; through
mitochondrial (MTT) and lysosomal (Neutral Red) was evaluated in common glioma tumors using rat
glioma cell line (F98). Results: It was determined that the Rhododendron ponticum L. extract with
grayanotoxin I amount of 55.75 g / kg and grayanotoxin III amount of 7.4 g / kg; had a dose-
dependent cytotoxic effect. IC50 were found as MTT 122,8 and by Neutral red 79,61g/ml,
repectively. Conclusion: Cytotoxicity in glioma indicate that Rhododendron ponticum L. is expected
to be a potential anticarsinogentic activity. Our research on Rhododendron ponticum L. continues on
other cancer cell lines with cytotoxicity tests

KEYWORDS
Cancer cell line, Cytotoxic effect, Grayanotoxin, Rhododendron ponticum L.

Corresponding Author: SEDAT SEVN, sedatsevin59@gmail.com


1
ANKARA NVERSTES VETERNER FAKLTES FARMAKOLOJ VE TOKSKOLOJ ANABLM DALI
2
MEHMET AKF ERSOY NVERSTES, VETERNER FAKLTES, FARMAKOLOJ VE TOKSKOLOJ
ANABLM DALI
3
ANKARA NVERSTES, VETERNER FAKLTES, PATOLOJ ANABLM DALI

1477
Poster Session 11
Submission ID: 1447

MARINATION TECHNOLOGY IN SEAFOOD, SOME AROMATIC


PLANTS USED AND IMPACT MECHANISMS

1 2 3 2
MEHTAP BAYKAL , AYGL KKGLMEZ YANDIM , HLYA SAYI , MEHMET ELK

ABSTRACT
Marination is the application of fresh, frozen, salted fish or fish parts by treating them with
acetic acid or other organic acids and salt without heat effect. In addition to the development of new
products with the improvements in food processing methods, it is also aimed to extend the durability
of the products obtained and to protect the qualities. Through the marination, food products that are
abundant in certain periods are provided for human consumption even during periods when they are
less frequent. These products can be served to consumers with flavors such as sauce, cream,
mayonnaise, oil, as well as various spices and herbs. In the marination technology, it is widely used
such as bay laurel, dill, estergon, mustard seeds, whole cloves, black pepper, red pepper, allspice,
cumin, ginger, rosemary, thyme, basil, tarhun and capari. The acid taste of marinades is softened with
herbs and spices and thus a balanced taste is being tried to be formed. The herbs used in the marinating
process are used to the products not only to add taste, flavor and aroma but also to minimize the
negative odors that may occur. Spices and herbs used have protective effect or minimized lipid
oxidation. Spices also mask color and smell rather than inhibition of deterioration. In some studies it
has been found that these herbs stabilize the quality of the product and prevent the development of
harmful bacteria. It is also necessary to pay attention to the qualities of the spices added to the
solution, because at this stage some bacterial and proteolytic enzymes in the solution are still effective.
Depending on the storage temperature, it can cause bad odor development, color loss, softening. In
this review emphasizes the importance of various herbs that enhance taste and endurance in marination
technology.

KEYWORDS
Marination, seafood, aromatic plants, herbs, spice

Corresponding Author: MEHTAP BAYKAL, mbaykal@cu.edu.tr


1
UKUROVA NVERSTES YUMURTALIK MESLEK YKSEK OKULU TURZM VE OTEL LETMECL,
LOKANTA VE KRAM HZMETLER BLM
2
UKUROVA NVERSTES SU RNLER LEME TEKNOLOJS
3
UKUROVA NVERSTES YUMURTALIK MESLEK YKSEK OKULU BTKSEL VE HAYVANSAL RETM

1478
Poster Session 11
Submission ID: 1448

EFFECTS OF ETHEPHON TREATMENT AS SOIL DRENCH ON


PLANT HEIGHT AND QUANTITATIVE PROPERTIES OF NATIVE
NARCISSUS GROWN IN POTS

1 1
SEVIM DEMR , FISUN GRSEL ELKEL

ABSTRACT
In this study, the effects of ethephon treatment as soil drench on plant height, quantitative and
other properties of native narcissus (Narcissus tazetta L.) grown in pots were investigated. When
plants were 7-10 cm tall, ethephon at 0, 250 and 500 ppm were applied as soil drenches. The effects of
ethephon treatment on plant length, leaf length, the time of flowering, number of flower and the flower
life were determined. In addition, quantitative measurements (leaf area ratio, specific leaf area, leaf
thickness, leaf weight ratio and stem weight ratio) were analyzed in native narcissus. When narcissus
were grown in pots in the greenhouse reached to the sale stage plants were taken to the laboratory at
20 C to evaluate the postproduction life and quality of pot plants. The shortest plant height was
obtained from the 500 ppm ethephon treatment, plant height was 7.67 cm with %51 shorter than
untreated control. Ethephon application also shortened the leaf length up to %20. The shortest leaf
lenght (14.55 cm) was obtained from 500 ppm ethephon treatment. The ethephon treatment decreased
leaf area ratio and specific leaf area, but increased the leaf thickness and leaf weight ratio compared to
the control plants. The effects of treatments on plant height continued in laboratuary (home-office)
conditions after production. The shortest plant height (12.62 cm) was obtained from 500 ppm
ethephon treatment whereas the height of untreated control plants were 24.25 cm during the post
production life of pot plants.

KEYWORDS
Narcissus, plant height, soil drench, ethephon, quantitative measurements

Corresponding Author: SEVM DEMR, dmrsevm@gmail.com


1
ONDOKUZ MAYS UNVERSTY, AGRCULTURAL FACULTY, DEPT. HORTCULTURE, ATAKUM, SAMSUN

1479
Poster Session 11
Submission ID: 1449

INVESTIGATION OF THE TOTAL PHENOLIC AND FLAVONOID


CONTENT OF SOME FRACTIONS OBTAINED FROM ROOT AND
STEM OF RHEUM RIBES (LIGHTED)

1 2 1 1 3
ILKNUR INAR , EDA BKER , SMEYRA ETINKAYA , HATICE GL DURSUN , PEK SNTAR

ABSTRACT
In order to struggle metabolic diseases and cancer cases, it is becoming necessary to use
complementary medicines, phytotherapotics and appropriate food supplements besides dietary and
lifestyle intervention. The incorporation of phytochemical complex mixtures of different chemical
structures, such as phenolic acids, polyphenols, flavanoids and terpenoids, often has the advantage of
targeting a number of molecular pathways involved in the pathobiology of complex diseases, with a
significant reduction in toxic side effects. Rheum Ribes belongs to the family Polygonaceae, which is
called as "lively, fluffy or fuzzy" among the people, especially in the subtropical and temperate
regions of the world, especially in the east of Turkey, Iran, Iraq, Lebanon to Afghanistan and Pakistan,
Is a plant species that spreads between slopes. R. Ribes (juvenile shoots and leaf stalks) are commonly
used against diarrhea, stomach aches and nausea, as well as measles, flowers, hemorrhoids and bile
removers. It is thought that strong active compounds possessed by this plant are based on all these
properties. The total flavonoid content of root methanol, root ethylacetate, root hexane and stem
methanol, stem ethylacetate, stem hexane fractions of Rheum ribes were studied by aluminum chloride
colorimetric method. According to the results obtained, the highest values were found in the hexane
fraction of stem, methanol, ethylacetate, trunk hexane fractions, and root ethylacetate fractions in root
methanol, root ethylacetate and root hexane fractions. Total phenolic content was determined by Folin-
Ciocolteu method. According to these test results, the highest value was found in the fraction of
methanol, stem ethylacetate, trunk hexane fractions, fraction of ethylacetate, fraction of methanol,
radical ethylacetate, and fraction of radical hexane fractions

KEYWORDS
Rheum ribes, total phenolic, total flavonoide

Corresponding Author: LKNUR INAR, ilknurcinar@msn.com


1
NECMETTN ERBAKAN NVERSTES, MERAM TIP FAKLTES, TIBB BYOLOJ A.D., KONYA
2
ANKARA NVERSTES, ECZACILIK FAKLTES, ANALTK KMYA A.D., ANKARA
3
GAZ NVERSTES ECZACILIK FAKLTES FARMAKOGNOZ A.D. ANKARA

1480
Poster Session 11
Submission ID: 1450

NEMATICIDAL ACTIVITY OF ESSENTIAL OILS DERIVED FROM


APIACEAE FAMILY AGAINST ROOT LESION NEMATODE,
PRATYLENCHUS THORNEI

1 1 2 1
BEKIR TOSUN , ARIF ANLI , FATMA GL GZE ZDEMR , HAMIDE BAKIRKAYA

ABSTRACT
Pratylenchus spp. is a plant pathogenetic nematode which is migratory endoparasites that feed
and reproduce in the root and move around, responsible for root lesion disease on many host plants in
temperate regions around the world. Fruit essential oils of Heracleum platytaenium Baiss,
Hippomarathum microcorpum (Bieb.) Fedtsch., Ferulago cassia Boiss and Chareophyllum byzantinum
Boiss belonging to Apiaceae family of Turkey flora in Isparta, were screened for their in vitro
nematicidal activity against the Root Lesion Nematode, Pratylenchus thornei. The essential oils were
isolated by hydrodistillation and investigated by gas chromatography mass spectrometry (GC-MS). A
total of 37, 42, 38, 47 components of the H. platytaenium, H. microcorpum, F. cassia and C.
byzantinum essential oils were identified, respectively. High nematicidal activity was achieved with
essential oils from F. cassia and C. byzantinum which caused 93.46% and 90.93% mortality. H.
microcorpum was found to be more effective than H. platytaenium. The dominant components of the
effective oils were chrysanthenyl acetate (17.4%), mesitaldehyde (5.8%), limonene (15.3%) and -
pinene (13.6%) in F. cassia, Methylebenzoate (44.9%), l-Limonene (38.4%) in C. byzantinum, beta.-
Myrcene (21.9%), cis-Ocimene (17.8%), beta.-Phellandrene (12.0%), bicyclogermacrene (8.2%) in H.
microcorpum and 2-ethylhexyl acetate (41.4%) and E4-dodecenylacetate (17.0%), octilin (7.7%) in H.
platytaenium. Nematicidal activity of the essential oils investigated in the research against P. thornei is
reported for the first time. The essential oils and their main components described here in merit further
study as potential nematicides against the Root Lesion Nematode.

KEYWORDS
Pratylenchus thornei, essential oil, nematicidal activity, Apiaceae

Corresponding Author: BEKR TOSUN, zymbekirtosun@gmail.com


1
SLEYMAN DEMREL UNVERSTY, FACULTY OF AGRCULTURE, FELD CROPS DEPARTMENT
2
SLEYMAN DEMREL UNVERSTY, FACULTY OF AGRCULTURE, PLANT PROTECTON DEPARTMENT

1481
Poster Session 11
Submission ID: 1451

USAGE OF COLORANT PLANTS IN KONYA'S FELTMAKER ART

1 2
ETIN ZTRK , SALIH GIRGI

ABSTRACT
Due to its climate and geographical position, the fact that the livestock breeding has been
carried out in Konya plain for centuries has enabled the development of carpets and plain weaving.
The fact that Konya is a very rich region in terms of colorant plants and insects is also an important
influence on the formation of these arts. The first raw materials of natural colorant are some stone, soil
and mining varieties and some molluscs and insect varieties with staining properties. However,
awareness of color diversity in plants has led to the use of whole or a part of the plant in dyeing.
Hundreds of different varieties of colors and shades of different colors have been used in carpet art,
Felt art, Ceramic art and many other cultures. The colors of the plant, which are obtained by using
different regions and applying different methods, have been preserved as a secret and the generations
are transferred with great confidentiality. In this study, the use of plant dyes and coloring methods in
Konya Felt art were investigated.

KEYWORDS
Felt, Wool, Konya, Colorant Plants

Corresponding Author: ETN ZTRK, cetinozturk2001@gmail.com


1
NECMETTN ERBAKAN NVERSTES
2
SELUK NVERSTES

1482
Poster Session 11
Submission ID: 1452

EVALUATION IN TERMS OF HABITAT FEATURES OF SOME


TRUFFLE SPECIES : CASE OF AKILLI, VIZE

1 2
HSEYIN KABA , HSEYIN HILESIZ

ABSTRACT
Truffle exploration activities is done in collaboration of Marmara Forestry Research Institte
and stanbul Regional Directorate in terms of General Directorate of Forest Truffle Action Plan
2014-2018" and Projectless Works. It is protected founding in the truffle as without dogs and with
trained dogs at akll town, Vize Subdistrict Directorate, Vize Forest District Directorate in terms of
Action Plan. Natural Truffles is usually found at altitudes about 250 m, at age of development of
bc, inside mixed stands of Cedar (Cedrus libani A.Richard), Oak (Quercus petraea (Matt) Liebl and
Quercus robur L.), Black pine (Pinus nigra J.F. Arnold) and Hornbeam (Carpinus betulus L.), at
akll town. According to soil analysis result in plot at points natural truffle, it is found pH 6,00-7.85
; EC (S/cm) 295-769 ; CaCO3 % 0,22-33,44 ; N % 0,14-1,96 ; C % 7,73-19,03. Tuber aestivum
Vittad. was detected in July of 2015 and in akll town. Species of Tuber burumale Vittad. and Tuber
borchii Vittad. were found at the beginning of February and March of 2017 in the forest of akll
town.

KEYWORDS
Truffle, Habitat, akll town, Vize

Corresponding Author: HSEYN KABA, huseyinkaba@ogm.gov.tr


1
MARMARA ORMANCILIK ARATIRMA ENSTTS, STANBUL
2
ORMAN BLGE MDRL, STANBUL

1483
Poster Session 11
Submission ID: 1453

COMPOSITION AND ANTIMICROBIAL ACTIVITY OF THE


ESSENTIAL OIL OF ONOSMA MALATYANA BINZET FROM
TURKEY

1 2 3 4 5
GN BNZET , RIZA BNZET , ERSAN TURUNC , AYLIN DOGEN , HAKAN ARSLAN

ABSTRACT
Onosma malatyana Binzet is an endemic species growing in Turkey. This work aimed to
investigate to chemical composition of essential oils obtained from Onosma malatyana Binzet roots
and their antimicrobial activities against Staphylococcus aureus (ATCC 25923), Streptococcus
pneumoniae (ATCC 10353), Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 29212),
Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 25853), Candida albicans (ATCC
10231) and Candida glabrata (ATCC 4322). The essential oils from roots were obtained two different
methods, which are hydrodistillation and Soxhlet extraction. Soxhlet extraction was carried out in
three solvents with different polarities such as petroleum ether, methanol and ethyl acetate. The
essential oils and volatile components of roots were identified by Gas chromatography-mass
spectrometry system. We used the Kovats indices, mass spectra and standard compounds to
determination of essential oils. The major constituents were: for hydrodistillation, abietatriene
(16.97%), manool (15.44%), (Z,Z)-10,12-hexadecadienyl acetate (12.42%), geranyl acetone (9.06%);
for Soxhlet extraction in petroleum ether, 2-isopropyl-1H-permidine (21.26%), eicosanal (11.84%),
sertindole (8.32%); for Soxhlet extraction in methanol, isopimpinellin (15.91%), methyl
dihydromalvalate (12.00%), butanoic acid (8.87%), palmitin,2-mono- (8.00%), 2-propylfuran (7.63%);
and for Soxhlet extraction in ethyl acetate, xanthatin (15.28%); manool (12.60%). Furthermore, the
antimicrobial activity of the extracts was evaluated using modification microdilution methods.
According to the antimicrobial results, all extracts were more susceptible to Candida albicans.
Acknowledgement: This study was supported by the Research Fund of Mersin University in Turkey
with Project Number: 2015-AP3-1199.

KEYWORDS
Onosma, Essential oil, Soxhlet extraction, hydrodistillation extraction, Antimicrobial activity.

Corresponding Author: GN BNZET, polatgun@gmail.com


1
MERSN UNVERSTY, DEPARTMENT OF ELEMENTARY SCENCE EDUCATON, FACULTY OF EDUCATON,
MERSN UNVERSTY, 33160, MERSN, TURKEY
2
MERSN UNVERSTY, SCENCES AND ARTS FACULTES, DEPARTMENT OF BOLOGY, 33343, MERSN
3
MERSN UNVERSTY, SCENCE AND TECHNOLOGY APPLED AND RESEARCH CENTER, MERSN
UNVERSTY, 33343, MERSN, TURKEY
4
MERSN UNVERSTY, DEPARTMENT OF PHARMACY, FACULTY OF PHARMACEUTCAL MCROBOLOGY,
MERSN UNVERSTY, 33160, MERSN, TURKEY
5
MERSN UNVERSTY, DEPARTMENT OF CHEMSTRY, FACULTY OF ARTS AND SCENCE, MERSN
UNVERSTY, 33343, MERSN, TURKEY

1484
Poster Session 11
Submission ID: 1454

INVESTIGATION OF THE EFFECIENCY OF NATURAL


ANTIOXIDANTS IN DOGS FOOD

1 1 1 1
FATMA NAL , MUSTAFA SELUK ALATA , OUZHAN KAHRAMAN , ABDULLAH ZBLGN , MUSTAFA
2 3
ULUDA , EREF NAL

ABSTRACT
This study was conducted to determine the efficacy of natural antioxidant substances in dog
food. Food was prepared in extruded form, balanced with vitamins and minerals containing poultry
meal, rice, barley, corn, vegetable and animal fat. On the dry matter basis, 25% crude protein, 8%
ether extract, 3% crude fiber and 6% ash were found in food. Seven antioxidants were added food with
oil. 10mg/kg BHA was used as control. Vitamin C(E300), vitamin E(E306) and citric acid(E330)
20mg/kg, rosemary essential oil 1.5g/kg, Meat Plus (YUMESA; black cumin, allspice, fennel, thyme,
ginger, cumin, salt) and mixture of special spices (Bilyem; sumac, garlic, cumin, red pepper, etc.)
0.5g/kg. Each group food was divided into 12 pieces and placed in airtight bags. Half of the foods
were kept in refrigerator and other half was kept in closet, which wasnt exposed to light at room
temperature. At 1st, 2nd, 3rd, 4th, 5th, and 6th months of incubation period, a part of each antioxidant
group that were kept at room temperature and in refrigerator, was ground. TBA and peroxide value
analyzes were performed as 2 replicates. It was concluded that TBA and peroxide value analyzes in
determining oxidation level in food were parallel and foods kept in refrigerator less oxidized. It was
observed that oxidation levels were similar in foods kept in refrigerator during 1st and 2nd months,
and the oxidation level gradually increased in groups added with rosemary essential oil, vitamin C and
Vitamin E after 3 months. It was determined that the oxidation levels of rosemary essential oil,
Vitamin C and Vitamin E added groups which were kept in room temperature were very high
compared to others from 1th until 6th month. From third month on, oxidation levels in BHA and citric
acid groups increased. It was determined that addition of 2 herbal mixtures was able to maintain
oxidation level at low level for 6 months at room temperature(P<0.01). As a result, it was concluded
that YUMESA and Bilyem herbal mixtures can be successfully used as antioxidants in dog food

KEYWORDS
Dog food, antioxidant, herbal mixture, oxidation

Corresponding Author: FATMA NAL, fatmaaksakalinal@gmail.com


1
SELUK NVERSTES, VETERNER FAKLTES, HAYVAN BESLEME VE BESLENME HASTALIKLARI
ANABLM DALI, KONYA
2
BLYEM GIDA SAN. TC. LTD. T., ANKARA
3
SELUK NVERSTES, VETERNER FAKLTES, ZOOTEKN ANABLM DALI, KONYA

1485
Poster Session 11
Submission ID: 1456

APPLICATIONS OF PRIVATE FORESTRY IN TURKEY AND USAGE


POSSIBILITIES OF NON-WOOD FOREST PRODUCTS IN THIS
CONTEXT

1 2 1
HAMIDE GBBK , SADETTIN GLER , RECEP BALKI

ABSTRACT
Forests covers 27% of the surface area of Turkey and almost all of them are owned by state.
Only < 0.1% of all forest areas (about 18 000 ha) were owned by privately. This is mainly due to
different geographical and ecological characteristics of forests and approximately 7.6 million villagers
whose life dependency to forest products. This dependency complicates direct privatisation of forest
areas and transition to private forestry. On the other hand, there are some applications that can be
considered in Forestry Privatization. These applications are forests owned by private or legal persons,
Private Afforestation Areas, Forests Recreation areas, Forest areas suitable for utilization of natural
Non-wood Forest Products (NWFP) and urban forests can be accepted among these applications.
Natural forest areas that are rich in NWFP and privatization of forests established via private and
industrial afforestation may support the transition to private forestry. Within this concepts, in addition
to the rapid growing species such as poplar, alder, and eucalyptus, walnut, chestnut, pistachio, almond,
spruce, terebinth, carob and olive are also used as NWFP. These circumstances have continued to
increase in recent years. In this review study, the current status of Turkey's forests, changes in the
understanding of private forestry from past to present and recent applications on private afforestation
have been investigated and comments and suggestions on the process to private afforestation have
been made.

KEYWORDS
Private Forestry, Private Afforestation, Non-wood Forest Products, The areas of utilization
from NWFP, Recreation areas, Urban Forests

Corresponding Author: HAMDE GBBK, gubbuk@akdeniz.edu.tr


1
AKDENZ NVERSTES ZRAAT FAKLTES BAHE BTKLER BLM / ANTALYA
2
ORMAN GENEL MDRL BATI AKDENZ ORMANCILIK ARATIRMA ENSTTS MDRL /
ANTALYA

1486
Poster Session 11
Submission ID: 1457

ANTIOXIDANT AND PHENOLIC CHARACTERISTICS OF AIR DRIED


GINGERS (ZINGIBER OFFICINALE): EFFECT OF ULTRASOUND
PRE-TREATMENT.

1 1 1
AYSUN YURDUNUSEVEN YILDIZ , SAMI GKHAN ZKAL , ZLEM ZAMBAK

ABSTRACT
Antioxidant and Phenolic Characteristics of air Dried Gingers (Zingiber officinale): Effect of
Ultrasound Pre-treatment. Aysun YURDUNUSEVEN YILDIZ, Sami Gkhan ZKAL, zlem
ZAMBAK* Pamukkale University, Engineering Faculty, Food Engineering Department, Denizli,
Turkey *Presenting Author: . ZAMBAK (ozambak@pau.edu.tr) Abstract The objective of this study
was to discover the effect of Ultrasound (US) pre-treatment application before drying on antioxidant
and phenolic characteristics of ginger (Zingiber officinale). For this purpose, ultrasonic probe with 20
kHz frequency was used. Before US application, ginger samples were cut into size of 1 cm x 1 cm x
0,5 cm. Ultrasound pre-treatment was carried out in distilled water with 100 % amplitude during 5, 10
and 20 minutes. The ratio of sample to water was 1:4 (w/v). After ultrasonic pre-treatment ginger
samples were placed in drying oven trays. Drying experiments were carried out in hot air drying oven.
Drying was performed at 0.3 m/s air velocity and at 60C drying temperature. For determination of
antioxidant activity and phenolic compounds, ginger samples were collected at every 10 min during
the drying period and the collected samples were stored at -18C until being used in the analyses. For
the extraction of phenolics, 1 g of ginger samples was mixed with 10 mL of aqueous methanol. The
ratio of water to methanol was 50:50 (v/v). The mixture was sonicated for 10 min in an ultrasonic
bath, followed by mechanical shaking for 15 min at room temperature. After the centrifugation of the
mixture at 9000 rpm at 4 C for 15 min, supernatants were collected into amber vials. Total phenolic
content and antioxidant capacity of these extracts were performed. Total phenolic contents of ginger
extracts were determined with the Folin- Ciocalteu reagent using gallic acid as a standard. Results
were expressed as milligrams of gallic acid equivalent (GAE) per gram of dried ginger weight. 2,2-
Diphenyl-1-picrylhydrazyl (DPPH) assay was used to determine the antioxidant activity of ginger
extracts. Results were expressed in mmol Trolox equivalent (TE)/g dried ginger weight. Antioxidant
capacity of the ultrasound pre-treated samples was found higher than the untreated samples in the first
40 minutes of the drying. After that time antioxidant capacity of ultrasound pre-treated samples was
found lower than the untreated samples. Generally antioxidant capacity of the all samples was
decreased during drying, but antioxidant capacity of the ultrasound pre-treated samples was decreased
fast in compared to untreated sample.It is thought that the ultrasound pretreatment breaks down the
cells and allows the antioxidant substances to be released from the cells. So antioxidant capacity of
ultrasound pre-treated samples was high in first minutes of drying, but antioxidant substances released
are considered to be tending to oxidation and may have been oxidized during the drying, therefore the
antioxidant capacity of the ultrasound pre-treated samples decreased fast. On the other hand phenolic
compounds of US pre-treated ginger samples were found less than the untreated samples. The

Corresponding Author: ZLEM ZAMBAK, ozambak@pau.edu.tr


1
PAMUKKALE NVERSTES

1487
deformation of the cell structure by the application of ultrasound resulted in the phenolic components
released and degraded more rapidly by the effect of heat.

KEYWORDS
Ultrasound, Drying, Ginger, Antioxidant Capacity, Phenolic Compounds

1488
Poster Session 11
Submission ID: 1459

MODELLING OF ANTIMICROBIAL CHANGE IN SOME HERBAL


TEA BAG DURING THE BREWING

1 1 1 1
ENGIN DEMIRAY , HALIL BRAHIM KAYA , ELIF TADELEN , TLIN YILMAZ

D
ABSTRACT
In this study, Sage, linden and chamomile herbal tea bags purchased from national markets

TE
were used. The herbal tea bags are first placed in cups and freshly boiled hot water is added. Brewing
time for herbal tea bags was determined as 10 minutes and samples were taken for kinetic studies at
specific times (1.min, 3.min, 5.min, 7.min and 10.min). Well diffusion method was used to determine
antimicrobial activity. Micrococcus luteus was used as the indicator bacteria. As a result of the
analyzes, it was determined that the change of antimicrobial activity was first kinetic model.

KEYWORDS
EN
Herbal Tea Bag, Antimicrobial, Kinetic, Modelling.
ES
PR
T
O
N

Corresponding Author: ENGN DEMRAY, edemiray@pau.edu.tr


1
PAMUKKALE UNVERSTY

1489
Poster Session 11
Submission ID: 1460

EFFECTS OF SOME ESSENTIAL OILS ON GERMINATION AND


SEEDLING GROWTH IN DRY BEAN (PHASEOLUS VULGARIS L.)

1 1 1 1 1
MUHARREM KAYA , RUZIYE KARAMAN , EBRU DNLER , AYKUT ENER , NAZMI ELK

ABSTRACT
This study was carried out field experiments in SDU Agricultural Research and Application
Center and germination experimentals in laboratories of Field Crops Department in 2016/2017. The
aim of study is to determinethe effects of some essential oils on the germination and some seedling
growth characteristics in dry bean varieties. It was carried out field experiments according to
completely randomized blocks design split plot; laboratory experiments according to completely
randomized plots split plot design with 3 repetitions. It was used eker, Sarkz and Burgan of bean
varieties as seed material; commercial essential oil preparations derived from fennel, garlic and thyme
plants as essential oils in the study. When the aphids were started to show on plants in the field
experiments, the solutions prepared with origanum (Origanum onites) (3 ppm), fennel (Foeniculum
vulgare) (10 ppm) and garlic (Allium sativum) (10 ppm) were sprayed to the green plants. Also,
insecticide containing Deltamethrin (at 50 ml / da dose) was used as a chemical control. In the
laboratory experiments, germination tests were carried out seeds of the bean varieties with the
solutions containing the thyme (0, 1, 2 and 3 ppm), fennel and garlic (0, 2, 4, 6, 8 and 10 ppm)
essential oils. The germination rate, mean germination times, seedling and root lengths and seedling
and root weights were examined in seeds obtained from field experiments (only pure water was
applied) and laboratory tests. While the application of thyme oil in seeds obtained from field
experiments is negative in relation to germination and seedling growth characteristics in Sarkz and
eker varieties; it effects the positive in the Burgan variety. It is determined that garlic and fennel oils
howed a positive increase in the germination and seedling growth characteristics of the varieties
except Sarkz variety. In laboratory tests, doses of 1 and 2 ppm of thyme oil and doses of 2, 4 and 6
ppm of garlic oil were found having positive effects on the examined properties in all bean varieties.
All doses of fennel oil caused reduced on germination and seedling growth.

KEYWORDS
Bean, Essential Oils Germination, Seedling Growth

Corresponding Author: MUHARREM KAYA, muharremkaya@sdu.edu.tr


1
SLEYMAN DEMREL NV. ZRAAT FAKLTES

1490
Poster Session 11
Submission ID: 1461

EFFECTS OF PLANTING TIMES ON SURVIVAL RATE AND PLANT


DEVELOPMENT OF CAROB (CERATONIA SILIQUA L.) SEEDLINGS
IN MARGINAL AREAS

1 2 1
RECEP BALKI , SADETTIN GLER , HAMIDE GBBK

ABSTRACT
There are no reports and apparently studies on planting times of carob plants for non-irrigated
marginal lands in the world. Therefore, in this research, the effects of planting times on survival rate
and performances in terms of growth of stem diameter and plant height in field conditions were
examined. The study was conducted in marginal land located in Antalya-Hurma region and
observations were carried on between 2014 and 2016. One year old carob seedlings propagated from
wild locust seeds were used in this study. Seedlings were planted in two different months, December
and January and no irrigation were made throughout the research including time of planting. Planted
seedlings were taken into plastic shelters for protection from hot summer droughts in June.
Measurements for plant diameter (mm) and height (cm) were made at planting and thereafter with 3
months periods. Plant survival rate of plant were determined in October 2016. There was no planting
time effect determined on survival rate of seedlings statistically. Although it was not statistically
significant, the survival rate was higher in December planted seedlings compared to that found in
January planted ones. Similarly, plant diameter and height values were higher in December planted
ones as well.

KEYWORDS
Carob, Ceratonia siliqua, Seedling survival rate, Seedling development, Marginal areas

Corresponding Author: RECEP BALKI, recepbalkic@akdeniz.edu.tr


1
AKDENZ NVERSTES ZRAAT FAKLTES BAHE BTKLER BLM / ANTALYA
2
ORMAN GENEL MDRL BATI AKDENZ ORMANCILIK ARATIRMA ENSTTS MDRL /
ANTALYA

1491
Poster Session 11
Submission ID: 1462

NEW EXTRACTION METHODS USED IN THE PRODUCTION OF


MEDICINAL AND AROMATIC PLANT EXTRACTS

1 2
ZLEM ZAMBAK , SAMI GKHAN ZKAL

ABSTRACT
New Extraction Methods Used in the Production of Medicinal and Aromatic Plant Extracts
zlem ZAMBAK, Sami Gkhan ZKAL* Pamukkale University, Engineering Faculty, Food
Engineering Department, Denizli, Turkey *Presenting Author: S.G. ZKAL (sgozkal@pau.edu.tr)
Medicinal and aromatic plants have become very important in recent years and our country has an
important place in terms of medicinal and aromatic plants. These plants are used in many sectors such
as; pharmacology, medical science and food science due to their benefits. Extracts of these plants can
be obtained by various methods and these extracts can be used in many sectors. Extraction process is
applied in different forms according to the final product to be obtained from these plants. In generally
extracts is obtained from medicinal and aromatic plants by passing a solvent through the solid raw
material. In these methods, solvent penetrates into the plant material during extraction to dissolve the
components having similar polarity. The main factors affecting the quality of extracts are; part of the
plant used, solvent used and extraction method. When these factors are correctly selected, the yield of
extraction increases. In recent years some researches has been carried out on new extraction methods
to increase extraction efficiency. Supercritical and subcritical fluid extraction, Microwave assisted
extraction, Ultrasonic extraction, Pressurized liquid extraction, Solid-phase extraction methods are
some of these new extraction methods. In this study these new extraction methods used in the
production of medicinal and aromatic plant extracts were discussed.

KEYWORDS
Medicial plants, Aromatic plants, Exraction

Corresponding Author: SAM GKHAN ZKAL, sgozkal@pau.edu.tr


1
PAMUKKALE UNVERSTY, ENGNEERNG FACULTY, FOOD ENGNEERNG DEPARTMENT

1492
Poster Session 11
Submission ID: 1463

THE USAGE OF PLANT EXTRACTS AS ANTIOXIDANT AND


ANTIMICROBIAL AGENT IN MEAT AND MEAT PRODUCTS

1 1 1
SAMI GKHAN ZKAL , HALUK ERGEZER , ZLEM ZAMBAK

ABSTRACT
The Usage of Plant Extracts as Antioxidant and Antimicrobial Agent in Meat and Meat
Products Sami Gkhan ZKAL, Haluk ERGEZER, zlem ZAMBAK* Pamukkale University,
Engineering Faculty, Food Engineering Department, Denizli, Turkey *Presenting Author: .
ZAMBAK (ozambak@pau.edu.tr) Lipid oxidation and microbial spoilage are the are the main factors
that determine food quality loss and shelf-life reduction. The growth of microorganisms in meat
products may cause spoilage or foodborne diseases. On the other hand lipid oxidation leads to loss of
flavor, texture and color of meat products. Although synthetic additives have been widely used in the
meat industry to inhibit both, the process of lipid oxidation and microbial growth, the trend is to
decrease their use because of the growing concern among consumers about such chemical additives. In
recent years different strategies have been developed in order to improve the quality of meat and meat
products so the formulations of meat products are modified by different methods. One of these
methods is the addition of natural plant extracts to the product. In that way lipid oxidation can be
delayed and microbial safety can be provided. In recent years there has been an increase in the number
of researches on natural additives. It is reported that in many cited literature tea, green tea, rosemary,
artichoke, sage, onion, citrus bark, pomegranate, carob, white grape etc. extracts are used as
antioxidant or antimicrobial agents in meat and meat products. The use of natural additives as
antioxidants and antimicrobials will be beneficial in terms of improving quality of meat and meat
based products. In this study plant extracts used as antioxidant and antimicrobial agent and their
effects on the quality of meat and meat products are discussed.

KEYWORDS
Antioxidant, Antimicrobial, Plant extracts, Meat

Corresponding Author: ZLEM ZAMBAK, ozambak@pau.edu.tr


1
PAMUKKALE NVERSTES

1493
Poster Session 11
Submission ID: 1465

PANAX GINSENG: PHARMACOLOGICAL PROPERTIES AND DRUG


INTERACTIONS

1 2 3 1
BURAK CEM SONER , MEHMET MESUT PIKIN , MEVRA AL , AYE SAIDE AHIN

ABSTRACT
Ginseng is one of the most popular herbal products used for thousands of years. Many species
and varieties of ginseng are used. Asian ginseng (Panax ginseng) and American ginseng (Panax
quinquefolius) are two of the best-known species in the world. Both contain different groups of the
steroidal saponins called ginsenosides or panaxosides, as the major component. However, Siberian
ginseng (Eleutherococcus senticosus Maxim.) that is also marketed as ginseng is a completely
different plant of the Araliaceae family and its constituents are chemically different. Ginseng is used to
enhance the bodys resistance to stress and to improve mental and physical performance. It has also
been used for diabetes, insomnia, sexual inadequacy, for degenerative conditions associated with
ageing, to improve healing and as a stimulant. In vitro studies using isolated pancreatic islet cells have
shown that ginsenosides stimulate insulin secretion independent of extracellular calcium. In addition,
in vivo studies in rats showed that P. ginseng extract increases the number of insulin receptors in bone
marrow and reduces the number of glucocorticoid receptors in the rat brain. Ginsenosides inhibit the
reuptake of many neurotransmitters into brain synaptosomes in rats (in descending order: gamma-
aminobutyrate, noradrenaline, dopamine, glutamate, serotonin). P. ginseng has been shown to increase
interferon production in vitro and in vivo and increases killer cells and antigen-dependent cytotoxic
activity in human peripheral lymphocytes. Ginseng saponins have (-) chronotropic and (-) inotropic
effects with a similar mechanism of action to verapamil. Some in vitro studies has also reported
increased coronary blood flow and (+) inotropic effects. Theoretically, ginseng may interact with
cardiovascular system drugs. According to experimental studies, P. ginseng inhibits angiotensin-
converting enzyme activity but does not affect nitric oxide production. The effects of ginseng about
opioids are confusing. Ginseng blocks the analgesic effect of opioids. However, it potentiates the
antinociceptive effect of pentazocine and aspirin. Also, it has been shown that ginseng prevents
tolerance to opioids and psychostimulants in rats. In a mice study, it has been shown that saponins in
ginseng increased pain threshold significantly. In their study they have speculated that saponins in
ginseng might be opioid like peptide receptor agonist without addiction side reactions. P. ginseng may
interact with monoamine oxidase inhibitors. P. ginseng has been reported to cause fever, headache,
tremors and insomnia when used in combination with phenelzine. According to in-vitro and animal
studies, ginseng preparations can show synergistic activity with cytotoxic drugs, chemotherapy and
radiation. In vitro studies suggest that panaxytriol obtained from P. ginseng can produce a synergistic
effect with mitomycin-C. According to in vitro studies, P. ginseng can synergistically interact with
paclitaxel and reduce cisplatin-induced nausea and vomiting. Ginseng is a nonspecific central nervous
system (CNS) stimulant and theoretically, increases the effects and adverse effects of prescription and

Corresponding Author: AYE SADE AHN, aysesaide@gmail.com


1
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ AD.
2
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES ROLOJAD.
3
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ AD.

1494
non-prescription CNS stimulant medications. Also, it may interact with sedative drugs. According to a
clinical study in 36 patients with type-II diabetes, ginseng significantly reduces blood glucose and
hemoglobin-A1c levels. Therefore, attention should be taken when used with antidiabetic drugs.
Ginseng may stimulate the release of the adrenocorticotropic hormone, which may increase plasma
cortisol levels. Theoretically, P. ginseng may have an additive effect when taken with sildenafil. Based
on in vitro studies, camphor ingredient of ginseng is able to inhibit drug efflux and CYP3A4 mediated
metabolism of HIV protease inhibitors. Ginseng preparations may have additive effects when used in
combination with antiviral drugs such as rimantadine, amantadine, zanamivir and oseltamivir. Ginseng
has side effects such as diarrhoea, loss of appetite, nausea and vomiting. This may alter the efficacy of
gastrointestinal agents.

KEYWORDS
Panax ginseng, drug interaction, Asian ginseng

1495
Poster Session 11
Submission ID: 1467

ANTI MICROBIAL EFFICACY OF CURCUMIN AND ITS POTENTIAL


CONTRIBUTION TO THE ECONOMY

1 2 3
SERKAN EN , MERVE EN , SEFA ELK

ABSTRACT
Curcumin is a natural compound which grows in India and South East Asian regions and is
obtained from the rhizomes of Curcuma longa plant. Curcumin is commonly used as anti-
inflammatory. Curcumin has been studied for a variety of clinical practices for many years. According
to researches, it has therapeutic effects on many inflammatory diseases, including diabetes, and
different types of cancer. Curcuminin fungi have been shown to have antimicrobial potential against a
broad spectrum of microorganisms including numerous Gram positive and Gram negative bacteria.
Curcumin has been demonstrated to exhibit anti-infective activity against virulence, quorum sensing
and biofilm formation in Pseudomonas aeruginosa infections. According to the official report
published by the Association of Research-based Pharmaceutical Companies (AIFD) in 2006, the total
drug market in our country has reached 9.9 billion dollars and antibiotics are ranked as the first by
20% within this cost. Similarly, according to the intellectual property rights protection index for the
year 2015 of the Property Rights Alliance (PRA), Turkey is ranked as the 58th among 129 countries.
As it is understood from this report, the drug in Turkey is behind the world in terms of competition
with the number of local patents. In the light of all this information, the development of national
antimicrobials is important in limiting the cost of antibiotics that are ranked as the first among our
national drug expenditure expenses.

KEYWORDS
Curcumin, anti microbial effect, economy

Corresponding Author: MERVE EN, mervesen@mu.edu.tr


1
AFYON KOCATEPE NVERSTES, ATATRK SALIK HZMETLER MESLEK YKSEKOKULU
2
MULA SITKI KOMAN NVERSTES, FETHYE SALIK HZMETLER MESLEK YKSEKOKULU
3
AFYON KOCATEPE NVERSTES, TIP FAKLTES, TIBB BYOKMYA A.D.

1496
Poster Session 11
Submission ID: 1468

FOENICULUM VULGARE: PHARMACOLOGICAL PROPERTIES


AND DRUG INTERACTIONS

1 2 1 3 1
AYTEN DEMIRCI , MEVRA AL , AYE SAIDE AHIN , HILAL KO SONER , BURAK CEM SONER

ABSTRACT
Foeniculum vulgare Mill. is a medicinal and aromatic plant with a lifetime of two years and
belongs to the family of Apiaceae (Umbelliferae). The oil (Foeniculi aetheroleum) and seed like fruit
(Fennel fruit/ Foeniculi fructus) of Foeniculum vulgare Mill. are used in medicine. Fennel fruit
includes sweet fennel (Foeniculum dulce) and bitter fennel (Foeniculum vulgare). They both contains
trans-anethole, fenchone, and estragole in different proportions. Fennel fruits are used as carminative,
Fennel oil is used as an aromatic carminative. The main properties of fennel and fennel oil are derived
from their carminative and expectorant activities. Anethole effects smooth-muscle motility in digestive
tracts; higher doses have antispasmodic effects. A dose-dependent reduction in the density of
respiratory secretions (bronchosecretolysis) occurs. Fenchone has antimicrobial and fungicidal effects
in vitro. Fennel seed has a spasmolytic effect on the smooth muscles and accelerates the vibration rate
of the ciliary epithelium of the bronchial mucosa (secretomotor action). In vitro studies shows that
fennel has antimicrobial, gastric motility enhancing, antiexudative, and presumably antiproliferative
effects. Fennel also shows estrogenic activity which seems to have some benefits in amenorrhea and
dysmenorrhea. Fennel changes the oral absorption of quinolone antibiotics due to metal cation
ingredients. Concurrent use with ciprofloxacin significantly changes absorption, distribution and
elimination of ciprofloxacin after oral administration. Fennel reduces bioavailability of ciprofloxacin.
In an in vitro study, 13 components that has an inhibitory effect on human CYP3A4 enzyme were
isolated from methanol extract of fennel. From these, 5-methoxypsoralen (5-MoP) showed the
strongest inhibition. Also, 5-MoP is an important component of grapefruit juice that has in vivo
inhibitory effect on CYP3A4 which is well studied. Fennel can cause inhibition of intestinal CYP3A4
which is the most expressed CYP enzyme in the intestine. Intestinal CYP3A4 inhibition has been
shown to cause a clinically significant increase in plasma concentrations of drugs such as felodipine
and midazolam. Co-administration of birth control medicines containing estrogen (ethinyl estradiol,
levonotgestrel ethinyl estradiol and estradiol/norethindrone) with fennel may reduce the efficacy of
contraceptives. Tamoxifen is a nonsteroidal antiestrogen used in breast cancer. Combination with
fennel may reduce the effect of tamoxifen. Long-term use of the fennel as a laxative may result with
hypokalemia thus it may potentiate the effect of cardiac glycosides and may interact with
antiarrhythmic drugs that restores sinus rhythm such as quinidine and medicinal products that prolong
QT. Using with other drugs that causes hypokalemia (eg, diuretics, adrenocorticosteroids) may
increase the risk of electrolyte imbalance.

Corresponding Author: BURAK CEM SONER, burakcemsoner@gmail.com


1
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ AD.
2
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ AD.
3
KONYA DR.FARUK SKAN KADIN DOUM VE OCUK HASTANES

1497
KEYWORDS
Foeniculum vulgare, fennel fruit, drug interactions

1498
Poster Session 11
Submission ID: 1469

USING OF POPLAR (POPULUS SPP.) SEED HAIR FIBERS FOR SEA


PETROLEUM SPILL

1 2
ERCAN VELOLU , FILIZ TRKSEVEN VELOLU

D
ABSTRACT
Sea routo transportation is more preferability way because of economic and environmentally

TE
friendly than other options but sea routo may cause environmental accident that is increasing of
polluted sea. Naturally sea water is ripple and flowy so oil spill is easly spread water serface. Sea
water pollitions are not only effect the pollutant starter point but can easly poluted far pleaces of
accident. Principal sea polutants are liquid petroleum and oil product. Petroleum and oil products have
exhibited hydrocarbon features so sea water characteristics spoil and sea ecosiystem affect badly from
EN
oil polutant. Sea life is demaged from oil spill as well pollition is quickly spreaded of sea surface by
winds and sea flows. Such as oil spill of Deepwater Horizon in 1989 and 2010 at Alaska. Main oil
producing countries of north and south hemisphere use Turkey bosporus routo for oil trade. The
stanbul and anakkale Bosporuss are vital importand status for The Black Sea and The
ES
Mediterranean Sea countries. 9006 Tankers passed the bosporuss of Turkey in 2013. 2292 Tankers
were lading 117.1 billion ton petroleum and oil product. In 1979 and 1994 two big sea accident were
occured at Turkey Bosporus. The accedents were cause heavy pollition. Oil absorbents are hydrofobic
and preferability oil absorbents substrats which can be organic, inorganic and syntetics. Absorbents
can change depend on the aim of environmetal disturbance. Genaraly absorbents use afectively at sea
PR

side and small roundup poll. Absorbent substance not suitable for the open sea. Absorbent substance
which use scramble petroleum polition can cause secondery politunt for environment. Absorbents are
use cerafully and limited. Choosing of absorbent is important for buoyancy, saturation, oil retention,
strength and durability, fermentation, costs and availability, storage and transportation properties. The
genus Poplar, which includes poplars are world wide known visual and fast growing tree species.
Poplar seeds can cause some problems at city life. A tree have 280 000- 14 850 000 seed that can
T

cause problem. Seeds have good properties as a petroleum and heavyy oil ansorbance. The absorption
values of 180-211 g heavy oil/g fiber and 55e60 g heavy oil/g fiber respectively, surpass all known
O

natural absorbents. Poplar seed hair fibers and cotton are obtained an extremely promising natural
source for the production of oil super absorbents. Because of cotton is a hydrophilic substance , it can
N

be sang in water. Poplar seed hair fibers is hydrophobic. Hydrophobic substance is preferability for
petroleum and heavy oil pollutions. Slovenian National Building and Civil Engineering Institute tested
poplar seed hair fibers and other polar anda apolar substance. Poplar seed hair fibers can treat polar
solvents (diesel fuel, row petroleum, hexane, petroleum ether, cloroform, tetrahydrofuran) from water
surface. It has patent international. Poplar seed hair fibers are not harmfull for human and
environment.

Corresponding Author: ERCAN VELOLU, ercanvelioglu53@gmail.com


1
KAVAK VE HIZLI GELEN ORMAN AALARI ARATIRMA ENSTTS MDRL
2
KOCAEL L GIDA TARIM VE HAYVANCILIK MDRL

1499
KEYWORDS
Poplar seed fibers, petreleum spill, sea pollution treatment, natural absorbent

1500
Poster Session 11
Submission ID: 1470

AQUATIC PLANTS AS ALTERNATIVE SOURCES TO MEDICAL AND


AROMATIC PLANTS

1 1 1 1 2
HATICE TEKOUL , AYSUN KOP , ALI YILDIRIM KORKUT , HLYA SAYI , HARUN DIRAMAN

ABSTRACT
Usually aquatic plants (macrophytes) are photosynthetic organisms in microscopic (algae) and
macroscopic (macro algae) structures that live in fresh water and sea. Aquatic plants have been used as
human food from antiquity to daily life and in our time they have begun to be used as alternative
sources in various fields such as health, industry and agriculture by introducing different
characteristics through scientific researches. Spirulina and Chlorella are used in the health food market
as powders, capsules, tablets, or pastilles, while marine macroalgae are used as antibiotics, antiviral,
anti-inflammatory, cytotoxic and antimycotic besides their use as nutrients. As in marine plants,
freshwater plants are also used as human food, aquaculture and animal feed, and all parts of these
plants (root, rhizome, tuber, seed etc.) are evaluated in various fields such as medicine, compost and
building materials. For example; Watercress (Nasturtium ofcinale) is used as diuretic, antiscorbutic,
laxative and Water spinach (Ipomoea aquatic) is also used for food poisoning, headache, hematuria,
hemorrhoids and cough. The purpose of this study is to evaluate the information on the use of aquatic
plants spreading in fresh waters, particularly in the field of medicine, by examining previously
published national and international publications.

KEYWORDS
aquatic plants, alternative source, health industry

Corresponding Author: HARUN DIRAMAN, hdiraman@aku.edu.tr


1
EGE NVERSTES, SU RNLER FAKLTES, YETTRCLK BLM, BORNOVA-ZMR
2
GIDA KONTROL ARATIRMA VE UYGULAMA MERKEZ, AFYON KOCATEPE NVERSTES

1501
Poster Session 11
Submission ID: 1471

ZINGIBER OFFICINALIS: PHARMACOLOGICAL PROPERTIES AND


DRUG INTERACTIONS

1 2 3 1
BURAK CEM SONER , MEHMET MESUT PIKIN , MEVRA AL , AYE SAIDE AHIN

ABSTRACT
Ginger has been widely using as a cooking spice and medicinal plant from ancient times in
India and in China. Today ginger is part of the folk medicine as well as in modern medicine. It is used
for the treatment of nausea and vomiting in pregnancy and for prevention of travel and sea sickness.
Many countries have approved ginger as a nonprescription drug for the prevention of motion sickness.
It is also recognized with its anti-inflammatory effect in treatment of rheumatoid arthritis and
osteoarthritis, being on par with many steroidal preparations. The essential oil of Rhizoma Zingiberis
(Ginger) include -zingiberene, ar-curcumene, -bisabolene, neral, geranial, (E)--farnesene and
zingiberol. Pungent compound (gingerols and shogaols), diarylheptanoids (gingerenones A and B),
vitamins and %50 starch are also present. Fresh ginger root contains gingerols, shogaols, 6-
dehydrogingerdione, and galanolactone as the major constituents. 6-gingerol is the main pungent
component of dried ginger. 6-gingerol can convert to 6-shogaols due to dehydration of 6-gingerols.
Fresh ginger is used as antiemetic, antitussive, expectorant, and for inducing perspiration and dispel
cold, whereas dried ginger is used for stomachache, vomiting, and diarrhoea accompanied by cold
extremities and low pulse, resolve phlegm retention, for cough and dyspnea with copious frothy
expectoration and for abnormal uterine bleeding. In vitro studies have shown that fresh ginger extract
inhibits both cyclooxygenase and lipoxygenase. Inhibition of arachidonic acid metabolism results with
platelet aggregation and inhibition of prostaglandin and leukotriene production. 6-gingerol, 10-
dehydrogingerdione and 10-gingerdione are the main ingredients responsible from these effects.
According in vitro studies, these components inhibit prostaglandin synthesis more potently than
indomethacin. The chemical structures of gingerols shows partial similarities with prostaglandins.
Gingerols have been found to be potent inhibitors of prostaglandin biosynthesis. 6-gingerol reduces
nausea and vomiting by increasing motility. Galanolacton, similar with ondansetron, has an anti-
emetic effect via serotonin (5HT-3) receptors located in ileum. Antiserotonergic activity of ginger
including 6-, 8- ve 10-gingerols has also been shown by in vitro studies. Dried ginger is shown to be
useful in rheumatoid arthritis. More than 75% of arthritis patients who consumed ginger rhizome
powder experienced analgesic effects and reduction in joint swelling. Gingerols have been reported to
be hypoglycemic in diabetic rats. Ginger can interact with antacids, H2 antagonists and proton pump
inhibitors by its potential in increasing stomach pH. The high dosage of ginger may cause central
nervous system (CNS) depression and theoretically increases the effect of barbiturates,
benzodiazepines and CNS depressants. Ginger may have a dose-dependent inotropic activity and
theoretically may interact with positive inotropic agents and beta-blockers. In diabetic rats serum
glucose levels are significantly lowered due to the hypoglycemic effect of ginger, which may

Corresponding Author: AYE SADE AHN, aysesaide@gmail.com


1
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ AD.
2
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES ROLOJ AD.
3
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ AD.

1502
theoretically increase the effects of hypoglycemic drugs. Ginger is a potent thromboxane synthase
inhibitor, which prolongs bleeding time. Patients using warfarin and other medicines that affect
platelet activity should avoid taking ginger supplements. Ginger may prevent clotting. Concomitant
use with drugs like aspirin, clopidogrel, diclofenac, ibuprofen, naproxen, dalteparin, enoxaparin,
heparin, warfarin may further reduce clotting and may increase risk of bleeding. Ginger may increase
the absorption, bioavailability and elimination half life of metronidazole so combined use may
increase side effects of metronidazole.

KEYWORDS
Zingiber officinalis, drug interactions, ginger

1503
Poster Session 11
Submission ID: 1472

ETHNOBOTANICAL USES OF SOME MEDICINAL PLANTS ON THE


ASTERACEAE FAMILY FROM HATAY (TURKEY)

1 1
FARUK KARAHAN , AHMET LM

ABSTRACT
Hatay city hosts rich biodiversity, including about 225 taxa of belonging to Asteraceae family.
The present study reveals the ethnobotany and traditional medicinal uses of 23 taxa belonging to 16
genera of the Asteraceae family in Hatay province (East Mediterranean of Turkey). The study was
mainly focused on the medicinal plants used for treatment of various ailments/diseases by the local
people in the city. The data on plants included botanical names, vernacular names, the parts used and
specific purpose of use. The richest genus are; Centaurea L. (4 taxa), Achillea L., Anthemis L.,
Artemisia L. and Taraxacum F. H. Wigg. (2 taxa each). Local people generally use herbals as areal
parts (45.1%), flowers (16.1%), capitulum and leaves (12.9% each). The common preparation of the
medicinal plants in the museum are drink as tea (48.8%), external (20.9%) and infusion (16.3%).
Local people in the region commonly use for the remedy of gastrointestinal system disorders,
respiratory system disorders, genital-urinary system disorders, skin diseases and metabolism disorders.
Our results were comparatively discussed with the other literatures. We believe that fnding of this
study will significantly contribute to the ethnobotanical studies at local or regional scales.

KEYWORDS
Ethnobotany, Medicinal plants, Folk medicine, Asteraceae, Hatay

Corresponding Author: FARUK KARAHAN, farukkarahan34@gmail.com


1
MUSTAFA KEMAL NVERSTES BYOLOJ BLM

1504
Poster Session 11
Submission ID: 1473

CAMELLIA SINENSIS: PHARMACOLOGICAL PROPERTIES AND


DRUG INTERACTIONS

1 2 1 1
ESRA BYK , MEVRA AL , AYE SAIDE AHIN , BURAK CEM SONER

ABSTRACT
Green tea is produced without fermentation from the leaves of Camellia sinensis belonging to
Theaceae family. Black tea or red tea is processed by fermentation and heating, whereas oolong tea is
partially fermented. Green tea contains more catechin than black tea and oolong tea. The presence of
high amounts of catechins, essential minerals and vitamins are responsible for high antioxidant
potential of this tea. Tea contains 5% caffeine and 24% tannins with minor amounts of other xanthines
such as theophylline and theobromine. Its tea also contains flavonoids. Green tea appears to contain
greater quantities of the flavonol-type flavonoids than black tea. Minor flavonols (10%), polymeric
flavonoids (20%) and epigallocatechin-3-gallate (EGCG) are the major constituents of about 50-80%
of the green tea catechins. Leaf buds and fresh tea leaves are used as stimulant and diuretic due to its
caffeine content. Also they are used as an astringent for gastrointestinal disorders which they effect via
polyphenols and tannins they contain. In addition, green tea extracts are used in the treatment of
genital warts. Tea is generally preferred as a drink. Green tea extract is a rich polyphenol source. The
phenolic components of green tea extract have strong antioxidant activity. The combined use of green
tea with hydrochlorothiazide (HCTZ) showed myocardial protection by reducing the adverse effects of
HCTZ. This finding may be important for cancer patients with hypertension and ischemic heart
disease who can not undergo HCTZ monotherapy due to potential myocardial side effects. A study in
rats has shown that green tea reduces myocardial toxicity induced by cyclofosfamide in a dose
dependent manner. Adenosine is used for arrhythmia and green tea may inhibit the effects of
adenosine. Caffeine found in green tea reduces the sedative effect of benzodiazepines. Also, it can
increase blood pressure in patients using metoprolol and propanolol. Patients using warfarin should
not use green tea. Moderate quantities of K vitamin in green tea can reduce the effect of warfarin.
Green tea and aspirin should not be used together because they both increase the risk of bleeding by
acting as an inhibitor on the platelets that provide clotting. Laboratory tests involving the use of green
tea in combination with chemotherapeutics such as doxorubicin and tamoxifen have been shown to
improve the efficacy of these drugs. It has been shown that green and black tea extracts decreases the
sensitivity of prostate cancer cell to chemotherapy drugs. Because of this potential for interaction,
black and green tea (extracts of these teas) are not recommended especially for patients who receive
chemotherapy for prostate cancer. If clozapine is used less than 40 minutes after green tea ingestion,
the antipsychotic effect may reduce. If green tea is used in combination with ephedrine, it can increase
agitation, tremor, insomnia and weight loss. Green tea has been shown to reduce lithium blood levels.
The use of green tea with monoamine oxidase inhibitors such as phenelezine and tranylcypromine may
cause a hypertensive crisis. The combination of phenylpropanolamine and caffeine can result with
serious increase of blood pressure and mania. Clinical trials have showed that green tea and black tea

Corresponding Author: BURAK CEM SONER, burakcemsoner@gmail.com


1
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ AD.
2
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ AD.

1505
extracts reduces folic acid bioactivity even at low concentrations. Because of its phenolic content,
green tea can reduce the absorption of non-iron preparations. The inhibition of non-hem iron
absorption is a potential side effect. Two important flavonols of green tea (EG and EGCG) have an
important effect on organic anion-carrier polypeptides (OATP) that are expressed in enterocytes and
hepatocytes. ECG and EGCG inhibits uptake by OATP1A2, OATP1B1, and OATP2B1 in a
concentration-dependent way. However, OATP1B3-mediated uptake is strongly stimulated by EGCG.
Therefore, they can alter the pharmacokinetics of OATP substrates such as statins.

KEYWORDS
Camelia sinensis, green tea, drug interactions

1506
Poster Session 11
Submission ID: 1474

GARLIC AND ITS EFFECTS ON HEALTH

1 1 1 1
MERVE NUR GIZLICI , RUMEYSA KAMALAK , EZGI TOPTA BIYIKLI , ALI EMRAH BIYIKLI

ABSTRACT
GARLIC AND ITS EFFECTS ON HEALTH Garlic (Allium sativum) is used as flavoring

D
since very old ages. In some societies, it is seen the healing source. It is suggested that garlic has the
antioxidant, antibacterial, and antifungal effects and provides positive effects via sulfur and phenolic

TE
substances present in its content. The positive effects it shows on the health has made a current issue
to use it supplements beside the use of it nutrition and flavoring. A great majority of thiosulphates
(70%) the plant garlic has consist of allicin. This substance becomes active when garlic is mashed. It is
stated that allicin has a strong antibiotic and antifungal feature. In addition, it was demonstrated that it
was effective in gastrointestinal motility and regulation of the secretion of digestive enzymes. AIM: In
EN
this study, it was aimed to study the studies examining the effects of garlic on the health. METHOD:
Studying the studies examining the effects of garlic consumption on general health condition and some
chronic diseases by means of a comprehensive literature review, a compilation study was prepared in
the light of actual data. RESULTS: In the studies carried out, it was found that the substances such as
ES
diallyl sulfide, diallyl disulfide, 5-ethyl cysteine, n-acetyl cysteine present in garlic showed significant
antioxidant activity. In addition, it was seen that garlic consumption, increasing the effectivity of
microphages killing pathogens, was effective in strengthening immune system. In the studies carried
out on garlic and cardiovascular health, it was identified that garlic consumption reduced the level of
LDL cholesterol and increased the level of HDL cholesterol in individuals, and thanks to this, lowered
PR

the risk of cardiovascular disease. In the similar studies, it was suggested that garlic consumption,
reducing the formation of fibrin and plaque in the blood, could also significantly lower the risk of
heart attack. In the studies, examining the relationship between garlic consumption and blood pressure,
in the people having both high and low blood pressure, the positive effects of garlic consumption were
frequently identified. In the studies, examining the relationship between garlic consumption and Type
2 Diabetics, in addition to the diabetic medications, it was seen that garlic consumption exhibited
T

positive effects in the regulation of blood sugar. In the studies examining the relationship of garlic
with cancer, it was suggested that frequent garlic consumption may be protective against especially
O

breas, esophagus, gastric, colon, and rectum cancers and the studies on its use in cancer treatment are
limited. CONCLUSION:. Garlic, its antioxidative, antithrombotic, hypolipidemic, and hypoglycemic
N

effects, can be effective in reducing the morbidity and mortality of the disease. However, the actual
literature is evaluated, particularly about its quantity used as supplementary, there are no clear
suggestions and there is a need further studies. It should not be forgotten that higher dosages may
show negative effects for the hearth, liver, and renal functions.

KEYWORDS
garlic,nutrition,health,allicin

Corresponding Author: MERVE NUR GZLC, merve_ikbal@windowslive.com


1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEKOKULU/BESLENME VE DYETETK
BLM

1507
Poster Session 11
Submission ID: 1475

RESVERATROL AND CANCER

1 1 1 1
ELF BEYZA ELEB , HDA ZEYNEP KAYA , EZG TOPTA BIYIKLI , AL EMRAH BIYIKLI

ABSTRACT
RESVERATROL AND CANCER Cancer is a fatal disease showing increase tendency at the
present days. In this increase, unchangeable genetical factors as well as changeable environmental
factors play important role. It is stated that diet among the environmental factors has important effects.
In cancer process, on understanding of diet, the interest toward using some nutrients and supplements
has increased. This interest has also reflected to the literature. Resveratrol, which has a phytoalexin
structure and is synthesized as a response to stress factors in some plants, is one of the most studied
components with its cancer -protective feature. It is put forward that resveratrol shows its antioxidative
effect, removing free oxygen radicals from the environment. Resveratrol, present in higher rates in the
roots of plant, named Polygonum cupsidatum, grape, peanut, and ananas, is also used as supplement.
AIM:In this study, it was aimed to compile the actual studies examining the relationship between
resveratrol and cancer. METHOD:In the study, with examining the actual literature data, the protective
and therapeutic effect of resveratrol against cancer were evaluated in detail. RESULTS:In the studies,
in which the biological benefits and action mechanisms of resveratrol are examined, its
anticancerogenic and antioxidative effects were emphasized. It is considered that resveratrol shows its
anticarcinogenic effect by modulating enzymes making carcinogens ineffective. Depending on its
modulating these enzymes, it was seen that it could provide the positive effects on the metabolism of
some medications. Some studies suggest that resveratrol makes slower the tumor development,
inhibiting prostaglandin synthesis. In the studies, its antioxidative effects are examined, it was
emphasized that it effectively cleaned free oxygen radicals and other oxidative molecules and
prevented LDL oxidation. In the studies, in which the effect time of resveratrol was evaluated, due to
its rapidly metabolizing, that its time of being kept in the body is a short time like approx.. 1 hour was
remarkable. It was stated that this situation can impede to form a long term response. In the studies
examining the relationship between resveratrol intake and cancer formation, it is frequently seen that
there is a significant opposite relationship between resveratrol intake and cancer formation. In the
literature, rather than the use of resveratrol in the use of cancer treatment, its effects preventing cancer
were dealt with. Especially in preventing the formation of the colon, breast, and prostate cancer, it is
suggested that it is more effective. CONCLUSION:. There are the studies showing the effect of
resveratrol to prevent cancer, regression its formation step, and suppress it in the early period. The
dosage and tine of resveratrol used in these studies show a large variability according to the sorts of
cancer and there is no clear recommendations toward using it with the foods and supplements. For
being able to offer the suggestions about the consumption quantity and become clear its effectivity in
treatment, there is a need further studies. Keywords:Resveratrol, diet, cancer, , antioxidant

KEYWORDS
RESVERATROL,CANCER,DIET,ANTIOXIDANT

Corresponding Author: ELF BEYZA ELEB, beyzaelebi@gmail.com


1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEK OKULU BESLENME VE DYETETK

1508
Poster Session 11
Submission ID: 1476

GROWING AND HARVESTING OF ANATOLIAN SAGE (SALVIA


TRILOBA L.) ON BUROR TERRACES IN THE FORESTRY AREA.

1 1
MESUT TANDOAN , MEHMET ZDEMIR

ABSTRACT
Anatolian sage (Salvia triloba L.) which is medical and aromatic plant exported and collected
from forest areas is located in Marmara ireland, Turkey. In recent years, soil treatment during
afforestation practices are made in the form of grado (Buror Teras) with tracked or wheeled mini-
excavators in the fields having % 60 or more slope, in Turkey. In this study, Anatolia Sage (Salvia
triloba L.) seedlings produced in nursery conditions from seed Yalova originated is discussed
development and harvest of sage planted in the spring period of 2014 on Buror Terraces created in
the Kzlcaterzi Village, in the forest compartment number 261, in arky district, Tekirda province
of Marmara region. Sage seedlings in this area having 45-50 % slope planted in staggered with 40x40
cm. spacing and found soil tests positive reached to 40-45 cm heigh of annual sprout and harvested so
that the bottom 20 cm height. According to this result, it is possible to ensure success to grow
Anatolian sage as medical and aromatic plant on Buror terraces and in forested areas other forest tree
species or curved empty forest areas ecologically.

KEYWORDS
Anatolia Sage, Buror Terraces, Marmara, Cultivating

Corresponding Author: MESUT TANDOAN, mesuttandogan@ogm.gov.tr


1
TC ORMAN GENEL MDRL, MARMARA ORMANCILIK ARATIRMA ENSTTS MDRL

1509
Poster Session 11
Submission ID: 1477

THE USE OF PROBIOTIC AND PREBIOTIC IN OBESITY

1 1 1 1
TUBA ERDOAN , NEZIRE MERT , ALI EMRAH BIYIKLI , EZGI TOPTA BIYIKLI

ABSTRACT
Obesity is defined as excessive fat accumulation in the body due to the fact that the energy
taken in the body is more than the energy spent. It is a serious problem of public health, which shows
increase in the developed and the developing countries. Obesity is a process ranging with
inflammation in the body and brings together with it a number of complication such as hyperlipidemia,
hypertension, Type 2 diabetics, metabolic syndrome, fatty liver disease, coronary heart disease, and
the premature death. In the recent years, the view that in the development of obesity and its
complications, intestinal microbiota will also be effective revealed. Intestinal microbiota includes
different sorts of microorganism more than 500 and the number of these microorganisms is affected by
age, genetics, and environmental factors. The composition of intestinal mucosa of individual and
effect of bacteria here on energy transformation can form personal differences in weight loss and gain
of the individual. While Bacteriodetes decreases in the intestines of obese individuals, Firmicutes
increases, and also, the diversity of the other bacteria in intestines also decreases. There are some
studies showing that probiotics and prebiotics regulate intestinal flora. The resources of probiotics are
fermented milk products, pharmacologic products, prepared from the live cells of the probiotic
bacteria, and foods and drinks, enriched with probiotics. The resources of prebiotics, which form a
feeding place for probiotics, are the nutrients such as onion, garlic, wheat, leek, banana, escarole, earth
apple, artichoke, soya, dry legumes, asparagus, and tomato. AIM: In this study, it was aimed to
compile the studies examining the relationship between the use of probiotic and prebiotic and energy
balance in obesity. METHOD: In the study, actual literature studies were evaluated, which examines
the effect of the use of probiotic in obese people on both body weight and fat mass and complications
of obesity as a result of both variations of intestinal flora. RESULTS: The studies carried out showed
that intestinal bacteria had the different effects in the decomposition of energy from nutritional
elements and in its usability and storage. In especially obese people, the increasing Firmicutes filum
producing in high amount of short chained fatty acid from the fibers, causes more energy production
in the body. A number of studies were met, which show that the use of probiotic and prebiotic use in
obese people provides decreases in body mass index, thickness of skin curl, and serum glucose, In
similar way, there are also some studies showing that significant improvements were provided at the
level of insulin resistance, lipid, and adipokine, There are also studies showing that the use of prebiotic
provides increase in the feeling of saturity, weight loss, and decrease at the level of LDL cholesterol
level. In the studies examining the effect of prebiotic use on the complications of obesity, it was seen
that it showed positive effect on intestinal permeability , metabolic endotoxemia, adiposity, and insulin
resistance. CONCLUSION: In the treatment of obesity, in addition to the behavioral change, physical
activity, and diet treatment, the use of pro-prebiotics can be an alternative. For intestinal flora playing
role in energy balance, it is necessary for the use of pro-prebiotic to be important. The studies on

Corresponding Author: TUBA ERDOAN, dyttugba7@gmail.com


1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEKOKULU BESLENME VE DYETETK
BLM

1510
intake as supplement were dealt with rather than intake of nutrition resourced intake. The studies on
probiotic and prebiotic nutritional resources should be increased. For being able to offer clearer
suggestions about the sorts and quantities that are necessary to be used, there is a need further study.

KEYWORDS
obesity, probiotic, prebiotic, microbiota

1511
Poster Session 11
Submission ID: 1478

USE OF PHYTOTHERAPY IN SURGICAL NURSING

1 2
CEYDA UZUN AHN , DILEK LNGR

ABSTRACT
Today; -in spite of fast advancements in diagnosis, care and treatment in diseases- such factors
as peoples wish to have more control and responsibility over their treatments, to use relieving
interventions against symptoms, health care teams inability to spare enough time, cost of traditional
treatment modalities and psychologically feeling better have increased interest in complementary and
alternative treatments. In this sense, phytotherapy deriving Greek words phyton (herb) and
therapeia (therapy)- means curing and healing with herbs. Phytotherapy is considered as the practice
of pharmacognosis into modern treatments and is also called as phytopharmacy. Phytotherapy used
in the care and treatment of diseases, promotion and maintenance of health by all cultures since the
beginning of civilization- has used every kind of herbs as medication. For example; all of the
medication systems that include Native Americans healing methods, Indian Ayurveda system and
traditional Chinese medicine have placed herbs into their treatment modalities. Today; in the
developed countries, Turkish and Asian medicine; numerous individuals utilize herbal products alone
or mixed treatment products as a treatment modality in order to cure many diseases without consulting
any medical experts. When some of the herbal medicines often used are examined in relation to
surgical nursing; there may occur some side effects among patients. Use of some of these -like garlic,
gingko biloba, ginseng, echinacea and St. Johns wort surgical- should be quitted before surgeries. As
a result; although herms are frequently used for therapeutic purposes they may be omitted by health
care personnel. Therefore; nurses should be aware of effects, side effects, drug interactions (drugs that
should not be used together) of herbal medicines very well.

KEYWORDS
Phytotherapy, Surgical Nursing

Corresponding Author: CEYDA UZUN AHN, ceydam61@gmail.com


1
RECEP TAYYP ERDOAN NVERSTES SALIK HZMETLER MESLEK YKSEKOKULU
2
KARADENZ TEKNK NVERSTES SALIK BLMLER FAKLTES

1512
Poster Session 11
Submission ID: 1479

PHYTOESTROGENS EFFECTS ON MENOPAUSE SYMPTOMS

1 1 1 1 1 1
BRA GZ , GLIZER ELIK , MBERRA IL , EZGI TOPTA BIYIKLI , ALI EMRAH BIYIKLI , ELMAS ERSZ

ABSTRACT
Menopause period which is hormonal, physiological, psychological changes on women, starts
at 45-55 years old, after the end of menstruation. Estrogen hormone is the most effective factor during
menopause. Due to the decreasing estrogen level, the symptoms occurring after the menopause are
related with the estrogen. Therefore phytoestrogens are thought to be helpful to cope with these
symptoms. Phytoestrogens are used for estrogen replacement cure for menopause women.
Phytoestrogens structure and functions are similar like endogen estrogen 17- estradiol hence it makes
them to connect to estrogen receptors more easily. OBJECTIVE: In this review, the studies related
with phytoestrogens effects on menopause symptoms are investigated. METHOD: Phytoestrogens
nutrition and supplementation properties and their effects to the menopause symptoms are investigated
through literature. RESULTS: Use of phytoestrogens as nutrition and supplementation on healthy
women which are on menopause, shows positive results on the symptoms of menopause. When
phytoestrogens are used as nutrition for the women which are on premenopausal or postmenopausal
stage is helpful for constriction, sleeping pattern, anxiety, depression and irritability. Also,
phytoestrogens use as nutrition and supplementation have positive effects on vaginal dryness, libido,
hirsutism on facial area and dryness on the skin. On the other hand, use of phytoestrogens as
supplementation have positive effects on vasomotor menopause symptoms and life quality. On some
of studies made it is provided that, women who use phytoestrogens as supplementation, the
menopause symptoms are significantly decreased. CONCLUSION: Menopause symptoms have
negative effects on premenopausal and postmenopausal womens life quality. Phytoestrogen
supplementation could decrease menopause symptoms as an alternative for hormone replacement cure.
However, there should be more studies to be made for the amount of phytoestrogen consumption
during the menopause.

KEYWORDS
Phytoestrogens, nutrition, menopause

Corresponding Author: BRA GZ, gozbusra@gmail.com


1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEKOKULU

1513
Poster Session 11
Submission ID: 1480

SOME CHEMICAL AND SENSORY PROPERTIES OF GRISSINI


PREPARED WITH GARDEN CRESS (LEPIDIUM SATIVUM L.) SEED
POWDER

1 2 1 1
NKAN URGANCI , ALI GNC , FATMA IIK , LYAS ELIK

ABSTRACT
Yet, thanks to the inception and upcoming of the science of Foods and Nutrition, a number of
non- conventional food stuffs have been explored, analyzed, processed and used up in the
development of food products, all in a bid to end their oblivion on one hand and accruing benefit to the
masses on the other. Garden cress (Lepidium sativum L.) is such a food stuff that abounds not only in
nutrients but also in health enhancing phytochemicals. Garden cress is an annual erect herbaceous
plant, growing up to 30 cm. It belongs to Cruciferae (Brassica) family, widely grown in Europe, South
West Asia and US. The plant requires minimal agricultural resources, grows well in semi-arid regions
and low fertility soils. The seeds can be harvested in 7090 days and the yield is calculated to be 800
1000 kg/ha. It is a well known culinary herb and the leaves are widely used as garnish and are
consumed raw in salads. The plant is known to possess varied medicinal properties. Leaves of this
plant are diuretic and gently stimulant. The seeds are traditionally used in the diet of lactating woman
to induce milk secretion. The seeds are aperient, antibacterial, gastrointestinal stimulant, diuretic,
tonic, demulcent, aphrodisiac, carminative, galactagogue and emmenagogue. It is also reported that the
plant possess antihemogglutinating, hypoglycemic, antihypertensive, fracture healing properties and
significant bronchodilatory activities. Also a preliminary pharmacological study on seeds of garden
cress has suggested the presence of cardioactive substance and is shown to have probable action
through adrenergic mechanisms. Aqueous extract of garden cress was found to have antihypertensive
and diuretic effect when studied both in normotensive and spontaneously hypertensive rats. It is useful
in hiccup, dysentery, diarrhea and skin disease caused by impurities of blood as well. The oil of the
garden cress seeds is rich in alpha linolenic acid, and contains an ideal ratio of x-3 fatty acids (n-3) and
x-6 fatty acids (n-6). Recent studies have proved the preventive effect of x-3 polyunsaturated fatty
acids, especially alpha linolenic acid, on different types of cancer, including breast, in both animals or
cell line models, and in the treatment of cancer. Bakery products are the most consumed foods in the
world and among these products. Grissini are long, thin pieces of crispy, dry bread. They are better
known in many English speaking nations as breadstick or bread sticks, and they are a popular
accompaniment to many Italian meals. It is originated in Torino in Piemonte, a region of northwest
Italy. Grissini were actually invented around the end of the 17th century to cure the health problems of
young Duke Vittorio Amedeo II of Savoy. In this study, grissini were produced by the addition of 0.5,
1 and 2% of garden cress seed powder to grissini formulations, texture characteristics, color values,
some chemical properties such as oil and ash content and some sensory properties of grissini were
determined. It was found that the ash and oil contents of the grissini increased significantly (p<0.05)
by increasing the amount of garden crees seed powder in the formulation. In color analysis, it was seen

Corresponding Author: NKAN URGANCI, unkanurganci@hotmail.com


1
PAMUKKALE NVERSTES
2
ADNAN MENDERES NVERSTES

1514
that increasing the addition rate caused decreases in Hunter L value, and increases in a value. In the
sensory evaluation, the panelists gave scores for crust color, hardness, smell, chewiness, flavor, and
overall acceptance. Grissini having 1% and 2 % of garden cress received the best liking scores
(P<0.05) in color. The samples having 0,5% of garden cress seed powder recieved lower score than
other samples, altough they have been received sensory scores higher than 3.5, which was the
midpoint of hedonic scale.

KEYWORDS
grissini, Garden cress, seed, nutrition

1515
Poster Session 11
Submission ID: 1481

DETERMINATION OF SOME PHYSICAL, TEXTURAL AND


SENSORY PROPERTIES OF CRACKERS PRODUCED BY ADDITION
OF ZAHTER (THYMBRA SPICATA)

1 2 2 2
ALI GNC , NKAN URGANCI , LYAS ELIK , FATMA IIK

ABSTRACT
Plants and spices are generally used to bring aroma and colour in foods. In addition, they
provide antioxidant and antimicrobial properties to the products. They also have therapeutic and / or
preventive effects for many disorders, especially heart, vascular, diabetic and cancer diseases. With
these features they are extremely important in human diet. Zahter (Thymbra spicata), also known as
"Karaba kekii", "Karakekik" or "Da kekii" in Turkish is a plant specie belonging to the family of
Lamiaceae. It is a plant that has pink flowers and it has a height of about half a meter. It includes
essential oils and tannins. It can be grown almost everywhere in our country with the consumption is
mostly in Gaziantep and Hatay. It is consumed with its unique flavor in meals, in teas, in salads, in
olive oil and with different spices at breakfast or raw. In this study, crackers were produced by
substituting 1%, 2% and 4% of milled zahter instead of wheat flour. Moisture, ash, colour values and
textural and sensory qualities of the crackers were determined. It has been determined that by
increasing the zahter rates in the formulation, the amount of ash increased and the hardness decreased.
In sensory analysis, panelists evaluated cracker samples with respect to external colour, odor,
hardness, flavor, chewiness and overall acceptance. In color analysis, Hunter L and a values were
decreased and b values were increased by increasing zahter ratios. In sensory evaluation, crackers
produced with 2% of zahter received best liking scores in overall acceptance.

KEYWORDS
Cracker, Zahter, Thyme.

Corresponding Author: AL GNC, ali.goncu@adu.edu.tr


1
ADNAN MENDERES NVERSTES, NE MESLEK YKSEKOKULU, GIDA LEME BLM, AYDIN.
2
PAMUKKAKE NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM, DENZL.

1516
Poster Session 11
Submission ID: 1482

DETERMINATION OF SOME QUALITY CHARACTERISTICS OF


MUFFIN PREPARED WITH RIPENED HEMP SEED (CANNABIS
SATIVA L.)

1 2 1 1
NKAN URGANCI , ALI GNC , FATMA IIK , LYAS ELIK

ABSTRACT
Industrial hemp (Cannabis sativa L.) has been an important source of food, fiber and folk
medicinal preparations for thousands of years in the Old World. Both the ripened seed of hemp and
seed meal are excellent sources of dietary oil, fiber and protein. It contains 2025% protein; the
storage proteins consist mainly of edestin (globulin) and albumin, with a superior essential amino acid
profile and high digestibility. Is also has 2030% carbohydrates, 2535% oil (composed primarily of
linoleic and a-linolenic acids) and 1015% insoluble fibre and a rich array of minerals. The hemp
seed, in addition to its nutritional value, has demonstrated positive health benefits, including the
lowering of cholesterol and high blood pressure. The increased utilization of hemp seed for edible oil
production has caused abundant amounts of protein-rich meal, which serves as a suitable raw material
for production of peptide products, because of the presence of high levels of residues, especially
arginine and branched chain amino acids, which are desirable components of bioactive peptides.
Studies of hemp seed oils are expected to follow and may be extended to include clinical studies, such
as arthritis, hypertension, diabetes, cancer, gastrointestinal disorders, ulcers, chronic fatigue syndrome,
lupus, and more. Bakery products are the most consumed foods in the world and among these
products; cakes are the most popular products due to their deliciousness and special organoleptic
characteristics. Among different types of cakes, Muffin cake has a special position due to its
deliciousness. In this study, muffin cakes were produced by the addition of 2, 4 and 6% of hemp seeds
to cake formulations and color values, some chemical and sensory properties of cakes were
determined. It was found that the ash and oil contents of muffin cakes increased by increasing the
amount hemp seeds in the formulation (P<0.05). In color analyses, it was seen that increasing the
addition rate caused decreases significantly (p<0.05) in the all Hunter L,a,b values in crumb color. In
the sensory evaluation, the panelists gave scores for crust color, crumb color, crumb cell structure,
texture, smell, chewiness, flavor, and overall acceptance. The samples having 2% of hemp seeds
recieved best liking score on overall acceptance. All the muffin samples recieved sensory scores more
than 3.5 which was the midpoint of hedonic scale.

KEYWORDS
muffin, hemp seed, sensory

Corresponding Author: NKAN URGANCI, unkanurganci@hotmail.com


1
PAMUKKALE NVERSTES
2
ADNAN MENDERES NVERSTES

1517
Poster Session 11
Submission ID: 1483

UTILIZATION OF BUCKWHEAT (FAGOPYRUM) IN CEREAL


TECHNOLOGY

1 2 2 2
ALI GNC , NKAN URGANCI , LYAS ELIK , FATMA IIK

ABSTRACT
Buckwheat is a plant species of the genus Fagopyrum from the family Polygonaceae. Unlike
the important grains such as wheat, corn, oats, rice and barley, it can be adapted to develop in a short
time at high altitudes (0-4200 m). Buckwheat is one of the most important alternative crops that can be
grown without using artificial fertilizers, pesticides and other chemicals. It is a one-year-old plant that
can grow even in cool climates and in poor soils. Although it is in the Pseudocereal class, it is mostly
classified in cereals. Because its chemical composition and utilization areas are similar with cereals.
Nine varieties of buckwheat can be cultivated, but two varieties of them are cultivated mostly. The
buckwheat species most commonly used in bakery products is Fagopyrum esculentum moench.
Buckwheat seeds are in the form of a triangular pyramid. Seed hulls are bright or matt and brown,
black or gray in colour. It has been reported that it can be cultivated everywhere in our country and
government pays financial incentive for its cultivation. Unlike wheat, buckwheat does not contain
gluten. Buckwheat flour is commonly used with other gluten-free cereal flours and starches in the
world for the development of new dietary products for celiac disease. Buckwheat is an important
source for gluten-free products with its unique taste and aroma. The aim of this review is to give some
information about buckwheat using in breads, pasta, noodles, biscuits, cakes, tarhana, cookies, chips
and wafers.

KEYWORDS
Buckwheat, cereal, bakery products.

Corresponding Author: AL GNC, ali.goncu@adu.edu.tr


1
ADNAN MENDERES NVERSTES, NE MESLEK YKSEKOKULU, GIDA LEME BLM, AYDIN.
2
PAMUKKAKE NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM, DENZL.

1518
Poster Session 11
Submission ID: 1484

THE EFFECTS OF GNKGO BLOBA ON ALZHEIMER DISEASE

1 1 1 1 1
GZDE ALIKAN , GLSEREN AVCI , AYE LER , EZGI TOPTA BIYIKLI , ALI EMRAH BIYIKLI

ABSTRACT
Ginkgo biloba, is a 30-40 m high tree, which takes place in the class of ginkgoales, and can
live more than thousand years. It is a sort of the oldest tree still living on the earth and it is accepted as
a living fossil. Besides that it naturally grows in North China and Japan, it is also grown as
decorative plant in mild regions and parks and gardens. In order to use it as medication, the extract of
ginkgo biloba was first developed in Germany in 1965 and was introduced with the name of
"EGb761". Later, it was registered in France (IPSEN, Paris) and started to be produced in series.
Today, the extract of Ginkgo biloba takes place among the supplements often used. The extract of
standardized ginkgo biloba foliage contain flavonoid glycosides ( quersatin, kaempferol,,
isorhamnetin, etc. ) in the rate of 24%; terpenoids in the rate of 6%; and organic acid in the rate of 5-
10%. Thanks to terpenes and flavonoids, accepted pharmacologic active components in the content of
its leaves, it is considered that it has effect on especially cerebral vascular. This effect of it became
widespread the use of ginkgo biloba in weak memory depending on age and dementia. AIM :In this
study, it was aimed to compile the studies examining the effect of using the extract of using ginkgo
biloba for Alzheimer disease on cognitive disorders METHOD: In this compilation study researching
the relationship of the use of ginkgo biloba and Alzheimer, the actual literature data was examined and
the effect of using the extract of ginkgo biloba on Alzheimer diseases were examined by utilizing the
secondary study data. RESULTS: It is put forward that the pharmacologically active component
EGb761, used in a number of study, are flavonoid and terpenoids. In a number of studies, EGb761,
thanks to flavonoids in the content, reduced free radicals preparing ground for neuronal damage. It is
also emphasized that the components of ginkgolide and flavonoid have the inflammation preventive
effect seen in Alzheimer pathology. Troubles in mitochondrial functions are also associated with the
pathological changes seen in Alzheimer and it is considered that EGb761 has a protective effect on
mitochondrion. Besides that there are some studies showing that administrating the extract of ginkgo
biloba to Alzheimer patients significantly has an effect in positive direction, there are also some
studies, where its effect is not observed at all. Provided that high dosage is not used, since it is not
possible to see the useful effects of ginkgo biloba, it is emphasized that it is necessary to use, instead
of the plant itself, its extract. Dosage effect ranges between 240-720 mg per day and it was not dealt
with a net quantity. CONCLUSION: Due to its antioxidant activity and antiapoptotic and
antiinflammatory effects, its protective effects against amyloidogenes and A regeneration, it was
considered that Ginkgo biloba, can be used in Alzheimer treatment but this claim was not scientifically
verified and/or the contradictory results about the subject were reached. For being able to be better
understood of the effect mechanism, there is a need more comprehensive studies.

Corresponding Author: GZDE ALIKAN, dyt_gozde@hotmail.com


1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEKOKULU BESLENME VE DYETETK
BLM

1519
KEYWORDS
Alzheimer, ginkgo biloba, dementia

1520
Poster Session 11
Submission ID: 1485

DETERMINATION OF ANTIMICROBIAL ACTIVITIES OF SAGE AND


LINDEN HERBAL TEAS WHICH ARE SOLD BY TEA BAG AND
UNPACKED

1 1 1
DUYGU ZEHR , HALIL BRAHIM KAYA , MER MEK

ABSTRACT
In this study, four sage and four linden herbal tea bags purchased from national markets and
four unpacked sage and four unpacked linden herbal teas purchased from herbalist were used. The
herbal tea bags and unpacked teas are first placed in cups and freshly boiled hot water is added.
Brewing time for herbal tea bags was determined as 3-5 minutes and samples were taken for
antimicrobial activity studies. Well diffusion method was used to determine antimicrobial activity.
Micrococcus luteus was used as the indicator bacteria. As a result of the analyzes, it was determined
that antimicrobial activity of unpacked sage herbal teas were higher than sage herbal tea bags and
antimicrobial activity of linden herbal tea bags were higher than unpacked linden herbal teas.

KEYWORDS
Herbal Tea Bag, Unpacked Herbal Tea, Antimicrobial.

Corresponding Author: HALL BRAHM KAYA, hikaya@pau.edu.tr


1
PAMUKKALE UNVERSTY

1521
Poster Session 11
Submission ID: 1486

SOME ORGANOLEPTIC PROPERTIES OF BISCUITS FROM ADDING


TO CAROB (CEROTONA SLQUA) POWDER

1 1 1
FATMANUR BYKSARA , AYDIN YAPAR , CANSU TOPKAYA

ABSTRACT
Carob (Cerotonia siliqua ), also called locust, is an oldest plant of the world. It belongs to the
family of Fabaceae and grows in many parts of the Mediterranean region. Carob pod characterized by
a high content of carbohydrate , appreciable amount of protein, low levels of fat. Apart from
carbohydrates, high amounts of dietary fibre and polyphenols are characteristic of this Mediterranean
food. Dietary fibre itself or a diet rich in dietary fibre is known to exert a variety of physiological
effects, including improved digestion and attenuation of blood cholesterol and glucose levels.
Moreover, raw carob pods and carob pod flour contain substantial amounts of polyphenols especially
condensed tanin. Polyphenols exhibit a wide range of biological properties, and among these, the
antioxidant activity is the best known. Also, carob pods are rich in K, Ca and Mg. Pods of the carob
fruit have long been used as a raw material for food additives production. Due to its sweetness and
flavor similar to chocolate, as well as its low price less carob pods milled into flour are widely used in
the Mediterranean region as cocoa substitute for sweets, biscuits, and processed drinks production.
Additionally, the advantage of using carob powder as a cocoa substitute is that it does not contain
caffeine and theobromin. Biscuits are one of the most popularly consumed bakery products by people
all over the world. Traditional bakery products are produced from suitable materials, following the
classic procedure and using the proper equipment. The most common materials are flour, water, yeast,
sugar, vegetable fats or oils, eggs, starch, milk or milk products etc. Some of the reasons for such wide
popularity are their ready to eat nature, affordable cost, good nutritional quality, different varieties,
availability in different tastes and longer shelf life. Most of bakery products are used as a source for
incorporation of different nutritionally rich ingredients for their diversification. The aim of this study
was to determine the effect of carob powder on some sensory properties of biscuits and produce a
functional food by adding carob powder to the biscuits formulation. Biscuits samples were prepared
by using 4%, 6% and 8% of carob powder that were replaced with wheat flour. And also one party
was produced without carob powder as control. Color of biscuits samples were determined by
HunterlabMiniScan XE. L (lightness) and b (yellowness) value were decreased by increasing ratio of
carob powder whereas a (redness) value was incerased. Sensory parameters such as color, smell, taste,
crunchiness and overall acceptability were evaluated by a panel of 26 persons using 7-point hedonic
scale. All of the biscuits received sensory scores higher than 4.2 point. The biscuits having 6% carob
powder in the formulation were more liked than other samples having carob powder, in all criteria.

KEYWORDS
Biscuit, carob powder, functional food

Corresponding Author: FATMANUR BYKSARA, fatmanur_byksrc@hotmail.com


1
PAMUKKALE UNVERSTY

1522
Poster Session 11
Submission ID: 1487

IN VITRO CLONAL PROPAGATION OF A MEDICINALLY


IMPORTANT SPECIES: MUSCARI NEGLECTUM GUSS. EX. TEN

1 2 2
IDEM ALEV ZEL , DENIZ YZBAIOLU , FATMA NAL

ABSTRACT
Muscari neglectum Guss. Ex. Ten is both a vegetable and an ornamental, herbaceous perennial
plant that grows in the Mediterranean region with attractive and scented blue colored flowers. It is rich
in total phenolics, flavonoids, monomeric, and anthocyanin contents with high antioxidant and
antimicrobial activity. In this species, germination, propagation and seed formation is difficult due to
high seed dormancy. The aim of this study was to develop a protocol for rapid and efficient
multiplication of this plant which is rich in medicinally important metabolites under in vitro
conditions. Mass propagation of surface sterilized M. neglectum bulbs was achieved using 4 weeks
cold stored bulbs by vertically slicing them in to four. Each quarter of the bulblets was used to obtain
twin bulb scales. These were cultured on MS medium containing different concentrations of
BAP+NAA. Single bulblet bud noted on MS medium without plant growth regulators used as control.
Mean number of bulb buds per explant excluding control ranged 1.5-17.50. The bulblet regeneration
percentage ranged from 33.33 to 100%. MS medium containing 8.88 M BAP-10.74 M NAA and
17.76 M BAP-10.74 M NAA induced 100% regeneration. Mean number of bulblets per explant
ranged from 1.25 to 8.00 per explant with bulblet diameter of 0.10 to 0.30 cm. These bulblets grew in
the form of laminal out growths without callusing. Maximum number of bulblets per explants was
noted on MS medium containing 17.76 M BAP+2.685 M NAA. These bulblets had mean bulblet
diameter of 0.25 - 0.30 cm. These bulblets post subculturing for eight weeks, increased their diameter
in range of 0.31 to 0.48 cm. MS medium containing 8.88M BAP+10.74M NAA showed maximum
increase in the bulblet diameter. Variable axillary regeneration in range of 25-100% was also noted on
each concentration of plant growth regulators post sub culturing. Mean number of axillary bulblets on
the mother explants ranged 1-3.04 with bulblet diameter of 0.12 to 0.30 cm. The largest axillary
bulblets were noted on MS medium containing 8.88 M BAP-10.74 M NAA. Following 3-4 weeks,
these bulblets induced spontaneous root initials variably. These developed into visible roots at the end
of 8th week. In vitro cultured plants did not differ from normal plants growing in the wild and were
uniform in their morphologic characteristics. A novel protocol for micropropagation of this plant has
been established. It can be concluded that in vitro production of M. neglectum bulbs with significant
medicinal value is more advantageous, compared to its propagation under wild conditions; where it
takes 4 -5 years to grow to the desired size.

KEYWORDS
Muscari neglectum, in vitro, clonal propagation, regeneration, rooting, acclimatization

Corresponding Author: DEM ALEV ZEL, cigdemozel@gmail.com


1
1DEPARTMENT OF BOLOGY EDUCATON, FACULTY OF GAZ EDUCATON, GAZ UNVERSTY
2
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, GAZ UNVERSTY

1523
Poster Session 11
Submission ID: 1489

ANTIBACTERIAL ACTIVITIES OF THE ESSENTIAL OIL OF


THYMUS SPP., LAVANDULA SPP. AND SALVIA SPP.

1 1 1
CEREN YAVUZ , SEVGI MARAKLI , TUBA YILDIRIM

ABSTRACT
Many naturally occurring essential oils from medicinal plants and herbs have been shown to
possess antimicrobial properties and could serve as antimicrobial agents against pathogens [1]. Herbs
and their essential oils are the most efficient natural antimicrobial agents have long been used to
traditional drug [2]. The objective of this study was to investigate the antibacterial activity of essential
oils found in Thymus spp., Lavandula spp., Salvia spp. The essential oils of the these plants were
produced by the Clevenger hydrodistillation method. Plant materials (50 g) with small pieces placed in
a distillation apparatus with double distilled water and hydrodistilled for 3 h [3]. The antibacterial
activities of plant essential oils were determined by disc diffusion method and were tested against
standard strains of Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Klebsiella
pneumoniae ATCC 70603, Pseudomonas aeruginosa ATCC 27853, Salmonella enteritidis ATCC
13076 and Proteus vulgaris ATCC 13315. Experiments were performed at three times. Consequently,
some of the essential oil investigated in this study showed varying levels of antibacterial effects
against the six bacteria. According to the results of disk diffusion method, the highest antibacterial
effect was identified essential oil of Thymus spp., Lavandula spp. and Salvia spp., respectively. To
evaluate the antibacterial activity of the all essential oil against the test bacteria, the essential oil of
Thymus spp. was effective on all test microorganisms. Antibacterial activity of the essential oil of
Salvia spp. against Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia and Pseudomonas
aeruginosa was found to be less effective than Salmonella enteritidis and Proteus vulgaris. Klebsiella
pneumonia and Pseudomonas aeruginosa was the most resistant strains in the test bacteria.

KEYWORDS
Essential oil, disc diffusion, antibacterial activities

Corresponding Author: CEREN YAVUZ, ceren.yavuz@amasya.edu.tr


1
AMASYA NVERSTES

1524
Poster Session 11
Submission ID: 1490

PLANTS FROM ASTERACEAE AND LAMIACEAE USED


TRADITIONALLY AGAINST GASTROINTESTINAL DISEASES IN
TURKEY

1 1 1 1
MELTEM GLE , SEIL YAZICI TTNI , HAKAN BARIAIK , NUR TAN

ABSTRACT
Turkey is rich in medicinal plants and still need more research to explore its treasure.
Medicinal plants and natural products are widely used by indigenous people. Historically developed
ethnobotanical heritage should be protected for new generations and further studies. As known there
are a large variety of plants used to manage different gastrointestinal problems; such as ulcers,
gastritis, reflux, indigestion, constipation, diarrhea, nausea, vomiting, hemorrhoids, intestinal parasites,
loss of appetite, halitosis; in Turkey. This study aims to report the herbs of most used Families
(Asteracea and Lamiaceae) among Turkish folk to treat gastrointestinal problems. Related studies were
reviewed. Consequently 24 plants belonging to Asteraceae and 38 plants belonging to Lamiaceae have
been identified (1, 2, 3). Literature studies on these plants have been carried out. Totally usages of 39
plants were consistent with previous literature (4). Due to the lack of literatural studies the purported
medicinal usages of 15 plants cannot be confirmed. Usages of 8 plants are conflicting use of the
information in the literature (4). This study might be useful for the researches about these species. 1)
Sargn S.A., Akiek E., Selvi S., 2013. An ethnobotanical study of medicinal plants used by the local
people of Alaehir (Manisa) in Turkey. Journal of Ethnopharmacology 150, 860-874. 2) Tetik F.,
Civelek S., Cakiloglu U., 2013. Traditional uses of some medicinal plants in Malatya (Turkey).
Journal of Ethnopharmacology 146, 331-346. 3) Bulut G., Tuzlac E., 2009. Folk medicinal plants of
Bayrami (anakkale-Turkey). Marmara Pharmaceutical Journal 19, 268-282. 4) Baytop T., 1984.
Trkiyede Bitkiler le Tedavi, stanbul niversitesi Yaynlar, stanbul

KEYWORDS
Asteraceae, Lamiaceae, Gastrointestinal, Ethnobotany

Corresponding Author: MELTEM GLE, meltemgulec@gmail.com


1
DEPARTMENT OF PHARMACOGNOSY, FACULTY OF PHARMACY, ISTANBUL UNVERSTY, 34116 ISTANBUL

1525
Poster Session 11
Submission ID: 1491

SUGAR COMPOSITION IN THE ACHENES OF THREE MEDICINAL


MATRICARIA L. (ASTERACEAE) TAXA

1 1 1 2
KEMAL VEHBI MAMOLU , HSEYIN NCEER , SEMA HAYIRLIOLU-AYAZ , MELAHAT ZCAN , FAIK AHMET
1
AYAZ

ABSTRACT
Matricaria L. is a genus of the tribe Anthemideae of the famiy Asteraceae and it is mostly
distributed in Europe, northern Africa, Macaronesia, western, south-western and central Asia, and
western North America. This widespread distribution and the diversity of habitats, such as vacant lots,
disturbed meadows, areas along roads and railroads, and waste and dry areas, in which Matricaria
occurs may result in different adaptations to diverse environments. Soluble sugars vary in tissues and
organs within and among taxa in the plant kingdom. They are essential to the maintenance of plant-
animals interactions. In this respect, carbohydrate reserves in leaves, fruits and seeds etc. are potential
food sources for organisms. In this study, we have investigated sugar composition in the fruits (achene
or cypsela) of three Matricaria taxa (Matricaria aurea, M. chamomilla var. chamomilla and M.
chamomilla var. recutita) for the first time in the literature. Soluble sugars in the achene samples (0.5
g) were separated and quantified. Fructose, glucose, sucrose and maltose were identified on
chromatograms. Matricaria chamomilla L. var. recutita exhibited a higher fructose (1.390.12) and
glucose (1.440.12) content, while M. aurea had lower fructose (0.330.02) and glucose (0.460.05)
content. Matricaria aurea exhibited higher sucrose (5.070.89) content, while M. chamomilla var.
recutita had lower sucrose (2.910.73) content. Matricaria aurea exhibited higher maltose (0.660.09)
content, while M. chamomilla var. chamomilla had lower maltose (0.340.06) content. This research
was supported by the Scientific and Technological Research Council of Turkey (TUBITAK Project
No. 106T162).

KEYWORDS
Matricaria, sugar, achene

Corresponding Author: KEMAL VEHB MAMOLU, kvimamoglu@ktu.edu.tr


1
KARADENZ TECHNCAL UNVERSTY, FACULTY OF SCENCES, DEPARTMENT OF BOLOGY, 61080
TRABZON
2
ARTVN ORUH UNVERSTY, FACULTY OF FORESTRY DEPARTMENT OF FOREST ENGNEERNG, ARTVN

1526
Poster Session 11
Submission ID: 1492

PHARMACOPOEIA AND MONOGRAPH ANALYSIS OF CYNARA


SCOLYMUS (ARTICHOKE)

1 1
SEVDE ESRA SERT , DUYGU KAYA BILECENOLU

ABSTRACT
Herbal monographs are guides that contain comprehensive information on the common names
of plants used for medicinal treatment, their botanical properties, chemical compositions, geographical
distributions, clinical characteristics, pharmacological properties, pharmacokinetic properties,
indications, contraindications, safe use data, clinical research data, etc.. There are many sources that
record the efficacy, quality criteria and safety of medicinal plants. Herbal medicines also have to be
manufactured according to the principles of good manufacturing practices (GMP), as well as modern
medicine. If the drug containing the correct characteristics of the correct plant source is supplied and
used, it can be ensured that the effect of the produced drug is always the same. This is possible with
standardization. The standards of pharmaceutical raw materials and auxiliary substances are registered
in pharmacopoeias. Pharmacopoeia is the official book that defines the most accurate raw materials by
the monographs which are constantly renewed by the specialist staff and that the pharmaceutical
industry is obliged to comply with in the countries where it is valid. Cynara scolymus L. (Artichoke) is
a plant species that is mostly grown in the eastern Mediterranean region of Turkey and consumed as
food. It is used as an appetizer, urine and bile extractor in infusion (2-3 %) for medicinal purposes.
Flowers of the plant carries no toxic compounds and thus are edible, while extracts prepared from
fresh leaves are frequently used in liver diseases. Artichoke is also known today as an aphrodisiac.
Artichoke has records in pharmacopoeia and monographs such as the worldly accepted European
Pharmacopoeia, Martindale, ESCOP Monographs, Commission E monographs, French
Pharmacopoeia. In this study, the features of the artichoke will be presented as appeared in the existing
monographs.

KEYWORDS
Cynara scolymus, artichoke, pharmacopoeia, monograph

Corresponding Author: SEVDE ESRA SERT, dyt.sevdeesrasert@gmail.com


1
ADNAN MENDERES NVERSTES, SALIK BLMLER FAKLTES, BESLENME VE DYETETK BLM,
AYDIN

1527
Poster Session 12
Submission ID: 1493

THE EFFECTS OF OREGANO ESSENTIAL OIL SUPPLEMENTATION


TO LAMB RATIONS ON SOME MICROBIOLOGICAL AND
PHYSIOCHEMICAL CHARACTERISTIC OF LAMB MEAT

1 2 2 3 4
HALIT MIK , SEVDA URAR GELEN , MERYEM AYDEMIR ATASEVER , RECEP GM , HAYRUNNISA ZL

ABSTRACT
Although oregano essential oil has common use in the cosmetics, pharmaceutical and food
sectors, information available on its effects on meat quality and shelf life, when incorporated into lamb
feed rations, is scarce. This study, the effects of oregano essential oil (OEO) in feed on the water
activity, pH, lipid peroxidation, colour parameters and microbial counts of the Musculus longissimus
dorsi (MLD) were investigated. The control group was fed on a basal ration and the OEO1 and OEO2
groups were fed on a basal ration added 200 mg/kg and 400 mg/kg oregano essential oil, respectively.
Akkaraman lambs were fed on experimental rations for a period of 56 days and were slaughtered at the
end of the fattening period. Meat quality parameters were investigated in the longissimus dorsi muscle
of the slaughtered lambs. Lipid peroxidation of the MLD was significantly affected by storage time
(ST), group (G) and ST*G, whilst pH was affected by ST. Lightness (L*) was significantly affected by
ST, G and ST*G; redness (a*) by ST and G; and yellowness (b*) by ST and ST*G. The
Micrococcus/Staphylococcus, total mesophilic aerobic bacteria and total psychrophilic aerobic
bacteria counts were significantly affected by ST, G and ST*G; Pseudomonas spp.counts by ST; and
coliform bacterial counts by ST and ST*G. The antioxidant and antimicrobial effects of oregano
essential oil were confirmed.

KEYWORDS
Oregano essential oil, Antioxidant, Antimicrobial, Lamb, Meat quality

Corresponding Author: HAYRUNNSA ZL, hayrunnisa@atauni.edu.tr


1
ATATRK NVERSTES, VETERNER FAKLTES, HAYVAN BESLEME VE BESLENME HASTALIKLARI
ANABLM DALI
2
ATATRK NVERSTES, VETERNER FAKLTES, GIDA HJYEN VE TEKNOLOJS BLM
3
CUMHURYET NVERSTES, VETERNER FAKLTES, HAYVAN BESLEME VE BESLENME HASTALIKLARI
ANABLM DALI
4
ATATRK NVERSTES, SALIK BLMLER FAKLTES, BESLENME VE DYETETK BLM

1528
Poster Session 12
Submission ID: 1494

MEDICINAL PLANTS SOLD IN THE HERBAL MARKETS IN ARI

1
KADIRIYE URU PARLAK , ZKAN SEVM

ABSTRACT

D
As result of this research carried out to determine the plants which were used for medicinal
aims by local people and sold in herbalists in Ar, local names of 85 species belonging to totally 42
families and medicinal uses were detected. The biggest 5 families in terms of their number of taxon

TE
were found as Lamiaceae (12 taxa), Asteraceae (9 taxa), Rosaceae (8 taxa), Apiaceae (6 taxa) and
Poaceae (5 taxa), respectively. The most frequently used parts of the plants were found respectively as
leaf (18 taxa), fruit (13 taxa), whole flower and plant (9 taxa each), seed and root (9 taxa each).
Distribution of taxa to phytogeographical regions were as Euro-Siberian 11, Irano-Turanian 12,
EN
Mediterranean 6, and 56 taxa were found as multi-region or region-unknown. Ar local people
generally used medicinal plants for cough, asthma, bronchitis, and influenza, digestive disorders and
kidney stone and disorders. The most common use of plants was as tea.
ES
KEYWORDS
Agri, Medicinal plants, Ethnobotany, Herbal markets
PR
T
O
N

Corresponding Author: ZKAN SEVM, ozkansevimsydv@gmail.com


1
ARI BRAHM EEN NVERSTES

1529
Poster Session 12
Submission ID: 1495

STINGING NETTLE COMPOSITION AND EFFECTS ON DISEASES

1 1 1
EYMA TEKE , RECEP PALAMUTOLU , CEMAL KASNAK

ABSTRACT
Stinging nettle (Urticaceae) is a native plant that naturally grows throughout Anatolia. In
Turkey, the most seen species is Urtica dioica. Stinging nettle (Urtica spp.) grows in border of field,
road and forest naturally and has local names such as dzlaan, adalak, dalagan, srg is annual or
perennial herbaceous medicinal plant. Especially in the Black Sea Region, the plant is grows in a wide
area and very rich in terms of chemical constituents and has been used as herbal medicine, food,
fibber, colour agent and cosmetic for many centuries. Stinging nettle roots, body, leaves and seeds are
used for this purpose. It has an important place in terms of its benefits over health and its protective
role in certain diseases. Traditional therapy gains importance with antiinflammatory, antirheumatic,
antidiabetic, antiviral, antioxidant effects and acute diuretic, natriuretic, hypotensive, cardiovascular
effects etc. In this study, information will be given on the general characteristics of the stinging nettle
plant, the components it contains, the areas of use, health implications and harmful effects will be
compiled.

KEYWORDS
Urtica dioica, Stinging nettle, medicinal plant, health

Corresponding Author: RECEP PALAMUTOLU, rpalamutoglu@aku.edu.tr


1
AFYON KOCATEPE NVERSTES

1530
Poster Session 12
Submission ID: 1497

DETERMINATION OF ENVIRONMENTALLY SENSITIVE AREAS IN


SEMIARID FOREST AND AGRICULTURE LAND USES

1 1 1
EBRU GL , SABIT ERAHN , MELDA DLARSLAN

D
ABSTRACT
In recent years, land degradation and decrease in biological productivity have made it

TE
necessary to take measures for combating desertification. This study was carried out in a semi-arid
forest and agricultural area with a size of 1000 ha located in the Sarkaya region of Yaprakl district,
approximately 45 km north-east of ankr province. Environmentally sensitive areas (ESAs) in
forests (pine and oak) and agricultural lands have been determined using the Desertification Indicator
System for Mediterranean Europe (DIS4ME) system developed for the Mediterranean countries. Soil
EN
samples were taken at 632 predetermined sites (252 from agricultural areas, 122 from oak forests and
258 from pine forests) and a desertification survey form was filled at each sampling site. ESAs
sensitivity score types were calculated using the DIS4ME model system. Model calculated and field
observed desertification risk values were compared to evaluate successes of DIS4ME. Significantly
ES
high correlation coefficient (r =0.88) was found between observed and scored values calculated with
DIS4ME. The ESAs type sensitivity score surface maps were created for the entire study area and the
corresponding land uses by geostatistical methods. The DIS4ME-calculated ESA type sensitivity
scores values range from 1.18 to 1.61. The majority of the study area (597.29 ha) was categorized as
fragile, and 370.61 ha in critical sensitivity class. The agricultural areas are more vulnerable to
PR

desertification than oak and pine forests, respectively. Land use change from forest to agriculture
should be avoided to prevent further degradation of the study area.

KEYWORDS
T

Desertification, geostatistics, environmentally sensitive areas, semi-arid forests.


O
N

Corresponding Author: EBRU GL, ebru@karatekin.edu.tr


1
ANKIRI KARATEKN NVERSTES

1531
Poster Session 12
Submission ID: 1499

INVESTIGATION OF ANTIDEPRESSANT EFFECTS OF GENTIANA


OLIVIERI IN THE CHRONIC UNPREDICTABLE STRESS MODEL OF
DEPRESSION IN THE RAT

1 2 3 2
AHMET BERK , SMET YILMAZ , NURETTIN ABACIOLU , MERVE GKIN KARAASLAN , EBRU KUYUMCU
2 2
SAVAN , MUSTAFA BAHADIR KAYMAZ

ABSTRACT
PURPOSE: Flowering herbs of Gentiana olivieri known with a local name as Afat, is
traditionally been used in south-east Anatolia to combat some mental disorders including depression in
the different regions of Turkey. The aim of this study is to investigate the antidepressant effects of G.
olivieri ethanol extract and the possibility of synergistic interactions with imipramine in combinations
of different doses on chronic unpredictable stress (CUS) -induced depression. MATERAL AND
METOD: Male Sprague dawley rats (n=8) were subjected to an experimental setting of CUS. The
herbal extracts administered orally (1000 mg/kg, 500 mg/kg and 200 mg/kg) alone and in combination
of imipramine (10 mg /kg) for 3 weeks during the CUS model; imipramine at 10 mg/kg/day given
orally as a positive control. CUS-induced depression was examined by measuring serotonin,
noradrenaline, dopamine and monoamine oxidase A (MAO-A) levels in brain tissue, changes in total
body weight and serum corticosterone levels and the behavioral parameters of depression. RESULTS:
Higher dose (1000 mg/kg) of G. olivieri and imipramine produced beneficial effects on the stressed
rats by effectively improving CUS-induced low sucrose consumption, increasing brain serotonin and
noradrenaline levels, reducing serum corticosterone and brain MAO-A levels in rats. G. olivieri at
doses of 500 mg/kg also increased the brain serotonin levels on the stressed rats. There was no
significant difference in body weight among the treatment groups. Our results also showed, unlike the
positive control imipramine, G. olivieri did not affect the brain dopamine levels. The combination use
of G. olivieri with imipramine did not show synergistic interactions. CONCLUSION: These findings
demonstrate the antidepressant effects of G. olivieri within a CUS model of depression and the
mechanism of action might be attributed to its attenuating abnormalities in monoaminergic system
functions in brain and improvement in hypothalamicpituitaryadrenal axis, although underlying
mechanism still remains to be further elucidated.

KEYWORDS
Antidepressant, Gentiana olivieri, Rat.

Corresponding Author: SMET YILMAZ, ismetyilmaz44@hotmail.com


1
SALIK BLMLER NVERSTES ELAZI ETM VE ARATIRMA HASTANES
2
NN NVERSTES
3
YAKIN DOU NVERSTES

1532
Poster Session 12
Submission ID: 1500

FORMULATION AND EVALUATION OF A GEL CONTAINING


NATURAL INGREDIENTS FOR THE TREATMENT OF CELLULITE

1 2 1
EBRU ALTUNTA , BURCU ZKAN , GLGN YENER

ABSTRACT
Cellulite is a cosmetically unacceptable problem that most women experience at some point in
their lifetime. Cellulite, also called as edematous fibrosclerotic panniculopathy and local or gynoid
lipodystrophy is characterized by irregular relief alterations to the skin surface of the affected areas,
giving orange peel, cottage cheese, or mattress aspect. It is frequently found on the thighs and buttocks
of women. Approximately 8590 % of women over 20 years are believed to have some degree of
cellulite. On the one hand, it has been recently described as a physiological condition aimed to
maximize adipose retention in order to ensure adequate caloric availability during pregnancy and
lactation, but cellulite is also a complex problem, involving several different factors and mechanisms,
such as metabolic imbalances, alterations in connective tissue structure, genetic factors, inflammatory
conditions, reduced microcirculation and hormonal factors. Regardless of the number of women
affected by cellulite, only a limited number of studies have been published in the scientifi c literature
so far, botanical derivatives being one of the main groups of evaluated compounds for its treatment. A
multi-target/multi-component strategy has been recently recognized as one of the best approaches to
counteract the main cellulitic symptoms and signs The objective of this study was to develop a stable
gel type formulation including a variety of natural ingredients (Centella asiatica extract, Coleus
forskohlii extract, Aesculus hippocastanum extract, Paullinia cupana oil, caffeine, L-Carnitine, alpha
tocopherol) which can be considered as a cosmetic product and evaluate its anti-cellulite efficacy and
safety against placebo with non-invasive biophysical techniques (Skin-ph-meter 900, Corneometer
CM 825, Aramo TS skin diagnosis system, Laser Doppler flowmeter, Infrared thermometer), clinical
assessments, a dermatological test and a subjective questionnaire over a period of four weeks. In this
content, twenty female volunteers (ages: 1865 yrs), affected by fat accumulations and/or slight-to-
moderate edematous-fibrosclerotic panniculopathy in the lower limbs were selected for the study. The
trial was conducted in a single-blind method with the comparison within subjects (each subject being
its own control), and volunteers were required to apply the test products on the thigh twice a day,
unilaterally, for a period of four consecutive weeks. They underwent two medical examinations, a
baseline evaluation at T0 before the beginning of the test and an evaluation at the end of the treatment
period T4.

KEYWORDS
Cellulite, herbal ingredients, topical gel, cosmetic product

Corresponding Author: EBRU ALTUNTA, ebru.altuntas@istanbul.edu.tr


1
STANBUL NVERSTES, ECZACILIK FAKLTES
2
YILDIZ TEKNK NVERSTES, BYOMHENDSLK BLM

1533
Poster Session 12
Submission ID: 1502

THE IMPORTANCE OF MEDICINAL AROMATIC PLANTS IN


PATIENTS ANAMNESIS IN TERMS OF DENTISTRY

1 2
OLGUN TOPAL , BURCU GYETMEZ TOPAL

ABSTRACT
The use of plants for medical treatment was started with the history of mankind, but
nowadays, with the desire for returning into the nature, its use have become widespread. Due to the
fact that these plants are natural, there is a strong belief that they have no harm. Since most patients
using medicinal aromatic plants do not know their side effects and their interactions with other
medicines; this information is ignored both by patients and dentists, while taking an anamnesis before
the dental procedure. In this study, it is aimed to emphasize the importance of the use of medicinal
aromatic plants by patients for various purposes in the anamnesis of dentistry, the possibility of
observing possible side effects and the importance of unconscious use of these medicinal plants.
Unconscious use of medicinal aromatic plants such as St. Johns Wort, garlic, ginger, ephedra, ginkgo,
etc. for various purposes may result in mild findings such as nausea and vomiting, as well as
cardiovascular, hematologic, allergic, psychiatric and neurological life threatening side effects. For the
dentists, especially in cases of hemorrhagic surgical interventions to be performed under local or
general anesthesia, this situation is very important and can lead to undesirable results during or after
the procedure. They also pose a risk for drug interactions. Consequently, it comes out that, lack of
communication and information between dentists and patients can lead to undesirable side effects
which may be vital during dental treatments. Therefore, when the patients' anamnesis is taken,
medicinal aromatic plants they use should be also questioned as well as the drugs they use. Dentists
should be informed about the potential side effects of medicinal plants commonly used for healing, the
public should be informed about this and the unconscious use of these plants should be avoided.

KEYWORDS
Medicinal aromatic plants; Dentistry; Side effect

Corresponding Author: OLGUN TOPAL, dt.olguntopal@gmail.com


1
ISPARTA AIZ VE D SALII MERKEZ (AIZ,D VE ENE CERRAHS UZMANI)
2
SERBEST (UZMAN OCUK D HEKM)

1534
Poster Session 12
Submission ID: 1503

USE OF ESSENTIAL OILS IN SEAFOOD PRESERVATION

1 1 1 2
AYE ZYILMAZ , SEVIL DEMRC , KRAN AKIR ARICA , ABDULLAH KSZ , DILAT BOZDOAN
3
KONUKAN

ABSTRACT
Essential oil is one of the many occuring compounds naturally founds in plants and find their
application in pharmaceutical, sanitary, cosmetic, agricultural, and food industries. There is an
increasing interest in natural products, intended not only to prevent some diseases, but also to prevent
the deterioration of fats and other constituents of foodstuffs. Essential oils can be identified as natural
food additives which can find useful application in food preservation. Some scientific studies either
vitro or vivo have clearly shown that essential oils have some components such as carvacrol, p-
cymene, and c-terpinene which provide natural preservation effects in food. Among the essential oils,
for example thyme and oregano essential oil, either by itself or with other combinations, oil has been
tested for antimicrobial, antifungal, and antioxidant effects for different types of seafood such as,
anchovy, red porgy, gilthead sea bream, ready-to-eat squid rings, Mediterranean octopus, sea bream
and Asian bass fish. Based on the data of all those previously reporting, results were found out to be
very promising.

KEYWORDS
seafood, essential oil, carvacrol, p-cymene, c-terpinene

Corresponding Author: AYE ZYILMAZ, aylaayse@gmail.com


1
ISKENDERUN TEKNK NVERSTES, DENZ BLMLER VE TEKNOLOJS FAKLTES, SKENDERUN-
HATAY, TRKYE
2
NECMETTN ERBAKAN NVERSTES,,BESLENME VE DYETETK KONYA, TRKYE
3
MUSTAFA KEMAL NVERSTES, ZRAAT FAKLTES, GIDA BLMLER ,HATAY, TRKYE

1535
Poster Session 12
Submission ID: 1506

ASSESSING THE ANTIDEPRESSANT EFFECTS OF GENTIANA


OLIVIERI IN THE FORCED SWIM TEST IN RATS

1 2 3 2
AHMET BERK , SMET YILMAZ , NURETTIN ABACIOLU , MERVE GKIN KARAASLAN , EBRU KUYUMCU
2 2
SAVAN , MUSTAFA BAHADIR KAYMAZ

ABSTRACT
PURPOSE: The aim of this study was to investigate whether Gentiana olivieri herbal extrats
in different doses exert antidepressant effects, and the possibility of synergistic interactions with
imipramine in combinations of different doses in experimental animal models: the forced swimming
test (FST), and the open field test. MATERAL AND METOD: Male Sprague dawley rats were
administered the ethanol extract of G. olivieri orally (1000 mg/kg, 500 mg/kg and 200 mg/kg) alone
and in combination of imipramine (10 mg /kg) for 7 days before the FST. Imipramine was used as
positive control in this study and the behavioral parameters were examined. RESULTS: The results
showed that orally administration of the imipramine (10 mg/kg), ethanol extract of G. olivieri (200,
500 and 1000mg/kg) alone and in combinations of imipramine (10 mg/kg) significantly reduced the
duration of immobility in FST, which was not due to the alteration in the locomotor activity. G.
olivieri did not show any synergistic interactions with imipramine. CONCLUSION: This study
suggests some evidence of antidepressant therapeutic potential of G. olivieri.

KEYWORDS
Forced swim test, Gentiana olivieri, Rat.

Corresponding Author: AHMET BERK, ahmet.berk@saglik.gov.tr


1
SALIK BLMLER NVERSTES ELAZI ETM VE ARATIRMA HASTANES
2
NN NVERSTES
3
YAKIN DOU NVERSTES

1536
Poster Session 12
Submission ID: 1507

THE ESSENTIAL OIL COMPOSITON OF HYPERICUM LYDIUM AND


H. HYSSOPIFOLIUM VAR. ELONGATUM

1 2 3 4 5
MEHMET AKDENIZ , ABDULSELAM ERTA , IIL AYDIN , MEHMET FIRAT , FIRAT AYDIN

ABSTRACT
The genus Hypericum a member of Hypericaceae family, is represented by 100 taxa, 45 being
endemic to Turkey. In Turkish folk medicine, the genus Hypericum is known as sar kantaron,
kantaron, binbirdelik otu, mayasl otu and most of them, especially H. perforatum, have been used for
the treatment of burns, wounds, hemorroids, diarrhea and ulcers. Aqueous extracts of the flowering
aerial parts of the Hypericum species are used in the treatment of neuralgia, anxiety, neurosis and
depression. The essential oil compositions of about 50 different Hypericum species have been
identified in the previous studies. In this study, the essential oil contents of H. lydium and H.
hyssopifolium var. elongatum was analyzed by GC-MS/FID. The dried aerial parts of species were cut
into small pieces and subjected to hydro- distillation with water for 4 h, using a Clevenger-type
apparatus to produce essential oils which were dried over anhydrous sodium sulphate and stored at
by dichloromethane (1:3, v/v) before the GC run.
Identification of the compounds was based on the comparison of their retention times and mass spectra
with those obtained from authentic samples and/or the NIST and Wiley spectra as well as the literature
data. The major components of the essential oils were identified as pinen, caryophyllene, -
eudesmene, -silenene, -elemene and caryophyllene oxide for H. lydium and -ocimene, pinen,
pinen, caryophyllene and germacrene D for H. hyssopifolium var. elongatum. Acknowledgements:
This work was supported by Research Fund of the Dicle University FEN.15.012

KEYWORDS
H. Lydium, H. hyssopifolium var. elongatum, Essential Oil, GC-MS/FID

Corresponding Author: FIRAT AYDIN, faydin@dicle.edu.tr


1
DCLE UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF ANALYTCAL CHEMSTRY;DYARBAKR
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY,DYARBAKR
3
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY, DYARBAKR
4
YZNC YIL UNVERSTY, FACULTY OF EDUCATON, DEPARTMENT OF BOLOGY, VAN
5
DCLE UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF ANALYTCAL CHEMSTRY, DYARBAKR

1537
Poster Session 12
Submission ID: 1508

RESEARCH REGARDING WITH COOKING VEGETABLE OIL


CONSUMPTION HABITS OF STUDENTS

1 2 3
NURAN ERDEM , DIDEM NAY DERN , MEHTAP GRSOY

ABSTRACT
This research is a situation determination and it was planned and executed for determining
cooking vegetable oil consumption habits and frequencies of vocational higher education students. 360
students who attend 1. and 2. grades of Aksaray University Gzelyurt Vocational School form the
universe of this research. (Total number of students who are enrolled to school is 550). Random
sampling method was used in research and total 227 students consisting of 125 voluntary male student
and 102 female students participated to study (participation rate is 63.06%) Research data were
collected between October 2016- November 2016 by using questionnaire form. Questionnaire form
consists of several questions for determining cooking vegetable oil consumption frequencies and
habits of students and general information about students. SPSS package program was used for
evaluation of data and necessary statistical analysis was made. Ages of students change between 17
and 38 and average age is 19.591.97 years. Average body weight of students who had participated to
research is 63.2011.71 kg, their average height is 169.198.70 cm, average Body Mass Index (BMI)
is 21.993.08 kg/m2. It was determined that 61.7% of students mostly use sunflower seed oil. It was
observed that Majority of students (77.5%) spread butter over bread more than half of them (56.4%)
prefers food with normal oil. It was observed that Majority of students use sunflower seed oil (78.4%),
butter (60.8%), and olive oil (51.1%), the sometimes consume margarine (47.1%), tail fat (45.9%),
corn oil (%45.4), semi fluid margarine (65.2%), they never consume cotton seed oil (75.8%), canola
seed oil (68.3%), hazelnut oil (63.0%), lard (%56.4). Consumption of oils which are sources of energy
should not be avoided provided that they are balanced. Vegetable oils should be used alternately for
getting omega-3, omega-6 and omega-9 fatty acids and awareness of people should be increased.

KEYWORDS
Cooking vegetable oil, oil consumption, fatty acids, omega-3.

Corresponding Author: DDEM NAY DERN, donay@selcuk.edu.tr


1
AKSARAY NVERSTES, GZELYURT MESLEK YKSEKOKULU, GIDA LEME BLM, AKSARAY
2
SELUK NVERSTES, SALIK BLMLER FAKLTES, BESLENME VE DYETETK BLM, KONYA
3
AKSARAY NVERSTES, GZELYURT MYO, BTKSEL VE HAYVANSAL RETM BLM, BTK
KORUMA PROGRAMI, AKSARAY

1538
Poster Session 12
Submission ID: 1509

EVALUATION OF COLOR AND CHLOROPHYLL CONTENT OF


PARSLEY, DILL, CORIANDER AND CHERVIL LEAVES SUBJECTED
TO MICROWAVE AND NATURAL DRYING

1 1
LKNUR ALIBA , ASLIHAN YILMAZ

ABSTRACT
Parsley, dill, coriander and chervil leaves, which weigh 20 0.06 g with moisture of 83.70
0.06%, 86.96 0.10%, 82.05 0.02% and 77.99 0.01%, respectively, were subjected to microwave
and natural drying. Drying continued until the moisture of parsley, dill, coriander and chervil leaves
was decreased to 8.18 0.01%, 8.39 0.08%, 8.23 0.01% and 8.08 0.06%, respectively. Drying
trials in microwave drying were 900 and 400 W. Drying periods for parsley, dill, coriander and chervil
leaves lasted for 11-23, 16-26, 18-30 and 12-22 min , respectively, at microwave drying. Moreover,
the duration of natural drying trials for parsley, dill, coriander and chervil was 4.5, 3, 5.5 and 5 days,
respectively. Energy consumption of parsley, dill, coriander and chervil leaves during 400 W and 900
W at microwave drying were measured as 0.153 - 0.165, 0.173 0.240, 0.200 0.270 and 0.147
0.180 kWh, respectively. Measured values of moisture were compared with predicted values obtained
from five thin-layer equations. The best model was selected for each trial according to coefficient of
determination (R2) as well as standard error of estimated (SEE), root mean square error (RMSE) and
Chi-square (2). The highest R2 and the lowest SEE, RMSE and 2 were used as the deciding factor in
all thin layer equations. Hue angles of fresh parsley, dill, coriander and chervil leaves were 113.92,
107.73, 104,37 and 109.60, respectively. The best color parameters of parsley and dill leaves were
determined for microwave drying at 900 W, while the worst color parameters were obtained for
natural drying. Furthermore, the closest color parameters to fresh chervil were found in microwave
drying at 400 W, whereas the furthest color parameters were determined in natural drying. Also,
microwave drying at 900 W provided the best color parameters following fresh coriander, while
microwave drying at 400 W gave the worst color parameters of coriander leaves. The highest
chlorophyll concentration of parsley and chervil leaves was measured in microwave drying at 400 W
unlike dill and coriander leaves in microwave drying at 900 W. Consequently, for all products, not
only drying parameters such as drying period and energy consumption but also quality parameters
related to color and chlorophyll concentration were used in decisive factors in choosing the best drying
method.

KEYWORDS
Chlorophyll, color, chervil, coriander, dill, microwave drying, natural drying, parsley.

Corresponding Author: LKNUR ALBA, ialibas@uludag.edu.tr


1
ULUDA NVERSTES

1539
Poster Session 12
Submission ID: 1510

STANDARDIZATION OF EDUCATION PROGRAMS OF HERBAL


MEDICINE AND AROMATIC PLANTS TRAINING IN TURKEY

1
MER ARSLAN KALAFATCILAR

ABSTRACT
The aim of Herbal Medicine and Aromatic Plants Programs in universities of Turkey includes;
1. To prevent uncontrolled picking of plants and to prevent consumption of these special herbs and
plants. This can be achieved by culture of plants in appropriate situations and standardization of
procedures. 2. To provide hygienic circumstances and standardization of places where these herbs and
plants are being sold. 3. To train members who can work with medical doctors and pharmacists to
produce drugs from herbs and plants. Standardization of education programs in Herbal medicine and
Aromatic Plants training, Turkey should be done to reach these purposes.

KEYWORDS
Herbal medicine, aromatic plants, education

Corresponding Author: MER ARSLAN KALAFATCILAR, oakalafatcilar@yahoo.com


1
CELAL BAYAR NVERSTES, ALAEHR MESLEK YKSEKOKULU, TIBB VE AROMATK BTKLER
PROGRAMI (EMEKL RETM YES)

1540
Poster Session 12
Submission ID: 1511

EFFECT OF POLYHERBAL FORMULATION (IMMUPLUS AFS) ON


THE HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF ;
FIRST FINDINGS.

1 1
FATIH MEHMET BIRDANE , DURMU FATIH BAER

ABSTRACT
The purpose of this study was to determine the clinical, hematologic and biochemical side
effects of herbal mixture ImmuPlus AFS (Tinospora cordifolia, Withania ashwagandha, Emblica
officinalis, Ocimum sanctum, Andrographis paniculata, Azadirachata indica, and Magnifera india) in
newborn holstein calves (n: 22). Immuplus AFS were given daily at a dose of 0.05 gr / kg for 15 days
starting from the day of birth. Clinical examinations were carried out daily by veterinarians. At the
same time, on days 0, 7, and 20, some biochemical parameters and hematological examinations were
performed on days 0 and 20. Although some differences were found in biochemical parameters, it was
seen that it was within the normal accepted limits (some of them were ALT, AST, ALP, LDH, GGT,
Urea, Total Biluribin, Direct Biluribin, Calcium, Phosphorus, Total Protein, CK, Glucose). In terms of
measured serum and hematological values, Initial findings indicate that ImmuPlus is not an important
side effects in newborn calves.

KEYWORDS
Immune modulator, Tinospora cordifolia, Withania ashwagandha, Emblica officinalis,
Ocimum sanctum, Andrographis paniculata, Azadirachata indica, Magnifera india, Calves.

Corresponding Author: DURMU FATH BAER, dfbaser@aku.edu.tr


1
AFYON KOCATEPE NVERSTES

1541
Poster Session 12
Submission ID: 1512

HERBAL STORAGE MEDIA IN MAINTAINING THE VIABILITY OF


AVULSED TEETH

1 2
BURCU GYETMEZ TOPAL , ZUHAL KIRZIOLU

ABSTRACT
Dental traumas can be seen in various forms, such as crown fracture, root fracture or tooth
avulsion. The avulsed teeth must be stored in a suitable environment in which the viability of the teeth
can be maintained. Many natural and laboratory solutions have been used to maintain the viability of
the avulsed teeth. There have also been studies in which some plants such as teas, medicinal aromatic
plants, fruits, which are frequently used in the medical and pharmacological field, have been evaluated
and found to be effective as a storage medium for the viability of avulsed teeth, as a result of the
popularity of the natural products. In this study, it is aimed to review the easily accessible herbal
storage media such as green tea, wild sage, coconut, red mulberry, rice, propolis, aloe vera in order to
maintain the viability of the avulsed teeth.

KEYWORDS
Medicinal Plants; Dentistry; Trauma

Corresponding Author: BURCU GYETMEZ TOPAL, dt.burcugucyetmez@hotmail.com


1
SERBEST (UZMAN OCUK D HEKM)
2
SLEYMAN DEMREL NVERSTES D HEKML FAKLTES (PROF. DR.)

1542
Poster Session 12
Submission ID: 1513

FATTY ACID COMPOSITION OF SOME HYPERICUM SPECIES BY


USING GC/MS

1 2 3 4 5
MEHMET AKDENIZ , ABDULSELAM ERTA , IIL AYDIN , MUSTAFA ABDULLAH YILMAZ , YETER YEIL , HAMDI
6 1
TEMEL , FIRAT AYDIN

ABSTRACT
Hypericum L. is a relatively large genus of the Hypericaceae family that includes about 484
species of trees, shrubs and herbs. Recently, a growing market niche for Hyperici herba products and
thus an increasing demand for crude material have resulted in considerable research into the
pharmacological activities of the Hypericum genus. Most of this research has focused on Hypericum
perforatum L. (Hypericaceae), which is the most common and best known member of this genus and
has been certified for marketing as a traditional medicine in many European countries. Although
several other Hypericum species are also used as traditional medicinal plants to treat a variety of
ailments, comparatively few studies have been reported for other members of the Hypericum genus.
Hypericum lydium Boiss. (Hypericaceae) is a perennial herb that can reach a height of up to 60 cm
and has yellow flowers and characteristic translucent glandular dots on the sepal margin Known in
Turkish as sanc otu ("cramp herb") and mayasl otu ("hemorrhoid herb"), H. lydium has traditionally
been used in folk medicine to treat menstrual disorders, stomach pains, wounds, hemorrhoids and
indigestion. In this study, the fatty acid composition of aerial parts and root of H. orientale, H.
pruinatum, H. lysimachioides var. spathulatum and H. hyssopifolium var. elongatum were analyzed by
GC-MS/FID. A hundred milligram of the petroleum ether extract was refluxed in 0.1 M KOH solution
in 2 mL of methanol during 1h, the solution was cooled and 5 mL of water was added. The aqueous
mixture was neutralized with 0.5 mL of HCl solution, it was extracted with diethyl ether: hexane (3.5:
1: 1 mL). The separating organic phase was washed with 10 mL water, and dried over anhydrous
Na2SO4. The solvent was evaporated in vacuum and then fatty acid methyl esters were obtained. The
major components of the fatty acid composition were identified as arachidic and cis-13,16-
docosadienoic acids for H. orientale, stearic and cis-13,16-docosadienoic acids for H. pruinatum, H.
lysimachioides var. spathulatum and H. hyssopifolium var. elongatum.

KEYWORDS
Hypericum, Fatty acid, GC-MS, cis-13,16-docosadienoic acid

Corresponding Author: FIRAT AYDIN, faydin@dicle.edu.tr


1
DCLE UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF ANALYTCAL CHEMSTRY, DYARBAKR
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR
3
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY, DYARBAKR
4
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL CHEMSTRY,
DYARBAKIR
5
ISTANBUL UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL BOTANY, 34116,
ISTANBUL
6
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL CHEMSTRY,
DYARBAKIR

1543
Poster Session 12
Submission ID: 1515

EVALUATION OF THE QUALITY PROPERTIES OF MINT SAMPLES


DRIED AT DIFFERENT TEMPERATURES IN CARBON FIBER
ASSISTED CABIN DRYER

1 1 1 2 2
SERPIL PEKDOAN GZTOK , HANDE ZGE GLER , ORHAN KAYA , MUTLU EVIK , SERDAL SABANCI , MER
1 3
FARUK OKGEZME , FILIZ IER

ABSTRACT
Mint (Mentha piperita), a medicinal and aromatic plant, is rich in bioactive compounds. In
addition to wet and dry production of mint, it is widely used as dried due to not be keeping fresh for
long times. Drying temperatures in medical aromatic plants are important to maintain its aroma,
etheric oil, total phenolic content, antioxidant activities and color properties. This study was carried
out to investigate the effect of different drying temperatures on the bioactive (total phenolic content,
antioxidant activity) and color characteristics of the mint samples dried in the carbon fiber assisted
cabinet dryer (CFCD), which has a heat source different from the dryers mentioned in open literature.
For this purpose, mint samples were dried from initial moisture content of 81-83% to 10% in CFCD
system at different drying temperatures (45, 50 and 55 C) with an air velocity of 0.8 m/ s. In order to
determine the antioxidant activity, the EC50 value was taken into consideration which is determined
by the DPPH radical reduction capacity method. L * value indicating the brightness of fresh and dry
mint samples were found between 33.06 and 46.14, and a* values indicating green value were found
between -7.04 and -1.08. It was also observed that as the temperature increased the green color (a*
values) of the samples decreased. The brightness of samples were different than that of raw mint
samples at the temperatures of 45C and 50C, statistically (p <0.05). Besides, it was determined that
the total phenolic content (TPC), which was 103.16 2.66 (mg/g dry matter) in raw material,
decreased to 96.95 2.8, 95.88 1.65, 66.32 3.09 mg / g drymatter for 45, 50, 55C, respectively. It
was found that the drying temperature has an effect on the antioxidant activity (AC) value in the dried
samples; and the highest AC value was observed at 50 C It has been determined that the increase in
temperature for the drying process of mint samples had negative effects on total phenolic, antioxidant
activity and color values. It was recommended that the drying of the mint samples in CFCD should be
conducted below 50C.

KEYWORDS
drying, mint, carbon fiber supported cabinet dryer, quality

Corresponding Author: MUTLU EVK, mutlucevik3538@hotmail.com


1
EGE NVERSTES, FEN BLMLER ENSTTS, GIDA MHENDSL ANABLM DALI
2
MUNZUR NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM
3
EGE NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM

1544
Poster Session 12
Submission ID: 1517

THE EFFECTS OF NITROGEN ON GROWTH AND PHYSIOLOGICAL


FEATURES OF LAVENDER

1 2 2 2
SARA YASEMIN , ASLIHAN OZKAYA , NEZIHE KOKSAL , BEKIR GOK

ABSTRACT
Lavender, in the Lamiaceae family, is an aromatic shrub cultivated worldwide owing to
lavender oil of commerce and is used as an ornamental plant. It is also traditionally believed to be
antibacterial, antifungal, carminative, sedative, treat headaches, antidepressive, diabetes and effective
for burns and insect bites. Plant nutrient requirement is essential for a healthy plant development and,
obtaining high yields. Nitrogen is the most necessary element for the plant growth. In the present study
we focused on determining the effects of different nitrogen treatments on plant growth and
physiological parameters of lavender. For this purpose, 6 different nitrogen concentrations (0, 50, 100,
200, 400 and 800 mg l-1 N; NH4NO3) were treated to lavender plants. Root length, shoot height,
number of branch, stem diameter, root collar diameter and dry and fresh weights of plant biomass
were investigated as plant growth parameters. Leaf chlorophyll concentration (SPAD readings),
relative water content and loss of turgidity were evaluated as physiological parameters. As a result,
root length, thickness of stem and, root collar, fresh weight of shoot and, total plant, leaf chlorophyll
concentration, relative water content and loss of turgidity were significantly affected by nitrogen
treatments, statistically. It was determined that up to 200 mg l-1 N nitrogen levels could apply for
fertilization of lavender plant in pot. Some adverse effects were observed related to growth parameters
in 400 and 800 mg l-1 N treatments while the similar negative effects in terms of leaf chlorophyll
concentration, relative water content and loss of turgidity were detected in 800 mg l-1 N. At the end of
the study, some results were obtained which will form the basis for future studies to determine the
requirement of plant nutrition in lavender plant.

KEYWORDS
Nitrogen, lavender, plant development, plant physiology

Corresponding Author: NEZHE KOKSAL, nkoksal@cu.edu.tr


1
UNVERSTY OF SRT, FACULTY OF AGRCULTURE, DEPARTMENT OF HORTCULTURE, KEZER, SRT,
2
UNVERSTY OF CUKUROVA, FACULTY OF AGRCULTURE, DEPARTMENT OF HORTCULTURE, BALCALI,
ADANA, TURKEY

1545
Poster Session 12
Submission ID: 1518

ESSENTIAL OIL COMPOSITION OF 5 ACHILLEA SPECIES BY GC-


FID-MS

1 1 1 2 1
MUSTAFA ABDULLAH YILMAZ , ABDULSELAM ERTA , OUZ AKIR , ENES ARICA , YILMAZ AYDIN , MEHMET
3 1 1
AKDENZ , SMAIL YENER , HAMDI TEMEL

ABSTRACT
The genus Achillea L. belongs to Asteraceae (Compositae), which is the largest family of
vascular plants and distributed throughout the world. Achillea is represented by more than 140
perennial herbaceous species worldwide and forty-seven species, 24 of which are endemic, grow in
Turkey. Achillea species, commonly known as yarrow, have been used in folk medicine for
thousands of years due to their numerous medicinal properties. Today, several therapeutic
applications, such as anti-inflammatory, wound healing, spasmolytic and choleretic uses, are approved
by scientific experimental results. In Turkey, various species of the genus are used in wound healing;
abdominal pain; stomachache; symptomatic relief of colds, ulcer, and diarrhea; as diuretic;
emmenagog; appetizer; carminative; and insecticidal agent. In this study, essential oil composition of
4 Achillea species (Achillea nobilis L., Achillea goniocephala Boiss. & Balansa, Achillea sintenisii
Hub.-Mor., Achillea coarctata Poir.) growing in Turkey were investigated by GC-FID-MS. As a result
of the GC analysis; eucalyptol, chrysanthenone, 1R-a-pinene, o-cymene, isoborneol and spathulenol
were the main components of A. nobilis L.; eucalyptol, endo-borneol, (-)-camphor, piperitone,
terpineol and 4-terpineol were the main components of A. goniocephala; Beta-eudesmol, piperitone,
caryophyllene oxide and endo-borneol were the main components of A. sintenisii; 2-bornanone,
eucalyptol, endo-borneol and viridiflorol were the main components of A. coarctata.

KEYWORDS
Achillea, essential oil, GC-FID-MS

Corresponding Author: MUSTAFA ABDULLAH YILMAZ, mustafaabdullahyilmaz@gmail.com


1
DCLE UNVERSTY FACULTY OF PHARMACY
2
DCLE UNVERSTY FACULTY OF MEDCNE
3
THE COUNCL OF FORENSC MEDCNE, THE MNSTRY OF JUSTCE

1546
Poster Session 12
Submission ID: 1519

COMPLEMENTARY AND ALTERNATIVE TREATMENT METHODS


IN MALE INFERTILITY

1 2
ENAY TOPUZ , LKNUR MNEVVER GNEN

ABSTRACT
Objective: Increasing infertility cases cause more and more people to try alternative treatment
methods. Aim of In this study determining which alternative ways people tried in case of infertility
due to man. Materials and Methods The data were scanned in the Turkish and English databases
from the Ankara University Library by entering key words such as male infertility, complementary
alternative medicine, infertility treatment. The articles from the last 10 years were evaluated. Results
When studies are examined, it is seen that the use of CAM is increasing day by day in all of the world,
the usage prevalence among women is higher but the prevalence of men infertility is high. When the
studies are examined, it is seen that men try to lose weight with diet and exercise, Alcohol and
smoking stopped in order to increase the number and quality of sperm.In randomized controlled trials
with small groups, some studies of acupuncture, vitamin C, vitamin E, saffron and scrotal cooling
suggested that a combination of coenzyme Q10, glutathione, Korean red ginseng, omega-3, selenium,
zinc and folate and Menevit antioxidant supplementation had a positive effect But it is emphasized
that there is no work at the level of evidence. In a very large rewiev study (scanning of textbooks
between 815 and 1901) it was stated that 180 plant species were effective on the male reproductive
system, but when it was shown that toxic effects were reached, 36 plants were reached and the
common feature of these 36 plants was to increase libido . Some of these plants (Boswellia carterii,
Chlorophytum borivanianum, Alternative viagra, Phyllanthus emblica, Withania somnifera, Black
cumin, Ferula Assa-foetida, Crocus sativus (saffron), Commiphora mukul (guggul)) are recommended
in the treatment of varicocele induced conditions. Studies done with selenium especially in
infertility treatment are excessive. After selenium-rich diet, there was a decrease in sperm motility and
thyroid hormone levels. However, there are studies that indicate that they have increased. While there
are studies showing that acupuncture is effective, it is very limited for yoga. Conclusion: It is a fact
that complementary and alternative medical practices have been used for men's sexuality and
infertility treatment since the past, including many agents and pathways. However, evidence-based
work is limited. For this reason, there is a need for evidence-based studies on large groups.

KEYWORDS
infertility, male infertility, complemantary and alternative treatment

Corresponding Author: ENAY TOPUZ, senaytopuz@gmail.com


1
ANKARA NVERSTES SALIK BLMLER FAKLTES

1547
Poster Session 12
Submission ID: 1520

PHYTOCHEMICAL PROPERTIES OF GRAPE SEED OIL AND THE


EFFECTS ON HEALTHY LIFE

1
NAZAN BALBABA

ABSTRACT
Our country has an important share among other countries with regard to grape growing.
Grape seeds are by-products of wine industry with fruit juice and molasses industry. Grape seeds
contain fiber, oil, protein, carbohydrate, phenolic compounds and mineral substances. Grape seeds are
quite rich in terms of the phytochemicals which contains. The phytochemicals provide protection from
many diseases by strengthening the immune system. The amount of oil in grape seeds is between 11-
22% to varies with the cultivars. 90% of grape seed oil is composed of mono- and polyunsaturated
fatty acids. The highest level of fatty acid is linoleic acid. This is followed by oleic acid, palmitic acid,
stearic acid and linolenic acid, respectively. Which are rich in linoleic acid, grape seed oil, LDL (low
density lipoprotein) as the cause of the fall is help protect cardiovascular health. Obtained by different
methods and very useful for health of grape seed oil is also used in the cosmetic industry and
aromatherapy. The amount of vitamin E found in grape seed oil is dependent on variety and
environmental factors. Due to the high antioxidant effect of vitamin E has preservation of vascular
health, Alzheimer's disease and cancer risk and therapeutical effects of tumors. Therefore, the use of
grape seed oil has been suggested as anti-aging and for the prevention of chronic diseases.

KEYWORDS
Grape seed oil, fatty acids, antioxidant, vitamin E, phytochemicals

Corresponding Author: NAZAN BALBABA, dogar@ksu.edu.tr


1
KAHRAMANMARA ST MAM NVERSTES TRKOLU MYO, TIBB VE AROMATK BTKLER
PROGRAMI

1548
Poster Session 12
Submission ID: 1521

DEVELOPMENT OF A NEW PERFORMANCE TEST METHOD TO


EVALUATE THE EFFICACY OF HERBAL SHAMPOO FOR HAIR
GRAYING

1 2 1 1
EBRU ALTUNTA , BURCU ZKAN , FEDA DALO , GLGN YENER

ABSTRACT
Hair is one of the most important components for the general appearance of each of us. Hair
has been regarded as an expression of the personality and the social role for centuries. In many
civilizations throughout history, hair has been mentioned positive attributes such as beauty and power.
Today, everyone wants to get well-groomed hair without the gender difference. Hair graying, one of
the leading hair problems, is associated with ageing and assessed negatively. The psychology of
persons experiencing this problem is affected negatively and results in the decrease of confidence. The
hair graying may occur due to factors such as heredity, thyroid problems and malnutrition. Hair
graying is the whitening of hair as a result of the decrease of the colouring agent in the hair roots
called melanin, which provides the hair its colour, the deceleration and even stagnation of its synthesis
mechanism (1). When the melanin in the hair structure decreases, air sacs are formed instead of
pigment that gives color to the hair and this condition leads to graying of the hair. One of the reasons
that reduces melanin is the deficiency or lack of vitamin B. Disorders such as stress, depression,
irritability, too much consumption of excessive sugary products, white flour products and animal
nutritions are known to increase vitamin B deficiency. Animal nutritions make difficult of the
absorption of vitamins due to causing excessive lipoidosis. All these factors lead to an accumulation of
hydrogen peroxide that is known to be a major cause of hair graying in hair follicles. Air sacs
accumulate from the roots towards the ends of the hair and the hair gets gray since the accumulated
hydrogen peroxide at the hair roots prevent the melanogenesis mechanism (2). The hair cosmetic
industry has undergone a revolutionary change over the last two decades. One of the new type of hair
cosmetics is gradual hair dye. This is home-use product which give the desired color after several days
of use. The product has to be applied daily on the hair strand till the desired shade is reached (3). The
objective of this study was to develop a new method in order to measure coloring efficacy of an anti-
gray shampoo containing natural ingredients. For this purpose, we developed an in-vitro performance
test method to compare this product against a commercially available anti-gray shampoo (Alpecin
Tuning shampoo). White Caucasian hair swatches were used for the test. To determine melanin
content, color measurements were taken with Mexameter MX16 before washing process as a reference
and after five washes. After color measurements with Mexameter MX16, photos were taken from the
hair tresses for comparison.

KEYWORDS

Corresponding Author: BURCU ZKAN, burcu_ozkan93@hotmail.com


1
STANBUL NVER STES, ECZACILIK FAKLTES
2
YILDIZ TEKNK NVERSTES, BYOMHENDSLK BLM

1549
hair graying, cosmetic, anti-gray shampoo, topical

1550
Poster Session 12
Submission ID: 1523

UTILIZATION OF TARHANA HERB (ECHINOPHORA


SIBTHORPIUNA) IN TARHANA PRODUCTION

1 1 1
AYLA SARI , ALI GNC , ALI KEMALI ZUUR

ABSTRACT
Tarhana is a nutritious food substance that is produced by mixing wheat products with
yoghurt, some vegetables and spices, fermentation of both lactic acid and ethyl alcohol, and drying
after fermentation to increase the storage time. Tarhanan's production pattern is similar to most
countries and regions, but may vary in composition depending on tradition, custom and nutrition
habits, as well as grain, legumes and vegetable varieties that vary from region to region. Tarhana herb
is also used in some regions to provide flavor and aroma to tarhana. Tarhana otu (Echinophora
sibthorpiuna) is a plant with yellow flowers, 20 to 45 cm in length and a perennial plant. A total of six
species, three of which are endemic, are also known as tarhana otu, which is grown in Turkey, with the
names "rtk", "rdk" and "Turu Otu". They are fresh or dried in the production of meat, pickles,
helvardas, soups and tarhana. It is useful in the digestive system. Stomach is good for ulcers. It treats
mental and psychological diseases. It gives positive results in asthma, bronchitis, hay fever, kidney
stones, rheumatism and eczema treatments. In addition, they are also considered as drugs in the
treatment of fungi. Due to the essential oils it contains, it has its own aromatic taste and is used as a
flavor for this purpose in tarhans. They increase the amount of ash in the food that they are
contributing to the nutritional value. In Tarhana, they are used between 0,5% and 1% of total tarhana
dough mass.

KEYWORDS
Tarhana, herb, rtk.

Corresponding Author: AL GNC, ali.goncu@adu.edu.tr


1
ADNAN MENDERES NVERSTES, NE MESLEK YKSEKOKULU, GIDA LEME BLM, AYDIN.

1551
Poster Session 12
Submission ID: 1526

THE ESSENTIAL OIL COMPOSITION OF HYPERICUM SCABRUM


COLLECTED FROM DIFFERENT LOCALITIES

1 1 2 3 4
MEHMET AKDENZ , FIRAT AYDIN , IIL AYDIN , MEHMET FIRAT , SMAIL YENER , MUSTAFA ABDULLAH
5 6 6 7
YILMAZ , LEYLA BALUR , MEHMET VEYSI ALAYAN , ABDULSELAM ERTA

ABSTRACT
Hypericum (Hypericaceae) is one of the 100 largest genera that comprise an estimated 22% of
angiosperm diversity) with the presence of 484 species from 36 taxonomic sections. Hypericum
species are wellknown healing agents in traditional medicine due to various medicinal properties.
Despite the large number of Hypericum species, only Hypericum perforatum L. has been studied in
depth as pharmaceutically important medicinal crop plant which extracts widely used in Europe as a
drug for the treatment of mild and moderate depression. Turkey is an important prevalence centre of
Hypericum species. According to the most recent count by Gner et al. (2012), there are a total 96
Hypericum species in the flora of Turkey, 46 of which are endemic. All Hypericum species have been
used in Turkish folk medicine under the names kantaron, peygamber iei, klotu, kanotu,
kuzukran and binbirdelik otu as sedatives, antiseptics and antispasmodics. Curative value of
Hypericum plants have been mainly attributed to the phytochemicals, namely phloroglucinol
derivatives, naphthodianthrones, different phenolic compounds and essential oils which possess a
great variety of bioactivities. In this study, the essential oil contents of H. scabrum collected from
different was analyzed by GC-MS/FID. The dried aerial parts of H. scabrum species were cut into
small pieces and subjected to hydro- distillation with water for 4 h, using a Clevenger-type apparatus
to produce essential oils which were dried over anhydrous sodium sulphate and sto
required. The essential oils were diluted by dichloromethane (1:3, v/v) before the GC run.
Identification of the compounds was based on the comparison of their retention times and mass spectra
with those obtained from authentic samples and/or the NIST and Wiley spectra as well as the literature
data. The samples collected from the four different localities these all shows similar varieties as
essential oil content. But when we looked at -pinen contents, the samples specially collected from
Kahramanmara have quite higher -pinen contents than the samples collected from Nevehir, Van
and Elaz.

Corresponding Author: MEHMET AKDENZ, mehmetakdeniz21@hotmail.com


1
DCLE UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF ANALYTCAL CHEMSTRY,
DYARBAKR,TURKEY
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY,
DYARBAKR,TURKEY
3
YZNC YIL UNVERSTY, FACULTY OF EDUCATON, DEPARTMENT OF BOLOGY, VAN, TURKEY
4
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY,
DYARBAKR,TURKEY
5
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL CHEMSTRY,
DYARBAKIR,
6
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY,
DYARBAKR,TURKEY
7
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR,
TURKEY

1552
KEYWORDS
Hypericum scabrum, Essential Oil, GC-MS/FID

1553
Poster Session 12
Submission ID: 1527

NURSING STUDENTS USAGE STATUS AND OPINIONS ABOUT


MEDICINAL AND AROMATIC PLANTS

1 1
ALIYE AYIR , HAMIDE AYGR

ABSTRACT
OBJECTIVE: Increase in diseases that are difficult to maintain and treat has been observed in
parallel with the increase in human life span. The high cost of new technologies used in the treatment
of these diseases, obscurity about current care and treatment methods and fears about possible side
effects have significantly increased the tendency to use medical and aromatic plants. Turkey has a
wide variety of medical aromatic plants and many medical and aromatic plants which have been used
for the protection, maintenance of health and therapeutic purposes. Therefore this study was planned
to determine the use of medicinal and aromatic plants by nursing students and their opinions on these
plants. METHOD: This descriptive research was conducted at Necmettin Erbakan University in the
spring semester of 2016-2017 academic year. The universe of the research consisted of 370 students
who are in 1st, 2nd, 3rd and 4th classes of Department of Nursing in Faculty of Health Sciences of
Necmettin Erbakan University in 2016-2017 academic year. Sample selection was not made in the
study and it was completed with 257 students who volunteered to participate in the survey. The
research data were collected by a questionnaire consisting of 21 questions prepared by the researchers
as a result of literature review. The data was analyzed with the use of percentage and mean.
RESULTS: The average age of the students was 201.82, 80.2% of them were female, 61.5% of them
resided in student residence and 90.7% of them did not have any chronic disease. 93% of the students
stated that medicinal and aromatic plants are beneficial and 72.8% of them stated that they prefer the
use of medicinal and aromatic plants when they are sick, and 84% of them stated that they use
medicinal and aromatic plants with the advice of their mother, father or other elders in their families.
The most common medicinal and aromatic plants used by the students were determined as linden
(61.1%), mint (43.6) and sage (42.8%). It was found that 78.2% of the students stated that medical and
aromatic plants strengthen the immune system, 86.4% of the students stated that sufficient research
was needed to use these plants, 66.9% of the students stated that these plants increase motivation while
studying, 73.5% of the students stated that these plants decrease the physicological stress in the classes
and exams and 81.3% of the students stated that these plants can be used as sedative. CONCLUSION:
It has been determined that students often use medicinal and aromatic plants with the advice of their
elders. It is recommended to raise awareness of the community on such issues as getting
recommendations from reliable sources for access to these plants and using them on appropriate
criteria.

KEYWORDS
Medical, aromatic, plants, nursing students

Corresponding Author: ALYE AYIR, aliye.cyr@gmail.com


1
NECMETTN ERBAKAN NVERSTES SALIK BLMLER FAKLTES

1554
Poster Session 12
Submission ID: 1528

USE OF SOLVENT-FREE MICROWAVE METHOD FOR


EXTRACTION OF ESSENTIAL OILS

1 1
AYSUN YURDUNUSEVEN YILDIZ , HAKAN KARACA

ABSTRACT
Essential oils are complex mixtures of volatile compounds with strong odor that are extracted
from different parts of plants, for example, leaves, peels, barks, flowers, buds, seeds, roots etc. So far,
approximately 3000 essential oils have been discovered, about 300 of which are commercially
important. Essential oil components are synthesized in plant by the secondary metabolisms and stored
in glandular trichomes, oil cells or ducts in plant tissue. In general, essential oils are mixture of
terpenes, aromatic compounds, and terpenoids. Essential oils have been widely used for bactericidal,
virucidal, fungicidal, antiparasitical, insecticidal, medicinal, pharmaceutical, agricultural and food
fields. The conventional methods for extraction of essential oil widely used by the industry are steam
distillation, hydrodistillation and solvent extraction. These methods have various disadvantages which
greatly effects the yield and quality of the essential oil. Although these methods have been used for
many years for essential oils extraction, these applications have some disadvantages in the product
such as losses of some volatile compounds, low extraction efficiency, possible toxic solvent residues
and damage or alter the action of chemical structure of the bioactive compounds. Therefore,
researchers are in search of new technologies, which use less solvent, time and energy. Recently
several new techniques like supercritical fluid extraction, pressurized liquid extraction, pressurized hot
water extraction, membrane-assisted solvent extraction, solid-phase microextraction, solvent-free
microwave extraction, microwave-assisted, and ultrasound-assisted extraction have been developed.
Green extraction methods suggest the use of non-toxic solvents for more natural products. Among
them, solvent-free microwave extraction (SFME), have become a good option in research on essential
oil extractions from plants. The advantages of using SFME are faster energy transfer, shorter time and
more environmentally friendly compared to conventional extraction methods. SFME is a combination
of microwave heating and dry distillation, performed at atmospheric pressure. In this method, plant
material is placed into a microwave reactor, without addition of any solvents including water.
Essential oil is evaporated by the in situ water of the plant material. A cooling system outside of the
microwave oven condenses the distillate. In this review, a short theoretical background of SFME and
the basic principles of the method have been presented. Finally, the potential of the SFME technique
has been compared with conventional methods in terms of the extraction of essential oils from
aromatic plants.

KEYWORDS
Solvent-free microwave extraction (SFME), Essential oils, Green extraction

Corresponding Author: AYSUN YURDUNUSEVEN YILDIZ, ayurdunuseven@pau.edu.tr


1
PAMUKKALE UNVERSTY, FACULTY OF ENGNEERNG, DEPARTMENT OF FOOD ENGNEERNG

1555
Poster Session 12
Submission ID: 1532

THE DPPH FREE RADICAL SCAVENGING ACTIVITY AND ABTS


CATION RADICAL DECOLORISATION ACTIVITIES OF ETHANOL
EXTRACTS OF AERIAL PARTS AND ROOT OF HYPERICUM
PERFORATUM COLLECTED FROM DIFFERENT LOCALITIES

1 1 2 3 4 5
MEHMET AKDENZ , FIRAT AYDIN , IIL AYDIN , YETER YEL , KEREM ENTRK , SERKAN YTKAN ,
5
ABDULSELAM ERTA

ABSTRACT
Synthetic and natural antioxidants are of particularly importance in maintaining the oxidative
stress level under the critical point in human organism. Previous in vitro and in vivo studies reported
the antioxidant capacity of several species of medicinal plants, acts at cellular level, through cell
growth stimulation, membrane potential stabilizing or at molecular level, through ROS scavenging,
lipid peroxidation, etc. These roles have been attributed, in part, to their biological active constituents,
such as liposoluble and watersoluble vitamins (E and C, respectively) and polyphenolic substances. As
plants produce a significant amount of antioxidants to prevent oxidative stress, they represent a
potential source of new compounds with antioxidant activity. Some of these plants are those of the
Hypericum genus, (Clusiaceae Lindley, syn. Guttiferae A.L. de Jussieu). They include a large number
of species distributed worldwide. Several of the botanical species belonging to the genus are used in
folk medicine and among them Hypericum perforatum L., also named St. Johns Wort, is especially
known as a traditional remedy for the treatment of melancholia, abdominal and urogenital pain and
ulcerated bums. Over the last two decades, it has been demonstrated that H. perforatum is effective as
an antidepressant, an antiviral, and an antimicrobial. The drug contains peculiar chemical constituents
such as naphthodianthrones (hypericins), acylphloroglucinols (hyperforin), flavonol glycosides
(quercetin derivatives) and biflavones and all the metabolites seem to contribute to its pharmacological
activity. Current use of H. perforatum is mainly for the treatment of mild to moderate depression, and
drug extracts for antidepressant applications have become increasingly popular. During the recent
years, several phytochemical studies on other species of Hypericum have also been performed, leading
to the isolation of new molecules, some having biological activity. The ethanol extracts of aerial parts
and root of H. perferatum species collected from different localities (Nevehir, Diyarbakr, Van,
Kahramanmara and Siirt) were tested for antioxidant (DPPH free radical scavenging activity and
ABTS cation radical decolorisation) activities in this study. Studied ten extracts were found quite on

Corresponding Author: MEHMET AKDENZ, mehmetakdeniz21@hotmail.com


1
DCLE UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF ANALYTCAL CHEMSTRY,
DYARBAKR,TURKEY
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY,
DYARBAKR,TURKEY
3
ISTANBUL UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL BOTANY, 34116,
ISTANBUL,TURKEY
4
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL TOXCOLOGY, 21280
DYARBAKIR,TURKEY
5
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR,
TURKEY

1556
active on used both two antioxidant activity methods. Also, it was determinated that collections from
different localities of studied samples are very important for their antioxidant activity.

KEYWORDS
Hypericum perferatum, DPPH, ABTS

1557
Poster Session 12
Submission ID: 1534

GUNDELIA (KENGER) SPECIES NATURALLY GROWN IN TURKEY


AND USED FOR MEDICAL PURPOSES

1 2 1
EBRU YCE-BABACAN , RIDVAN POLAT , UUR AKILCIOLU

ABSTRACT
In Turkey, the naturally spreading Gundelia genus is represented by 3 varieties (Gundelia
tournefortii L. var. tournefortii L. var. Freyn et Sint, var. tenuisecta Boiss.). With the studies made in
recent years, 9 species belonging to this genus have been identified in Trkiye. Of these species, 7 are
endemic (G. munzuriensis Vitek, Yce & Ergin, G. dersim Vitek, Yce & Ergin, G. vitekii Armagan,
G. komagenensis Frat, colemerikensis Frat, G. cilicica Frat ve G. anatolica Frat) and 2 are new
record (G. glabra Mill. ve G. rosea M.Hossain & Al-Taey) for Turkey. In this review, information is
given about 9 Gundelia species which are naturally distributed in Turkey.

KEYWORDS
Gundelia, kenger, endemic, Turkey, flora.

Corresponding Author: EBRU YCE-BABACAN, ebruyucee@yahoo.com


1
MUNZUR NVERSTES
2
BNGL NVERSTES

1558
Poster Session 12
Submission ID: 1535

USE OF THYME, BAY, PEPPERMINT AND ROSEMARY ESSENTIAL


OILS AS NATURAL ANTIMICROBIAL AGENTS FOR FOOD
PACKAGING

1 1
AYSUN YURDUNUSEVEN YILDIZ , HAKAN KARACA

ABSTRACT
Aromatic plants are used since ancient times due to their flavor, preservative, and medicinal
properties. Aromatic plants synthesize many compounds with complex molecular structures by their
secondary metabolisms. These secondary metabolites are alkaloids, flavonoids, isoflavonoids, tanins,
cumarins, glycosides, terpens and phenolic compounds. Essential oils are obtained from aromatic
plants such as oregano, rosemary, thyme, lavender, or peppermint. Essential oils are natural, volatile
and complex compounds characterized by a strong odor soluble in lipid and organic solvents. Essential
oils have various bioactivities including antibacterial, antiviral, antiinflammatory, antifungal,
antimutagenic, anticarcinogenic, and antioxidant activities depending on the quality and quantity of
their components. In general, essential oils have two or three major components in high concentrations
and other components present in trace amounts. These major components of essential oils provide
their biological activities. For instance, usually the main components in essential oils are carvacrol and
thymol in thyme oil, 1,8-cineole in bay oil, menthol, menthone and menthofuran in peppermint oil and
1,8-cineole and -pinene in rosemary oil. Thyme (Thymus vulgaris L.), bay (Laurus nobilis L.),
peppermint, (Mentha piperita L.) and rosemary (Rosmarinus officinalis L.) are generally used in
dishes for their flavors. They are also among the most commonly investigated plants for their
preventive properties against microbial and chemical deterioration. The number of studies showing the
possibility of using essential oils in food systems to prevent the growth of foodborne bacteria and to
extend the shelf life of the food. The antimicrobial activity of essential oils is mostly due to the
presence of phenols, aldehydes, and alcohols. Essential oils affect the cell membrane of the pathogenic
microorganism. The plants themselves or their extracts can be included into the formulation of food
products. One of the main problems about using essential oils of the plants are their negative effects on
the sensory properties of the product. In many cases, they make the food undesirable due to their
dominant aroma. This problem can be overcome by using the essential oils in the food packaging
material rather than including them directly to the formulation of the food. By this way, the release of
the bioactive compounds in the essential oils can be controlled and products with acceptable sensory
properties and extended shelf life can be obtained. The aim of this study is to provide an overview of
the studies concerning the food packaging applications including essential oils of aromatic plants.

KEYWORDS
Food packaging, Essential oils, Aromatic plants

Corresponding Author: AYSUN YURDUNUSEVEN YILDIZ, ayurdunuseven@pau.edu.tr


1
PAMUKKALE UNVERSTY, FACULTY OF ENGNEERNG, DEPARTMENT OF FOOD ENGNEERNG

1559
Poster Session 12
Submission ID: 1536

ANTIMICROBIAL ACTIVITY AND BIOCHEMICAL ANALYSIS OF


IBERIS HALOPHILA

1 2 3 4
ZCAN MEK , ALI YETGN , KEREM CANLI , ERGIN MURAT ALTUNER

ABSTRACT
Iberis halophila is rare endemic species in Central Turkey, so related research doesnt exist.
Antimicrobial activity was investigated against 17 bacteria and 1 fungi by using disk diffusion
method. These microbial strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella,
Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida geniuses. Twelve of them are
standard species and they are important for exact determination of antimicrobial activity. 4.94, 9.87
and 19.74 mg samples were prepared by using ethanol extraction method. Also, bioactive composition
of this sample was determined by Gas Chromatography-Mass Spectroscopy and National Institute of
Standards and Technology (NIST) library was used for mass spectra analysis. The results were
presented that I. halophila has antibacterial activity against eight of the tested strain. Several active
metabolites were identified, but some composition of this sample is not match with library. Unknown
molecule should be analyzed by NMR spectra for 3d structure determination and identification. These
results are the first report for the antimicrobial potential and chemical composition of I. halophila.

KEYWORDS
Iberis halophila, endemic plant, antimicrobial activity, bioactive composition, ethanol extract

Corresponding Author: ZCAN MEK, ozcan.simsek@gmail.com


1
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, ANKARA UNVERSTY, TR 06100, ANKARA, TURKEY
2
DEPARTMENT OF BOTECHNOLOGY, INSTTUTE OF ENGNEERNG AND SCENCE, IZMR INSTTUTE OF
TECHNOLOGY, IZMR, TURKEY
3
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, DOKUZ EYLL UNVERSTY, IZMR, TURKEY
4
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE AND ARTS, KASTAMONU UNVERSTY, TR 37150,
KASTAMONU, TURKEY

1560
Poster Session 12
Submission ID: 1537

MUNZUR GARLIC (ALLIUM TUNCELIANUM (KOLLMANN)


ZHATAY, B.MATHEW & SIRANECI) AND TRADITIONAL USE

1 2 1
EBRU YCE-BABACAN , RIDVAN POLAT , UUR AKILCIOLU

ABSTRACT
The most important feature of Munzur Garlic is that it is single-threaded or rarely two-
threaded. It blooms in June and July. The garlic is collected by local people for use in their homes and
marketting in August and September. It is traditionally used for food, medical and insect repellent.
Munzur Garlic grows naturally on the slopes of Munzur Mountain, Tunceli /Plmr, Erzincan and
Sivas. The plant is under protection because of unconscious and excessive use. It is a kind of
economic support to the people of the region when cultural studies are increased. In this review, we
will be given information about the medical and economic value of Munzur garlic.

KEYWORDS
Munzur, garlic, Allium tuncelianum, traditional use.

Corresponding Author: EBRU YCE-BABACAN, ebruyucee@yahoo.com


1
MUNZUR NVERSTES
2
BNGL NVERSTES

1561
Poster Session 12
Submission ID: 1538

ANTIMICROBIAL ACTIVITY AND BIOCHEMICAL ANALYSIS OF


FRANKENIA HIRSUTA

1 2 3 4
ZCAN MEK , ALI YETGN , KEREM CANLI , ERGIN MURAT ALTUNER

ABSTRACT
Frankenia hirsuta is widely located in Turkey, but antimicrobial potential of it wasnt
determined. Broad range of microorganisms was used for this determination. By using disk diffusion
method, 17 bacteria and 1 fungi susceptibility was analyzed and these microbial species are Bacillus
subtilis ATCC 6633, Candida albicans ATCC 10231, Enterobacter aerogenes ATCC13048,
Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli
ATCC 25922, Escherichia coli CFAI, Klebsiella pneumoniae, Listeria monocytogenes ATCC 7644,
Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella
typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B,
Staphylococcus epidermidis DSMZ 20044 and Streptococcus agalactiae DSMZ 6784. 0.77, 1.54 and
3.08 mg samples were prepared by using ethanol extraction method. Also, bioactive composition of
this sample was determined by Gas Chromatography-Mass Spectroscopy and National Institute of
Standards and Technology (NIST) library was used for mass spectra analysis. The results were
presented that F. hirsuta has antimicrobial activity against all of the studied study except E. aerogenes,
E. coli. Two of them have high susceptible (higher than 15 mm); eleven of them have moderate
susceptible (14-10 mm) and only three of them have low susceptible (9-7 mm). Several active
metabolites were identified, but some composition of this sample is not match with library. Unknown
molecule should be analysed by NMR spectra for 3d structure determination and identification. These
results are the first report for the antimicrobial potential and chemical composition of F. hirsuta.

KEYWORDS
Frankenia hirsuta, antimicrobial activity, bioactive composition, ethanol extract.

Corresponding Author: ZCAN MEK, ozcan.simsek@gmail.com


1
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, ANKARA UNVERSTY, TR 06100, ANKARA, TURKEY
2
DEPARTMENT OF BOTECHNOLOGY, INSTTUTE OF ENGNEERNG AND SCENCE, IZMR INSTTUTE OF
TECHNOLOGY, IZMR, TURKEY
3
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, DOKUZ EYLL UNVERSTY, IZMR, TURKEY
4
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE AND ARTS, KASTAMONU UNVERSTY, TR 37150,
KASTAMONU, TURKEY

1562
Poster Session 12
Submission ID: 1540

QUINOA (CHENOPODIUM QUINOA WILLD.) AS FUNCTIONAL


FOODS

1 1 1
BELKIS TEKGLER , LKAY KOCA , SEBIHA KITAN

ABSTRACT
Quinoa is a plant crop in the Andean mountains in South America. Recently, there has been
increased interest for the product in the United States, Europe, and Asia. Quinoa, which is called as
Chenopodium quinoa Willd. in Amaranthaceae, is commonly mentioned as a pseudo-cereal. Both
leaves and seeds of quinoa are used in human nutrition. Its leaves are consumed as a vegetable. Its
seed is used like cereal crops, and can be milled into flour. Quinoa is an excellent example for
functional food. The functional properties are originated from its proteins, antioxidants, fatty acids,
minerals and vitamins. Quinoa has a high quality protein content and well-balanced amino acid
composition. It is rich in lysine and methionine. Quinoa has not gluten. It is recommended for celiac
patients because of its gluten-free nature. It is rich in saponins and antioxidants such as phenolics. Its
composition particularly gives a strong contribution to human nutrition. It reduces the risk of various
diseases. In this review, the functional potential and nutrient components of quinoa are discussed.

KEYWORDS
Quinoa, functional food, celiac

Corresponding Author: LKAY KOCA, itosun@omu.edu.tr


1
ONDOKUZ MAYIS NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM, 55139,
SAMSUN

1563
Poster Session 12
Submission ID: 1542

DETERMINATION OF BIOACTIVE COMPOSITION AND


ANTIMICROBIAL ACTIVITY OF RANUNCULUS FICARIA

1 2 3 3 4
KEREM CANLI , ALI YETGN , ILGAZ AKATA , ZCAN MEK , ERGIN MURAT ALTUNER

ABSTRACT
Medical herbs have many bioactive component and they are used in microbial treatment since
ancient time. Ranunculus ficaria one of them and it is important for folkloric medicine in Turkey. It is
also called lesser celandine and it is native species of west Asia and Europe. Ranunculus ficaria
investigation were applied against 17 bacteria and 1 fungi by using disk diffusion method. These
microbial strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria,
Pseudomonas, Salmonella, Staphylococcus and Candida geniuses. Twelve of them are standard
species and they are important for exact determination of antimicrobial potential. Bioactive
composition of this sample was also determined by Gas Chromatography-Mass Spectroscopy and
National Institute of Standards and Technology (NIST) library was used for mass spectra analysis. 5.2,
10.4 and 13 mg samples were prepared by using ethanol extraction method. The results were presented
R. ficaria has antimicrobial activity against nine tested species. Three of them have high susceptible
(more than 15); three of them have moderate susceptible (14-10 mm) and three of them have low
susceptible (9-7 mm). Several active metabolites were identified, but some composition of this sample
is not match with library. Unknown molecule should be analyzed by NMR spectra for 3d structure
determination and identification.

KEYWORDS
Ranunculus ficaria, medicinal plant, antimicrobial activity, bioactive composition, ethanol
extract

Corresponding Author: KEREM CANLI, biyoloji@gmail.com


1
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, DOKUZ EYLL UNVERSTY, IZMR, TURKEY
2
DEPARTMENT OF BOTECHNOLOGY, INSTTUTE OF ENGNEERNG AND SCENCE, IZMR INSTTUTE OF
TECHNOLOGY, IZMR, TURKEY
3
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, ANKARA UNVERSTY, TR 06100, ANKARA, TURKEY
4
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE AND ARTS, KASTAMONU UNVERSTY, TR 37150,
KASTAMONU, TURKEY

1564
Poster Session 12
Submission ID: 1543

ANTIMICROBIAL ACTIVITY SCREENING OF PLAGIOMNIUM


ELATUM

1 2 3 4
KEREM CANLI , ALI YETGN , ILGAZ AKATA , ERGIN MURAT ALTUNER

ABSTRACT
In the East Asia, bryophytes are mostly used as economical purpose and their cultivation is
applied in moss gardening. Although bryophytes antimicrobial potential is known, most of them are
not determined. Bryophyte contain active substances which are significant for healing of pathogen
infection. The antimicrobial activity of Plagiomnium elatum was investigated against 17 bacterial and
1 fungal species with disk diffusion method. These microbial strains include Bacillus, Enterobacter,
Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and
Candida geniuses. Standard species is crucial for antimicrobial screening, therefore twelve of the
tested microorganisms are selected form standard species. 0.34, 0.68 and 1.13 mg samples were
acquired with ethanol extraction method. Our present study has shown that the ethanol extract of P.
elatum has antimicrobial activity against nine of the studied strains. This analysis is the first report for
the antimicrobial potential of P. elatum.

KEYWORDS
Plagiomnium elatum, bryophyte, antimicrobial activity, disk diffusion method, ethanol extract

Corresponding Author: KEREM CANLI, biyoloji@gmail.com


1
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, DOKUZ EYLL UNVERSTY, IZMR, TURKEY
2
DEPARTMENT OF BOTECHNOLOGY, INSTTUTE OF ENGNEERNG AND SCENCE, IZMR INSTTUTE OF
TECHNOLOGY, IZMR, TURKEY
3
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, ANKARA UNVERSTY, TR 06100, ANKARA, TURKEY
4
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE AND ARTS, KASTAMONU UNVERSTY, TR 37150,
KASTAMONU, TURKEY

1565
Poster Session 12
Submission ID: 1544

SOME NATURAL PLANTS USED MEDICINALLY BY THE PUBLIC IN


BOLVADIN, AY AND SULTANDAI (AFYONKARAHISAR)

1 1
MUSTAFA KARGIOLU , NURGL GRBZ

ABSTRACT
This oral presentation is on medicinal uses of natural plants spreading on districts of
Afyonkarahisar: Bolvadin, ay and Sultanda, which are located in Inner-Western Aegean region of
Turkey. The subject of this presentation is a part of our ongoing master's thesis in which we
investigate traditional uses of natural plants in Afyonkarahisar. In our study we identified 50 plant
species belonging to 20 plant families as medicinal and here we included some of them. For the study
we interviewed with 57 people from 10 villages, 8 towns and 3 district centers. From these people,
information on the uses of plants was obtained.

KEYWORDS
Medicinal plants, Etnobotany, Bolvadin, ay, Sultanda

Corresponding Author: MUSTAFA KARGIOLU, kargi@aku.edu.tr


1
AFYON KOCATEPE UNVERSTY

1566
Poster Session 12
Submission ID: 1545

ANTIOXIDANT PROPERTIES OF FLAVOURED BAGGED TEA AND


FLAVOURED ICE TEA

1 1
BELKIS TEKGLER , LKAY KOCA

ABSTRACT
Black and green tea are produced with aromatized or without aromatized. In our country, for
flavoured tea production process, some aroma compounds like mint, bergamot, jasmine and lemon are
used. In this study, the total phenolic contents and antioxidant activities of flavoured bagged tea and
flavoured ice tea samples were analyzed. For this purpose, bagged tea samples, including 9 flavoured
green and 5 flavoured black tea samples, and 15 flavoured ice tea which are ready to drink were
evaluated. Total phenolics were determined by Folin-Ciocalteu method. Ferric reducing/antioxidant
power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity (as EC50) methods
were used to analyze the antioxidant activity. In the bagged tea samples, the total phenolic contents
were between 36.89 mg GAE/g (green tea with jasmine) and 88.29 mg GAE/g (green tea with
bergamot), FRAP values were between 60.44 mol Fe2+/g (black tea with bergamot) and 557.18
mol Fe2+/g (green tea with jasmine). The lowest EC50 values were determined as 1.69 g/L for
green tea with lemon, while the highest EC50 values were 104.25 g/L in black tea with bergamot.
In the flavoured ice tea samples, the lowest total phenolic, FRAP and EC50 values were 170.81 mg
GAE/L (ice tea with lemon-ginger), 279.43 mol Fe2+/L (ice tea with lemon-ginger) and 5.45 g/mL
(ice tea with lemon), respectively. The highest total phenolic, FRAP and EC50 values were 872.06 mg
GAE/L (lemon ice tea), 8445.08 mol Fe2+/L (ice tea with lemon) and 86.85 L/mL (ice tea with
lemon-ginger), respectively. According to the results of the analysis, the consumption of flavoured
bagged tea and flavoured ice tea contribute to the daily intake of antioxidants.

KEYWORDS
Flavoured tea, ice tea, antioxidant, total phenolic

Corresponding Author: BELKIS TEKGLER, belkisg@omu.edu.tr


1
ONDOKUZ MAYIS NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM, 55139,
SAMSUN

1567
Poster Session 12
Submission ID: 1546

DETERMINATION SOME ORGANOLEPTIC PROPERTIES OF


BISCUITS SUPPLEMENTED WITH FREEZE-DRIED STRAWBERRY
POWDER

1 ! 1
CANSU TOPKAYA , FATMA IIK , FATMANUR BYKSARA

ABSTRACT
Strawberry fruits, namely Fragaria, is a member of the Rosacae family. The garden strawberry
or modern commercial strawberry is also known as Fragariaananassa. Strawberries are popularly
consumed not only in fresh and frozen forms but also as processed and derived products, including
yogurts, beverages, jams, and jellies. Regarding their nutritional and phytochemical composition,
strawberries are extremely rich fruit in bioactive compounds such as vitamins A, E, and C and dietary
sources of phytochemicals, mainly represented by phenolic compounds. Strawberries have immune
regulator, blood-sugar regulator, anti-inflammatory and anticarcinogenic effects thanks to their
phenolic content. Fresh consumption of these fruits provides a significant benefit to health. Different
drying methods can be used in order to provide longer shelf life for fresh fruits and vegetables. Freeze-
drying is one of the best methods to dry fresh vulnerable fruits by protecting their nutrient value.
Demand of processed foods, having functional property at least one, is increasing with urbanization.
Bakery products have longer shelf life than other fresh foods, easy available and have many different
sorts like cakes, breads, biscuits, crackers etc., including other basics protein such as milk, eggs, flour,
for human body. Besides these products are so improvable with some other foods or compounds to
obtain functional food. Since ancient times biscuit is one of the basic bakery products that is widely-
consumed by many people, among all ages, all over the world because wheat flour is one of the easy
reachable materials to cook or to consume. Furthermore, biscuits have low humidity that is reason why
has long shelf-life so it has a special place in human diet as snack food. Basic ingredients of biscuits
are wheat flour, sugar, margarine, water or milk. It may contain trace amount salt, baking powder or
any other ingredients depending on recipe or according to taste. The objective of this study to
determine some organoleptic properties of biscuits which were fortified with freeze-dried strawberry.
Biscuits were produced by replacing freeze-dried strawberry powder with wheat flour. Substitution
rates of freeze-dried strawberry 4%, 8% and 12%. Color of biscuits were determined via
HunterlabMiniScan XE. L (lightness) and b (yellowness) value were decreased by increasing ratio of
freeze-dried strawberry powder. However, a (redness) value increased with ratio of freeze-dried
strawberry powder. Color, smell, flavor, crunchiness and overall acceptance were evaluated by
sensory panels. Sensory panel was done with participation of 32 people. The biscuits having 4%
strawberry was more liked in terms of color and crispiness while control samples were more liked in
terms of smell, taste and overall acceptance. The biscuits having 4% strawberry powder in the
formulation were more liked than other added-biscuits when only added-biscuits were evaluated
among themselves, in all criteria.

Corresponding Author: CANSU TOPKAYA, ctopkaya08@hotmail.com


1
PAMUKKALE NVERSTES

1568
KEYWORDS
Strawberry, biscuit, functional food, sensory analysis

1569
Poster Session 12
Submission ID: 1548

DETERMINATION OF LAURUS NOBILIS L. FROM HATAY REGION


BY TRIPLE QUAD GC/MS

1 2 3
MUSTAFA KEMAL SANGN , SEVGIN ZDERIN , GRAY KILINEKER

ABSTRACT
Laurus nobilis L. is from the family of Lauraceae, which comprises numerous aromatic and
medicinal plants (Hogg et al., 1974). Laurus nobilis L. is also known as sweet bay, bay laurel, Grecian
laurel, true bay, and bay. The dried leaves are generally used in cooking, and the essential oil is used in
the flavourings industry (Bauer and Garbe, 1985). Laurel essential oil, also called laurel leaf oil or
sweet bay essential oil, is also used for the preparation of hair lotion due to its antidandruff activity
and for the external treatment of psoriasis. Laurus nobilis L. fruits are generally utilized for the
production of perfumed soaps and candle manufacture because of their fatty acid content (Hafizoglu
and Reunanen, 1993). The essential oil of leaves has antibacterial, antimicrobial properties and a
potential natural agent for breast cancer therapy. (Ozcan and Erkmen, 2001, ). Different studies made
on the essential oil show influence of the area of culture, of variety and harvest season on the chemical
composition (Flamini et al., 2007). 1.8-Cineole has been identified as the major component of many
plant essential oil as well as Laurus nobilis L. (Sangun et al., 2007). Laurus nobilis L. leaves were
harvested in October 2015 respectively from Antakya, Hatay region. Air-dried leaves were related to
water distillation for 4 hr using a Clevenger-type apparatus to produce the essential oils. The oils were
dried over anhydrous CaCl2 and stored in sealed vials at low temperature in refrigerator before
analysis. The essential oils were analysed by triple quad GC-MS using Agilent (7000 series) and
compared with GC-MS Hewlett Packard GCD (model 6890) and (model 5972) equipped with a mass
selective detector (MSD). In this study, the composition of the essential oil from the leaves has high
content of 1.8-Cineole, Sabinene and -Terpinyl acetate, but a low content of -Pinene, -
Phellandrene and trans--osimen. 1.8-Cineole was found major component of the leaves essential oil
obtained from Laurus nobilis L.

KEYWORDS
Laurus nobilis L., Essential oil, Triple Quad GC/MS, Antakya-Hatay, Turkey

Corresponding Author: MUSTAFA KEMAL SANGN, ksangun@gmail.com


1
MUSTAFA KEMAL UNVERSTY, FACULTY OF SCENCE & ARTS, CHEMSTRY DEPARTMENT, 31024,
HATAY.
2
MULA SITKI KOMAN UNVERSTY, TRF APPLCATON AND RESEARCH CENTER, MULA.
3
UKUROVA UNVERSTY, FACULTY OF SCENCE&ARTS, CHEMSTRY DEPARTMENT, 01330, ADANA.

1570
Poster Session 12
Submission ID: 1549

PLACE OF MEDICAL AND AROMATIC PLANTS ORGANIC


AGRICULTURE

1 2 ! 1 1
HLYA SAYI , NURGL TREM , MEHTAP BAYKAL , AYHAN SAYI , CELAL ERBA

ABSTRACT
Production is the process of shaping nature in order to meet people's needs. Natural resources
used unconsciously in eliminating the needs in this process caused irreparable devastation in the
balance of nature. As in all areas, the agricultural production process, in which all of its activities are
carried out in accordance with the nature, has suffered great damage to nature. In this process, negative
impacts on human and environmental health of chemical plant protection inputs used unconsciously in
agriculture to increase production led to the search for new plant protection products alternative to
these inputs. Medicinal and aromatic plants, which find use in the food and cosmetic industry in a
multifaceted way in coordination with the medical and pharmaceutical industries, also have an
important potential in combating diseases and pests. Pyrethrum, Artemisia, Mentha, Thymus, Salvia,
Origanum, Ocimum, Urtica, Melissa essential oils and extracts are harmful to organic farming with
herbal medicines such as azadirachtin, nicotine, pyrethrum, rotenone, allethrin, sabadilla and ryania
and some medicinal and aromatic plant species. Can be used against the fight against. Allelopathic
effects of secondary metabolites produced by medicinal and aromatic plants in weed control can also
be utilized. However, repellent, deterrent and antifeedant properties of these secondary compounds are
now seen as an advantage for organic farming. It is stated that plants belonging to the family
Lamiaceae, which contain plants with economical prescriptions such as lavender, mint, sage, thyme,
reyhan, are the plants most commonly used for plant disease pest and weed control. The major
producers of lavender in the world are England and France. France's Provenal region accounts for
60% of its production. A few years ago, the disease that took place in lavender fields reduced
production to half. Bulgarian producers have increased their production at this point, and Bulgaria is
known as the 'new lavender sovereignty'. In recent years, the effects of synthetic pesticides used in our
country and in the world have come to the fore to negative effects on human beings, people and
animals, and alternative methods for combating agricultural pests have begun to be preferred.
Considering the developments in organic farming in particular, chemical products based on vegetable
origin which are friendly to the environment have been preferred instead of chemical products. These
can be in various forms, such as unprocessed plant materials, plant extracts, and pure compounds
isolated from plants. Lavender plantation is being done intensely in and around Isparta province in
Turkey, but it can not meet the needs of the country. It has been observed that root leaves, hulls and
extracts of lavender are preventing germination and seedling formation of some weeds. In addition,
medicinal and aromatic plants are potential plants that can be taken into alternative sowing seasons,
and the extracts and essential oils obtained from these plants can be used to fight against some insects.
In the long run, this great mistake was made when most of the people began to suffer from the
consequences of this destruction. Now a new and vital constraint has emerged. This is the natural

Corresponding Author: HLYA SAYI, husaygi@gmail.com


1
UKUROVA NVERSTES YUMURTALIK MESLEK YKSEKOKULU
2
UKUROVA NVERSTES ZRAAT FAKLTES BAHE BTKLER

1571
balance that must be observed in the production process. The solution to this important problem is the
sustainable production philosophy based on the understanding that production can be done by
protecting nature.

KEYWORDS
Medical plants, Organic agriculture, Lavender, Allelopathy, Fields of application

1572
Poster Session 12
Submission ID: 1550

THE IMPORTANT MEDICAL PLANTS CONSUMED AS FOOD IN THE


CENTRAL ANATOLIAN REGION

1
ASUMAN KAN

ABSTRACT
Human being have been using the medicinal plants grown in the environment in food,
medicine, feed, fuel and handicrafts since they exist. The most important place among these plants is
the medicinal plants. Information on the use of medicinal plants for food and medicine is transmitted
from generations to generations. Our country is one of the richest countries in the world in terms of its
diversity of medicinal plants due to its geographical position and climate conditions. It is known that
the Central Anatolia region in Turkey is the region with the most endemic plant species with about
50%. The collected of various edible medicinal plants from Anatolia and their consumption as food
plays an important role in the healthy life cycle of rural areas. Edible plants also have endemic medical
plants. The medicinal plants consumed by the people of Central Anatolia as food within the nutritional
system based on carbohydrates such as oat, wheat, and tarhana which are mainly cereals constitute an
important place. Important medicinal plants that can be cooked and renewed in the Central Anatolian
Region are Malva sylvestris, Rumex patientia, Urtica dioica , Polygonum cognatum, Ferula
elaeochytris and Asparagus acutifolius. The phenolic compounds contained in these plants are rich in
protein, A, C and E vitamins, nutritional fibers, mineral substances and essential fatty acids, thus
increasing their potential for use as vegetables in our daily diet. In the face of the nutritional problems
that arise in our country, it is necessary to transfer the generations of the medicinal plants, which are
extremely important in terms of healthy and balanced nutrition consumed for food, and to protect them
as cultural identity.

KEYWORDS
Central Anatolia, Edible Medicinal Plants, Food, Diet

Corresponding Author: ASUMAN KAN, asumankan42@gmail.com


1
SELUK NVERSTES, TEKNK BLMLER MES. YK. OKULU, GIDA LEME BLM

1573
Poster Session 12
Submission ID: 1552

FUNCTIONAL PROPERTIES OF RICE BRAN

1 1
RACIYE MERAL , BURCU ERTRK

ABSTRACT

D
Rice (Oryza sativa) is one of the most important cereal product in the developing world
especially Asian countries and it is the staple food of over half the world's population. It is grown in
more than 100 countries and there are around 18,000 varieties accounting for about 25% of the

TE
worlds food grain production. About 610 million metric tons of rice are produced annually in recent
years. More than half of the production belongs to Asian countries such as China, India, Indonesia,
Bangladesh, Vietnam, and Thailand. One of the major by-products is rice bran which is accounted for
8% of milled rice. Rice bran is a waste product in the milling process and obtained when it is removed
EN
from the starchy endosperm in the rice milling process. Rice bran is a source of proteins, oil, nutrients,
and calories.It is high in oil content (1525%), has a low moisture content (67%) and possesses a
powdery consistency. it has been used as a feedstock and has the potential to be used as a food
ingredient and oil source. Rice bran, which was earlier used primarily as animal feed, is now finding
major application in the form of rice bran oil. India and Thailand have been the most successful
ES
countries in rice bran oil production. Rice bran has several unique properties. One such feature is the
presence of significant levels of minor-elements such as oryzanol, tocotrienol and phytosterols that
have a large nutraceutical application. It is a rich natural source of vitamin E, containing up to 300
mg/kg. The major components of vitamin E in rice bran are, -tocopherol, and -tocotrienol. Rice bran
PR

also contains about 3000 mg/kg -oryzanol. The studies confirm that rice bran has more than 20%
dietary fibre and it is also an excellent source of protein, minerals, unsaturated fat and vitamins. Rice
bran protein is higher in lysine content than rice endosperm protein and other cereal bran proteins.
Rice bran has great potential in food applications, especially in development of functional foods. They
are used in the production of healthy products. Several studies reported the minor components of the
rice bran such as gamma oryzanol, phytosterols and other phytosterol conjugates have antioxidant
T

property against the free radicals. Rice bran contains large concentrations of several compounds and
has the potential to prevent a range of chronic diseases. It is believed that rice bran serves as an
O

important functional food that has cholesterol lowering properties, cardiovascular health benefits and
anti-tumor activity. The gamma oryzanol of rice bran reduced a prominent amount of elevated serum
levels in hypothyroid patients. Rice bran fractions prevent high blood pressure, hyperlipidemia, and
N

hyperglycemia.

KEYWORDS
Rice, rice bran, oryzanol

Corresponding Author: RACYE MERAL, raciyemeral@yyu.edu.tr


1
YZNC YIL NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1574
Poster Session 12
Submission ID: 1553

ANTIOXIDANT ACTIVITY AND PHENOLIC PROFILES OF GRAPE


SEED

1 1
RACIYE MERAL , BURCU ERTRK

D
ABSTRACT
Grape (Vitis vinifera) is one of the world's largest fruit crops, which approximates an annual

TE
production of 58 million metric tons. Grapes (Vitis vinifera) have been used for their medicinal and
nutritional value for thousands of years. Grape seed extract has an incredible antioxidant potential with
its flavonoid phytonutrients. These polyphenols include resveratrol and unique oligomeric
proanthocyanidin complexes. Grape seeds are a rich source of (+)-catechins, ()-epicatechin and ()-
epicatechin-3-O-gallate, and dimeric, trimeric and tetrameric procyanidin. There are antimutagenic
EN
and antiviral agents of these compounds and these compounds prevent cardiovascular diseases. Today,
standardized extracts of grape seed may be used to treat a range of health problems related to free
radical damage, including heart disease, diabetes, and cancer. Recognition of such health benefits of
catechins and procyanidins has led to the use of grape seed extract as a dietary supplement. Studies
ES
have been reported of the procyanidin composition of grape seeds. Grape seed extract may help with a
type of poor circulation and high cholesterol. Grape seed extract also reduces swelling caused by
injury and helps with eye disease related to diabetes.Many people are interested in grape seed extract
because it contains antioxidants.There are several studies reported grape seed may be alternative to
synthetic antioxidants. It was determined grape seed promote oxidative stability of cooked meats and
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fish filets. Acccording to another study the addition of grape seed improved the rheological properties
by increasing dough development time and stability of the dough. The results obtained from this study
indicate that grape seed can be successfully used in the bread formulae both to improve rheological
properties of the dough and to increase antioxidant activity of the bread. In this study, antioxidant
activity, total phenolic content (TPC) and phenolic composition of grape seed were determined.
Antioxidant activity was determined by DPPH free radical-scavenging assay and TEAC assay.
T

Vitamin C and BHT were used as positive control. DPPH scavenging activity of grape seed was 93 %
at a concentration of 50 mg/mL. This value is comparable with vitamin C and higher than BHT.
O

TEAC value was calculated as 490 mol mol Trolox. TPC was determined using the Folin-Ciocalteu
method and results were expressed as gallic acid equivalent (GAE) in mg/100 g material. The result of
N

the analysis showed that grape seed has phenolic content of 87 mg/100 GAE. HPLC analysis
demonstrated grape seed included 9.4 mg/100g gallic acid, 160 mg/100g catechin, 2.20 mg/100g
ferulic acid, 4.60 mg/ 100g rutin, 2.30 mg/100g o-coumaric acid, 1.20 mg/100g p-coumaric acid, 0.80
mg/ 100 g quercetin.

KEYWORDS
Grape seed, antioxidant activity, pheolics

Corresponding Author: RACYE MERAL, raciyemeral@yyu.edu.tr


1
YZNC YIL NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1575
Poster Session 12
Submission ID: 1554

EFFECT OF SINAPIC ACID ON SOME METASTASIS GENES IN


LNCAP HUMAN PROSTATE CANCER CELL LINE

1 ! ! 1
CANAN EROLU , ERCAN KURAR , EBRU AVCI , HASIBE VURAL

ABSTRACT
Prostate cancer is also an important disease in terms of chemopreventive strategies due to late
age of onset, slow progression, high incidence, identified preneoplastic lesions, and risk groups. A
result of the studies demonstrating that synthetic antioxidants can be toxic and carcinogenic, public
interest in fruits, vegetables, spices and herbal drugs that are sources of natural antioxidants has
increased. Phenolic compounds found in foods are usually identified as inhibitors and potential
antioxidants of harmful oxidative processes related to cancer and anti-inflammatory diseases. Sinapic
acid (3,5-dimethoxy-4-hydroxycinnamic acid), is a phenolic compound and found in various
vegetables (potato and artichoke etc.) and fruit (apples, cherries, strawberries and plums etc.) species,
is a derivative of hydroxycinnamic acid. The aim of the study was to investigate the anti-metastatic
effect of sinapic acid, in the LNCaP human prostate cancer cells. Cytotoxic effect of sinapic acid was
determined by using XTT assay. Total RNA isolation of control and dose groups (IC50 dose of sinapic
acid) was conducted using TRIzol Reagent. Expressions of important genes in metastasis including
MMP2, MMP9, TIMP1, TIMP2, CDH1 and CDH2 were investigated in the control and the dose
groups by qPCR. IC50 dose of sinapic acid was detected as 1 mM for 72h in LNCaP cells. According
to qPCR results, significant decreases in the expressions of CDH2, MMP2 and MMP9 genes were
determined as 3.23, 3.25 and 2.92 folds, respectively, compared with the control group cells. It is
thought that sinapic acid demonstrates antimetastatic activity by regulating expression of important
genes in metastasis on LNCaP cells. Furthermore, more detailed studies should be conduct to
illuminate molecular mechanism of antimetastatic activity of sinapic acid on prostate cancer.
Financially supported by N.E.U. Scientific Research Projects (BAP #151218023).

KEYWORDS
LNCaP cells, Metastasis, Sinapic acid.

Corresponding Author: CANAN EROLU, cananeroglu88@gmail.com


1
NECMETTN ERBAKAN UNVERSTY

1576
Poster Session 12
Submission ID: 1555

COMPOSITIONS AND HEALTH BENEFITS OF POMEGRANATE


PRODUCTS

1 2
EMINE ERDA , VILDAN UYLAER

ABSTRACT
Pomegranate is a fruit that use for traditional treatment methods among society where is
homeland Asia minor. It is also known since ancient times and including in many sacred book. As it
consumed fresh, also used anyway like pomegranate juice, pomegranate juice concentrate, as color an
flavoring agent in various nutrient; by proccessing the wine, liquer and vinegar; as dehydrated , which
fruit is produced widely in Turkey. Some studies are reported that pomegranate pruducts change in
some blood value ( like HDL, LDL and cholestrol ), prevent the prostate cancer, be effective with
Alzheimer and increase sperm quality in male patients. Especially phenolic component content of
pomegranate products are very effective in preventing the occurrence some disease, causing premature
deaths, like cancer, vein and hearth disorders. This article provided information on the content of
pomegranate and pomegranates products and its utility to human health.

KEYWORDS
Pomegranate, pomegranate juice, pomegranate products, antioxidant, polyphenol

Corresponding Author: EMNE ERDA, emineerdg@gmail.com


1
NECMETTN ERBAKAN NVERSTES
2
ULUDA NVERSTES

1577
Poster Session 12
Submission ID: 1558

NIGELLA SATIVA AND ITS EFFECT ON THE HEALTH

1 ! ! 1
MEKIYE NC , BRA BEKAR , EZGI TOPTA BIYIKLI , ALI EMRAH BIYIKLI

ABSTRACT
Nigella Sativa,known one of three species of the genus Nigella from the plant family
Ranuncula, is one of frequently consumed plants and has a rich historical past. It generally grows in
West Asia, Middle East and Europe in the world and around Konya in Turkey. It has been used as
protective and flavoring for long years. The active components of the plant take place in the seeds of
Nigella sativa. In the recent years, in both our country and Middle East, it has become one of the
popular spices of alternative medicine. Since it is believed that it showed positive effects on health in
Middle East countries, it is also known as blessed seed. Thymoquinone, among active components of
Nigella sativa, is important in terms of its effects on health.. AIM:. With this study, it was aimed to
compile the studies examining the effects of Nigella sativa on health RESULTS: Some in vivo and in
vitro studies carried out suggest that the active components in the seed of Nigella sativa showed
effectivity inhibiting tumor formation. In the studies examining the effect of Thymoquinone, one of
the active components of Nigella sativa on cancer cultures, in similar way, was showed that the growth
of cancerous cell was reduced. But, there are also some studies showing that a significant effect was
not provided. In a number studies carried out, it was seen that the seed of Nigella sativa provided
positive effect on immune system and it was met its inhibitor effect on allergy formation and
inflammation process. In a number of similar studies, it was claimed that the use of fat of Nigella
sativa on allergic diseases such as allergic rhinitis, bronchial asthma, and atopic asthma may have
positive effects. In addition, it was reported that it may make contribution to controlling hypertension,
providing diuretic effect. In the studies examining its antibacterial effect, it was revealed that it
frequently showed protective effect against many species of bacteria such as .coli, Bacillus subtilis,
Streptococcus faecalis, Staphylococcus. In a number of studies carried out on the individuals with
Type 2 diabetics, it was identified that Thymoquinone among the components of Nigella sativa
significantly reduced HbA1C and also with antioxidative effect, its protective effect against neuronal
damage was met. But there are some studies suggesting that it did not show positive effects on
diabetics. Although Anticonvulsant effects of use of Epilepside Thymoquinone are met, there are no
numerous supportive studies. CONCLUSION: Current literature data related to the Nigella sativa
concentrated on antioxidative, antibacterial, anti-inflammatory, anticonvulsant, immune system
supportive, and hyper glycaemia reductive effects and its positive effects were frequently met. But the
studies carried out are frequently based on the administration of Nigella sativa as supplement, the
amount of daily consumption is not clear. Any adverse effect regarding its use in high doses were not
reported. But since its long term effects are not completely known, consumption in high quantity
should be avoided and comprehensive studies on the subject should be carried out. Keywords: Nigella
sativa, health, thymoquinone

Corresponding Author: MEKYE NC, Dyt.mekiyeoncu@hotmail.com


1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEKOKULU BESLENME VE DYETETK
BLM

1578
KEYWORDS
Keywords: Nigella sativa, diet, health, thymoquinone

1579
Poster Session 12
Submission ID: 1560

ELLAGIC ACID

1 1
FATMA GNL SOLMAZ , ERKAN YALIN

ABSTRACT
ELLAGIC ACID Fatma Gnl SOLMAZ, Erkan YALIN Ondokuz Mays University,
Faculty of Arts and Sciences, Department of Biology, 55139 Samsun, Turkey E-mail:
gonul.solmaz@omu.edu.tr ABSTRACT Ellagic Acid (EA, 2,3,7,8-tetrahidroksi-chromeno; C14H6O8)
has been characterized as a natural dimeric derivative of gallic acid. It is found in various fruit and
vegetables including strawberry, mulberry, pomegranate and walnut. Ellagic Acid, which is a
phenological plant compound, has been studied extensively in various experimental cancer models.
Besides a decrease in the incidence of chemically induced lung, small intestine, breast and esophagus
tumors, decreases have been observed in rat esophagus tumors, chemically induced mutagenesis and
tumor diversity. Ellagic Acid can be observed in different tissue and cells of mammals, such as liver,
endothelial cells, immune cells, kidney and lung and it is also an anti-inflammatory agent and
antioxidant. As a result of the assessment of the effects of ellagic acid on rat C6 glioma cell cultures
when two dimensional culture models are used, it was found that ellagic acid decreased tumor cell
viability, caused impairment in cell structure and inhibited cell proliferation. Ellagic Acid also heals
oxidative damage following renal ischemia/reperfusion damage and histopathological changes that
occur. In another study conducted in 2006, it was found that in patients who drank a glass of (200-250
ml) pomegranate a day following radiotherapy or surgery, the doubling period of PSA increased to 54
weeks from 15 weeks. Pomegranate juice contains polyphenols, gallotannins and anthocyanin and it
has been reported to prevent prostate cancer in vitro. In our country, compounds, which are obtained
from flowers, leaves, shells or other parts of various plants through different methods, are used in the
treatment of almost all kinds of diseases from cancer to diabetes. For a molecule to be a drug, first of
all there should be a hypothesis which predicts it can be useful in a specific disease or a symptom.
According to the results of all these studies, ellagic acid is a healing compound with antioxidant
effects and it prevents cell proliferation. With these properties, it is in line to become a drug. Aspirin,
which is well known today (active ingredient, acetylsalicylic acid) was discovered with the
observation that shells of willow tree reduced fever. Accordingly, fruit such as pomegranate, mulberry,
raspberry and strawberry, which are distributed in our country with a rich flora, should be assessed
effectively in terms of ellagic acid active ingredient. Ethnobotany studies should be stepped up in our
country. Biologists, pharmacologists and pharmacists should assess the results obtained and their
contributions to both the field of health and to the economy of the county should be increased by
making the necessary attempts about the subject.

KEYWORDS
ellagic acid, pomegranate

Corresponding Author: FATMA GNL SOLMAZ, gonul.solmaz@omu.edu.tr


1
ONDOKUZ MAYIS NVERSTES

1580
Poster Session 12
Submission ID: 1563

MERSN ( MYRTUS COMMUNS L. ) HERBS

1
FSUN TEMAMOULLARI

ABSTRACT
Myrtle (Myrtus communis L) is a medicinal and aromatic plant from the Myrtaceae family. It
is found in coastal regions of our country whereas it is often grown in Mediterranean countries, Asia,
America and in other countries such as Southern Russia, Iran, Tunisia and New Zealand in the world.
The plant, which has got evergreen leaves, is a chick pea-sized, blackish bush with purple seeds.
Myrtle is consisted of rich volatile oils, phenolic compounds, flavonoids, tannins, anthocyanin
pigments and fatty acids. The plant has antiseptic, deodorant, constipating, appetising, sedative,
antihemorrhagic, antioxidant, antimicrobial, antifungal, antiinflammatory, antimutagenic, insecticidal,
antinociceptive, antiviral, analgesic, molluscicidal and antigenotoxic effects as well as antiprotozoal
properties against Trichomonas vaginalis and antiparasitic properties against Leishmania and
Plasmodium. M. communis fruit was used in Traditional Medicine in the treatment of many different
infectious diseases such as diarrhea and dysentery, and its leaves were benefited as an antiseptic and
antiinflammatory agent in the treatments of urinary infections, wound healing and antifungal diseases
(Candidiasis) as well as an antidiabetic or an antitussive agent and were used in the management of
stomachache. Although its rare incidence, toxicity of myrtles might lead nausea, vomiting and
diarrhea; besides respiratory distress and asthma attacks in infants and children. In this review the
traditional use of myrtle plant will be informed along the pharmacolog

KEYWORDS
Myrtle, Pharmacology, Toxicology, traditional use

Corresponding Author: FSUN TEMAMOULLARI, farmafto@gmail.com


1
HARRAN NVERSTES VETERNER FAKLTES, FARMAKOLOJ VE TOKSKOLOJ A. D. ANLIURFA

1581
Poster Session 12
Submission ID: 1564

USAGE OF LINSEED FOR FUNCTIONAL MILK PRODUCTION

1 1 2
SAKNE YALIN , MUHAMMAD SHAZAB RAMAY , SUZAN YALIN

ABSTRACT
In recent years consumer demands concerning food have changed significantly and concept of
functional food has been growing extensively. Functional foods are modified foods for certain
physiological functions other than providing nutrient requirements. Milk fat consists mostly of
saturated fatty acids resulting in negative consumer perception due to health concern related to
saturated fats. Thus, there is an opportunity to alter the milk fat content and its fatty acid composition
according to the consumer needs. It is very difficult to modify milk fatty acid composition for human
health. Linseed contains about 36-48% oil rich in unsaturated fatty acids. Alpfa linolenic acid
constitute of approximately 50-55% of total fatty acids in linseed. Linseed is an important feedstuffs
that can be used as an energy and protein source in lactating dairy cattle. It has high antioxidant
activity due to high content of secoisolariciresinl diglucoyside as a precursor of lignans in linseed.
Dietary linseed supplementation can increase the content of alpha-linolenic acid and conjugated
linoleic acid, decrease the ratio of omega 6/omega 3 and saturated fat content and increase the
proportion of stearic and relative to other saturated fatty acids. These enhancements in the fat profile
of milk give consumers food with a healtier fat profile.

KEYWORDS
Linseed, Milk, Functional milk, Fatty acid

Corresponding Author: SAKNE YALIN, sayalcin@ankara.edu.tr


1
ANKARA UNVERSTY, FACULTY OF VETERNARY MEDCNE, DEPARTMENT OF ANMAL NUTRTON,
ANKARA, TURKEY
2
SELUK UNVERSTY, FACULTY OF VETERNARY MEDCNE, DEPARTMENT OF FOOD HYGENE AND
TECHNOLOGY, KONYA, TURKEY

1582
Poster Session 12
Submission ID: 1565

DETERMINATION OF PHENOLIC CONTENTS OF CYCLAMEN


ALPINUM BY HPLC

1 1
MURAT TURAN , RAMAZAN MAMMADOV

D
ABSTRACT
Cyclamen genus belonging to the family Primulaceae, is used as an ornamental plant as well

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as a medicinal plant. Antifungal, antiinflammatory and antinociceptive activity properties have been
determined in studies and are also used in folk medicine. Cyclamen alpinum Dammann ex. Sprenger
was identified by Schwarz as endemic under the name Cyclamen trochopteranthum O. Schwarz in
1975 but nowadays although it is again called C. alpinum, its endemism is controversial. Phenolic
compounds and quantities were determined by HPLC from C. alpinum. 9 standard phenolic
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compounds were performed and the amounts indicated in C. alpinum plant were determined; gallic
acid (10.01 g/g), 3,4 dihydroxy benzoic acid (33.65 g/g), 4 hydroxy benzoic acid (125.33 g/g),
chlorogenic acid (2.73 g/g), vanillic acid (121.35 g/g), caffeic acid (0.13 g/g), p-coumaric acid
(5.78 g/g), ferulic acid (3.25 g/g), cinnamic acid (2.25 g/g). The amount of phenolic substances at
ES
the end of the HPLC may vary according to the method of extraction of the plant and the conditions of
the region it is growing.

KEYWORDS
PR

Cyclamen alpinum, HPLC, Phenolic content


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O
N

Corresponding Author: RAMAZAN MAMMADOV, rmammad@yahoo.com


1
PAMUKKALE UNVERSTY

1583
Poster Session 12
Submission ID: 1566

HOPS (HUMULUS LUPULUS)

1 2
IDEM EB EN , FSUN TEMAMOULLARI

ABSTRACT
Humulus lupulus, also known as "Maya Herb" or "Beer Flower", is widely grown around in
Bilecik-Pazaryeri, Turkey. Hops plant is a plant with a stout root, heart-shaped leaves and cone-like
flowers. It is an indispensable raw material of beer production since the desired bitterness, aroma and
taste is provided with hops depending on the beer. In recent years, hops, which have begun to be used
in pharmaceuticals and cosmetics as well as beer making, are a multi-year industry plant. Hops
preparations are used as sedatives, especially for the treatment of sleep disorders, and for activating
gastric functions. It has also antibacterial and antifungal effects. A potent phytoestrogen in hops, 8-
prenylnaringenin (8-PN), has an activity greater than other established plant estrogens. Although some
researchers have reported no estrogenic effect of the hops, other researchers have found a high
estrogenic effect. In another study conducted, the prenyl flavonoids obtained from the hops were
investigated effect on cancer, menopausal temperature pressures and osteoporosis, and as a result they
were found to slow down the bone erection and reduce the temperature pressures. It has also been
suggested that these compounds may be responsible for a variety of reproductive disorders, including
a decline in sperm concentration in men with negatif effects on sexual desire. In this review, the effect
of hops on sexual activity was investigated.

KEYWORDS
Hops, sexual activity

Corresponding Author: FSUN TEMAMOULLARI, farmafo@gmail.com


1
HARRAN NVERSTES, VETERNER FAKLTES, DLERME VE SUN TOHUMLAMA A. D., ANLIURFA
2
HARRAN NVERSTES, VETERNER FAKLTES, FARMAKOLOJ VE TOKSKOLOJ A. D., ANLIURFA

1584
Poster Session 12
Submission ID: 1567

THE USE OF AROMATIC PLANTS, ESSENTIAL OILS, AND THEIR


ACTIVE COMPONENTS IN BROILER NUTRITION AS PHYTOGENIC
FEED ADDITIVES

1 ! 1
FGEN KIRKPINAR , ZGN IIK , SELM MERT

ABSTRACT
In recent years, products containing essential oils derived from several spices and herbs could
be used feed additives as growth promoters in animal nutrition. The ban on the use of antibiotics as
feed additives has accelerated the investigation of alternative feed additives for animal nutrition
especially broiler nutrition. Herbs, spices and products derived from them are mainly essential oils.
Great amounts of these active components can also be found in essential oils (EO) of the associated
aromatic plants. Hence, the chemical composition and concentration of active components varies
greatly dependent on their source. These phytogenic additivites may have more than one mode of
action, including improving feed intake and flavour, stimulating the secretion of digestive enzymes,
increasing gastric and intestinal motility, endocrine stimulation, antimicrobial, anti-viral,
anthelminthic and coccidiostat activities, immune stimulation, anti-inflammatory and anti-oxidative
activity and pigments. Natural feed additives of aromatic plant origin, also referred to as phytogenic
substances are healthier, less regarded as chemical hazards and generally regarded as safe. Aromatic
plants, essential oils, and their active components are incorporated in broiler diets to replace synthetic
feed additives in order to stimulate or promote the effective use of feed nutrients which may
subsequently result in better growth performance and improved feed efficiency. Moreover, active
components of aromatic plants may improve digestion and stimulate the immune function in broilers.
Therefore, the purpose of this review is to give an overview on and definition of aromatic plants their
EO and active components as phytogenic feed additives, chemical composition and mode of action, as
well as on the use of these ingredients in broiler diets with particular attention paid to broiler
performance characteristics.

KEYWORDS
Aromatic plants, essential oils, broiler

Corresponding Author: FGEN KIRKPINAR, figen.kirkpinar@ege.edu.tr


1
EGE NVERSTES

1585
Poster Session 12
Submission ID: 1568

EFFECTS OF IRRADIATION AND MODIFIED ATMOSPHERE


PACKAGING ON THE QUALITY OF THYME DURING STORAGE

1 2
CELALE KIRKIN , GRBZ GNE

ABSTRACT
The aim of this study was to evaluate the effects of gamma-irradiation and modified
atmosphere packaging on the quality properties of thyme during storage. Dried and ground thyme
(Thymus vulgaris) was packaged under 100% N2 (modified atmosphere packaging, MAP) or air
(aerobic packaging, AP), and gamma-irradiated at 0 kGy (as control), 6 kGy or 14 kGy. The changes
in total mesophilic aerobic bacteria and yeast-mold counts, sensory properties (odor, color intensity,
overall acceptability), essential oil yield, total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl
(DPPH) radical scavenging activity and antimicrobial activity were evaluated during 6-month storage.
Total mesophilic aerobic bacteria and yeast-mold counts of thyme were approximately 4.7 log/cfu
(MAP) and 4.5 log/cfu/g (AP) in control and decreased to below 1 log/cfu after irradiation at 6 and 14
kGy at the beginning and did not change during storage. The color intensity of 14 kGy irradiated
thyme in MAP was lower compared to the control at the beginning of the storage, but the difference
was not significant after 6-month storage. Irradiation under MAP increased essential oil yield and
DDPH radical scavenging activity at time zero; however, the differences due to irradiation were lost
after 6 months of storage. Color intensity and overall acceptability of the spice were decreased after 6-
month storage. There was an increase in total phenolic content in AP, but a reduction in DPPH radical
scavenging activity in the essential oil of thyme during 6-month storage. An increase in antimicrobial
activity against Bacillus cereus was noted after 14 kGy irradiation at the beginning of the storage
period. The antimicrobial activity against Escherichia coli and Staphylococcus aureus was higher in
MAP than AP. The antimicrobial activity of the thyme essential oil was decreased during 6-month
storage. In conclusion, Irradiation under both types of packaging was highly effective on
decontaminating thyme. Irradiation under MAP had a positive contribution to essential oil yield,
antioxidant and antimicrobial activity of thyme, but a negative effect on color intensity compared to
irradiation under AP. The effects of MAP on the irradiation-induced changes were generally become
insignificant after 6-month storage.

KEYWORDS
thyme, irradiation, modified atmosphere packaging, quality

Corresponding Author: CELALE KIRKIN, celale.kirkin@ozyegin.edu.tr


1
OZYEGN UNVERSTY
2
ISTANBUL TECHNCAL UNVERSTY

1586
Poster Session 12
Submission ID: 1569

ONOSMA MALATYANA BINZET EXTRACT MEDIATED


BIOSYNTHSIS OF AG DOPED ZNO NANOPARTICLES AND ITS
SENSING APPLICATION

1 ! 1 1 1 1
ERSAN TURUNC , RIZA BNZET , HAKAN ARSLAN , OSKAY KAHRAMAN , GUN BNZET , LKAY GUMUS , EBRU
1 1 1 1
KESKN , UMMUHAN SOLMAZ , BIRDAL ARSLAN , ALI NAIL SELCK

ABSTRACT
The fabrication of nanostructured materials have gained much attention in the field of catalysis
in recent years by virtue of their unique properties, large surface area, and strong interaction with other
materials. Semiconductor-based heterostructures play an important role in nanodevices and sensors
applications. ZnO nanostructures are technologically important material and are used in a wide range
of applications such as catalysis, photocatalysis, sensors and other industrial applications [1-4].
Similiar to other semiconductors, ZnO has poor selectivity or sensor response. ZnO is also doped with
nobel metals (Ag, Au and Pt) to achive selectivty and enhance sensor response. In this study, we report
a facile, simple and low cost synthesis of Ag doped ZnO nanoparticles using Onosma malatyana
Binzet root extract. The synthesized Ag doped ZnO nanoparticles were detailed characterized by
scanning electron microscopy, X-ray powder diffractometer, UV-vis spectroscopy and dynamic light
scattering. In addition we fabricated Ag-ZnO modified carbon paste electrode (AgZnO-CPE) for
detection of paracetamol (Figure 1). Figure 1. Typical cyclic voltammogram of bare CPE, ZnO-CPE,
Ag-CPE and AgZnO-CPE electrode with 0.1 mM paracetamol in 0.1 M phosphate buffer solution (pH
= 7.20) at scan rate 50 mV/s. Inset show magnification of bare CPE and ZnO-CPE response.
Keywords: Green synthesis, Onosma malatyana, nanoparticle, sensor, paracetamol
Acknowledgements This study was supported by the Research Fund of Mersin University in Turkey
with Project Number: 2016-1-AP4-1429. References [1] K. Saoud, R. Alsoubaihi, N. Bensalah, T.
Bora, M. Bertino, J. Dutta, Synthesis of supported silver nano-spheres on zinc oxide nanorods for
visible light photocatalytic applications Materials Research Bulletin 63 (2015) 134-140. [2] B. Sarma,
B. K. Sarma, Fabrication of Ag/ZnO heterostructure and the role of surface coverage of ZnO
microrods by Ag nanoparticles on the photophysical and photocatalytic properties of the metal-
semiconductor system Applied Surface Science 410 (2017) 557-565. [3] R. Kumar, D. Rana, A. Umar,
P. Sharma, S. Chauhan, M. S. Chauhan, Ag-doped ZnO nanoellipsoids: Potential scaffold for
photocatalytic and sensing applications Talanta 137 (2015) 204-213. [4] Y. Wang, X. He, K. Wang, X.
Zhang,W. Tan, Barbated Skullcup herb extract-mediated biosynthesis of gold nanoparticles and its
primary application in electrochemistry Colloids and Surfaces B: Biointerfaces 73 (2009) 75-79.

KEYWORDS
Green synthesis, Onosma malatyana, nanoparticle, sensor, paracetamol

Corresponding Author: ERSAN TURUNC, ersanturunc@mersin.edu.tr


1
MERSN UNVERSTY

1587
Poster Session 12
Submission ID: 1571

EFFECTS OF DIETARY OREGANO OIL ON NUTRITIONAL


QUALITY OF BROILER MEAT

1 ! 1
FGEN KIRKPINAR , SELM MERT , ZGN IIK

ABSTRACT
The general consumer rejection to synthetic additives has been increasing in recently. For this
reason, in recent years interest has arisen in the use of natural essential oils with the intention to
improve meat quality, without leaving residues in the meat or the environment. Broiler meat has many
desirable nutritional characteristic such as low lipid content and relatively high concentrations of
polyunsaturated fatty acids (PUFA) such as n-3 PUFAs [eicosapentaenoic acid (EPA) and
docosahexaenoic acid (DHA)]. Physicochemical and sensory properties of broiler meat as well as its
storage life is very important. The addition of oregano essential oil in broiler diets could effective in
delaying lipid oxidation. The better oxidative stability of broiler meat receiving the diets supplemented
with oregano oil may probably the result of antioxidant constituents of the oregano oil that entered the
circulatory system and were distributed and retained in meat. Furthermore, the strong smell of oregano
oil may penetrate into muscles and organs, which would improve their composition, colour, pH, lipid
oxidation and sensory properties as well as storage and processing values. The essential oil of oregano
mainly consists of carvacrol, thymol, and their precursors, c-terpinene and q-cymene it has already
been used with the intention to improve the quality and quantity of broiler meat. Furthermore, oregano
oil improves meat storage stability after slaughter in poultry, protects against the negative effects of
stress on broiler meat quality characteristics. Thus, the addition of oregano oil to broiler diets could
significantly positive affect chicken meat quality. The purpose of this review is to provide an overview
of the published data on the potential of oregano oils in broiler meat productin and to describe their
possible modes of action with particular attention paid to carcase characteristics, meat composition,
colour, pH and sensory quality of broiler meat.

KEYWORDS
Oregano oil, broiler meat characteristics

Corresponding Author: FGEN KIRKPINAR, figen.kirkpinar@ege.edu.tr


1
EGE NVERSTES

1588
Poster Session 12
Submission ID: 1572

IMPORTANCE OF BLACK SEED (NIGELLA SATIVA) ON LOW-


CHOLESTEROL EGG PRODUCTION

1 2 3
SAKNE YALIN , LYAS ONBAILAR , S. SONGL YALIN

ABSTRACT
Egg as one of the animal products is an important food source. Eggs that highly consumed in
the World contain many essential amino acids, fatty acids, vitamins and minerals for healthy nutrition.
Egg consumption has been reported to decrease the blood glycemic index and increase the high-
density lipoprotein cholesterol level. However the most important factors for not reaching an adequate
level of egg consumption are consumption habits, egg cholesterol level and factors leading to
arteriosclerosis and coronary heart failure of high cholesterol foods. To prevent coronary heart
diseases daily cholesterol consumption is limited to 300 mg according to the reports of National
Cholesterol Education Program and American Heart Associations Nutrition Committee. Egg
consumption decreases in the developed countries due to the cholesterol level of 200-300 mg in an
egg. Because of those reasons, low cholesterol egg production is of great importance for public health.
For this purpose dietary supplementation of additives especially probiotics, various plant and plant
extracts has been used. Black seed (Nigella sativa) from these natural additives has been widely used
in treaditional for respiratory health, gastrointestinal health, kidney and liver functions, circulation and
immune system. Egg yolk cholesterol concentration decreased with dietary black seed. This reduction
may be due to a decrease in the concentration of triacylglycerol and phospholipid in the serum. Black
seed plays an important role in functional egg production by reducing cholesterol synthesis by
inhibiting the lipogenic pathway of acetyl CoA in the liver.

KEYWORDS
Black seed, low-cholesterol egg, functional food

Corresponding Author: SAKNE YALIN, sayalcin@ankara.edu.tr


1
ANKARA UNVERSTY, FACULTY OF VETERNARY MEDCNE, DEPARTMENT OF ANMAL NUTRTON,
ANKARA, TURKEY
2
HACETTEPE UNVERSTY,TRANSGENC ANMAL TECHNOLOGES RESEARCH AND APPLCATON CENTER,
ANKARA, TURKEY
3
HACETTEPE UNVERSTY, FACULTY OF MEDCNE, DEPARTMENT OF PEDATRCS, ANKARA, TURKEY

1589
Poster Session 12
Submission ID: 1573

GNSENG AND CANCER

1 1 1 1
ESRA CAN , SALHA NUR ERYT , EZG TOPTA BIYIKLI , AL EMRAH BIYIKLI

ABSTRACT
GINSENG AND CANCER The concept health affecting lifetime and quality of life is defined
as wellbeing of the individual from the physical, emotional, and social aspect. The factors such as
changing life conditions, unhealthy diet, sedentary life time, and stress, negatively affecting health,
prepare a ground for the various diseases. On the prevalence of cancer, which shows rapidly increase
in the present days, the effect of unhealthy diet is highly important. With understanding of importance
of diet, the interests of people have increased to the various nutrients and supplements to protect their
health. One of these plants is also ginseng. Ginseng makes effect on health with its components such
as ginsenoside, triterpenic saponosides, polysaccharides, peptides, polyacetylenic alcohols, and fatty
acid. In the recent period, particularly on the diseases such as cancer and diabetics, the studies toward
the effects of ginseng have increased. AIM:. In this study, it was aimed to compile the relationship
between ginseng intake and sort of cancer. METHOD: In the study, the effect mechanism of ginseng
in being protected from cancer were scrutinized. By examining the actual literature data studying the
relationship of ginseng consumption and cancer, the effects of ginseng on cancer formation and course
of cancer formed were evaluated in detail. RESULTS Just as there are a number of studies showing
that ginseng has a protective effect against cancer, there are also a lot of publications showing that it is
not effective. It was suggested that ginseng, with saponosides and ginsenocides, which present in its
content, showed anticarcinogenic effect against the various sort of cancer. However, its molecular
mechanism is not clear. Thanks to ginsenocides, the studies were met, which showed that it reduced
cytokines and provided an effect strengthening immune system in cancer patients. It was reported that
ginseng may be effective on the various sorts of cancers such as lung and liver cancers, particularly
prostate and ovarian cancers on women. In addition, also in liver fibrosis with it antioxidative feature
and by supplying fat destruction, its effects toward reducing fat accumulation were seen. The amounts
of ginseng used in the studies differ from each other and the suggestions regarding the amount of use
are not clear. CONCLUSION:. It was seen that ginseng may be effective on the different sorts of
cancers. It was identified that it had positive effects on especially prostate, ovarian, and lung cancers.
However, some studies were also met, which showed that it was not effective on cancer. The studies
on the use of ginseng for cancer treatment were less and it is stated that its effect mechanism is not
clear. There is a need for further studies on this subject. In addition, for being able to offer clear
suggestions about the amount of use, the number of studies should be increased. Keywords: Ginseng,
cancer, diet, antioxidant

KEYWORDS
GINSENG,CANCER,DIET,ANTIOKXIDANT

Corresponding Author: ESRA CAN, esra_621@hotmail.com


1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEKOKULU BESLENME VE DYETETK
BLM

1590
Poster Session 12
Submission ID: 1574

INVESTIGATION OF LINDEN (TILIA SPP.) AND ROSEHIP (ROSA


CANINA) SAMPLES SOLD IN TEKRDA PROVINCE IN TERMS OF
AFLATOXINS

1 2
NURAY CAN , SERAP DURAKLI VELIOLU

ABSTRACT
Linden (Tilia spp.) and rosehip (Rosa canina) are widely consumed as alternative herbal tea by
adults and children in order to benefit from healthy components they contain or prevent various
diseases. However, studies have shown that herbal tea including linden and rosehip can be
contaminated with toxigenic molds as well as pathogenic bacteria, and under favorable conditions
molds can produce mycotoxins which have negative effects on human health. The presence of
aflatoxins, known to be carcinogenic, teratogenic, mutagenic and hepatotoxic, and classified as "Group
I: Carcinogenic to humans" by the International Agency for Research on Cancer (IARC), in these
products is a threat to public health. However, the number of studies on the presence of aflatoxins in
linden and rosehip is limited. For this reason, the aim of this study was to investigate the presence of
aflatoxins in these products using HPLC method. 15 linden and 15 rosehip samples sold as
unpackaged were used as the material of the study. The samples were provided from Tekirda
province in Turkey in February and March 2015. In addition to aflatoxin content, moisture and water
activity values and total mesophilic aerobic bacteria and total yeast-mold counts were also determined.
0.158 g/kg AFG1 and 0.168 g/kg AFG2 were determined in one of the linden samples and 0.162
g/kg AFG2 was determined in another linden sample. Aflatoxin levels of these samples were not
higher than the maximum permissible levels. The rest of the linden and rosehip samples were
determined to be contaminated with at least one of the four aflatoxins with the levels below the limit
of quantification (0.155; 0.168; 0.156; 0.162 g/kg for AFB1, AFB2, AFG1, AFG2, respectively). The
mean moisture content of linden and rosehip samples were found to be 10.97% and 14.58%,
respectively. The mean water activity values were determined as 0.58 and 0.62 for linden and rosehip
samples, respectively. Total mesophilic aerobic bacteria counts of the linden samples ranged between
<1.0x103-4.3x106 cfu/g and the rosehip samples ranged between <1,0x103-6,4x104 cfu/g. Total
yeast-mould counts of the linden samples ranged between <1,0x103-1,2x105 cfu/g and the rosehip
samples ranged between <1,0x103-3,6x105 cfu/g. As a result, yeast-mold and mesophilic aerobic
bacteria contamination were detected in linden and rosehip samples, and aflatoxin was detected at low
levels. This results suggest that toxigenic molds can grow in linden and rosehip and under sufficient
conditions aflatoxins can be produced in these products. Acknowledgment The authors thank to
Namk Kemal University Scientific Research Projects Commitee for the support of this study having
the project number NKUBAP.00.24.YL.14.18.

KEYWORDS

Corresponding Author: SERAP DURAKLI VELOLU, svelioglu@nku.edu.tr


1
STANBUL AYDIN NVERSTES
2
NAMIK KEMAL NVERSTES GIDA MHENDSL BLM

1591
Linden (Tilia spp.), rosehip (Rosa canina), aflatoxins

1592
Poster Session 12
Submission ID: 1575

CURRENT SITUATION, MARKETING STRUCTURE AND PROBLEMS


OF HERBALISTS AND PROCESSING FIRMS OF MEDICINAL AND
AROMATIC PLANTS: THE CASE OF MERSIN PROVINCE, TURKEY

1 1 1
ARZU SECER , GIZEM YENER , ZOZAN KAYA

ABSTRACT
Medicinal and aromatic plants play a valuable and important role in economic, social, cultural
and ecological aspects of local communities the world over. Consumer demand has been increased in
recently since people has been preferred to use less synthetic medicines and tended to solve their
health problems with natural ways. Turkey has great opportunity to supply medicinal and aromatic
plants because of its advantages in genetic diversity, climate, geographic location, rich plant flora and
wide agricultural area. In Turkey, medicinal and aromatic plants are collected from mainly Eagan,
Marmara and Mediterranean Regions. In terms of biodiversity, Mersin is one of the richest provinces
in the Mediterranean Region of Turkey. Recently, many plants have produced and set up operations on
getting essential oils in this province. In this study, it is aimed to reveal current situation, marketing
structure and problems of herbalists and processing firms in medicinal and aromatic plants sector in
Mersin. Research area is defined as Mezitli, Yeniehir, Akdeniz ve Toros which are central provinces
of Mersin. The main material of the study was primary data gathered by face-to-face questionnaires
with 65 herbalists and in-depth interviews with 7 processing firms. Collected data was presented as
frequencies, ratios and averages. Also SWOT analysis was applied to identify sectors strengths and
weaknesses and identify opportunities and the threats which the sector can face to the sector.
According to findings, herbalists generally provided their products from wholesalers and sold to
consumers directly. For herbalist, the most important problems are inadequate organization in the
sector, knowledge pollution on medicinal and aromatic plants and inadequate information about the
sector in public. Whereas, processing operations prefer to provide their raw materials from producers,
other firms and collectors. These operations expressed that inadequacy of raw materials, lack of
qualified staff and inadequacy of production capacity were the most important problems in the sector.

KEYWORDS
Medicinal and aromatic plants, marketing structure, SWOT, Mersin, Turkey.

Corresponding Author: ARZU SECER, asecer@cu.edu.tr


1
CUKUROVA UNVERSTY

1593
Poster Session 12
Submission ID: 1576

EFFECTS OF CITRONELLOL, GERANIOL AND NEROL ON


VIOLACEIN PRODUCTION

1 1
HALIME EVKBA , SEYHAN ULUSOY

ABSTRACT
Quorum sensing (QS) is a cell to cell communication system. Bacteria co-ordinate the gene
expression of many bacterial behaviors including violacein, antibiotic, virulence factors production
and biofilm formation using this system. Violacein is a purple pigment by produced Chromobacterium
violaceum with controlled QS. In this study, inhibitory effects of citronellol, geraniol, nerol were
tested on the production of violacein using Chromobacterium violaceum CV026 and C. violaceum
VIR07 biomonitor strains. The results showed that citronellol, geraniol and nerol inhibited violacein
production by 75%. Therefore, citronellol, geraniol and, nerol might be suitable for development into
antivirulence agents.

KEYWORDS
Citronellol, geraniol, nerol, violacein

Corresponding Author: HALME EVKBA, halimecevikbas@gmail.com


1
SLEYMAN DEMREL NVERSTES

1594
Poster Session 12
Submission ID: 1577

THE EFFECT OF CORNELIAN CHERRY ON THE RISK OF CANCER

1 ! 1
ESRA CAN , AL EMRAH BIYIKLI , EZG TOPTA BIYIKLI

ABSTRACT
THE EFFECT OF CORNELIAN CHERRY ON THE RISK OF CANCER In sustaining a
healthy life, the role of adequate and balanced diet is relatively more. In the recent year, one of the
factors kept responsible for the increase of the prevalence of cancer is also unhealthy diet. It is known
for long years that providing adequate and balanced diet and give more place to antioxidative nutrients
in diet had a protective effect against cancer. It was suggested that cornelian cherry, one of the
antioxidative nutrients, with the strong phenolic substances in its content, could be effective against
cancer. AIM:. In this study, it was aimed to evaluate the relationship between cornelian cherry
consumption and risk of cancer. METHOD: In this compilation study, by examining the actual
literature data studying the relationship of cornelian cherry consumption and cancer, the effects of the
fruit cornelian cherry toward the risk of cancer were evaluated in detail. RESULTS: It is known that
the antioxidative effect of cornelian cherry arises from the phenolic substances in its content. It is
reported that, cornelian cherry, preventing free radical formation, is protective against cancer. In
addition, with components of flavonoids and amino acid, affecting lipid mechanism, it supports its
antioxidative effect. In a number of studies, it was observed that the fruit cornelian cherry provided a
protective effect against a number of sort of cancer at the significant level, particularly prostate cancer,
breast, uterine, and liver cancers. In the individuals consuming high amount of cornelian cherry, the
risk of cancer formation was found to be significantly lower compared to the individuals consuming
less cornelian cherry. But there are also studies identifying that cornelian cherry consumption showed
no effect on cancer. It is stated that giving place to cornelian cherry in daily diet may be protective
against cancer and the amount suggested to the consumers are not clear in the studies.
CONCLUSION:. It was seen that cornelian cherry, an important antioxidant, can provide protective
effects against cancer by means of the different mechanisms. Its positive effect in being protected from
prostate cancer is remarkable. However, the effect of the fruit cornelian cherry on the studies toward
using it in cancer treatment is not clear and there is a need for further studies on this subject. It is
suggested that its consumption should be increased and the number of the studies on the amount of
effective intake should be increased.

KEYWORDS
Cornelian cherry, cancer, antioxidant, , phytology, flavonoids

Corresponding Author: ESRA CAN, esra_621@hotmail.com


1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEKOKULU BESLENME VE DYETETK
BLM

1595
Poster Session 12
Submission ID: 1580

AN ANALYSIS OF THE ANTIMICROBIAL ACTIVITY OF CASTANEA,


RHODODENDRON AND ASTRAGALUS HONEYS

1 2 1
DUYGU ODABA ALVER , MER ERTRK , SEVIM FT YEGN

ABSTRACT
This study aims to compare the antimicrobial activities of different honeys collected from
different regions. Honey is a sweet substance that honeybees store in honeycombs after collecting
flowers nectars and secretion of plants and certain living beings on the plants, and converting them by
adding their unique substances. Honey consists of around 400 components. Honey has been used for
medical purposes by numerous cultures since the antiquity. In this study, pollen analyses, which are
compliant with the literature, were conducted on the total of 9 honey samples collected from different
places, and it was detected that 3 of them were Castanea (Castanea sativa), other 3 were
Rhododendron (Rhododendron sp.) and the last three were Astragalus (Astragalus sp.). The pH, %
Brix values and antimicrobial activities were studied. Antimicrobial activities of honey samples were
investigated by using disc diffusion assay method against four Gram positive bacterias (B. subtillis, S.
aureus, L. monocytogenes, C. perfringens), four Gram negative bacterias (P. aeruginosa, E. coli, S.
enteritidis, K. pneumoniae) and a fungus (C. albicans) also. pH and % Brix values were measured by
pH meter and refractometer. It was established that the lowest pH value was those of G1 and G2
honey samples as 3,62, the highest pH value was detected as 4,55 on K3 sample. The lowest %Brix
values was in K2 sample as 55,76 and the highest was 78,1 in R3 sample. The highest antimicrobial
activity among the samples was demonstrated by K3 sample against L.monocytogenes (13.77 mm),
while the lowest average antimicrobial activity was performed by R1 sample (8,41 mm). R2, R3, K1,
K2 and K3 samples showed antifungal activity on Candida type fungi, while no antifungal activity was
observed in R1, G1, G2 and G3 samples. The highest antifungal activity was seen on R3 sample
(11.79 mm). The pH and %Brix values observed in the study have similar properties with those of
previous studies. Also, the honey samples have been observed to have similar antimicrobial activities.

KEYWORDS
Honey, Antimicrobial activity, pH, Brix

Corresponding Author: DUYGU ODABA ALVER, duygu.odabas@giresun.edu.tr


1
GRESUN NVERSTES
2
ORDU NVERSTES

1596
Poster Session 12
Submission ID: 1582

THREATENED STATUS OF MEDICINAL PLANTS COLLECTED


FROM NATURE IN TURKEY

1 2 1 3
ELIF BOZDOAN SERT , GLBIN ETINKALE DEMIRKAN , SEMA GLER , GLDEN SANDAL ERZURUMLU ,
4
ZERRIN ST

ABSTRACT
Association between people and native plants are constituted from traditional uses and
colleting from nature in Turkey where is in a rich region with a total of 12 000 plant taxa. Many of the
plant species take their place in different forms in markets depending on the regional or local
resources. Number of plants on the list which are obtained in different studies on this subject are also
high. Collected part of 500 taxa in this group also known as non-wood forest product is also different
each other. Damage level may change depending on the collected organs and collecting density. For
instance, collected flower and leaf parts from linden species (Tilia sp.) doesnt cause a significant
damage to the plant. However, collected some plants together with roots will always constitute a
danger for these plants. Regular collecting of species that are widespread in Turkey and have high
market value slow down the process of extinction. This situation can change depending on the ability
of regeneration, rate of reproduction. According to IUCN data, 15 000 medicinal plants in worldwide
are at risk in different categories. Approximately 350 plant species traded by collected from nature in
Turkey, and 35 of these plants are endemic. Among the most important reasons in damage of
medicinal plants are change in land use except for the colletion process. In this study, threats of
species that are traded by collecting from nature in Turkey are revealed; some insights have been made
on this subject by taking into consideration to distribution areas. For instance, Liquidambar orientalis
is not yet reported as endangered, but its just on the verge of danger. While the area of this plants
distribution in 1949 is more than 6 000 hectares; it is now below 1 500 hectares. Its also seen that
Juglans regia is in danger at near threatened status; while Cedrus libani is also at vulnerable status.
Incase these species are formed with large groups in certain areas such as valley or forests, are in
danger of extinction. The danger level may increase in the shrubs or herbaceous plants that are spread
in smaller areas or open into different uses. For instance, Tchihatchewia isatidea native endemic in
East Anatolian Region is within the vulnerable status.

KEYWORDS
Medicinal plants, colleting from nature, status of threatened, Turkey

Corresponding Author: ELF BOZDOAN SERT, ebozdogan@mku.edu.tr


1
MUSTAFA KEMAL UNVERSTY, FACULTY OF ARCHTECTURE, DEPT. OF LANDSCAPE ARCHTECTURE
2
MULA SITKI KOMAN UNVERSTY, VOCATONAL SCHOOL OF ORTACA
3
MER HALSDEMR UNVERSTY, FACULTY OF ARCHTECTURE, DEPT. OF LANDSCAPE ARCHTECTURE
4
UKUROVA UNVERSTY, FACULTY OF AGRCULTURE, DEPT. OF LANDSCAPE ARCHTECTURE

1597
Poster Session 12
Submission ID: 1583

DIETARY THYME (THYMUS VULGARIS L.) USAGE TO IMPROVE


SHELF LIFE OF POULTRY MEAT

1 2 3
SUZAN YALIN , SAKNE YALIN , LYAS ONBAILAR

ABSTRACT
Poultry meat has an important food among animal food sources. Meat is an important food for
human health due to having protein, essential amino acids, lipids, minerals and vitamins. Poultry meat
has many desirable nutritional properties such as low lipid content and relatively high concentration of
polyunsaturated fatty acids. Increasing the degree of unsaturation of meat by dietary manipulation
increases susceptibility of meat lipids to oxidative deterioration. Lipid oxidation affects colour, odour
and flavor negatively and a reduced shelf life. In recent years researchers have focused on increasing
shelf life of meat. For this reason plant and plant extracts having antioxidant activity have been used
extensively. Thyme (Thymus vulgaris L.) has been studied by several researchers. Thyme oil contains
phenolic compounds such as thymol, carvacrol and -terpinene having important biological activities
and pharmacological properties. Thyme can improve the oxidative stability of meat due to having
these phenolic compounds. Considering the consumer preference for natural antioxidants, thyme could
be used as natural antioxidant for improving shelf life of meat.

KEYWORDS
Thyme, Poultry meat, Antioxidant, Shelf life

Corresponding Author: SUZAN YALIN, syalcin@selcuk.edu.tr


1
SELUK UNVERSTY, FACULTY OF VETERNARY MEDCNE, DEPARTMENT OF FOOD HYGENE AND
TECHNOLOGY, KONYA, TURKEY
2
ANKARA UNVERSTY, FACULTY OF VETERNARY MEDCNE, DEPARTMENT OF ANMAL NUTRTON,
ANKARA, TURKEY
3
HACETTEPE UNVERSTY,TRANSGENC ANMAL TECHNOLOGES RESEARCH AND APPLCATON CENTER,
ANKARA, TURKEY

1598
Poster Session 12
Submission ID: 1586

ANTIMICROBIAL ACTIVITY OF SOME SPICES

1
ENOL KSE

ABSTRACT

D
In this study, antimicrobial activities of methanol extracts of some packaged and unpackaged
spices including thyme, cumin, mint, cinnamon were investigated against Escherichiacoli ATCC
11303, Pseudomonas aeruginosa ATCC 19429, Salmonella typhimurium ATCC 13311, Enterococcus

TE
faecalis ATCC 33186 and Bacillus cereus ATCC 11778. For determination of antimicrobial activity,
different concentrations of these spice extracts were tested against bacterias by agar diffusion method.
It was examined that the extracts of unpackaged spices exhibited higher antibacterial activity against
Enterococcus faecalis ATCC 33186 and Bacillus cereus ATCC 11778 in comparison to packaged
EN
spices. Methanol extracts of all spices showed variable degrees of antibacterial activity against the
other bacterias. The Enterococcus faecalis ATCC 33186 was determined as the most resistant strain
for antimicrobial activity of packaged and unpackaged spices of methanol extracts. Also Ampicillin
(10g) and Tetracycline (30g) were used to determine sensitivity of the tested bacterias and
comparison.
ES

KEYWORDS
PR

Spices, antimicrobial activity, agar diffusion method


T
O
N

Corresponding Author: ENOL KSE, senolkose28@gmail.com


1
YUZUNCU YIL UNIVERSITY, ENGINEERING FACULTY, FOOD ENGINEERING DEPARTMENT

1599
Poster Session 12
Submission ID: 1587

PHYTOCHEMICAL AND PHARMACOLOGICAL PROPERTIES OF


SUMAC SEED

1 ! 1
MURAT KARAAA , SUZAN YALIN , YUSUF BER

ABSTRACT
Sumac (Rhus coriaria L.) seed has long been used as an important spice in traditional
medicine. Sumac is rich in B vitamins, gallic acid, benzoic acid, ascorbic acid, hydrolysable tannins,
volatile oils, anthocyanins and flavonoids. The major volatiles are aliphatic, aldehydes, hexahydro
farnesyl acetone and oxygenated terpenes. Due to the presence of these bioactive compounds sumac
has properties of antibacterial, antifungal, antioxidant, anti-inflammatory, anticancer, hypoglycemic
and hypolipidemic activities. Sumac is rich in oleic and linoleic acids. Myricetin is the major flavonol
in sumac seed. Water extracts of sumac seeds have a strong antioxidant and antimicrobial activity
against food-born pathogenic bacteria. Xanthones and aromatic components have been active
properties against Candida albicans and Aspergillus flavus. The sourness of sumac is due to the
presence of organic acids such as malic, citric and tartaric acids, whereas the astringent taste is due to
its tannin. Therefore sumac has an economic importance due to a source of functional food,
nutraceutical ingredients, its also increasing use in cosmetic and phaarmaceutical industries.

KEYWORDS
Sumac, Functional food, Phytochemical properties, Pharmacological Properties

Corresponding Author: SUZAN YALIN, syalcin@selcuk.edu.tr


1
SELUK UNVERSTY, FACULTY OF VETERNARY MEDCNE, DEPARTMENT OF FOOD HYGENE AND
TECHNOLOGY, KONYA, TURKEY

1600
Poster Session 12
Submission ID: 1588

IN VITRO ANTIBACTERIAL ACTIVITY OF ESSENTIAL OILS FROM


MEDICINAL PLANTS AGAINST BACTERIAL FISH PATHOGENS

1 1
ZNUR DILER , ZNUR GRMEZ

ABSTRACT
During the last decades, there has been a continuous growth of aquaculture industries all over
the world and such intensive production would experience disease problems. Various synthetic
chemicals and antibiotics have been used to prevent or treat fish diseases with a partial success.
However, continuous use of antibiotics leads to drug resistance and thereby to a reduced efficacy of
the drugs. In recent years, the ability of medicinal plants to antimicrobial effective has been studied.
The objectives of this study were: to investigate the in vitro antibacterial effect of Oregano (Origanum
vulgare), St. John's-wort (Hypericum perforatum) and Nettle (Urtica dioica) essential oils against
bacteria species (Yersinia ruckeri and Lactococcus garviae) by disc difusion test. The essential oils
except to U. dioica that was most antibacterial effect against Y. ruckeri and L. garvieae microbial
strains.

KEYWORDS
Origanum vulgare, Hypericum perforatum, Urtica dioica, antimicrobial activity

Corresponding Author: ZNUR GRMEZ, oznurgormez@sdu.edu.tr


1
SLEYMAN DEMREL NVERSTES ERDR SU RNLER FAKLTES

1601
Poster Session 12
Submission ID: 1589

INVESTIGATION OF RHEOLOGICAL BEHAVIOR AND PHYSICAL


PROPERTIES OF THYME ESSENTIAL OIL AT DIFFERENT
TEMPERATURES

1 1 2 1
FILIZ ER , DENIZ DNER , SERDAL SABANCI , DERYA TEZCAN

ABSTRACT
Essential oils usually economically important plant-sourced oils. They are widely used in food
industry because of their aromatic and antimicrobial effect. They also have great economic
importance. In food industry, thyme essential (TEO) oil is used as an antimicrobial agent and some
food product used as a flavouring agent. In this study, rheological properties of TEO was investigated
at different temperatures (20, 30, 40, 50 and 60), and the best model describing the
rheological characteristics of this oil was determined. In addition, the physical properties (optical
rotation, refractive index, solubility ratios (v/v) with 96% ethanol, and relative density) and color
analysis of TEO were evaluated at different temperatures (20 to 40 and 60). It was determined
that rheological characteristics of TEO were best described with Newtonian model. For the same shear
rate, shear stress values and viscosity values decreased as the temperature increased. It has been
identified that color values of the essential oils were different depending on the temperature. As
temperature values increased L* values decreased while a*, b* values increased. Physical property
results of TEO were given depending on temperature (20C, 40C and 60C) respectively, as follows;
optical rotation values were 64.630.21, 65.840.22 and 86.560.16, refractive index values were
1.490.0015, 1.476, 1.47530.0047, solubility ratios (v/v) with 96% ethanol were 6, 3.33 and 3.83,
relative density values were 0.9660.38, 0.9430.02 and 0.7130.01 g/cm3. It was obtained that
physical and rheological properties of TEO depends on temperature. Higher process temperatures
adversely affected physical and quality properties of TEO. Low temperature process conditions are
recommended to be applied to provide higher quality products including thyme essential oil. It is
thought that results of these study could contribute valuable information for industrial handling of
TEO especially in pumping lines and heating processes. Further study on the shelf life of TEO and its
use in innovative applications should be conducted. This study was financially supported by Scientific
and Technical Research Council of Turkey (TUBITAK, 2209-A Undergraduate Students National
Research Funding Program).

KEYWORDS
essential oil; thyme, viscosity; quality; density

Corresponding Author: SERDAL SABANCI, serdalsabanci@hotmail.com


1
EGE NVERSTES
2
MUNZUR NVERSTES

1602
Poster Session 12
Submission ID: 1590

FUNCTIONAL FOOD: QUINOA

1 1 1
MUSTAFA AKTURFAN , SUZAN YALIN , YUSUF BER

ABSTRACT
Quinoa (Chenopodium quinoa) seed has excellent nutritive value due to having high and good
quality protein and essential amino acids. Essential amino acids are higher in quinoa than wheat.
Lysine in quinoa is two times higher in that of wheat. It is a good source of energy and rich in mineral,
vitamin and bioactive compounds. Quinoa has bioactive compounds such as polyphenols, saponins,
flavonoids and phenolic acids. These bioactive compounds decrease blood cholesterol level, prevent
the development of cancer cells, eliminate toxins, improve immune system and prevent cardiovascular
disease. The kinoa, which has its own unique aroma, has attracted a lot of attention recently in terms
of its suitability for Turkish taste as it is preferred in world cuisines. Quinoa has a wide range of uses.
Quinoa seed flour can also be used in bread making alone or mixed with other cereal flours. Pilaf can
be made from rice with quinoa seed. Beer-like beverages and breakfast cereals can be produced from
quinoa seeds. Since it has high nutritional value, it is also used in baby food production.

KEYWORDS
Quinoa, functional food, bioactive compounds

Corresponding Author: MUSTAFA AKTURFAN, mstf4207@gmail.com


1
SELUK UNVERSTY, FACULTY OF VETERNARY MEDCNE, DEPARTMENT OF FOOD HYGENE AND
TECHNOLOGY, KONYA, TURKEY

1603
Poster Session 12
Submission ID: 1591

THE USE OF MEDICINAL AND AROMATIC PLANTS AS A


FUNCTIONAL FOOD COMPONENT IN MEAT PRODUCTS

1
AZIM MEK

D
ABSTRACT
Functional foods are defined as foods that are formed by the addition of bioactive ingredients

TE
obtained from fully natural sources, to the foods we consume. Functional foods should provide
additional benefits on human physiology and metabolic functions, as well as meeting the basic nutrient
needs of the body. Essential oils, powders and extracts obtained from medicinal and aromatic plants,
also conjugated linoleic acid, dietary fiber and probiotic microorganisms are widely used in meat
products as a functional food components. Powders, essential oils and extracts used in meat products
EN
are obtained from seeds, leaves, stems, fruits and roots of medicinal and aromatic plants such as
culinary herbs, spices, fruits and vegetables. Medicinal and aromatic plants have many functional
properties (antioxidant, antimicrobial etc.) in meat products due to their secondary metabolites such as
phenolic acids, flavonoids, alkaloids, terpenoids and tannins. In addition, medicinal and aromatic
ES
plants used in meat products improve sensory properties such as taste, odor and color. Different
solvents (ethanol, methanol, acetone etc.) and extraction methods (maceration, percolation, soxhlet,
microwave etc.) are used to obtain essential oils and extracts. It has been reported in literature studies
that antioxidant activity is more effective in extracts obtained from solvent extraction, whereas
antimicrobial activity is more effective in essential oils obtained from water extraction. Furthermore,
PR

medicinal and aromatic plants, and their powders, essential oils and extracts have the potential to
become new generation substances for human and animal nutrition and health. In this study,
information about functional properties of powders, essential oils and extracts obtained from
medicinal and aromatic plants used in meat products were given, and studies about on this subject
were compiled.
T

KEYWORDS
O

Meat products, medicinal and aromatic plants, functional properties


N

Corresponding Author: AZM MEK, simsekazim@gmail.com


1
SLEYMAN DEMREL NVERSTES, ERDR MESLEK YKSEKOKULU, GIDA LEME BLM

1604
Poster Session 12
Submission ID: 1592

ANTI-FUNGAL EFFECTS OF SOME MEDICINAL PLANT ESSENTIAL


OILS ON ARUM ITALICUM UNDER IN VITRO CONDITIONS

1 ! 2
HAVVA DNLER , HUSSEIN ABDULLAH AHMED AHMED , SERKAN URANBEY

ABSTRACT
Geophytes, which constitute a significant part of our biological diversity, have a considerable
potential in food, perfumery, pharmaceutical industry, and ornamental plant industry owing to their
rich secondary metabolites, starch contents and ostentatious leaves and flowers. It is extremely critical
to produce and export of the plant A.italicum free from other pathogens, particularly fungal diseases.
In this study, anti-fungal effect of some medicinal plant essential oils (sage, thyme, lemon,
peppermint, and cinnamon) which were added into shoot regeneration medium following the
sterilization procedure was examined by using shoot explants on tubers of Arum italicum. Explants
taken from Arum italicum plant were washed by keeping under tap water for 30 minutes. Tubers were
firstly kept in 95% ethanol for three minutes and then were exposed to sterilization process in 15%
commercial bleach for 10 minutes. Sterilized shoot tips were rinsed with sterile bidistilled water 3
times for 5 minutes in each. MS medium containing plant growth regulators (1-4 mg/L BAP and 0.25-
1 mg/L NAA) 2% sucrose, and 0.8% agar in different combinations of shoot regeneration hormones
was prepared, essential oils in rates of 0 (Control), 125, 250, and 500 ppm were added into MS
medium. Essential oils were dissolved by using 0.1 % Tween 20 and 10% n-hexane. Sterilized
explants were inoculated into MS medium. They were cultured under controlled conditions in 16/8
hour illumination period, at 241 oC for 4 weeks. As a result of the study, it was determined that
essential oils of thyme, cinnamon, and peppermint inhibited fungal contaminations (Aspergillus sp.,
Penicillium sp., and Fusarium sp.) occurring in MS medium compared to the control and increasing
concentrations had a fungicidal effect.

KEYWORDS
essential oil, Arum italicum, in vitro, MS, antifungal activity

Corresponding Author: HAVVA DNLER, havva.dinler@usak.edu.tr


1
UAK NVERSTES
2
ANKARA NVERSTES

1605
Poster Session 12
Submission ID: 1594

ANTIOXIDANT EFFECTS OF SOME AROMATIC PLANTS USED IN


MEAT AND MEAT PRODUCTS

1
EBRU BAYRAK

ABSTRACT
Lipid oxidation is one of the most important changes in meat and meat products that reduce
quality and limit shelf life. Lipid oxidation causes negative changes on sensory attributes such as
color, flavor, odor and nutritive value of the product and potentially toxic compounds. The oxidation
of lipid-containing foods can be delayed or slowed down by the use of antioxidants. The antioxidants
can be of synthetic or natural origin. Although synthetic antioxidants have been widely used in the
meat industry to inhibit lipid oxidation, the trend is to decrease their use because of the growing health
concerns among consumers about such chemical additives. In recent years, some aromatic plants have
been commonly used in meat and meat products due to their antioxidant effects. These plants, which
are used as natural antioxidants, are important in terms of phenolic compounds. The antioxidant
activity of phenolic compounds is due to their ability to scavenge free radicals, donate hydrogen atoms
or electron, or chelate metal ions. Tea, various spices (rosemary, thyme, sage, etc.), grape and kernel,
berries and citrus fruits have high phenolic content and can therefore be used as natural antioxidant in
meat products. In this review, antioxidant effects of some aromatic plants used in meat and meat
products were discussed.

KEYWORDS
Lipid oxidation, aromatic plants, natural antioxidants, meat and meat products

Corresponding Author: EBRU BAYRAK, ebayrak@selcuk.edu.tr


1
SELUK NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1606
Poster Session 12
Submission ID: 1595

ORTA ANADOLU BLGESI TIBBI ADAAYI (SALVIA OFFICINALIS


L.) ISLAHI ARATIRMALARI

1 1 2 1 3
REYHAN BAHTIYARCA BAGDAT , NURETTIN CINKAYA , KADRIYE YUKSEL , CIGDEM BOZDEMIR , EMEL CAKIR

ABSTRACT
Salvia officinalis, Lamiaceae, known as Dalmatian Sage or common sage has been gaining
popularity in food and drug industry wordwide, recently. Even the existence of many sage species on
the World, the genus officinalis has medicinal common use. Indigestion and inflammation disorders,
excessive sweating, including that associated with peri-menopause; relief of pressure spots that result
from the use of a prosthesis; and as a flavoring for foods are the main areas where the plant is mainly
consumed. Sage oil has also been employed as a fragrance in soaps and perfumes. Wide adaptability
and non-selective climatic requirements of the plats made it possible to receive high biomass, and
several harvests during the same plantation period. Present study was conducted in Ankara, during the
successive two growing seoson of 2014 and 2016. Thirty-three spontenaus hybride common sage lines
(Salvia officinalis) were evaluated regarding their biomass production and essential oil charecteristics
employing 4 different standard sage cultivars and lines. Following yield parameters were recorded as;
the plant height was ranged 50.3 to 97.5 cm, canopy diameter was 36.0 to 95.0 cm, fresh herb yield
was changed 59.9 to 593.4 g/per plant, drug herb yield was 12.6 to 183.9 g/per plant, drug leaf yield
was 16.1 to 74.5 g/per plant and the leaf ratio was around 53.42-67.01%. The essential oil components
of the lines were determined by GC-MS at Western Mediterranean Agricultural Research Institute,
Antalya. The essential oil ratio was changed between 0.88 and 2.42%. All the yield parameters of the
lines were found statistically significant. -thujone, 1.8 cineole, borneol, camphor, -thujone,
camphene and viridiflorol were the main components. More than ten lines had less than 20% of -
thujone. Phytophtora cryptogea was identified as devastating pathogen confirmed by molecular
characterization, besides some other Fusarium species.

KEYWORDS
Common sage (Salvia officinalis L.), plant heigth, canopy width, drug herb yield, drug leaf
yield, essential oil yield, -thujone, Phytophtora cryptogea

Corresponding Author: REYHAN BAHTYARCA BAGDAT, reyhanbagdat@yahoo.com


1
CENTRAL RESEARCH INSTTUTE FOR FELD CROPS
2
WESTERN MEDTERRANEAN AGRCULTURAL RESEARCH INSTTUTE
3
DRECTORATE OF PLANT PROTECTON CENTRAL RESEARCH INSTTUTE,

1607
Poster Session 12
Submission ID: 1598

OVERDOSE OF CINNAMON BARKS IS THE CAUSE OF POISONING


IN THE GEDIATRIC PATIENT: CASE REPORT

1 2 4
IDEM OLAK , ELIF ELIBOL , TRKAN DEMIR, BIJEN KIVAK

ABSTRACT
The use of herbal medicine has existed since the beginning of human history and interest in
alternative medicine has increased in the last two decades. The use of these methods has increased in
treatment. Also, drug interaction, toxicity, overdose and side effects are increasing. Approximately, 70
percent of the herbal drug users continue to use another drug at the same time. The fact that the use of
herbal remedies is usually unconsciously recommended by others and the herb is not mentioned in the
anamnesis when the herb is being used. Moreover, the doctor is not informed and plant-drug
interaction does not take place. Therefore, the prospectuses affects drug use negatively. There are
many physiological changes that may affect the herbal pharmacy and the pharmacokinetics of older
people. For example; the reduction of the gastrointestinal motility and the surface of the absorbation,
the reduction of total body water, the decrease in serum albumin depletion and protein binding, the
decrease in biotransformation, the decrease in renal blood flow drug abstinence, the decrease in
receptor sensitivity and the change in cellular response. Due to these factors, side effects due to herbal
treatment and more intoxications are observed in the elderly. Cinnamon, also known as "Darn",
"Lousa", "erbet Kokusu" among the people, is the dried shell of some Cinnamomum (Lauraceae)
species. There are two main varieties of cinnamon bark, namely Chinese Cinnamon (Cortex
Cinnamomi cassiae) and Ceylon Cinnamon (Cortex Cinnamomi zeylanici). Both cinnamon contains
tannin and 1-2% volatile oil in its composition. Both plants are grown in places like Japan, Ceylon,
South America, Sumatra. Cinnamon is anti-skidding, gas extractor and antiseptic properties (1,2).
Apart from that, it is also used as a spice and fragrance. The cinnamon oil obtained by distillation from
its shells has a pleasant smell and taste; and in the composition, cinnamic aldehyde and eugenol (3). It
has been used for thousands of years in terms of health. It is used in folk medicine with psychological
distress, cramenage, heart weakness, influenza, cold, flu, anorexia, indigestion, diarrhea, stomach
laxity, low body resistance and intestinal worms. The use of overdose is indicated to cause agitation,
hypoglycemia, tachycardia, to increase the level of the used coumarin and abortion, tachycardia and
arrhythmia in pregnancy. In this article, we present a case of emergency history of hypoglycemia,
agitation and tachycardia, history and clinical follow-up and a 76-year-old female patient admitted to
acute poisoning of cinnamon and honey mixture. It is our goal to present this case, it should be known
that with the recommendation of others and the use of an overdose of herbal medicines unconsciously
will cause intoxication, especially in the elderly and doctors need to consider the use of a medicinal
product in the etiology of causes such as tachycardia, agitation, hypoglycemia in patients with urgent
care. References: 1. Demirhan A., Msr ars Droglar, Sermet Matbaas, stanbul, 1975. 2.

Corresponding Author: DEM OLAK, cido_colak@hotmail.com


1
SB ZMR TEPECK ETM VE ARATIRMA HASTANES
2
ZMR GAZEMR NEVVAR SALH GREN DEVLET HASTANES
4
EGE NVERSTES ECZACILIK FAKLTES ECZACILIK MESLEK BLMLER BLM FARMAKOGNOZ
ANA BLM DAL

1608
Demirhan A. ifal Bitkiler, Doal lalarla Geleneksel Tedaviler, Alfa Yaynlar, stanbul, 2001. 3.
Piccioni A, Badellino E. Pre-natal (segment II) toxicity study of cinnamic aldehyde in the Sprague-
Dawley rat. Food Chem Toxicol. 1989 Dec;27(12):781-6 4. Miller KG, Poole CF, Chichila TMP.
Solvent-assisted supercritical fluid extraction for the iso- lation of semivolatile flavor compounds from
cinnamons of commerce and their separation by series-coupled column gas chromatography. J High
Resol Chromatogr 1995. 18: 461-471.

KEYWORDS
geriatric patient, cinnamon, hypoglycemia, tachycardia, agitation

1609
Poster Session 12
Submission ID: 1599

THE EFFECT OF ULTRASONIC PRETREATED MACERATION


PROCEDURE ON SOME PROPERTIES OF AROMATIZED OLIVE OIL
WITH THYME

1 ! ! 1 ! 1
KRAN KULEAAN , HATICE DEMIROK , HILAL BEDEL , SEDA ALDEMIR , AYE INAR , BURAK KSE ,
1
MERYEM CIHANGIR

ABSTRACT
In the study, natural extra virgin olive oil was aromatized with thyme by ultrasonic pretreated
maceration. It was aimed to the shortening of the duration of maceration by ultrasonic pretreated
application. Since mass transfer is faster and better during maceration in ultrasonic pretreated samples,
a more intense in flavor and volatile components of aromatized product was obtained in the end of
maceration period. The maceration procedures were carried out for 30 days in amber colored glass
bottles with and without ultrasonic pretreatment with equal amounts of oil and thyme. The ultrasonic
pretreated maceration process was performed in an ultrasonic bath at 20% power (253 W, 40 kHz) for
5 min and 10 min. At the end of the sonication procedure, the ambient temperature of the water bath
was measured as 27 C with an increase of 3 C. The percentage of free fatty acids and peroxide value
analysis was performed on the samples taken from ultrasound treated and non treated groups at 15th
and 30th days. Alterations in the amounts of volatile components of aromatized oils (p-cymene,
thymol and carvacrol) were determined by GC-MS. The percentage of free fatty acids of virgin olive
oil was determined as 0.5% and the peroxide value was 14 meqO2/kg in pre-maceration process. In all
applications, the percentage of free fatty acids and peroxide values were not changed after 30 days of
maceration period. The amount of p-cymene, thymol and carvacrol in the aromatized oil which
macerated for 30 days and were not subjected to ultrasound, were found as 2.06 (g/g), 391.26 (g/g)
and 585.28 (g/g) respectively. The highest values for these components were found as 2.66 (g/g),
614.18 (g/g) and 1044.06 (g/g) respectively in the maceration group which was ultrasonicated for
10 mins. The amounts of p-cymene, thymol and carvacrol of the olive oils which were ultrasonic
pretreated and subjected to maceration under the same conditions were higher as much as 1.3, 1.6, and
1.8 times respectively when their amounts compared to that of untreated ones.

KEYWORDS
Olive oil, thyme, ultrasound, maceration, ultrasonic.

Corresponding Author: KRAN KULEAAN, skuleasan@gmail.com


1
MEHMET AKF ERSOY NVERSTES MHENDSLK-MMARLIK FAKLTES GIDA MHENDSL
BLM, BURDUR

1610
Poster Session 12
Submission ID: 1600

IS ALGAE OIL ALTERNATIVE TO FISH OIL?

1 1
FERIDE AYYILDIZ , MERVE SEYDA KARACIL ERMUMCU

ABSTRACT
Algae are usually found in damp places or bodies of water. Microalgae are the primary
sources of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) for zooplankton, fish. It was
known that EPA and DHA in fish oil have a lot of positive effects on human health such as
cardiovascular diseases ,hypertension, insulin sensivity, neurological diseases etc. Recently algae oil is
a new field for using as an alternative to fish oil. Several photosynthetic (Nannochloropsis sp.
Hibberd, Phaeodactylum Bohlin, Nitzschia Hassall, Porphyridium Ngeli etc) and heterotrophic
(Schizochytrium sp., Ulkenia sp., Crypthecodinium Cohni etc) marine microalgae are considered as a
good source of omega-3 fatty acids (EPA and DHA) for production of algae oil. While photosynthetic
microalgae is usually used for production of EPA rich algae oil, heterotrophic microalgae is usually
used for production of DHA rich algae oil. A lot of studies indicated that DHA and EPA from fish oils
and algal sources provide health benefits to human. Algal-based supplements has led to a large
industry effort towards developing alternatives to fish oil. In addition algae oil is very good alternative
for vegeterian who intakes low essential fatty acids. But the health benefits of algae oil are uncertain
according to natural fish oils. There is a limited understanding of nutritional composition across algal
species. It is needed more studies about dose of algae oil to examine the protective effects of human
health. References Topuz OK. Algal ol: a novel source of omega-3 fatty acds for human nutrton.
Scientific Bulletin. Series F. Biotechnologies, Vol. XX, 2016. Cottin SC, Sanders TA, Hall WL .The
differential effects of EPA and DHA on cardiovascular risk factors. Proc Nutr Soc, 2011. 70:215231
Wells ML, Potin P, Craigie JS et al. Algae as nutritional and functional food sources: revisiting our
understanding. J Appl Phycol, 2016. Rossoll D, Bermudez R, Hauss H et al. Ocean acidification-
induced food quality deterioration constrains trophic transfer. PLoS One,2012. 7(4):e34737 ezanka
T, Petrnkov M, Cepk V, et al. Trachydiscus minutus, a new biotechnological source of
eicosapentaenoic acid. Folia Microbiol, 2010. 55:265269

KEYWORDS
Algae oil, omega 3, fish oil, EPA, DHA

Corresponding Author: FERDE AYYILDIZ, feridecelebi_dyt@hotmail.com


1
GAZ UNVERSTY FACULTY OF HEALTH SCENCES DEPARTMENT OF NUTRTON AND DETETCS

1611
Poster Session 12
Submission ID: 1601

ANTIFUNGAL EFFECT OF SOME ESSENTIAL OILS AGAINST


FACTORS OF SEEDLING ROOT ROT IN STRAWBERRY

1 1
HAVVA DNLER , SONER EHERL

ABSTRACT
This study was conducted under in vitro conditions to determine antifungal effects of essential
oils from sage (Salvia officinalis), carnation (Dianthus caryophyllus), thyme (Thymus vulgaris L.),
peppermint (Mentha piperita L.), and eucalyptus (Eucaliptus sp. L.) plants against factors (Fusarium
oxysporum, Rhizoctonia solani, Macrophomina phaseolina and Cylindrocarpon destructans) causing
root rot in seedlings of strawberry. 7 day-old cultures of pathogenic isolates which grew in PDA
medium were used. Mycelium discs of growing cultures in 5 mm diameter were inoculated into PDA
medium including different doses (5,10, and 20 l/petri) of plant essential oils and incubated at 24 C
for 7 days. Trials were conducted with 3 repetitions for each of the essential oils. PDA medium which
did not include essential oil were kept as control. After trial, diameters of fungal colonies were
measured and % inhibition rates of essential oils compared to controls were calculated. According to
result obtained, it was found that essential oils from sage, peppermint, carnation, and eucalyptus did
not show a fungistatic effect on mycelial development of Rhizoctonia solani and Macrophomina
phaseolina; and increasing doses of essential oil from thyme had fungicidal and/or fungistatic effect on
Rhizoctonia solani (93% - 100%), Macrophomin aphaseolina (16.5% - 74%),Fusarium oxysporum
(46% - 84%), and Cylindrocarpon destructans(74% - 96%). Essential oils of sage, carnation,
peppermint, and eucalyptus were determined to inhibit mycelial development of Fusarium oxysporum
at the low rate. Essential oil of peppermint had a fungistatic effect on Cylindrocarpon destructans
(20.5% - 25.4%) and sage had a fungicidal effect by inhibiting 100% of mycelia development.

KEYWORDS
essential oil, antifungal activity, strawberry seedling, rot root pathogen

Corresponding Author: HAVVA DNLER, havva.dinler@usak.edu.tr


1
UAK NVERSTES

1612
Poster Session 12
Submission ID: 1602

GOLDENBERRY: USAGE OF FUNCTIONAL FOOD AND ITS


EFFECTS ON HEALTH

1 1
FERIDE AYYILDIZ , MERVE SEYDA KARACIL ERMUMCU

ABSTRACT
The goldenberry (Physalis peruviana) is an exotic fruit that is orginated in South America and
today it is grown commercially in several countries. It is an annual plant that grows all over the world.
It is approximately 2 cm wide and 4-5 g in weight, with smooth, orange yellow skin and it is
containing 100 to 200 small yellowish seeds and protected by the calyx. Although goldenberries are
generally commercialized as fresh products, the fruits are also used in sauces, syrups, and marmalades
or dehydrated similarly to grape raisins for use in bakeries, cocktails, snacks, and cereal breakfast. In
recent years, physical, chemical and nutritional characteristics have been studied goldenberry. The
goldenberries are popular fruits known for their organoleptic properties (flavor, odor and colour) .They
contain high levels of nutritional and antioxidant compounds (provitamin A, vitamin C, bioactive
phenolic compounds, some vitamins of B complex and high levels of fiber). So that general chemical
composition of the goldenberry has many medicinal uses for potential health benefits. Goldenberry is
believed to help maintain a healthy weight (help lose weight) and improve organ function (especially
kidney). Its extracts show antioxidant activity, antiinflammatory activity, antihepatotoxic and
antiproliferative effects on hepatoma cells In addition, this fruit has potential antidiabetic and
antihypertensive effects. Goldenberry have been great interest because of their nutritional and
antioxidant properties. It is a promising candidate functional food but further studies are needed about
its benefits effects on health. References El-Beltagy AE, Naeem MA, Gaafar AM.Optimization and
Quality Attributes of Osmotic Solar Drying of Golden Berry (Physalis peruviana) Journal of
Agroalimentary Processes and Technologies 2013;19(4): 480-489 Franco LA, Matiz GE, Calle J,
Pinzon R, Ospina LF. Anti-inflammatory activity of extracts and fractions obtained from Physalis
peruviana L. calyces. Biomedica. 2007; 27(1):110-5. Pinto Mda S, Ranilla LG, Apostolidis E, Lajolo
FM, Genovese MI, Shetty K. Evaluation of antihyperglycemia and antihypertension potential of native
Peruvian fruits using in vitro models. Journal of Medicinal Foods. 2009;12(2):278-91. Valdenegro M,
Almonacid S, Henrquez C, Lutz M, Fuentes L et al. The Effects of Drying Processes on Organoleptic
Characteristics and the Health Quality of Food Ingredients Obtained from Goldenberry Fruits
(Physalis peruviana). 2013;2: 642. Yldz G, zli N, nal H. Physical and chemical characteristics of
goldenberry fruit (Physalis peruviana L.) J Food Sci Technol 2015;52(3):2320-2327.

KEYWORDS
goldenberry, functional food, nutrition, health

Corresponding Author: FERDE AYYILDIZ, feridecelebi_dyt@hotmail.com


1
GAZ UNVERSTY FACULTY OF HEALTH SCENCES DEPARTMENT OF NUTRTON AND DETETCS

1613
Poster Session 12
Submission ID: 1603

ESSENTIAL OIL COMPOSITION OF LEAF, FLOWER AND STEM OF


STYRAX (STYRAX OFFICINALIS L.) GROWING IN ANTAKYA

1 2
ELIF ATIKKA , ALIME EBRU AYDIN

ABSTRACT
Styrax officinalis L. (Styracaceae) grows around the Mediterranean region and in East and
South-Eastern Asia [1]. S. officinalis was used in folk medicine in the Mediterranean region as
antiseptic and against respiratory diseases [2]. Styrax officinalis L. samples (flower, leaf and stem)
were harvested in May, September and November 2015 in Antakya. First of all, collected materials
were air-dried at the room temperature and then powdered. Later, oil samples were obtained by
hydrodistillation for 4h, by using a Clevenger-type apparatus. Essential oils were obtained in a low
yield of 0.015 %, 0.008 % and 0.005 % for leaf, flower and stem oils, respectively. The composition
of the essential oils of Styrax officinalis L. was determined by GC/MS as below; [1] In the leaf of
samples, (E)-2-hexenal (17.5%), linalool (11.8 %) and geranial (5.6 %) [2] In the flower of samples,
linalool 26.7 (%). [3] In the stem of samples, Tridecanal (9.9 %) and dodecane (9.3 %), while -
terpineol (16.0 %) and eugenol (10.0 %) References: [1] Fritsch, P.W., 1999. Phylogeny of Styrax
based on morphological characters with implications for biogeography and infrageneric classification.
Syst. Bot. 24, 356-378. [2] Kim, Y.S., Shin, D.H., 2004. Volatile components and antibacterial effects
of simultaneous steam distillation and solvent extracts from the leaves of Styrax japonica S. & Z. Food
Sci. Biotechnol. 13, 561-565.

KEYWORDS
Essential oil, Styrax officinalis L.

Corresponding Author: ELF ATIKKA, eliffcatikkas@gmail.com


1
KARAMANOLU MEHMETBEY NVERSTES, ERMENEK MESLEK YKSEKOKULU
2
MUSTAFA KEMAL NVERSTES, FEN EDEBYAT FAKLTES KMYA BLM

1614
Poster Session 12
Submission ID: 1604

DETERMINATION OF ANTIOXIDANT AND ANTIRADIAL


ACTIVITIES OF A NEW IDENTIFIED UNDERGROUND ORCHIDS
(RHIZANTHELLA GARDNERI) PLANT FOR THE FIRST TIME IN
TURKEY

1 1 2
NAZAN DEMR , SEDEF AIK , HAYRUNNISA NADAROLU

ABSTRACT
The plant was collected by Prof.Dr. Nazan DEMR from Mugla Stk Kocman University,
Faculty of Science, Department of Chemistry, and this plant was diagnosed as a different kind of
underground orchid (Rhizanthella gardneri) by the lecturers of Atatrk University, Department of
Biology. This orchid species have undergone, this plant development are underground. Also, it has
opened red-fuchsia flowers and has been found to have a highly effective odor. The underground
orchid species that bloomed in May-June are continuing their work on the registration and
identification as Nazdemir gardneri. In order to evaluate the antioxidant and radical scavenging
activities of this plant, total phenolic compound amount determination, cupric ions (Cu2+) reduction
capacity by the Kuprak method, Fe3+ reduction capacity by FRAP method, superoxide anion radical
scavenging activity (O2-), 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) scavenging activity,
2,2'-azino-bis-(3-ethylbenzthioazoline-6-sulfonic acid) (ABTS+) radical scavenging activity was
-tocopherol were used as standard antioxidants
in the study. It was observed that the Nazdemir gardner plant from the obtained findings exhibited
antioxidative reduction activities in an effective manner. It can be thought that it contributes to the
protection of the soil since it is grown under the soil. Our studies on the bioactivity and protection of
this plant, which is accepted as one of the most valuable flowers in the world, continue.

KEYWORDS
Underground orchid, Nazdemir gardneri, Antioxidant activity, Radical scavenging

Corresponding Author: NAZAN DEMR, nazdemir@mu.edu.tr


1
MULA SITKI KOMAN NVERSTES, KOZMETK RNLER UYGULAMA VE ARATIRMA MERKEZ,
48000 MULA, TURKEY
2
ATATRK NVERSTES, ERZURUM MESLEK YKSEKOKULU, GIDA LEME BLM, 25240, ERZURUM,
TRKYE

1615
Poster Session 12
Submission ID: 1605

TOTAL POLYPHENOL CONTENT OF METHANOLIC EXTRACT


FROM ARUM DIOSCORIDIS SM. VAR. DIOSCORIDIS

1 1 1
ERDAL YABALAK , ZKAN GRMEZ , AHMET MURAT GZR

ABSTRACT
Arum L. is a genus of flowering plants which belongs to Araceae, represented by 26 species
and distributed in Northern Africa, Mediterranean Region, Western Asia, and Europe [1]. Arum
dioscoridis Sm. Var. Dioscoridis (A. dioscoridis) which is traditionally used in the Mediterranean
gastronomy is a member of Arum L. [2]. Phenolic compounds which belong to phytochemical
substances, are secondary metabolite that are generally produced in various plants [3]. In the last few
years, they have gained common attention due to their positive impact on human health. Some of the
benefits of phenolic compounds are antioxidant activity, antimutagenic, anticarcinogenic activities,
anti-inflammatory and neuro-protective effects [4]. In this study, we have investigated total phenolic
content of A. dioscoridis. Total polyphenol content was determined as 221.5 mg GA/100 g dried
weight. Acknowledgements: This study was supported by the Research Fund of Mersin University in
Turkey with Project Number: 2017-1-AP1-2207 References: 1. Farid, M. M., Hussein, S. R., Ibrahim,
L. F., Desouky, M. A. E., Elsayed, A. M, Oqlah, A. A. E., Saker, M. M., Asian Pacific Journal of
Tropical Biomedicine 5 (2015) 944-947. 2. Abu-Reidah, I. M., Ali-Shtayeh, M. S., Jamous, R. M.,
Arraez-Roman, D., Segura-Carretero, A., Food Research International 70 (2015) 74-86. 3.
Limmongkon, A., Janhom, P., Amthong, A., Kawpanuk, M., Nopprang, P., Poohadsuan, J., Somboon,
T., Saijeen, S., Surangkul, D., Srikummool, M., Boonsong, T., Asian Pacific Journal of Tropical
Biomedicine 7(4) (2017) 332-338. 4. Skendi, A., Irakli, M., Chatzopoulou, P., Journal of Applied
Research on Medicinal and Aromatic Plants In press doi: 10.1016/j.jarmap.2017.02.001

KEYWORDS
Arum dioscoridis Sm. Var. Dioscoridis, total polyphenol content, Folin-Ciocalteu

Corresponding Author: ERDAL YABALAK, yabalakerdal@gmail.com


1
MERSN UNVERSTY, FACULTY OF ARTS AND SCENCE, DEPARTMENT OF CHEMSTRY, MERSN TR-33343,
TURKEY

1616
Poster Session 12
Submission ID: 1606

MUCOSAL BURN ASSOCIATED WITH GARLIC IN A PATIENT WITH


DENTAL PAIN: A CASE REPORT

1 2
ZLEM GRMEZ , MELIKE BAARAN

ABSTRACT
Herbal products has been used in many cultures for treating various health problems for
centuries. Garlic (Allium sativum L., Fam Liliaceae) which has widespread pharmacological effects,
has been used as an alternative treatment method by patients in dentistry. But uncontrolled used of it
may lead adverse effects. The aim of this report is to draw attention for the adverse effect occured on
the oral mucosa associated with uncontrolled used of garlic. A 38 year-old male patient was admitted
to our clinic complaining of severe pain on the right mandibular posterior teeth. It was learned from
the anamnesis that the patient had applied garlic topically around his teeth due to the pain. Intraoral
examination revealed a broad mucosal burn which has white plaque appearance with eritematous area
on the right buccal mucosa, gingiva around the right maxillary and mandibular posterior teeth. Also it
was noticed that profund caries at mandibular second and third molar and, maxillary third molar.
Dentists should enquire the patient about the use of herbal products while taking the patient`s history
and be aware of adverse effects can occur from uncontrolled use of herbs like garlic.

KEYWORDS
Garlic, Herbal medicine, Oral mucosa, Mucosal burn

Corresponding Author: ZLEM GRMEZ, ozlemgormez@sdu.edu.tr


1
SLEYMAN DEMREL NVERSTES D HEKML FAKLTES AIZ, D VE ENE RADYOLOJS
ANABLM DALI

1617
Poster Session 12
Submission ID: 1607

ETHNOBOTANICAL USES OF SOME SALVIA L. (LAMIACEAE)


SPECIES FROM HATAY (TURKEY)

1 1 1
FARUK KARAHAN , AHMET LIM , BERNA DEMIR

ABSTRACT
Since ancient times, Salvia L. species have been used in traditional medicine for the treatment
of diabetes and skin diseases. Salvia species are commonly used in Anatolia for colds, stomach aches,
and sore throats. It is the largest genus of the family Lamiaceae, including over 900 species in the
world and represented in Turkey by 94 taxa belonging to 89 species with 50% endemism. This plant is
represented by 15 taxa and locally called Ballk otu, Hint Adaay, Da Reyhan, Misk
adaay and Dadrak in Hatay city. The present study reveals the ethnobotany and traditional
medicinal uses of 12 species of Salvia natural distributed in Hatay province (East Mediterranean of
Turkey). The data on plants included botanical names, vernacular names, the parts used and specific
purpose of use. The common preparation of the medicinal plants in the city are drink as tea and
external. Local people commonly use for the remedy of respiratory system disorders, gastrointestinal
system disorders, skin diseases and antimicrobial. Our results were comparatively discussed with the
other literatures. We believe that fnding of this study will significantly contribute to the
ethnobotanical studies at local or regional scales.

KEYWORDS
Ethnobotany, Traditional medicine, Medicinal plant, Salvia, Hatay.

Corresponding Author: BERNA DEMR, bernademir1067@gmail.com


1
MUSTAFA KEMAL NVERSTES

1618
Poster Session 12
Submission ID: 1608

THE PROFILE OF VOLATIL OILS OF MENTHA SPP. COLLECTED


FROM DIFFERENT LOCATIONS OF TURKEY

1 2 2
HARUN DIRAMAN , AMIR SOLTANBEIGI , AYEGL TRK BAYDIR

D
ABSTRACT
Mentha speices have been consumed for many years in oriental and Mediterrenean culture as
tea and nutritional food addition due to their special avour and aroma properties coming from its

TE
volatile oil. The volatile oil of the various types of the Mentha and the components of these various oil
indicate main variation under effect of the type, variety, ecological conditions, management and
harvesting time. These compositions are mainly used in food, pharmaceutical, cosmetics and
perfumery industries. In this study, the volatile oils obtained by a hydro-distillation method from dried
EN
leaves of mint grown various lacations (Afyon, Kahramanmara and Erzincan) of Turkey are
investigated by gas chromatography-mass spectrometry (GC-MS, Agilent Technologies, 7890B) with
an HP-Innowax column (Agilent 19091N-116: 60 m0.320 mm internal diameter and 0.25 m film
thickness). MS scan range was (m/z): 35-450 atomic mass units (AMU) under electron impact (EI)
ES
ionization (70 eV). Identifications of the mint compounds were confirmed by using the MS database
of US National Institute of Standards and Technology (NIST), Wiley libraries and literature data. The
volatile oil contents of mentha samples were 0.5 1.25 % and refractive indexes were found 1,48148 -
1,49160. The major volatil compounds of dried leave of Mentha samples were identified D-Carvone
(47.83 26.93 %), 18-Crown-6 (12.38- 12.45 %),1.8-Cineole (10.03 12.25 %) , dl-Limonene (3.19 -
PR

10.76) for three locations and in addition, Linalool, (16.09%), Linalyl acetate (6.87 %), 18-Crown-14
(6.83 %) and -Cubebene (4.25%) for Afyonkarhisar sample with representing 99.9%. The high D-
Carvone compound was determined 47,83 % in Kahramanmara sample when the lowest dl-Limonene
3.19 % in Afyonkarahisar sample. There were a remarkable variations among compounds of locations
due to Mentha sub-species, different agro-ecological conditions and various harvest techniques,
storage conditions. This project is supported by Afyon Kocatepe Univesity AK-BAP (Project No:
T

16.MH.05)
O

KEYWORDS
N

Mentha spp, Turkey, volatile oil , refractive index,GC-MS

Corresponding Author: HARUN DIRAMAN, hdiraman@aku.edu.tr


1
AFYON KOCATEPE NVERSTES MHENDSLK FAK. GIDA MHENDSL & GIDA KONTROL
ARATIRMA VE UYGULAMA MERKEZ, AFYONKARAHSAR
2
AFYON KOCATEPE NVERSTES GIDA KONTROL ARATIRMA VE UYGULAMA MERKEZ,
AFYONKARAHSAR

1619
Poster Session 12
Submission ID: 1609

DETERMINATION OF CHARACTERISTICS OF INSTANT TEA


PRODUCED FROM THYME (THYMUS VULGARIS)

1 2 1 1 1
CEMAL KAYA , ZEYNEP AKIT , SEMRA TOPUZ , HSEYIN AKIT , MUSTAFA BAYRAM

D
ABSTRACT
Herbal teas are prepared by brewing of dried plant leaves, flowers, fruits or roots in warm or

TE
hot water. Thyme (Thymus vulgaris) is widely consumed as spice in Turkey. Thyme which has
essential oil, is used as natural antioxidant. In this study, it was aimed to determine some important
properties of water soluble herbal tea powders produced by spray drying and freeze drying techniques
from extracts obtained by applying the classic brewing and boiling method of thyme plant.Instant tea
powders were obtained by spray drying and freeze drying techniques from watery extract of thyme.
EN
Some physical and chemical properties of these tea powders were investigated. Solubility in water,
moisture content, yield, Hunter colour values (L*, a*, b*), total phenolic compounds and total sugar
quantities were determined. The antioxidant activity of instant tea powders were evaluated with FRAP
and TEAC tests. Some individual phenolic compounds and flavor compounds of thyme tea powders
ES
were determined by HPLC and GC-MS, respectively. Moreover, instant thyme tea powders were
evaluated for their sensory properties. The results were compared with traditional brewing
method.Solubility in water of thyme tea powders by using freeze drying and spray drying techniques
were found 96,00; 98,67 and moisture content were found 7,31; 0,18%. Yield of tea powder produced
from thyme plant (traditional; freeze dried; spray dried) were found 14,66; 22,10; 21,27 %. Moreover,
PR

the hunter colour values of thyme teas prepared by using traditional method, freeze dried powder,
spray dried powder were 27,43; 26,12; 25,27 L*, 4,01; 4,70; 5,28 a*, -3,25; -2,44; -2,18 b*. Total
phenolic content of tea produced from thyme (traditional; freeze dried; spray dried) were found as
18,90; 30,85; 34,60 mgGAE/100 mL and total sugar quantities were found as 33,93; 37,27; 47,06
mgGE/100mL. FRAP activities were found as 1301,27; 1573,59; 1794,51molTE/100 mL and TEAC
activities were found as 3807,15; 3949,70; 3967,52 molTE/100 mL, respectively. Spray dried herbal
T

tea powders had less flavoring agents because of heat application during drying. Thyme contains
volatile oil, carvacrol. The boiling point of carvacrol is 237.7 C. Due to the high boiling point, the
O

presence of carvacrol residue in the powdered thyme tea is an expected result. But; other thyme
volatile components with lower boiling point are lost during the thermal process applied in production.
N

But, other thyme volatile components with lower boiling point were lost during the thermal process in
the production process. When the results of the sensory analysis were examined, the highest average
was obtained by traditional method and followed by freeze drying techniques, spray drying,
respectively. In this sense, the most important factor may be preservation or losing of volatile aroma
components during processes. Because of the unique aroma of the most important feature of thyme
tea, it is suggested to add flavor to the thyme tea produced by the spray drying technique.

Corresponding Author: CEMAL KAYA, cemal.kaya@gop.edu.tr


1
GAZOSMANPAA NVERSTES
2
GMHANE NVERSTES

1620
KEYWORDS
Thyme (Thymus vulgaris), instant tea , spray drying, freeze drying

1621
Poster Session 12
Submission ID: 1610

DETERMINATION OF STANBUL BELGRADE FOREST


PHARMACOLOGICAL OPTION VALUE BY PEARCE-
PUROSHOTHAMAN (P & P) MODEL

1 2 2 3
MEHMET ALAN , ALI GREL , MEHMET ZDEMIR , AHMET TUTAR

ABSTRACT
The Belgrad Forest is located on the European side of Istanbul, north of the residential areas,
between 28 53''25''29 00''55 '' east latitude and 41 09'' 44'-41 14''40'th latitude . The forest
begins to descend in the southeast direction of the Yldz Mountains as a land structure, resulting in a
slight fluctuation in the Istanbul Strait. The highest point is Kartaltepe in the northern part with a rise
of 230 meters. Another notable elevation is Kokmutepe in the north-east of the forest with an
elevation of 219 meters. The lowest point within the forest (Kurudere) is 40 meters above sea level.
The Belgrade Forest, a natural and cultural heritage, has a flora and variety of plant taxon varieties. It
can be seen that the plant contains not only the Balkan Flora in terms of geography but also colchic,
mediterranean and central european flora elements which are also in character. In this direction, there
are approximately 400 plant taxa and 6 endemic plant taxa in the Belgrad Forest, which has an area of
only 5524 hectares. Because of Istanbul's special position, the Belgrad Forest, which has a wide
variety of climate, soil and ground forms, allows this variety to live in a wide variety of different
species of plants and animals within the forest. The Belgrad Forest, which is the closest forest area to
the city center in Istanbul, has been subject to numerous scientific researches due to its importance for
the past and present day. One of the most researched forests in Turkey has come into existence. Until
now, however, no study has been conducted on the pharmacological option value of this forest, which
is the source of this rich biodiversity. The Pearce and Puroshothaman model is based on the rental
income approach of Ruitenbeek (1989). This approach is based on the assumption that countries with
herbal genetic material that will be necessary for the pharmaceutical sector in the future will need to
obtain these assets - rent income, or annuity, in exchange for using the firm, and that this lease value
will reflect the pharmacological value of plants. Pearce and Puroshothaman (1992), based on the rental
income approach, expressed the medical (pharmacological) value of a plant in a protected biodiversity
field that could produce the drug "D" in the future. In this study, pharmacological substances and
drugs that can be obtained from Belgrade forests, pharmacological option value according to Pearce
and Puroshothaman model; The low estimate was set at 70.60 per unit area, 905.14 with the medium
estimate, and $ 12671.97 / ha / year with the high estimate.

KEYWORDS
Belgrad Forest, Pharmacological Option Value, P & P Model

Corresponding Author: MEHMET ALAN, mehmetalan@ogm.gov.tr


1
ORMAN GENEL MDRL
2
ORMAN GENEL MDRL, MARMARA ORMANCILIK ARATIRMA ENSTTS
3
SAKARYA NVERSTES, KMYA BLM

1622
Poster Session 12
Submission ID: 1611

THE CHERRY LAUREL: COMPOSITION AND MPACT ON HUMAN


HEALTH

1 1
ENOL KSE , YAMUR ERM KSE

D
ABSTRACT
Cherry laurel (Laurocerasus officinalis) is a typical summer fruit from the eastern Black Sea
Region of Turkey and locally called Karayemi or Taflan. It is consumed as fresh, dried, marmalade,

TE
jam, canned and pickled forms in Black Sea Region. Cherry laurel is used in cosmetics industry and
treatment of some diseases for example stomach ulcers, bronchitis, eczemas, hemorrhoids and etc.
Also t is used food additives as flavoring agent. The annual consumption of this fruit is not known
precisely because it is sold in local markets in Black Sea Region. It is getting popularity at a
EN
commercial scale in the United States, Europe, Asia and Turkey. These fruits contain high levels of
micronutrients and phytochemical constituents. Although individual phytochemical constituents of
cherry laurel have been studied for their biological activities, the effects of these compounds on human
health are not entirely clear. The aim of this review is to outline some physical, mechanical, chemical
ES
properties, and bioactive compounds and impact on human health of cherry laurel.

KEYWORDS
PR

Cherry laurel, bioactive compounds, human health


T
O
N

Corresponding Author: ENOL KSE, senolkose28@gmail.com


1
YUZUNCU YIL UNIVERSITY, ENGINEERING FACULTY, FOOD ENGINEERING DEPARTMENT

1623
Poster Session 12
Submission ID: 1612

MINERAL COMPOSITION OF SOME MEDICINAL MUSHROOM


SPECIES GROWN IN VAN PROVINCE (TURKEY)

1 1 1
SEMA SEZGIN , ABDULLAH DALAR , YUSUF UZUN

ABSTRACT
Minerals are vital chemical compounds for humans which have crucial functions such as
maintaining acid-base balance, the osmotic regulation of fluid and oxygen transport in the body and
also playing important roles in the catalytic processes within the enzyme system associated with the
metabolic, endocrine and immune systems (Koyyalamudi et al., 2013). Mushrooms have been used as
important dietary supplements because of their pleasent tastes, nutritional and pharmaceutical
properties such as rich protein, low-fat content, secondary metabolites, vitamins and minerals.
Tricholoma scalpturatum (Fr.) Qul. (Tricholomataceae), Neolentinus cyathiformis (Schaeff.) Della
Maggiora & Trassinelli (Polyporaceae), Chlorophyllum agaricoides (Czern.) Vellinga (Agaricaceae),
Tricholoma populinum J.E. Lange (Tricholomataceae) and Lycoperdon utriforme Bull. (Agaricaceae)
are five edible-medicinal mushrooms species consumed for their nutritional and therapeutic properties
by local people of Van province. The aim of the present study was to evaluate the mineral composition
of five edible-medicinal mushrooms naturally grown in Van province, Turkey. The mushroom
samples (fruiting bodies) were collected from wild areas on October 2016. Different extraction
methods were applied in order to reveal the mineral composition of the samples comprehensively. The
extractions were analyzed by Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES)
and Atomic Absorption Spectroscopy (A.A.S). Within this presentation the concentration of Ag, As,
B, Ba, Be, Cd, Co, Cr, Cu, K, Mg, Mn, Mo, Ni, Pb, Sb, Se, Si, Ti, V, Zn, Na, Ca and Fe of the extracts
prepared from five edible-medicinal mushroom species will be presented in light of scientifically
proven physiological activities if available will be presented. References: 1. Koyyalamudi, S.R. Jeong,
S.C., Manavalan, S., Vysetti, B., Pang, G. (2013). Micronutrient mineral content of the fruiting bodies
of Australian cultivated Agaricus bisporus white button mushrooms. Journal of Food Composition and
Analysis, 31:109-114.

KEYWORDS
Mineral, ICP-OES, AAS, Extraction

Corresponding Author: SEMA SEZGN, semas.ecz@gmail.com


1
YZNC YIL NVERSTES, ECZACILIK FAKLTES

1624
Poster Session 12
Submission ID: 1613

DETERMINATION OF ANTIOXIDANT PROPERTIES AND


VOLATILES IN RAW AND PICKLED GREEN/BLACK OLIVES

1 2
ZEHRA KASIMOLU , HILAL AHIN-NADEEM

ABSTRACT
Olive (Olea europea), a member of Oleaceae, is native tree of the Mediterranean climate. Its
pickled fruits are consumed in daily diet, especially in the breakfast. Olive oil obtained from fruits is
also frequently used in the world. The olive may be green or black according to the degree of ripening
at harvest. Ripening of olive is a long process that takes place in 6-8 months. The optimum harvesting
time differs according to the purpose of the olive processing. If the green table olives are to be
processed, the fruits should be harvested when their color turns to yellowish-green (September-
October). On the other hand, if black olive is desired, the fruits are harvested after the fleshy part
become darker (November-December). To get oil, the olives are to be harvested when there is no
green fruit in the tree. In this study, dry matter (gravimetric method), total phenolic content
(spectrophotometric by using Folin Ciocalteu assay), antioxidant activity (spectrophotometric by using
DPPH radical scavenging assay) and volatile components (by SPME-GC-MS) were determined for the
Memecik type green and black olives. The samples were obtained from the local processor of Milas
(Mula province, Turkey) during the pickling period of 0, 12, and 25 days. At the end of pickling, dry
matter content of the green and black olives increased from 22.89 to 44.71% and from 41.78 to
69.66%, respectively. Total phenolic content and antioxidant activity decreased during the pickling.
The total phenolic content of the raw green and black olives, which were initially 230.22 and 202.51
mg/100g, respectively, decreased to 190.12 for green olives and to 192.21 mg/100g for black olives
after 25 days of pickling. Similarly, the EC50 values of 0.36 and 0.44 g/mg DPPH of the raw green
and black olives, respectively, increased to 0.40 in the green olive and 0.52 g/mg in the black olive
after 25 days, so that the antioxidant activity decreased. GC chromatogram showed 13 volatiles in the
raw green olive while 12 were found in the raw black olive (peak areas greater than 1 %). The main
volatile compounds extracted from raw green olive by SPME method (using PDMS / DVB fiber) were
3-O-methyl-D-fructose (19.50%), hexanal (16.52%), butanol (13.96%), butan-2-one (11.76%) and 2-
propanamine (8.65%) while butanol (18.11%), n-pentanal (14.23%), carbamic acid (9.52%), hexanal
(9.32%) and formic acid (8.35%) were the main volatiles in the raw black olive. At the end of
pickling, the major volatile components in the green olive were acetic acid (31.79%), mannopyranose
(10.76%), D-fructose (10.23%), pentanal (7.25%) and 1,3-propanediol (7.05%) whereas ethanol
(31.99%), butanol (18.25%), acetic acid (8.84%), D-mannofuranoside (8.50%) and 1,3-propanediol
(4.56%) were in the pickled black olive.

KEYWORDS

Corresponding Author: HLAL AHN-NADEEM, hilal.nadeem@adu.edu.tr


1
AKDENZ UNVERSTY, DEPARTMENT OF FOOD ENGNEERNG
2
ADNAN MENDERES UNVERSTY, DEPARTMENT OF FOOD ENGNEERNG

1625
Olive, pickling, total phenolics, antioxidant activity, volatiles

1626
Poster Session 12
Submission ID: 1615

EFFECTS OF DRYING TECHNIQUES ON SOME QUALITY


PROPERTIES AND LIMONENE CONTENT OF ORANGE PEEL

1 2 3
HAMZA BOZKIR , YELIZ TEKGL , TANER BAYSAL

D
ABSTRACT
Excessive amounts of solid waste occur in fruit processing plants and consumption of fruit.

TE
When these wastes are released directly to the environment, they can cause environmental pollution,
besides, wastes that can be used for the production of valuable biomass and nutrients will be lost. For
this reason, utilization of food industry wastes provides added value in economic terms. Moreover, it
also provides benefits in terms of health and nutrition because the mentioned wastes contain valuable
components for human metabolism and enrichment of foods. As a result of orange peel contains
EN
ingredients that are important for nutrition, the free radicals that build are strengthening immune
system. The orange peel with high bioavailability can be dried and used in the food, pharmaceutical
and cosmetic industries. The purpose of this study is to investigate the effects of drying techniques on
some quality characteristics (color, vitamin content, total phenolic content, anthocyanin content, bulk
ES
density, rehydration capacity) and limonene content of the orange peel. Vacuum microwave dryer (877
mbar, 50 C, 334 watts) and tray dryer (50 C, 1.5 m/s) were used. It has been found that the vacuum
microwave dryer reduces the drying time compared to the tray dryer. Limonene content of orange peel
increased with drying process. It has been found that the vacuum microwave dryer significantly
preserves color (L*, a*, b*, E, Hue, and C), vitamin C content, aroma components and total
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phenolic content more than tray dryer.

KEYWORDS
orange peel, color, drying, quality, limonene
T
O
N

Corresponding Author: YELZ TEKGL, yeliztkgl_gida@hotmail.com


1
MUNZUR UNVERSTY, FACULTY OF ENGNEERNG, DEPARTMENT OF FOOD ENGNEERNG, TUNCEL
2
ADNAN MENDERES UNVERSTY, KOSK VOCATONAL SCHOOL, FOOD PROCESSNG DEPARTMENT, AYDIN
3
EGE UNVERSTY, FACULTY OF ENGNEERNG, DEPARTMENT OF FOOD ENGNEERNG, IZMR

1627
Poster Session 12
Submission ID: 1616

KONJAC (AMORPHOPHALLUS KONJAC) FLOUR

1
YAMUR ERM KSE

ABSTRACT

D
Although Konjac (Amorphophallus konjac) is an unknown plant in Turkey, it has been used in
countries like China, Japan, Indonesia and South East Asia for many years as a food source and as a
traditional medicine. It is a large, perennial exotic plant with large starchy corms (underground storage

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organ) and a single large leaf. Konjac flour is obtained from the corm which is washed, peeled, sliced,
dried and ground. The flour is known to be a valuable source for glucomannan; a high-molecular
weight and water-soluble polysaccharide (dietary fibre). The flour is used as a functional food in the
form of noodle, spaghetti, rice, tofu and snacks. These foods lower blood sugar in patients with diabets
EN
due to konjac glucomannan delays glucose absorption. Furthermore these foods may help weight loss
by filling the stomach and promote colonic mucosal health due to increase fecal weight and reduce
transit time by dietary fiber. It is also widely used in food and pharmaceutical industries as a
emulsifier and stabiliser because of its valuable properties for example it is easily dispersed in water, it
is a favorable thickening agent and it has high water absorption. Konjac gel is a well-known traditional
ES
medicine in China for more than 2000 years. It is prepared from konjac flour and it is used for the
treatment of asthma, breast pain, infection, burns, atopic skin disorders etc. The purpose of this paper
was to introduce the konjac flour in a larger scale. Thus, use of the konjac plant in both food and
pharmaceutical industry may increase.
PR

KEYWORDS
Amorphophallus konjac, Konjac glucomannan, Konjac flour, Traditional medicine,
Functional food,
T
O
N

Corresponding Author: YAMUR ERM KSE, yagmurerim@yyu.edu.tr


1
YUZUNCU YIL UNVERSTY, FACULTY OF ENGNEERNG, DEPARTMENT OF FOOD ENGNEERNG

1628
Poster Session 12
Submission ID: 1617

THE USE OF MEDICAL AND AROMATIC PLANTS AND SOCIO


ECONOMIC LIFE

1 1 1 1
AYHAN SAYI , HLYA SAYI , MEHTAP BAYKAL , CELAL ERBA

ABSTRACT
Our country, which has a rich biodiversity, is home to countless medical and aromatic plants.
Since the early days of human history, plants have been characterized as a fundamental resource for
the production of medicines used to protect human health, as well as providing the nutrients that
humans need for the continuation of human life. Therapeutic use of plants has begun with the history
of mankind, and people have used the therapeutic power of plants to solve their health problems and
stay healthy. Herbal medicines, which are common in local medical practices, also called alternative
medicine, have been produced at the end of long experiences and are widely used today. Many
medicines used in modern art are also derived from plants. It was unthinkable that medicinal and
aromatic plants with so much precaution did not fall within the scope of economic activities that
produced solutions for human needs. As a result, medical and aromatic plants are estimated to reach $
95 billion in 2015, while the market is expected to reach $ 110 billion by the end of 2016. Turkey's
medical and aromatic plants are worth $ 2.5 billion (Source: Dnya Gazetesi 16.03.2017). These
figures are proof that our country's medical and aromatic plant diversity is a new opportunity for our
people to survive in the countryside. The employment provided in the production process for the
medical and aromatic plants of our country will improve the socio-economic situation of the people
living in the rural areas by providing justice in the income due to the high income to be provided while
being a solution to the problem of unemployment. In this study, it is aimed to investigate the
advantages provided by the production of medicinal and aromatic plants and socio-economic
development effects.

KEYWORDS
Medical Plants, Aromatic Plants, Human Health, Traditional Usage of Plants, Economy

Corresponding Author: AYHAN SAYI, asaygi@cu.edu.tr


1
UKUROVA NVERSTES YUMURTALIK MESLEK YKSEKOKULU

1629
Poster Session 12
Submission ID: 1618

INVENTORY OF MEDICAL AND AROMATICAL PLANTS

1
DERYA MUMCU KKER

ABSTRACT

D
Interest in medicinal and aromatic plants has greatly increased recently due to their
commercial, nutritional and medical importance. These plants derived from natural forests are used as
pharmaceuticals, herbal remedies, flavourings, perfumes and cosmetics, and other organic products.

TE
Utilization from medicinal plants as traditional remedies is very common for rural population in
developing countries. World Health Organization (WHO) decelerated that about 80% of world
population still is used medical plants for remedies. Medicinal and aromatic plants play a critical role
for health service and rural development because of their additional income. Turkey is considered to
EN
be a rich country in terms of plant diversity. Of the estimated 11,707 plant species as well as sub
species and varieties (Gner et al. 2012) naturally occurring in the country, about 1/3 are considered as
aromatical plants. While about 347 medical and aromatical plants with their parts of leaves, roots,
bark, flowers or fruits are consumed in the domestic markets, about 100 plants are exported to the
international markets (Baer, 1997). The high economic value in national and international markets
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caused to increase harvesting of these plants by rural people. Remarkable amount of medical and
aromatic plants belonging to about 100 species are being harvested. The urgent need for sustainable
utilization from medicinal and aromatic plants is characterizing their species diversity, spatial
distribution, abundance and ecology with suitable inventory methods. Because these studies are
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difficult to measure or time and money consuming for all species, these information should be
prepared for economically priority plants. Beside inventory methods, inventory time, sample size and
shape for each medicinal and aromatic plants and their beneficial parts have serious deficiencies. This
paper tries to highlight all the important aspects in inventory of medicinal and aromatic plants and
identify some useful inventory methods for these products.
T

KEYWORDS
O

Plant-based NWFP, Sustainable Forest Management, Inventory


N

Corresponding Author: DERYA MUMCU KKER, dmumcu@ktu.edu.tr


1
MS

1630
Poster Session 12
Submission ID: 1619

STEM AND LEAF ANATOMY OF FOUR MEDICINAL


REPRESENTATIVES OF CREPIS L. (ASTERACEAE)

1 1 1 1
KEMAL VEHBI MAMOLU , HSEYIN NCEER , ZGE DUMAN , SEMA HAYIRLUOLU-AYAZ , NURSEN AKSU-
1
KALMUK

ABSTRACT
The genus Crepis L. belongs to the tribe Cichorieae of the Asteraceae family. Most of this
genuss members have Crepin matter. Crepis foetida subsp. foetida, C. foetida subsp. rhoeadifolia,
C. vesicaria and C. zacintha are medicinal plants which are used in the folk medicine. However, no
stem and leaf anatomy of these medicinal plants have been studied till now. Anatomical studies were
performed in the stem and leaf. Transverse sections from middle parts of the stem were taken by hand
using commercial razor blades. The leaf anatomy has been observed both side of the leaves. The
anatomical characters were measured using an ocular micrometer under the light microscope. The
stem structure of Crepis foetida subsp. foetida, C. foetida subsp. rhoeadifolia, C. vesicaria and C.
zacintha is composed of epidermis, collenchyma, parenchymatous cortex and pith. These species have
anomocytic stomata in both the upper and the lower surface of the leaves. This study was supported by
the Scientific and Technological Research Council of Turkey (TUBITAK, Project no. 112T132).

KEYWORDS
Crepis, anatomy, stem, leaf

Corresponding Author: KEMAL VEHB MAMOLU, kvimamoglu@ktu.edu.tr


1
KARADENZ TECHNCAL UNVERSTY, FACULTY OF SCENCES, DEPARTMENT OF BOLOGY, 61080
TRABZON

1631
Poster Session 13
Submission ID: 1622

THE INVESTIGATION OF BIOLOGICAL ACTIVITIES AND HPLC-


DAD-MS ANALYSIS OF MAJOR PHENOLIC COMPOUNDS OF
HELICHRYSUM PLICATUM DC. SUBSP. PLICATUM

1 1 2 3 1
TUBA GEZMI , TURGUT TAKIN , DUYGU TAKIN , BERNA ZBEK ELIK , SMAIL ENKARDE , SEVDA
1
SZGE SELUK

ABSTRACT
The tribe Gnaphalieae of Asteraceae comprises 185 genera and more than 1240 species.
Helichrysum Mill. is the largest genus of this tribe, including approximately 600 species occurring in
Europe, Asia, Africa and Madagascar. This genus which is represented by 24 species, 30 taxa of
which, 17 are endemic, has been recorded in the Flora of Turkey (1). Helichrysum species are largely
known as lmez iek, altnotu or mantuvar in Anatolian folk medicine. Helichrysum species have
been used as folk medicine for at least 2000 years against gall bladder disorders in the form of
medicinal teas, because of their bile regulatory and diuretic effects. In Turkey several Helichrysum
spp. are used in folk medicine for removing the kidney stones and as diuretics. The diuretic and bile
regulatory effects of the Helichrysum spp. are due to the flavonoids that they contain (2). In Turkish
folk medicine, H. plicatum DC. subsp. plicatum has been used as a diuretic, antidiabetic, lithagogue
and for stomach ache (3). The aim of this study was to investigate phenolic composition and compare
evaluation of biological activities of H. plicatum subsp. plicatum. In the present study, The total
flavonoids contents, antioxidant, anti-urease, antimicrobial activities of different extracts from H.
plicatum subsp. plicatum were compared. The extracts were quantitatively analyzed for total flavonoid
contents using aluminium chloride colorimetric assays. Antioxidant activity of the extracts was
measured using the DPPH., ABTS.+ scavenging activities and FRAP assay. The urease inhibitory
activities of the extracts were determined according to a reported method (4). In addition,
antimicrobial activities of extracts were investigated using microdilution methods (5). The methanol
extract obtained by using Soxhlet method of H. plicatum subsp. plicatum had the highest amounts of
total flavonoids and showed the highest DPPH. radical scavening and ferric reducing activity.
Subsequently, we tried to identify the major phenolic compounds in methanol extract by HPLC-
DAD/ESI-Q-TOF LC/MS. The major phenolic compounds were identified as: chlorogenic acid,
dicaffeoylquinic acid, luteolin, luteolin 7-O-glycoside, naringenin-O- hexoside and isoquercetin.
References: 1. ztrk B, zek G, zek T, Baer KHC, Chemical Diversity in Volatiles of
Helichrysum plicatum DC. subspecies in Turkey, Rec. Nat. Prod. 2014; 8(4) 373-384. 2. Suzge S,
Merili AH, Houghton PJ, ubuku B, Flavonoids of Helichrysum compactum and their antioxidant
and antibacterial activity, Fitoterapia, 2005; 76 )269-272. 3. Kolayl S, ahin H, Ulusoy E, Tarhan ,
Phenolic Composition and Antioxidant Capacities of Helichrysum plicatum, Hacettepe J. Biol. &
Chem., 2010; 38 (4) 269-276. 4. Ghous T, Akhtar K, Nasim FUH, Choudhry MA. Screening of

Corresponding Author: TURGUT TAKIN, turguttaskin@marmara.edu.tr


1
MARMARA NVERSTES
2
STANBUL YEN YZYIL NVERSTES
3
STANBUL NVERSTES

1632
selected medicinal plants for urease inhibitory activity, Biology and Medicine, 2010; 2 (4): 64-69. 5.
National Committee for Clinical Laboratory Standards. Reference, Wayne, PA. (2002) 22(15).

KEYWORDS
H. plicatum subsp. plicatum, phenolic compounds, biological activity, Q-TOF LC/MS

1633
Poster Session 13
Submission ID: 1623

SUSTAINABLE PLANNING OF MEDICAL AND AROMATICAL


PLANTS

1
DERYA MUMCU KKER

D
ABSTRACT
Turkey forest ecosystems have abundant resources of medicinal and aromatical plants. In case

TE
the forest ecosystems are managed based on sustainable production, these natural resources provides
economic, ecologic and social-cultural benefits to the people. Medicinal and aromatical plants and
mushrooms with their various parts of fruit, leaf, root, flower and bark are vital forest resources and
have to be integrated into multiple-use forest management plans. However, these economically
important plants is being threatened with forest destruction, land use changes and excessive collection
EN
from their natural habitats. Therefore, there is an urgent need to integrate these products into multiple-
use forest management plans both conservation and utilization. The initial step towards the integrated
management is to perform the inventory and documentation of existing medicinal and aromatical
plants. All information on quality and quantity of population as well as their location. It would be
ES
necessary to identify priority medicinal and aromatical plants and to decide priority interventions and
policies on conservation and harvesting. The second one is to estimate the relationship between these
valuable products and climatic, topographic and stand parameters and to develop empirical models to
predict the annual yield of these products. The last step of medicinal and aromatical plants integrated
management is to use decision support systems enabling decision making process with trade of
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analysis of both timber and related non-wood forest products (NWFP). Though much information
exists on the species diversity in medicinal and aromatical plants in the country, relatively very little is
known about the spatial distribution, productivity and ecology of the great majority of medicinal and
aromatic plants. This study explains the fundamental components of joint production of timber and
medicinal and aromatical plants and conceptional framework of the integration of medicinal and
aromatical plants into multiple-use forest management plans. In addition, this paper presents some
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examples of integration of medicinal and aromatical plants into forest management plans.
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KEYWORDS
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Forest management, Non-Wood Forest Products, Multiple use planning

Corresponding Author: DERYA MUMCU KKER, dmumcu@ktu.edu.tr


1
KARADENZ TECHNCAL UNVERSTY

1634
Poster Session 13
Submission ID: 1624

APOPTOTIC EFFECT OF FERULIC ACID ON MCF-7 HUMAN


BREAST CANCER CELL LINE

1 1 1 1 2 2
CANAN EROLU , EBRU AVCI , HASIBE VURAL , ERCAN KURAR , MCAHIT SEME , YAVUZ DODURGA

ABSTRACT
Breast cancer is the second considering the most common cancer-related mortality in women.
In the worldwide, about 1 million people are diagnosed with breast cancer each year. In recent years,
studies have been focused on investigating anticancer features of various phytochemical compounds.
These compounds can be used as complementary or alternative therapeutic agents. Ferulic acid (FA; 4-
Hydroxy-3-methoxycinnamic acid) is known as an abundant phenolic compound found in various
fruits and vegetables. It has been described to act as a potent antioxidant and the anticarcinogenic
effect of FA has been demonstrated through studies involving various cancer cell lines. The aim of
study was to investigate the effects of FA on apoptosis in MCF-7 human breast cancer cell line. The
effect of FA on cell viability was determined by using XTT method. Total RNA was isolated with
TRIzol Reagent. Expressions of genes are important in apoptosis including BAX, BCL2, CASP3,
CASP7, CASP8, CASP9, CYCS and FAS were evaluated in control and dose groups by qPCR. IC50
dose of FA was found as 600 M for 72h in MCF-7 cells. When compared with the control group,
qPCR results illustrated that a significant increase was observed in the expressions of FAS, CASP3,
CYCS and CASP9 genes as 1.85, 2.35, 2.67 and 5.82 folds respectively, whereas there was a decrease
in the expression of BCL2 gene as 1.91 fold. In conclusion, it is thought that FA demonstrates
apoptotic effect by regulating expression of important genes in apoptosis on MCF-7 cells. However,
further molecular and functional analyses are required to clarify its effect on breast cancer.

KEYWORDS
Apoptosis, Ferulic acid, MCF-7 cells

Corresponding Author: EBRU AVCI, ebruavc.87@gmail.com


1
DEPARTMENT OF MEDCAL BOLOGY, MERAM FACULTY OF MEDCNE, NECMETTN ERBAKAN
UNVERSTY, KONYA
2
DEPARTMENT OF MEDCAL BOLOGY, FACULTY OF MEDCNE, PAMUKKALE UNVERSTY, DENZL

1635
Poster Session 13
Submission ID: 1625

DETERMINATION OF SOME BIOACTIVITIES AND CHMEMICAL


COMPOSITION OF TULIP (TULIPA ARMENA) PLANT &
INVESTIGATION OF USABILITY AS HOMEOPATIC DRUGS

1 1 1 2
NAZAN DEMR , ZGE AKIN , YAAR DEMR , FATIH UCKAYA

ABSTRACT
Tulip (Tulipa armena) plant is from family of Liliaceae. It is a perennial herbaceous plant. The
tulip has a special place in eastern culture and mythology. Also it has a unique place in our culture. It
has name after a historical period. More than 100 tulip cultivars naturally grow in our soil and a large
part of them is endemic. The tulip, a kind of national flower, therefore it has specially selected in our
study and it has been investigated whether it can be used in the production of homeopathic medicines
by getting some information from the public. Homeopathy is based on the principle of "heal similar
alike". A disease is treated only with the substance that produces an indication similar to the patient's
complaints. Homeopathy was found by German physician Samuel Hahnemann in the early 18th
century and it is an alternative treatment system that helps the body develop itself naturally. According
to the data of the World Health Organization, it is the most commonly used complementary medicine
method. In Europe, more than 50% of people are treated with homeopathic treatment. Furthermore
more than 50% of doctors recommend homeopathy together with other treatment modalities.
Homeopathic medicines are produced by the orginal material being stored in water or alcohol followed
by a series of dilution and mixing methods. For this purpose, the tulip were collected from Mugla
countryside, defined as botanical followed by content analysis was performed with LC MS. Moreover,
this research include incestigation of protein, phenolic component and some enzyme activities
(protease and peroxidase). Three-phase partitioning method was used for purification of enzyme from
flowers of Turkish tulip. The values of optimal pH and optimal temperature were determined. The
SDS-PAGE technique was used to check the purity of the purified enzyme and determine the number
of subunits, if any. The molecular weight was also calculated using gel filtration chromatography. As a
result, the protease enzyme was purified from Turkish tulip flowers and its phenolic components were
determined. It has been found that the purified protease enzyme has high activity. The plant extracts
were prepared in different organic solvents and water. Forthermore Dilution grades of extracts were
determined for animal experiments. As a result of preliminary surveys and content analyzes, it is
understood that the plant is a flower that can be used in homeopathy. It is planned that it is submitted
to the approval of The Ministry of Health as a traditional and complementary product by optimizing
usability as a medicine. REFERENCES [1] Lehninger, A. L. (2013) Principles of biochemistry, Worth
Publishers Inc., New York, 1152s. [2] Demir N., Demir Y., Kaya E. and Aydn B., Cysteine Protease
from Primrose (Primula vulgaris), Asian Journal of Chemistry, 24(4), 1479-1482 2012. [3] Demir
N.,Ukaya F.,2015 Citrus sinensis (L.) Osbeck (Orange) and Citrus lemon (L.) Burm. F. Purification

Corresponding Author: NAZAN DEMR, demirn@yahoo.com


1
MULA SITKI KOMAN NVERSTES, KOZMETK RNLER UYGULAMA VE ARATIRMA MERKEZ,
MULA
2
BEZMALEM VAKIF NVERSTES, ECZACILIK FAKLTES, STANBUL

1636
of Protease and Peroxidase Enzymes from Lemon Flowers and Investigation of Endustrial Usability,
44-46s.

KEYWORDS
Tulip (Tulipa armena), Homeopathic medicine, Protease enzyme activity, Phenolic
compounds

1637
Poster Session 13
Submission ID: 1627

NATURAL ANTIMICROBIAL SUBSTANCES OF VEGETABLE


ORIGIN

1 1
TUBA BYKSIRIT , HAKAN KULEAAN

ABSTRACT
The main goal of food technology is to prevent food spoilage, thereby ensuring food safety in
foods is to take control of microorganisms. Therefore, adding additives to the product of microbial
activity declines biggest advantage, which can maintain stability for a long time after opening the
package of the food. Food industry, competition for the market share to be effective and must meet the
demands of consumers. Consumers too much untreated, naturally, at least at the level of protective
foods containing chemicals are wanted. Investigate the possibility that new types of antimicrobial drug
use is encouraged. Artificial preservatives due to their adverse effects on the health of consumers has
increased the interest for natural antimicrobial substances. In recent years, for medicinal purposes and
the use of plant nutrients kept focused on the research and therefore the importance of using plants as
natural antimicrobials is increasing every day. Natural protection systems of animal, plant or microbial
origin are used in the protection of foods. Antimicrobial agents obtained from natural sources such as
plants has managed food safety and plant extracts naturally in foods can be used as an antimicrobial
has been proven by scientific research. More than 1340 types of plants formed about 80 of them from
spices and herbs, most of them have a small molecular weight is known to be a source of potential
antimicrobial agents. Studies made until today proved antimicrobial effect of plant extracts such as
Parmelia furfuracea L. Zopf. (lichen), Crocus chrysanthus Herbert (crocus), Myrtus communis L.
subsp. communis (mersin), Asphodelus aestivus L. (iris) Eugenia caryophyllata Thunb. (carnation),
Artemisia absinthium L. (wormwood herb), Ornithogalum Umbellatum L. (akyldz), Hedera helix
(ivy), Datura stramonium (pipeflower), Ficus carica (fig), Avena sativa (oat), Xanthium strumarium
(ptrak grass), Nicotiana tabacum (tobacco) etc.

KEYWORDS
Antimicrobial, artificial preservatives, natural preservatives, food safety

Corresponding Author: TUBA BYKSIRIT, tubabuyuksirit@sdu.edu.tr


1
SLEYMAN DEMREL NVERSTES

1638
Poster Session 13
Submission ID: 1628

ANTIOXIDANT CAPACITY AND PHENOLIC COMPONENTS OF


ENTEROMORPHA INTESTINALIS ALGAE

1 2 2 2 2
MIT DOKUZPARMAK , YAKUP IRIN , EMRE DOKUZPARMAK , HILAL EBRU AKIR , SEVGI KOLAYLI

ABSTRACT
Algea take an important place in the ecosystem because of their protecting effect on the
balance of the ecosystem, generating almost one-third of total photosynthetic carbon on Earth, and
also feeding aquatic creatures [1]. Enteromorpha genus is a common macrophytic green alga with a
tubular thallus found mainly in salty waters. It is often possible to observe some species in from
different types of freshwater habitats to the coastal region where photosynthetic light is available.
Enteromorpha intestinalis have a wide range of acceptable salinity, temperature and light conditions,
meaning that it can be found frequently. Enteromorpha intestinalis have approximately 20-26%
protein, some oil ,and 19-23% mineral. They contain low level of sodium and high level of iron and
calcium. The level of vitamin B and vitamin A in Enteromorpha intestinalis is higher than that of most
plants [2]. In this study, Enteromorpha intestinalis that were gathered from Trabzon-Srmene in
March (2017) were used. Antioxidant activity of methanolic extract of the algae and phenolic profile
were investigated. As antioksidant parameters, total amount of phenolic components, 2,2-diphenyl-1-
picrylhydrazile (DPPH) and Iron(III) reduce/antioxidant capacity (FRAP) were performed [3][4][5].
The total amount of polyphenol in Enteromorpha intestinalis was seen as 240 mg GAE/100g. Via
Reversed phase-High Performance Liquid Chromatography( RP-HPLC-UV) and liquid- liquid
extraction, phenolic components in Enteromorpha intestinalis were analyzed. The amount of gallic
asid, p-OH benzoic asid, vanilic asid, coumaric asid, epicatechin, rutin, daidzein, and luteolin were
determinated. Protocatequic acid, catechin, caffeic acid, syringic acid, ferulic acid, and t-cinnamic acid
were not determinated. As results of the study, it is seen that Enteromorpha intestinalis have
considerable amount of phenolic components and high level of antioxidant capacity. References; 1.
zdemir, N., & Erkmen, J. (2013). Yenilenebilir biyoplastik retiminde alglerin kullanm. Karadeniz
Fen Bilimleri Dergisi, 3(8): 89-104. 2. Nisizawa, K. (1987). Preparationand marketing of seaweeds as
foods. Food and Agriculture Organization of the United Nations, pp. 147-189. 3. Benzie, I.F.F., Strain,
J. J., In Methods in Enzymology, 299, 1527, 1999. 4. Apak, R., Gl, K., zyrek, M., Karademir,
S. E., J. Agr. Food Chem., 52, 7970-7981, 2004. 5. Yu, L., Haley, S., Perret, J., Harris, M., Vilson, J.,
Qian, M., J. Agr. Food Chem, 50, 16191624, 2002.

KEYWORDS
Algea, Enteromorpha intestinalis, Antioxidant, phenolic

Corresponding Author: MT DOKUZPARMAK, umitdokuzparmak@gmail.com


1
KARADENZ TEKNK NVERSTES, BALIKILIK TEKNOLOJS, TRABZON
2
KARADENZ TEKNK NVERSTES, KMYA BLM, TRABZON

1639
Poster Session 13
Submission ID: 1629

USE OF AROMATIC AND MEDICINAL PLANTS BY LOCALS IN


SOUTH ASIA

1 2
HILAL SAHIN-NADEEM , SAID NADEEM

ABSTRACT
Plants, both aromatic and medicinal, have been used in different forms by humans throughout
the history. Various civilizations have used these plants in different forms; single or a formulation. In
Indo-Pak, they are used in the form of Yunani medicine, Ayurveda and other folk medicines. In
Ayurveda medicinal system, the formulation may be derived from roots, leaves, seeds, fruits and barks
of various plants. Purified opium is used in eight Ayurvedic preparations to cure diarrhea, dysentery,
as well as to increase the sexual and muscular ability. Yunani medicinal system is based on the
teaching of Greek physicians Hippocrates and Galen. This medicinal system is mostly used in Pakistan
and muslim cultured central Asia as an alternative to chemotherapy. This system was practiced in
Mughal India for treatments of different diseases. Various plant extracts are prepared in the form of
syrups and pastes for oral administration. In addition to the medicinal plants, there are various locally
prepared perfumes in the market that are used in the religious activities by Muslims and Hindus.

KEYWORDS
Aromatic plants, Yunani medicines, Ayurvedic medicines, south asia, folk medicines

Corresponding Author: HLAL SAHN-NADEEM, hilal.nadeem@adu.edu.tr


1
ADNAN MENDERES UNVERSTY
2
MULA SITKI KOMAN UNVERSTY

1640
Poster Session 13
Submission ID: 1631

FOLK MEDICINAL AND AROMATIC PLANTS OF TOKAT

1 1 1 1
SEMRA TOPUZ , MUSTAFA BAYRAM , CEMAL KAYA , MEHMET GLDANE

ABSTRACT

D
Tokat which take part in Karadeniz region, is flanked by Samsun to the north, Amasya to the
northwest, Yozgat to the southwest, Sivas to the southeast, and Ordu to the northeast. Yeilyurt and
Sulusaray districts of Tokat remain in Central Anatolia Region. Tokat is located between 39 51'- 40

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55' north latitudes and 35 27'- 37 39' east longitudes. Surface area of Tokat is 10,071 km2 and
elevation from sea level of agricultural land varies between 230 m and 1500 m. Climate type of Tokat
has transition characteristic. Tokat has considerably rich flora of medicinal and aromatic plants due to
convenient of climate and soil characteristics. Some of the medicinal and aromatic plants grown in
EN
Tokat are peppermint (Mentha piperita L.), thyme (Thymus vulgaris), basil (Ocimum basilicum L.),
sage (Salvia officinalis), madmak (Polygonum cognatum Meissn), sorrel (Rumex acetosella), yarrow
(Achillea filipendulina), couch (Agropyron repens), mahaleb cherry (Prunus mahaleb), hollyhock
(Alcea rosea ), wormwood (Artemisia absinthium), daisy (Bellis perennis), lavandula (Lavandula
spp.), echinacea (Echinacea spp.), parsley (Petroselinum crispum), garlic (Allium sativum) haw
ES
(Crataegus aronia ), herba equiseti (Equisetum arvense ), fumitory (Fumaria officinalis), tiptons weed
(Hypericum perforatum), mallow (Malva sylvestris L.), caper (Capparis spp.), lemon balm (Melisa
officinalis L.), rosehip (Rosa canina), holunder (Sambucus nigra), butterbur (Tussilago farfara), nettle
(Urtica sp.), mullein (Verbascum pyroliforme), fenugreek (Trigonella foenum-graecum L.) coriander
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(Coriandrum sativum L.), poppy (Papaver somnuferum L.), sorb (Sorbus L.), linden (Tilia L.), black
sesame (Nigella sativa), cornelian cherry (Cornus mas L.), goji berry (Lycium barbarum), jujube
(Ziziphus zizyphus), etc. Increasing interest of consumers in health care, herbal medicines and organic,
natural foods reawaken medicinal and aromatic plants in the world. Medicinal and aromatic plants can
used against high blood pressure, kidney ailments, rheumatism, urinary system diseases stomach
ailments, diabetes, anaemia etc in folk remedy. For instance, Madmak (Polygonum cognatum Meissn)
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is widespread in agricultural areas or non-agricultural areas such as cropland boundaries, roadsides,


slopes and the cliffs in Tokat. Besides being consumed as food, Madmak is used diuretic and againist
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nephrolithiasis and diabetes mellitus in folk remedy. Medicinal and aromatic plants which has wide
field, are used mainly at pharmaceutical, cosmetic, food and chemical. There are some private
establishment on medical and aromatic plants in Tokat and its region. Studies for improving the
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cultivation of medicinal and aromatic plants are being carried out in Tokat.

KEYWORDS
Folk medicine, aromatic plant, medicinal plant, Tokat

Corresponding Author: SEMRA TOPUZ, semra.topuz@gop.edu.tr


1
GAZOSMANPAA NVERSTES, MHENDSLK VE DOA BLMLER FAKLTES, GIDA MHENDSL
BLM

1641
Poster Session 13
Submission ID: 1632

USE OF SEVERAL MEDICAL AROMATIC HERBS WITH ANTI-


OXIDATIVE AND ANTI-MICROBIAL EFFECT IN FOODS

1 1
FADME SEYREKOLU , SULTAN ACUN

ABSTRACT
Medical and aromatic herbs and the products such as essential oils obtained from those herbs
are widely used in medicine, cosmetic, and food industries, as well as they meet the food requirement
of humans in our country and throughout the world. Various antimicrobial and anti-oxidative materials
used in order to maintain the physical, chemical, and biological properties of foods may be exposed to
various sorts of deterioration during the storage period. Since it was determined that the use of
synthetic preservatives causes various diseases, their use in food was limited. In food industry, the
researches on natural materials to be used in order to prevent the oxidation and microorganism-origin
deteriorations were initiated. In such studies, many of medical and aromatic herbs were found to
contain high level of antimicrobial and antioxidant effects. It was determined that the herbs such as
thyme, sage, rosemary, black sesame, ginger, and marjoram were found to have antimicrobial effect on
microorganisms such as E. coli, L. monocytogenes, and S. aureus. Moreover, the essential oil
components such as Carvacrol p-cymene were found to have preventive effect on lipid peroxidation.
Prevention of oxidation positively affects maintaining the sensorial qualities of foods such as taste and
color, and this contributes to the higher quality of products to be served to customers after a storage
period. The medical and aromatic herbs being used may also be utilized in order to flavorthe foods.
Furthermore, some of the plants might be used as tea. In performed studies, the bee balm plant used as
additive in food industry was found to have antioxidant effect on gels, fruit salads, and cold beverages
and to neutralize the free radicals. Beside the use of medical and aromatic herbs in foods, they are also
used in feeding the animals, and they were reported to contribute to increasing the quality of layer
hens and broilers. In recent years, when the use of natural materials in food products instead of using
synthetic antioxidant or antimicrobial agents increased, these natural herbs having positive effects on
human health should be corroborated and encouraged via the researches.

KEYWORDS
Antioxidant, antimicrobial, oxidation.

Corresponding Author: FADME SEYREKOLU, fadime.tokatli@amasya.edu.tr


1
AMASYA NVERSTES

1642
Poster Session 13
Submission ID: 1633

THE ROLE OF NUTRACEUTICALS ON HEALTH

1 1 1 1
SEMRA TOPUZ , MUSTAFA BAYRAM , CEMAL KAYA , MEHMET GLDANE

ABSTRACT

D
The nutraceutical term is combination of nutrition and pharmaceutical words.
Nutraceuticals which have strong physiological functions and bioactivitie, can protect health and
restrain some diseases, is regulated as drug, dietary supplement, food ingredient or food. They show

TE
protective or physiological benefits against chronic diseases, but they are not considered as medicines.
Gamma-aminobutyric acid (GABA), hyaluronic acid (HA), glucosamine (GlcN), N-acetylglucosamine
(GlcNAc), glutathione (GSH), alpha-ketoglutaric acid (KG), vitamin B12, folate, riboflavin,
carotenoids (-carotene, lycopene, lutein, zeaxanthin etc.), glutathione (GSH), oleic acid,
EN
docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), arachidonic acid (ARA), conjugated
linoleic acids (CLAs), -linolenic acid, coenzim Q10, glucan, ascorbic acid, caffeic and gallic acid,
catechins, phytosterols, minerals (zinc, calcium etc.), resveratrol, quercetin, oleuropein,
hydroxytyrosol, capsaicin, inulin, fructooligosaccharides (FOS) can be given as example of
nutraceuticals. Nutraceutical have shown therapeutic effects. For instance, caretenoids have capacity
ES
to quench oxidative radicals, They exhibit anticancerogenic effect. Lycopene which reduces the risk of
prostate, breast, digestive tract, bladder, skin and cervix cancer, is found tomatoes, watermelons and
red grapefruits. Phytosterols reduce serum cholesterol levels in humans. When foods containing -
glucan is consumed, blood cholesterol, LDL cholesterol levels and the risk of coronary heart disease
PR

are reduced. Hyaluronic acid which extracted from animal tissues, stimulate fibroblast proliferation
and collagen synthesis. CLAs reduces plasma lipoproteins, strengthens immunity and inhibits cancer
cells. -KG induces procollagen and protein synthesis, regulates blood sugar level, helps treated to
nervous system diseases and acute lung injury. Nutraceuticals have many more effects such as
hypolipidemic, antiinflammation, antiaging, antidepression, antiulcer, immunity booster, detoxifier,
etc. Nutraceuticals are usually obtained by chemical synthesis and extraction from natural sources.
T

Microbial synthesis is more convenient for nutraceutical production due to the fact that chemical
synthesis and extraction from natural sources have some disadvantage such as requirement high
O

energy, occurrence of toxic by-products. GRAS (Generally Regarded As Safe) strains are used for
microbial synthesis of nutraceuticals. Lactic acid bacteria, propionibacteria, Saccharomyces cerevisiae,
Yarrowia lipolytica, Spirulina from GRAS strains used production of common nutraceuticals such as
N

hyaluronic acid, trehalose, folate, riboflavin, carotenoids, conjugated linoleic acids (CLAs), -linolenic
acid, gamma-aminobutyric acid (GABA). Nutraceuticals are generally considered safe, but they can be
harmful if taken at high doses. Morover, People which posses special conditions such as chronic
patients, pregnants should consult their doctor or pharmacist before using nutraceuticals.

KEYWORDS
Nutraceutical, nutrition, bioactivitie, health.

Corresponding Author: SEMRA TOPUZ, semra.topuz@gop.edu.tr


1
GAZOSMANPAA NVERSTES, MHENDSLK VE DOA BLMLER FAKLTES, GIDA MHENDSL
BLM

1643
Poster Session 13
Submission ID: 1634

AN ETHNOBOTANICAL STUDY ON THE HERBALISTS IN TRABZON

1 ! 1 1
ZGR EMINAAOLU , HAYAL AKYILDIRIM BEEN , ELIF AYGN , SONGL ZKAN

ABSTRACT
The study area was in Trabzon province which has a rich structure for ethnobotany and
floristic studies with its history and cultural values. The study was conducted with data of
questionnaire performed in urban, 10 herbalist and local people used this herbalist in Trabzon. The
study aimed to reveal the utilization of medicinal and aromatic plant of urban and rural people. The
rates of plant utilization, the plants they use and the problems they have encountered and their
suggestions were determined. Information regarding latin name, public name, medicinal and other
uses, opinion and problems were collected with this questionnaires. Demographic characteristics of
participants also recorded. As a result of this study, we took an inventory of these plants, classified the
plant species into their respective families. The plants with the most sales are, linden, carob, rosehip,
daisy, corn tassel, nettle, sage and ginger. It was determined that local people use more medicines and
they prepare their herbal medicines by themselves. Native people used medicinal plants most
frequently for the treatment of sedative problem, diuretic, constipation problem, diarrhea, blood
pressure disorders, respiratory tract problem and intestinal disorders. This research therefore,
specifically determined the ethnobotany of plants for reproductive health conditions;

KEYWORDS
Herbalist, Ethnobotany, Trabzon, Medicinal, Aromatic

Corresponding Author: ELF AYGN, trelifaygun@gmail.com


1
ARTVN CORUH UNVERSTY, FACULTY OF FORESTRY, DEPARTMENT OF FOREST ENGNEERNG, ARTVN

1644
Poster Session 13
Submission ID: 1635

THE USE OF ST. JOHNS WORT EXTRACT IN PRODUCTION OF


PROBIOTIC AYRAN

1 2 1
FADME SEYREKOLU , HASAN TEMZ , SULTAN ACUN

ABSTRACT
Use of St. Johns wort in depression and treatment of cancer and diseases originating from
certain viruses increases its use value. Although it is widely used for medical purposes, its use in food
products is not that wide yet. Given the literature, the use of this plant in foods is very limited. Total
phenolic matter content of the extract of this St. Johns wort (Hypericum perforatum L.) used in this
study was determined using various solvents, and the values obtained were compared. Moreover, some
of the characteristics of probiotic ayrans prepared using St. Johns wort extract (Hypericum
perforatum L.) were analyzed. In this study, 3 types of ayrans were prepared by adding 3
concentrations of St. Johns wort extract (0.1, 0.01, and 0.005%). While preparing the extracts, total
phenolic content was 677.85 g/kg GAEin case of using ethanol as solvent and 656.6 g/kg GAE in case
of using methanol. In this study, the effects of addition of St. Johns wort extract into the ayrans on
titration acidity, pH, dray matter content, taste/odor, and general hedonic scale scores were examined.
According to the results obtained, it was determined that, of the probiotic ayrans, dry matter content of
Ayran A (added with 0.1% St. Johns wort extract ), Ayran B (added with 0.01% St. Johns wort
extract ), and Ayran C (added with 0.005% St. Johns wort extract ) to be 12.0301%, 11.9586%, and
11.9511%, while the acidity values were found to be 0.648, 0.594, and 0.639, respectively. pH values
were also determined to be 5.46, 5.64,5.63. While statistically significant differences were observed in
acidity and pH parameters, no significant difference was found in dry matter content (p<0.05). From
the aspect of taste-odor and hedonic scale scores, the samples added with 0.005% extract were
appreciated at most. The use of this plant, use of which is difficult and not preferred in general, can be
increased by using it in food products appreciated by people. As in this study, its extract can be made
in beverages such as ayran that is widely consumed by young individuals, and thus a new, alternative
and healthy product can be obtained. Use of such a plant in a food that we widely consume in our
daily lives may improve its usability.

KEYWORDS
Probiotic ayran; St. Johns wort ; phenolic.

Corresponding Author: FADME SEYREKOLU, fadime.tokatli@amasya.edu.tr


1
AMASYA NVERSTES
2
ONDOKUZ MAYIS NVERSTES

1645
Poster Session 13
Submission ID: 1637

MICROWAVE ASSISTED EXTRACTION OF POMEGRANATE PEEL

1 1
HACER EN , ARIF CAN KILI

ABSTRACT
The pomegranate peels are rich in terms of ellagitannins which are valuable pharmacologic
components (ellagic acid, gallic acid, punicalin and punicalagin (A and B)). It can be obtained these
chemicals with pomegranate peel extraction, as well as it can also solve waste problem of
pomegranate processing plants. In this study, pomegranate peels were extracted by microwave assisted
extraction method to investigate the effect of extraction time, solvent /solid ratio, solvent type and
ratio and particle size on extraction yield. Ellagic acid in the extract was determined by HPLC.
Experimental studies are under way.

KEYWORDS
pomegranate peel, microwave assisted extraction

Corresponding Author: HACER EN, hacericen@osmaniye.edu.tr


1
OSMANYE KORKUT ATA NVERSTES

1646
Poster Session 13
Submission ID: 1638

OBTAINING LEMON PEEL OIL WITH SOLVENT-FREE


MICROWAVE ASSISTED EXTRACTION

1 1 1
HACER EN , HAVVA MUMCU IMEK , HANDE NUR ERGEN

ABSTRACT
Lemon peel which is food and beverage production processes' waste, includes many
pharmacologic components such as fatty acid esters, phenols, coumarin derivates such as osthole,
hexadecane and squalene, terpene derivatives. The aim of this study is obtaining lemon peel oil with
solvent-free microwave assisted extraction which is eco-friendly. The effects of microwave power and
extraction time are investigated. The most extraction yield as 0,128 g oil/g lemon peel were obtained
360 W microwave power and 12 minutes extraction time.

KEYWORDS
lemon peel, solvent-free microwave assisted extraction

Corresponding Author: HACER EN, hacericen@osmaniye.edu.tr


1
OSMANYE KORKUT ATA NVERSTES

1647
Poster Session 13
Submission ID: 1640

NEXT GENERATION DNA MARKERS IN SALVIA L. FOR


ENGINEERING OF HIGH-QUALITY PLANTS WITH ENHANCED
ACTIVE INGREDIENTS

1 2 3
MEHMET KARACA , AYE GL NCE , ADNAN AYDIN

ABSTRACT
An ideal DNA maker system is expected to have: (i) high level of polymorphism, which is the
simultaneous occurrence of allelic variability for a trait within the same population; (ii) co-dominant
inheritance to allow discrimination of homozygote and heterozygote locus; (iii) frequent occurrence in
a genome to assay more genomic regions or genes; (iv) selective neutral to environmental conditions,
developmental stage of the organism or management practices; (v) easy access (availability); (vi) easy,
sensitive and fast assay; (vii) high reproducibility within and between experiments and (viii) easy
exchange of data between laboratories. The second and third generation sequencing (NGS) methods
greatly affected the ways of DNA marker development and utilizations. Among the second generation
DNA sequencing method, 454 pyrosequencing, Solexa deep RNA sequencing (RNA-seq), Illumina
RNA-seq have been utilized to identify genes involved in pathways of secondary metabolism in Salvia
L. Data obtained from these experiments are used to aid in the selection and engineering of high-
quality plants with enhanced active ingredients. These data obtained in these experiments have also
been used DNA marker development studies. Several emerging and established next generation
approaches including restriction site-associated DNA sequencing (RAD-seq), specific length amplified
fragment (SLAF) sequencing and genotyping by sequencing analyses are breakthrough technologies
that enable scientists to efficiently discover and genotype large amounts of genetic information, in any
plant species. Genotyping by sequencing seems to have advantages over to RAD-seq and SLAF-seq
techniques in terms of sample preparation, amplification, analyses and reduced cost. Next generation
DNA marker techniques could be used in single nucleotide polymorphism and microsatellite
identification, genome wide association studies, construction of high-density genome maps,
phylogenetics studies, identification of candidate genes and genetic linkage analysis. In comparison to
conventional DNA markers such as RFLP, CAPS, SCAR, SNP, SSCP and SSR, next generation DNA
markers do not need sequence information, many sample handling, PCR and purification steps. They
have efficient bar-coding system and capable of high-throughput, mass parallelisation of simultaneous
marker discovery and genotyping. Also these techniques do not require high cost per marker analysis.
The genus Salvia L. consists of about 1000 species, most of which have not been genetically
characterized at the DNA level. Sufficient DNA markers in the genus have not been developed, and
the most of species in the genus Salvia do not have high-density genetic linkage maps. Thus next
generation DNA marker technologies could be very helpful for genetic studies of Salvia. Among these
next generation marker technologies, SLAF-seq has already used in Salvia miltiorrhiza Bunge, also
known as danshen. In a previous study researchers extracted from two parents and their 96 F1
individuals of Salvia and subjected to high-throughput SLAF-seq studies. A total of 155.96 Mb of data

Corresponding Author: ADNAN AYDIN, aydina@akdeniz.edu.tr


1
AKDENZ NVERSTES

1648
contained 155,958,181 pair-end reads. The final linkage map consists of 5,164 SLAFs in 8 linkage
groups and spans 1,516.43 cM, with an average distance of 0.29 cM between adjacent markers. In this
study, step by step comparison of next generation DNA marker technologies are made and advantages
and disadvantages of these technologies in comparison to old and next generation methods are
discussed.

KEYWORDS
DNA markers, marker development, Next Generation Sequencing, Salvia, SNP

1649
Poster Session 13
Submission ID: 1641

MICRONUTRIENT MINERAL COMPOSITION OF PLANTAGO


ANATOLICA TUTEL & R. MILL.

1 1
MUZAFFER MKEMRE , ABDULLAH DALAR

ABSTRACT
Plantago anatolica Tutel & R. Mill. is an endemic medicinal plant species which is growing in
Eastern Anatolia Region and extensively used in the treatment of various disorders by indigenous
people. Plant species belong to Plantago genus have been extensively used in folk medicine worldwide
and in our country. Although the phytochemical composition and various health attributing properties
of these species were partly investigated, there is no or relatively limited study in the scientific
literature regards to Plantago anatolica. Within this study, investigation of Plantago anatolica plant in
the respects of micronutrient mineral composition was targeted. Plant materials were extracted by
using 4 different extraction methods: sequential extraction (using different organic solvents
sequentially including n-hekzan, chloroform, ethyl-acetate, acetone, ethanol and pure water), infusion,
decoction and pure water extraction. Micronutrient mineral compounds were analyzed by Inductively
Coupled Plasma Optical Emission Spectroscopy (ICP-OES) and Atomic Absorption Spectroscopy
(A.A.S). Within this presentation the amount of Ag, As, B, Ba, Be, Cd, Co, Cr, Cu, K, Mg, Mn, Mo,
Ni, Pb, Sb, Se, Si, Ti, V, Zn, Na, Ca and Fe of different lyophilized extracts obtained from plant
materials will be discussed with the updated scientific literature.

KEYWORDS
Plantago anatolica, minerals, extraction,

Corresponding Author: MUZAFFER MKEMRE, mukemre.muzaffer@gmail.com


1
YZNC YIL NVERSTES

1650
Poster Session 13
Submission ID: 1642

EFFECT OF AIR DRYING TEMPERATURE ON DRYING KINETICS,


ANTIOXIDANT CAPACITY AND TOTAL PHENOLIC CONTENT OF
CELERY LEAVES

1 2 2
SENEM TFEKI , EZGI ZGREN , SAMI GKHAN ZKAL

ABSTRACT
Effect of air drying temperature on drying kinetics, antioxidant capacity and total phenolic
content of celery leaves Senem TFEK1, Ezgi ZGREN2, Sami Gkhan ZKAL2* 1
Pamukkale University, Vocational School of Acpayam, Department of Food Processing, Denizli,
Turkey, 1 Pamukkale University, Faculty of Engineering, Department of Food Engineering, Denizli,
Turkey, *Presenting Author: S.G. ZKAL (sgozkal@pau.edu.tr) Drying of spices and herbs is a
common method for producing of flavoring ingredients. Celery leaves have characteristic aroma and
odor that makes them a potential flavoring agent. It also have medicinal usage against asthma,
bronchitis, and rheumatism. In this study, it was aimed to investigate the effect of drying temperature
on dehydration kinetics, antioxidant capacity and total phenolic components of celery leaves. For this
purpose, celery leaves dried in a hot air oven and drying temperature was chosen as 40 C, 50 C, and
60 C. Drying temperature effected drying rate of celery leaves and shortened drying time
significantly. Time to reduce moisture ratio of samples to 0.05 for drying experiments at 40C, 50 C
and 60 C air temperatures was found as 140, 80 and 60 minutes, respectively. Five different thin layer
drying models were fitted to experimental drying data. Page and Modified Page models gave the
higher R2 (0.9903-0.9982) and lower 2 (0.000089-0.001073) and RMSE (0.002579-0.007279)
values, and therefore these models were found to be the most suitable models for describing drying
characteristics of celery leaves. The transport rate of water during drying was described by effective
moisture diffusivity (Deff) based on Fick's equation and its value was found as 2.74 10-10, 4.56
10-10 and 7.30 10-10 m2/s for samples dried at 40 C, 50 C and 60 C, respectively. The values
were in agreement with the general range of 10-12 10-8 m2/s for drying of biological material. Dried
celery leaves were analyzed for antioxidant activity and total phenolic content using 1,1-diphenyl-2-
picrylhydrazyl (DPPH) and Folin-Coiocalteu methods. Both antioxidant activity and total phenolic
content of the samples dried at 40C had higher values. Results suggest that there is no significant
(p>0.05) difference in antioxidant activity and total phenolic content between dried samples.
Antioxidant activity and total phenolic content showed a slight reduction for 50C dried sample in
comparison with 60C sample. Therefore, 60C can be selected as a drying temperature for celery
leaves by considering the short drying time and negligible losses of antioxidant activity and total
phenolic content.

KEYWORDS

Corresponding Author: SAM GKHAN ZKAL, sgozkal@pau.edu.tr


1
PAMUKKALE UNVERSTY, VOCATONAL SCHOOL OF ACIPAYAM, DEPARTMENT OF FOOD PROCESSNG
2
PAMUKKALE UNVERSTY, FACULTY OF ENGNEERNG, DEPARTMENT OF FOOD ENGNEERNG

1651
Celery leaves, air drying temperature, antioxidant activity, total phenolic content,
mathematical modelling

1652
Poster Session 13
Submission ID: 1646

ANTIMICROBIAL ACTIVITY SCREENING OF FRANGULA ALNUS

1 2 3 4
ALI YETGN , KEREM CANLI , ILGAZ AKATA , ERGIN MURAT ALTUNER

ABSTRACT
According to traditional medicine of America and Europe knowledge, some species of
Frangula genus are widely used. Frangula alnus bark extracts have anticancer, antifungal, antioxidant,
antiviral and antibacterial activity, so it is significant for official and folk European medicine. Barks of
this medical plant investigation were applied against 17 bacteria and 1 fungi by using disk diffusion
method. These microbial strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella,
Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida geniuses. Twelve of them are
standard species and they are important for exact determination of antimicrobial potential. 6.32 and
21.06 mg samples were prepared by using ethanol extraction method. The results were presented that
F. alnus has antimicrobial activity against all tested microbial strains. Six of them have high
susceptibility (higher than 15 mm); nine of them have moderate susceptibility (14-10 mm) and only
three of them have low susceptibility (9-7 mm).

KEYWORDS
Frangula alnus, medicinal plant, antimicrobial activity, disc diffusion method, ethanol extract.

Corresponding Author: AL YETGN, ali1992yetgin@gmail.com


1
DEPARTMENT OF BOTECHNOLOGY, INSTTUTE OF ENGNEERNG AND SCENCE, IZMR INSTTUTE OF
TECHNOLOGY, IZMR, TURKEY
2
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, DOKUZ EYLL UNVERSTY, IZMR, TURKEY
3
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, ANKARA UNVERSTY, ANKARA, TURKEY
4
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE AND ARTS, KASTAMONU UNVERSTY, KASTAMONU,
TURKEY

1653
Poster Session 13
Submission ID: 1647

COMPARATIVE EVALUATION OF THE INHIBITORY EFFECT OF


SOME ESSENTIAL OILS ON ACETYLCHOLINESTERASE ACTIVITY
IN HUMAN LUNG CARCINOMA (A549) CELLS

1 1 1 1
EMINE TORAMAN , YELIZ DEMR , HAMID CEYLAN , ORHAN ERDOAN

ABSTRACT
Current therapeutic strategies for Alzheimer Disease (AD) focus on increasing acetylcholine
(Ach) bioavailability at the synapse. Therefore, revealing enzyme response against therapeutic agents
that alter acetylcholinesterase (AChE) activity is extremely important for orienting treatment
strategies. Today, there is an increasing considerable interest in the use of essential oils as alternative
therapeutic. In this study, we investigated the biological activity (acetylcholinesterase inhibition) of
some essantial oils (Helichrysum italicum and Rosmarinus officinalis) in A549 cells. For this purpose,
human lung cancer cells (A549) were treated with different concentrations of Helichrysum italicum (
1-10 l/well) and Rosmarinus officinalis (1-5-10 l/well) essential oils. Rosmarinus officinalis
essential oil was found the active AchE inhibitor followed by Helichrysum italicum essential oil.
Decreased AChE activities in A549 cells were observed in a dose-dependent manner. We aim that the
results to be obtain from this study is considered to be contribute for further researchs that may result
in the annotation of therapeutic drugs improvement or treatment strategy for AChE related diseases.

KEYWORDS
A549, Acetylcholinesterase, Essential oil, Inhibition

Corresponding Author: HAMD CEYLAN, hamid.ceylan@atauni.edu.tr


1
ATATRK NVERSTES

1654
Poster Session 13
Submission ID: 1649

COMPARISON OF ANTIOXIDANT CONTENTS OF DIFFERENT


SOLVENT EXTRACTS FROM A FRESHWATER ALGAL SPECIES

1 2 3
BLENT AKAR , ZEYNEP AKAR , BLENT AHN

ABSTRACT
Algae, including both microscopic and macroscopic organisms are photosynthetic organisms
that develop in all environments except extreme drought place. Especially, they are the main source of
primer productivity in all aquatic ecosystems. Generally, to supply both medicine and food for people
are used organisms which easily cultivated and accessed. However, in recent times due to the
increasing needs, use of algae having rich biological diversity are also increase in these areas. Algae
are used in many industries such as food, medicine, cosmetics, construction, textile, paper, biofuels
and microbiological media. Microspora sp. (Chlorophyta = Green Algae) was collected from localised
a lake at an altitude of 2730 meters in the district of Torul in Gmhane province in Turkey. Algal
samples were collected at a depth of 0-0.5 m from the surface of the lake during the summer when the
biomass was high (algal bloom present). The obtained sample was washed several times with distilled
water, dried, then ground, and methanol, ethanol, acetonitrile, acetone and ethyl acetate extracts were
prepared. Antioxidant activity of each extract was determined by three different antioxidant assay
methods: DPPH cleaning, iron (III) reduction / antioxidant power (FRAP) and copper (II) reducing
antioxidant capacity (CUPRAC). When the calculated test results were compared, similar results were
observed in all three methods. While the highest antioxidant activity was determined in methanol
extract (31.15%), the lowest activity was measured in acetonitrile extract (8.97%). In conclusion,
Microspora sp. has antioxidant activity in different solvents. Therefore, studies can be made for its use
in many fields, such as mainly food and medicine.

KEYWORDS
Microspora sp., DPPH, FRAP, CUPRAC, Antioxidant

Corresponding Author: BLENT AKAR, akarblnt@gmail.com


1
GMHANE UNVERSTY, GMHANE VOCATONAL SCHOOL, DEPARTMENT OF FORESTRY, 29100,
GMHANE, TURKEY
2
GMHANE UNVERSTY, FACULTY OF ENGNEERNG AND NATURAL SCENCES, DEPARTMENT OF
GENETCS AND BOENGNEERNG, 29100, GMHANE, TURKEY
3
KARADENZ TECHNCAL UNVERSTY, FATH EDUCATON FACULTY, DEPARTMENT OF BOLOGY, 61300,
TRABZON, TURKEY

1655
Poster Session 13
Submission ID: 1650

ANTIMICROBIAL ACTIVITY SCREENING OF RHYNCHOSTEGIUM


MEGAPOLITANUM

1 2 3 4
ALI YETGN , KEREM CANLI , ILGAZ AKATA , ERGIN MURAT ALTUNER

ABSTRACT
In North America, Europe and China, bryophyte have been used as medicinal purpose since
400 years ago. Although antifungal and antibacterial potential of some bryophyte species are
determined, most of them are not investigated. Broad range antimicrobial activity of bryophyte species
is became a crucial, therefore related research must be applied. In this research, antimicrobial potential
of Rhynchostegium megapolitanum against a wide range of microorganisms was analyzed. The
analyses contain ethanol extract of R. megapolitanum against 17 bacterial and 1 fungal species. These
microbial strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria,
Pseudomonas, Salmonella, Staphylococcus and Candida geniuses. Twelve of them are standard
species and they are important for exact determination of antimicrobial screening. 0.34, 0.68 and 1.13
mg samples were prepared and their activity was analyzed by using agar diffusion method. Our
present study has shown that R. megapolitanum has antimicrobial activity against eight of the studied
species. Antimicrobial potential of R. megapolitanum are being investigated for the first time.

KEYWORDS
Rhynchostegium megapolitanum, bryophyte, antimicrobial activity, disk diffusion method,
ethanol extract.

Corresponding Author: AL YETGN, ali1992yetgin@gmail.com


1
DEPARTMENT OF BOTECHNOLOGY, INSTTUTE OF ENGNEERNG AND SCENCE, IZMR INSTTUTE OF
TECHNOLOGY, IZMR, TURKEY
2
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, DOKUZ EYLL UNVERSTY, IZMR, TURKEY
3
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, ANKARA UNVERSTY, ANKARA, TURKEY
4
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE AND ARTS, KASTAMONU UNVERSTY, KASTAMONU,
TURKEY

1656
Poster Session 13
Submission ID: 1651

USAGE OF NUTRITIONAL SUPPLEMENTS IN TURKEY

1 1
NAZAN AKTA , EBRU BAYRAK

ABSTRACT
Dietary support and nutrient enrichment for the treatment and prevention of nutritional
problems are interventional methods commonly used in both developed and developing countries.
Nutritional supplements are defined as vitamins and minerals can be used in the form of pills,
capsules, syrups in quantities corresponding to high doses. These generally have a wide array of
including vitamins, minerals, amino acids, essential fatty acids, fibre, various plants and their extracts.
The purpose of this study was to evaluate the use of nutritional supplements according to age, gender,
settlement and NUTS 12 regions in Turkey. In this review, data was obtained from Turkey Nutrition
and Health Survey (TNHS)-2010 about the use of nutritional supplements. At the TNHS-2010, the
participants were asked about the nutritional supplements they had used in the last week; were
evaluated according to the variables such as age, gender, settlement and NUTS 12 regions. In Turkey,
23.9% of children aged 0-5 use at least one nutritional supplement. When the use of nutritional
supplements was examined according to the region, it was seen that the highest usage rate was in
Western Marmara (34.1%) and the least used region was Middle East Anatolia (15.6%) for this age
group. The most commonly used nutritional supplements for children in this age group were vitamin D
and iron supplements. The rate of use of food supplements in children aged 6-11 years was low (3.1%)
and it was determined that they did not use vitamin D, folic acid and iron as nutritional supplements.
When the age of 12 years and over were examined, the most commonly used nutritional supplements
were reported as vitamin B12 and zinc as the least used. When evaluated in terms of gender, it was
determined that the highest amount of calcium (3.4%) and vitamin B12 (3.3%) were used in women,
and vitamin B12 (1.6%) was the highest in men in Turkey. When nutritional supplements are used, the
amount of consumption and type of these supplements must be determined by the experts.
Determining the rates of these products is important at the point of setting the strategies for producers,
the state and related non-governmental organizations.

KEYWORDS
Nutritional supplements, TNHS-2010, gender, age

Corresponding Author: NAZAN AKTA, naktas@selcuk.edu.tr


1
SELUK NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1657
Poster Session 13
Submission ID: 1653

COMPARISON OF ANTIOXIDANT ACTIVITY OF BAKED AND RAW


MATERIALS OF GYROMITRA ESCULENTA

1 1 ! 1 1 1
KBRA ELIK , MEHMET ZTRK , ERHAN KAPLANER , ZAIN ULLAH , YUSUF SICAK , MEHMET EMIN DURU ,
2 3 4
ABDULSELAM ERTA , RIADH BEN MONSOUR , MEHMET HSEYIN SINGE

ABSTRACT
Comparison of Antioxidant Activity of Baked and Raw Materials of Gyromitra esculenta
Kbra ELKa, Erhan Kaplanera, Zain Ullaha, Yusuf SICAKa,b, Abdulselam ERTAc, Riadh Ben
Mansourd, Mehmet ZTRKa, Mehmet Emin DURUa E-mail: kubra_elikk@hotmail.com
aDepartment of Chemistry, Faculty of Sciences, Mula Sitki Koman niversity, Mentese-Mula,
Turkey bDepartment of Herbal and Animal Production, Kyceiz Vocational School, Mula Stk
Koman University, Koycegiz-Mula, Turkey cDepartment of Pharmacognosy, Faculty of Pharmacy,
Dicle University, Sur-Diyarbakr, Turkey dEcole Nationale d'Ingnieurs de Sfax, Route de la Soukra
km 4 - 3038 Sfax, Tunisia Abstract Gyromitra esculenta (Pers.) Fr. is delicious food consumed in
Scandinavian countries to Anatolia. Gyromitra esculenta is found in temperate coniferous forests,
especially in coniferous trees, which grow on sandy soil; It is a mushroom species that has a red-
brown cover under pine trees and sometimes poplar trees and darkens as it gets older. Locally, it is
called false morel and when consumed raw it poisons. Despite poisonous in raw form, Gyromitra
esculenta is very popular and has been consumed in Scandinavia and North America. It is sold in the
market with attached cooking instructions; and consumed in omelets, soups, salads and other common
food. Before consume, required cooking should be even necessary. The active constituent of this
mushroom exhibiting toxic effects is gyromitrine which is hydrolyzed to monomethylhydrazine during
cooking. In giromitrin hydrochloric acid and ethanol catalysis the input is converted to
monomethylhydrazine (MMH) This toxin affects liver, central nervous system and sometimes
kidneys. Because of its consumption and widely growing in Anatolia it was aimed to study the
antioxidant activity of extracts of both raw and baked materials. Gyromitra esculenta (Pers.) Fr. was
purchased from local market and divided in to two parts. One part was baked in an oven at 200C for
30 minutes. The other part was left raw. Then both cooked and uncooked materials were airdried
under shadow. The dried both samples were grinded and extracted with petroleum ether, acetone and
methanol and water, successively. The extracts were studied for their antioxidant activity using
complimentary assays; for this purpose, CUPRAC experiments were carried out based on measuring
the total antioxidant amount based on -carotene-linoleic acid chromophore method, DPPH free
radical scavenging activity method, ABTS cation radical scavenging antioxidant activity method and
copper (II) reduction capacity. For each antioxidant activity assay, the extracts were dissolved at
concentrations of 4000 ppm, 2000 ppm, 1000 ppm and 500 ppm, respectively. These solutions were
chosen as the control solution. The initial absorbance was adjusted according to each method by

Corresponding Author: KBRA ELK, kubra_elikk@hotmail.com


1
MULA SITKI KOMAN NVERSTES
2
DCLE NVERSTES
3
ECOLE NATONALE D'INGNEURS DE SFAX, ROUTE DE LA SOUKRA KM 4 - 3038 SFAX, TUNSA
4
MUGLA STK KOCMAN UNVERSTY

1658
means of a spectrophotometer. For example, the initial absorbance at 517 nm for the DPPH assay was
chosen as 490 nm for the -carotene linoleic acid decolorization method. The absorbance values of the
control solution in the cuvettes and the absorbance values of the sample substances in the test result
were compared and the results were calculated. The total phenolic content of the extracts was
determined as pyrocatechol equivalents. The results in -carotene lipid peroxidation and DPPH radical
scavenging experiments, it was shown that the gyromitra esculenta mushroom samples cooked for
each of the petroleum ether, acetone, methanol and water extracts had higher antioxidant capacity than
the raw extracts. Keywords: Gyromitra esculenta, Antioxidant activity, Baking effect
Acknowledgement: This study is supported by the TUBTAK with the Project number KBAG-
114Z635. References [1] Mat, Afife. (2000). Trkiyede Mantar Zehirlenmeleri ve Zehirli Mantarlar,
Nobel Tp Kitapevi Press, 2.Edition, Istanbul Turkey. [2] Arshadi, M., Nilsson, C., Magnusson, B.,
2006. "Gas chromatography-mass spectrometry determination of the pentafluorobenzoyl derivative of
methylhydrazine in false morel (Gyromitra esculenta) as a monitor for the content of the toxin
gyromitrin", Journal of Chromatography A, 1125(2), 229-233.

KEYWORDS
Gyromitra esculenta, Antioxidant activity, Baking effect

1659
Poster Session 13
Submission ID: 1656

THE INVESTIGATION OF ANTIOXIDANT AND ANTI-UREASE


ACTIVITIES AND PHENOLIC COMPOUNDS OF THYMUS PRAECOX
SUBSP. SKORPILII VAR. SKORPILII

1 2 2
ABDULKADIR UMAN , DUYGU TAKIN , TURGUT TAKIN

ABSTRACT
Thyme species (genus Thymus, family Lamiaceae) are well known aromatic perennial herbs
used extensively throughout the Mediterranean basin. This genus is represented by 38 species and
altogether 64 taxa, 24 of them are endemic in Turkey. Members of this genus are called kekik in
Turkish and are used as herbal tea. Several thyme species are used as medicinal remedies against a
variety of diseases as well as for aromatic, culinary, and food preservative purposes. A wide range of
biological and pharmacological properties have been reported for these species, such as antiseptic,
antitussive, expectorant, antispasmodic, and anti-inflammatory activities. These biological and
pharmacological properties have been mainly attributed to the rich essential oils contained in the
majority of thyme species as well as nonvolatile compounds including polyphenols and flavonoids
(1,2). Thymus praecox subsp. skorpilii var. skorpilii essential oil was found to contain thymol and o-
cymene as the major components (2). The aim of this study was to investigate phenolic composition
and compare evaluation of antioxidant and anti-urease activities of Thymus praecox subsp. skorpilii
var. skorpilii. In the present study, The total phenolic contents, antioxidant and anti-urease activities of
different extracts from Thymus praecox subsp. skorpilii var. skorpilii were compared. The extracts
were quantitatively analyzed for total phenolic contents using FCR assays. Antioxidant activity of the
extracts was measured using the DPPH., ABTS.+ scavenging activities and FRAP assay. The urease
inhibitory activities of the extracts were determined according to a reported method (3). The methanol
extract of Thymus praecox subsp. skorpilii var. skorpilii had the highest amounts of total phenolics
(0.0700.0001 mgGAE/mg extract) and showed the highest biological activity (ABTS radical
scavening activity: 98%; FRAP: 17.400.004 mM FeSO4/mg extract). Subsequently, we tried to
identify the major phenolic compounds in methanol extract by HPLC-DAD/ESI-Q-TOF LC/MS. The
major phenolic compounds were identified as: chlorogenic acid, luteolin 7-O-glycoside, quercetin
hexoside, apigenin 7-O-glucuronide and feruloylquinic acid. References 1.Jamali CA, Bouzidi LE,
Bekkouche K, Lahcen H, Markouk M, Wohlmuth H, Leach D, Abbah A, Chemical composition and
antioxidant and anticandidal activities of essential oils from different wild Moroccan Thymus species,
Chemistry and Biodiversity, 2012; 9 1188-1198. 2.Ozen T, Demirtas I, Aksit H, Determination of
antioxidant activities of various extracts and essential oil compositions of Thymus praecox subsp.
Skorpilii var. skorpilii, Food Chemistry, 2011; 124 58-64. 3.Ghous T, Akhtar K, Nasim FUH,
Choudhry MA. Screening of selected medicinal plants for urease inhibitory activity, Biology and
Medicine, 2010; 2 (4): 64-69.

Corresponding Author: DUYGU TAKIN, duygu.susuz@yeniyuzyil.edu.tr


1
MARMARA NVERSTES
2
STANBUL YEN YZYIL NVERSTES

1660
KEYWORDS
Thymus praecox subsp. skorpilii var. skorpilii, phenolic compounds, antioxidant anti-urease,
Q-TOF LC/MS

1661
Poster Session 13
Submission ID: 1659

SOME CHARACTERISTICS OF INSTANT TEA PRODUCED FROM


MINT (MENTHA PIPERITA L.)

1 2 2 2 2
ZEYNEP AKIT , SEMRA TOPUZ , CEMAL KAYA , MUSTAFA BAYRAM , HSEYIN AKIT

D
ABSTRACT
Mint (Mentha piperita L.) is widely consumed as herbal tea in Turkey. Mint which is a very

TE
old culture plant, take parts among the medical plants due to its biological effects such as anti-
inflammatory, cardioprotective, antioxidant, antibacterial. In this study, it was aimed to enhance
alternative methods for instant mint tea production. Instant tea powders were obtained by spray drying
and freeze drying techniques from watery extract of mint. Some physical and chemical properties of
these tea powders were investigated. Solubility in water, moisture content, yield, Hunter colour values
EN
(L*, a*, b*), total phenolic compounds and total sugar quantities were determined. The antioxidant
activity of instant tea powders were evaluated with FRAP and TEAC tests. Some individual phenolic
compounds and flavor compounds of mint tea powders were determined by HPLC and GC-MS,
respectively. Moreover, instant mint tea powders were evaluated for their sensory properties. The
ES
results were compared with traditional brewing method. Solubility in water of mint tea powders by
using freeze drying and spray drying techniques were found 96,23; 98,33 and moisture content were
found 5,56; 0,24%. Yield of tea powder produced from mint (traditional; freeze dried; spray dried)
were found 13,77; 23,53; 23,28%. Moreover, the hunter colour values of mint teas prepared by using
traditional brewing, freeze dried tea powder, spray dried tea powder were 26,04; 26,19; 26,56 L*,
PR

3,81; 4,06; 4,48 a*, -3,65; -2,45; -1,82 b*. Total phenolic content of tea produced from pepermint
(traditional; freeze dried; spray dried) were found as 12.01; 30.85; 34.24 mgGAE/100 mL and total
sugar quantities were found as 36,07; 40,98; 44,94 mgGE/100mL. FRAP activities were found as
2936,73; 3774,64; 4323,66 molTE/100 mL and TEAC activities were found as 2854,18; 3398,69;
4008,85 molTE/100 mL, respectively. Hesperidin, rosmarinic acid, linarin, didimin, nevadensin,
retusin were identified and quantified of pepermint teas by using high performance liquid
T

chromatograph (HPLC). The amount rosmarinic acid (major phenolic of mint) determined 5,73; 15,17
and 15,58 mg/100 mL, respectively. The amount of phenolic compounds obtained by spray drying and
O

freeze drying techniques are higher than traditional brewing method. However, spray dried herbal tea
powders had less flavoring agents because of heat application during drying. When the results of the
N

sensory analysis were examined, the highest average was obtained by traditional method and followed
by freeze drying techniques, spray drying, respectively. In this sense, the most important factor may be
preservation or losing of volatile aroma components during processings.

KEYWORDS
Mint (Mentha piperita L.), instant herbal tea, spray drying, freeze drying

Corresponding Author: CEMAL KAYA, cemal.kaya@gop.edu.tr


1
GMHANE NVERSTES
2
GAZOSMANPAA NVERSTES

1662
Poster Session 13
Submission ID: 1660

AMINO ACID COMPOSITIONS OF SOME IMPORTANT AROMATIC


PLANTS

1 1 1 1 1
MEHMET FATIH CENGIZ , NILDA ERSOY , TIMUR TONGUR , TANER ERKAYMAZ , ONUR BASANELEBI

ABSTRACT
The goal of the research was to determine the similarities and differences in the compositions
of amino acids in some important aromatic plants grown by Good Agricultural Practices (GAP) in
Turkey. In the study, Pimpinella anisum (anise), Nigella sativa (blackcumin), Papaver somniferum
(poppy), Coriandrum sativum (coriander) and Foeniculum vulgare (fennel) samples were examined.
Chromatographic separations of amino acids were achieved by an Hypersil Gold 100 mm x 2.1 mm x
1.9m C18 column with 5mM ammonium acetate in water: MeOH (95:5) %0.1 formic acid (A) and
MeOH (B). Separated amino acids were identified by liquid chromatography tandem mass
spectrometry (LC MSMS) without the need for derivatization. A total of 15 amino acids (arginine,
aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine,
proline, serine, threonine, tyrosine and valine) were analyzed. The method was found to be selective,
linear (r2>0.99) and precise for all of interested amino acids. The limits of the quantifications (LOQ)
were found to be in the range from 0.53 to 1.20 mg kg1. Results showed that the amino acid contents
of anise, blackcumin, poppy, coriander and fennel were found to be 2511.04, 422.54, 666.96, 594.19
and 579.05 respectively. None of the analyzed samples was contained cysteine. Threonine, histidine
and valine amino acids were detected in only anise samples. In addition, the highest concentration of
lysine, arginine, aspartic acid, proline, methionine, tyrosine, leucine + isoleucine and phenylalanine
amino acids were found in anise samples. Fennel has the highest concentration of serine. In
conclusion, anise has the most valuable aromatic plant in tested samples due to its high content of
amino acids. Therefore, it can be preferred in medicinal and healthcare treatment which aminoacid
compositions are accepted as important.

KEYWORDS
Amino acid, Aromatic plants, Composition

Corresponding Author: MEHMET FATH CENGZ, fcengiz@akdeniz.edu.tr


1
AKDENZ NVERSTES

1663
Poster Session 13
Submission ID: 1661

INVESTIGATION OF FELD CROPS AND SOME QUALITY


CHARACTERISTICS (SIDERITIS CONGESTA DAVIS ET HUBER-
MORATH) OF CULTIVATED MOUNTAINS

1 1
EMINE BLGNOLU , YKSEL KAN

ABSTRACT
This research has beenconductedunder Konya ecologicalconditionstodetermine the effect on
yeld and qualitysomecharacters of nitrogenfertilizersapplied at the differentdoses of Sideritis congesta
inMedicinal Plants laboratory and Medicinal PlantsExperimental Farm of AgricultureFaculty,
SelcukUniversty. Experiment wasdesigned and applied inrandomizedcompleteplotdesign with
threereplications in the year of 2012-2013. Accordingtoresults of thisresearh; the plantheight of
Sideritis congesta 58.66-64.33 cm, the number of floweringbranches 49.00-55.00, freshfloweringyield
446.66-623.33 kg / da, essential oil yield 0.24-0.33 % and major essential oil component ( pinene)
wasvarriedbetween. 43.245 and 48,459. The highestdrugfloweryield and essential oil
yieldformountaintea in Konya and similarecology 10 kg/da nitrogenfertizerapplication is reasonabled.

KEYWORDS
Mountaintea, Sideritis congesta, Essential oil, pinene, Fertilizer

Corresponding Author: EMNE BLGNOLU, bilginogluemine@gmail.com


1
SELUK NVERSTES

1664
Poster Session 13
Submission ID: 1663

HERBAL MEDICINES USED IN THE TREATMENT OF DIABETES IN


VAN PROVINCE (TURKEY).

1 1 1
ABDULLAH DALAR , MUZAFFER MKEMRE , SEMA SEZGIN

ABSTRACT
Diabetes is a group of metabolic diseases characterized by hyperglycaemia resulting from
defects in insulin secretion, insulin action, or both. (American Diabetes Association, 2012). The
prevalence of diabetes for all age-groups worldwide was estimated to be 2.8% in 2000 and 4.4% in
2030. The total number of people with diabetes is projected to rise from 171 million in 2000 to 366
million in 2030 (Wild et al., 2004). Commercially available antidiabetic drugs (acarbose, metformin,
miglitol and voglibose) used in the treatment and/or management of diabetes have potentially
hazardous side effects (liver problems, diarrhoea etc.). Phytopharmaceuticals are seen as
complementary medicines or an alternative to conventional medicines with fewer side effects.
Phytochemicals identified from traditional medicinal plants present an exciting opportunity for the
development of newer antidiabetic agents. Some natural herbal sources which have extraordinary
antidiabetic potential are tea polyphenols, pine bar extract, and kotalanol. Local people of Van
province have been used various medicinal plants in the treatment of diabetes for a long time.
Antidiabetic preparations have been used as valuable alternative and/or complementary agents to
conventional medicines in the province. We have detected several antidiabetic herbal medicines in this
region such as Diplotaenia sp., Ferula sp., Heracleum sp., Artemisia sp., Centaurea sp., Helianthus sp.,
Helichrysum sp., Astragalus sp. and etc. Within this presentation scientific and local names of these
plant species used in the treatment of diabetes, their preparation and utilization methods will be
presented. References: 1. American Diabetes Association., 2012. Diagnosis and classification of
diabetes mellitus. Diab. Care. 35 (Suppl.), 64-71. 2. Wild, S., Roglic, G., Green, A., Sicree, R., King,
H., 2004. Global prevalence of Diabetes: Estimates for the year 2000 and projections for 2030. Diab.
Care. 27 (5), 1047-1053.

KEYWORDS
Diabetes, Van province, herbal medicines

Corresponding Author: ABDULLAH DALAR, dalar.abdullah@gmail.com


1
YZNC YIL NVERSTES

1665
Poster Session 13
Submission ID: 1664

ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC AND FLAVONOID


CONTENTS IN SOME HERBAL TEAS

1 2 3 2
LHAMI KARATA , NUSRET GEN , RAHIME KARATA , MAHFUZ ELMASTA

ABSTRACT
Medicinal and aromatic plants are widely used as herbal teas for the prevention or remedy of
many diseases because of their high antioxidant activity and secondary metabolite contents.
Antioxidant activity and metabolite contents of plants are analyzed in different solvents and extraction
methods in scientific research; however they are widely consumed from peoples in boiling water
infusion. The aim of this study is to determine total phenolic, total flavonoid contents and antioxidant
activity of some herbal tea including, mint (Mentha piperita), lemon balm (Mellissa officinalis),
marshmallow (Althea officinalis), chamomile (Matricaria chamomilla ), green tea (Camellia sinensis),
sage (Salvia officinalis). The antioxidant activity of these six medicinal and/or aromatic plants were
evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH.), ferric reduction power (FRAP) and
2,2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid radicals (ABTS+). The samples (1 g) were
brewed in boiling water (100 ml) for 5 minutes and the extracts were used for estimation of these
parameters. The extraction method was specially selected in the form of widespread use by people.
Total phenolic content of the extracts ranged from 6.71 0.29 to 89.82 0.78 mg GAE /g DW. The
extract of Camellia sinensis exhibited the highest total phenolic content. The flavonoids concentrations
of the extracts ranged from 0.292 0,003 to 0.965 0.02 mg QUA/g DW from which the extract of
Salvia officinalis had the highest flavonoids content. Among the plants studied, Camellia sinensis
showed the highest antioxidant activity in terms of the three parameters. After the green tea, the
highest ABTS and FRAP activities were observed in Mentha piperita and Salvia officinalis
respectively, but the DPPH activity of the Salvia officinalis is higher than Mentha piperita.

KEYWORDS
Medical and aromatic plants, phenolic compounds, DPPH, ABTS, FRAP

Corresponding Author: LHAM KARATA, ilhami.karatas@gop.edu.tr


1
GAZOSMANPAA UNVERSTY, ALMUS VOCATONAL SCHOOL, TOKAT
2
GAZOSMANPAA UNVERSTY, FACULTY OF ARTS AND SCENCES, DEPARTMENT OF CHEMSTRY
3
MDDLE BLACK SEA TRANSTONAL ZONE AGRCULTURAL RESEARCH INSTTUTE TOKAT

1666
Poster Session 13
Submission ID: 1665

EFFECTS OF PLANT-DERIVED COMPONENT TRANS FERULIC


ACID, RESVERATROL AND COMBINATION ON RAT BRAIN
CANCER GLIOMA (C6) CELL LINES

1 1
NEBIYE PELIN TRKER , AYTEN BOSTANCI

ABSTRACT
Resveratrol (trans-3,5,4'-trihydrocystilbene), a polyphenolic compound, is a natural
phytochemical found in many plant species including red grapes, blueberries, peanuts, various fruits
and herbs as well as in processed products such as wine. Resveratrol, which acts as an antioxidant to
prevent DNA damage that can lead to tumor formation in healthy cells, induces the onset of apoptosis
in cancer cells. Ferulic acid (4-hydroxy-3-methoxy-cinnamic acid, FA) is a compound commonly
found in plants. It is synthesized from phenylalanine or L-tyrosine and is formed essentially as a trans
isomer. Ferulic acid, which is known to effectively cleave free radicals, is known to have a number of
physiological functions, including a strong anti-oxidant, anti-inflammatory and anti-cancer properties,
by increasing the stress response. In this study, the effects of trans ferulic acid, resveratrol and
combinations on time and attenuated rat brain cancer, Glioma (C6) cell line, were investigated in
phenolic compounds with high antioxidant capacity in shells, roots and hips of fruits and vegetables.
Rat brain cancer cell culture (C6); Cells grown with Dulbecco's Minimum Essential Medium
(DMEM), HAMS F 12 (1: 1) + 5% FBS and containing trans ferulic acid, resveratrol and
combinations between doses 0.19-100 M were incubated for 24 hours at 37 C, 5% CO2 incubator.
Cell proliferation was determined by MTT method (3- (4,5-dimethylthiazol-2-yl) -2,5-
diphenyltetrazolium bromide). The IC50 values of the working materials were determined by PROBIT
analysis of SPSS 18 statistical program and applied to C6 cell lines and intracellular metal
determinations were made by DNA-RNA isolations, fluorescence staining and apoptotic cell images
and ICP-MS. cDNAs were obtained from the obtained RNAs and gene expression levels were
examined. The IC50 values of the substances administered in the C6 cells at the 24th hour were
determined as 100 M. The application of trans ferulic acid, resveratrol and combinations led to DNA
damage (Sod: 23.5 fold, 8.8 fold, 4.0 fold, CAT: 5.4 fold, 1.9 fold, 1.3 fold) by inducing increases in
oxidative stress genes in C6 cells. When trans ferulic acid alone was applied, the cytochrome c in the
inner surface of mitochondria with a 2.8-fold increase in BAX gene was cytosolic and complexed with
APAF-1, resulting in a 12.0-fold increase in the amount. In addition, the tumor suppressor is
suppressed by the increase in the p53 gene and apoptosis begins. In resveratrol administration, a 5.8-
fold increase in BAX gene resulted in a 29.9-fold increase in the amount of cytochrome-c and initiated
apoptosis with the apoptosis inducing protein APAF-1. In addition, the combination of trans ferulic
acid and resveratrol has been tumor suppressed by a 16.1-fold increase in p53 gene expression in
glioma cells. Furthermore, in the RAPD-PCR results, the combination of trans ferulic acid and
resveratrol in combination resulted in more deaths than those performed alone, suggesting that this
death may be due to oxidative stress when considered in changes in real-time PCR results. As a result;

Corresponding Author: NEBYE PELN TRKER, pelinturker1988@hotmail.com


1
TRAKYA NVERSTES TEKNOLOJ ARATIRMA VE GELTRME UYGULAMA VE ARATIRMA MERKEZ

1667
Trans ferulic acid, a plant-derived component, and resveratrol brain cancer cell line Glioma C6 cells,
which induce cell death by inducing oxidative stress and also impair mitochondrial structure, thereby
enhancing the function of apoptosis proteins.

KEYWORDS
Trans Ferulic Acid, Resveratrol, Antioxidant, Anticancer, Brain Tumor

1668
Poster Session 13
Submission ID: 1666

THE ANTICANCER AND ANTIOXIDANT EFFECT OF L-DOPA,


GALLIC ACID AND MIXTURES OF THESE SUBSTANCES ON
RATTUS NORVEGICUS BRAIN CANCER CELL LINE (C6)

1 1
AYTEN BOSTANCI , NEBIYE PELIN TRKER

ABSTRACT
The dopamine, a neurotransmitter substance and released from the brain, provides
communication between nerve cells. Dopamine deficiency causes Parkinson's, Alzheimer's and cancer.
It is thought that L-DOPA, an amino acid used as a substitute for dopamine that does not break the
blood-brain barrier, exhibits dopamine metabolism in the brain and thus exhibits an action mechanism.
It is known that L-DOPA, which is used in the absence of dopamine, is found in bean plant.Gallic acid
(3,4,5-trihydroxybenzoic acid, GA), a natural antioxidant, is a polyhydroxyphenol compound with
many fruit types such as green tea, grape, strawberry, and banana.It has been observed that GA
induces apoptosis in cancer cell lines but does not have a cytotoxic effect on healthy cells. In this
study, we aimed to asses single and combined effect of l-dopa and gallic acid on rattus norvegicus
brain cancer (C6) cell lines. C6 cells were grown in cell media (Dulbeccos Minimum Essential
Medium (DMEM), HAMS F 12 (1:1)+ % 5 FBS). Cells, were treated with single and combined in
0.78 to 800 M doses of octopamine, naringin, p-coumaric acid, and then incubated 5% CO2
incubator in 37C for 24 hours. Cell viability were observed by MTT assay ( 3-(4,5-dimethylthiazol-2-
yl)-2,5-diphenyltetrazolium bromide ). IC50 values of the studied substances were determined by
PROBIT analysis of SPSS 18 statistical program and applied to C6 cell lines. DNA-RNA isolations,
intracellular metal determinations by ICP-MS and apoptotic cell images by fluorescence staining were
performed. CDNA libraries were generated from the obtained RNAs. The IC50 values at 24 hours of
the substances administered in C6 cells were determined as M. The application of L-dopa, gallic
acid, and combinations caused oxidative stress in C6 cells, leading to DNA damage by bringing 2,5
fold, 12,2 fold, 3,6 fold, 1.4 fold, 8.7 fold, 4.8 fold increase in SOD gene respectively. Bcl-2, which is
an apoptosis inhibitor, was broadly suppressed in the administration of the combination of L-Dopa and
Gallic acid to glioma cells and showed a 3.0-fold increase in BAX compared with the control.This
increase in BAX gene disrupted the mitochondrial membrane and initiated apoptosis by providing
cytosolic release of cytochrome-c.L-dopa and Gallic acid alone did not inhibit the activity of BCL-2
but increased the mitochondrial ion balance.Furthermore, in RAPD-PCR results, it is thought that
application of the combination of l-DOPA and gallic acid alone is more effective on cancer cells than
antagonistic effect in cells. As a result; The herbal origin of l-DOPA and gallic acid showed an
anticancer effect on the brain cancer cell line glioma C6 cell lines and it has also been determined that
gallic acid induces cell death by producing more oxidative stress in C6 cells compared to l-Dopa and
combination alone.

Corresponding Author: AYTEN BOSTANCI, aytensari@trakya.edu.tr


1
TEKNOLOJ ARATIRMA VE GELTRME UYGULAMA VE ARATIRMA MERKEZ

1669
KEYWORDS
Gallic Acid, L-Dopa, Antioxidant, Anticancer, Brain Tumor

1670
Poster Session 13
Submission ID: 1667

FULVIC ACID POTENTIAL OF HUMUS CREATED IN LOCATIONS


OF THE RHODODENDRON (RHODODENDRON PONTICUM L.) IN
WEST BLACK SEA REGION

1 1 3
ALI GREL , MEHMET ZDEMIR , AHMET TUTAR

ABSTRACT
The aim of this work is to reveal the potential of fulvic acid of humus formed at
Rohododendron (Rhododendron Ponticum L.) locations in West Black Sea Region. It is thought that
humic substances occurring in these locations will constitute an important resource for fulvic acids.
Fulvic acids are macromolecular compounds found application area in wide range as agriculture,
industry, environment and biomedicine. Plot areas was taken from humus created on O2 (organic)
horizon in 27 different locations with 3 replicates in the spreading area of Rhododendron in this work.
It was used 35 ml (25,25 gr) from the resine of XAD-8 for the extract of 100 ml fulvic acid in the
purification of fulvic acid. It was utilized various spectroscopic methods as FTIR and UV for the
characterization of the purified fulvic acid structures. Structural and combination properties related
fulvic acids is obtained using the methods of UV-Visible, IR methods and some decomposition. The
results found from this research were compared with values of 3 different Turkish leonardite.
According to this, the amount of fulvic acids in its content of the humic substances in locations of
Rhododendron in Black Sea region is found as 7,12 % while fulvic acid content of humic substance
originated from leonardite is average 0,2 % . Bu durum ormanlardaki humusun fulvik asit iin nemli
bir potansiyel oluturduunu gstermektedir. This situation, it is seen that humus in the Rhododendron
forests is an important fulvic acid source.

KEYWORDS
Rhododendron humus, fulvic acid, humic substances, leonardite

Corresponding Author: AL GREL, ali.gurel_@hotmail.com


1
ORMAN GENEL MDRL, MARMARA ORMANCILIK ARATIRMA ENSTTS
3
SAKARYA NVERSTES, KMYA BLM

1671
Poster Session 13
Submission ID: 1668

INVESTIGATION OF FELD CROPS AND SOME QUALITY


CHARACTERISTICS (SIDERITIS STRICTA DAVIS ET HUBER-
MORATH) OF CULTIVATED MOUNTAINS

1 1
EMINE BLGNOLU , YKSEL KAN

ABSTRACT
This research has beenconductedunder Konya ecologicalconditionstodetermine the effect on
yeld and qualitysomecharacters of nitrogenfertilizersapplied at the differentdosesof Sideritis stricta in
Medicinal Plants laboratory and Medicinal PlantsExperimental Farm of AgricultureFaculty,
SelcukUniversty. Experiment wasdesigned and applied inrandomizedcompleteplotdesign with
threereplications in the year of 2012-2013. Accordingtoresults of thisresearh the plantheightof Sideritis
stricta 118.33-138.66 cm, the number of floweringbranches 14.30-32.60, freshfloweringyield 513.33-
743.33 kg / da, essential oil yield 0.06-0.10% and major essential oil component ( pinene)
wasvarriedbetween. 27.8 and 38,666. % Accordingto the results of thisresearch; The
highestdrugfloweryield and essential oil yieldformountaintea in Konya and similarecology 10 kg/da
nitrogenfertizerapplication is reasonabled.

KEYWORDS
Mountaintea, Sideritis stricta, Essential oil, pinene, Fertilizer

Corresponding Author: EMNE BLGNOLU, bilginogluemine@gmail.com


1
SELUK NVERSTES

1672
Poster Session 13
Submission ID: 1670

THE ANTIOXIDANT CAPACITY OF THE ACETONE, METHANOL


AND WATER EXTRACTS CRATAEGUS MONOGYNA JACQ SUBSP
MONOGYNA COLLECTED FROM THE EASTERN ANATOLIA
REGION OF TURKEY

1 1 ! 1
ZEL APIK , MERYEM ENGL KSEOLU , RAHIMEH JABERI , GLERAY AAR

ABSTRACT
The genus Crataegus is the largest genus among the subfamily in the family Rosaceae which
comprises 2830 species in 95 genera, which are generally known as the hawthorns. Crataegus
monogyna (C.M) is one of the most common species used as the hawthorn of traditional herbalism.
In traditional medicine, hawthorn has been widely used to treat human diseases. The Crataegus
monogyna has enough research on the biological activities such as antimicrobial, antioxidant,
antitumor, antiviral, cardioprotective, neuroprotective, nephroprotective, hepatoprotective and anti-
inflammatory. The clinical studies have shown that there are no significant adverse effects associated
with hawthorn consumption. There are a great number of studies that suggest the huge potential of
Crataegus monogyna fruit extract in the treatment of various toxicities due to the presence of various
bioactive natural compounds, such as flavanonoids and triterpenic compounds. But up to now there are
limited number of investigations related to Crataegus monogyna Jacq subsp monogyna species
antioxidant activity. In this working was aimed to determine antioxidant activity of acetone, methanol
and water extracts of Crataegus monogyna Jacq subsp monogyna fruits in different concentrations (5;
10; 20; 40 and 80 g/mL) that collected in the eastern Anatolia, Turkey by DPPH(2, 2-Diphenyl-1-
picrylhydrazyl) radical scavenging method. The fruit extracts are used as fresh due to their broad
pharmacological effects and minimal adverse effects. Extracts at different concentrations were added
to 0.5 mL of methanolic DPPH solution (0.1 mmol). The estimated time of reaction (30 min) was
determined by considering the reduction of the absorbance at 517 nm. The absorbance was measured
at room temperature, in darkness, against a blank. The absorbance of the control (3ml of methanol in
0.5 mL of DPPH solution) was measured. According to the results of the study, Crataegus monogyna
Jacq subsp monogyna has antioxidant activity. The strongest antioxidant activity of acetone and
methanol extract could be due to the presence of flavonoids and phenols. The 40-80 g/mL doses of
fruit extracts were more effective than other doses. As a result, Crataegus monogyna Jacq subsp
monogyna can be recommended for future clinical trials and chemotherapheutic drugs aimed at
examining its beneficial effects.

KEYWORDS
Antioxidant capacity, DPPH, Crataegus monogyna

Corresponding Author: ZEL APIK, ozlcpk.36@gmail.com


1
NVERSTE

1673
Poster Session 13
Submission ID: 1671

USE OF AROMATIC PLANTS TO REDUCE RUMINAL


METHANOGENESIS AND AMONIA CONCENTRATION

1 2 3 3
SEMA YAMAN , SIBEL CANOULLARI DOAN , MUSTAFA BOA , LKNUR UAK

D
ABSTRACT
Aromatic plants, also known as herbs and spices, have been used in the Middle East since

TE
approximately 5000 BC for their preservative and medicinal properties, in addition to enhancing the
aroma and flavour of foods. Currently, there is an increasing interest in using herbs and spices in
animal nutrition, in order to replace the use of antibiotics and ionophore anticoccidials, after the ban of
antibiotics used as feed additives in 2006 by EU. The aromatic plants, their extracts and their essential
oils have been examined due to their advantages over the antibiotics as growth promoters. They are
EN
residue free and generally recognized as safe. Aromatic plants and its essential oils have been used to
manipulate ruminal metabolism in order to improve feed efficiency and animal productivity. Some
promising results have been obtained from in vitro batch culture studies that essential oils or their
components have the potential to improve nitrogen and/or energy utilization in ruminants. It has
ES
shown that some aromatic plants derived substances had a strong bactericidal activity against
pathogenic bacteria. This has prompted some researchers to study potential of aromatic plants
derivatives to reduce ruminal methanogenesis for limiting the release of this gas into the atmosphere.
In vivo research using novel aromatic plants in rumen nutrition has the potential to benefit animal
production and health, as well as the environment. In this review effect of aromatic plants and its
PR

essential oils on reducing ruminal methane and ammonia and its mode of action in the rumen will be
focused.

KEYWORDS
T

aromatic plant, essential oils, ruminant, methane, ammonia, mitigation


O
N

Corresponding Author: SEMA YAMAN, semayaman@ohu.edu.tr


1
MER HALSDEMR NVERSTES, TARIM BLMLER VE TEKNOLOJLER FAKLTES, HAYVANSAL
RETM VE TEKNOLOJLER BLM, 51240 NDE
2
MER HALSDEMR NVERSTES, TEKNOLOJLER FAKLTES, HAYVANSAL RETM VE
TEKNOLOJLER BLM, 51240 NDE
3
MER HALSDEMR NVERSTES, BOR MESLEK YKSEKOKULU, BOR, NDE

1674
Poster Session 13
Submission ID: 1672

USE OF LICORICE ROOT (GLYCYRRHIZA GLABRA) IN ANIMAL


FEED AS ACTIVE INGREDIENT

1 ! ! 1
SIBEL CANOULLARI DOAN , SEMA YAMAN , MUSTAFA BOA , LKNUR UAK

D
ABSTRACT
Interest in aromatic plants and extracts has been increased following the ban of antibiotics use

TE
in animal nutrition in 2006 due to the risk of animal and human health. These plants and extracts are
natural and reliable substances and they have been used widely for medical purposes and as spices for
centuries. Since food safety is at the forefront nowadays, the use of natural additives and extracts have
gained of importance. For this reason, great emphasis is placed on the use of plants and extracts
containing antioxidant active compounds in their structure. As a matter of fact, these plants and
EN
extracts have some characteristics such as antimicrobial, antioxidant, antilipidemic, antifungal,
antiviral, digestive system stimulator and also have some positive effects on organism such as
optimizing performance, improving feed efficiency and vitality. Licorice root (Glycyrrhiza glabra) is
among the oldest and most widely known medical plants in the world. A large number of
ES
pharmacologically active compounds have been isolated from the Licorice root plant. The main
components of these bioactive compounds are triterpenoid saponins and various types of phenolic
compounds. Experiments on animals have shown that the bioactive substances contained in the plant
caused significant decreases in plasma total lipid, cholesterol, triglyceride, LDL, VLDL and increases
in the HDL-cholesterol content. It has been shown that licorice root causes an increase in antioxidant
PR

enzyme activity. This review provides a brief overview on general structure of the Licorice root and its
use as an active ingredient in animal nutrition.

KEYWORDS
T

active ingredient, animal nutrition, aromatic plant, extracts, Licorice root


O
N

Corresponding Author: SBEL CANOULLARI DOAN, sibelcanogullari@gmail.com


1
MER HALSDEMR NVERSTES

1675
Poster Session 13
Submission ID: 1673

MEDICINAL USES AND CHEMICAL CONTENTS OF MARIGOLD


(CALENDULA OFFICINALIS) PLANT

1 1
CAHIDE YETI , AHANE FUNDA ARSLANOLU

ABSTRACT
It is as old as human history that people meet their clothing, food and treatment needs from
natural products. The increase in the world population and the development of the industry have
rapidly increased the availability of the active substances from the plants found in the ending country,
and the demands of the countries of the world have also increased. When the origins of the drugs
developed between 1981-2006 were examined, 32% of them were proved to be natural or
semisynthetic. In addition, the fact that nearly half of the drugs are based on natural resources,
prompts drug development efforts to the nature. The effect of herbal medicines on human health is
extremely important. Herbal remedies for the prevention and treatment of various diseases have been
used since ancient times. Marigold (Calendula officinalis) used for the treatment of external and
internal wounds is not cultured for any purpose except being an ornamental plant in our country. . For
this reason, cultural studies and evaluation as a herbal drug in our country at the scientific level of
work is almost not to be tried. The plant, which is unique to the Mediterranean, is a single annual and
perennial varieties and has 20-30 varieties. The plant is green every season. It is called as pot
marigold, calendula, ringerblume and souci des jardins in various countries of the world,. It is
known in our country with the names as aynsafa, kadife iei and tbbi nergis. It is also used
medically in addition to giving color to desserts. Chemical ingredients include triterpenes, flavonoids,
essential oils and sesquiterpenes. As a detox in chronic skin disorders such as eczema, acne, in bowel
and liver diseases, bed wounds, antiseptic, hepatitis (a, b, c), in the treatment of foot wounds, baby
creams, wound healing, diuretic, diarrhea, antibacterial and antifungal effect is used as a medicinal
plant for the purpose. In this study, the chemical content of Calendula officinalis plant and its use as
medicinal plant will be investigated.

KEYWORDS
Calendula officinalis, Aynsefa, marigold, medicinal plant, herbal drug

Corresponding Author: CAHDE YET, chd.yts@gmail.com


1
ONDOKUZ MAYIS NVERSTES ZRAAT FAKLTES TARLA BTKLER BLM

1676
Poster Session 13
Submission ID: 1675

NATURAL DYES FROM PLANTS

1 1
MERVE YZBAIOLU , AYE KURUZM-UZ

ABSTRACT
NATURAL DYES FROM PLANTS Merve Yzbaolu, Aye Kuruzm-Uz Hacettepe
University Faculty of Pharmacy, Dept. of Pharmacognosy, 06100, Ankara, TURKEY Plants in
modern society are valued for their flavor, fragrance, medicinal and healthful qualities, economic and
industrial uses, pesticidal properties and colorizing materials (dyes). Natural dyes are derived from
naturally occurring source such as plants, insects, animals, clays and minerals. The range of colors is
limited, but lovely. Due to their non-toxic properties, less side effects, more medicinal values and eco-
friendliness, natural dyes are suitable for dyeing textiles, drugs, foods, cosmetics etc. They can also
provide extra properties such as UV protection, skin moisturizing, anti-aging, antimicrobial, insect
repellent and deodorizing (1). Most natural dye colors are found in the roots, bark, leaves, flowers,
fruits, skins and shells of plants. The well-known ancient dyes include red dye from roots of Rubia
tinctorum L. and leaves of the plant Lawsonia inermis, blue indigo dye from leaves of Indigofera
tinctoria, yellow dyes from stigmas of saffron (Crocus sativus ) and rhizome of turmeric (Curcuma
longa). Flavonoids (flavonols, flavones, flavanones, chalcones/ aurones, anthocyanins),
hydroxycinnamic acids, carotenoids, tannins, naphtoquinones and anthraquinones are the phyto-colour
compounds found in the plants. As synthetic dyes develop, they cause several kinds of pollution in the
environment. Likewise some dyes can cause allergic dermatoses, respiratory diseases, contact
dermatitis and mutagenicity. Synthetic dyes are so problematic because the families of chemical
compounds that make good dyes are also highly toxic and carcinogenic to humans. In recent times
natural dyes has regained importance due to the environmental awareness about health problems
caused by synthetic dyes. But contrary to this popular opinion, natural dyes are often neither safer nor
more harmful than synthetic dyes. They are less permanent, more difficult to apply, wash out more
easily, and often involve the use of highly toxic mordants. Mordants help make the dye colour fast by
chemically bonding the dye to the fabric. Not all natural dyes require toxic mordants to create light
and wash fast colorways. Also, garments dyed with lichens, onion skins etc. don't always have a
mordant, which could be good for people with sensitivities because they are substantive dyes- that is,
they don't require the use of a mordant. But, some natural dyes, such as the hematein derived from
logwood (Haematoxylonam pechiamum) are themselves significantly poisonous. It is very important
that to improve the quality and quantity of dye yielding plants and to detect their toxicological
characters are required. References: Ashis KS, Konar A. 2011. Dyeing of Textiles with Natural Dyes,
Natural Dyes, Kumbasar EA (Ed.), InTech, 29- 56. "Dyes and Chemical Sensitivities."
Organic_Clothing. Accessed March 31, 2017.
http://organicclothing.blogs.com/my_weblog/2005/10/dyes_and_chemic.htm Kasturi A. 2015.
Hazardous Materials In Chemical Dyes And Natural Solutons, International Journal Of Engineering
Research (Online) S-1. Senthilkumar RP, Bhuvaneshwari V, Sathiyavimal S, Amsaveni R, Kalaiselvi

Corresponding Author: MERVE YZBAIOLU, myuzbasioglu13@gmail.com


1
HACETTEPE NVERSTES ECZACILIK FAKLTES FARMAKOGNOZ ABD

1677
M, Malayaman V. 2015. Natural Colours from Dyeing Plants for Textiles, International Journal of
Biosciences and Nanosciences 2 (7), 160-174.

KEYWORDS
Natural dyes, mordants, synthetic dyes, anthraquinones, naphthoquinones

1678
Poster Session 13
Submission ID: 1676

THE SUSTAINABILITY, PRODUCTION AND QUALITY FROM SOME


OF BLACK CUMIN SPECIES IN TURKEY

1 2 1
IREM AYRAN , HAKAN VATANSEV , YKSEL KAN

ABSTRACT
Black cumin (Nigella sativa L. and Nigella damascena L.) is a annual, herbaceous that is plant
belongs to Ranunculacea family. The origin of black cumin extend to from Eastern Mediterranean
countries to Eastern and Southern Europe. Nigella sativa L. is widespread cultivated in Afyon, Burdur,
Isparta, Ktahya and Konya regions in Turkey. Nigella damascena L. species is not yet cultivated in
our country. The parts of the black cumin used are seeds and have a very important place due to fixed
oil, volatile oil and nutrients constant obtained from its seeds. The oil obtained from the seeds of the
plant is used in the treatment of many diseases with medical purposes as well as being used as a spice
and flavor in food products. Nigella sativa L. and Nigella damascena L. are commercially available
varieties of plants in Turkey. Known the name of black cumin species commonly used in Turkey is
Nigella sativa L. whereas the use of Nigella damascena is not common. In this study, information
about the biology, chemical structure, production and evaluation of every both species will be given.

KEYWORDS
Black cumin, fixed oil, essential oil, traditional medicine

Corresponding Author: REM AYRAN, irem.ayran@gmail.com


1
SELUK NVERSTES
2
NECMETTN ERBAKAN NVERSTES

1679
Poster Session 13
Submission ID: 1678

EFFECTS OF GREEN TEA IN THE TESTICULAR TISSUE

1 2
MUSA TATAR , TUBA TATAR

ABSTRACT
Tea is commonly consumed beverage in the world. Tea can be categorized into three main
types, depending on the level of oxidation, as green tea, oolong tea and black tea. Tea polyphenols are
important due to the positive association between tea consumption and beneficial health effects. Green
tea extract contains polyphenols which make up 2535% of the dry weight of green tea leaves. Green
tea polyphenols including epigallocatechin-gallate (EGCG), epigallocatechin (EGC), epicatechin-
gallate (ECG), and epicatechin(EC) show antioxidant properties. Many investigations have been
performed on the antioxidant activity of tea catechins on testicular tissue stimulated with agent which
causes oxidative stress. Messarah M. et al. report that green tea has protective effect on toxicity of
testis tissue via arsenic. In a study, green tea extracts show ameliorative effects on nicotine-induced
oxidative stress as well as the reproductive effects by improving the oxidative status, semen quality
and the testicular histological damage. In another study, it is observed similar results, and also an
increase number of Leydig cells in nicotine stimulated rats. Awoniyi DO. et al. report that green tea
supplementation causes an increase in GSH levels, in the superoxide dismutase activity, and a
decrease in lipid peroxidation in testicular tissue of rats stimulated with oxidative stress agent.
Oxidative stress increases in diabetes due to overproduction of reactive oxygen species (ROS) and
decreased efficiency of antioxidant defenses. Therefore diabetes mellitus causes harmful effects on
male sexual and reproductive functions. It is observed that administration of green tea to diabetic rats
improves the testis structure, induces the proliferation index and reduces apoptotic index. Green tea
affects in the protection of testicular tissue against oxidative damage by possibly increasing the
antioxidant defense mechanisms in rats. Excess amounts of ROS have adverse effects on sperm
motility and fertility due to damaging to lipids and DNA of spermatozoa. Administration of green tea
supplements to rats which stimulated with oxidative stress causes increasing the antioxidant defence
mechanisms and thereby improving the sperm quality and function. In conclusion, antioxidant
property of polyphenols in green tea has beneficial effects on testicular tissue damages due to
oxidative stress.

KEYWORDS
Green Tea, Testicular Tissue, Polyphenols

Corresponding Author: MUSA TATAR, musatatar88@hotmail.com


1
MEHMET AKF ERSOY NVERSTES
2
GAZ NVERSTES

1680
Poster Session 13
Submission ID: 1679

SPICES IN POULTRY NUTRITION: ANTIOXIDANT EFFECT AND


MODES OF ACTION

1 2 3 3
ZEYNEP AHAN , FATMA YENILMEZ , UUR SERBESTER , LADINE ELIK

D
ABSTRACT
In recent years, spices and their components have received attention as antioxidant source.

TE
Some spices exert antioxidant properties, and may enhance immune status. The mode of action of
spices on poultry contain high concentrations of phenolic compounds that have strong H-donating
activity. The aim of this paper is to provide an overview of the published data on the potential of
spices and their components in poultry nutrition, and to describe their possible modes of action. The
current knowledge on potential antagonistic and synergistic effects is presented and areas for future
research are proposed. EN
KEYWORDS
ES
Spices, poultry nutrition, antioxidant effects, mode of action
PR
T
O
N

Corresponding Author: LADNE ELK, ladine@cu.edu.tr


1
ADIYAMAN NVERSTES, KAHTA MESLEK YKSEKOKULU, ADIYAMAN
2
UKUROVA NVERSTES, TUFANBEYL YKSEKOKULU, ADANA
3
UKUROVA NVERSTES, ZRAAT FAKLTES, ZOOTEKN BLM, ADANA

1681
Poster Session 13
Submission ID: 1680

EXTRACTION METHODS OF ESSENTIAL OILS FROM MEDICINAL


AND AROMATIC PLANTS

1 1
HATICE BETL KAPLAN , SEBAHATTIN NAS

ABSTRACT
Essential oils are used in a wide variety of consumer goods such as detergents, soaps, toilet
products, cosmetics, pharmaceuticals, perfumes, confectionery food products, soft drinks, distilled
alcoholic beverages (hard drinks) and insecticides. The world production and consumption of essential
oils and perfumes are increased very fast. And also the use of alternative/herbal medicine for the
prevention and treatment of various illnesses has been increasing interest worldwide in recent years. A
wide range of technologies is available for the extraction of active components and essential oils from
medicinal and aromatic plants. Production technology is an essential element to improve the overall
yield and quality of essential oil. The traditional technologies pertaining to essential oil processing are
of great significance and are still being used in many parts of the globe. The extraction methods are
traditional technics such as distillation, expression (cold pressing), solvent extraction and modern
(non-traditional) technics such as Supercritical fluid extraction, Microwave-assisted extraction, Solid
phase micro-extraction, Simultaneous distillation extraction. All methods has advantages and
disadvantages. The choice of extraction method depends on the economic feasibility and suitability of
the process to the particular situation. The various extraction methods of essential oils from medicinal
and aromatic plants oils are reviewed in this prensentation.

KEYWORDS
Medicinal Plants, Aromatic plants, extraction methods, essential oil

Corresponding Author: HATCE BETL KAPLAN, hbetulk@pau.edu.tr


1
PAMUKKALE UNVERSTY, ENGNEERNG FACULTY, FOOD ENGNEERNG DEPARTMENT

1682
Poster Session 13
Submission ID: 1681

COMPARISON OF ANTIBACTERIAL ACTIVITIES OF GRAPE SEED,


BLUEBERRY POMACE AND FOX GRAPE POMACE EXTRACTS ON
GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

1 ! 1
MELIKE DEMRKOL , MER FARUK ELK , ZEKAI TARAKI

ABSTRACT
Many in vivo and in vitro studies suggest that flavonoids possess a variety of biological
functions including antiallergic, anticarcinogenic, antihypertensive and antimicrobial properties. They
are potential antioxidants thus may play an important role in the persistence of health. There are many
plant based compounds exhibiting antimicrobial activity against pathogen microorganisms due to the
presence of hydroxyl groups and conjugated double bonds in the reactive groups such as flavonoids.
Berries and grapes are regarded as rich fruits in terms of their flavonoid contents. In this study, the
antibacterial activities of extracts of dried grape (Vitis vinifera) seed, fox grape (Vitis labrusca) and
blueberry (Vaccinium myrtillus) pomaces will be investigated. Grape seeds were sun-dried while both
pomaces were liyophilized. After grinding, the extracts of regarding powders will be prepared using
methanol as the solvent. All extracts will be applied at different concentrations in order to observe and
evaluate their antibacterial activity. Escherichia coli and Staphylococcus aureus will be used as model
microorganisms representing gram negative and gram positive bacteria, respectively. Antibacterial
activities of extracts will be determined using agar disc diffusion method. Bacteria will be cultivate
from stock and after a couple of passages they will be grown until mid-log phase in Tryptic Soy Broth
(TSB). Following their plating on Mueller-Hinton agar, discs that are impregnated with different
concentrations of phenolic extracts will be placed on the agar. After incubation, the inhibition zones
around the discs will be measured milimetrically and the results will be evaluated.

KEYWORDS
Antimicrobial, antibacterial, berry, grape, drying, extract, phenolics

Corresponding Author: MER FARUK ELK, ofarukcelik@gmail.com


1
ORDU NVERSTES

1683
Poster Session 13
Submission ID: 1682

THE CHARACTERISTICS AND USAGE OF MARIGOLD


(CALENDULA OFFICINALIS L.) FROM PAST TO FUTURE

1 1
IREM AYRAN , YKSEL KAN

ABSTRACT
Marigold (Calendula officinalis L.) is a annual, herbaceous that is plant belongs to Asteraceae
family. It was recorded that the origin of Marigold is a plant originating in Central and Southern
Europe, Western Asia and America. It has been almost every flora of Turkey. At the same time , This
plant cultivated in Turkey is one of medicinal and aromatic plants. Especially in term of medicinal,
The plant contains seconder metabolite actived such as essential triterpene saponins, flavonoids,
carotenoids and essential oils. In addition to its use as food, cosmetics, and paint, flowers of the same
plant are also used in the treatment of many diseases. At the same time, the plant is widely used as a
medicinal oil in traditional medicine applications in Turkey. It has been determined that studies on
marigold have antiinflammatory, antimicrobial, antiviral, antihypotensive and antilipid properties. In
this study, information about the biology, chemical structure, production and evaluation of the plant
will be given.

KEYWORDS
Marigold, medicinal oil, triterpen saponine, essential oil, traditional medicine

Corresponding Author: REM AYRAN, irem.ayran@gmail.com


1
SELUK NVERSTES

1684
Poster Session 13
Submission ID: 1683

CROSS-GENERA TRANSFERABILITY OF CHLOROPLAST


MICROSATELLITE MARKERS IN THE GENERA OF LAMIACEAE

1 1 1
EMINE UYGUR GER , MEHMET KARACA , AYE GL NCE

ABSTRACT
Microsatellites, also referred to as simple sequence repeats (SSRs) or simple tandem repeats
(STRs), were first described by Litt and Lutty in 1989. Microsatellites consist of tandemly repeated
mono-, di-, tri-, tetra- or penta- and hexa-nucleotide motif units distributed throughout the genomes of
most eukaryotic organisms. The differences in the tandemly repeating motifs are caused by DNA
polymerase slippage during replication, or slipped strand mispairing. Repeat differences in
microsatellite motifs are powerful DNA markers because they are highly polymorphic even between
closely related lines and varieties, reproducible and co-dominant markers exhibiting multiallelic nature
and inherited as simple Mendelian fashion. Microsatellites can be found in nuclear and organellar
genomes, which are enclosed in chloroplast and mitochondria. These tandemly repeated DNA can be
located within transcribed, un-translated and un-transcribed regions of the genomes including the
regulatory regions. Microsatellites found on the transcribed and untranslated regions of genes seem to
have higher level of cross-transferability within the genetically related organisms. Microsatellites
located on the chloroplast genome of higher plants are usually composed of mononucleotide (usually
As and Ts) repeats and occasionally di-nucleotide repeats. The utilization of chloroplast microsatellites
has been reported by Taberlet et al. in 1991. Later on, several universal primers were reported for
amplifying chloroplast regions in various crops. In the present study a total of 10 conserved
chloroplast microsatellite primer pairs developed from chloroplast DNA sequences of Nicotiana
tabacum were used to amplify chloroplast microsatellites of Sideritis stricta, Stachys spp., Salvia
pisidica and Thymus cilicicus, members of the family Lamiaceae. Two different touch-down
polymerase chain reaction profiles differing in the annealing temperatures were used in the
amplification studies before high resolution of agarose gel electrophoresis separation. Amongst the 10
conserved chloroplast primer pairs (CCMP), CCMP09 did not amplify DNA templates of the genera
while CCMP05 only amplified template of Thymus cilicicus. Rest of the CCMP primer pairs could
amplify DNA templates of the genera tested. The range of amplified product varied from 80 bp to
about 200 bp. Among the CCMP amplicons, CCMP01 markers contained TA dinucleotide repeats
while rest of the CCMP markers consisted of A or T mononucleotide microsatellites. Successful
amplification of primer pairs obtained from chloroplast of Nicotiana tabacum indicated that
chloroplast genome sequences are highly conserved due to the low level of mutation rate among the
plant species. Because chloroplast genomes are haploid and contain many copies in a cell of higher
organisms and most chloroplast genomes have maternal inheritance, cross-genera transferable
chloroplast microsatellite markers reported for Sideritis stricta, Stachys spp., Salvia pisidica and
Thymus cilicicus could be successfully used in genetic, conservation and species identification studies.
Maternal inheritance of these markers could be used in determination of hybrid identification of the
genera tested.

Corresponding Author: EMNE UYGUR GER, emineuygur@akdeniz.edu.tr


1
AKDENZ NVERSTES

1685
KEYWORDS
Chloroplast genome, microsatellites, SSR, Sideritis, Stachys, Salvia, Thymus

1686
Poster Session 13
Submission ID: 1685

APPLICATION OF SINGLE PRIMER AMPLIFICATION REACTION


(SPAR) MARKERS IN FINGERPRINTING STUDIES OF AROMATIC
PLANTS

1 1 1
EMINE UYGUR GER , MEHMET KARACA , AYE GL NCE

ABSTRACT
The theoretical advantages of using genetic markers, the potential value of genetic marker
linkage maps and direct selection in plant breeding were first reported about eighty years ago.
However, it was not until the advent of DNA marker technology in the 1980s, that a large enough
number of environmentally insensitive genetic markers generated to adequately follow the inheritance
of important agronomic traits and since then DNA marker technology has dramatically enhanced the
efficiency of plant breeding. The 1980s could be considered as the birth of molecular breeding. The
discovery of polymerase chain reaction (PCR) has facilitated the development of new marker systems
that have a variety of applications, some of which include: (i) fingerprinting of genotypes or varieties
for identification and bar-coding purposes; (ii) mapping of genes and quantitative trait loci for
important traits; (iii) positional cloning of desired genes; (iv) identification of chromosome segments
carrying desired loci; (v) establishing phylogenetic relationships among different genotypes or species;
(vi) selection of suitable parents for hybrid breeding and gender identification; (vii) assessing the basis
of somaclonal variation and epigenetic modifications; (viii) identification of pathogen races and
biotypes; (ix) prediction of heterotic cross combinations; (x) gene pyramiding; and (xi) management,
utilization and preservation of genetic resources. The various PCR-based marker systems are of two
types depending on the primers used for in vitro DNA amplification: (i) arbitrary or semi-arbitrary
primed PCR marker systems which are developed without prior genomic sequence information of the
plant species under the study (AP-PCR, DAF, RAPD, AFLP, ISSR, DAMD etc.,), and (ii) site-
targeted PCR marker systems which are developed from known genomic DNA sequences of the plant
species under the study (CAPS, SSR, SCAR, SRAP). Single primer amplification reaction (SPAR)
marker system belongs to the first group; being in the semi-arbitrary primed PCR marker systems. In
the present study, we investigated whether SRAP markers are useful in fingerprinting studies of the
genera Thymus L., Origanum L., Rosmarinus L. and Ocimum L. represented with Thymus serpyllum,
Origanum majorona, Rosmarinus officinalis and Ocimum basillicum. High genomic DNA samples of
above mentioned plant species were extracted. About 60-120 ng genomic DNA templates were
amplified by touchdown polymerase chain reactions (PCRs) using a single primer. Amplified products
were separated by agarose gel electrophoresis and SPAR markers of the plant samples were visualized
with the help of ethidium bromide staining. Analysis revealed that all the six primers could
successfully amplify the genomic DNA samples of the aromatic plant species used in the study. In
each SPAR marker profiles among the six single primers there were densely amplified products
ranging one to three along with some other less dense products. The SPAR marker are produced using
a single primer thus these densely amplified products contained primer binding sites at both terminal

Corresponding Author: EMNE UYGUR GER, emineuygur@akdeniz.edu.tr


1
AKDENZ NVERSTES

1687
ends while these less dense amplicons contained one primer binding sites at one terminal ends. Present
study revealed that SPAR markers could be successfully used in genetic, conservation and species
identification studies of genotypes and varieties in the genera Thymus, Origanum, Rosmarinus and
Ocimum.

KEYWORDS
DNA markers, PCR-based markers, Thymus serpyllum, Origanum majorona, Rosmarinus
officinalis, Ocimum basillicum

1688
Poster Session 13
Submission ID: 1687

INVESTIGATION OF CHEMICAL CONTENT OF EREMURUS


SPECTABILIS BIEB. EXTRACT USING SUBCRITICAL WATER
EXTRACTION

1 1 1 2 2
BRAHIM TEGIN , BETL SADIK , MEHMET FDAN , ERDAL YABALAK , A. MURAT GIZIR

ABSTRACT
Nowadays, food and pharmaceutical industry pay great attention to Subcritical water
extraction method (SWE) attributed to being powerful technique for the extraction of various valuable
solid samples [1, 2]. In addition, it has been used in environmental applications to extract hazardous
components from various matrixes [3]. Subcritical water is known as water in the temperature range of
100-
altering the temperature. Thus, SWE can be used as a non-polar solvent at high temperature [5-8].
Subcritical water extraction is a distingue method when comparing traditional methods due to being
time-saving, rapid, green, and selective method [3, 9]. Thus, subcritical water extraction method was
performed to determine chemical components of Eremurus Spectabilis Bieb. in this study. A large
number of plants have been discovered by mankind since centuries. People use these plants for
different purposes. This plant is used in medicinal and glue purposes in food grown in the region [7].
Samples of Eremurus Spectabilis Bieb were dried and crushed for extraction using the experimental
set-
and 40 bar. Samples were analyzed by GC-MS according to Wiley7Nist05.L, NIST05a.L, and
W9N11.L, to determine extracted compounds. Some of these compounds are as follows: Glycidyl
alcohol, Acetic acid, 1,2-Propanediol, 3-Furanmethanol, 1,2,3-Propanetriol, Dimethyl phthalate, D-
Erythro-Pentose, Hexadecanoic acid, 9-Octadecenoic acid.

KEYWORDS
Eremurus Spectabilis Bieb, Subcritical water extraction

Corresponding Author: BRAHM TEGIN, ibrahim.tegin@gmail.com


1
SRT UNVERSTY
2
MERSN UNVERSTY

1689
Poster Session 13
Submission ID: 1688

RESOLUTIONS OF MINISATELLITE (DAMD-PCR) AND


MICROSATELLITE (SSR) MARKERS IN SALVIA L.

1 1 1
MEHMET KARACA , AYE GL NCE , ADNAN AYDIN

ABSTRACT
Sage (Salvia L.) is one of the largest genera in the family Lamiaceae. The genus consisted of
about 1000 species and many of which are used in spices, drug and fragrance industries. DNA markers
have opened a new era in plant breeding, conservation, management and utilization. However,
application of DNA markers in many Salvia species lags behind many other plant species including
the family Lamiaceae. DNA markers are very useful in those studies involving in the determination of
genetic mapping, gene identification, parentage selection, identification of cultivars, marker assisted
breeding and selection. Single nucleotide polymorphisms (SNPs) and microsatellites (SSRs) are the
two most widely used and most powerful molecular markers. However, utilization of these markers in
Salvia L. has not reached the optimal level. This study was undertaken to compare resolutions power
of minisatellites in terms of direct amplification of minisatellite DNA (DAMD-PCR) and
microsatellite in terms of simple sequence repeats (SSRs) within and between some Salvia species
naturally occurring in Antalya. A minisatellite is a tract of tandemly repetitive DNA motifs repeated
many times, ranging in length from 1060 base pairs. They are usually 0.220 kb long and are usually
prominent in the heterochromatin regions being the most occurring in the centromeres and telomeres
of chromosomes. Minisatellite markers or minisatellite enriched markers can be detected using direct
amplification of minisatellite DNA (DAMD-PCR). Amplification of DAMD-PCR markers is obtained
when flanking regions of minisatellites have inverted DNA sequences to which minisatellite core
primers bind during PCRs. Although they have high level of polymorphism and do not require
sequence information for development, DAMD-PCR markers have lower level of reproducibility in
comparison to microsatellite markers which are also known as simple sequence repeats (SSRs).
Microsatellite is the DNA marker of choice and dominated plant molecular research during the last
decade of the past century and the first decade of the present century. Microsatellites are tandem
repeating mono-, di-, tri-, tetra- or penta- and hexa-nucleotide motif units distributed throughout the
genomes of every eukaryotic organism sequenced so far. Microsatellites are highly polymorphic and
usually co-dominant DNA markers. Development of microsatellite markers requires low amount of
DNA, and can be easily automated for high throughput screening, transferable between laboratories.
Polymorphisms in microsatellites and minisatellites of genetically related organisms are due to
recombination (i.e. unequal crossing-over) and replication (DNA polymerase slippage) errors. In the
present study 3 Salvia species (European sage, meadow sage and balsamic sage) and 12 genotypes
four of which representing each species were studied. DNA markers obtained using 8 SSR primer
pairs and 8 DAMD-PCR primers were separated in 3% (for SSRs) and 2% (for DAMD-PCR) high
resolution agarose gel electrophoresis after touch-down polymerase chain reactions. Results clearly
indicated that the level of polymorphisms were greater in SSR markers than that of the DAMD-PCR
markers. The DAMD-PCR provided a moderate level of polymorphism between the species studied.

Corresponding Author: MEHMET KARACA, mkaraca@akdeniz.edu.tr


1
AKDENZ NVERSTES

1690
However, the levels of polymorphic DAMD-PCR markers were low within the species. The levels of
transferability of DAMD-PCR markers between the species were higher than SSR markers. In
conclusion the present study indicated SSR markers provide greater resolution for intra-species while
the DAMD-PCR markers are more suitable for inter-species genetic studies.

KEYWORDS
DAMD-PCR, Microsatellites, Minisatellites, Simple sequence Repeats, Polymorphisms

1691
Poster Session 13
Submission ID: 1690

THE DETERMINATION OF MINERAL AND HEAVY METAL


CONTENTS OF ECHNACEA SPECIES CULTIVATED IN TURKEY

1 1
SADYE AYE ELK , YKSEL KAN

ABSTRACT
This study was carried out in five different echinacea species in the Medicinal and Aromatic
Plants Application Farm of the Faculty of Agriculture of Selcuk University in 2013 and 2014 in the
ecological conditions of Konya. B, Ca, K, Na, P and S minerals and Al, Cd, Co, Cr, Cu, Fe, Mn, Pb,
Mo, Ni and Zn heavy metals were investigated in the herb and root samples taken during the full
flowering period of five different echinacea species (Echinacea purpurea var. purpurea, Echinacea
pallida var. pallida, Echinacea paradoxa var. paradoxa, Echinacea purpurea var. baby white swan ve
Echinacea purpurea var. double decker species). NMKL 161 method and ICP-AES instrument were
used for determining mineral substances. The highest B, Ca, K, Na, P and S contents of the herbs of
Echinacea species were respectively, Echinacea purpurea var. double decker 135,029 ppm, Echinacea
purpurea var. double decker 31185,018 ppm, Echinacea purpurea var. baby white swan 21968,858
ppm, Echinacea pallida var. pallida 104,742 ppm, Echinacea purpurea var. purpurea 2490,672 ppm
and Echinacea paradoxa var. paradoxa 1754,594 ppm. The highest Al, Cd, Co, Cr, Cu, Fe, Mn, Pb,
Mo, Ni and Zn contents of the herbs of Echinacea species were respectively, Echinacea purpurea var.
baby white swan 432.195 ppm, Echinacea purpurea var. purpurea 0.111 ppm, Echinacea paradoxa var.
paradoxa 0,395 ppm, Echinacea paradoxa var. paradoxa 2,615 ppm, Echinacea purpurea var. purpurea
22,535 ppm, Echinacea purpurea var. baby white swan 451.350 ppm, Echinacea paradoxa var.
paradoxa 46.217 ppm, Echinacea paradoxa var. paradoxa 21.366 ppm, Echinacea purpurea var.
purpurea 3.364 ppm, Echinacea paradoxa var. paradoxa 2.936 ppm ve Echinacea paradoxa var.
paradoxa 22.218 ppm. The highest B, Ca, K, Na, P and S contents of the roots of Echinacea species
were respectively, Echinacea purpurea var. double decker 102,620 ppm, Echinacea purpurea var.
purpurea 38805,7 ppm, Echinacea paradoxa var. paradoxa 20656,313 ppm, Echinacea pallida var.
pallida 2062,226 ppm, Echinacea paradoxa var. paradoxa 2262,165 ppm and Echinacea paradoxa var.
paradoxa 2181,838 ppm.

KEYWORDS
Echinacea, minerals, heavy metals, herb, root.

Corresponding Author: SADYE AYE ELK, sacelik@selcuk.edu.tr


1
SELUK NVERSTES

1692
Poster Session 13
Submission ID: 1691

THE DETERMINATION OF MEDICAL PLANTS USED IN THE


TREATMENT OF DISEASES IN ERZURUM

1 1 2 3 1
SERAP BUZTEPE , AKAR KARAKO , EYYUP TELCI , AYHAN BUZTEPE , FATMA GR

ABSTRACT
In daily life, medicinal and aromatic plants are used as a traditional method for the protection
and improvement of health, and for the treatment of diseases in Turkey. It is known by people living in
the city of Erzurum located in Eastern Anatolia region of Turkey that, in here, the use of plants for the
health field is widespread. The aim of our study is to determine which medicinal plants are used for
which illness by the people living in Erzurum city. The research was carried out on 73 volunteers,
were selected randomly, living in Erzurum. The data were collected through a questionnaire, also
including open-ended questions, prepared by the researchers. Descriptive statistics, chi-square and
Fisher's exact tests were used for the analysis of data. The mean age of the participants was 41.34
15.23 (mean standard deviation). 46.6% of the participants were women and 53.4% were men; 30%
are high school graduates and 42.5% are university graduates. It was seen that participants had
obtained knowledges about the use of plants from family (28.8%), friends (20.5%), relatives (17.8%)
and health workers (15.1%). 51 plants used by the participants for the treatment of diseases were
identified in this study. The most commonly used plants were linden (Tilia Cordata) (19.2%), ginger
(Zingiber officinale) (11%) and sage (Salvia Officinalis) (8.2%). Linden was used for respiratory
diseases (85.71%) and lumber hernia (7.14%), and in order to strengthen the immune system (7.14%),
whereas ginger was used in the treatment of respiratory tract diseases. Sage-using participants
consumed this plant for the treatment of respiratory (66.6%) and gastrointestinal diseases (16.6%), and
as a sedative during menopause. The number of plants used by participants was compared with the
economic situation, sex, occupation and social security, and a difference was found between the
economic situation and the number of plants (p=0.023). Participants with moderate economic status
used 1-3 plants. Participants' preferences were asked about plants and medicines in order to use in the
treatment of diseases. All of the participants who did not have social security preferred to use the
plant. In addition, there was no significant difference between the number of plants and the preference,
but it was found that the people preferring to use plants used 4-10 plants. It was found that the
medicinal plants were widely used in the treatment of respiratory tract diseases by people participating
in the research, which may be due to the high prevalence of respiratory illnesses in cold climatic
conditions of Erzurum city. The use of medical plants by the peoples is of great importance for
reducing acute and chronic health problems and improving the quality of life.

KEYWORDS

Corresponding Author: SERAP BUZTEPE, seraparmut2009@hotmail.com


1
VOCATONAL SCHOOL OF HEALTH SERVCES, ATATURK UNVERSTY
2
MNSTRY OF HEALTH
3
MNSTRY OF FOOD, AGRCULTURE AND LVESTOCK

1693
Health, Medicinal plants, Erzurum

1694
Poster Session 13
Submission ID: 1693

LUPINUS ALBUS FUNCTIONAL CHARACTERISTICS AND


EFFECTS ON HEALTH

1 2
TAHA GKMEN LGER , FUNDA PINAR AKIROLU

ABSTRACT
Lupinus albus, a plant belonging to the family Fabaceae, is a herbaceous plant whose leaves
are split, cluster-like flowers and can grow up to 120 centimeters in length. There are about 200 kinds
of lupinus albus that grow wild in Europe, the Balkans and the Aegean and Mediterranean Regions in
our country. Lupinus albatus, lupinus anatolicus and lupinus angustifolius are the species that grow in
our country and are also called delicebakla, kurt baklas, termiye, yahudi baklas and gavur
baklas. The lupinus albus that matures in the autumn months is widely consumed especially in the
Mediterranean Region and Konya. Due to the alkaloids in its composition, the taste of the pellets is
bitter and its bitterness is eliminated with traditional methods (water retention and boiling) before
consumption. The lupinus albus that is consumed as a snack (tirmis) in winter usually has more
protein content than milled grains. Its content is high with regard to zinc, calcium, iron, potassium and
manganese which are considered micronutrient items. Although studies on the functional effects of
lupinus albus, which have very beneficial effects on health, have been limited, it has been reported that
it lowers blood glucose of diabetic rats in some clinical studies. The famous physician of the 11th
century, Ibn Sina, tried to treat diabetic patients with curcuma, lupinus albus and fenugreek. In
diabetic rats, it was observed that the lupinus albus added to their feeds decreased the
malondialdehyde (MDA) levels and increased the antioxidant enzyme levels. In hypercholesterolemic
subjects, it has been reported that the daily diet supplemented with lupinus albus reduced LDL
cholesterol levels. There are also data showing that it lowers blood pressure of hypertensive
individuals. More clinical studies are needed to address the functional effects of painful cilia, which
have a protective role in certain types of cancer and kidney diseases, particularly colon cancer.
Preventative health policies are important in obesity and type 2 whose prevalence is rapidly increasing
in the world and our country and which takes role in the etiology of many diseases, and these policies
may include functional foods such as lupinus albus. Considering the emergence of policies for the
development of healthy eating behaviors in health policies to prevent obesity and the prevalence of
type 2 diabetes, it may be helpful that individuals having interchangeable risk factors for type 2
diabetes (overweight and obesity, sedentary lifestyle, impaired fasting glucose and impaired glucose
tolerance, hypertension, low HDL cholesterol level, increased triglyceride level) consume foods such
as lupinus albus. Although some studies in the literature have shown that lupinus albus have
hypolipidemic and hypoglycemic effects, more studies are needed to determine the level of reliable
intake and other possible functional effects on health.

KEYWORDS
lupine, lupinus albus, hypolipidemic, hypoglycemic

Corresponding Author: TAHA GKMEN LGER, tgulger@ankara.edu.tr


1
ANKARA NVERSTES

1695
Poster Session 13
Submission ID: 1694

DEVELOPMENT OF MICROSATELLITE MARKERS FOR THE


WORLD'S MOST EXPENSIVE SPICE SAFFRON

1 ! 1
MEHMET KARACA , AYE GL NCE , EMINE UYGUR GER

ABSTRACT
Saffron, Crocus sativus Linn in the family Iridaceae, is a fall flowering perennial domestic
plant and its closed relative C. cartwrightianus, which originated in Crete, or in Central Asia. Saffron
is a sterile triploid plant and its stigmas are mainly used as a colorant for foodstuffs. Found in the
stigmas are apocarotenoid compounds responsible for the production of the yellow-orange color in
food. Stigmas also contain picrocrocin and safranal which produce a bitter taste and a hay-like
fragrance. These traits make saffron a much-sought ingredient in many foods worldwide. Saffron is
traditionally used against cancer, depressive mood, menstruation disorders, liver disease and pain. The
taxonomy of Crocus sativus is extremely complicated due to the lack of clear distinctive characters,
the wide range of habitats and the heterogeneity of the morphological traits and cytological data.
Although differences in the size and the number of the flowers produced by corms have been reported
previous studies utilizing a large number of markers obtained using 140 primers of random amplified
polymorphic DNA (RAPD), 91 primers of inter-simple sequence repeats (ISSRs) and 47 primer pairs
of simple sequence repeats (SSRs) could not differentiate among the forty three C. sativus from eleven
different countries including two from Turkey. Saffron, as a sterile plant, fails to produce viable seeds
and is thus dependent on vegetative reproduction. The existence of differences at the phenotypic level
such as size of the flowers, the shape of the tepals (combined sepals and petals), differences of color
and intensity in the tepals of saffron belonging to different regions of world may indicate genetic
differences. However artificial selection in the past may drastically reduce the genetic diversity.
Expressed sequence tag (EST) sequences are valuable resources for development of microsatellite
markers. The use microsatellite markers found transcribed and un-translated regions of the nuclear and
chloroplast genome of Crocus sativus could differentiate saffron varieties. These markers could be
also used in marker assisted selection studies for saffron breeding. In this study, a total of 6750 EST
sequences of Crocus sativus derived from publicly available databases of National Center for
Biotechnology Information (NCBI) were mined to obtain ESTs with microsatellites using Tandem
Repeats Analyzer 1.5 (TRA 1.5) program. Microsatellites in the present study were considered to
contain motifs that were between two and six nucleotides in length. The minimum motif length criteria
were defined as being 9 repeats for di-nucleotides, 7 repeats for tri-nucleotides, 6 repeats for tetra-
nucleotides and 5 repeats for penta-nucleotides and 4 repeats for hexa-nucleotides. Microsatellite
primer pairs flanking the microsatellite domains were designed using PRIMER3 software.
Microsatellite primer pairs were designed with several criteria such minimum/maximum as length of
expected product, GC content, melting temperature etc. A total of 30 EST-SSR primer pairs were
designed. Annealing temperatures of the primer pairs are set to 55 C (Tm 59 C) and expected to
amplify markers ranging from 209 bp to 334 bp in size. Thirty EST primers consisted of 5 di-
nucleotides, 7 tri-nucleotides, 6 tetra-nucleotides, 4 penta-nucleotides and 8 hexa-nucleotides. These

Corresponding Author: MEHMET KARACA, mkaraca@akdeniz.edu.tr


1
AKDENZ NVERSTES

1696
microsatellite primer pairs along with the conserved chloroplast microsatellite primer pairs could be
useful in genetic characterization and breeding studies of saffron.

KEYWORDS
Crocus sativus, EST-SSRs, genic microsatellites, primer development, SSRs

1697
Poster Session 13
Submission ID: 1698

PLANTS USED IN THE FOLK MEDICINE OF SORKUN TOWN


(BOZKIR / KONYA / TURKEY)

1 1
OSMAN TUGAY , DENZ ULUKU

ABSTRACT
Ethnobotanical studies which aim to shed light on local human-plant interactions in the
Sorkun Town (Bozkr / Konya), realized between 2016-2017 years. Into two years interviews were
made with about 11 informants and 30 medicinal uses were recorded. Some plants used as medicinal;
Abies cilicia (Ant.& Kotschy) Carr. subsp. isaurica Coode & Cullen, Achillea biebersteinii Afan.,
Alcea pallida Waldst. & Kit., Allium cepa L., Allium sativum L., Anthemis cretica L. subsp. albida
(Boiss.) Grierson, Anthemis pestalozzae Boiss., Anthemis tinctoria L. var. tinctoria, Cedrus libani
A.Rich., Cerasus avium (L.) Moench, Elaeagnus angustifolia L., Malva neglecta Wallr., Mentha
longifolia (L.) Huds. subsp. typhoides (Briq.) Harley var. typhoides, Pelargonium endlicherianum
Fenzl, Plantago lanceolata L., Plantago major L. subsp. major, Rosa canina L., Salvia adenocaulon
P.H.Davis, Sambucus ebulus L., Sesamum indicum L., Sideritis hispida P.H.Davis, Stachys cretica L.
subsp. anatolica Rech.fil., Stachys lavandulifolia Vahl var. lavandulifolia, Teucrium chamaedrys L.
subsp. syspirense (C.Koch) Rech.fil., Teucrium polium L., Thymus sipyleus Boiss. subsp. sipyleus
var. sipyleus, Urtica dioica L.

KEYWORDS
Medicinal plants, Sorkun, Bozkr, Konya

Corresponding Author: OSMAN TUGAY, otugay@selcuk.edu.tr


1
SELUK NVERSTES

1698
Poster Session 13
Submission ID: 1699

THE ROLE OF BIOTECHNOLOGY IN AMARILLADIACECA FAMILY

1 2
MRAN ENEL , M. ERCAN ZZAMBAK

ABSTRACT
The Amaryllidaceae family comprises more than 75 genera and 1100 species of herbaceous,
perennial and bulbous flowering plants. In this family there are important ornamental plants such as
Leucojum, Pancratium, Narcissus, Sternbergia and Galanthus. Some plants in this family also have
pharmacological value due to the important alkaloids they contain. Today, the use of biotechnological
tools is important for the sustainable production of these alkaloids. The need for these alkaloids used
in the treatment of some important diseases is increasing day by day and access to plant resources is
limited. For this reason, in vitro studies are considered as an alternative approach for alkaloids
production. In this study, in vitro studies on pharmacologically valuable plants in Amaryllidaceae
family were investigated.

KEYWORDS
Amaryllidaceae, alkaloids, in vitro

Corresponding Author: MRAN ENEL, ummsenel@gmail.com


1
EGE TARIMSAL ARATIRMA ENSTTS
2
EGE NVERSTES

1699
Poster Session 13
Submission ID: 1700

POISONOUS BOTANICALS OF VAN PROVINCE (TURKEY)

1 1 1
ABDULLAH DALAR , MUZAFFER MKEMRE , SEMA SEZGIN

ABSTRACT
Plants have been used as ethnobotanically by human for their medicinal, food, ornamental,
fuel, recreational and also poisonous properties since ancient times. The most ancient poisons also
come from plants such as Atropa sp., Nerium sp., Datura sp., Aconitum sp. and Hyoscyamus sp. Many
members of the plant kingdom have toxic compounds that can be highly poisonous, even lethal, if
ingested. At present, despite the enormous development of toxicology surveillance systems, poisoning
by plant samples continues to be one reason of morbidity and mortality. Within this presentation
poisonous plant samples grown in Van province including Anthriscus sp., Ferula sp., Vincetoxicum
sp., Euphorbia sp., Papaver sp., Aconitum sp., with their ethnobotanical data will be presented.

KEYWORDS
Poisonous, Van, Ethnobotany

Corresponding Author: ABDULLAH DALAR, dalar.abdullah@gmail.com


1
YZNC YIL NVERSTES

1700
Poster Session 13
Submission ID: 1701

ANTIOXIDANT ACTIVITY OF ANTHEMIS AUSTRIACA JACQ. AND


A. PSEUDOCOTULA BOISS. EXTRACTS AND THEIR TOTAL
PHENOLIC AND FLAVONOID CONTENTS

1 2 3
ZEKIYE CEREN ARITULUK , NEZIHA YAMUR KUMSER DKER , FFET REM TATLI ANKAYA

ABSTRACT
The genus Anthemis L. (Asteraceae) is represented by 81 taxa belonging to 51 species in the
Flora of Turkey1,2. Several species of Anthemis genus have been used as folk medicine in the
treatment of various health problems such as cold, fever, cough, bronchitis, stomachache, menstrual
problem and gastrointestinal disorders3-6. In this study, Anthemis austriaca Jacq. and A. pseudocotula
Boiss. used as folk medicines were investigated for their potential antioxidant activity. For this
purpose, methanol and aqueous extracts of the aerial parts of these plants were tested with three
complementary methods, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity,
2,2 azinobis (3 ethylbenzothiazolin 6 sulphonic acid) (ABTS) radical cation scavenging capacity
and cupric ion reducing antioxidant capacity (CUPRAC). Moreover, total phenolic contents of plant
extracts were determined by Folin-Ciocalteus reagent and their flavonoid contents were determined
by aluminum chloride colorimetric method. The results of present study revealed that, all plant
extracts exhibited antioxidant activity with various potencies. In the DPPH assay, both methanol and
aqueous extracts of A. pseudocotula (IC50=140.44 and 120.14 g/mL, respectively) showed higher
scavenging activity than methanol and aqueous extracts of A. austriaca (IC50=119.72 and 116.73
g/mL, respectively). Contrary to DPPH assay, both methanol and aqueous extracts of A. austriaca
showed higher scavenging activity against ABTS radical cation (153.53 and 181.09 mg Trolox
equivalents/g extract, respectively) compared with A. pseudocotula extracts (128.77 and 117.76 mg
Trolox equivalents/g extract, respectively). In the CUPRAC assay, the aqueous extracts of A. austriaca
and A. pseudocotula exhibited higher antioxidant activity (100.50 and 67.00 gallic acid equivalents/g
extract, respectively) than methanol extracts. In addition, the highest phenolic content was detected in
A. austriaca aqueous extract as 138.40 mg gallic acid equivalents/g extract, while the highest
flavonoid content was detected in A. pseudocotula methanol extract as 31.17 mg quercetin
equivalents/g extract. References: 1. Davis P.H. 1975. Flora of Turkey and the East Aegean Islands,
Vol.5, University Press, Edinburgh. 2. Gner A., Ozhatay N., Ekim T., Baser K.H.C. 2000. Flora of
Turkey and the East Aegean Islands, Vol. 11, University Press, Edinburgh. 3. Grdal B., Kltr S.
2013. An ethnobotanical study of medicinal plants in Marmaris (Mula, Turkey). Journal of
Ethnopharmacology, 146, 113-126. 4. Altundag E., Ozturk M. 2011. Ethnomedicinal studies on the
plant resources of east Anatolia, Turkey. Procedia Social and Behavioral Sciences, 19, 756-777. 5.
Bulut G., Tuzlaci E. 2013. An ethnobotanical study of medicinal plants in Turgutlu (Manisa-Turkey).
Journal of Ethnopharmacology, 149, 633-647. 6.Tetik F., Civelek S., Cakilcioglu U. 2013. Traditional
uses of some medicinal plants in Malatya (Turkey). Journal of Ethnopharmacology, 146, 331-346.

Corresponding Author: ZEKYE CEREN ARITULUK, cerenarituluk@hotmail.com


1
HACETTEPE NVERSTES

1701
KEYWORDS
Anthemis, antioxidant, total phenolic content, total flavonoid content

1702
Poster Session 13
Submission ID: 1702

PRODUCTION AND FOREIGN TRADE OF POPPY SEED IN TURKEY

1 ! ! 1
FIKRIYE YAZAR , ZOZAN KAYA , GIZEM YENER , ARZU SEER

ABSTRACT
PRODUCTION and FOREIGN TRADE OF HAHA in TURKEY A poppy is the source of
the crude drug opium which contains powerful medicinal alkaloids such as morphine and has been
used since ancient times as an analgesic and narcotic medicinal and recreational drugs. The sugared,
milled mature seeds are eaten with pasta, or they are boiled with milk and used as filling or topping on
various kinds of sweet pastry. The planting of opium poppy is supervised by the UN all over the
world. The UN designated Turkey, India, Australia, France, Spain, Hungary, Czech Republic and
China as the legal producers of opium poppy. Turkey and India are regarded as the traditional opium
poppy producer countries by the UN. Cultivation, production and trade of opium were free until 1933
in Turkey. In 1933, controlled poppy cultivation and production was launched and late on, in 1938
with the (ne demek) establishment of the Turkish Grain Board (TGB) and monopoly authority of
narcotic drugs was given to TGB. It was established as an Economic State Enterprise in order to
stabilize the grain market and operate the monopoly of opium and narcotic substances in Turkey. For
this purpose, being organized throuhout the country and within the framework of the relating
legislation as it has been defined TGB as traditional poppy producer. So, it has been responsible for
the cultivation, pracessing and marketing of poppy to be used for medical and scientific purposes in
order to meet the need both domestic and foreign market. The city of Afyonkarahisar in Turkey (afyon
"poppy, opium", kara "black", hisar "fortress") was a traditional center of poppy cultivation .
Afyonkarahisar is produce about 31 % Tukey production of poppy seeds in 2016 .World production of
poppy seeds is around 102.331 t and major producers are Turkey and Czechia Republic. These two
countries meet rough 52 % of the World demand. Turkey currently only produces about 26 % of the
world total. According to ten years ago poppy production was increaed in Turkey and World.
Approximately 95 % of our products are exported. This study aims at searching the production and
foreign trade and manifesting the marketing structure and studying the role of TGB in the market
structure of the poppy seeds.. Keywords: Poppy seed, foreign trade, TGB, production

KEYWORDS
Poppy seed, foreign trade, TGB, production

Corresponding Author: FKRYE YAZAR, fikriyeyazar@hotmail.com


1
UKUROVA NVERSTES

1703
Poster Session 13
Submission ID: 1705

COMPOSITIONS OF AMINO ACIDS IN DIFFERENT VARIETIES OF


SALEP TUBERS.

1 2 1 1 1
MEHMET FATIH CENGIZ , CEVDET GM , TIMUR TONGUR , TANER ERKAYMAZ , ONUR BASANELEBI ,
1
AYE KEVSER BILGIN

ABSTRACT
Salep is not cultivated on a commercial scale and is obtained by collecting tubers of the
Orchidaceae plants from the wild. Amino acid contents of this plant can be important in the area of
medicinal and healthcare sectors. In the study, amino acid compositions of six different varieties of
salep tubers (Himantoglossum robertianum, Orchis italica, Ophyrs ferrum-equinum,
Serapiasvomeracea, Orchissancta, Anacamptis pyramidalis), which were collected fromdifferent
regions of Turkey, were determined. Salep tubers were collected from their natural habitat. The tubers
were boiled in hot water, dried in sunlight and grinded by a laboratory type mill. Individual amino
acids (arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine,
phenylalanine, proline, serine, threonine, tyrosine and valine) were analyzed by liquid chromatography
tandem mass spectrometry (LC MSMS) without derivatization. The method was found to be selective,
linear (r2>0.99) and precise for all of interested amino acids. The limits of the quantifications (LOQ)
were found to be in the range from 0.53 to 1.20 mg/kg. The content of 15 amino acids quantified by
LC MSMS ranged between >LOQ-14419.91 mg/kg. The total amino acid was found to be 10129.29
mg/kg , 20029.61 mg/kg, 10483.00 mg/kg, 9510.92 mg/kg, 10921.48 mg/kg and 9825.68 mg/kg for
Orchissancta, Serapiasvomeracea, Ophyrs ferrum-equinum, Orchis italica, Himantoglossum
robertianum and Anacamptis pyramidalis, respectively. None of the analyzed samples was contained
cysteine. Lysine (2036.58 mg/kg), arginine (14419.91 mg/kg), histidine (458.41 mg/kg), serine
(551.25 mg/kg), proline (144.24 mg/kg), valine (261.07 mg/kg), methionine (26.37 mg/kg), tyrosine
(425.50 mg/kg) and phenylalanine (537.21 mg/kg) amino acids were detected in Serapiasvomeracea
samples. The highest concentration of aspartic acid and glutamic acid , Ophyrs ferrum-equinum. The
content of leucine + isoleucine (168.40 mg/kg) was the highest in Orchis italica, In conlusion,
Serapiasvomeracea has the most valuable salep variety in tested samples due to its high content of
amino acids. Therefore, it can be preferred in medicinal and healthcare treatment which amino acid
compositions is accepted as important.

KEYWORDS
Amino acids, Composition, Salep tubers

Corresponding Author: MEHMET FATH CENGZ, fcengiz@akdeniz.edu.tr


1
AKDENZ UNVERSTES
2
BARTIN NVERSTES

1704
Poster Session 13
Submission ID: 1706

ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC AND FLAVONOID


CONTENTS OF TWO ENDEMIC SIDERITIS L. TAXA FROM TURKEY

1 1 1
NEZIHA YAMUR KUMSER DKER , ZEKIYE CEREN ARITULUK , FFET REM TATLI ANKAYA

ABSTRACT
The genus Sideritis L. belonging to Lamiaceae family comprises more than 150 species
worldwide, most of which are mainly found in the Mediterranean area. In Turkey, Sideritis genus is
represented by 53 taxa and 39 of those are endemic1. Several species of Sideritis genus have been
used as tea because of their pleasant aroma and special taste as well as their healing effects. The
members of this genus are rich in essential oil, flavonoids and diterpenes which are responsible for
their biological activities such as antiinflammatory, antimicrobial, antiulcerogenic, antioxidant,
analgesic etc.2 In this study, methanol and aqueous extracts of the aerial parts of Sideritis libanotica
Labill. ssp. linearis (Benth.) Bornm. and S. phrygia Bornm. were investigated for their potential
antioxidant activity by three complementary methods, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH)
radical scavenging capacity, 2,2 azinobis (3 ethylbenzothiazolin 6 sulphonic acid) (ABTS)
radical cation scavenging capacity and cupric ion reducing antioxidant capacity (CUPRAC).
Moreover, total phenolic contents of plant extracts were determined by Folin-Ciocalteus reagent and
their flavonoid contents were determined by aluminum chloride colorimetric method. The results of
present study revealed that, all plant extracts exhibited antioxidant activity with various potencies. The
methanol extracts of S. libanotica ssp. linearis and S. phrygia showed higher scavenging activity
against DPPH radical (IC50=149.23 g/mL and 131.78 g/mL, respectively) and against ABTS
radical cation (185.91 and 149.84 mg Trolox equivalents/g extract, respectively) while the aqueous
extracts showed higher activity on CUPRAC assay (58.71 and 57.28 gallic acid equivalents/g extract,
respectively). Total phenolic contents of extracts were in range between 92.47 and 138.09 mg gallic
acid equivalents/g extract and the total flavonoid contents were in range between 17.60 and 29.50 mg
quercetin equivalents/g extract. References: 1. Gven A., Houghton P.J., Duman H., Cokun M.,
ahin P. 2005. Antioxidant activity studies on selected Sideritis species native to Turkey.
Pharmaceutical Biology, 43(2), 173-177. 2. Gonzles-Burgos E., Carretero M.E., Gmez-Serranillos
M.P. 2011. Sideritis spp.: Uses, chemical composition and pharmacological activities-A review.
Journal of Ethnopharmacology, 135, 209-225.

KEYWORDS
Sideritis, antioxidant, total phenolic content, total flavonoid content

Corresponding Author: ZEKYE CEREN ARITULUK, cerenarituluk@hotmail.com


1
HACETTEPE NVERSTES

1705
Poster Session 13
Submission ID: 1707

NATURAL PLANTS USED AS MEDICINAL IN KOZLUCA (ACIGL /


NEVEHR) VILLAGE

1 2
DENIZ ULUKU , OSMAN TUGAY

ABSTRACT
This study was carried out to determine the natural plants used as medicines in Kozluca
Village (Acgl / Nevehir). Field visits were organized between March and August months in 2015-
2016. A total of 28 plant samples were taken from interviews with 20 source people in the field
surveys conducted and information on how these plants were used as medicines were noted. In the
field studies conducted, pictures of the plants in the field were taken. According to the common
herbarium techniques, plants were pressed and dried. To determine the scientific names of plants were
used Flora of Turkeyas main source. In addition, local names are written by using the local people. As
a result of evaluation of plant samples collected from Kozluca Village, t was determined that total 28
natural plants belonging to 13 families and 25 genera were used as medicines. Some of these plants;
Capsella bursa-pastoris (L.) Medik., Gundelia tournefortii L., Lactuca sativa L., Malva neglecta
Wallr., Scorzonera cinerea Boiss., Tragopogon dubius Scop., Tribulus terrestris L., Papaver rhoeas L.,
Plantago major L., Urtica dioica L., Portulaca oleracea L.

KEYWORDS
Ethnobotanic, medicinal plants, Kozluca, Nevehir.

Corresponding Author: DENZ ULUKU, ulukusdeniz@gmail.com


1
SELUK UNVERSTY, DEPARTMENT OF BOTECHNOLOGY, FACULTY OF SCENCES
2
SELUK UNVERSTY, DEPARTMENT OF BOLOGY, FACULTY OF SCENCES

1706
Poster Session 13
Submission ID: 1708

THE DETERMINATION OF SOME PHYTOCHEMICALCONTENTS


AND ANTIOXIDANT ACTIVITIES OF ECHINACEA PURPUREA
VARIETIES CULTIVATED IN TURKEY

1 1
SADYE AYE ELK , YKSEL KAN

ABSTRACT
In this study, three different Echinacea purpurea varieties were cultivated and carried out in
the Ecological Conditions of Konya in 2013 and 2014 in Seluk University Faculty of Agriculture
Agricultural and Aromatic Herb Farms. These varieties are Echinacea purpurea var. purpurea,
Echinacea purpurea var. baby white swan and Echinacea purpurea var. double decker. These varieties
are named according to flower arrangements as Echinacea purpurea var. purpurea pink coneflower,
Echinacea purpurea var. baby white swan white coneflower and Echinacea purpurea var. double
decker double coneflower. These varieties are harvested in 2013 and 2014 after the start of flowering
period, full flowering period and seed binding period, then they are separated in to leaves, stemmed
flowers, herbs and roots and dried to a certain moisture content. At the same time, samples were taken
from the roots during the period of root harvest (October-November) except for other three period.
The contents of ash, caffeic acid derivatives (caftaric and chicoric acid), antioxidant activity (DPPH
method), total phenol content, total flavonoid content were determined in the all plant parts of each
varieties taken at three different harvesting periods. In addition, volatile oil content and volatile oil
components were also detected in herbs taken during the full flowering period. The highest essential
oil is obtained from Echinacea purpurea var. baby white swan (0.3%). When looked at the essential oil
components of three different Echinacea varieties, the components found in all of them are
caryophyllene oxide and germacrene D. The highest ratio of Germacrene D was determined in
Echinacea purpurea var. purpurea herb (% 21.563). According to the average of all values the highest
amount of caffeic acid was obtained from E. purpurea var. purpurea (% 0.995) and leaf (% 0.696). The
highest amount of chicoric acid was obtained from E. purpurea var. purpurea root (% 2.589).
According to different harvesting times, Echinacea species should be harvested in full flowering
period for the highest amount of chicoric acid. Total flavonoid content was determined highest in
leaves (at the leaves of Echinacea purpurea var. double decker 25,0844 mg/g extract in 2014 leaf
samples). The total phenol content was determined highest in leaves (at the leaves of Echinacea
purpurea var. double decker 29,4378 mg/g extract in 2013 leaf samples). Antioxidant activity values
were found to be in all varieties.

KEYWORDS
Echinacea purpurea, Caffeic acid derivatives, Essential oil, Antioxidant activity.

Corresponding Author: SADYE AYE ELK, sacelik@selcuk.edu.tr


1
SELUK NVERSTES

1707
Poster Session 13
Submission ID: 1709

MEDICINAL HERBS PREVENTING OXIDATION OF BUTTER*

1 2 2 3 4
UUR KARDIL , MURAT KK , SEMRA ALKAN TRKUAR , HACER DOAN , AYA AKTA KARAELIK

ABSTRACT
In terms of food technology, probability of the fats and the products rich in fat to be oxidized
is very high. Characteristic features such as flavor, color, tissue, odor and nutritional value of these
oxidized products deteriorate. Antioxidants are known to prevent these deteriorations which ocur as a
result of oxidative rancidity and oxipolymerization. The chemicals used in foods as antioxidant have
many harmful effects to health. The use of natural antioxidants will be more meaningful in both
preventing these harmful effects and oxidations. For this purpose, some plants were obtained and their
aqueous and water-saturated ethyl acetate extracts were prepared. The selected plant extracts were
added to the butter at 200 ppm concentration. In addition, the reference samples were prepared by
adding BHT and Trolox standards to butter samples at 200 ppm concentration. In order to follow the
oxidation in the butter samples prepared, TBA (thiobarbituric acid assay), PV (perdoxide value) and
FFA (free fatty acid) analyses were applied. The results obtained by the TBA test is shown in figure 1.
All the plant extracts and standarts successfuly prevented oxidation in butter when compared to the
control. Especially performance of UKS18 and UKE20 coded samples in protection against oxidation
of butter is pretty good compared to the synthetic antioxidants. The results of the analyses of the three
methods revealed that many of the plant extracts and especially UKS18 and UKE20 can be used as a
natural antioxidant in order to prevent oxidation of butter. *This study is supported by 2210-C Priority
Areas Domestic Master Scholarship Programme of TUBITAK-BIDEB.

KEYWORDS
butter, oxidation, natural antioxidant

Corresponding Author: UUR KARDL, ugurkardil_61@hotmail.com


1
ADEPARTMENT OF GENETCS AND BOENGNEERNG, FACULTY OF ENGNEERNG AND NATURAL
SCENCES, GMHANE UNVERSTY, 29100 GMHANE, TRKYE
2
BDEPARTMENT OF CHEMSTRY, FACULTY OF SCENCES, KARADENZ TECHNCAL UNVERSTY, 61080
TRABZON, TRKYE
3
BHTT UNVERSTY SCENTFC AND TECHNCAL APPLCATON AND RESEARCH CENTER (HBTUAM),
HTT UNVERSTY, 19030 ORUM, TRKYE
4
CDEPARTMENT OF FOOD PROCESSNG, ESPYE VOCATONAL SCHOOL, GRESUN UNVERSTY, 28000
GRESUN, TRKYE

1708
Poster Session 13
Submission ID: 1710

DETERMINATION OF FIXED FAT COMPONENTS OF THE


NATURALLY GROWING COCKLEBUR (XANTHIUM SP. L.) PLANT
IN KAHRAMANMARA

1 1 1 1 2 3
ALI RAHMI KAYA , OSMAN GEDK , MER SHA USLU , ADEM EROL , TAMER ERYGT , CNEYT CESUR ,
4
AHMET KILIN

ABSTRACT
Xanthium L. is a plant belonging to the Asteracea family and it is grown almost in every
region of the world. It is found mostly in tropical and subtropical regions (Weaver and Lechowich
1982). It is common in America, Canada, Mexico, Malaysia, Indonesia and India (Alam et al., 2011).
To date, many taxonomic studies have been carried out on this plant. Caius (1986) reported 25 species
of Xanthium genus, and Weaver and Lechowich (1982) reported 20 species (Alam et al., 2011). It is
represented in Turkey by 6 taxa including 3 species and 3 subspecies (Gner et al., 2012, Cesur and
enkal, 2015). According to Cesur and enkal (2015), Xanthium sp. plants are accepted as weeds in
Turkey today. Again, according to the same researchers, the plant is a plant that grows in anhydrous
areas, with approximately 25% crude oil in its seed, which represents an important potential for
exploring the possibility of obtaining oil. In this research, a number of studies have been done within
the scope of the mentioned evaluations above. First, seed samples belonging to cocklebur plant
(Xanthium sp.) were collected from around Kahramanmaras Menzelet Dam Lake. Then fixed fatty
acid components were examined in collected seed samples. It has been determined that there are
totally twenty two different fixed fatty acid components in cocklebur. The main fixed fatty acid
components and ratios are; Linoleic acid (75.23%), oleic acid (13.59%), palmitic acid (5.93%), stearic
acid (3.57%), behenic acid (0.68%) and myristic acid (0.02%).

KEYWORDS
Cocklebur, Xanthium sp., Fixed oil, Fatty acid components, Kahramanmaras

Corresponding Author: AL RAHM KAYA, alirahmikaya@ksu.edu.tr


1
KS ZRAAT FAKLTES
2
YZNC YIL NVERSTES GEVA MESLEK YKSEKOKULU
3
BOZOK NVERSTES ZRAAT FAKLTES
4
KS ZRAAT FAKLTES

1709
Poster Session 13
Submission ID: 1712

WATER-SATURATED ETHYL ACETATE EXTRACTS OF SOME


HERBS PROMOTE BUTTER OXIDATION*

1 2 3 3 5
UUR KARDIL , HACER DOAN , MURAT KK , SEMRA ALKAN TRKUAR , AYA AKTA KARAELIK

ABSTRACT
Lipid oxidation of fats and fat containing foods deteriorate their characteristic properties such
as flavor, color, texture, odor and nutritional value during manufacturing and storage. Therefore,
various synthetic antioxidants are used for a long time as food additives to increase the oxidation
stability of fats. Reliability of synthetic antioxidants has created a dispute. Therefore consumers,
owing to their positive effects on health such as high antioxidant activity, desire the use of natural
additives in the food industry instead of synthetic ones. Recently, many plant extracts have been
investigated in terms of antioxidant and antimicrobial activity and the results have been compared to
controls and standard antioxidants. In this study, plant extracts obtained with ethyl acetate saturated
with water having high antioxidant activity were investigated to determine their suppressive effect on
oxidation of butter. Concentrations of the selected plant extracts added to the butter were 200 ppm.
Control sample (containing no antioxidants) was also used. In order to monitor the oxidation of the
sample determination of peroxide value (PV) was utilized during a 180 days storage at 25 C (Figure ).
In contrary to our expectation, the extracts promoted the oxidation of butter leading to higher peroxide
values as compared to that of control. The results provide an important fact about the food
preparations of fatty nature that the plants to be used in such preparations should be closely monitored
for their possible oxidation promoting properties besides the beneficial ones. *This study is supported
by 2210-C Priority Areas Domestic Master Scholarship Programme of TUBITAK-BIDEB.

KEYWORDS
butter, oxidation, natural antioxidant

Corresponding Author: HACER DOAN, hacerdogan@hitit.edu.tr


1
ADEPARTMENT OF GENETCS AND BOENGNEERNG, FACULTY OF ENGNEERNG AND NATURAL
SCENCES, GMHANE UNVERSTY, 29100 GMHANE, TRKYE
2
HTT UNVERSTY SCENTFC AND TECHNCAL RESEARCH CENTER (HBTUAM), HTT UNVERSTY,
19030 ORUM
3
BDEPARTMENT OF CHEMSTRY, FACULTY OF SCENCES, KARADENZ TECHNCAL UNVERSTY, 61080
TRABZON, TRKYE
4
CDEPARTMENT OF FOOD PROCESSNG, ESPYE VOCATONAL SCHOOL, GRESUN UNVERSTY, 28000
GRESUN, TRKYE

1710
Poster Session 13
Submission ID: 1714

ANTIOXIDANT AND ANTIMICROBIOLOGICAL ACTIVITY OF


HIBISCUS SABDARIFFA

1 1
ELIF ELIK , ZLEM TURGAY

ABSTRACT
ANTIOXIDANT AND ANTIMICROBIOLOGICAL ACTIVITY OF Hibiscus sabdariffa Elif
ELK, zlem TURGAY University of Kahramanmara St mam, Faculty of Engineering and
Architecture Department of Food Engineering ozlem@ksu.edu.tr Hibiscus sabdariffa, Malvaceae,
commonly known as roselle, red sorrel or karkade, is an annual herbaceous. These calyces are a good
source of natural food colorants because of their high pigment content and the dried calyces are
consumed worldwide in hot infusions and in cold drinks. In this study, total phenolic content, gallo-
catechin content and antibacterial activity of the water extracts of Hibiscus sabdariffa were researched.
Total phenolic content of water extracts of dry calyces was determined by the Folin-Ciocalteu method.
The absorbance was measured at 1, 2, 5, 10, 20, 30, 60, 90 min and performed extraction kinetics.
Gallo-catechin content was detected by HPLC-DAD. Antimicrobial activity of volatile fatty acid of
Hibiscus sabdariffa was determined by agar gel diffusion method against Escherichia coli and
Staphylococcus aureus. The total phenolic contents were ranged between 771-2056 mg gallic acid
equivalents (GAE)/100 ml hibiscus tea and gallo catechin contents were ranged between 0- 4.6 ppm.
Tobramycin(10 g), cefuroxime(30 g), tetracycline (30 g), cephazolin (30 g), bacitracin (0,04
unit), nalidixic acid (30 g), nitrofurantoin (300 g) were used as positive control. Water extracts of
Hibiscus sabdariffa showed 7 mm inhibition zone against Escherichia coli and 10 mm inhibition zone
against Staphylococcus aureus.

KEYWORDS
Hibiscus sabdariffa, volatile fatty acid, extraction kinetics

Corresponding Author: ELF ELK, elifcelik@ksu.edu.tr


1
KAHRAMANMARA ST MAM NVERSTES

1711
Poster Session 13
Submission ID: 1715

DETERMNATON OF BOLOGCAL ACTVTY OF SOME


EXTRACTS AUBRETA DELTODEA

1 1
NAHDE DENZ , ZEK ARICI, RAMAZAN MAMMADOV

D
ABSTRACT
Since ages, literature has revealed plants to be the most important source of medicines for
human health . According to the findings of the World Health Organization, up to 80% of the worlds

TE
population relies on plants for their primary health care.. The Brassicaceae family is used as a
landscape plant of the genus Aubrieta. There are a few taxonomic, morphological, anatomical and
ecological studies carried out with species belonging to the genus Aubrieta in our country.The
majority of the work done is in the economic and landscape areas. In the study, Aubrieta deltoidea
EN
belonging to genus Aubrieta which is distributed in Denizli belonging to Brassicaceae family was
used. Diagnosis of the plant was made according to Davis (1978). Plant samples were collected in July
2015, dried and ready for analysis. Plant samples were stored at +4C until analysis runs. In this study,
some biological activities of ethanol and acetone solvent extracts of Aubrieta deltoidea, which are
naturally distributed in our country, have been investigated.Biological activity of species; -carotene-
ES
linoleic acid method and the DPPH method.The highest antioxidant activity (83.33%) was seen in
ethanol extract of A. deltoidea strain.The highest free radical scavenging activity (81.24%) was seen in
the A. deltoidea strain extract prepared with the acetone solvent. The results obtained in this study
have shown that plants can be used as a readily available natural antioxidant source. A more detailed
PR

and versatile survey of these plants will be of great benefit to my country in terms of public health.

KEYWORDS
A. deltoidea , biological activity, DPPH, -carotene-Linoleic acid method
T
O
N

Corresponding Author: NAHDE DENZ, ndeniz_09@hotmail.com


1
PAMUKKALE NVERSTES

1712
Poster Session 13
Submission ID: 1717

COMPOSITION OF HELICHRYSUM ITALICUM (ROTH) G. DON


ESSENTIAL OIL FROM CYPRUS

1 1 2 2
BETL DEMIRCI , GLSM YILDIZ , MEHMET KOYUNCU , CNEYT K. BUHARALIOLU , KEMAL HSN CAN
3
BAER

ABSTRACT
The genus Helichrysum Mill. (Asteraceae) comprises nearly 185 genera and 1240 species
worldwide (Europe, Asia, Africa and Madagascar)( Bayer et al., 2007). It is represented by 24 species
and 30 taxa of which 17 are endemic in the flora of Turkey (Guner et al.,2012). Helichrysum species
are commonly known as lmez iek, altnotu, yayla iei and are generally used as herbal tea in
Turkey (Baytop, 1997). Helichrysum species are used in folk medicine for removing kidney stones
(Suzgec et al., 2005,). Essential oil of Helichrysum italicum has antibacterial and anti-fungal activity
(Mastelic et al., 2005). The study material was collected from Cyprus in May 2015. Aerial parts of
Helichrysum italicum were subjected to hydrodistillation for 3 hours using a Clevenger apparatus.
Chemical composition of the oil was investigated with GC-FID and GC/MS techniques. In total, 75
compounds (88.4%) were identified. The main components were -caryophyllene (12.1%),
tetradecanoic acid (10.9%), hexadecanoic acid (10.1%), -humulene (7.9%), pentacosane (7.0%),
dodecanoic acid (4.5%), rosifoliol (3.3% ) and heptacosane (3.2%). Key words: Helichrysum italicum,
essential oil, GC/MS, GC-FID.

KEYWORDS
Helichrysum italicum, essential oil, GC/MS, GC-FID.

Corresponding Author: GLSM YILDIZ, gulsumyildiz@anadolu.edu.tr


1
ANADOLU NVERSTES
2
ULUSLARARASI KIBRIS NVERSTES
3
KIBRIS YAKIN DOU VERSTES

1713
Poster Session 13
Submission ID: 1718

PRODUCING DISEASEFREE GINGER IN GREENHOUSE CULTURE


IN ANTALYA

1 1
AYE GL NCE , MEHMET KARACA

ABSTRACT
Culinary ginger (Zingiber officinale Rosco) belongs to the family Zingiberaceae. Rhizomes of
ginger are valuable not only as a spice but also as herbal medicine in the world. The production of
culinary ginger is constrained due to: (i) it has a low proliferation rate; (ii) it is easily infected by soil-
borne pathogens such as bacterial wilt, soft rot and nematodes, which cause heavy losses in yield in
open-field production; (iii) it has poor flowering and seed sets. Culinary ginger is tropical plant and
needs plenty of heat and humidity. Climate of Antalya is not suitable for of culinary ginger. In this
study, fresh culinary ginger rhizomes purchased from a grocery store in Antalya was used as starting
plant material to investigate the possibility of producing diseasefree ginger in greenhouse culture in
Antalya. In the second year, a total of 12 rhizomes were used as planting materials. Rhizomes (the
seed-pieces) had 2-4 well developed growth buds ("eyes"). Rhizomes are surface-sterilized in a 10%
solution of household bleach (1 part bleach in 9 parts water) for 10 minutes, washed with ddH2O and
then cured in a clean, disease-free area for three days before planting. Rhizomes were planted just
below the soil surface in 12 pots filled with 1/4 peatmoss, 1/4 vermiculite, 1/4 perlit and 1/4 garden
soil. Pots were watered sparingly until top growth developed. Once established, seedlings were
watered heavily, fertilize monthly, and kept in a partially shaded location in a greenhouse. Harvesting
of the rhizomes was performed when the greenhouse temperature started to drop below 10oC.
Provided with adequate space, ginger plants could reach a height of 60-120 cm in the pots. Rhizomes
yield per pot ranged from 400 g to 1200 g with a mean of about 600 g. Extension of growth period
would increase the yield since during the harvesting time most of the ginger plants were in green. Our
yield values were obtained in 8 months started in late March and ended in early November.
Greenhouse production of ginger provided secured and protected environment from weather
throughout the growing season, reducing the potential for accidental introduction of diseases. Ginger
production was unitized, in each pot, allowing for quick removal from the area of a plant suspected
of being contaminated. Grow-bags instead of pots could also be used for ginger production which
could reduce the cost. Production in the pots filled with light-weight medium as the plants grow to
simulate the hilling cultivation done in the field, eliminating the potential for root injury as an entry
point for diseases. We noted that watering, fertilization and hilling of ginger production are very
important. Use of pots provides for easy watering, fertilization and hilling, harvesting, and cleaning. In
some hot days the temperature of the greenhouse exceeds 50oC and in these days plenty of water was
supplied to the floor of the greenhouse to reduce the heat stress. Pots were placed on benches which
were clean and at least 40 cm off the ground. Each pot was watered at least one time a day but in very
hot days watering times increased. Triple superphosphate, gypsum (CaSO4.2H2O), NPK fertilizer and
minor elements were provided. Based on 3 year-experiments the following conclusions were drawn:
(i) ginger seed-pieces should be planted during March and harvested as latest as possible for best
production; (ii) rhizomes with at least three eyes should be planted about 5 cm deep in each bag or pot

Corresponding Author: AYE GL NCE, aince@akdeniz.edu.tr


1
AKDENZ NVERSTES

1714
containing about 30 L of growing medium; (iii) hilling should be performed at least 4 times per
growing seasons; (iv) irrigation emitters should be placed on top of the growing medium and around
the planted seed-piece; (v) as the ginger plant grows, more medium should be added (hilling), and the
irrigation emitters should be moved to the top of the growing medium. We also noted that hilling was
an important process in ginger production to ensure development of rhizome size and mass. In the
main text further information and discussions are provided for producing diseasefree ginger in
greenhouse culture in Antalya.

KEYWORDS
Culinary ginger, disease-free growth, greenhouse production, Zingiber officinale

1715
Poster Session 13
Submission ID: 1719

TRANSFER OF MICROSATELLITES TO CARNATION USING INTER-


GENERA EST CONTIGS APPROACH

1 1
AYE GL NCE , MEHMET KARACA

ABSTRACT
Dianthus L. is one of the highly valued plant genera in the family Caryophyllaceae. The genus
contains both annual and perennial plant species most of which are valued for their ornamental,
volatile, aromatic, and medicinal properties. Compared with other ornamental, medicinal and aromatic
plant species, application of DNA-based molecular marker system is limited in carnation (Dianthus
caryophyllus L.). Amongst DNA markers, microsatellites; also known simple sequence repeats (SSRs)
or simple tandem repeats (STRs), are markers of the choice in many plant species. Microsatellites are
important for research in both basic and applied sciences. The development of microsatellite markers
require: (i) microsatellite library construction from genomic DNA of target species; (ii) identification
of unique microsatellite loci screening with single or mixed simple sequence oligonucleotide probes;
(iii) identifying a suitable DNA sequences flanking the microsatellite regions for primer design by
sequencing studies; (iv) identification of PCR products representing desired microsatellite alleles; (v)
evaluation and interpretation of microsatellite patterns, removing redundant and/or chimeric
sequences, and (vi) assessing PCR products for polymorphism. Alternatively expressed sequence tags
(EST) sequences are used in microsatellite markers development. However some species do not have
enough EST resources including Dianthus L. At the time of EST minding of the present study there
were just 630 ESTs for Dianthus L in NCBI databases. These are very low numbers of ESTs for
mining microsatellites. Thus this study used a new approach, called inter-genera EST contigs approach
to use the ESTs of related genera for microsatellite markers development. In this study, a total of 4618
EST sequences, 630 of which were for Dianthus caryophyllus L., 139 of which were Gypsophylla
arrostii Guss., and 3849 of which were Sileneae latifolia Poir. derived from publicly available
databases of National Center for Biotechnology Information (NCBI) were mined to obtain ESTs with
microsatellites using TRA 1.5 software program. Microsatellites in the present study were considered
to contain motifs that were between two and six nucleotides in length. The minimum motif length
criteria were defined as being 9 repeats for di-nucleotides, 7 repeats for tri-nucleotides, 6 repeats for
tetra-nucleotides and 5 repeats for penta-nucleotides and 4 repeats for hexa-nucleotides. In order to
obtain inter-genera contigs, ESTs containing microsatellites were assembled into contiguous
sequences (contigs) using the Sequencher software. Contigs assembly parameters were set to a
minimum overlap of 50 bases, 95% identity match, and the large gap option was implemented. A total
of 14 contigs consisting of microsatellite-ESTs from D. caryophyllus, G. arrostii, S. latifolia and 25
singletons (those ESTs that were not found in any contigs) were further analyzed to design
microsatellite primer pairs using the Primer 3 program. A total of 39 primer pairs were identified and
commercially synthesized. Genomic DNA samples of several commercial carnation cultivars were
extracted and amplified using the developed primer pairs. Results indicated that majority of the primer
pairs could be successfully used in production of microsatellite markers in carnation genetic studies.

Corresponding Author: AYE GL NCE, aince@akdeniz.edu.tr


1
AKDENZ NVERSTES

1716
KEYWORDS
Dianthus caryophyllus L., ESTs, Gypsophylla spp., Sileneae spp., SSR

1717
Poster Session 13
Submission ID: 1720

MEDICINAL PLANTS ARE USED BY MOTHERS TO COPE WITH


THE COMMON SYMPTOMS IN CHILDHOOD

1 2 1
SEVN POLAT , AYE GROL , AYE ENER TAPLAK

D
ABSTRACT
Introduction: In recent years, there has been an increasing interest in the use of complementary

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and alternative medicine all over the world. It is reported that about 40% of the health services is
composed of traditional medicine in China and 71% of the population in Chile and 40% of the
population in Colombia use similar medicine methods. Today, the interest towards the herbal methods
has gradually increased due to the increase of resistant strains of microorganisms against drugs, the
adverse effects of drugs, and their high cost. However, failure to know exactly the side effects of the
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plants consumed unconsciously has also brought the concerns about the harms of these methods. The
use of herbal methods widely by the parents in symptom management in their children with the
opinion that it is less harmful than drugs is an extremely sensitive situation in terms of the fact that it
can cause serious conditions that may result in death. This descriptive study was conducted to
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determine the medicinal plants are used by mothers to cope with some problems frequently
encountered in their children. Material and Method: The sample of the study consisted of 202 mothers
who applied to Family Health Centers located in a city center in Central Anatolia between January and
March 2017 and agreed to participate in the study. A questionnaire prepared by the researchers in
accordance with the literature was used to collect the data. The obtained data were evaluated by using
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descriptive statistical methods in IBM SPSS Statistics 21 packaged software in the computer
environment. Results: It was determined that average age of the mothers participating in the study was
38.4010.03, 35.1% of them were high school graduate and 64.9% of them were housewives. It was
determined that 41.5% of the mothers used herbal methods for sore throat induced by influenza and
cold, 37.1% for nausea-vomiting, 28.2% for gas pain and indigestion, 26.7% for constipation, 17.8%
for cough, 12.3% for coping with sleeping problems and calming the child down. It was found that
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almost all mothers preferred mint and lemon tea for coping with the sore throat induced by influenza
and cold in children, the mostly used herbal method to relieve the gas pain in children was the fennel
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tea, half of mothers used linden and ginger tea and majority of the mothers preferred fennel tea for
their children with constipation problems. 43.0% of the mothers were determined to obtain the herbal
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teas from sellers of medicinal herbs. 70.3% of the mothers considered that using herbal methods in
symptom management in children is useful and 62.4% also recommended the method they used to the
others. Conclusion: It was determined in the study that herbal teas were randomly used by the mothers
for their children without medical advice, more than half of the mothers preferred herbal methods in
symptom management and one third of the mothers recommended these methods to others. In
accordance with the results obtained from the study, it is recommended to raise awareness of mothers
about this matter by healthcare professionals and inform them about the appropriate dose and usage of

Corresponding Author: AYE GROL, ayseparlak42@gmail.com


1
BOZOK NVERSTES
2
ATATRK NVERSTES

1718
the herbal teas suggested by the physician in order to protect children from the detrimental effects of
the herbal methods.

KEYWORDS
Alternative theraphy, Child, Sympthom, Medicinal plants

1719
Poster Session 13
Submission ID: 1721

A NEW CONCEPT: NUTRACEUTICALS AND THEIR CONTENT

1 1 1 2
NAZAN AKTA , GLPER HAKLI , ESMA AYBATTI , BLN ALTUN

ABSTRACT
Throughout life, people aim to maintain a healthy and high quality life. For maintaining a
healthy lifestyle, feeding with quality foods is essential. The purpose of this study is to examine the
nutraceutical conception that emerged as a result of combining of the words: nutrition and
pharmaceutical. In this review study, the secondary data from the primary researches in the same
subject were used, these data were obtained as a result of scanning in electronic scientific databases as
well as written sources. Nutraceutical term, which provides a protective or physiological benefit to a
chronic disease as an extract or food was used for the first time in 1989 by Dr. Stephen De Felice.
While nutraceutical concept express any food extract support that enhances quality of life, maintains
and improves physiological well-being by protecting health, protective against chronic diseases, does
not develop toxic effects, functional foods refer to nutrients that reduce disease risk and have a
beneficial effect on health. Nutraceuticals and functional food terms are used to describe food or food
compounds that provide health benefits on the basic nutrition. While nutraceutical refers to both
traditional and diverse (tablet, capsule, etc.) food and food compounds, functional food refers to
traditional food forms. Functional food is called nutraceutical, when it helps the prevention and/or
treatment of other diseases and/or disorders except anemia. For daily life and health, nutrition; for
treatment of diseases, drugs; and for preventing diseases, nutraceuticals are required. Commonly used
nutraceuticals include carotenoids, antioxidant vitamins, phenolic compounds, terpenoids, steroids,
indoles, glycomine, controitin and fibers. Products that are used as nutraceuticals may be present in
different dosage forms such as tablets, capsules, soft gels, and are not considered drugs. It is expected
that the nutraceutical products that constitute a new field of interest in the science of pharmacognosy
will make frequent use, contribute to food and health care.

KEYWORDS
Nutraceutical, food, extract, nutrition

Corresponding Author: NAZAN AKTA, naktas@selcuk.edu.tr


1
SELUK NVERSTES
2
DR. ARAZ TIP MERKEZ

1720
Poster Session 13
Submission ID: 1722

THE EFFECTS OF LYCOPEN APPLICATION ON SODIUM


FLUORIDE (NAF) APPLIED RENAL CELL LINE

1 2 3 1 4
SEDAT ETN , FATMAGL YUR , MEHMET TAPINAR , SEMIHA DEDE , VEYSEL YKSEK

D
ABSTRACT
The present study was planned to investigate the potential protective effect of lycopene, an

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antioxidant carotenoid, on NaF applied renal cell line. NRK-52 cells were cultured under standard in
vitro conditions with regular passages. 10.000 cells were planted in each culture platelet well for
NRK-52E cells. Cells were incubated for 24 hours at 37C in an incubator that contained CO2. After
the incubation, the medium on the cells was removed and the prepared NaF and lycopene solutions
were added. At least 4 wells were used for each dose. Culture vessels were incubated at 37C in a CO2
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incubator for 6, 12, and 24 hours so that MTT stain could transform non-soluble formazan crystals and
MTT assay was conducted. Findings: In conclusion, it was found that low lycopene doses reduced the
toxic effect of NaF by 10-20%, whereas in the high dose lycopene treated groups, lycopene increased
the toxic effect of NaF synergistically. As a result, it was concluded that administration of lycopene on
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NaF applied renal cell line exhibited different effects based on the dose and time. Keywords: NaF,
Lycopene, Cell Culture, MTT, Kidney.

KEYWORDS
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NaF, Lycopene, Cell Culture, MTT, Kidney


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Corresponding Author: SEDAT ETN, sedatcetin81@hotmail.com


1
YZNC YIL NVERSTES VETERNER FAKLTES, BYOKMYA ANABLM DALI
2
MULA SITKI KOMAN NVERSTES
3
YZNC YIL NVERSTES TIP FAKLTES, TIBB BYOLOJ ANABLM DALI
4
YZNC YIL NVERSTES ZALP MESLEK YKSEKOKULU

1721
Poster Session 13
Submission ID: 1726

SOME PROPERTIES OF ESSENTIAL OIL ADDED TURKISH


TRADITIONAL BEVERAGE BOZA

1 1 1 1
ELF AKIR , HATCE EBRAR KIRTIL , YRD. DO .DR M.ZEK DURAK , PORF. DR. MUHAMMET ARICI

ABSTRACT
Aromatic essential oils are used as a natural preservative due to their antibacterial properties,
taste, flavoras well as increasing their awareness on health benefits . Forth is purpose, we aim to bring
new flavors and functional properties to boza without changing the original appearance of this
traditional beverage. Raw boza was produced by using 1: 1 ratio of bulgur and rice. Boza was
fermented by adding 2% traditionally fermented boza purchased from a local producer. At the end of
the first fermentation period, ginger (Zingiber officinale), sage (Salviaofficinalis L.),fennel
(Foeniculum vulgare) essential oils obtained by hydrodistillation method from Clavenger were added.
Ginger essential oil is used 500 ppm due to its sharp smell, fennel sage essential oils are used 600 ppm
based on initial sensory tests. Physicochemical, microbiological and sensory characteristics of the
study were investigated. Boza samples were stored 4 C for 5 days. The pH values during store of
standard (control) boza was found to be 3.64- 3.50; boza with sage essential oil was 3.55-3.48; boza
with fennel essential oil was 3.53-3.49; boza with ginger essential oil was 3.54-3.48. Standart (control)
boza has been seen greatest decrease. Titratable acidity (TTA) were at standard boza 0.3%-0.47%;
boza with sage essential oil 0.3%-0.37% ; boza with fennel essential oil 0.32%-0.38% while boza with
ginner essential oil was found between 0.34%-0.39%. Alcohol content was found to be in standard
boza (0.7% -0.9%); boza with sage essential essential oil (0.62% -0.66%); boza with fennel essential
oil (0.66% -0.70%) and boza with ginger essential oil (0.63% -67%), respectively. The highest alcohol
content was found in standard boza. Brix values in bozas (14.8% -13.7%) were not significantly
different. At the same time, there was no significant difference between the color of standard boza and
boza with essential oils in color parameters. According to microbiological analysis, total number of
mesophyllaerob bacteria (MAB) were found in standard boza (7,84-8,74 log CFU/g), boza with fennel
essential oil (7,91-8,08 log CFU/g), boza with sage essential oil (7,82-7,9 log CFU/g) and boza with
ginger essential oil (7,77-7,72 log CFU/g), a higher number of bacteria were found in standart
(control) boza and boza with fennel essential oil . During storage (5 days), number of lactic acid
bacteria were reduced in all samples and the highest rate was found in boza with ginger essential oil
(7,81-7,55 log CFU/ g). Total yeast was only increased in standard boza (6,5-6,6 log CFU/g), but was
reduced in boza with fennel essential oil (5,9-5,67 log CFU/g), boza with sage essential oil (6,4-6,02
log CFU/g) and boza with ginger essential oil. In addition, antimicrobial activities of these essential
oils added boza samples were determined on 8 pathogenic bacteria and 2 yeasts. The antibacterial
effects (inhibition zone diameter) of sage essential oil, fennel essential oil, ginger essential oil were
fixed on Candidaalbicans ATCC 10251 (15,5 mm) and Escherichiecoli ATCC 25922 (17,8 mm);
Staphylococcusaureus ATCC 25923 (11,3 mm) and Listeriamonocytogenes ATCC 13932 (11,8 mm);
Listeriamonocytogenes ATCC 13932 (12,3 mm) and Bacillussubtilis(14,5 mm), respectively. Total
antioxidant capacities of bozas withess ential oils were similar to each other. Only the sage essential

Corresponding Author: ELF AKIR, cakir.elif2014@gmail.com


1
YILDIZ TEKNK NVERSTES GIDA MHENDSL

1722
oil added boza was found to be higher(37.58%) than the others. As for sensory analysis, there was no
difference on appearence and consistency among boza samples. Interms of taste, standard boza and
boza with ginger essential oil were the best. As a result, boza with ginger essential oil is considered as
an alternative and healtier option for boza.

KEYWORDS
boza,essential oil ,sage,ginger,fenel

1723
Poster Session 13
Submission ID: 1727

UTILIZATION OF EST RESOURCES OF PERILLA FRUTESCENS L.


FOR SSR MARKERS DEVELOPMENT

1 1 1
AYE GL NCE , MEHMET KARACA , ADNAN AYDIN

ABSTRACT
Perilla also known as beefsteak mint (Perilla frutescens (L.)) is a self-compatible annual herb
in the family Lamiaceae. The beefsteak mint plant has a long history of use in traditional folk
medicine and is an economical crop in the medicinal herb plant. Perilla has several important
pharmaceutical, anti-allergic, and antioxidant functions. Perilla leaf composes of a number of chemical
variants of the volatile essential oils such as perillaldehyde, elsholtziaketone, perilla ketone, perillene,
phenylpropanoids, and piperitenone. Its seeds can be processed into foods and nutritional edible oils.
The use of DNA markers in perilla breeding for development of promising cultivar and conservation
studies is very limited. DNA markers are the best method for the selection of plants with desirable
characteristics. DNA markers have been extensively used in plant improvement studies. Polymerase
chain reaction (PCR) based genotyping of molecular markers such as random amplified polymorphic
DNA (RAPD) markers, amplified fragment length polymorphisms (AFLPs), and simple sequence
repeats (SSRs, also known as microsatellites) have provided useful information regarding genetic
diversity and genetic relationships in many crops. SSRs are considered the marker of choice in many
applications because; (i) they are easy to develop since they are based on polymerase chain reaction
(PCR); (ii) development of SSR markers does not require a high amount and high quality of genomic
DNA; (iii) SSR markers can differentiate homozygote samples from heterozygote ones since they are
co-dominant markers, and (iv) SSRs are highly polymorphic since they are often multiallelic and
hypervariable. However, there is no report on the use of SSR markers for perilla due to a scarcity of
primer pair. Expressed sequence tags (ESTs) are fragments of expressed genes occurring from single-
pass sequencing of cDNA libraries. ESTs have been utilized for many purposes including the
development of SSR markers. In the study, a total of 5349 Perilla frutescens (L.) expressed sequence
tags (ESTs) from National Center for Biotechnology Information (NCBI) were downloaded and EST-
SSRs were identified using the Tandem Repeats Analyzer 1.5 (TRA 1.5) program. SSRs were
searched using the following criteria: 9 repeats for di-nucleotides, 7 repeats for tri-nucleotides, 6
repeats for tetra-nucleotides and 5 repeats for penta-nucleotides and 4 repeats for hexa-nucleotides.
Mining analyses indicated that the most common SSRs were di-nucleotide repeats (6.65%) followed
with tri-nucleotide repeats (3.01%). Among the tetra- (0.2%), penta- (0.3%) and hexa-nucleotide
(1.2%) repeats, di-nucleotide repeats were abundant in SSRs of perilla. EST-SSR primer pairs flanking
the microsatellite domains were designed using PRIMER3 software. SSR primer pairs were designed
with several criteria such minimum/maximum as length of expected product, GC content, melting
temperature etc. Although results revealed that a total of 500 EST-SSR primer pairs could be
designed, for the preliminary studies 17 EST-SSR primer pairs were designed. Annealing temperatures
of the primer pairs are set to 55-56 C (Tm 59-60 C) and expected to amplify markers ranging from 211

Corresponding Author: ADNAN AYDIN, aydina@akdeniz.edu.tr


1
AKDENZ NVERSTES

1724
bp to 300 bp in size. These SSR primer pairs could be useful in genetic characterization and breeding
studies of beefsteak mint.

KEYWORDS
Beefsteak mint, DNA markers, ESTs, microsatellites, perilla, SSR

1725
Poster Session 13
Submission ID: 1728

AN INSIGHT INTO MICROPROPAGATION OF WATER HYSSOP


(BACOPA MONNIERI L.)-AN IMPORTANT MEDICINAL AQUATIC
PLANT

1 ! 1 1 1
FATMA NUR DOANAY , BURAK KAHVECI , ECE NUR KORKMAZ , MERT ELIKEL , MUHAMMAD AASIM ,
1
MEHMET KARATA

ABSTRACT
Bacopa monnieri L. (Water hyssop or brahmi) is one of the important medicinal aquatic/semi-
aquatic plant of traditional Indian medicinal system. It contains important bio-active compounds like
Bacosides that are used as commercial memory enhancer tonic. Number of Brahmi based registered
drugs available are used for curing chronic diseases and disorders like cancer, asthma, mental illness,
Alzheimers disease, anxiety, respiratory ailments, and stomach ulcers. Due to its high demand, it is
collected from wild which make this plant threatened to extinction. Propagation through seed is
limited due to low seed viability and availability. Propagation through tissue culture techniques
provides alternative way of production and conservation of this important aquatic plant. In recent
years, large number of studies has been conducted to develop efficient and reproduce-able in vitro
regeneration with objective to conserve and produce plants for continuous availability of secondary
metabolites it contain. This study present an overview of in vitro regeneration of water hyssop through
axillary or adventitious shoot proliferation using different explants, plant growth regulators and culture
conditions like light followed by rooting and adaptation techniques.

KEYWORDS
Aquatic, In vitro, medicinal micropropagation, Water Hyssop

Corresponding Author: MUHAMMAD AASM, mshazim@gmail.com


1
DEPARTMENT OF BOTECHNOLOGY, FACULTY OF SCENCE, NECMETTN ERBAKAN UNVERSTYY, 42060
KONYA, TURKEY

1726
Poster Session 13
Submission ID: 1729

THE IMPORTANCE OF OLIVE AND OLIVE INDUSTRY BY-


PRODUCTS IN ANIMAL FEEDING

1 1 2
HSEYIN AYAN , AHMET AHN , GRAY ERENER

D
ABSTRACT
In recent years, the importance of natural products and the trend towards nature and the

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importance of medical and aromatic plants are increasing day by day. Our country, which has rich
flora due to its geographical location, is also diversified in terms of medical and aromatic plants. Olive
tree (Olea europaea) leaves have been widely used in traditional remedies in European and
Mediterranean countries. Olive leaves, branches, olive cake and waste water are main olive by-
products during the progress of harvesting to olive oil production. The use of agricultural by-products
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in animal nutrition is as old as the domestication of animals. Among the main advantages of using by-
products in animal feeding are the reduced dependence of animals on cereals consumed by humans
and the reduction of administration costs of waste products. Olive and olive by-product contain many
potentially bioactive compounds that may have antioxidant, antimicrobial, antihypertensive,
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antiatherogenic, anti-inflammatory, hypoglycemic, and hypocholesterolemic properties. The most
important of these bioactive compounds are oleuropein and hydroxytyrosol. In this review, it will be
addressed to the possibilities of using by-products from olive and olive oil production in animal
feeding as an alternative and to the results of several related studies.
PR

KEYWORDS
Olive, Animal feeding, Olive by-product, Oleuropein
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N

Corresponding Author: HSEYN AYAN, huseyin.cayan@ahievran.edu.tr


1
AH EVRAN NVERSTES ZRAAT FAKLTES ZOOTEKN BLM, KIREHR
2
ONDOKUZ MAYIS NVERSTES ZRAAT FAKLTES ZOOTEKN BLM, SAMSUN

1727
Poster Session 13
Submission ID: 1732

EVALUATION OF THE FATTY ACID COMPOSITIONS OF 14


PISTACIA VERA FRUITS WITH CHEMOMETRIC APPROACH

1 1 1 1 1
HSEYN TRKOLU , BHTER AHN , ZGE TOKUL LMEZ , ERHAN KAPLANER , MEHMET ZTRK ,
1
MEHMET EMN DURU

ABSTRACT
Pistacia genus which is a member of Anacardiaceaehas a shelled edible fruit. This fruit is
called as am fst in some regions. Pistacia genus could be raised easily such places like barrened
and limed land where other plants can not be cultivated. This genus is widely distributed from the
Middle Asia to Anatolia. Iran, Turkey and U.S.A. shared first 3 position in the production of Pistacia.
According to some anonymous data %33 of the production is made in Urfa. Pistacia consist more than
%50 of oil and more than %20 of protein. Omega-3, omega-6 and highly unstaturated fatty acids are
the major components of pistacio oil along with B-3, B-12,vitamine-A, provitamine-B5 (Panthenol)
and B-1 (thio-vitamine) (1). In this study fatty acids of 14 pistacia vera from the different locations in
Urfa were analyzed by GC-MS. In consideration of the fatty acid concentrations Principle Component
Analysis (PCA) and Hierarchical Clustering Analysis (HCA) were applied using Minitab software.
Relations between the samples according to their locations and other properties were revealed.
Referanslar (1) Satil, F.; Azcan, N.; Baser, K. H. C. Fatty acid composition of pistachio nuts in
Turkey. Chem. Nat. Compd.2003, 39 (4), 322324.

KEYWORDS
Pistacia vera, GC-MS, PCA, HCA, chemometry

Corresponding Author: BHTER AHN, bihtershn89@gmail.com


1
MULA SITKI KOMAN NVERSTES

1728
Poster Session 13
Submission ID: 1733

DIETARY USAGE OF FERULA ELEAOCHYTRIS POWDER IN


ANIMAL NUTRITION

1 2 1 1 1 1
GKHAN FILIK , HAYRETTIN AYIROLU , AYE GL FILIK , HSEYIN AYAN , SA COKUN , AHMET AHIN

D
ABSTRACT
Ferula is a genus of about 170 species of flowering plants in the familiy Apiaceae, native to

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the Mediterranan region to East to Central Asia, mostly growing in arid climates. They are herbaceous
perennial plants and present dominantly Kelda of Yalada District of Hatay Province, as called
akrin Turkey. Animals, especially pastured goats, consume its leaves, enhancing reproductive
performance in those animals, as known in folk literature. The root powder of this plant was used in
small ruminant and poultry experiments. According to findings obtained from those studies the
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following outcomes; (1) having no harmfull effect on animal health, (2) showing hypoglicemic and
hypocholesteromic effects in layer hens (3) improving carcass parameters in broiler chicks, (4)
changing secondarily sex charactheristics in broiler chicks and quails, (5) and having eostrogenic
effect in small ruminants. To conclude, ferula root powder has been needed be studied in detailed
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manner to produce new feed additives in animal nutrition.

KEYWORDS
PR

Ferula eleaochytris, feeding, farm animals, feed additive


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Corresponding Author: GKHAN FLK, gfilik@ahievran.edu.tr


1
AH EVRAN UNVERSTY AGRCULTURE FACULTY, DEPT. OF AGRCULTURAL BOTECHNOLOGY,
KIREHR
2
AH EVRAN UNVERSTY AGRCULTURE FACULTY DEPT. OF ANMAL SCENCE, KIREHR

1729
Poster Session 13
Submission ID: 1735

A HEALING PLANT "MENENGIC"

1 1 1
MEHMET KTEN , HALIL EKICI , MUSTAFA SATOUF

ABSTRACT
Terebinth tree (Pistacia terebinthus) is a member of Anacardiaceae family, native to
Mediterranean, evergreen and grows ecologically without plantation in the mountainous and rural
areas of Mediterranean, South East and Central Anatolian regions of Turkey. It is also known as
itlembik, tlk, itemik and bttm; named differently in different regions. Terebinth fruit is
consumed as fresh or dried, as a coffee and used in traditional bread making in South East and
southern regions of Turkey. In the same regions, terebinth paste is mixed with different spices and
seasonings in order to prepare the mixture known as (kahvaltlk zahter) breakfast zahter. Due to
rich tannin and resin content of terebinth; it is considered as a medicinal plant since ancient times.
Ripe terebinth fruit is rich in protein, oil, dietary fiber, unsaturated fatty acid and mineral content.
Terebinths fatty acid composition determined with gas chromatography is %52.3 oleic acid, %21.3
palmitic acid ve %19.7 linoleic acid. Sodium and phsophorus content is higher than the olive and
banana fruits. Also it has been determined that terebinth has a higher potassium, phosphorus, calcium
and iron content than potato. Due to high protein, oleic acid and linoleic acid content, terebinth is a
healthy dietary element. In this study, Terebinth plant which has a strong potential as a functional food
in terms of its components has been examined in the context of its all features.

KEYWORDS
Menengic, Citlembik, Menengic fruit, Pistacia terebinthus

Corresponding Author: MEHMET KTEN, mehmetkoten@gmail.com


1
KLS 7 ARALIK NVERSTES

1730
Poster Session 13
Submission ID: 1736

ANTIOXIDANT ACTIVITY OF POLYSACCAHARIDE EXTRACTS OF


TRICHOLOMA ANATOLICUM

1 1 1
ERHAN KAPLANER , MEHMET HSEYIN SNGE , MEHMET ZTRK

ABSTRACT
Because having important nutrients (high protein content, rich fatty acid constituents) along
with phenolics, terpenes, steroids and polysaccharides of medicinal mushrooms have been studied
very frequently. Polysaccharide containing mushrooms show anti-inflammatory, cytotoxic and anti-
viral properties and immunomodulatory activities1. Tricholoma anatolicum which is naturally growing
in Fethiye-Mula. It is the commercial importance because of the similarity to that of Tricholoma
matsutake, and was selected for this study. Mushroom samples collected from Fethiye-Mugla and
brought to laboratory. All samples were air-dried under shadow. After grinding the dried mushrooms
organic solvent extraction were applied with the increasing polarity (petroleum ether, acetone,
methanol, successively). After taking the methanol extract the residue was used for hot water
extraction. Ethanol was added to supernatant part (1:3 v/v) to precipitate the polysaccharides2, this
part was coded HWEP and the remaining part was coded HWES. Antioxidant activities of these
extracts were determined according to DPPH, ABTS+, -carotene-linoleic acid, metal chelating and
CUPRAC assays. HWES showed 81.9% lipid-peroxidation inhibitory activity at a concentration of 0.8
mg/mL while HWEP showed 78.0%. Both extracts were inactive against DPPH radical but HWES
showed 82.8% ABTS+ radical scavenging activity. HWES had more ability for metal-chelating
activity when compared with that of HWEP. However, in CUPRAC assay HWEP exhibited better
activity Acknowledgements: This study is supported by TUBITAK-SBAG with the Project number of
113R012. References (1) Zhu, H.; Sheng, K.; Yan, E.; Qiao, J.; Lv, F. Extraction, purification and
antibacterial activities of a polysaccharide from spent mushroom substrate. Int. J. Biol. Macromol.
2012, 50 (3), 840843. (2) Villares, A.; Garca-Lafuente, A.; Guillamn, E.; Mateo-Vivaracho, L.
Separation and characterization of the structural features of macromolecular carbohydrates from wild
edible mushrooms. Bioact. Carbohydrates Diet. Fibre 2013, 2 (1), 1521.

KEYWORDS
Tricholoma anatolicum, polisakkarides, antioxidant activity

Corresponding Author: ERHAN KAPLANER, kaplaner.erhan@gmail.com


1
MULA SIKTI KOMAN NVERSTES, FEN FAKLTES

1731
Poster Session 13
Submission ID: 1737

HEALING EFFECTS OF BALNEOTHERAPY ON CLINICAL


SYMPTOMS IN DIFFERENT PATIENTS: SURVEY-BASED STUDY

1 2 3 4 5
AHMET CELIKKAYA , BULENT ELTOK , SMAIL ASLAN , BEKIR AKICI , OZGUL M. ELITOK

D
ABSTRACT
Aims. The aim of this survey-based study was investigated in the clinically healing effects of

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traditional balneotherapy methods in human (in future also in animals) suffured from different
diseases in thermal areas Afyonkarahisar and Gazlgol-Yaylabagi. Materials and Methods. The people
had always flocked to the sources of natural mineral spring water for healing (balneotherapy). In the
present study, people with different diseases had received balneo- therapy as mud bath, hot water bath,
drinking and bathing especially by themself and according to their own knowledge. Results. The
EN
results obtained from this study show that balne thearpy (drinking and bathing) was provided some
clinical healing in most patient, but some unwilling effects of therapy had occured due to their
unciously use the therapy. Discussion. Although some unwilling effects of traditional balneotherpy use
were seen in some patient, the results of study shown that received balneotehary methods (drinking
ES
and bathing especially) were effective on clinical healing of some diseases in human beings. In future,
our other aim will be using in animal diseases.

KEYWORDS
PR

Afyonkarahisar, balneotherapy, SPA, traditional methods


T
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Corresponding Author: BULENT ELTOK, elitok1969@hotmail.com


1
MEDCAL DOCTOR OF EMERGENCY SERVCE, AFYONKARAHSAR GOVERNMENT HOSPTAL, 03200-
AFYONKARAHSAR/TURKEY
2
CORRESPONDNG AND PRESENTER AUTHOR, ASSOC. PROF. DR., CHARMAN OF WHOLE
BALNEOTHERAPY AND NATURAL TREATMENTS ASSOCATON/ AFYON KOCATEPE UNVERSTY,
FACULTY OF VET. MED., DEPT. INTERNAL MED. 03200-AFYONKARAHSAR/TURKEY
3
PHARMACST., YEDTEPE UNVERSTY, COSMETC RESEARCH AND PRODUCE CENTER (YUKOZ),
ATAEHR, 34100-STANBUL/TURKEY
4
CHEMST., YEDTEPE UNVERSTY, COSMETC RESEARCH AND PRODUCE CENTER (YUKOZ), ATAEHR,
34100-STANBUL/TURKEY
5
5DR. VET. MED., AFYONKARAHSAR RURAL DEVELOPMENT AGENCY, 03200-AFYONKARAHSAR/TURKEY

1732
Poster Session 13
Submission ID: 1739

DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF


HAPLOPHYLLUM BUXBAUMII PLANT IN DIFFERENT SOLVENTS

1 ! ! ! 1 1
SMAIL KOYUNCU , ADEM NECP , BRAHIM BEKTA , MESUT IIK , ABDURRAHMAN AKDA , MER G

ABSTRACT
Most of the potential damage from oxygen is occured by the formation of reactive oxygen
species (ROS). Uncontrolled produced ROS and unbalanced antioxidant mechanisms is the cause of
many diseases [1]. Natural antioxidants are very important in the regulation of unbalanced antioxidant
mechanism. The antioxidant effect of the phenolic compounds contained in the plants protects the
cells from potential damage [2]. This study was designed to determine the phenolic and flavonoid
species and the antioxidant activity of methanol, butanol, hexane, dichloromethane and water extracts
in haplophyllum buxbaumii plant. The total phenolic and flavonoid compounds in the plant were
determined using Folin-Ciocalteu reactivity. The antioxidant activity of plant extracts was determined
using the DPPH and ABTS which method of free radical scavenging activity test and the FRAP and
CUPRAC which metal reduction capacity tests. BTH, Trolox, Ascorbic acid and Gallic acid were used
as standard for antioxidant activity comparison. In this study, antioxidant activity of butanol extract
was found to be more effective than the other extracts in FRAP and CUPRAC methods. It was
observed that all extracts showed free radical scavenging activity in similar proportions in the DPPH
test, the butanol extract in the ABTS test showed more activity than the other extracts and standards.

KEYWORDS
Haplophyllum buxbaumii, Antioxidant, Reactive oxygen species

Corresponding Author: ADEM NECP, ademnecip@harran.edu.tr


1
HARRAN NVERSTES

1733
Poster Session 13
Submission ID: 1740

A SYSTEMATIC REVIEW OF FIBER FOODS USED FOR


CONSTIPATION REMOVAL

1 2
FILIZ HISAR (DO. DR.) , KEMAL MACIT HISAR (YRD. DO. DR.)

ABSTRACT

D
Introduction: Constipation is a health problem affecting 20% of the world population. This
condition is discomfort that affects the quality of life of the individual, causes colon cancer, and

TE
increases in severity with time. Constipation is an important preventable health problem that is
common in women, children and the elderly. There are some risk factors for constipation, such as
physical inactivity, gender, age, low level of education, socio-economic status, sexual abuse, presence
of depressive symptoms, nonsteroidal antiinflammatory drugs, some diseases. According to the studies

EN
done, in women, in children, there is more frequent constipation in the elderly. There are many
different treatment methods in constipation. As first-line treatment, lifestyle changes, fluid intake or
exercise are recommended. However, the efficacy of these applications is limited. Laxatives are
commonly used in constipation therapy. However, the use of these drugs often causes some adverse
effects. Fibrous nutrients are recommended for the prevention and treatment of constipation. Taking
ES
fibrous foods in the diet is a positive approach to healthy eating. Increased dietary fiber intake in
children and adults is recommended for the prevention and treatment of insomnia. It is stated that
Ayaz and Hisar (2014), "Efficiency in the elimination of constipation of education and counseling
services given to women", prevented the constipation with the intake of fiber foods in the diet. Studies
in the form of a systematic review of the relationship between intake of fibrous nutrients and
PR

constipation in diets are not sufficient. For this reason this work is planned. Purpose of the study: This
study aims to examine the systematic compilation of plant materials applied to eliminate constipation
in the world and in our country. Method: The survey was conducted between 15-20 March 2017.
Using electronic search engines, we obtained 360 articles and reached 49 articles by using the fibrous
nutritional statement in Wiley-blackwell, Web of Science, Scopus, Science Direct Journals, Pubmed,
T

Medline, and Cinahl plus full text databases. In the Constitution statement, 87 articles were reached,
64 articles have been reached with the expression and 2 articles have been reached with systematic
O

compilation statement. In addition, 2 articles have been reached with the expression of herbal
applications. The other side also scanned the google search engine (45 English, 8 Turkish articles
reached). A total of 249 articles have been reached. It has been suggested to use fibrous nutrients
N

applied to remove constipation in 11 articles. Conclusions: It has been found that 92% of the studies
investigating the use of fibrous nutrients for removal of constipation are inadequate for fibrous nutrient
uptake. It is stated that the number of feces and the amount of fiber increases with the consumption of
fiber foods.

KEYWORDS
fibrous nutrients, constipation, herbal applications, public health, systematic review

Corresponding Author: KEMAL MACT HSAR (YRD. DO. DR.), kmhisar@hotmail.com


1
N.E. NVERSTES SALIK BLMLER FAKLTES
2
S.. TIP FAK. HALK SALII AD.

1734
Poster Session 13
Submission ID: 1741

GINSENG AND ITS HEALTH DEVELOPER FUNCTIONS

1 1 2
SERAY AKALIN , MERVE ZDEMIR , FERAY GENER

ABSTRACT
Ginseng is one of the plants used for treatment of many diseases for many years. Its use has
spread all over the world from East Asian countries like China, Korea and Japan. Ginseng has been
shown in many studies to have positive effects in many cases such as neurological diseases, immun
system disorders, diabetes, cancer, cardiovascular diseases. Panax ginseng is the most valuable and
important among the ginseng species. It has been used in East Asian medicine for centuries. The habit
of using plants for medical purposes and healthy nutrition in Asia has also been adopted by western
societies in recent years. Thus, P.ginseng has become available all over the world. Ginsenosides are
main bioactive compuonds of P.ginseng. More than 50 ginsenosides have been isolated from P.
Ginseng. Ginsenosides are a class of saponins with dammarane triterpenoid structure. Many human
and animal studies have been conducted to investigate the effects of ginseng on health. Based on the
results of these studies, it has been shown that ginsenosides and its different fractions have positive
effects on the regulation of the immune response. It is also concluded that ginsenosides have important
roles in tumor cell cytotoxicity against cancer development, differentiation of tumor cells,
development of inflammation suppressor cells, prevention of metastasis, prevention of angiogenesis.
Positive effects of ginsenosides on regulation of blood glucose level and treatment of diabetes as well
as immune response and anticarcinogenic effects have been observed. It affects energy homeostasis by
regulating insulin secretion and glucose transport as well as affecting peroxisome proliferator-
activated receptor (PPAR-) and AMPK function, these are two important proteins that are effective in
adipocyte differentiation. One of the known effects of ginseng is the positive effects on the central
nervous system. The neurotrophic effect on memory and learning is protective against neuronal
damage. Ginseng has recently been a highly studied plant and different effects of ginsenosides on
health are being explored further.

KEYWORDS
Ginseng, ginsenosides, functional foods, P. ginseng

Corresponding Author: SERAY AKALIN, serayakalin@hacettepe.edu.tr


1
HACETTEPE NVERSTES
2
GAZ NVERSTES

1735
Poster Session 13
Submission ID: 1742

THYMOL ATTENUATES INDOMETHACIN-INDUCED


MACROMOLECULAR DAMAGES IN SMALL INTESTINE

1 2 2 1 1
FATIME GEYIKOGLU , NIHAL SIMSEK OZEK , FERHUNDE AYSIN , KUBRA KOC , SALIM CERIG

ABSTRACT
THYMOL ATTENUATES INDOMETHACIN-INDUCED MACROMOLECULAR
DAMAGES IN SMALL INTESTINE Fatime Geyikoglu1, Nihal Simsek Ozek*1,2, Ferhunde
Aysin1,2, Kubra Koc1, Salim Cerig1 *Corresponding author: Nihal Simsek Ozek, e-mail:
nihal.ozek@atauni.edu.tr phone number: + 90 0 442 231 1649 1Department of Biology, Faculty of
Science, Ataturk University, Erzurum, TURKEY 2East Anatolian High Technology Research and
Application Center (DAYTAM), Atatrk University, Erzurum, TURKEY Indometacin, a nonsteroidal
anti-inflammatory drug (NSAID), is commonly and efficiently used to reduce fever, pain and swelling
from inflammation. In spite of its therapeutic potentials, it has adverse effects on the gastrointestinal
system and the occurrence of these effects are associated with oxidative stress. It has been known that
this stress causes macromolecular damages in biological systems and these effects can be decreased by
the use of bioactive components of plants such as thymol etc. Although, the healing roles of several
plant based products such as curcumin in the indomethacin induced damages of small intestine have
been indicated, the attenuating role of thymol in these damages in terms of the structure and function
of intestine macromolecules has not been demonstrated yet. The current study was established to
elucidate these effects by Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR)
Spectroscopy together with chemometric analysis approach. Rats received distilled water (control),
indomethacin (25 mg/kg), indomethacin+ rantidine (50 mg/kg ran) and indomethacin+thymol
combinations (100, 250 and 500 mg/kg Thymol) orally by intragastric gavage. Firstly, the spectra of
small intestine samples were collected and then detailed spectral analyses including frequency, band
area and bandwidth of spectral bands were performed to determine the structural and functional
alterations in the tissue macromolecules. To discriminate the studied groups based on these alterations,
hierarchical cluster (HCA) and principal component analysis (PCA) were applied to their spectra. The
spectral results demonstrated that indomethacin caused significant damages in the unsaturated,
saturated lipid, protein, nucleic acid amounts and also membrane fluidity with respect to the control
and these damages may be related with the indomethacin-induced oxidative stress. On the other hand,
especially 250 mg/kg dose of thymol administration ameliorated these damages with regards to the
indomethacin group. The chemometric analysis indicated that the thymol treated groups were
successfully discriminated from indomethacin group. The results of the current study suggest that
thymol has the curative potential of the adverse effects of indomethacin in small intestine. Keywords:
Indomethacin, Small intestine injury, Thymol, FTIR spectroscopy, Chemometric analysis.

Corresponding Author: NHAL SMSEK OZEK, nihal.ozek@atauni.edu.tr


1
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, ATATURK UNVERSTY, ERZURUM, TURKEY
2
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, ATATURK UNVERSTY, ERZURUM, TURKEY, 2EAST
ANATOLAN HGH TECHNOLOGY RESEARCH AND APPLCATON CENTER (DAYTAM), ATATRK
UNVERSTY, ERZURUM, TURKEY

1736
KEYWORDS
Indomethacin, Small intestine injury, Thymol, FTIR spectroscopy, Chemometric analysis.

1737
Poster Session 13
Submission ID: 1743

EFFECTS OF INFRARED RADIATION DRYING PROCESS ON


ASCORBIC ACID CONTENT AND ANTIOXIDANT ACTIVITY OF
DILL LEAVES

1 2 3 3 3
FILIZ IER , MUTLU EVIK , DAMLA BAYANA , DENIZ DNER , MER FARUK OKGEZME

ABSTRACT
Dill (Anethum graveolens l.), is a biennial or annual herb of the parsley family (Apiaceae or
Umbelliferae). Because of its rich antioxidant activity, ascorbic acid and total carotenoid content, dill
is utilized as pharmaceutic plant. However, storage of fresh dill is very problematic due to its high
moisture content, thus drying of it is one of the common preservation methods. In case of conventional
air circulation drying systems heat is transferred to product from air by convection, therefore drying
process takes long times and results in undesired changes in the product. In recent years, novel drying
methods shortening process time have been studied. Infrared drying, which is one of these novel
methods, is suitable for drying of the materials having high surface area and drying in the form of thin
layer. In infrared drying, drying time is shortened and loss of the desired components is reduced due to
its high heat transfer rate. In present study, dill leaves in the form of thin layer were dried in infrared
drier at two different radiation power (85 and 102 W) from initial moisture content of 822 % to the
final moisture content of %81 moisture content. Changes of drying times, antioxidant activity and
ascorbic acid values depending on infrared power were determined. Drying times for low and high
power levels were determined as 840 s and 620 s, respectively. It was determined that antioxidant
activity values increased as the radiation power increased. The loss in ascorbic acid contents of dried
samples were determined as 12% and 75% for low and high power, respectively. It was concluded that
the loss of ascorbic acid content during infrared drying was more affected from radiation power than
drying time. It was determined that as the radiation power increased by 20% for infrared drying of dill
leaves having medical and aromatic value, its drying time was shortened by 27% and its antioxidant
capacity increased while the degradation of ascorbic acid content increased. It was recommended that
the optimum process parameters for infrared radiation drying of dill leaves should be determined by
optimization studies taking into account of changes in quality attributes in addition to drying times and
performance characteristics.

KEYWORDS
dehydration, quality, power, medical, aromatic, seasoning

Corresponding Author: MER FARUK OKGEZME, ofarukcokgezme@gmail.com


1
EGE NVERSTES, GIDA MHENDSL BLM
2
MUNZUR NVERSTES, GIDA MHENDSL BLM
3
EGE NVERSTES, FEN BLMLER ENSTTS, GIDA MHENDSL ANABLM DALI

1738
Poster Session 13
Submission ID: 1745

EVALUATION OF CYTOTOXIC EFFECTS OF RHEUM RIBES (ROOT


AND STEM) EXTRACTS ON HCT 116 COLORECTAL CANCER
CELLS

1 1 1 2
LKNUR INAR , SMEYRA ETINKAYA , HATICE GL DURSUN , PEK SNTAR

ABSTRACT
Colorectal cancer (CRC) is one of the most frequent type of cancer that has rapidly increasing
incidence, and shorter duration of survival. Due to serious side effects and chemo resistance of most
chemotherapeutic drugs used in the treatment of CRC, it becomes necessary to develop new cytotoxic
agents. It is thought that, secure extracts of edible plants which contain structurally different molecules
may prevent the development of multi-factorial diseases as CRC; new efficient strategies are
comprehensively being investigated, including the development of phytopharmaceutics. Our aim in
this study is to evaluate the cytotoxic effects of n-hexane, ethyl acetate, methanol extracts from stem
and root of Rheum ribes, by applying them to the HCT 116 colorectal cancer cells. The samples of the
stem and root of Rheum ribes were dried and powdered, and then extracted with n-hexane, ethyl
acetate and methanol solvents respectively. A total of 6 fractions of this plant were obtained. The
cytotoxic effects of Rheum ribes extracts on HCT-116 cells were assessed using the XTT test. In order
to determine the IC50 dose, plant extracts were applied to the cells at time (24 h, 48 h, 72 h) and dose-
dependent at 50-2000 M. According to the results obtained, the best cytotoxic effect in all fractions
was observed at 48th hour. The IC50 doses according to the fractions were determined as follows: root
n-hexane: 672 M, root ethylacetate: 96.32 M, root methanol: 29.65 M, stem n-hexane: 298.9 M,
stem ethylacetate: 226.6 M, stem methanol: 182.9 M. Among all extracts, root methanol was found
to be the most effective fraction on cytotoxicity.

KEYWORDS
Rheum ribes, cytotoxicity, cancer

Corresponding Author: LKNUR INAR, ilknurcinar@msn.com


1
NECMETTN ERBAKAN NVERSTES, MERAM TIP FAKLTES, TIBB BYOLOJ A.D., KONYA
2
GAZ NVERSTES ECZACILIK FAKLTES FARMAKOGNOZ A.D. ANKARA

1739
Poster Session 13
Submission ID: 1746

EFFECTS OF TIMOKINONE APPLICATION IN THE RENAL CELL


LINE

1 1
VEYSEL YKSEK , SEMIHA DEDE

D
ABSTRACT
EFFECTS OF TIMOKINONE APPLICATION IN THE RENAL CELL LINE This study was

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planned to investigate the cytotoxic effects of thymokinon (TQ) which is a major component of
Nigella sativa plant, which is widely used in various forms and reasons, in NRK-52E rat kidney cell
line at various doses and durations. NRK-52E rat kidney cells were grown by regular passages in vitro
conditions. TQ solutions prepared at different doses were added per well and MTT viability test was
performed at 6, 12 and 24 hours. As a result, it was revealed that TQ administration NRK-52E is
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dependent on different dosages and duration; Low doses have permanently increased cell proliferation
and cytotoxic activity has been consistently elevated as TQ doses are increased. Based on these
results, it was concluded that the reliability of TQ administration in relation to dose and time on the
kidneys should be evaluated in further studies.
ES

KEYWORDS
Thymokinon, Cell culture, MTT, Cytotoxicity, Kidney
PR
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N

Corresponding Author: VEYSEL YKSEK, veyselyuksek@yyu.edu.tr


1
YZNC YIL NVERSTES

1740
Poster Session 13
Submission ID: 1751

AMELIORATIVE EFFFECTS OF CARVACROL ON EXPERIMENTAL


ACUTE PANCREATITIS

1 2 2 2 2
NIHAL SIMSEK OZEK , FATIME GEYIKOGLU , MIRKHALIL HOSSEINIGOUZDAGANI , KUBRA KOC , SALIM CERIG ,
3
GLSAH YILDIZ DENIZ

ABSTRACT
AMELIORATIVE EFFFECTS OF CARVACROL ON EXPERIMENTAL ACUTE
PANCREATITIS Nihal Simsek Ozek1,2 Fatime Geyikoglu1, Mirkhalil Hosseinigouzdagani1, Kubra
Koc1, Salim Cerig1, Glsah Yildiz Deniz3 1 Department of Biology, Faculty of Science, Ataturk
University, Erzurum, TURKEY 2 East Anatolian High Technology Research and Application Center
(DAYTAM), Atatrk University, Erzurum, TURKEY 3 Health Services, Vocational High School,
Ataturk University, Erzurum, TURKEY Carvacrol, the main compound of thyme, possess
antibacterial, antifungal, antiviral, antitumor anti-oxidant, and anti-inammatory properties. The aim
of this study was to investigate the curative efficacy of carvacrol on acute pancreatitis-induced kidney
injury. With this aim, rats were randomized into control, acute pancreatitis and acute pancreatitis +
carvacrol groups. The animals were given 50, 100 and 200 mg/kg Carvacrol by intraperitonally
injections after 50 g/kg cerulein injection. After experimental procedures, renal tissues were removed
for histological and biochemical investigations. Kidney weight, malondialdehyde (MDA) level, total
antioxidant status (TAS), the blood urea nitrogen (BUN), uric acid (UA) and creatinine (CRE) levels
were measured. Histology of kidney was examined by using three different staining methods: Periodic
acid Schiff, Masson trichrome and Amyloid. According to obtained results, the cerulein treatment
caused significant alterations in the weights of kidneys. However, a significant decrease in the kidney
weights with carvacrol treatment was obtained with respect to the acute pancreatitis group. Moreover,
TAS level was found to be higher in the acute pancreatitis+carvacrol compared to the acute
pancreatitis group. Depending on the increased dose of the carvacrol, the significant decrease in MDA
level was obtained. Also, the BUN, CRE and UA levels of acute pancreatitis and carvacrol groups
were significantly from each other. The marked amelioration of the renal histopathology induced by
carvacrol treatment was observed. Taken together these data showed that 200 mg/kg carvacrol is an
effective therapy against acute pancreatitis-induced renal damages and dysfunction. As conclusion, the
ameliorative effect of carvacrol was associated with its antioxidant properties. Thus, carvacrol was
suggested as natural pharmaceutical agent against pancreatitis-induced multiple organ damages.

KEYWORDS
Acute pancreatitis, Carvacrol, Histopathology, Kidney, TAS.

Corresponding Author: FATME GEYKOGLU, fgeyik@atauni.edu.tr


1
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, ATATURK UNVERSTY, ERZURUM, TURKEY,EAST
ANATOLAN HGH TECHNOLOGY RESEARCH AND APPLCATON CENTER (DAYTAM), ATATRK
UNVERSTY, ERZURUM, TURKEY
2
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, ATATURK UNVERSTY, ERZURUM, TURKEY
3
HEALTH SERVCES, VOCATONAL HGH SCHOOL, ATATURK UNVERSTY, ERZURUM, TURKEY

1741
Poster Session 13
Submission ID: 1752

SEPARATION OF ODOR COMPONENTS FROM HYACINTH


(HYACINTHUS ORIENTALIS) IN DIFFERENT COLORS FOR USE IN
PERFUME CONSTRUCTION AND COMPATIBILITY WITH
SYNTHETIC HYACINTH

1 1
SEDEF AIK , NAZAN DEMR

ABSTRACT
In this study, it is aimed to look at some bioactivity of Hyacinthus orientalis species attracted
to people naturally grown in Mula and its surroundings in February and April, as well as to obtain the
essential oil of the flower and to combine it with the essential oil of other flowers based on the notion
concept And new perfume combinations were selected as the target. The right combination is
considered to be the next step when it becomes a commercial product after patent and trademark work.
Hyacinth is a flower that embellishes nature with its pleasant smell, appearance and colors which are
common in Anatolia. It is known that about 30 subspecies of the hyacinth, an endemic flower species.
Uncultivated onions, local species are still found in Anatolia and are a very popular flower as a part of
our culture. Perfume is a generic name for cosmetic products, derived from fragrant oils, aromatic
mixtures and various chemical substances, which are used to impart fragrance to the human body,
various objects and environments. Briefly a perfume; It is an intuitive and artistic combination that
Chemistry Science has developed from the way of good smell. The white, purple and pink hyacinths
used in this study were selected from the Mula villages and uncastrated local species. They were
diagnosed and worked the day they were collected. The remaining parts were kept at -80 C for other
work. Content analysis by HPLC was performed and the enzyme activities were examined to
investigate the availability of phenolic compounds as well as other cosmetic products in flowers. The
hyacinth flowers are subjected to different processes in order to obtain the essential oils separately.
Extraction with organic solvents, water vapor distillation methods are used in this frame. IR spectra of
different hyacinth hyacinth flowers were compared with the hyacinth essences on the market and
different perfume combinations were studied. The efforts to optimize the successes of different
perfume combinations have been continuing. As a result, the contents of some of the enzyme
activities, essential oil components and phenolic components of hyacinth flowers were determined in
this frame. Cinnamic alcohol, Ethyl 2-methoxybenzoate, 3,7-dimethyl-1, (E) -5 (E) -octatriene-7, (E) -
cinnamic alcohol and ethyl 2-methoxybenzoate. This study also increased the recognition of hyacinth
flowers and provided them as a prestige product. References : [1] Demir N., Ak S., 2017
"Investigation of Some Bioactivity and Odor Components of White Jasmine (Jasminum Officinale)
and Its Use in Perfume Design", Master Thesis. [2] Lehninger, A. L. (2013) Principles of
biochemistry, Worth Publishers Inc., New York, 1152s. [3] Demir N., Demir Y., Kaya E. and Aydn
B., "Cysteine Protease from Primrose (Primula vulgaris)", Asian Journal of Chemistry, 24 (4), 1479-
1482 - 2012. [4] Demir N., Ukaya F., 2015 "Citrus Sinensis (L.) Osbeck (Orange) and Citrus limon

Corresponding Author: SEDEF AIK, demirn@yahoo.com


1
MULA SITKI KOMAN NVERSTES KOZMETK RNLER UYGULAMA VE ARATIRMA MERKEZ
48000, MULA

1742
(L.) Burm.F. Purification of Protease and Peroxidase Enzymes from Lemon (Lemon) Flowers and
Their Use in Industrial Applications ", 44-46s. Doctoral Thesis.

KEYWORDS
Hyacinthus (Hyacinthus orientalis), Odor components, Essential oil components, Phenolic
compounds

1743
Poster Session 14
Submission ID: 581

SHERBET AS A FUNCTIONAL TRADITIONAL DRINK

1 2 3 2
GAMZE TOYDEMIR , TUBA ZDAL , PERIHAN YOLCI MEROLU , SMEYYE BETL KAYA

ABSTRACT
Sherbet is a traditional drink in Turkey which is made from a mixture of a variety of fruits
(tamarind, date, pomegranate, cranberry, red grape, rosehip, locust bean, etc.), spices (black cumin,
cinnamon, ginger, cardamom, clove, etc.), and flowers (rose leaves, carnation, etc.), and further
sweetened with the addition of honey or sugar. Before the appearance of its modern alternatives,
sherbet was a popular drink in the past with its refreshing taste; and it was also used as a curing agent
in medicine due to its health benefits. The main ingredients of sherbet, specifically fruits and spices,
are well-known to be rich sources of antioxidant components that may provide protection against
oxidative damage, and thereby prevent the incidence of several degenerative diseases, such as cancer.
Therefore, sherbet could certainly be considered as a promising functional drink which could regain its
popularity among health-conscious consumers. In this study, we aimed to determine the antioxidant
potentials of three different sherbets, including tamarind, grape, and cranberry sherbets as among the
most preferred sherbet drinks in Turkey. Total phenolic contents (TPC) and total antioxidant capacities
(TAC) of sherbet samples were measured, in triplicates, using the methods of Folin Ciocalteau and
Cupric Reducing Antioxidant Capacity (CUPRAC), respectively. Grape sherbet was found to have the
highest TPC (53 mg gallic acid equivalents (GAE)/ 100 g fresh weight) and TAC (184 mg trolox
equivalent (TE)/ 100 g fresh weight) values among the tested sherbet samples, which was followed by
cranberry sherbet -that had TPC and TAC values of 34 mg GAE/ 100 g fresh weight and 130 mg TE/
100 g fresh weight, respectively- and tamarind sherbet that had TPC and TAC values of 24 mg
GAE/ 100 g fresh weight and 58 mg TE/ 100 g fresh weight, respectively. Current study indicate black
grape, cranberry, and tamarind, which are the main fruit components of the namesake sherbet drinks,
as potent fruit ingredients that could improve the functional properties of these traditional beverages
with their substantial antioxidant potentials. This study is still on progress to reveal the bioactive
properties of other sherbet types by characterizing their major phenolic compounds as well, which will
enable us to highlight the health-related importance of sherbet consumption.

KEYWORDS
sherbet, functional drink, antioxidant capacity, total phenolic content

Corresponding Author: TUBA ZDAL, tugba.ozdal@okan.edu.tr


1
DEPARTMENT OF FOOD ENGNEERNG, FACULTY OF ENGNEERNG, ALANYA ALAADDN KEYKUBAT
UNVERSTY
2
DEPARTMENT OF FOOD ENGNEERNG, FACULTY OF ENGNEERNG, OKAN UNVERSTY
3
DEPARTMENT OF FOOD ENGNEERNG, FACULTY OF AGRCULTURE, ULUDAG UNVERSTY

1744
Poster Session 14
Submission ID: 602

VTS VNFERA L. AND AROMATHERAPY

1 2 3
NESRIN KARACA SANYREK , M. LHAN ODABAIOLU , ATILLA AKIR

ABSTRACT
Aromatherapy is a natural treatment way based on the use of the oils obtained from plants.
The concentrated vegetable energy in these essential oils obtained from plants is used in massage,
steam, or bath. Aromatic oils are the ones, which are healable and curable. These oils are obtained
from flowers, roots, trees, fruit, seeds, crust, and leaves, and have all of the properties that refresh and
odorize plants. Humanity explored the benefit of these oils considered as the heart of plants thousands
years ago. Aromatherapy based on the fact that essential oils obtained from plants influence the
organism with olfactory receptors has a six-thousand-year-old history. Stress experienced intensively
today is known to have a negative effect on every part of human body. The studies on the after-
treatment ways against several psychological and physiological illnesses go on. Grape seeds as waste
produced by the different uses of grapes need to be made usable. Especially the rich content of grape
seeds has started to be used as an alternative in the cosmetic industry, the food industry, and the health
sector. Grape seeds, which include carbohydrate, oil, and protein, have the rich vitamin, mineral,
antioxidant, and phenolic compound content. The amount of the oil in the seeds is between %10 and
% 27. This potential, which has a light and absorbable property, should be evaluated. It has been found
out by the studies made that grape seeds have such effects as decreasing the level of cholesterol,
protecting against the harmful influences of medicine and chemicals, reducing the impact of UV lights
on the skin, having antimicrobial, anti-carcinogenic, antioxidant, and cardiovascular influences,
improving eyesight, protecting against oedema after the operations, and renewing cells. Grape seed oil
is used as essential oil and for treatment purposes because it is fluid, rich in fatty acids (omega 3,
omega 6, omega 9), and has a high amount of vitamin E which can penetrate into the human skin
easily and moisturizes. It is also used as bath and massage oil. The areas and amount of usage of grape
seed oil in aromatherapy should be increased.

KEYWORDS
V. vinifera L.; aromatherapy, grape seed oil

Corresponding Author: M. LHAN ODABAIOLU, milhanodabasioglu@gmail.com


1
MUNZUR NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM, TUNCEL
2
HARRAN NVERSTES, ZRAAT FAKLTES, BAHE BTKLER BLM, ANLIURFA
3
BNGL NVERSTES, ZRAAT FAKLTES, BAHE BTKLER BLM, BNGL

1745
Poster Session 14
Submission ID: 605

IMPORTANT TO HUMAN HEALTH, SOME BUSH FRUITS


INVESTMENT POTENTIALS IN TUNCELI

1 2 3
NESRIN KARACA SANYREK , M. LHAN ODABAIOLU , ATILLA AKIR

ABSTRACT
With the growing population on earth, the current resources are gradually decreasing and the
destruction of nature is consequently increasing. However, our bush fruits, so valuable in terms of
health, together with the endemic species are among the most affected ones from this destruction. Our
country is quite fertile for the potential species with regard to bush fruits. There are many bush fruits,
some of which have been unexplored or ignored though they have been used for some specific
purposes. These fruits, particularly important to human health, are facing the danger of extinction
because of the decrease in number, not being taken under protection, or the inadequate growth.
Restoring of already extinct species is too difficult or even impossible. Tunceli, a city in the east of
Turkey, is quite rich in bush fruits. Rich in nutrition, important to human health, these fruits are quite
suitable for the processing industry. Bush fruits, easily grown in the infertile lands and under harsh
climate conditions, have been known for having protective effects against many diseases and have
been traditionally used against illnesses for years. Bush fruits and its rich potential in Tunceli wait to
be explored. The richness of the district, the conditions of which are adaptable to the farming of
Bullace (kara mamuk), rosehip (ku burnu), blackberry (brtlen), raspberry (ahududu) highbush
cranberry (gilaburu), thorn apple (al), should be realized and taken into consideration.

KEYWORDS
Bush fruits; New investment plants; Tunceli; Human health

Corresponding Author: M. LHAN ODABAIOLU, milhanodabasioglu@gmail.com


1
MUNZUR NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM, TUNCEL
2
HARRAN NVERSTES, ZRAAT FAKLTES, BAHE BTKLER BLM, ANLIURFA
3
BNGL NVERSTES, ZRAAT FAKLTES, BAHE BTKLER BLM, BNGL

1746
Poster Session 14
Submission ID: 639

HEALING TREE: COMMON WITCH HAZEL (HAMAMELIS


VIRGINIANA)

1 1
MGE HSANE ZCAN , HEDA BASIRE AKA

D
ABSTRACT
HEALING TREE: COMMON WITCH HAZEL (Hamamelis virginiana) There are

TE
approximately 100 species of Hamamelis in the world. Due to fallen leaves in the autumn and it
started to bloom in the winter, they are called "Witch hazel," "Magic nut", "Magic walnut" and "Magic
bush". Generally theirs species are grown in North America (H. mexicana, H. ovalis, H. virginiana and
H. vernalis), Japan (H. japonica) and China (H. mollis). Of these, only as healing Hamamelis
virginiana is used for medical purposes. One of the American species, the common witch hazel
EN
(Hamamelis virginiana) plant has been spread from South Florida to eastern Texas, western Nova
Scotia to Minnesota. This plant is a tree that grows in forestland endemic to North American. It is also
grown in Europe and semi-tropical forests. This plant, which is English Witch Hazel is also known as
Bitter Hazel and Pigeon Tree in Turkey. The leaves and shells of Hamamelis is used for making tea,
ES
bandages, suppository, tincture and natural medicines. The most important effect of Hamamelis has a
natural astringent namely firming feature. This is one of the most preferred plants in the cosmetic
industry with this feature. In this study, have been given general characteristics of the common witch
hazel, their usage areas, information obtained from various references about the studies.
PR

KEYWORDS
Common witch hazel, Usage areas, Hamamelis virginiana
T
O
N

Corresponding Author: MGE HSANE ZCAN, imgeiozcan@beun.edu.tr


1
BLENT ECEVT NVERSTES MTHAT-MEHMET ANAKI MESLEK YKSEKOKULU

1747
Poster Session 14
Submission ID: 994

HONEY PLANTS OF ORUMCEK FOREST : EVALUATING


MEDICINAL AND AROMATIC PLANTS FOR CULTIVATION

1 2
GLAH YILMAZ , GLSM YALDIZ

ABSTRACT
Flora has a significant effect on characterization of honey in beekeeping. rmcek Forest or
Spider Forest is known possess highly rich plant diversity that including many medicinal and aromatic
honey plants. Nearly 620 plants taxa were identified totally over 38 thousand hectares which of 263
hectares are protected in the Forest. In recent years, some regional projects are being implemented the
medicinal and aromatic plants and to develop beekeeping by the local people and increasing
employment in the Black Sea Region. Some medicinal and aromatic plants which are grown in the
rmcek Forest have been cultivated as part of a project and they have been growing in the Krtn
district of Gmhane Province since 2012. In this study, information is provided about the medicinal
and aromatic plants that can be cultivated as a honey plant by flora trips in the rmcek Forest.

KEYWORDS
Flora, honey plant, cultuvation, medicinal and aromatic plants, rmcek Forest

Corresponding Author: GLAH YILMAZ, ylmzgulsah@gmail.com


1
KARADENZ TEKNK NVERSTES BYOLOJ BLM
2
ABANT ZZET BAYSAL NVERSTES TARLA BTKLER BLM

1748
Poster Session 14
Submission ID: 1029

IMPORTANTANCE OF GINGER (ZINGIBER OFFICINALE ROSCOE)


FOR HEALTH

1 1 1
HLYA VATANSEVER , DUYGU ERCAN ORU , ABDULLAH ALAR

ABSTRACT
Ginger (Zingiber officinale Roscoe), a member of the family Zingiberaceae, has been used as
a spice and medicinal plant in many parts of the world for centuries. Many civilizations have been
used ginger in various forms in the treatment of respiratory and urinary tract infections, stomach and
intestinal disorders, nervous disorders, diabetes, infertility, headache, toothache and rheumatism.
Carbohydrates, amino acids, vitamins (A, B3, B6) and minerals (Ca, Fe, Mg, Mn, P, K, Na, Zn)
constitute the nutrient content of ginger. In addition, ginger has shogaol and gingerol polyphenolic
substances with high antioxidant properties. Researches have shown that ginger has numerous
therapeutic properties including antioxidant, anti-inflammatory, antiplatelet, antimicrobial, antiemetic,
radioprotective, gastrokinetic, cologne effects. It has been determined that ginger is effective against
various gastrointestinal cancers such as stomach, liver, pancreas and colonic carcinoma and
cholangiocarcinoma. Modern clinical trials have shown that ginger helps to relieve nausea and
vomiting to reduce muscle pain and discomfort and respiratory tract infections, and protects the
digestive system and brain health. It also reduces LDL cholesterol and increases HDL cholesterol,
regulating insulin levels. The results obtained from scientific data reveal that ginger supports most of
the purposes of uses for various reasons among the people. The fact that ginger has a rich nutritional
content and too many health benefits to mention increases the importance of spreading consumption of
ginger.

KEYWORDS
Medicinal plant, ginger, antioxidant, anti-inflammatory, health

Corresponding Author: HLYA VATANSEVER, vatanseverhulya@hotmail.com


1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1749
Poster Session 14
Submission ID: 1559

THE DPPH FREE RADICAL SCAVENGING ACTIVITY AND ABTS


CATION RADICAL DECOLORISATION ACTIVITIES OF ETHANOL
EXTRACTS OF SEEDED RAISINS
1 1 2 2 3
DILEK DEIRMENCI KARATA , VEYSI Z , MEHMET AKDENZ , IIL AYDIN , ABDLSELAM ERTA

ABSTRACT
The DPPH Free Radical Scavenging Activity And ABTS Cation Radical Decolorisation
Activities Of Ethanol Extracts of Grape Raisin Seeds Dilek Deirmenci KARATA1 Veysi Z1
Mehmet AKDENZ2 Il AYDIN2 Abdulselam ERTA3 1 Dicle University, Faculty of Agriculture,
Department of Horticulture, Diyarbakr, TURKEY 2Dicle University, Faculty of Pharmacy,
Department of Analytical Chemistry, Diyarbakr, TURKEY 3Dicle University, Faculty of Pharmacy,
Department of Pharmacognosy, Diyarbakr, TURKEY SUMMARY Considering the changes in
nutrition recommendations and models in the world, the seeded raisins should be considered as one of
the products with significant potential for foreign trade. In recent years, the demand for core black
raisins, which are constantly on the agenda in terms of healthy nutrition due to their blood-building
and high antioxidant content, has increased considerably. Anthocyanins are the most common
phenolic compounds in red grapes and flavonols are most commonly present in white grapes. The
health benefits associated with fresh grape consumption are broadly known and linked to the richness
of phenolic compounds.These compounds have been demonstrated to have a wide range of
biochemical and pharmacological effects, including anticarcinogenic, and antiatherogenic,
antiinflammatory, antimicrobial, and antioxidant activities. In this study, seeds of variety of grape
varieties of Besni, Dmk, Rumi, Banaz siyah, Horoz Karas, Kerk grapes collected from
Southeastern Anatolia Region of our country were studied. The material (4-8 g) was dried and
powdered, and they were macerated with ethanol (25 mL 3) for 24 h at 25 C. After filtration, the
solvents were evaporated to obtain crude extracts. The ethanol extracts of seeds were tested for
antioxidant (DPPH free radical scavenging activity and ABTS cation radical decolorization) activities
in this study. Studied six extracts were found to be quite active on both antioxidant activity methods.
In particular, the ethanol extract of Banaz Black (BBS) showed 86.34% inhibition in 50 g / ml
concentration and 87.79% in ABTS method in DPPH method. In both methods, it was found that the
Dmk grape seed samples (DS) showed lower activity than the others. In our study, it was
determined that antioxidant potentials of different grape varieties were quite different. We are
continuing our efforts to determine the chemical components that cause this. Acknowledgements: The
research was funded by grant : DUBAP from Dicle niversity Keywords: Vitis vinifera ssp. sativa,
seeded raisins, seeds, DPPH, ABTS

KEYWORDS
Vitis vinifera ssp. sativa, ekirdekli kurutmalk zm, zm ekirdei, DPPH, ABTS

Corresponding Author: DLEK DERMENC KARATA, dilek76@gmail.com


1
DCLE NVERSTES ZRAAT FAKLTES BAHE BTKLER BLM
2
DCLE NVERSTES ECZACILIK FAKLTES ANALTK KMYA BLM
3
DCLE NVERSTES ECZACILIK FAKLTES FARMOKOGNOZ BLM

1750
Poster Session 14
Submission ID: 1753

THE BIOLOGICAL ACTIVITIES OF LAVANDULA STOECHAS L.


AGAINST FOOD PATHOGENS

1 1 1 1
DUYGU BAYRAK , GLTEN KMEN , AL ARSLAN , MUSTAFA VURKUN

ABSTRACT
Foodborne pathogens are microorganisms as well as a number of parasites, which are capable
of infecting humans via contaminated food or water. In recent years, diseases caused by foodborne
pathogens have become an important public health problem in the world, producing a significant rate
of morbidity and mortality. In traditional medicine, numerous plants and their extracts have used for
thousands of years to treat health disorders. Although many studies were made on natural herbs, those
involving the antimicrobial, antioxidant and antimutagenic activity of the herb species are rather rare.
This study researches the biological activities of ethanol and methanol extracts of Lavandula stoechas
L., which are prevalent in Turkey. In this study, 8 food pathogens were used for antimicrobial activity
studies. Antimicrobial activity studies were done by disk diffusion assay and MIC (minimum
inhibitory concentration). DPPH method was used for non-enzymatic antioxidant activity. The
Lavandula extracts were screened for their antimutagenic activity against sodium azide by Ames test
in absence of rat microsomal liver enzyme (-S9). The ethanol and methanol extracts of Lavandula
stoechas showed antibacterial activity (7 mm) against most of bacteria. The antifungal activity of L.
stoechas was not determined against C. albicans RSKK02029. The lowest MIC value was determined
as 3250 g/mL. The highest radical inhibition was determined as 79 % by Lavandula stoechas flower
methanol extract. The flower extract of L. stoechas (12500 g/plate) was found to have its highest
antimutagenic activity for Salmonella Typhimurium TA98. This inhibition value is 42 %. L. stoechas
leaves extracts (6250 and 3125 g/plate) showed a moderate positive inhibitory effectfor Salmonella
Typhimurium TA98, and TA100. L. stoechas flower extracts (12500 and 6250 g/plate) showed a
moderate positive inhibitory effect (respectively 31 and 30 %) for Salmonella Typhimurium TA100.
The extracts of L. stoechas have antimicrobial, antioxidant and antimutagenic activities.

KEYWORDS
Lavandula, Antimicrobial Activity, Antioxidant Activity, Antimutagenic Activity

Corresponding Author: AL ARSLAN, aliarslan47@gmail.com


1
MULA SITKI KOMAN NVERSTES

1751
Poster Session 14
Submission ID: 1755

EFFECT OF INFUSION TIME AND CONCENTRATION ON


ANTIMICROBIAL AND ANTIOXIDANT POTENTIAL OF
LAVANDULA STOECHAS L. TEAS

1 1
SIBEL AYDIN , PINAR KADIROLU

ABSTRACT
Lavandula stoechas L. that belongs to Lamiaceae family is one of the medicinal and aromatic
plants, especially grown in the Mediterrenean Region. The antiseptic effect of this plant with emphasis
on treatment of several diseases were reported. Recently, the great concern on natural products and the
plants like this has increased. Studies on Lavandula stoechas L. were mostly performed on
antibacterial properties of volatile oils of this plant. However, there was no study on optimization of
infusion process that were related with antimicrobial and antioxidant potential of Lavandula stoechas
L. teas. In this study, the effects of different infusion times and concentrations on antimicrobial and
antioxidant potential of teas were investigated. For this purpose, teas were infused at 10% and 20%
(w/v) concentrations for 5, 10 and 20 minutes. Antimicrobial activity test was conducted with Kirby-
Bauer disc diffusion assay by using Staphylococcus aureus and Escherichia coli as test organisms and
zones of inhibition of teas were determined. Antioxidant potential of teas was performed with DPPH
radical scavenging activity assay. According to the results, antimicrobial activities of teas infused for
10 to 20 minutes were found to be similar and higher than infusion time of 5 minutes. When the effect
of infusion concentrations were compared, antimicrobial acitivity teas with 20% (w/v) was higher than
10% (w/v) concentration. Antimicrobial potential of Lavandula stoechas L. teas was more effective on
E. coli rather than S. aureus. Antioxidant potentials of the teas were comparable with antimicrobial
activities and teas with higher infusion concentration and 10 minutes infusion time had better
antioxidant potential when compared to other infusion conditions. The results revealed that
optimization of infusion conditions of teas were important for remarkable inhibiton against pathogens
and antioxidant potential.

KEYWORDS
Lavandula stoechas L., infusion, antimicrobial, antioxidant

Corresponding Author: PINAR KADROLU, pkadiroglu@adanabtu.edu.tr


1
ADANA SCENCE AND TECHNOLOGY UNVERSTY

1752
Poster Session 14
Submission ID: 1756

THE BIOLOGICAL ACTIVITIES OF SILYBUM MARIANUM L.


GAERTN. AGAINST FOOD SPOILAGE MICROORGANISMS

1 1 1
DUYGU BAYRAK , GLTEN KMEN , AL ARSLAN

ABSTRACT
Food safety and foodborne pathogenic microorganisms are one of the major public health
problems in the globalizing world. In the world, numerous plants and their extracts are used for
thousands of years to treat health disorders. This study researches the biological activities of extracts
of Silybum marianum, which is prevalent in Turkey. In this study, 7 bacteria and 1 yeast were used for
antimicrobial activity studies. Antimicrobial activities studies were done by disk diffusion assay and
MIC (minimum inhibitory concentration). Antioxidant activities were studied by DPPH method. The
plant extracts were screened for their antimutagenic activity against sodium azide by Ames test in
absence of rat microsomal liver enzyme (-S9). The extracts of Silybum marianum showed strong
antibacterial activity (10 mm) against Bacillus subtilis RSKK245 and Staphylococcus aureus
RSKK2392. The highest antifungal activity was obtained methanol extract of Silybum marianum
flowers against Candida albicans RSKK02029 (9 mm). The lowest MIC value was found as 3250
g/mL. The highest radical inhibition was determined as 72 % by Silybum marianum flower methanol
extract. Silybum marianum flower extracts (12500 and 6250 g/plate) showed a mutagen effect for
Salmonella Typhimurium TA98. However, these extracts showed low antimutagenic activity for
Salmonella Typhimurium TA100. Silybum marianum leaves extracts (6250 and 3125 g/plate)
showed a moderate positive inhibitory effect for Salmonella Typhimurium TA98, and TA100
(respectively 38 and 28 %). The leaves and flowers extracts of Silybum marianum have antimicrobial,
antioxidant and antimutagenic potential.

KEYWORDS
Silybum, Antimicrobial Activity, Antioxidant Activity, Antimutagenic Activity

Corresponding Author: AL ARSLAN, aliarslan47@gmail.com


1
MULA SITKI KOMAN NVERSTES

1753
Poster Session 14
Submission ID: 1758

ESSENTIAL OIL COMPOSITION AND ANTIFUNGAL AND


ANTIBACTERIAL ACTIVITY OF ACHILLEA CAPPADOCICA

1 1 1 2 1 1
NUSRET GEN , HSEYIN AKT , MAHFUZ ELMASTA , ZEYNEP AKT , SA KARAMAN , BEDRETTIN SELV

ABSTRACT
Achillea species is perennial herbs belongs to Asteraceae family and has a characteristic odor.
The genus Achillea is represented 41 species and 7 subspecies 24 of which are endemic to Turkey
according to Flora of Turkey. Achillea cappadocica is one of the endemic species and its essential oil
compositions has not been reported previously. Achillea species are known as civan peremi in
Turkish and used as a folk medicine. A large number of Achillea species are on the "generally
recognized as safe plants list (GRASS) in Turkey and marketed for treatment of various diseases.
This study focused on the determination of essential oil composition of A. cappadocica. Plant
materials were collected from Refahiye, Erzincan at June 2016. Essential oils were obtained using
clevenger type apparatus during 30 min and analyzed with GC-MS instrument. 27 compounds were
identified representing %97.4 of total oil. The major components were found as ascaridiol (%55.5)
isoascaridiol (%10.3), p-cymene (%11.17) and eucalyptol (%6,56). Ascaridiol is a naturally occurring
endoperoxide monoterpene found in essential oil of Chenopodium ambrosioides (Mexican tea).
Ascaridiol and isoascaridiol have unknown toxicity. It was reported the occurrence of ascaridiol in
Achillea millefolium growing northern Greece as major component by % 47 [1]. When compared
other Achillea species growing in Turkey, the being large amount of ascaridiol in Achillea
cappadocica is remarkable. To ensure identifying ascaridiol, 1H and 13C-NMR spectrums of crude
essential oil were recorded in CDCl3 due to suspicious MS library hits. Two olefinic methine (136.3
C2 and 133.0 C3), one aliphatic methine (32.1 C8), two oxygenated quaternary (79.7 C4 and 74.3 C1),
three methyl (21.3 C7, 17.2 C9, 17.1 C10) , two aliphatic methylene (29.5 C6, 25.6 C5) signals were
observed at 13C-NMR spectrum with fully agreement with literature data[2]. The antifungal and
antibacterial activity of crude essential oil were determined using disc diffusion method. Essential oil
exhibited great activity against P. aeruginosa, E. faeccalis and S. aureus and moderate activity against
E. coli and C. albicans when compared commercially available some antibiotics such as tetracycline,
gentamycin and streptomycin. 1. Chatzopoulou, P., S.T. Katsiotis, and A.B. Svendsen, An ascaridole
containing essential oil of the Achillea millefolium L. complex growing wild in northern Greece.
Journal of Essential Oil Research, 1992. 4(5): p. 457-459. 2. Cavalli, J.F., et al., Combined analysis of
the essential oil of Chenopodium ambrosioides by GC, GC MS and 13C NMR spectroscopy:
quantitative determination of ascaridole, a heat sensitive compound. Phytochemical Analysis, 2004.
15(5): p. 275-279.

KEYWORDS
Achillea cappadocica, Antifungal Activity, Antibacterial Activity, Essential Oil

Corresponding Author: NUSRET GEN, nusretgenc@gmail.com


1
GAZOSMANPAA NVERSTES
2
GMHANE NVERSTES

1754
Poster Session 14
Submission ID: 1760

ISOLATION BIOLOGICAL COMPOUNDS FROM TRICHOLOMA


FOCALE

1 1 1
MEHMET HSEYIN SINGE , ERHAN KAPLANER , MEHMET ZTRK

ABSTRACT
Tricholoma species are generally favorable in most of the Asian countries among the others
because of their marvelous tastes. Anatolia has diversity of Tricholoma species. Tricholoma
anatolicum is considered as a new matsutake belonging to Anatolia peninsula. Tricholoma focale is
another mushroom which is widely found in conifer forests of Anatolia. Having the diverse biological
activity and being an important source of food, these mushrooms have very broad investigation field
(Zaidman, Yassin, Mahajna, & Wasser, 2005). The aim of this study is to isolate biologically active
compounds from Tricholoma focale. For this purpose the mushroom was extracted using methanol.
Silica gel column chromatography, thin layer chromatography and Recycling prep. HPLC were
employed for isolation studies. As a result, 1 new steroid along with 3 known compounds (2-4)
isolated. 1 2 3 4 Acknowledgements: This study is supported by TUBITAK-SBAG with the Project
number of 113R012. References: Zaidman, B.-Z., Yassin, M., Mahajna, J., & Wasser, S. P. (2005).
Medicinal mushroom modulators of molecular targets as cancer therapeutics. Applied Microbiology
and Biotechnology, 67(4), 45368. http://doi.org/10.1007/s00253-004-1787-z

KEYWORDS
Tricholoma focale, biological compounds, isolation, NMR spectroscopy

Corresponding Author: MEHMET HSEYN SNGE, singecmh@gmail.com


1
MULA SITKI KOMAN NVERSTES

1755
Poster Session 14
Submission ID: 1761

EFFECTS OF POLYPHENOLS ON DIGESTIBILITY OF


MACRONUTRIENTS
1 2
YUSUF DURMU , MNIR ANIL

ABSTRACT
Polyphenols are dietary constituents of plants associated with health-promoting effects which
are generally consumed with macronutrients in the human diet. Some polyphenols found in foods
include catechins, condensed tannins, anthocyanidins and some phenolic acids such as caffeic acid,
ferulic acid, phytic acid, gallic acid, etc. The effect of interactions between food macronutrients and
plant phenols is a very important topic because of their bioavailability that effect on human health.
Polyphenolic compounds have capacity to bind and precipitate proteins. Low-molecular-weight
phenols are unable to precipitate protein, and oligomers must contain at least three flavonol subunits to
effectively precipitate protein. The proteinpolyphenol complexes do not affect the absorption of
polyphenols but can significantly change the plasma kinetics profile. Moreover, tannins can bind and
inhibit other endogenous proteins in the intestinal tract, such as digestive enzymes. This leads
reduction not only to the digestibility of the protein but also to other macronutrients such as starch and
lipids. Likewise, polyphenols also can form complexes with polysaccharides other than those that
form the plant cell wall (i.e., starch) and also affect the glycemic and insulinemic responses.
Carbohydrates can increase absorption and prolong the time required to reach the maximum
polyphenol concentration. Interaction of phenolic acid with starch significantly contributes to the
inhibitory effect of starch hydrolysis. Increased fermentation of oligosaccharides in the large intestine
increases polyphenol bioavailability. Furthermore, the fermentation of oligosaccharides enlarges the
intestinal mucosa, suppresses the bacterial degradation of the polyphenols in caecum, and also
influences the mucosal blood flow by stimulating mucosal blood flow, thus contributing to polyphenol
absorption in the intestine. In addition to its effect on absorption, it has been observed that
carbohydrates alter the plasma kinetic profile of polyphenols. A positive correlation was observed
between fat concentration and bioavailability of polyphenols after in vitro gastropancreatic digestion.
Oils can change the absorption kinetics of polyphenols and increase their absorption. Hydrophobic
interactions between oils and polyphenols promote the stability of polyphenols during digestion and
therefore high fat content in the food have a protective effect. Bioavailability of phenolic compounds
has been determined to increase after medium-chain fatty acid and long-chain fatty acid diets
compared to standard diets. In addition, the degree of saturation of the oils can affect the
bioavailability of the polyphenols. Furthermore, not only a food ingredient but also certain synergies
between food macro nutrients have an important effect on the bioavailability and bioactivity of
polyphenols.

KEYWORDS
Polyphenols, macronutrients, digestibility

Corresponding Author: MNR ANIL, munira@omu.edu.tr


1
ORDU NVERSTES ZRAAT FAKLTES GIDA MHENDSL BLM
2
ONDOKUZMAYIS NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1756
Poster Session 14
Submission ID: 1762

EFFECT OF GROUNDING PROCESS ON ANTIOXIDANT AND


ANTIMICROBIAL POTENTIAL OF CARDAMOM EXTRACTS

1 1
SIBEL AYDIN , PINAR KADIROLU

ABSTRACT
Cardamom, dried fruits of Elettaria cardamomum belongs to Zingiberaceae family. Cardamom
is mostly harvested in southern India, Sri Lanka, Tanzania and Guatemala. It is traditionally used in
various gastrointestinal, cardiovascular and neuronal disorders. The dried fruit of cardamom is used
either whole or ground form as a food additive and flavouring agent. Cardamom has unique aromatic
and sharp flavour. The cardamom flavour used in processed foods, is mainly provided by the
cardamom essential oil. In this study, cardamom as whole and ground form was extracted with boiled
water at 20% (w/v) concentration for 10 minutes. Antimicrobial activities of extracts were assessed
with Kirby-Bauer disc diffusion assay by using Staphylococcus aureus, Escherichia coli and
Pseudomonas aeruginosa as test organisms and zones of inhibition of extracts were determined.
Antioxidant potential of extracts was performed with DPPH radical scavenging activity assay.
According to the results, ground cardamom extracts had possessed better antioxidant potential than
whole cardamom extracts. Antimicrobial activity studies revealed that all extracts had no antimicrobial
potential against tested microorganisms.

KEYWORDS
Cardamom, grounding, extraction, antimicrobial, antioxidant

Corresponding Author: PINAR KADROLU, pkadiroglu@adanabtu.edu.tr


1
ADANA SCENCE AND TECHNOLOGY UNVERSTY

1757
Poster Session 14
Submission ID: 1763

THE EFFECTIVENESS OF HERBAL TEAS ON GLYCEMIC


CONTROL

1 1 2
GLAH KANER , EZGI BELLIKCI KOYU , HALIL KOYU

ABSTRACT
Diabetes is a chronic disease which is related with impaired insulin secretion or utilization.
Type 2 diabetes, is a serious global health problem and its prevalence is rapidly increasing. According
to the Turkish Epidemiology Survey of Diabetes, Hypertension, Obesity and Endocrine Disease II
(TURDEP-II) study, diabetes prevalence was reached to 13.7% in adults. Due to the chronic nature of
diabetes and its effects on quality of life, people are interested in herbal medicine to reduce symptoms
of disease. According to the ethnobotanical studies, approximately 200 taxa have been used to control
blood glucose level in Turkey. Additionally, many plants such as cinnamon and ginger is popular
among diabetes patients in Turkey although they are not native to our flora. Plants are mostly prepared
by infusion or decoction methods in traditional medicine, which are commonly called as tea. Besides
its therapeutic purpose, herbal teas are used as a part of daily nutrition and they increase the quality of
with their polyphenolic content. Due to the high patient compliance and easy preparation and use,
herbal teas can be consumed as a practical complementary therapy in daily nutrition. However,
suggesting these products in clinics for glycemic control requires detailed information about their
effectiveness and safety. In line with this objective, in this study, some plants commonly used for
glycemic control such as Camellia sinensis (L) Kuntze, Cinnamomum zeylanicum Blume, Matricaria
chamomilla L., Morus alba L., Morus nigra L., Rosa canina L, Salvia fruticosa Mill., Urtica dioica L.
and Zingiber officinalis Roscoe are reviewed regarding the scientific literature.

KEYWORDS
Diabetes, herbal teas, glycemic control

Corresponding Author: EZG BELLKC KOYU, ezgi_bellikci@hotmail.com


1
IZMR KATP CELEB UNVERSTY, FACULTY OF HEALTH SCENCE, DEPARTMENT OF NUTRTON AND
DETETCS, IZMR
2
IZMR KATP CELEB UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL
BOTANY, IZMR

1758
Poster Session 14
Submission ID: 1764

TRADITIONAL USE, PHARMACOLOGY AND TOXICOLOGY OF


FERULA L. SPECIES: A REVIEW

1 2
HAKIYE ASLAN , ELIF YILDIRIM

ABSTRACT
Ferula L. is the third largest species of the Apiaceae family and consists of 180-185 varieties
of flowering plants that grow in central and southern western Asia, the Far East, North India and the
Mediterranean Basin. A total of 27 Ferula species (23 species and 4 subspecies), of which 9 are
endemic, grow in the mountainous region of Eastern Anatolia, Central Anatolia, Southeast Anatolia
and Eastern Mediterranean in Turkey. Some of species of genus Ferula are used as spices, while others
are used in the preparation of local drugs to treatment of circulatory system disorders, strengthening
nerves and muscles, muscle pain, bone erosion and infertility. Also, in recent years, it has been found
that some species of Ferula genus exhibit antidiabetic, antimicrobial, anti-tumor and antiangiogenic
activities according to the in vitro and in vivo studies. However, it has been found that some Ferula
species, such as F.communis, have two different types of chemotypes that are toxic and non-toxic. The
toxic chemotype shows anticoagulant and cytotoxic effects with sesquiterpene coumarins as main
components while the main component of non-toxic chemotype is the daucane sesquiterpene esters
and has an estrogenic effects. In this study, the available information about the traditional use,
pharmacology and toxicology of Ferula L. species is summarized and its potential therapeutic effect,
medical and economic importance has been mentioned.

KEYWORDS
Ferula L., pharmacology, toxicology

Corresponding Author: HAKYE ASLAN, hakiye@hotmail.com


1
BNGL NVERSTES
2
NECMETTN ERBAKAN NVERSTES, MERAM TIP FAKLTES HASTANES

1759
Poster Session 14
Submission ID: 1765

EFFECTS OF VARIOUS DRIED POLLEN SAMPLES ON BIOFILM


FORMATION IN P. AERUGINOSA

1 2 1
HALIME EVKBA , BEKIR GKEN MAZI , SEYHAN ULUSOY

ABSTRACT
Pseudomonas aeruginosa is an opportunistic human pathogen which forms biofilm through
quorum sensing system. Repressing biofilm formation will be a treatment approach that looks
promising especially for the immunocompromised patients. Hence, a variety of pollen samples that are
known to be beneficial for human health and have been used for this purpose for a long time were
tested. The effects of the five pollen samples that were exposed to different drying processes on
biofilm formation in P. aeruginosa were investigated. In conclusion, it was observed that the frozen
pollen sample (DP) inhibited biofilm formation by %62 with maximum rates. At the end of this
research, it was showed that pollens can be a significant anti-virulant agent by suppressing biofilm
formation.

KEYWORDS
Pollen, biofilm, P. aeruginosa.

Corresponding Author: HALME EVKBA, halimecevikbas@gmail.com


1
SLEYMAN DEMREL NVERSTES
2
ORDU NVERSTES

1760
Poster Session 14
Submission ID: 1767

ANTIOXIDANT ACTIVITY IN VARIOUS EXTRACTS OF


HAPLOPHYLLUM PTILOSTYLUM AND ALCEA ACAULIS

1 1 1 1 1
ABDURRAHMAN AKDAG , ABDULLAH AYKANAT , DERYA AKIEK , ISMAIL KOYUNCU , MESUT IIK ,
6
MAHMUT PADAK

ABSTRACT
Many pathological disorders such as arthrosclerosis, diabetes and cancer are associated with
reactive oxygen species. Antioxidants protect the cells from harmful effects of free radicals and
reactive oxygen species. Therefore, the detection of antioxidant rich plants is important for preventing
and treating the oxidation of cells. The present study was conducted to determine the total
phenolic/flavonoid content, antioxidant activitiy of methanol, buthanol, dichlorometane, water and
hexane extracts prepared from Haplophyllum ptilostylum and Alcea acaulis. Antioxidant activity of
the sample was comparable to commercial antioxidant standards (BHT, askorbik asit and trolox). The
results showed that extracts prepared with different solvents has moderate free radical scavenging and
reducing capacity.

KEYWORDS
Alcea acaulis, Antioxidant, Haplophyllum ptilostylum, reactive oxygen species

Corresponding Author: MESUT IIK, mesutisik16@gmail.com


1
HARRAN UNVERSTY

1761
Poster Session 14
Submission ID: 1769

FUNCTIONAL ACTIVITY OF ISPARTA ROSE ( ROSA DAMASCENA


MILL.) AND HEALTH EFFECTS

1 1
SANIYE SZL , EFSUN KARABUDAK

ABSTRACT
It has been reported that mutations are an important cause in the pathogenesis and progression
of many diseases such as atherosclerosis, heart disorders and cancer. For this reason, anti-mutagens
from natural dietary sources that can resist the promutogenic and carcinogenic effects of mutagens
have a great deal of precaution. Several phytochemicals have been reported to provide health
protective effects through their anti-microbial, antioxidant, anti-mutagenic, anti-carcinogenic and anti-
inflammatory activities. It is known that a large part of these health effects are carried out through
phenolics and flavonoids in phytochemicals. The benefits derived from the use of natural products rich
in bioactive substances have increased the growing interest of pharmaceutical, food and cosmetic
industries. Roses are also used in perfumes, pharmaceuticals and food industries as well as being used
as an ornamental plant in parks, gardens and houses, with over 150 species and more than 20,000
cultivars. The Rosa genus is composed of many species and different regions have their own endemic
species. Rosa Damascena Mill. (Damask rose, Oil-bearing rose, Pink rose) is the most important
species, producing a high-value aromatic oil, which is used in the pharmaceutical and fragrance
industries. Turkey is one of the most important country growing Rosa damascena in the world. Several
components were isolated from the flowers, leaves and seeds of R. damascena including terpenes,
glycosides, flavonoids and anthocyanins. It also contains carboxylic acid, vitamin C, kaempferol and
quercetin and organic acids. It has attracted attention in recent years as a medical food ingredient due
to anti-inflammatory, antioxidant and cyclooxygenase inhibition activities as well as essential oil and
pharmaceutical effects produced. The rose seed extract has been shown to have antioxidant and anti-
microbial activities. Generallay, the extent of the antioxidant and antibacterial effects of the extracts
could be attributed to their phenolic compounds and essential oil contents. According to the results,
the extracts obtained from fresh Rosa damascena flowers have higher antioxidant and anti-bacterial
activity than the dried ones. It is reported that roses may be one of the important functional foods with
the positive effects on health. For this reason, rose extracts may have potency to be used as a natural
antioxidant source in healthy products for the prevention of oxidative damage and for the inhibition of
the progression of certain diseases (heart diseases, cancer, diabetes). However, the food sources that
currently contain roses on the market are products of high sugar content, such as Turkish delight, jam.
Consumption can be supported by developing healthy foods.

KEYWORDS
Rosa damascena, phenolics, health effects

Corresponding Author: SANYE SZL, saniyekoyuncu05@gmail.com


1
GAZ NVERSTES, SALIK BLMLER FAKLTES, BESLENME VE DYETETK BLM

1762
Poster Session 14
Submission ID: 1770

EFFECTS OF CURCUMIN ON BREAST CANCER

1 2 1
NAZLI NUR ASLAN , MELDA KANGALGL , AYE ZFER ZELK

ABSTRACT
Curcumin (diferuloylmethane) is a polyphenol obtained from the Curcuma longa plant,
commonly known as turmeric. Approximately 2.0-5.0% of curcumin is curcuminoid and it is
responsible for the the yellow color of the spice and its therapeutic effects. Curcumin has various
biological and pharmacological effects such as antioxidant, aniinflammatory, antiviral, antiischemic,
antibacterial, antifungal, immunomodulator, antiproliferative and anticancer. Curcumin is helpful in
reducing post-operative inflammation and in preventing atherosclerosis. In addition, curcumin also
prevents the development of Helicobacter pylori, which causes gastric ulcer and is associated with
gastric cancer. Curcumin can bind to heavy metals such as cadmium and lead, and thus reduce the
toxicity of these heavy metals. Studies showed that curcumin does not have toxic effects on humans.
Despite its strong biological and pharmacological properties, low bioavailability is the most important
problem in the clinical use of curcumin. The anticancerogenic effects of curcumin are directly due to
antioxidant and free radical scavenging effects. The underlying mechanism is still unclear, as it is
estimated that the indirect effect is due to increased levels of glutathione due to hepatic detoxification
of mutagens and carcinogens and the prevention of the formation of nitrosamine. Breast cancer is the
most common type of cancer seen in women and the prevalence in developed countries accounts for
about 25.0% of the types of cancer seen in women. Breast cancer in the world is the second most
important cause of cancer-related deaths in women. Surgical treatment, radiation, chemotherapy,
immunotherapy, hormone therapy and stem cell transplantation are used in breast cancer treatment.
These treatments have a significant impact on the quality of life of the patient with the cost of having
expensive and serious side effects. Breast cancer is resistant to chemotherapy and alternative therapies
are needed because the side effects of treatment are high. In vivo and in vitro studies showed that
curcumin inhibited proliferation of the breast cancer cell line (MCF-7), increased apoptosis and
reduced reactive oxygen species and nitric oxide levels. Curcumin was also shown to inhibit tumor
growth and angiogenesis, leading to antiproliferative activity, decreased tumor volume and weight.
High levels of fatty acid synthase (FAS) expression were found in many tumors, including prostate,
breast and over-cancer, and inhibition of FAS was shown to inhibit tumor growth. Curcumin was
determined to inhibit intracellular FAS and induce apoptosis in human breast cancer. In addition to all
of these, curcumin was found to reduce the toxic effects of drugs used in breast cancer treatment. As a
result of the studies performed, curcumin appears to be an alternative way to prevent and treat breast
cancer. However, in order to be able to use curcumin in breast cancer treatment, it is necessary to use
it together with more complex technologies as well as to conduct more studies.

KEYWORDS
curcumin; cancer; breast cancer

Corresponding Author: NAZLI NUR ASLAN, nnaslan@ankara.edu.tr


1
ANKARA UNVERSTY FACULTY OF HEALTH SCENCES, NUTRTON AND DETETCS DEPARTMENT
2
BLACK SEA TECHNQUE UNVERSTY FACULTY OF HEALTH SCENCES, NUTRTON AND DETETCS
DEPARTMENT

1763
Poster Session 14
Submission ID: 1771

STUDIES ON PHYSICAL/CHEMICAL COMPATIBILITY BETWEEN


HERBAL EXCIPIENTS AND CARVEDILOL USING SPECTROSCOPIC
AND THERMAL TECHNIQUES

1 2
HALE SEILMI CANBAY , MAHMUT DOGANTRK

ABSTRACT
Studies of drug active compound-excipient suitability represent an important phase in the
preformulation stage of the development of all dosage forms. The possible physical and chemical
interactions between drugs and excipients can affect the chemical nature, the stability and
bioavailability of drugs and, consequently, their therapeutic efficacy and safety [1]. Drug excipients
compatibility studies lays a foundation in careful selection of most appropriate excipients and helps in
designing a chemically stable and effective dosage form [2,3]. Hypertension is one of the important
threat for kidney, stroke and various cardiovascular diseases. Hypertension is treated by regulating
blood pressure and electrolyte balance [4,5]. The purpose of the present investigation was to evaluate
the compatibility of Carvedilol with various herbal excipients (cellulose, sucrose, mannitol, starch) to
be used in the nanoparticle formulations utilizing the different spectroscopic and thermal techniques
such as Differential Scanning Calorimetry (DSC), Thermo-Gravimetric/Differential Thermal Analyzer
(TG/DTA) and Fourier Transform Infrared Spectroscopy (FTIR). The thermograms of the drug and
excipient were obtained using DSC (PE DSC 400). All the samples were placed in a pre-weighed
stainless steel pan and sealed carefully with a sealer supplied by the manufacturer. The sealed pan was
weighed to obtain the sample mass. Another sealed empty stainless steel pan was used as the
reference. The sample was equilibrated heated from - 20 C to 400 C at a rate of 10 C/min
Thermogravimetric analysis, derivative thermogravimetry and differential thermal analysis
measurements were made by using simultaneous TG/DTA thermal analyzer apparatus (Seiko
TG/DTA 7200). The drug and drug-excipients mixtures were carefully weighted and transferred to a

dynamic nitrogen atmosphere heating rate of 10 C/min. ZnSe-ATR equipment was used as well as
KBr pellet technique. The FTIR measurements were formed on a PE Frontier spectrometer. Table 1
Peak temperature and enthalpy values of Carvedilol herbal excipient mixtures Sample Ratio (drug-
excipient) Tpeak (C) Enthalpy(Jg1) Carvedilol - 120.74 - 104.83 Carvedilol-Cellulose 1:1 119.31 -
52.63 Carvedilol-Sucrose 1:1 120.02 - 49.11 Carvedilol-Mannitol 1:1 119.31 - 49.50 Carvedilol-
Starch 1:1 119.41 - 65.51 There was no interaction between Carvedilol-Cellulose, Carvedilol-Sucrose,
Carvedilol-Mannitol and Carvedilol-Starch. Keywords: Drug, herbal excipient, DSC, TG/DTA, FTIR
References: 1- S. Sonali, B.S. Bharate, A.N. Bharate, J. Bajaj. Excipients and Food Chem (2010) 1 3-
26. 2- P. Pandian, K . Kannan, M. Manikandan, R. Manavalan. Int J Pharm Pharm Sci (2012) 4 342-
347. 3. ME. Aulton. (2002). Pharmaceutics The scientific principles of dosage form design. Churchill
Livingstone. 4. G. Mancia, G. De Backer, A. Dominiczak, et al. Journal of Hypertension (2007) 25
1751 1762. 5. N.F. Bras, P.A. Fernandes, M.J. Ramos. ACS Catalysis (2014) 4 25872597. 6. D.

Corresponding Author: HALE SELM CANBAY, halecanbay@mehmetakif.edu.tr


1
MEHMET AKF ERSOY NVERSTES MHENDSLK MMARLIK FAKLTES
2
MEHMET AKF ERSOY NVERSTES BLTEKMER

1764
Giron, J. Pharm. Biomed. Anal (1986) 4 755770. 7. S.A. Botha, A.P. Lotter. Drug Dev Ind. Pharm
(1990) 16 673683. 8. S.Y. Lin, R.Y. Han. Pharmazie (1992) 47 266268. 9. F. Giordanoa, G.P.
Bettinetti. J Pharm Biomed Anal (1988) 6 951955. 10. K.R. Verma1, S. Garg. J Pharm Biomed Anal
(2004) 35 449458. 11. M. Tomassetti, A. Catalani, V. Rossi, S. Vecchio. J Pharm Biomed Anal
(2005) 37 949955.

KEYWORDS
Drug, herbal excipient, DSC, TG/DTA, FTIR

1765
Poster Session 14
Submission ID: 1772

THE EFFECT OF PHITOTHERAPY ON POSTPARTUM DEPRESSION

1 2
ESRA USLU , MENEKE NAZLI AKER

ABSTRACT
Objective: Postpartum depression is a serious psychological problem that weakens the link
between the mother and the baby in the postpartum period. Despite the fact that there is no clear data
on the incidence of mental illness due to fear of stigmatization, it is thought that it affects 13% of
mothers. For this reason, many mothers refuse psychological assistance because postpartum
depression is normal or has a psychiatric history. However, many drugs are also contraindicated in the
lactation process. At this point, phytotherapy may be an alternative in the management of postpartum
depression. For this reason, this study aims to determine the effect of phytotherapy on postpartum
depression. Methods: This is a review study which is studies through Pubmed, EBSCO Host, Ulakbim
Medical Database, Turkish Medline databases. During the scanning, the words "postpartum
depression" and "phytotherapy" and the Turkish equivalents of these words were used. A total of 25
articles were obtained (Pubmed n = 14, EBSCO Host n = 11, Ulakbim Medical Database n = 0,
Turkish Medline n = 0). 18 of these articles were review studies and two of these articles were about
aromatherapy treatments. So in total 20 of these articles were not examined. Since 2 articles out of 5
articles were the same studies, in total 3 articles were examined. One of the studies was carried out in
the United States, one in Iran and one in Taiwan. We did not find any study related to the subject in
our country. Results: Phytotherapy; It was the most prescribed method by nurses and midwifes in
North Carolina. Nurses and midwifes also stated that they also use phytotherapy for postpartum
depressed mothers. The results of two other experimental studies show that saffron is a safe
complementary method to improve the depressive symptoms of postpartum depression, and
chamomile tea is an additional approach to alleviate depression and sleep quality problems in
postpartum period. Conclusion: The results of this review have showed the numerical insufficiency of
the studies taken into consideration and a limited number of herbal products were examined. The
methods used were found to be effective on postpartum depression. In this context, it is suggested that
planning of descriptive studies in which the opinions of health workers and postpartum mothers on
phytotherapy are determined and the current situation is also determined, randomized controlled trials
evaluating the effect of different plants on postpartum depression.

KEYWORDS
postpartum depression, phytotherapy, nurse

Corresponding Author: MENEKE NAZLI AKER, menekseaker@gmail.com


1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEKOKULU
2
ANKARA NVERSTES SALIK BLMLER FAKLTES

1766
Poster Session 14
Submission ID: 1774

INVESTIGATION OF AROMA PROFILES OF CRAB APPLE (MALUS


TRILOBATA V ERIOLOBUS TRILOBATUS) AT DIFFERENT
TEMPERATURES AND DRYERS

1 1 1 2
SELMA KAYACAN , AHMET ABDULLAH US , OSMAN SADI , RASIM ALPER ORAL

ABSTRACT
TThe crab apple is a wild fruit which rarely grows in our country. There is a limited number of
studies about crap apple in the literature. It is generally known to the region where it grows and it is
used against cholesterol, shortness of breath, diabetes and blood pressure. In this study, crab apple was
dried in two different temperatures, 40C and 60C, in the oven and vacuum dryer, and by using GC-
MS, effect of temperatures and dryers on the aroma compounds of dried crab apple were compared.
According to the results, carvacrol, anethole and kuminaldehyde which have antioxidant effects were
found as dominant aromas in crab apple. Compared to the oven and vacuum dryer, the components
were found to be lower in oven drying, and the components of 60C were found to higher than of 40C
at oven drying. When compared 40C vacuum drying with 40 C oven drying, carvacrol was found to
about 10 times more, anethole was found about 3 times more and kuminaldehyde was found about 4
times more in in the vacuum dryer. The highest value of the all components was obtained at 40C in
the vacuum dryer. According to results; it was determined that drying temperature and type of dryer
have effect on antioxidant content of crab apple.

KEYWORDS
Crab apple, GC-MS, Aroma profile, Drying

Corresponding Author: SELMA KAYACAN, selmakayacan@gmail.com


1
YILDIZ TEKNK NVERSTES, KMYA METALURJ FAKLTES, GIDA MHENDSL BLM
2
BURSA TEKNK NVERSTES, DOA BLMLER, MMARLIK VE MHENDSLK FAKLTES, GIDA
MHENDSL BLM

1767
Poster Session 14
Submission ID: 1777

ESSENTIAL OIL COMPOSITION, ANTIOXIDANT ACTIVITY AND


ANTI-PROLIFERATIVE EFFECT OF A NEW SPECIES:
PARACARYUM BINGOELIANUM

1 2 1 1
MEHMET KADIR ERDOAN , HAKAN AKIN , LTFI BEHET , BRAHIM HALIL GEBESLER

ABSTRACT
The plants have been used for a long time in the treatment of many diseases. Dietary and
medicinal herbs contain many natural phytochemicals such as phenolics and flavonoids, and they have
antioxidant, anti-inflammatory, anti-proliferative and apoptotic effects. There is a huge interest to
uncover the valuable compounds and medicinal characteristic of herbs. The genus Paracaryum (DC)
Boiss. belongs to Boraginaceae family. In this study, the essential oil composition, antioxidant, anti-
proliferative and apoptotic effects of Paracaryum bingoelianum were investigated. Paracaryum
bingoelianum was collected in 2014, in Bingol, Turkey. The essential oil of P. bingoelianum was
obtained by hydrodistillation method and chemical composition of plant was analyzed by HS-
SPME/GC-MS. The antioxidant capacity of essential oil from P. bingoelianum was determined by
different in vitro assays (DPPH radical scavenging activity, reducing power and ferric thiocyanate
methods). BHA, BHT, vit.E and vit. C were used to compare the antioxidant assay results. Anti-
proliferative activity was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium
bromide) assay on human colorectal adenocarcinoma cell line (HT-29). Cell death detection Elisa
assay was used to detect the apoptotic effect. Thirty six compounds were totally identified, which
representing 93.38% of the oil. Among them 6,10,14-trimethyl-2-pentadecanone (17.2%), eucalyptol
(9.53%) and trans-2-hexanal (8.94%) were the major compounds. At highest concentration the DPPH
radical scavenving activity of essential oil (EO) was close to vit. C (vit. E > vit. C > EO). Essential oil
showed lower reducing power than BHT and BHA. Ferric thiocyanate assays result was following
order; BHT BHA > EO. Essential oil of P. bingoelianum reduced HT-29 cell proliferation and
induced apoptosis. The inhibition of viability of HT-29 colorectal adenocarcinoma cell line treated
with 600 g/ml EO was 56.7%. IC50 value was determined as 473.2 g/ml. When HT-29 cells treated
with IC50 value of EO, apoptosis rate was higher 4.1 fold than untreated cells (p<0.05) The findings
clearly indicate that P. bingoelianum has a lot of chemical compounds and present antioxidant, anti-
proliferative and apoptotic activities in different experiment systems. Further in vivo explorations are
needed to illuminate this plants medicinal usage.

KEYWORDS
Essential oil, antioxidant activity, Paracaryum, cell culture, apoptosis

Corresponding Author: MEHMET KADR ERDOAN, mehmetkadirerdogan@gmail.com


1
BNGL NVERSTES
2
ATATRK NVERSTES

1768
Poster Session 14
Submission ID: 1778

EFFECT OF BRASSINOSTEROID APPLICATIONS ON SOME PLANT


PARAMETERS OF JAPANESE MINT (MENTHA ARVENSIS L.)

1 2
YALIN KAYA , ENGL KARAMAN

ABSTRACT
The Japanese mint (Mentha arvensis L.) belonging to the genus Mentha of the Lamiaceae
family is an important medicinal and aromatic plant. Mentha, represented by about 18 species in the
world, is spread with 7 species in our country. Menthol in the essential oil obtained from leaves of
Japanese mint is economically important. It is produced to obtain menthol for commercial purposes.
The aim of the study is to determine the effects of Brassinosteroids, which are defined as the new
generation of plant hormones, in the Japanese mint and to obtain yielded plants in terms of desired
traits. 24-Epirassinolide (EBL), the active form of brassinosteroids, was used in the study. For this
purpose, 5 EBL levels (0, 10-8,10-7,10-6 ve 10-5 M), including the control, were exogenously applied
to Japanese mint plants. The study was carried out under the unheated greenhouse condition of
Directorate of Atatrk Horticultural Central Research Institute in 2016 according to the completely
randomized designs with 4 replications and 10 plants in each replicate. Plant height (cm), wet-dry herb
yield (gr/plant), drug leaf yield (gr/plant) and wet-dry root weights (gr / plant) were determined from
the harvested plants. There was no significant effect of the applications on the fresh and dry root
weights. The highest values were obtained in terms of plant height (cm) from applications of 10-6 M
(59,92 cm) and 10-7 M (57,45 cm), respectively; (70,86 gr/plant), dry herb (21,79 g/plant) and drug
leaf yield (12,34 g/plant) per plant were obtained from 10-6 M EBL application.

KEYWORDS
Mentha arvensis, brassinosteroid, herb, mint

Corresponding Author: YALIN KAYA, yalcin.kaya@tarim.gov.tr


1
ATATRK BAHE KLTRLER MERKEZ ARATIRMA ENSTTS, YALOVA
2
KAHRAMANMARA ST MAM NVERSTES BYOLOJ BLM, KAHRAMANMARA

1769
Poster Session 14
Submission ID: 1782

ENCAPSULATION OF BIOACTIVE COMPOUNDS

1 2 1 1 1
BRA ACOLU , TUBA ZDAL , PERIHAN YOLCI MEROLU , CANAN ECE TAMER , UTKU OPUR

ABSTRACT
Encapsulation is a process in which a substance is coated by a wall material. Even though
encapsulation has been known as a recent method, its first application was reported sixty years ago in
the area of biotechnology to make production more efficient. Various encapsulation techniques has
been applied in food industry including spray drying, spray chilling or spray cooling, extrusion
coating, fluidized bed coating, liposome entrapment, inclusion complexation, centrifugal extrusion and
rotational suspension separation. Most critical part in encapsulation is the choice of coating material.
These materials include proteins, carbohydrates, natural and modified starches, fats, synthetic
polymers, gelatin and pectin. Encapsulation enables easy delivery of bioactive materials including
antioxidants, minerals, vitamins, and phytosterols. It also increases the stability of bioactive materials
during food processing and storage, prevents oxidation, increases bioactivity of bioactive compounds
and controls their interactions with food. Encapsulation increases the shelf life, storage capability,
nutrient value and digestion of bioactive compounds. Moreover, encapsulation enables efficient
utilization of polyphenols by suppressing unpleasant taste of its free compounds and also increases the
aroma quality of essential oils extracted from plant materials. This review focuses on new
technologies on encapsulation, comparison of advantages and disadvantages of encapsulation
methods, the benefits of encapsulation technology in bioactive compound industry and summarizes the
recent research studies on encapsulation of bioactive compounds.

KEYWORDS
encapsulation, spray drying, bioactive compounds

Corresponding Author: PERHAN YOLC MEROLU, pyomeroglu@uludag.edu.tr


1
DEPARTMENT OF FOOD ENGNEERNG, FACULTY OF AGRCULTURE, ULUDAG UNVERSTY, NLUFER,
16059, BURSA
2
DEPARTMENT OF FOOD ENGNEERNG, FACULTY OF ENGNEERNG, OKAN UNVERSTY, AKFRAT-TUZLA

1770
Poster Session 14
Submission ID: 1785

THE EFFECT OF GARLIC ON TREATMENT OF VAGINAL CANDIDA

1 1
MENEKE NAZLI AKER , NESLIHAN YILMAZ SEZER

ABSTRACT
Candida vaginosis is one of the most common infections of the female genital tract. At least
75% of women live with candida infection at least once in their life. There is a need to use broad
spectrum antifungal drugs in the treatment of fungal infections. However, most of the commonly used
antifungal drugs have limited use due to toxicity, dangerous drug interactions and side effects such as
liver damage and heart failure. In the past decade, a rare antifungal drug resistance has emerged in the
past. This situation, necessitated the use of non-drug methods. One of these methods is garlic. This
review, aims to reveal the efficacy of garlic in the treatment of vaginal Candida. The results of the
studies carried out in this regard, are listed below. Adejare et al. (2012), collected swab samples from
patients with vaginal candidiasis. They tested Garcinia kola, garlic (Alliums sativum), fluconazole and
miconazole on the isolates. The aqueous and alcoholic extracts minimum inhibitory concentration for
Candida albicans, were 200 mg/ml. They reported that the garlic used as herbal product has inhibitory
effects such as antifungal agents. But this effect, has been achieved in the case of high concentration
used. Elsom et al. (2003), used several commercial preparations of garlic, which included oil
macerates, pastes, tablets and powders, to examine for their anticandidal activity on Candida albicans
laboratory strain. Only the garlic tablet formulation exhibited good anticandidal activity, whereas all
the other preparations possessed virtually no anticandidal properties. Iwalokun et al. (2004), selected
Candida species recovered from vaginal swabs, urine, and blood samples for their study. They
investigated the antifungal activities of an aqueous garlic extract. Minimum fungicidal concentration
was found to be 14.9 at 24 hours. Study, supports the use of garlic in health products. Ekanola et al.
(2014), tested eighteen antifungal drugs, crude extracts of garlic and some plants/ chemicals, for in
vitro inhibitory potentials on Candida strains obtained from human oral, high vaginal and endocervical
swabs. Garlic, were inhibitory against 33.3-54.4% of the Candida strains. They also reported
significant in vitro inhibitory potentials of garlic on drug susceptible and resistant Candida species.
Kordi et al. (2005), tested garlic extract vaginal douche and clotrimazol vaginal cream on symptomatic
women in their randomized, controlled, clinical trial. Women were treated daily for 7 days and
assessed 1-2 week after the end of treatment. They reported statistically non-significant difference
between clinical results after treatment in both groups. Mycologic results of garlic extract vaginal
douche, were good but there was a significant difference between groups. Watson et al. (2013), in their
randomised double-blinded controlled study, used oral garlic tablets for vaginal candida during the
second half of the menstrual cycle on asymptomatic women colonised with Candida species. They
gave three garlic tablets or placebo orally, twice daily, for 14 days. As a result there was no difference
between the proportion of cases in the garlic and placebo groups, in the mean colony counts in both
groups. Farshbaf-Khalili et al. (2016); used garlic, Zataria multiflora boiss and clotrimazole vaginal
cream on women with candidal vaginitis, in their randomized, double-blinded clinical trial. The
patients used creams daily, for 7 days. In culture results, there were not significant differences between

Corresponding Author: MENEKE NAZLI AKER, menekseaker@gmail.com


1
ANKARA NVERSTES SALIK BLMLER FAKLTES

1771
groups on days 7 and 30 after treatment. The result of the study shows that the mean scores of candidal
symptoms, significantly reduced after treatment in all groups on days 7 and 30. The results of these
studies show that the garlic is effective on Candida. However, it is recommended to perform
randomized controlled trials in which the effect of garlic is assessed for using on patients.

KEYWORDS
vaginal candida, garlic, herbal treatment

1772
Poster Session 14
Submission ID: 1786

BIOLOGY OF STACHYS KURDICA VAR. KURDICA

1 2 1 1 1
A. SELUK ERTEKN , FATIH MEHMET KILIN , AHMET ONAY , REMZI EKNC , MEDET KORKUN , ZIYA
2
DUMLUPINAR

ABSTRACT
Stachys L. (Deliay) A sub-cosmopolitan broad ciner of the family Ballibagiller (Lamiaceae /
Labiatae). It has about 370 species in the world. Stachys is represented in Turkey with 92 species and
116 taxa (subtype and varietal level). Of these taxa, 53 are about 46% endemic. Stachys kurdica Boiss.
& Hohen. there is. Kurdica (Kara deliay) Turkey (Hakkari and Dicle Departments) and in Iraq is a
rare plant that grows. There is S. kurdica in this study. The detailed morphological characteristics of
kurdica were tried to be determined, the relation between nearby taxa was discussed and the
taxonomic condition was observed. Examples of the material of the workshop were collected from the
Southeastern Anatolia Region, which was gathered from the land in 2013-2014. In addition, some of
the Stachys examples in the G, HAL, JE, K, W, and WU virtual herbariums were exploited. There is
S. kurdica. Cystic to basal and glabrous and serrate-crenate, sometimes the entire leaves and verticillas
are mostly congested in a terminal head and are distinctly different from other nearby taxa, These
morphological features were compared with those of other investigators and the status of the two
varieties of S. kurdica grown in Turkey was preserved.

KEYWORDS
Stachys kurdica var, Morphological characteristic,Taxonomic status

Corresponding Author: FATH MEHMET KILIN, fmehmet206@gmail.com


1
DCLE NVERSTES
2
KAHRAMANMARA ST MAM NVERSTES

1773
Poster Session 14
Submission ID: 1788

FUNCTIONAL FOOD: DANDELION (TARAXACUM OFFICINALE L.)

1 1 2
MURAT KARAAA , SUZAN YALIN , S. SONGL YALIN

ABSTRACT
Dandelion (Taraxacum officinale L.) is a family of Asteraceae. It can be found in many places
especially in lawns and on roadsides. Dandelion is also considered to be a weed. However it can be
used as a medical herb and food. Leaves, flower and root of dandelion can be used. It contains
flavonoids including luteolin, apigenin, isoquercitrin, caffeic acid and chlorogenic acid. It also
contains terpenoids, triterpenes and sesquiterpenes. It is a rich source of beta carotene, carotenoids,
xanthophylls, chlorophyll, vitamin C, vitamin D, many of the B complex vitamins, choline, iron,
magnesium, sodium, zinc, manganese, copper, phosphors, and potassium. Roots contain torexacin,
retinol, levulin and inulin. Due to having bioactive compounds dandelion has properties of antioxidant,
anti-inflammatory, anticancer, hypoglycemic and hypolipidemic activities. Dandelion inulin is used as
prebiotics in medicine. Dandelion reduced the extent of atherosclerosis by reducing oxidative stress
and serum total cholesterol, triglcerides, low density lipoprotein cholesterol and raising serum high
density lipoprotein cholesterol. It enhanced effectively delayed the lowering of glucose in the blood
and prevented the increase in lactate and triglceride concentrations. -sitosterol and stigmasterol in
dandelion sterols have been identified as good inflammatory inhibitors. Therefore dandelion
(Taraxacum officinale L.) is considered as an important functional food due to their bioactive
compounds.

KEYWORDS
Dandelion, Taraxacum officinale L., Functional food, Bioactive compounds

Corresponding Author: SUZAN YALIN, syalcin@selcuk.edu.tr


1
SELUK UNVERSTY, FACULTY OF VETERNARY MEDCNE, DEPARTMENT OF FOOD HYGENE AND
TECHNOLOGY, KONYA, TURKEY
2
HACETTEPE UNVERSTY, FACULTY OF MEDCNE, DEPARTMENT OF PEDATRCS, ANKARA, TURKEY

1774
Poster Session 14
Submission ID: 1789

ANTIMICROBIAL EFFECTS OF ESSENTIAL OILS EXTRACTED


FROM AROMATIC AND MEDICINAL HERBS

1 1
EHRIBAN UUZ , SEVAL ANDI

D
ABSTRACT
Essential oils (EOs), called ethereal or volatile oils, are complex mixtures of low molecular

TE
weight compounds extracted from plants by steam distillation and extraction with various solvents.
Terpenoid hydrocarbons, oxygenated terpenes and sesquiterpenes are the major constituents that
provide characteristic aroma and biological properties to EOs. These aromatic oily liquids obtained
from plant flowers, buds, seeds, leaves, twigs, bark, herbs, wood, fruits and roots. Even in old times,
traditional systems of medicine prescribed EOs for a variety of health problems and used them for the
EN
purpose of food preservation, all over the world. Some EOs have antimicrobial properties such as
antibacterial, antifungal and antiviral. Besides these properties, EOs or their components also have
antimycotic, antitoxigenic, anticancer, antimutagenic, antidiabetic, antiinflammatory, antiprotozoal,
antiparasitic and insecticidal effects. These characteristics (the composition of essential oils and their
ES
antimicrobial effects) depend on the function of these compounds in plants, plant species and regional
conditions. Antimicrobial activity of several spices and essential oils has demonstrated on
Staphylococcus aureus, Vibrio parahaemolyticus, Salmonella typhimurium, Shigella dysenteria,
Escherichia coli, Escherichia coli O157:H7, Listeria monocytogenes, Bacillus cereus at levels between
0.2 and 10 l ml-1. Gram-negative organisms are slightly less susceptible than gram-positive bacteria.
PR

Also clove, pimento, cinnamon, thyme, oregano, garlic and onion were particularly inhibitory for
some food-spoilage microorganisms.

KEYWORDS
T

Essential oil, volatile oil, antimicrobial effect, terpenoid.


O
N

Corresponding Author: EHRBAN UUZ, sehribanuguz@yyu.edu.tr


1
YZNC YIL NVERSTES

1775
Poster Session 14
Submission ID: 1790

ANTIMICROBIAL ACTIVITY OF SPRAY DRIED SUMAC EXTRACT


POWDER ON ESCHERICHIA COLI O157:H7 AND SALMONELLA
TYPHIMURIUM

1 1 1
GLAH ALIKAN KO , GLTEN TIRYAKI GNDZ , SAFIYE NUR DIRIM

ABSTRACT
Sumac (Rhus coriaria L.) is used in Mediterranean and Middle East regions as a spice, sauce,
flavoring agent etc. Several studies demonstrate that sumac berries have the biological activities such
as antimicrobial, antioxidant, hypoglycemic and antidiabetic activities due to their flavone, tannin,
anthocyanin and organic acid contents. The aims of this study is are; to determine the antimicrobial
activity of a spray dried sumac extract powders (SDSEP) on E. coli O157:H7 and S. Typhimurium in
vitro conditions and to determine effect of pH and temperature on the antimicrobial activity of selected
SDSEP. For this reason, a pilot scale spray dryer (Mobile Minor Miro-Atomizer, Denmark) was used
for the production of the sumac extract powder. The inlet/outlet air temperatures were adjusted to
160/80, 180/90, and 200/100C where the outlet air temperature was controlled by regulating the feed
flow rate. The sumac berries were washed, drained, and ground with a blender and then mixed with
water (with ratio of 1:4) for 2 h at room temperature. Then the extract was filtered with crude filter
paper to obtain the sumac extract (SE). The total soluble solid content of the SE was measured to be as
3.5% and adjusted to 10, 15, 20, and 25% (w/w) with the addition of maltodextrin (MD) with a
Dextrose Equivalence (DE) of 1012. The atomization pressure and the air flow rate were kept
constant as 392 kPa and 1.54 m3/min respectively. The obtained powders were analyzed for moisture
content, water activity, pH, minimum bactericidal concentration (MBC), and minimum inhibitory
concentration (MIC). In order to determine the effect of pH and temperature on the antimicrobial
activity of SDSEP, the sumac extract powder (5%) which was produced at 160/80 C was selected.
The pH value of sumac extract powder (3.1) was adjusted to 6.5 6.8 with the addition of NaOH and
the inoculated samples were incubated at three different temperatures (4 C for 7 days, 22 C for 48
hours, and 37 C for 24 hours). Increasing the amount of MD and inlet/outlet temperatures showed a
significant effect on the moisture content and water activity of the sumac extract powders (P < 0.05).
The moisture content, water activity and pH values of SDSEP were ranged between 1.89-2.94 % (wet
basis, wb), 0.157- 0.215, and 3.133.23, respectively. Increasing the amount of MD caused a
significant increase in both MBCs and MICs of SDSEP (P < 0.05). The inlet/outlet temperatures were
not found to be significantly important on the MBCs and MICs of SDSEP (P > 0.05). The MBCs and
MICs of SDSEP ranged between 25.0 50.0 and 6.25 - 25.0 (mg/ml) against the E. coli O157:H7 and
12.5 50.0 and 3.12-12.5 (mg/ml) against the S. Typhimurium. E. coli O157:H7 was found to be more
resistant compared to S. Typhimurium. The increase of pH value of SDSEP resulted in a significant
decrease in the antimicrobial activity of SDSEP (P < 0.05). Results obtained in this study suggest that
the addition of spray dried sumac powder have the potential to improve the safety of foods such as
salads and meat products which could be a source of tested pathogens.

Corresponding Author: GLAH ALIKAN KO, gulsah.caliskan@ege.edu.tr


1
EGE NVERSTES

1776
KEYWORDS
Sumac Extract, Sumac Extract Powder, Spray Drying, Antimicrobial Activity

1777
Poster Session 14
Submission ID: 1791

INFANTS OF PARENTS OF INFANTS WITH COLIC DISORDER AND


THEIR OWN HERBAL PRODUCTS

1 1
DO. DR. EMNE GEKL , AR. GR. MURADYE ALDEM

ABSTRACT
Colic during babyhood is defined to be the uneasiness and crying jags that last more than three
weeks, more than three days a week and more than three hours a day. The studies indicate that the
frequency of babyhood colic varies between 5%-%25. Usually, colic tends to start in the second or
third weeks after birth, peak in the sixth or eighth weeks and end in the third or fourth months. The
crying jags usually start in the afternoon or in the evening and last for several hours with continuous
and loud crying. Sometimes babies also cry at night. The baby colic may arise from nutrition mistakes,
food allergy, lactose intolerance and gastrointestinal causes including reflux. On the other hand, baby
colic may arise from extra intestinal causes including neurodevelopmental or psychological factors.
Whatever the cause, baby colic is a condition that causes babies to cry and creates physiological and
psychological problems in babies and parents. Parents adopt various methods to silence colicky babies
including holding, shaking, car riding, giving a bath, warm and hot applications to stomach,
massaging, rhythmic voices or use of the vacuum cleaner voice etc. Other than these methods, parents
of colicky babies use various herbal products to reduce the colic of their babies. The herbal products
used to reduce the baby colic are generally consumed orally by the baby and the mother. There are
also various oils applied on the stomach or perianal area by the parents. Herbal Products Given Orally
to Colic Babies It is reported that the herbal teas used for babies relax the bowel smooth muscles and
palliate the baby colic (Zengin, nar, Altnkaynak 2016). The studies made with fennel, melissa,
licorice root, camomile tea and common vervain revealed that these teas were more effective than
placebo (Akam, 2004; Karabayr and Ouz 2009; Zengin, nar, Altnkaynak 2016; Arkan, Alp,
Gzm, Orbak, Karaca ifti, 2008). Herbal Products Given Orally or Prohibited to the Mother of the
Colic Baby Breastfeeding mothers are recommended to consume a diet without egg, hazelnut, peanut,
cracked wheat, legume, cabbage, radish and spicy food (Zengin, nar, Altnkaynak 2016). Examples
of herbal products recommended to mothers include herbs like aniseed and dill which include volatile
oils and anethol. Mothers are recommended to use three to four drops three times a day from the
mixture of herbal and natural oils such as fennel oil, sunflower oil, sweet almond oil, aniseed oil,
sesame oil, cumin and dill. Herbal Products That Are Externally Applied to Colicky Baby Massaging
to stomach area with fennel oil was found to be effective in eliminating the gas pains. Giving baby a
bath with the water added with lavender or lemon essential oil and massage with catmint tincture are
recommended to eliminate the colic (Akam, 2004).

KEYWORDS
Colic, baby, medicinal plant

Corresponding Author: AR. GR. MURADYE ALDEM, ikra42reyhan@gmail.com


1
NECMETTN ERBAKAN NVERSTES

1778
Poster Session 14
Submission ID: 1792

A RAPID METHOD FOR THE DETERMINATION OF CURCUMIN IN


FOOD SAMPLES

1 1 1 2
MEHMET ZTRK , ERHAN KAPLANER , MEHMET HSEYN SNGE , ADEM TURAN

ABSTRACT
Curcumin which has a wide area of usage, a yellow colored pigment and the major component
of Turmeric plant. Beside of being a strong anticancer agent, it has been reported to possess important
biological activities like anti-inflammatory.1,2. It becomes essential to find a rapid method for the
determination of curcumin because it has a great importance for human health as well as for the food
industry. In this study, curcumin was extracted from turmeric plant via soxhlet with extraction yield of
5.9%. Later we tried to create a metal complex of curcumin to make it easier to analyze curcumin in a
food matrix. After selecting appropriate metals to give a stable complex with curcumin, UV spectrums
were recorded while metal concentration was kept at 0.1 M with varying curcumin concentrations.
According to results, it was observed that Zr2+-curcumin complex red-shifted the maximum
absorption wavelength. Validation and recovery studies were also performed. The performance of the
method was measured for yogurt sample and 97.9% recovery was yielded. Acknowledgements: This
study is supported by the Mugla Stk Koman University Research Fund with the project number
15/213. Kaynaklar (1) Pret-Almeida, L.; Cherubino, A. P. F.; Alves, R. J.; Dufoss, L.; Glria, M. B.
A. Separation and determination of the physico-chemical characteristics of curcumin,
demethoxycurcumin and bisdemethoxycurcumin. Food Res. Int. 2005, 38 (89), 10391044. (2)
Zhang, J.; Jinnai, S.; Ikeda, R.; Wada, M.; Hayashida, S.; Nakashima, K. A simple HPLC-fluorescence
method for quantitation of curcuminoids and its application to turmeric products. Anal. Sci. 2009, 25
(3), 385388.

KEYWORDS
Turmeric (Curcuma longa), Curcumin, metod validation

Corresponding Author: MEHMET ZTRK, mehmetozturk@mu.edu.tr


1
MUGLA STK KOCMAN UNVERSTY
2
YEENLER KMYA, KKEKMECE, STANBUL

1779
Poster Session 14
Submission ID: 1794

EFFECTS OF AROMATHERAPY ON DYSMENORRHEA

1 1
NESLHAN YILMAZ SEZER , MENEKE NAZLI AKER

ABSTRACT
Dysmenorrhea is excessive pain experienced during menstruation. Classically there are two
types: primary dysmenorrhea and secondary dysmenorrhea. The main symptom of dysmenorrhea is
pain concentrated in the lower abdomen or pelvis. It may radiate to the thighs and lower back.
Symptoms often co-occurring with menstrual pain include nausea and vomiting, diarrhea or
constipation, headache, disorientation, hypersensitivity to sound, light, smell and touch, fainting and
fatigue. Dysmenorrhoea is the most common gynecological complaint that occurs in women and daily
life activities, business efficiency. For this reason, treatment is important. Therapy on dysmenorrhea is
multifaceted and depends on the severity of the pain and the response the patient gives to the pain. It is
possible to use pharmacologic and nonpharmacologic methods in treatment. Non-steroidal anti-
inflammatory drugs and hormonal birth control can be used in pharmacologic treatment. In
nonpharmacologic methods, local hot application, good nutrition, regular exercise, regular and
adequate sleep, acupuncture, acupressure, herbal therapies and aromatherapy can be used.
Aromatherapy is the use of high concentrations of essential oils and odors distilled from plants to use
their therapeutic properties. Aromatic essential oils are used by way of inhalation, compression, and
massage. Studies have shown that aromatherapy is applied more by massage in the treatment of
dysmenorrhea. At the same time, there are studies on inhalation. Massage is mostly applied to the
lower abdomen and most of the lavender and rose oil are used. Apart from these, essential oils used in
massage are cinnamon, clove, rose, and lavender, clary sage, marjoram, ginger, and geranium.
Lavender and rose oil alone are used in base oils, while other essential oils are often used as a mixture.
The most preferred base oils are sesame and almond oil. In a study with 80 primary dysmenorrhea
student, Bakhtshirin et al. (2015) a significant decrease in VAS score after lavender massage was
detected in comparison with placebo massage. A randomized controlled trial Shahr et. al. (2015),
subjects were randomly divided into three groups: massage group with rose oil, who applied self-
massage with Rose damascene; a placebo group who performed self-massage with unscented almond
oil and a no treatment control group who applied just self-massage. In the second cycle, the menstrual
pain was significantly lower in the rose oil group than in the other two groups after intervention. Hur
et. al. (2011) investigated the alleviating effects of aromatherapy massage and acetaminophen on
menstrual pain in Korean high school girls. The cases were divided into two groups: the aromatherapy
massage group and the acetaminophen group. The abdomen was massaged once using clary sage,
marjoram, cinnamon, ginger, and geranium in a base of almond oil. The reduction of menstrual pain
was significantly higher in the aromatherapy group than in the acetaminophen group. In the use of
aromatherapy by inhalation, rose oil and lavender are found. Uysal et all (2016) evaluate the effects of
rose essential oil on primary dysmenorrhea. One hundred patients were randomly divided into two
groups; Group D received diclofenac sodium (75 mg/im) and Group A administered diclofenac
sodium with aromatherapy (2% rose essential oil). The 30th min mean VAS value in Group D was

Corresponding Author: NESLHAN YILMAZ SEZER, ylmzneslihan@hotmail.com


1
ANKARA NVERSTES SALIK BLMLER FAKLTES

1780
higher than in Group A. A randomized clinical trial Raisi Dehkordi et.al. (2014) subjects were
randomized into two groups: experimental who inhaled lavender based on sesame oil, and placebo
who inhaled sesame oil only. The symptoms of dysmenorrhea were significantly lowered in the
lavender group compared to the placebo group. Studies have shown that aromatherapy was effective in
alleviating dysmenorrhea symptoms. Aromatherapy can be provided as a nonpharmacological pain
relief measure and as a part of nursing care given to girls suffering from dysmenorrhea.

KEYWORDS
Dysmenorrhea, aromatherapy, aromatic essential oil

1781
Poster Session 14
Submission ID: 1795

PEST SPECIES DETERMINED ON ANATOLIAN SAGE, SALVIA


FRUTICOSA MILL. (LAMIACEAE)

1
N. ZLAL ELEKCOLU

ABSTRACT
Among the sage species that grow in Turkey, the most commonly collected one which is used
both for domestic consumption and exportation is the Anatolian sage, Salvia fruticosa Mill,
(Lamiaceae), This study was carried out in order to determine the pest species in S. fruticosa cultivated
in the research and application parcel of .. Karaisal Vocational School (Karaisal / Adana), Plant
and Animal Production Department in 2015-2016. As a result, pest species belonging to 3 classes
(Gastropoda, Insecta, Arachnida), 8 orders and 16 families were determined. It has been determined
that species belonging to the family Cicadellidae (Hemiptera) are the most common species and cause
staining, curling and deformation by damaging the leaves. Since this situation will lead to loss of
yield, and because it is thought that they have adverse effects on the active compounds in the parts
used as drugs, it was concluded that they were the important species to focus on.

KEYWORDS
Medicinal plant, aromatic plant, Salvia fruticosa, Cicadellidae

Corresponding Author: N. ZLAL ELEKCOLU, nelekcioglu@cu.edu.tr


1
UKUROVA NVERSTES, KARASALI MESLEK YKSEKOKULU

1782
Poster Session 14
Submission ID: 1796

DETERMINATION OF ANTIPROLIFERATIVE ACTIVITIES OF


SATUREJA BOISSIERI EXTRACTS AGAINST PC3 (HUMAN
PROSTATE CANCER) CELLS

1 1 1 2
MUHAMMED ALTUN , ALI RIZA TFEKI , BRAHIM DEMIRTA , LTFI BEHET

ABSTRACT
Cancer is one of the major diseases in today's world. According to the World Health
Organization, if no precautions are taken against cancer, 26 million people worldwide will have cancer
until 2030. Plants synthesize many primary and secondary metabolites called organic compounds.
Primary metabolites take part in important events such as photosynthesis, respiration, growth and
development. Secondary metabolites are chemotaxonomically important natural products studied
widely in last decades. Satureja boissieri Hausskn. ex Boiss. (Lamiaceae) is known as Catli/Kekik in
Turkey. The aim of this study is to extract the active components from the of Satureja boissieri and to
investigate the anticancer effects of these components against PC3 (human prostate cancer cell) cell
lines.

KEYWORDS
Satureja boissieri, anticancer activity, PC3, extraction

Corresponding Author: MUHAMMED ALTUN, muhammedaltun@karatekin.edu.tr


1
ANKIRI KARATEKN NVERSTES, FEN FAKLTES, KMYA BLM, ANKIRI
2
BNGL NVERSTES, FEN EDEBYAT FAKLTES, BYOLOJ BLM, BNGL

1783
Poster Session 14
Submission ID: 1798

SUPERCRTICAL CO2 EXTRACTION OF CARDAMOM OIL AND


DETERMINATION OF VOLATILE COMPOUNDS OF CARDAMOM
OIL BY USING GC-MS SYSYTEM

1 2 1 1
MIT ALTUNTA , CEREN DAKAYA DIKMEN , EVREN DEMIRCAN , KADRIYE NUR KASAPOLU , BERAAT
1
ZELIK

D
ABSTRACT

TE
Cardamom is the dried fruit of the tall perennial herbaceous plant, Elettaria Cardamomum
Maton and it is also known as Kakule in Turkey. It is a member of the ginger family, and features a
spicy, refreshing aroma that can be uplifting. The cardamom seeds have a warm, slightly pungent and

EN
highly aromatic flavor. Therefore, it is used as a spice in some meat products. In Turkey, consumption
of coffee containing cardamom seed is common in southeast regions. The basic cardamom aroma
produced by a combination of the major components, 1,8-cineole and -terpinyl acetate. Cardamom is
also popular because of its profound effects on the respiratory system due to its high 1, 8-cineole
content, which promotes clear breathing and respiratory health. The chemical composition of
ES
cardamom varies considerably with variety, region and age of the product. Cardamom essential oil
conventionally produced by steam distillation from the seeds after they have undergone a long drying
process to get the best levels of chemical constituents possible. The content of volatile oil in the seeds
is strongly depending on storage conditions, but may be as high as 8%. This study was carried out to
extract Cardamom volatile oil and determine its volatile compounds by using GC-MS system.
PR

Cardamom seeds were extracted with supercritical carbon dioxide extraction method. Then GCMS
analysis was performed for determination of volatile compounds of Cardamom oil. According to data
obtained from GC-MS, the major volatile compounds of Cardamom oil were 1.8 cineole, sabinene, -
pienene and -terpynenil-acetate. The volatile oil contains about 1.52% -thujene, 10.27% -pinene,
1.65% -pinene, 18.86% sabinene, 4.22% -myrcene, 1.11% p-cymene, 46.8% 1,8-cineole, 2.96%
linalool, 0.48% terpinen-4-o1, 0.73% -terpineol, 2.98% linalyl acetate, 7.31% -terpinenyl acetate,
T

0.55% trans-sabinene hydrate and 0.63% trans-sabinene hydrate acetate.


O

KEYWORDS
N

cardamom oil, volatile compounds, supercritic CO2 extraction

Corresponding Author: MT ALTUNTA, ualtuntas@itu.edu.tr


1
STANBUL TEKNK NVERSTES

1784
Poster Session 14
Submission ID: 1799

RESEARCH ON THE POSSBLTY OF LAVANDER CULTVATON


N THE CTY OF DYARBAKIR

1 1 2 3 2
MEDET KORKUN , REMZ EKNC , FATH MEHMET KILIN , AHMET ONAY , ADEM BARDAK

ABSTRACT
The city of Diyarbakr lies in the Southeast Anatolia region. It is divided into different
subclimatic regions by its climate, topographic structure and soil structure. This situation causes
differences in flora and fauna structure in the city and helps it to have a richer structure. Lavender,
which can grow up within the city border in our city Diyarbakr, is a plant of perfumery, cosmetics and
medicine that is cultivated around the world because of its high ratio and high quality evaporating oil.
The aim of our research is to identify this endemic plant that exists or can exist in the flora and to put
forth the medical and aromatic properties of different kinds of this plant that is grown up. The highly
growing interest in lavender farming in recent years increased the importance of scientific research
done on advanced cultivation methods of this plant and many improvements have been performed.
Especially, in the species that have a problem in replication with seeding, research in micro
replications with tissue cultures has gained a lot of value. If the types of dry farming plant lavender,
which has adapted extremely well to Diyarbakr regions nonirrigated, arid and inclined fields,
producing evaporating oil that can be marketed worldwide can be identified and after that if these
identified types seedlings could be quickly replicated and distributed to producers, lavender will be a
huge gain for both Diyarbakr and Turkish economy. In Turkey, lavender cultivation has been done
traditionally for 40 years in a few cities to produce evaporating oil. Research since the end of March
2017 has been performed with the aim of bringing forth new types of lavender and lavandin that
possesses high flower efficiency and evaporating oil quality for the region and that have high
adaptation abilities; researching possibilities of replication with steel in in vivo conditions; realizing
numerous healthy seedling production by in vitro micro replication using tissue culture techniques. In
the research, Raya, Munsted, Silver and Vera types belonging to Lavandula Angustifolia
species are being planned as a material to be replicated with rooting in farm and greenhouse trials. We
will be working towards the replication of the seeds of types that will naturally be raised in our citys
flora by taking them into culture for intense production and towards the identification of medical and
aromatic properties (fenolics and flavonoids), contents of evaporating and nonevaporating fatty acids
and terpenoids. In this respect, trial productions of lavender cultivation will be performed. As a result
of the aim of this research; since lavender farming is done economically in only a few cities, bringing
forth lavender and lavandin types that have high flower efficiency and high evaporating oil quality
(having high ratios of acetate and low ratios of camphor) for the region and that have high adapatation
ability; identifying these types farming and technological properties; researching the replication with
in vivo steel possibilities of lavender types that has limited or sometimes no production possibility
with seeding; and realizing the production of numerous healthy seedlings by in vitro micro replications
from ripe lavender plants by making use of tissue culture techniques.

Corresponding Author: MEDET KORKUN, medet.korkun@dicle.edu.tr


1
DCLE NVERSTES
2
KAHRAMANMARA ST MAM NVERSTES
3
DCLE NVERSTES

1785
KEYWORDS
Lavender, medical and aromatic plants

1786
Poster Session 14
Submission ID: 1800

INVESTIGATION OF PREPARING A PROPOLIS CREAM WITH


OLIVE OIL

1 2 1
SELAHATTIN SERN , MUSTAFA KEMAL SANGN , GRAY KILINEKER

ABSTRACT
Propolis is a resinous material collected by honeybees from the flowers and trees [1]. Propolis
is characterized by a series of biological properties, such as antibacterial, antiviral, antifungal, anti-
inflammatory and antioxidant [1]. The applications are showed that propolis extracts are used to
prevent and treat oral inflammations, cold syndrome and dermatological preparations [3]. The
chemical composition of propolis is known to be very complex and influenced by the geographic
origin [3]. In propolis active compounds are polyphenols, including phenolic acids and flavonoids
[2,3]. Raw propolis cannot be used because of its useless materials, therefore it must be purified by
solvent extraction to remove that unwanted materials and preserve the active polyphenolic fraction
[2,3]. The extraction techniques used for the chemical analysis of raw propolis at room temperature is
heat reflux extraction have been widely applied [2,4]. In this study, raw propolis is collected from the
beekeeper from Pozant plateau district. Before extraction raw propolis chunks into smaller pieces and
stored in freezer for 1-2 days until it is hard. Then it is grinded to a powder. For the extraction, 5
grams of propolis powder in 100mL ethylalcohol (70%) is subjected 30min ultrasound-assisted
extraction. It is filtered and evaporated under vacuum at 50C. The extract is mixed with olive oil and
some additives such as glycerin, thickening agent and emulsifier. The formulation of the cream and
analysis detailed for creating a cream mark. References: [1] J.M. Sforcin, V. Bankova, Propolis: is
there a potential for the development of new drugs? J. Ethnopharmacol. 133 (2011) 253260. [2] A.M.
Gmez-Caravaca, M. Gmez-Romero, D. Arrez-Romn, A. Segura-Carretero, A. Fernndez-
Gutirrez, Advances in the analysis of phenolic compounds in products derived from bees, J. Pharm.
Biomed. Anal. 41 (2006) 12201234. [3] Y. Xu, L. Luo, B. Chen, Y. Fu, Recent developments of
chemical components in propolis, Front. Biol. China 4 (2009) 385391. [4] F. Pellati, G. Orlandini, D.
Pinetti, S. Benvenuti, HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of
propolis extracts, J. Pharm. Biomed. Anal. 55 (2011) 934948.

KEYWORDS
Propolis, olive oil, ultrasound-assisted extraction, cream

Corresponding Author: MUSTAFA KEMAL SANGN, ksangun@gmail.com


1
UKUROVA UNVERSTY, FACULTY OF SCENCE&ARTS, CHEMSTRY DEPARTMENT, 01110, ADANA
2
MUSTAFA KEMAL UNVERSTY, FACULTY OF SCENCE&ARTS, CHEMSTRY DEPARTMENT, 31024, HATAY

1787
Poster Session 14
Submission ID: 1801

THE ETHNOBOTANICAL FEATURES OF STRATONIKEIA ANCIENT


CITY PLANTS

1 1 1
YASEMIN AKKAVAK , UYGAR SARPKAYA , OLCAY DEN

ABSTRACT
In this study, the ethnobotanical properties of plants species distributed in Stratonikeia ancient
city and its vicinity (Eskihisar village in Mula)' between 2010-2012. A total of 318 plant taxon were
evaluated their ethnobotanical properties. Two taxon are belongs to Gymnospermae and 316 of them
belongs to Angiospermae. 265 species of belongs to Dicotyledonopsida and 51 species of
monocotyledonopsida. The ethnobotanical usages of these plants, local names, used parts, etc. were
reported. In generally, we observed these plants are used for traditional medicine, food, handcrafts,
fuel, beliefs.

KEYWORDS
Ethnobotany, Stratonikeia Ancient City Mula

Corresponding Author: YASEMN AKKAVAK, yasemin0331@gmail.com


1
PAMUKKALE NVERSTES FEN EDEBYAT FAKLTES BYOLOJ BLM

1788
Poster Session 14
Submission ID: 1803

DETERMINATION OF PHENOLIC COMPOUNDS IN CHESTNUT


POLLEN AND ITS ANTIOXIDANT EFFECT ON DNA OXIDATION
SYSTEM

1 1 1 2
BRA KARKAR , EFTAL ALP DORKEN , SALIHA AHIN , M.ERTAN GNE

ABSTRACT
Bee-pollen has long been used as a dietary supplement and also as an additive in cosmetics,
food and medicine. It is composed of nutritionally essential substances such as carbohydrates,
proteins, amino acids, lipids, vitamins, mineral substances and trace elements. Also pollen has been a
rich source of flavonoid glycosides, which contains beneficial antioxidant, anti-inflammatory, anti-
allergen, antiulcer, antibiotic and anti-carcinogenic properties. Bee pollen is used to cure conditions
such as colds, flu, ulcers, premature aging, anemia, colitis, allergic reactions and enteritis. It is also is
also used in the field of cosmetics for its contribution of vitamins to cold creams. Pollen is known to
be supportive in the treatment of cancer. Antioxidant compounds can increase product shelf life by
retarding the process of lipid peroxidation, which is one of the major reasons for deterioration of foods
during processing and storage. Phytochemicals, such as phenolic compounds, are considered
beneficial for human health because they decrease the risk of degenerative diseases by reducing
oxidative stress and inhibiting macromolecular oxidation such as DNA. They have been shown to
possess free radical scavenging and metal chelating activity in addition to their anticarcinogenic
properties. The aim of this study was to determine antioxidant phenolic compounds in chestnut pollen
and its antioxidant effect on DNA oxidation system. The chestnut pollen was extracted with organic
solvent such as ethanol. The phenolic compounds in ethanolic extract of chestnut pollen were
determined by high-performance liquid chromatography diode-array detector (HPLC-DAD). Syringic
acid, hyperoside, kaempferol, isorhamnetin, pinocembrin, chrysin and galangin were detected in
chestnut pollen. And also the antioxidant property of pollen extract was evaluated according to Folin-
Ciocalteu and a chromium reducing antioxidant capacity (CHROMAC) methods. The CHROMAC is
a novel method as a spectrophotometric total antioxidant capacity assay based on the reaction of
excessive Cr(VI) with phenolic compounds and formation of coloured complex with remaining Cr(VI)
and diphenylcarbazide as described by our previous study. Antioxidant ability of chetsnut pollen was
examined to investigate the prevention of oxidation of calf thymus DNA in Fenton reaction medium. It
was understood that the chestnut pollen prevented DNA oxidation damage of 10% under oxidative
environment. Consequently, the extract of chestnut pollen showed a significant antioxidant activity for
the examination of DNA damage prevention.

KEYWORDS
chesnut pollen, DNA oxidation, Fenton, HPLC, CHROMAC

Corresponding Author: SALHA AHN, salihabilgi@uludag.edu.tr


1
UNVERSTY OF ULUDAG, FACULTY OF SCENCE AND ARTS, DEPARTMENT OF CHEMSTRY
2
UNVERSTY OF ULUDAG, VOCATONAL SCHOOL OF TECHNCAL SCENCES

1789
Poster Session 14
Submission ID: 1804

BLACK TEA WASTE NOT MUCH DIFFERENT FROM BLACK TEA

1 1 2 1
MURAT KK , OZAN EMRE EYUPOLU , AYA AKTA KARAELIK , SEMRA ALKAN TRKUAR , UUR
3
KARDIL

ABSTRACT
The black tea is produced from the leaves of Camelia sinensis plant. The waste of the
production process is discarded without any proper use. Black tea and black tea waste were studied in
order to determine similarity or difference both in composition and antioxidant activities. Three widely
used spectrophotometric antioxidant assays revealed that black tea waste retains much of the activity
of black tea, with 39% of DPPH scavenging activity, 76% of FRAP activity, and 84% of total
phenolics content. On-line RP-HPLC-DAD-FRAP composition and antioxidant analyses showed that
black tea waste has very similar compositional and antioxidant profile with black tea, the waste having
more than 60% of the components of black tea. The chromatograms illustrate the mainly overlapping
peaks in black tea and waste samples, and the FRAP chromatogram was same in the two samples.
Inactive caffeine and highly active antioxidant gallic acid were the two major components in both tea
and tea waste. Thus, besides its use as a source of caffeine and catechins, black tea waste can well be
used for tea preparation, probably in tea bag form or as extract.

KEYWORDS
black tea, tea waste, on-line HPLC-FRAP, DPPH, total phenolics

Corresponding Author: MURAT KK, mkucuk@ktu.edu.tr


1
KARADENZ TEKNK NVERSTES
2
GRESUN NVERSTES
3
GMHANE NVERSTES

1790
Poster Session 14
Submission ID: 1805

PRODUCTION OF FOOD COLORING FROM SAFFRON AND


SAFFLOWER

1 2
OSMAN SADI , YKSEL BAYRAM

ABSTRACT
Color materials are widely used in the food industry because they are a very important feature
that determines the preference of food. In recent years, the adverse effects of some synthetic food
colors on health have increased and in some countries, the prohibition of these colors has led to an
increment in the tendency towards natural resources and studies on the use of the colors obtained from
these sources have become increasingly important. In this study, the characteristics of saffron and
safflower to be used for natural food dyeing will be emphasized. Saffron (Crocus sativus) is an onion
culture plant of the family of Iridaceae that grows 20-30 cm in length, with purple flowers opening in
the autumn. The flower harvest starts from October to the middle of November. In the world, saffron
is mostly grown in Spain, France, Italy and Iran. While the production of saffron in Turkey is only
done in Safranbolu province of Karabk until now, trial production has also started in Tokat,
Kastamonu, Denizli and anlurfa. Safflower (Carthamus tinctorius) is a grassy herbaceous Carthamus
family from the Asteraceae family. There are types that are one or two years old. Safflower, which can
reach 60 to 70 cm in length, opens yellow, red and orange flowers according to cesidium in July-
September. The main land of this plant is Arabia. Safflower, which does not grow all over our country,
is usually grown in Trakya, Isparta, Burdur and Eskiehir regions. Saffron traditionally has been used
for many years as an alternative to traditional culinary arts because of its positive effects on the health
and the kitchen, mostly as a color, aroma and flavoring spice. Therefore, many studies have been
carried out on the bioactive properties of this flower. Antioxidant, antitumor, antidepressant, appetite
regulator, enhances learning capacity and improves memory. Safflower is a plant with a very
important place in the industry. It is preferred as dye material in food, cosmetics and textile products
because of the dye giving feature of the flower petals. Safflower can be used for medicinal purposes in
the treatment of certain diseases and also as tea. Saffron has important bioactive components. The
main ones are; crocin, crocetin, picrocrosin and safranal. Two basic carotenoids, crocin and crocetin
are responsible for the color. The dried stalk of Crocus Sativus is reddish yellow due to its cross and
crossover. Crosin (24-27%) which is very well soluble in water and gives a color that is saffron has a
bright yellow color. The taste of saffron comes from the picrocrosin, the second most commonly
found ingredient, which makes up about 1% to 13% of the dry matter of the saffron. Picrocrocin is a
compound that gives a bitter taste to safran. It adds flavor to food especially when used in meals.
Safranal is the essential oil which is mainly responsible for the flavor of the saffron. At the same time
more than 160 additional volatile components have been identified. Carotenoid derivatives of saffron,
in particular crocin; due to its nutritive properties, it finds wide application in the food, cosmetic and
nutraceutical industries. There are two color substances of safflower, safflower yellow (safflomin A,
safflomin B) and safflower red (carthamin). The toxicity of synthetic food coloring has been a topic of

Corresponding Author: YKSEL BAYRAM, ybayram@pau.edu.tr


1
YILDIZ TEKNK NVERSTES
2
PAMUKKALE NVERSTES

1791
controversy in recent times, increasing the need for healthy food stains to be obtained from natural
sources. Because of this, it is very important to use coloring materials which can be extracted from
such flowers, which have been used as spices for many years and which have positive health effects on
their content, as natural food dyes.

KEYWORDS
Saffron, Crocus sativus L., Safflower, Carthamus tinctorius, Food colorant

1792
Poster Session 14
Submission ID: 1806

THE NUTRIENTS OF FORMULA MILK IN TERMS OF MEETING


THE NEEDS OF AN INDIVIDUAL

1 2 1 1
KENAN SINAN DAYISOYLU , BETL DEMR , BETUL KAHVEC , MOHAMMED ZAKIR BRAHM

ABSTRACT
Kenan Sinan DAYISOYLU, Betl DEMR, Betul KAHVEC, Mohammed Zakir BRAHM
Kahramanmara Stimam University, Faculty of Engineering and Arthitecture, Department of Food
Engineering, Avar Campus, 46100, Kahramanmara, Turkey E-mail: kesiday@ksu.edu.tr Adequate
and balanced nutrition is one of the most important determinant of human health, growth and
development, more especially in babies. Cow milk is not recommended for newborn infant who
cannot breastfeed because it causes iron deficiency in some of the newborn babies. In this period,
some standardized dairy products are recommended and preferred as an alternative to breast milk such
as; baby milk, baby formula, the bottle milk, formula milk, newborn milk e.t.c. In Turkey, the
specifications of these products, which are regarded as approximate formulas for mother`s milk, are
determined and issued to the markets with the permission of the Ministry of Food Agriculture and
Livestock. In this study, the contents of breast milk and formula milk were compared and it also
includes the daily energy and nutritional requirement needs of baby`s between 0-6 up to 07-12 month.
In this study, the contents of breast milk and formula milk were compared and it also includes the
daily energy and nutritional requirement needs of baby`s between 0-6 up to 07-12 month.
Furthermore, vitamin and mineral contents of cow milk and soya protein isolates, which are used in
single and mixed form, have also been studied as based on 100 kcal. In addition, according to the
written legal limits , the use of newborn milk in feeding 0-6 and 7-12 month infants is been
researched. More especially, the situation and the meeting rates of baby needs is been investigated.It
has been determined that the formula milk produced according to the reference value given in the
Communiqu does not adequately meet the needs of vitamin A, D, E, Niacin, Folate, Choline, Iodine,
Magnesium, Copper and Selenium requirements in the 0-6 month period. It has been found that the
formula milk produced according to the base reference value compared to the reference values given
in the Communiqu does not adequately meet the needs of protein, vitamins A, D, E, Niacin, B6,
Folate, Choline, Phosphorus, Iron, Iodine, Magnesium, Manganese and Selenium in 7-12 month
period. The formula milk produced according to the ceiling reference values found in the
Communiqu is found to meet all the needs in 0-6 month period and failed to meet the adequate iron
needed during the 7-12 month period.

KEYWORDS
Formula milk, newborn milk, baby milk, baby food

Corresponding Author: KENAN SNAN DAYISOYLU, kesiday@ksu.edu.tr


1
KAHRAMANMARA ST MAM NVERSTES, MHENDSLK-MMARLIK FAKLTES, GIDA
MHENDSL BLM
2
KAHRAMANMARA ST MAM NVERSTES, FEN BLMLER ENSTTS, GIDA MHENDSL ANA
BLM DALI

1793
Poster Session 14
Submission ID: 1807

SIMULTANEOUS DETERMINATION OF GALLIC ACID AND RUTIN


IN DIETARY TEA PRODUCTS USING RP-HPLC/DAD DETECTION

1 2
HALE SEILMI CANBAY , MAHMUT DOGANTRK

ABSTRACT
U.S. Food and Drug Administration (FDA) arranges dietary supplement products and dietary
ingredients. In 1994, The US congress changed the Federal Food Drug and Cosmetic Act [1]. Under
the Dietary Supplement Health and Education Act of 1994: Botanical products (Herbals),
complementary nutritionals (amino acids, protein rich foods, etc) and micrunutriens (vitamins,
microminerals) are all considered to be dietary supplements [2]. Dietary products are used up to
extend our diet with needed micrunutriens, herbs, protein and amino acid for ideal body function [3,4].
Different types of tea, pills and other similar products are sold to people over the internet for diet
products. Diet products can be reached very easily. High Performance Liquid Chromatography
(HPLC) system was applied to phenolic concenrations in ten dietary tea product samples in Turkey.
The gallic acid and rutin compounds of dietary tea samples were determined using methanol
extraction. The analytical HPLC system employed consisted of a Shimadzu Prominence high
performance liquid chromatograph coupled with a 20A CBM (HPLC System Controller), a diode
array detector (SPD-M20A, Tokyo, Japan), a SIL 20ACHT automatic sampler, a CTO-10ASVp
column oven and a LC20 AT pump. The analytical data were evaluated using a LC Solution data
processing system. The separation was achieved on a Agilent ZORBAX Eclipse Plus C18, 4.6 250
mm, 5 m column
glacial acetic acid (A) vs (B) methanol. The elution gradient applied at a flow rate of 0.8 mL/min was:
95% A/5% B for 3 min, 80%A/20%B in 15 min and isocratic for 2 min, 60%A/40%B in 10 min,
50%A/50%B in 10 min, 100%B in 10 min until the end of the run. Samples were dissolved in
methanol, and 100 L of this solution was injected into the column. The monitoring wavelength was
280 nm and 360 for the phenolic acids. The identification of each target compound was based on a
combination of retention time and spectral matching [5]. Table 1. Retention time (Rt), wavelength

determined for the gallic acid and rutin Compounds Rt (min) (HPLC-
Gallic acid 7.8 280 0.999 0.010 91.43 (1.03) Rutin 45.6 360 0.999 0.050 95.21 (1.11) When applied to
dietary tea products the proposed method showed good results. The calibration curves for all the
species studied showed good linear correlation coefficients (r2 0.999), independent of the method
used for sample preparation (Table 1). The quantitation limit ranged 0.010 to 0.050 g/g in dietary tea
products (Table 1).

KEYWORDS
Gallic acid, Rutin, HPLC, Dietary tea

Corresponding Author: HALE SELM CANBAY, halecanbay@mehmetakif.edu.tr


1
MEHMET AKF ERSOY NVERSTES MHENDSLK MMARLIK FAKLTES
2
MEHMET AKF ERSOY NVERSTES BLTEKMER

1794
Poster Session 14
Submission ID: 1810

DETERMINATION OF ANTIFUNGAL ACTIVITES OF


PSATHYRELLA CONDOLLEANA AND PSATHYRELLA
SPADICEOGRISEA MACROFUNGUS CULTURES

1 1 1
HEMEN GL ERASLAN , DICLE ERDODU , PERIHAN GLER

ABSTRACT
In this study, the antifungal potentials of the misella obtained from two Psathyrella
(P.condolleana and P.spadiceogrisea) collected from Krkkale province were investigated. Ethanol
and methanol extracts were prepared by taking samples from the liquid medium for determination of
antifungal activity and were absorbed by discoid diffusion method and tested againts two Fusarium
(F.oxysporium and F. proliferactum) products. As a result of the study, P.soadiceogrisea-methanol
extract was found to be most effective aganinst F.oxysporium strain in the macrofungus species used.

KEYWORDS
Antifungal activites, Psathyrella, fusarium, Krkkale

Corresponding Author: HEMEN GL ERASLAN, hemengul06@gmail.com


1
KIRIKKALE NVERSTES

1795
Poster Session 14
Submission ID: 1811

COMPARISON OF IN VITRO ANTIOXIDANT ACTIVITIES OF


DIFFERENT EXTRACTS FROM CENTAUREA VIRGATA LAM.

1 1 1
TOLGACAN AYDEMR , ALI EN , LEYLA BT

ABSTRACT
The genus Centaurea belonging to the Asteraceae family comprises about 700 species
predominantly found in the Asia, North Africa, America and Europe. This genus is represented by 34
sections and 226 species with an endemism rate of 66 % in Turkey. In traditional medicine, they are
used for fever, menstrual disorders, vaginal candidiasis ,the treatment of liver, kidney and ulcer
diseases, as antidiarrheal, stomachic, tonic, appetitive, antidiabetic, antipyretic, also as a diuretic and
expectorant. Centaurea species are characterized by the presence of acetylenes, lignans, flavonoids,
and sesquiterpene lactones as main secondary metabolites. In this study, it is aimed to investigate
antioxidant activities of hexane (CVH), chloroform (CVC), ethyl acetate (CVEA), aqueous ethanol
(CVSE) fractions of ethanol extract and ethanol extract (CVE) obtained from the aerial parts of
Centaurea virgata. The antioxidant activity of the extracts was tested by DPPH and ABTS methods
and the results were expressed as IC50 values (50% inhibitory concentration). The total phenolic
content was determined by Folin-Ciocalteu method and the results were expressed as mg of gallic acid
equivalent per g dry extract. CVEA extract had the highest DPPH radical scavenging activity with a
IC50 value of 138.7 g / mL followed by CVE (200,3 g/mL), CVC (293,2 g/mL), CVSE (610,3
g/mL) and CVH (824,8 g/mL) extracts, respectively. CVEA extract had the highest ABTS radical
scavenging activity with a IC50 value of 72,89 g / mL followed by CVC (234,6 g/mL), CVE (240,5
g/mL), CVSE (288,8 g/mL) and CVH (487,4 g/mL) extracts, respectively. When the total phenol
contents of the extracts are compared, The highest amount of phenolic content was found in CVEA
extract (51,3 mg/g). These results show that phenolic compounds may be responsible for the activity
of the CVEA extract with high antioxidant activity.

KEYWORDS
Centaurea virgata, Asteraceae, Antioxidant activity, DPPH, ABTS, Total phenolic content

Corresponding Author: AL EN, ali.sen@marmara.edu.tr


1
MARMARA UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY

1796
Poster Session 14
Submission ID: 1812

MICROENCAPSULATION AND ITS APPLICATIONS IN ESSENTIAL


OIL TECHNOLOGY

1
BANU KO

ABSTRACT
Essential oils obtained from plants are complex mixtures of natural volatile compounds. They
give plants their characteristic odors and are a common source of bioactive ingredients. Essential oils,
and their components, are gaining increasing interest in the food, agriculture, pesticide, textile,
cosmetic, and pharmaceutical industries because of their natural and safe status, wide acceptance by
consumers, and multidimensional functional properties. Furthermore, essential oils could be
considered suitable substitutions to chemical additives for use in the food industry. However, essential
oils are unstable and susceptible to degradation in the presence of oxygen, light and temperature. So
many attempts have been made to preserve essential oils through microencapsulation technology.
Microencapsulation is an effective and important tool to prepare oil-based high-quality and health-
benecial products in various industries to enhance their chemical, oxidative, and thermal stability.
The success of this technology is due to the correct choice of the wall material, the core release form
and the microencapsulation method. Emulsication, spray-drying, electrospray system, freeze-drying,
coacervation, and uidized-bed-coating are the most commonly used techniques for the
microencapsulation of essential oils. The choice of an appropriate microencapsulation technique and
wall material depends upon the end use of the product and the processing conditions involved. In this
study, some relevant microencapsulation aspects, such as the capsule, wall material,
microencapsulation methods and their use in essential oil technology will be discussed.

KEYWORDS
essential oil, microencapsulation, spray drying, freze drying

Corresponding Author: BANU KO, kocbanu@gmail.com


1
GAZANTEP NVERSTES GZEL SANATLAR FAKLTES GASTRONOM VE MUTFAK SANATLARI
BLM

1797
Poster Session 14
Submission ID: 1813

AN EDIBLE WILD AND MEDICINAL MUSHROOM OF GRESUN


PROVINCE: POPLAR MUSHROOM (PLEUROTUS OSTREATUS)

1
SANEM BULAM

ABSTRACT
The edible wild mushrooms have been used as food and food-flavouring materials in soups
and sauces for centuries, due to the their unique and subtle taste and flavor. The edible mushrooms are
also important sources of bioactive molecules and produce a wide range of secondary metabolites with
high therapeutic agents. The climate and vegetation are suitable for edible wild mushroom growing
especially in Eastern Black Sea Region of Turkey. In ebinkarahisar district of Giresun province,
Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm. (Oyster Mushroom, locally named Poplar Mushroom)
which is one of the edible wild and also internationally medicinal mushrooms naturally grows on the
rotten roots of cut poplar trees. The Poplar Mushroom has a large, oyster-like head section, with an
average diameter of 5 to 25 cm, with natural samples ranging in color from white to gray or tan to
dark-brown. They are collected and sold in the local markets by the local people between mid
September and mid December depending on the climate conditions. Pleurotus ostreatus species is
consumed as food by frying in vegetable oil or sauteing with the tomatoes and onions when it is fresh
or after freezing in the winter. In the previous studies for Pleurotus ostreatus moisture 85-87%; in 100
g dried mushroom proteins 17-42 g, lipids 0.5-5.0 g, carbohydrates 37-48 g, celluloses 11.6 g,
hemicelluloses 27.8 g, fibers 24-31 g, ash 6.1 g, minerals 4-10 g and total essential amino acids 126.7
g (excluding arginine and histidine) have been reported. Besides, the studies have previously
documented that Pleurotus ostreatus plays an important role in the treatment of many diseases due to
the its anti-inflammatory, antibiotic, antiviral, antitumor, antibacterial, antidiabetic,
antihypercholesterolic, antiarthritic, antioxidant, anticancer and immunomodulatory activities. Because
it has a large amount of medicinal compounds like water soluble protein or polysaccharides, -D
glucan, glycopeptides, lovastatin and lectin. In this review, Pleurotus ostreatus mushroom species of
Giresun province and its nutritional and therapeutic characteristics will be discussed.

KEYWORDS
Pleurotus ostreatus, Giresun, nutritional value, medicinal mushroom, therapeutic agents

Corresponding Author: SANEM BULAM, sanem.bulam@giresun.edu.tr


1
GRESUN NVERSTES

1798
Poster Session 14
Submission ID: 1815

MEDICINAL MUSHROOMS AND THEIR PHARMACOLOGICAL


EFFECTS ON HEALTH

1
SANEM BULAM

ABSTRACT
The edible wild mushrooms are consumed worldwide not only for their texture and flavor but
also for their chemical and nutritional characteristics. They have higher protein, crude fiber, vitamin,
mineral contents and contain less fat and energy. Furthermore, some mushrooms that are popular in
Asian countries like China, Japan and South Korea may also have a medicinal value. These
mushrooms are not only sources of nutrients but have also been reported as therapeutic foods which
are useful in preventing diseases such as hypertension, diabetes, hypercholesterolemia and cancer.
Their specific antitumor, antiviral, antithrombotic, antiallergic, immunomodulating, hypolipidemic,
hypoglysemic, hypotensive and anti-inflammatory effects have previously been reported. These
functional characteristics are mainly due to the presence of bioactive molecules such as dietary fiber
and in particular cellulose, chitin, beta glucans, phenolic compounds, lentinan, lovastatin, ergosterol
etc. The most popular medicinal species are Agaricus blazei (Brazilian Agaricus), Auricularia auricula
(Wood Ear), Cordyceps sinensis (Caterpillar Mushroom), Coriolus versicolor (Turkey Tail),
Flammulina velutipes (Enokitake), Ganoderma lucidum (Reishi), Grifola frondosa (Maitake),
Hericium erinaceus (Lions Mane), Inonotus obliquus (Chaga), Lentinula edodes (Shiitake), Pleurotus
ostreatus (Oyster), Polyporus umbellatus (Zhu-ling), Poria cocos (Fu-ling), Schizophyllum commune
(Split Gill) and Tremella fuciformis (White Wood Ear). The medicinal mushroom originated products
are available as nutraceuticals and dietary supplements such as mushroom mycelium powder grown on
grain and soybeans, powdered extracts in capsules or tablets and ethanolic extracts with or without
glycerin. In the last few decades the medicinal mushrooms are increasingly started to be consumed in
the Western countries among vegetarians and vegans. In this review, the most common medicinal
mushrooms from Far East to Western countries and their pharmacological potential will be
investigated.

KEYWORDS
Medicinal mushrooms, therapeutic, functional, nutraceuticals, dietary supplements

Corresponding Author: SANEM BULAM, sanem.bulam@giresun.edu.tr


1
GRESUN NVERSTES

1799
Poster Session 14
Submission ID: 1818

INVESTIGATION OF ENZYME ACTIVITY OF GARDENIN B AND


CYNARININ A.

1 1 2 2 3 3
HSEYIN AKIT , MAHFUZ ELMASTA , EVKI ADEM , ALAR GLER , NACIYE KAYHAN , EMRAH YERLIKAYA

D
ABSTRACT
Natural products have various biological activities. The secondary metabolites isolated from

TE
natural sources are used for drug discovery. Naturally occurring terpenes and flavon derivatives has a
great interest among scientist due to having broad spectrum biological activities. Many drugs act as
inhibitor or activator on some specific enzymes by binding to active site of enzyme. In this study;
Inhibitory effects of secondary metabolites isolated from different sources were tested on carbonic
anhydrase IX and chicken liver aldose reductase enzymes. CA IX is a novel target enzyme for cancer
EN
treatment [1]. Aldose reductase inhibitors uses as drug to treatment such as neuropathy, nephropathy,
retinopathy, cataract and cardiovascular diseases emerging with diabetes [2]. The inhibitory effects of
Gardenin B and Cynarinin A isolated different plant sources against two selected enzymes were
evaluated spectrophotometric method. Cynarinin A is a guaiane-type sesquiterpene lactone and found
ES
in Cynara family [3]. Isolation and characterization Cynarinin A from Centaurea polypodiifolia were
previously reported by our group [4]. Gardenin B (5-hydroxy-6,7,8,4'-tetramethoxy flavone), a
polymethoxylated flavone, was isolated from Mentha x dumetorum for the first time and characterized
by spectroscopic techniques including NMR and MS and by comprising literature data [5]. The
obtained spectroscopic data as follows: m/z [M+H]+ 359.1170, max 331, 284 nm, 1H NMR (400
PR

MHz, CDCl3) 12.60 (s, 1H, 5-OH), 7.92 (d, J = 9.0 Hz, 2H, H2 and H6), 7.06 (d, J = 9.0 Hz, 2H,
H3 and H5), 6.63 (s, 1H, H3), 4.13 (s, 3H, -OMe), 4.00 (s, 3H, -OMe), 3.97 (s, 3H, -OMe), 3.92 (s,
3H, -OMe). 13C NMR (100 MHz, CDCl3) 183.04 (C4), 164.09 (C2), 162.79 (C4), 152.97 (C5),
149.53 (C9), 145.80 (C7), 136.55 (C6), 133.00 (C8), 128.11 (C2 and C6), 123.50 (C1), 114.63 (C3
and C5), 107.00 (C10), 103.77 (C3), 62.15 (-OMe), 61.72 (-OMe), 61.14 (-OMe), 55.56 (-OMe) with
fully agreement literature. Chicken liver aldose reductase enzyme was inhibited by Gardenin B and
T

cynarinin A with IC50 values 53 and 231 M, respectively. Gardenin B and cynarinin A exhibited
inhibitory effect against human recombinant CA IX with IC50 values 86 and 346 M, respectively. 1.
O

Winum, J.Y., et al., Carbonic anhydrase IX: a new druggable target for the design of antitumor agents.
Medicinal research reviews, 2008. 28(3): p. 445-463. 2. Veeresham, C., A. Rama Rao, and K. Asres,
N

Aldose reductase inhibitors of plant origin. Phytotherapy Research, 2014. 28(3): p. 317-333. 3. Liu,
R., H. Kun-Lung, and J.-K. Liu, A new sesquiterpene lactone from the leaves of Cynara scolymus
(Compositae). Acta Botanica Yunnica, 2009. 31(4): p. 383-385. 4. elik, ., et al., Crystal structure
and computational study of 3, 4-dihydroxy-3-hydroxymethyl-9-methyl-6-methylidene-3a, 4, 5, 6, 6a,
9, 9a, 9b-octahydroazuleno [4, 5-b] furan-2, 8 (3H, 7H)-dione. Acta Crystallographica Section E:
Crystallographic Communications, 2015. 71(12): p. 1425-1428. 5. Parmar, V.S., et al., Highly
oxygenated bioactive flavones from Tamarix. Phytochemistry, 1994. 36(2): p. 507-511.

Corresponding Author: HSEYN AKT, huseyinaksit@gmail.com


1
GAZOSMANPAA NVERSTES FEN-EDEBYAT FAKLTES
2
ANKIRI KARATEKN NVERSTES FEN FAKLTES
3
SRT NVERSTES

1800
KEYWORDS
Mentha dumetorum, Centaurea polypodiifolia, Gardenin B and cynarinin A, enzyme activity

1801
Poster Session 14
Submission ID: 1819

THE MEDICINAL PLANTS SOLD IN HERBAL MARKETS IN


DENIZLI

1 1
MEHMET EK , OKAN ON

ABSTRACT
In this study, it was aimed to determine the plant species sold in herbal markets in Denizli and
used by the public for medical purposes. Within the scope of the study, 8 herbal markets in the
province center were visited, and information about the medicinal plants sold and the purposes they
were used by the public were obtained. In negotiations with herbal markets in Denizli, it is recorded
that the local names of the plants sold for medical purposes and which parts are used. Photographs of
the related parts of the plants were taken. The Latin names of well-known species have been
determined, and some of them have been sampled from their existing parts for later identification.
Some were left at the genus level. In addition, information has been obtained about which diseases the
medicinal products were used for medical purposes or for which purposes they were selected for
treatment. A list has been prepared in line with the information obtained. This list includes information
on Latin names, local names, used parts and what they are used for. As a result of the study, a total of
86 plant species belonging to 44 families and 80 genera were determined to be sold as the medicinal
plant in the herbal markets in Denizli. Of these species, 71 naturally grow in Turkey. These species are
collected from their natural habitat or cultivated. The 15 species are alien species and cultivated in
Turkey. The great majority of the species belong to the families Lamiaceae (14 species), Asteraceae
(11 species), Apiaceae (6 species) and Rosaceae (5 species), respectively.

KEYWORDS
medicinal plants, herbal market, ethnobotany, Denizli

Corresponding Author: MEHMET EK, mcicek@pau.edu.tr


1
PAMUKKALE NVERSTES, FEN EDEBYAT FAKLTES, BYOLOJ BLM, 20070 DENZL

1802
Poster Session 14
Submission ID: 1820

THE ANALYSIS OF MEDICINAL AND AROMATIC PLANTS IN


SOCIAL AND SCIENTIFIC CONTEXT

1 2 2 2
FEVZ RN , MUSTAFA KAYA , HASAN HSEYIN IFTI , TEVFIK TAYLAN ATALAY

ABSTRACT
THE ANALYSIS OF MEDICINAL AND AROMATIC PLANTS IN SOCIAL AND
SCIENTIFIC CONTEXT ABSTRACT WHO ARE WE AND WHAT OUR AIM IS The Association
of Porsuk River Philosophers (APRP) was established in 2014. The aim of establishment is to
contribute human to the awareness of the need for philosophic knowledge in personel and public
domain.The Association has been keeping holistic researches on the base of the seven classic
disciplines; Ethic, Knowledge, Science, Religion, Aesthetic, Politics, Ontology. Under the scientific
discipline, with the slogan of Returning To The Natural Life, the APRP began natural agricultural
activities in the two decares field. Primarily, in the light of natural farming principles in order to
produce Vermicompost, Medicinal and Aromatic Plants. On the other hand, due to the protocol
between Eskiehir Tepeba Municipality and APRP, some demostrations were made in the garden of
Esentepe Childrens and Art Center, local seed production and distribution have been achieved.
Throughout history, human beings have known to use the wild plants to heal the health issues. In our
age, the methods of treatment under the name of Alternative Medicine has been applying by
referring the science as a guide. Approximately, the 80% of the materials used in the manifacture of
medicines that modern medicine requires of medicinal and aromatic plants origin. Apart their use as
medicines; the medical and aromatic plants have contribution in foods, cleaning products, tooth paste
and gums, herbal teas and cosmetics. With these developments, the trade of the Medicinal and
Aromatic Plants all over the world have shown a great increase in the last 30 years. For many years,
the herbal products that are required of trade are collected from nature, but this stiuation has severely
damaged on the endemics (flora and fauna). Many medicinal and aromatic plants have faced the
danger of extinction. Also unconsciously harvesting plant have caused the invasion of dominant and
recessive plants to each others habibat. Briefly, these reasons above mentioned necessiated producing
the Medicinal and Aromatic Plants by human, cultivation, increasing yield which were cultivated,
extention of planting areas. Both Research Institutes and Universities in our country, have proper
scientific researchs. It has been pleased to see that Minister of Food, Agriculture and Livestock have
increased the project and support of Medicinal and Aromatic Plants in recent years. It is needed to
make natural agricultural applications by volunteer organisations, such as APRP, with regard to social
benefit, for making the society conscious when there is lack of public services. These kind of
activities will be tried to reach to the cultivators who have interests. It should be the responsibility of
non-governmental organisations to diseminate such activities and to transmit them to growers. In this
context, APRP planted Menta (Menthaxpiperata L.) 50 m2; Sage (Salvia officinalis) 50 m2; (Salvia
Triloba) 50 m2; Origano (Origanum vulgare) 50 m2, (Origanum onites) 50 m2, Rosemary
(Rosmarinus spicata) 50 m2 in the Sakintepe Campus, in the May of 2016. Another work has been
realised in September of 2016 by buying 20 kg saffron (Crocus sativus) from the Geit Kua

Corresponding Author: FEVZ RN, fevzisirin@hotmail.com


1
PORSUK AYI FEYLESOFLARI
2
PORSUK AYI FEYLESOFLARI DERNE

1803
Research Institute and planted in 100 m2 field which is registered as the name KARAASLAN. The
quantity of the planted Medicinal and Aromatic Plants were determined and the historical and folkloric
background of them synthesized and transferred to the cooperative organisation groups. Full paper will
be presented over these conclusions.

KEYWORDS
Medicinal plants saffron melissa

1804
Poster Session 14
Submission ID: 1822

ASPIR CULTIVATION IN DIYARBAKIR

1 1 2 3
MEDET KORKUN , HANGL ZBEY , SERAP ERKU , FATH MEHMET KILIN

ABSTRACT
Aspir (Carthamus tinctorius L.)is undoubtedly the world's most popular and sought after
medicinal and aromatic plant with all its varieties and uses. While all medicinal and aromatic plants
are examined, visual beauty of aspirin, calming, softening properties, nostalgic and distinctive smell
and magnificent landscape image reveals. Aspir is 50-100 cm length and flowers cream, white, yellow,
orange. These flowers can also be used in the art of dyeing rope and cloth for centuries in the city. The
aspiration plant is a one-year long-day oil plant with a pile root system that can go up to a depth of
about 2.5-3.0 m and can grow in average between 110-140 days. Oilseed plants have a strategic
prescription for human and animal nutrition due to oil, protein, carbohydrates, minerals and vitamins.
Adding Aspir oil to the greasy dishes, which have an important place in Diyarbakr's culinary culture,
will enrich this culture even more. The most important advantage of aspir compare to wheat and barley
agriculture is that wheat and barley agriculture can be used in the agriculture of all the tools and
equipment used from the preparation of soil to the storage of the product. In addition, drought
tolerance increases the importance of Aspir cultivation in our province. The high yield obtained in the
trial result made in nar District of our province last year has been effective in starting this work.
This will also help to develop the medical and aromatic plant-related industry, which will create
alternative sources of income that will bring innovation to the region. As a result of this work,
facilities to be established for the processing of medical and aromatic plants will contribute to the
economy of our province and country, and also increase the income level of our farmers, especially
women. Since the areas where the aspir farming will be done will become a visual feast, it will be seen
that it will be a stopping place for photographers as well as eco-tourism.

KEYWORDS
Aspirate, medical and aromatic plants

Corresponding Author: MEDET KORKUN, medet.korkunc@dicle.edu.tr


1
DCLE NVERSTES
2
HARRAN NVERSTES
3
KAHRAMANMARA ST MAM NVERSTES

1805
Poster Session 14
Submission ID: 1823

ETHNOBOTANY OF THE GENUS SCUTELLARIA L. (LAMIACEAE)


IN TURKEY

1
MEHMET EK

ABSTRACT
Scutellaria L., with about 360 species, is a subcosmopolitan genus of the family Lamiaceae.
Most of its species occur in the tropics, and in Southern Hemisphere they are present mainly in the
temperate mountains. The species of the genus Scutellaria are annual or perennial herbs or rarely
subshrub plants. Its stems are four-angled and its leaves are opposite. The genus is most easily
recognized by the shield on the calyx. As ethnobotanically, it is reported that the young leaves of S.
indica and S. baicalensis from the Asian species of the genus are cooked as vegetable and the whole
plant of S. baicalensis is used as a tea substitute (Tanaka, 1976). In addition, there is usage of S.
laterifolia (American skullcap) against neurological disorders such as epilepsy, convulsion, hysteria,
and insomnia in the New World (Millspaugh, 1974). The dried roots of S. baicalensis (Baikal
skullcap), have been used in memory enhancing in Chinese traditional medicine (Adams et al., 2007).
The genus Scutellaria consists of 39 taxa, 16 of which are endemic (41%) in Turkey. The Scutellaria
species are known as kaside in Turkish. Little is known on the usage of Scutellaria in Turkish folk
medicine. Infusion of its dried leaves is used as soothing, hemostatic and wound healing agent and
also as a tonic by people in some parts of Eastern Anatolia (zelik, Ay and ztrk, 1990). S.
orientalis was reported to be used externally and internally for constipation, hemostatic, tonic, and
wound healing purposes in Anatolian folk medicine (Baytop, 1999). According to the ethnobotanical
studies conducted in Eastern Anatolia in the recent years, there are data about the usages as wound
healing for S. orientalis subsp. bicolor, in the remedy of cancer and hemorrhoids for S. orientalis
subsp. pichleri and S. orientalis subsp. virens (Mkemre et al., 2015), and as astringent for S. orientalis
subsp. bicolor and S. orientalis pichleri (aklcolu and Trkolu, 2010). No data found about its
selling in herbal markets in Turkey.

KEYWORDS
economic botany, ethnobotany, Turkish traditional medicine, Scutellaria, Lamiaceae

Corresponding Author: MEHMET EK, mcicek@pau.edu.tr


1
PAMUKKALE NVERSTES, FEN EDEBYAT FAKLTES, BYOLOJ BLM, 20070 DENZL

1806
Poster Session 14
Submission ID: 1825

INVESTIGATION OF IN VITRO CYTOTOXIC ACTIVITY OF


LIQUIDAMBAR ORIENTALIS MILL. METHANOL AND
CHLOROFORM FRACTIONS AGAINST HT-29 AND HCT-116 CELL
LINES

1 1 2 1
SMEYRA ETINKAYA , LKNUR INAR , PEK SNTAR , H.GL DURSUN

ABSTRACT
Liquidambar orientalis Mill. is a herbaceous plant which has medicinal and cosmetic
properties in the family of Hamamelidaceae (Li vd. 1997). In the Mediterranean region, it is widely
used in phytotherapy for diseases or health problems. General area of usage are burn, wounds and cuts
(Fakir vd. 2009) In addition, ulcer, stomach ache, mouth diseases and antiseptic use are available
(Everest ve ztrk 2005). The goal of this work is to investigate the cytotoxic effects of Liquidambar
orientalis Mill methanol and chloroform fractions in HT-29 and HCT-116 (human colon carcinoma)
cell lines. The cytotoxicity of fractions was determined using XTT ((2,3-Bis-(2-Methoxy-4-Nitro-5-
Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)) assay. The HT-29 and HCT-116 cells were used to
determine cytotoxicity and chloroform and methanol fractions were investigated. As a result; IC50
values of the chloroform fraction ranged from 25 to 50 g / ml in HT-29 cells and 50 to 200 g / ml in
HCT-116 cells after treatment at different doses for 24 hours, 48 hours and 72 hours, And 250 to 500
g / ml in both HT-29 and HCT-116 cells after being treated at different doses for 24h, 48h and 72h,
respectively. According to this chlorofom fraction of Liquidambar orientalis showed strongest
cytotoxic effect while methanol fraction exhibited moderate cytotoxic effect. Further investigation is
still needed to understand their mechanisms of action and usage as antitumour agents. Reference 1) Li
J, Bogle AL, Klein AS. interspecific relationships and genetic divergence of the disjunct genus
Liquidambar (Hamamelidaceae) inferred from dNA sequences of plastid gene matK. Rhodora1997;
99:899:22940. 2) Fakir H, Korkmaz M, Gller B. Medicinal plant diversity of western mediterrenean
region in Turkey. Journal of Applied Biological Sciences 2009; 3:2:3040. 3) Everest A, Ozturk E.
Focusing on the ethnobotanical uses of plants in Mersin and Adana provinces (Turkey). J Ethnobiol
Ethnomed 2005; 1:6.

KEYWORDS
L.orientalis, cytotoxicty, XTT

Corresponding Author: SMEYRA ETNKAYA, cetinkayasumeyra@hotmail.com


1
NECMETTN ERBAKAN NVERSTES, MERAM TIP FAKLTES, TIBB BYOLOJ A.D., KONYA
3
GAZ NVERSTES, ECZACILIK FAKLTES, FARMAKOGNAZ A.D., ANKARA

1807
Poster Session 14
Submission ID: 1826

ANTIMICROBIAL ACTIVITY OF CENTAUREA URVILLEI DC.


SUBSP. ARMATA WAGENITZ

1 2 2 3 1
ALI EN , MAYRAM TYSZ , SEHER BIRTEKSZ TAN , KRAN KLTR , LEYLA BT

ABSTRACT
In this study, we reported, for the first time to our knowledge, antimicrobial activities of
hexane (CUH), chloroform (CUC), ethyl acetate (CUEA), aqueous ethanol (CUSE) fractions of
ethanol extract and ethanol extract (CUE) obtained from the whole plant of Centaurea urvillei subsp.
armata. Antimicrobial activity against Staphylococcus aureus ATCC 29213, Staphylococcus
epidermidis ATCC 12228, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212,
Klebsiella pneumoniae ATCC 4352, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC
14153, Candida albicans ATCC 10231, Candida tropicalis ATCC 750 and Candida parapsilosis ATCC
22019 were determined by the microbroth dilutions technique using the Clinical Laboratory Standards
Institute (CLSI) recommendations. CUC, CUE and CUH extracts showed moderate antifungal activity
against Candida albicans with MIC values of 312, 625 and 625 g/mL, respectively. All extracts
exhibited moderate antimicrobial activity against Candida parapsilosis (MIC: 312-625 g/mL). CUH
extract showed a strong antimicrobial effect against Candida tropicalis with a MIC value of 39 g/mL,
while CUE and CUC extracts had moderate activity with MIC values of 312 g / mL. The CUC
extract showed moderate antibacterial activity against Staphylococcus aureus, Staphylococcus
epidermidis and Escherichia coli with MIC values of 625 g / mL. The CUE extract exhibited poor
antibacterial activity against Staphylococcus aureus with a MIC value of 1250 g/mL These results
indicate that the extracts are particularly active against Candida species. Also, CUH extract which has
a very strong activity against Candida tropicalis is good candidate for further bioactivity-guided
fractionation in the search for new active antifungal compounds.

KEYWORDS
Centaurea urvillei subsp. armata, Asteraceae, Antimicrobial activity, MIC

Corresponding Author: AL EN, ali.sen@marmara.edu.tr


1
MARMARA UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY
2
ISTANBUL UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL MCROBOLOGY
3
ISTANBUL UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL BOTANY

1808
Poster Session 14
Submission ID: 1830

ANTIPROLIFERATIVE AND RADICAL SCAVENGING EFFECTS OF


SOME NATURAL PHENOLIC COMPOUNDS

1 2 2
FEYZA OKE-ALTUNTAS , AYSE AHIN YALIOLU , BRAHIM DEMIRTA

ABSTRACT
Phenolic compounds, commonly known as polyphenols, are present in plants and classified as
simple phenolic acids (hydroxybenzoic and hydroxycinnamic acids) and flavonoids. They affect
sensory properties of foods, such as flavor, astringency, aroma, and color. Research on phenolic
compounds is carried out because of their biological and pharmacological properties. The aim of the
study was to investigate the antiproliferative and radical scavenging effects of some plant origin
phenolic compounds. The radical scavenging effects of these compounds were studied by DPPH
assay. DPPH scavenging activity of the tested compounds decreased in the order of gallic acid >
chlorogenic acid > apigenin-7-glucoside > hesperidin > 4-hydroxybenzoic acid. Also, in this study, 4-
hydroxybenzoic acid (4-HBA), gallic acid (GA), and apigenin-7-glucoside (A-7-G) were investigated
for their antiproliferative activities on human cervical adenocarcinoma (HeLa) cell line at the
concentrations of 5100 g/mL by using BrdU ELISA assay during 24 h of incubation. GA exhibited
the highest activity against HeLa cells (IC50 < 5 g/mL). The potency of inhibitions (at 100 g/mL)
against HeLa cells were found as GA > A-7-G > 4-HBA. Our results showed that gallic acid exhibited
highest radical scavenging and antiproliferative effect.

KEYWORDS
Gallic acid, HeLA, BrdU ELISA, polyphenol, DPPH

Corresponding Author: FEYZA OKE-ALTUNTAS, feyzaoke@gazi.edu.tr


1
GAZ NVERSTES FEN FAKLTES BYOLOJ BLM
2
ANKIRI KARATEKN NVERSTES FEN FAKLTES KMYA BLM

1809
Poster Session 14
Submission ID: 1834

ANTIOXIDANT AND ANTICHOLINESTERASE ACTIVITIES OF THE


ETHANOL EXTRACTS FROM VARIOUS PARTS OF IN VIVO AND IN
VITRO GROWN PISTACIA LENTISCUS L.

1 2 2 2
ENGIN TLKAT , HILAL SARUHAN FDAN , ABDULSELAM ERTA , MUSTAFA ABDULLAH YILMAZ , GURBET
2 3 4 2
BALAMI , VEYSEL SZERER , FATIH MEHMET KILIN , AHMET ONAY

ABSTRACT
In the present study, anticholinesterase and antioxidant activities of the ethanol extracts from
different parts of in vivo and in vitro raised Pistacia lentiscus were studied. The DPPH free radical
scavenging activity and cupric reducing antioxidant capacity (CUPRAC) methods was used to
determine antioxidant activity. A spectrophotometric method developed by Ellman, Courtney, Andres
and Featherstone was used to determine the acetyl- and butyryl-cholinesterase inhibitory activities. In
terms of DPPH free radical scavenging activity, 6 samples prepared from root, stem and leaf parts of
the female and male genotypes and 3 in vitro samples were found to have a very high antioxidant
potential. It has been determined that samples prepared from stem parts of both female and male
genotypes have higher activities than other parts. In vivo samples were more active than in vitro
samples in both male and female genotypes. The CUPRAC activities of in vivo samples were higher
from the standarts BHT and -TOC. Absorption values in 100 g/ml concentration for female leaf,
stem,root,male leaf, stem, root, BHT and -TOC were determined as 3.06, 3.81, 3.31, 2.65, 3.80, 3.46,
3.18 and 1.69 respectiveley. There is a decrease in the activity of in vitro samples in the CUPRAC
activity. Also, in vivo samples of female and male genotypes were found to have a high
anticholinesterase enzyme activity. Among the ethanol extracts, the extracts from the stem part of
male genotype have the highest activity against both acetylcholinesterase and butyrylcholinesterase
enzymes. This study indicated that the ethanol extracts of the stem parts of both lentisk genotypes may
be a new potential resource of natural antioxidant and anticholinesterase compounds.

KEYWORDS
: Pistacia lentiscus, Antioxidant activities, anticholinesterase activities, in vitro.

Corresponding Author: FATH MEHMET KILIN, fmehmet206@gmail.com


1
BATMAN NVERSTES
2
DCLE NVERSTES
3
BNGL NVERSTES
4
KAHRAMANMARA ST MAM NVERSTES

1810
Poster Session 14
Submission ID: 1836

EXAMINATION OF ANTIMICROBIAL ACTIVITY OF PULICARIA


DYSENTERICA (L.) BERNH

1 2 2 3 1
ALI EN , MAYRAM TYSZ , SEHER BIRTEKSZ TAN , AHMET DOAN , LEYLA BT

ABSTRACT
The genus Pulicaria in Turkey is represented by 6 species, P. arabica, P. armena, P.
dysenterica, P. odora, P. sicula and P. Vulgaris. Pulicaria dysenterica is known as ayr otu, yara otu,
papatya in Turkey. In traditional medicine, it is used for constipation, the treatment of abdominal
pain, stye in eye, cold, bronchitis, inflamed wound, also as carminative, diuretic. Also the plant is used
against dysentery. In this study, the antimicrobial activities of ethanol (PDE) extract and its fractions
(hexane (PDH), chloroform (PDC), ethyl acetate (PDEA), and aqueous ethanol (PDSE)) of Pulicaria
dysenterica were also evaluated. Antimicrobial activity against Staphylococcus aureus ATCC 29213,
Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 25922, Enterococcus faecalis
ATCC 29212, Klebsiella pneumoniae ATCC 4352, Pseudomonas aeruginosa ATCC 27853, Proteus
mirabilis ATCC 14153, Candida albicans ATCC 10231, Candida tropicalis ATCC 750 and Candida
parapsilosis ATCC 22019 were determined by the microbroth dilutions technique using the Clinical
Laboratory Standards Institute (CLSI) recommendations. PDE and PDH extracts showed moderate
activity against Candida tropicalis with MIC values of 312 g/mL. All extracts (except PDE) with
MIC values of 625 g/mL had moderate activity against Candida albicans. PDH, PDC, and PDEA
extracts exhibited moderate antifungal activity against Candida parapsilosis with MIC values of 625
g/ mL. All extracts against Staphylococcus aureus were found to have poor antibacterial activity with
MIC values of 1250 g/mL. PDC showed moderate activity with a MIC value of 625 g/mL, while
PDH with a MIC value of 1250 g/mL showed weak antibacterial activity against Staphylococcus
epidermidis. This is the first study to examine the antifungal effect of Pulicaria dysenterica on Candida
species and the results of this study showed that extracts are effective on Candida species.

KEYWORDS
Examination of antimicrobial activity of Pulicaria dysenterica (L.) BERNH.

Corresponding Author: LEYLA BT, leylabitis@marmara.edu.tr


1
MARMARA UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY
2
ISTANBUL UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL MCROBOLOGY
3
3MARMARA UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL BOTANY

1811
Poster Session 14
Submission ID: 1837

DETERMINATION OF ANTIFUNGAL AKTIVITES OF PHELLINUS


IGNIARIUS AND PHELLINUS LUNDELLII

1 1 1
DICLE ERDDU , HEMEN GL ERASLAN , PERIHAN GLER

ABSTRACT
: Fusarium species, known as plant pathogens, cause bottom rot, leaf and spike burn and rot in
various cereal products. Grain such as wheat, corn oast, rye and other plant foods such as peanuts,
tomatoes and potatoes and they can form toxins. In this study, antifungal activites of two Phellinus (
Phellinus igniarius and Phellinus lundellii) species collected from Krkkale province were
investigated. Samples were taken from solid media and ethanol and methanol ekstrakt were prepared
and applied againts two Fusarium(Fusarium crookwellense and Fusarium oxysporum) by disk
diffusion method. As a result of this study, it was determined that the ekstrakts of Phellinus igniarius
prepared in ethanol and methanol had antifungal effect on Fusarium oxysporum and Fusarium
crookwellense. It has been ebserved that the ethanol-ekstrakted ekstrakt of Phellinus lundellii can not
have antifungal activity. It was observed that the ethanol and methanol ekstrakt of Phellinus lundellii
could not have antifungal activity Fusarium crookwellense.

KEYWORDS
Antifungal activites, Phellinus, Fusarium, Trkiye

Corresponding Author: PERHAN GLER, perihanguler71@gmail.com


1
KIRIKKALE NVERSTES

1812
Poster Session 14
Submission ID: 1838

THE USE OF AROMATIC PLANTS AND SPICES IN VEGETABLE


OILS: FLAVORED OILS

1 1 1 2
ENES BERBER , DERYA ARSLAN , AYENUR ACAR , HASAN HSEYIN KARA

ABSTRACT
Spices have been used by many cultures since ancient times in order to enhance the flavor and
aroma of foods, and generally have antioxidant and antimicrobial properties. Many aromatic plants
and spices are known to increase the nutritional value and shelf life of foods. Today, synthetic
antioxidants such as BHT and BHA are used as additives to increase the shelf life of oil-rich foods.
Careless use of many synthetic additives has significant health risks. Many antioxidant active
ingredients found in spices and herbs have been used to aromatize vegetable oils to increase the
oxidative stability of the product. In addition to spicy aromas, it is preferred because of the reasons
such as very low health risks, higher consumer acceptance and increased bioactive qualities of the
product. This increases the interest in spices as antioxidant sources. There are examples in the world
where various vegetable oils such as soy and canola are aromatized with aromatic herbs and spices, the
most common application in the world and in Turkey is the aromatization of olive oils. Turkish food
codex olive oil and pomace notification defines flavored olive oil as; "Olive oil which is obtained by
adding different spices, plants, fruits and vegetables and in terms of other characteristics, carries the
properties of the products of its category within the scope of this notification." Flavored olive oil is
also expressed in terms of aromatized, aromatic, gourmet and boutique olive oil in sector jargon.
Seasoning of olive oil is a process usually applied to natural extra virgin olive oils to increase
nutritional value, enrich sensory properties and prolong shelf life. When the studies carried out in this
matter are examined, aromatic plants and spices themselves can be added in crushing or kneading
stages of olive oil production as well as plant infusions, extracts or essential oils can also be used. In
addition, the incorporation rate of infusion or extracts of these different spices or plants and the time
needed for infusion/maceration have also been the subject of studies. According to many studies
carried out on olive oil, the seasoning of olive oil causes an increase in oxidative stability, along with
studies in which the reduction in antioxidant activity and amount of phenolic components are also
detected. It is reported that the combined use of aromatic plants has stronger positive effects.

KEYWORDS
Aromatized vegetable oils, aromatic plants, spices

Corresponding Author: DERYA ARSLAN, dears@konya.edu.tr


1
NECMETTN ERBAKAN NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM
2
NECMETTN ERBAKAN NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1813
Poster Session 14
Submission ID: 1840

CULTIVATION OF TRUFFLES AND DEVELOPMENT PLANS ECO-


SYSTEM MANAGEMENT FOR THE TRUFFLE INDUSTRY

1 1
AMAL EZZANEGA , LAHSEN KHABAR

ABSTRACT
Throughout, human history. Truffles have been consumed in many countries and have been
used as folk medicine and in cosmetic products. The quality and variety of truffles demanded by
peoples has increased as consumers desire more and ecologically is a key of living sustainably in
semi-arid and arid areas. In both ecological and socio-economic importance setting, the inoculating
plants with mycorrhizal fungi considered greatly a good solution for in a degraded ecosystem where
the truffles yield lost or threatened for many reasons: ecological factors influence, human activities
and methods use for harvested it. The aims of this research has demonstrated that the production of
mycorrhized plant by inoculation seedling of likely hosts plant with slurry of truffle spores is one
inoculation technique encourage the cultivation of truffles .

KEYWORDS
key words: slurry of truffle spore, mycorrhized hosts plant, cultivation.

Corresponding Author: AMAL EZZANEGA, witania2000@hotmail.com


1
UNVERSTY MOHAMMED V FACULTY OF SCENCE, DEPARTMENT OF BOLOGY, TEAM FOREST
MYCOLOGY AND TRUFFCULTURE B.P. 1014, RABAT-AGDAL , MOROCCO.

1814
Poster Session 14
Submission ID: 1842

ANTIOXIDANT AND VOLATILE COMPOSITION OF ESSENTIAL OIL


FROM MYRTUS COMMUNIS IN TURKEY

1
HLYA DEMIR

D
ABSTRACT
The isolated essential oils from seven air-dried plant species were analyzed by gas
chromatographymass spectrometry (GC-MS). The essential oil from Myrtus communis was

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characterized by the presence of -terpinene (34.4%),cineole (9.6%), linalool (6.2%),-
pinene,camphene,cineole,terpineol, terpinen-4-ol,thymol,caryophyllene. Antioxidant activity was
evaluated as a free radical scavenging capacity (RSC), together with the effect on lipid peroxidation
(LP). RSC was assessed by measuring the scavenging activity of essential oil on 2,2-diphenyl-1-
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picrylhydrazil (DPPH) and hydroxyl radical, together with the effect on lipid peroxidation (LP). RSC
was assessed by measuring the scavenging activity of essential oil on 2,2-diphenyl-1-picrylhydrazil
(DPPH) and hydroxyl radicals. Effect on LP was evaluated following the activities of essential oil in
Fe2+/ascorbate and Fe2+/H2O2 systems of induction. Investigated essential oil reduced the DPPH
radical formation (IC50 = 7.050.26 g/mL).
ES

KEYWORDS
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GC-MS analysis; essential oils; Myrtus communis, antioxidants.


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Corresponding Author: HLYA DEMR, hdemir40@gmail.com


1
YEDTEPE NVERSTES

1815
Poster Session 14
Submission ID: 1845

THE EXTREMELY USE OF OILS OBTAINED BY COLD PRESS


(COLD PRESS) OF SOME PLANTS

1 1 1
EYMA AKALIN BENDERLI , MEVLT BYKHELVACIGIL , BERIL KOPARAL

ABSTRACT
MULBERRY SEED OIL Latin: Oleum Morus alba Mulberry (Morus alba) is a tree-shaped
plant from the family Moraceae. It is known that the oil obtained from the cold press method of
mulberry cores is rich in especially essential fatty acids and vitamin E vitamins. It is stated that when
used as a mouthwash in the treatment of oral wounds due to its antiviral and antibacterial properties, it
can also be used in the treatment of throat pain, tonsillitis, improvement of mouth and teeth wounds
and in the treatment of infections caused by candida species fungus known as thrush in children. It has
also been reported that antiinflammatory, antipyretic and antitussive corticosteroids may be used to
support the treatment of diseases such as mouth-throat infections, asthma and arthritis (joint
inflammation). CHIA SEED OIL Latin: Oleum Salvia hispanica Chia (Salvia hispanica); It is a plant
of Lamiaceae family like island tea. The most important feature of oil obtained by the cold press
method of Chia seed, which is known to have many health benefits; The amount of herbal omega-3
ALA (Alpha Linolenic Acid) is at a high level. It is used for the treatment of Metabolic Syndrome
patients with the help of treatment, support for weight loss, support to increase the endurance of
athletes, anticoagulant and antihypertensive properties, cardioprotective and antiinflammatory effect,
nutritional support (salat and yoghurt ease of use by joining) are reported to be able to be used as
support in many diseases. The level of ORAC (Oxygen Radical Absorbance Capacity) indicated as an
antioxidant capacity value is very high and it is reported that it can help the athletes to increase their
stamina during competition and training. HEMP SEED OIL Latin: Oleum cannabis Cannabis oil
produced by the Cold Press method contains essential polyunsaturated fatty acids and tocopherols
such as omega 3 and omega 9. The ideal omega-3 / omega-6 ratio, containing hemp oil, is an ideal
nutritional supplement for a healthy and balanced diet. Anticonvulsant and antiepileptic properties,
antidepressant and antihypertensive properties, antioxidant and antiinflammatory properties,
hypercholesterolemia, nutritional support (ease of use by adding to salatas and yoghurts) and support
for many diseases for anti-amyloid and antiepileptic properties, help to reduce tremor in dystonic
movement disorders, healthy and balanced nutrition can be used as. FLAX SEED OIL Latin: Oleum
Lini Almost every part of flax (Linum usitatissimum) plant has commercial use, especially flaxseed is
very important in terms of food and nutrient sectors. Especially strong and durable fibers found in the
structure are of great importance in terms of usage areas. The oil obtained by cold press method from
flax seeds is predominant due to the presence of -linolenic acid (ALA, 18: 3n-3) and lignan in the
content of flax seed oil. A number of studies have shown that atherosclerosis plays an important role
in increasing secondary mortality and morbidity to cardiovascular disease (CVD). Recent studies have
shown that the presence of omega-3 fatty acids in daily diets is effective on preserving the heart, that
omega-3 fatty acid ingestion of 0.5-2 grams per day is effective in protecting against cardiovascular
diseases and that there is no additional effect of taking them at higher doses. Support for osteoporosis

Corresponding Author: EYMA AKALIN BENDERL, sakalin@zade.com.tr


1
HELVACIZADE A..

1816
and menopause treatment has been considered as a source of herbal Omega 3 (ALA), especially in
vegetarians, in adults who can not consume fish. In patients with chronic constipation (constipation) it
is known that in addition to being a good source of nutrients due to treatment support and fibrous
structures in their contents, they also increase bowel movements in adults.

KEYWORDS
Cold press, mulberry, chia, hemp, flax

1817
Poster Session 14
Submission ID: 1846

CHIA SEED: A NEW FUNCTIONAL FOOD

1 1
EHRIBAN UUZ , SEVAL ANDI

ABSTRACT

D
Chia (Salvia hispanica L.), a biannually cultivated herb, is one of the oldest crops cultivated
for centuries by the Aztec tribes (natives before Hispanic colonization) in Mexico. The seeds were
used for the preparation of medicines, foods and paintings by Aztecs and Mayas. Chia disappeared as

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crop for centuries and was rediscovered in the middle of the 20th century. The chemical composition
consists of protein (1525%), fats (25% to 40% oil with 60% of it comprising -3

KEYWORDS EN
Chia, Salvia hispanica L., functional food,
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Corresponding Author: EHRBAN UUZ, sehribanuguz@yyu.edu.tr


1
YZNC YIL NVERSTES

1818
Poster Session 14
Submission ID: 1847

ANTIBACTERIAL EFFECT OF ESSENTIAL OIL OF ROSA


DAMASCENA MILL. ON PSEUDOMONAS TOLAASII

1 1
ESIN BASIM , HSEYIN BASIM

ABSTRACT
Rose essential oil has been used to natural beauty treatments for thousands of years. Rose oil
is a good bactericide as well as it has a strong antimicrobial, antiseptic, antiparasitic and antibacterial
activity. Essential oil of Rosa damascena was obtained by steam distillation from petals of R.
damascena grown in the Isparta province of Turkey. In this study, antibacterial activities of different
vestigated by in vitro
agar diffusion and volatility tests against Pseudomonas tolaasii, a causal agent of bacterial brown
blotch disease on cultivated mushrooms (Agaricus bisporus). It was determined that the essential oil
showed an effective antibacterial activity against P . tolaasii strains comparing with those of Thymbra

potential to be used a natural protectant for bacterial brown blotch disease pathogen, Pseudomonas
tolaasii. Future detailed works needs to be completed for a practical use of the essential oil to control
bacterial brown blotch disease on cultivated mushrooms. This is a first report of antibacterial activities
of the rose oil against P. tolaasii.

KEYWORDS
Agaricus bisporus, Pseudomonas tolaasii, Brown blotch disease, Rose Oil, Rosa damascena

Corresponding Author: ESN BASIM, ebasim@yahoo.com


1
AKDENZ NVERSTES

1819
Poster Session 14
Submission ID: 1848

PRELIMINARY SEARCH FOR CAPILLARY PERMEABILITY


EFFECTS OF SELECTED MEDICINAL PLANTS AJUGA
CHAMAEPITYS, PHLOMIS GRANDIFLORA AND BONGARDIA
CHRYSOGONUM

1 1 1
CEYLAN AKA , UFUK KOCA ALIKAN , ESRA AKKOL

ABSTRACT
Objective / Purpose: Capillary permeability is meaning of permitting capacity of a blood
vessel wall. If this capacity/ability deteriorates, vascular diseases (such as atherosclerosis and
peripheral artery disease, haemorrhoid, aneurysm, peripheral venous disease and varicose veins blood
clots in veins (vte), lymphedema) occur. Our aim is to determine potential antiinflammatory effect of
Ajuga chamaepitys (L.) SCHREB. (AC), Phlomis grandiflora H. S. THOMPSON var. grandiflora H.
S. THOMPSON (PG), and Bongardia chrysogonum (L.) SPACH. (BC) by applying acetic acid-
induced vascular permeability models. Material and Methods: Shade dried plant samples were
extracted with pure methanol, and the extracts were evaporated to the dryness. Previously applied
method was used for the animal experiment (Whittle, 1964; Yesilada and Kupeli, 2007). According to
that method, each plant extract (400 mg/kg dose) was administered orally to a group of 6 BALB/c
mice, waited for 30 min then the mice were injected with 0.1 mL of 4% Evans blue at the tail, after 10
min waiting period 0.5% (v/v) AcOH was injected i.p. and waited for 20 min. The mice were killed by
dislocation of the neck, and the viscera were exposed and irrigated with distilled water, which was
then poured into 10 mL volumetric flasks through glass wool, 0.1 mL of 0.1N NaOH solution was
added, each flask was made up to 10 mL with distilled water. The absorption of the final solution was
measured at 590 nm. In control animals, instead of the extract 0.5% CMC was given orally, and they
were treated in the same manner as described above. Results: According our preliminary results, %
inhibition values of acetic acid induced capillary permeability were determined as Ajuga chamaepitys,
Phlomis grandiflora and Bongardia chrysogonum) respectively. AC and PG have higher potent effect
on capillary permeability. These two plants are hopeful for vascular diseases. The results will be
discussed.

KEYWORDS
capillary permeability, acetic acid-induced, phlomis, ajuga, bongardia

Corresponding Author: CEYLAN AKA, akaceylan@gmail.com


1
GAZ NVERSTES ECZACILIK FAKLTES

1820
Poster Session 14
Submission ID: 1850

INVESTIGATION OF MACRO AND MICRO MINERAL CONTENT OF


SOME MEDICINAL AND AROMATIC HERBS AND IT'S INFUSION

1 2 1
ADEM GNE , ERMAN BEYZ , BAAK ORU

D
ABSTRACT
Some of the elements to create organic structures are found in trace amounts, but in some

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cases they are too large to be toxic or toxic. While the levels of the trace play an important role in the
fulfillment of metabolic activites, their deficiencies or toxic amounts are also causing diseases. In this
study, crop raw and packaged sage, cumin, nigella and melissa were collected which grow naturally in
our country. These herbs direct and hot water extraction are analysed to check variations of the
element contents (K, Na, Ca, Mg, P, Cu , Fe, Zn, Ni, Mn, Cr, Pb). As a result of the studies done on
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the plants, the nutrient contents of the plants differed. This study was determined cumin is the highest
element content and melissa is the lowest element content in herbs samples. As a result of the
examinations, Pb content of toxic element is higher in Cemen plant, Cd content is higher in Cemetery
plant. In general, the amount and diversity of macro-micro elements and heavy metals found in
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medical plants can vary depending on the physical, chemical and biological properties of the soil and
climate factors.

KEYWORDS
PR

Medicinal herbs, macro and micro mineral


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Corresponding Author: ADEM GNE, ademgunes@erciyes.edu.tr


1
ERCYES NVERSTES SEYRAN ZRAAT FAKLTES TOPRAK BLM VE BTK BESLEME
2
ERCYES NVERSTES SEYRAN ZRAAT TARLA BTKLER

1821
Poster Session 14
Submission ID: 1851

EFFECIENCY OF PROPOLIS EXTRACT-DELIVERY


NANOCOMPOSITE SYSTEM ON WOUND CARE

1 1
HATICE KBRA ARIKAN , HAYRIYE HALE SOLAK

D
ABSTRACT
Propolis is a strongly adhesive resinous substance collected, transformed and used by bees to

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seal holes in their honey combs, smoot houtthe internalwalls and protect the entrance against intruders
It has a long history of being used in traditional medicine and been reported to have a broad spectrum
of biological activities, such as anticancer, antioxidant, antiinflammatory, antibiotic,
andantifungalactivities The biological or pharmacological activity is associated with phenolic
compounds, mainly with potential antioxidantnamely flavonoids andaromaticacids. Flavonoids are
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frequently used as the main index for product evaluation of propolis. Propolis has recently become
popular as a health drink and it claimed to prevent diseases such as dermatological wounds,
inflammation, heart disease, diabetes, cancer, etc. Electrospinning is a unique fiber spinning technique
of producing nanofibers from a large variety of bulk starting materials in a moderately easy, repeatable
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and simple fashion.It is a simple and inexpensive process that uses an electric field to control the
formation and deposition of nanoormicro-sized polymeric nanofibers from a liquid polymer.
Electrospun nanofibrous fabrics with high specific surface area, aspectratio and porosity as a result of
random deposition of fibers, couldhave a greatpotential in biomedical applicationssuch as tissue
engineering scaffolds, drug delivery carriers, wound dressings etc. In this study, potential of propolis
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extract (bee glue) solution based electrospun nanocomposite fabrics as wound dressing were
investigated macroscopically in vitro experiments. PVA polymer with different concentrations were
dissolved in water at 60 oC and then propolis exract was added into polymer solution. %5 PVA / %5
Propolis sol., %7 PVA / %7 Propolis sol., %9 PVA / %9 Propolis sol., nanocomposite fabrics were
produced by electrospinning method over polypropilen nonwoven fabrics. SEM analyses tests were
applied for the nanocomposite fabrics as wound dressing. In-vitro experiments showed that
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antimicrobial effectiveness of bacterial solutions with nanocamposite fabrics were better than those of
not including nanocomposite fabrics bacterial solutions. According to investigations nanocomposite
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fabrics with propolis sol. were provided antimicrobial effect against to gram positive bacteria (S.
aureus) and not provided antimicrobial effect against to gram negative bacteria (A baumannii and P.
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aeruginosa). The results indicated that the electrospun PVA/Propolis extract nanocomposite fabrics as
wound dressing provided a good means for wound healing caused by gram positive bacteria.

KEYWORDS
Nanocomposite, PVA polymer, propolis, wound dressing

Corresponding Author: HATCE KBRA ARIKAN, haticekubraarikan@yahoo.com


1
KAHRAMANMARA ST MAM NVERSTES

1822
Poster Session 14
Submission ID: 1852

THE DETERMINATION OF WILD MEDICAL PLANTS GROWING IN


ERZURUM AND USE OF THEM AGAINST VARIOUS DISEASES

1 1 2 3 4
AKAR KARAKO , SERAP BUZTEPE , EYYUP TELC , AYHAN BUZTEPE , ELMAS KARAKO

D
ABSTRACT
As in whole world, in our country, plants existing in the natural flora for different purposes

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has been used as a part of traditional cultere from past to today. Plants are of great importance in the
prevention and treatment of diseases or the maintenance of health. According to the investigations of
World Health Organization, there are approximately 20.000 plants used for medical purposes. Our
country also has a rich flora in terms of medicinal plants. Medical plants are widely used by the people
in the city of Erzurum located in eastern Anatolia. The aim of present study is to determine which wild
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medicinal plants growing in Erzurum are used for which illness by the people living in this city. This
study was carried out on 73 volunteers, were selected randomly, living in Erzurum. The mean age of
they was 41.34 15.23 (mean standard deviation). 34 (46.6%) of the participants were women, 39
(53.4%) were men. The data were collected through a questionnaire, also including open-ended
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questions, prepared by the researchers. it was asked which wild medicinal plants growing in erzurum
had been used for which illness by participants. Also, participants' preferences were asked about plants
and medicines in order to use in the treatment of diseases. Descriptive statistics, chi-square and
Fisher's exact tests were performed for the statistical analysis of data. In this research, 31 different
medicinal plants were detected, growing in Erzurum and used against various diseases. The findings of
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the research showed that the most commonly consumed plants are giant fennel (Ferula communis)
(39.7%), Rheum ribes (known as kn" in East Anatolian of Turkey) (19.2%), Nettle herb (Urtica
dioica L.) (13.7%), rosehip (Rosa canina L.) (11%), mallow (Malva sylvestris) (9.6%), rumex crispus
L. (8.2%). 68.9% of the participants consumed giant fennel herb stated that this plant was used for
diabetes mellitus, and 31.1% were used for the treatment of infectious and respiratory diseases, and
cancer and hypertension. It was found that the Rheum ribes was used for cancer (42.8%), diabetes
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mellitus (28.6%), respiratory and gastrointeninal diseases. Nettle herb was used for cancer (40%),
respiratory (20%), infectious (20%) and rheumatic (10%) diseases, and diabetes mellitus (10%). All
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participants using rosehip consumed it against respiratory diseases. The participants stated that they
used mallow in the treatment of the infectious, respiratory and gastrointeninal diseases. In addition to,
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it was determined that rumex crispus L. was commonly used in hemorrhoids treatment (50%).
Participants' preferences (plants, medicines or both of them) were asked for the treatment of diseases.
The preferences was compared with social security, economic situation, sex, education levels,
occupation and age groups (the youngs, adults and the olds). All of the participants who did not have
social security preferred to use the plant. No significant difference found between the age groups and
the preference, but most of the olds (50%) preferred plants to medicines. There was no significant

Corresponding Author: AKAR KARAKO, akarkarakoc@gmail.com


1
VOCATONAL SCHOOL OF HEALTH SERVCES, ATATURK UNVERSTY
2
MNSTRY OF HEALTH
3
MNSTRY OF FOOD, AGRCULTURE AND LVESTOCK
4
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, MUGLA STK KOCMAN UNVERSTY, MUGLA,

1823
difference between women and men n terms of preferences, but it was found that most of men
(53.8%) preferred using the plants. The results of this research showed that most commonly consumed
wild medicinal plants by people living in Erzurum are giant fennel, rheum ribes, Nettle herb, rosehip,
mallow, rumex crispus L.. The wild medicinal plants are used, especially, against diabetes, cancer,
hemorrhoids, and infectious, respiratory, gastrointestinal and rheumatic diseases. Biological activity
studies are needed to understand the effects of these plants on the treatment of these diseases.

KEYWORDS
Health, Wild Medicinal plants, Erzurum

1824
Poster Session 14
Submission ID: 1853

INVESTIGATION OF SOME LAVENDER ESSENTIAL OILS


COMPONENTS USING UV, FT-IR AND GC ANALYSIS

1 2 2 2
SEDA KLEN , FATMA EROLU , SEBLA DNER , MERVE GMSOY

ABSTRACT
ABSTRACT In recent years, the value of medical and aromatic plants has increased
considerably. The flowers of Lavandulastoechas, which grow in maki areas of Western Anatolia, are
used as analgesic and expectorants. English lavender (L.angustifolia) is used as essence. Lavender
essential oil samples were analyzed using gas chromatography to determine the levels of camphor,
linalool and linalyl acetate which is the criteria of the essential oil quality. The purpose of this study
was to determine some spectroscopic and chromatographic properties of essential oils isolated from
lavender (L. angustifolia), Lavandula stoechas and lavandin (Lavandula x intermedia) harvested in
2016 in Middle Anatolia. The essential oils, isolated by steam distillation, were analyzed by gas
chromatography (GC), FT-IR and UV. In the FT-IR spectra OH, C=C, C=O and C-O bending and
streching peaks were determined. In the UV analyzes, characteristic absorption bands of linalool,
linalylacetate and flavanoids, which are important main components of lavander, were observed in the
range of 200-270 nm.

KEYWORDS
Lavander, essential oil, UV, FTIR

Corresponding Author: FATMA EROLU, feroglu@ankara.edu.tr


1
TARLA BTKLER MERKEZ ARATIRMA ENSTTS MDRL, KALTE DEERLENDRME VE GIDA
BLM, ANKARA
2
ANKARA NVERSTES, FEN FAKLTES, KMYA BLM, ANKARA

1825
Poster Session 14
Submission ID: 1854

SPECTROSCOPIC INVESTIGATION OF ESSENTIAL OIL


COMPONENTS IN SOME THYME SPECIES

1 2 1 1
FATMA EROLU , SEDA KLEN , SEBLA DNER , MERVE GMSOY

ABSTRACT
In many industries such as medicine, food, perfume and cosmetics, essential oils obtained
from plants are used as raw materials. Thyme, is an important aromatic and medicinal plant containing
an essential oil used widely in medicine and food industry. By the method of water steam distillation
of thyme, 2 - 8%, an aromatic oil is obtained. These volatile fats are carvacrol and thymol from
monoterpene phenols. Thyme essential oil has important properties such as antispasmodic,
antirheumatic, antiseptic, bactericidal, diuretic, expectorant, insecticide etc. The purpose of this study
was to determine some spectroscopic and chromatographic properties of essential oils isolated
fromThymusvulgaris, Saturejaspicigera and Origanumvulgaresubsp.Gracile, harvested in Middle
Anatolia. These essential oils, isolated by water steam distillation, were analyzed by
gaschromatography (GC), UV and FT-IR. In the UV analyzes, characteristic absorption bands of
carvacrol and thymol, which are important main components of thyme, were observed in the range of
200-275nm. In the FT-IR spectra bending and stretching peaks of phenols are observed.

KEYWORDS
Thyme, essential oil,UV, FTIR

Corresponding Author: FATMA EROLU, feroglu@ankara.edu.tr


1
ANKARA NVERSTES, FEN FAKLTES, KMYA BLM, ANKARA
2
TARLA BTKLER MERKEZ ARATIRMA ENSTTS MDRL, KALTE DEERLENDRME VE GIDA
BLM, ANKARA

1826
Poster Session 14
Submission ID: 1855

ALTERNATIVE FOR THE FUTURE: NATURAL FOOD COLORS

1 2 1 1 3
KRAN AKIR ARICA , SELDA Z , AYE ZYILMAZ , SEVIL DEMRC , ABDULLAH KSZ

ABSTRACT
Color additives are used to color many beverages, foods, and sweets in the world for many
reasons. For example, food colors are used by the food and beverages industry to improve the color of
the food which is lost while their processing. Many synthesized dyes were easier and less costly to
produce and were superior in coloring properties when compared to naturally derived alternatives.
These compounds have drawn considerable attention in recent years, not because of their coloring
properties, but due to their potential healthpromoting effects. Amounts of artificial food dyes and
added sugars in foods and sweets commonly consumed by children. Clinical guidance is given to help
caregivers avoid artificial food dyes and reduce the amount of sugars in childrens diets. Based upon
many cell-line studies, animal models, and human clinical trials, it has been suggested that many
natural food colorants, due to their potent antioxidant property, possess anti-inflammatory and anti-
carcinogenic activity, cardiovascular disease prevention, obesity control, and diabetes alleviation
properties. The food dyes market is segmented based on types which include synthetic and natural
colors. Synthetic colors still hold a major share, but natural colorants are the fastest growing segment
due to consumer consciousness especially in developed countries. The aim of this study is to draw
attention to the value of natural food colors in terms of human health and to highlight Turkey's
potential for natural food colorants.

KEYWORDS
Color additives, natural food colorants, natural antioxidants

Corresponding Author: KRAN AKIR ARICA, sukran.cakir@iste.edu.tr


1
SKENDERUN TEKNK NVERSTES
2
KIRIKKALE NVERSTES
3
NECMETTN ERBAKAN NVERSTES

1827
Poster Session 14
Submission ID: 1857

SORPTION OF ANTHOCYANINS FROM HIBISCUS FLOWERS ONTO


DIATOMITE FROM AQUOEUS SOLUTION

1 1
ZKAN DEMRBA , ERDAL TALA

ABSTRACT
Anthocyanins are natural colorants found in various colors such as orange, pink, red, violet to
blue in the flowers and fruits of the vascular plants in nature. Anthocyanins are harmless and water
soluble. Another important feature of anthocyanins is antioxidant activity, which is known to play a
vital role in preventing neuronal and cardiovascular diseases, cancer and diabetes. The main colorant
of the hibiscus flower is cyanidin-3-sophoroside. Diatomite is a silica sedimentary rock formed in the
form of an amorphous silica containing a small amount of microcrystalline material in its structure.
Considering these properties, in this study, the sorption kinetics or rates of the main colorants of
hibiscus flowers to the diatomite surface in aqueous solutions was investigated. The determination of
sorption, adsorption or desorption kinetics or rates is very important for elucidating the mechanism of
these phenomenon. For this purpose, a series of experiments were carried out in order to better explain
the mechanism of the sorption of the cyanidin-3-sophoroside molecule to the diatomite surface. The
constants of the pseudo first and second order kinetics equations are calculated with the experimental
data. Mass and intra-particle diffusion constants are also determined. Dried hibiscus flowers and
diatomite particles used in the experiments were grounded and sieved to have a particle size below 50
micrometres. UV-Vis spectra of hibiscus flowers in different masses in aqueous solutions were as
follows. The maximum wavelength, max, from the graph is 520 nm for aqueous solutions containing
hibiscus flowers. A linear relationship was observed between the hibiscus mass in the solution and the
absorbance values of the solution at 520 nm. Thus, quantitative analysis has also been done in this
study. Experimental studies were carried out with various parameters. These are solution temperature,
time, equilibrium pH of solution and the mass and particle size of the hibiscus flowers and diatomite in
solution. As a result of the experimental studies, no effect of the contact time on the sorption was
determined. However, increasing pH and solution temperature increase sorption rate, while particle
size decreases. In addition, the particles were characterized by FTIR-ATR, SEM and BET surface area
measurements and TG analyzes.

KEYWORDS
Sorption, hibiscus flowers, diatomite, anthocyanins, kinetics

Corresponding Author: ZKAN DEMRBA, ozkandemirbas@gmail.com


1
BALIKESR NVERSTES

1828
Poster Session 14
Submission ID: 1858

QUALITATIVE AND QUANTITATIVE ANALYSIS OF NATURAL


ANTIOXIDANT SAGE(SALVIA OFFICINALIS)

1 1 1
AYE AKAN , FATMA EBRU KO , ALAR KULU

ABSTRACT
Sage (Salvia Officinalis) is a 50-100 cm high, purplish blue flowering, simple leafy, perennial
and bitter plant. It grows on mountain slopes, is widely cultured. 1-2.5% contains essential oil, tannins
and bitter substances. Theyre very rich in phenols, flavonoids and flavonols. The so-called "apple oil"
or "bitter apple oil" is also produced from Anatolian sage. Sage is widely used for cold due to
antiinflammatory, antimicrobial effect1,2 In this study, the sage plant collected from the
Mediterranean region was extracted with different solvents and methods, and the yields were
compared. The quantities of rosmarinic acid, carnosic acid and carnosol, which are responsible for the
antioxidant capacity of the sage plant, were analyzed qualitatively and quantitatively by an HPLC
device; The volatile oils in the extract were determined by GC-MS instrument and the total phenol
content in terms of gallic acid in UV-VIS spectrophotometer was also examined. For this purpose, 70
mesh sieves were sieved after the sage plant was treated. Maceration were done for sages at 45 C
with 70% ethyl alcohol in varying ratios (1: 6) and time (3; 6; 8; 10 hours); 100% methanol and 100%
ethanol extractions; Soxhlet extraction efficiencies were compared. The proportions of rosmarinic acid
and the amounts of carnosic acid and carnosol were analyzed in the extracted at UV detector and 280
nm wavelength by a Thermo Scientific Ulimate HPLC instrument. As a result of the experiments, a
maceration method with 70% ethanol with 25% extraction yield was chosen. As a result of
calculations on the standard graphic of rosmarinic acid in analytical standards,; It was determined that
7.45 mg "rosmarinic acid"; 3,42 mg carnosol + carnosic acid in gram 70% ethanol extract of sage;
Volatile components of the ethanol extract were detected on the Agilent brand GC-MS; The extract
was found to contain high levels of cineol, camphor, borneol components.

KEYWORDS
Sage(Salvia Officinalis), Extract, HPLC.

Corresponding Author: AYE AKAN, ayse.akan@aksuvital.com.tr


1
AKSUVTAL AR-GE MERKEZ

1829
Poster Session 14
Submission ID: 1859

KINETICS OF INTERACTION BETWEEN SILICA SURFACES AND


ANTHOCYANINS FROM HIBISCUS FLOWERS

1 1
ZKAN DEMRBA , ASLIHAN RMEYSA TEMZ

ABSTRACT
Anthocyanins occur in all tissues of higher plants, including leaves, stems, roots, flowers, and
fruits. Anthocyanins are glycosides of anthocyanidins, the basic chemical structure was given in
Figure 1a. Cyanidin-3-sophoroside, the main colorant of the hibiscus flower, is also an anthocyanin.
Despite the great potential of applications that anthocyanins represent for food, pharmaceutical, and
cosmetic industries, their use has been limited because of their relative instability and low extraction
percentages. Their use in textile is negligible as they lack affinity for the fiber and cannot sustain
washing. Currently, most investigators are engaged in solving the problems that are associated with
isolation and stability of anthocyanins, their purification, identification and their end uses. For this
purpose, in this study, the sorption kinetics of the cyanidin-3-sophoroside molecule, main colorants of
hibiscus flowers, to the silica surface in aqueous solutions was investigated. Dried hibiscus flowers
and silica particles used in the experiments were grounded and sieved to have a particle size below 50
micrometres. UV-Vis spectra of hibiscus flowers in different temperature in aqueous solutions were
given in Fig. 1b. The maximum wavelength, max, from the graph is 520 nm for aqueous solutions
containing hibiscus flowers. Experimental studies were carried out with various parameters. These are
solution temperature, time, equilibrium pH of solution and the mass and particle size of the hibiscus
flowers and silica in solution. As a result of the experimental studies, no effect of the contact time on
the sorption was determined. However, increasing pH and solution temperature increase sorption rate,
while particle size decreases. The constants of the pseudo first and second order kinetics equations are
also calculated with the experimental data. Mass and intra-particle diffusion constants are also
determined. In addition, the particles were characterized by FTIR-ATR, SEM and BET surface area
measurements and TG analyses.

KEYWORDS
Sorption, hibiscus flowers, silica, anthocyanins, kinetics

Corresponding Author: ZKAN DEMRBA, ozkandemirbas@gmail.com


1
BALIKESR NVERSTES

1830
Poster Session 14
Submission ID: 1861

RATIONAL USE OF MEDICAL AND AROMATIC PLANTS

1
ADUMAN KARACA

ABSTRACT

D
Throughout the history of mankind, plants are known not only for food but also for long-
lasting medical use as the data gathered from many archaeological excavations suggest. "Today, more
than 375,000 plants have been identified in the world, but it is registered in the literature that only

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about 20,000 of them have been utilized by the people. A large proportion of the vast majority of
plants make up the medical and aromatic plant group "(Ceylan, 1996). Today, medical and aromatic
plants (TAB) are generally grouped as spice, cosmetics, paint, pleasure, aromatherapy and
phytotherapeutic use. Within the framework of this presentation, TAB will only cover medical plants,
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therefore plants that are included in field of therapy (phytotherapy). The fact that most of the world's
diseases can not be treated and even diseases becoming more complicated from day to day for the last
20-30 years, has been engaging health institutions and individuals not only in our country but all over
the world. In parallel with advancing technology, the emphasis on human health increases day by day,
and the desperation against illnesses is also worrying. While alternatives are being sought for the
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treatment of diseases all over the world, the past treatment methods have come to the fore as a remedy
for today's health problems. Phytotherapy stands for Plant Therapy, so it is useful to emphasize the
rationality of plant drugs prepared for therapeutic purposes, because if the plant drug is not
salutiferous, the disease process will further progress and even new diseases will be created
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involuntarily rather than healing it. Thus, since the Hippocrates, the physician has acted on the
rationale of "Do not harm human beings first", which is the main rule. The second important step in
the rational use of medical plants is the competence of the person who is practicing this profession.
Here, it is essential to have a basic medical knowledge consisting primarily of the anatomy,
physiology and pathology of the human organism. With this knowledge, a physician does not yet have
a qualification to treat with plants. It also requires the healing knowledge of plants, which requires a
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thorough education. Only by integrating the knowledge of both of them, the physician will initiate a
rational, healthy and long-term treatment process by taking the subject as an individual and applying a
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holistic treatment of the diseases as much as possible. The planned presentation is intended to present
both the ideal criteria for plant drugs to the treatment practitioners in a rational way, as well as the
rules that the specialists who will be practicing with plant drugs should pay attention to in the ideal
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environment, especially through the case of Germany, the country where this profession is applied in
the most advanced stance through legal regulations and practices.

KEYWORDS
PHYTOTHERAPY, RATIONAL USE OF MEDICAL AND AROMATIC PLANTS

Corresponding Author: ADUMAN KARACA, karacasaduman@hotmail.com


1
SKDAR NIVERSTES

1831
Poster Session 14
Submission ID: 1862

THE MPORTANCE OF SAFFLOWER (CARTHAMUS TINCTORIOUS


L.) AS A MEDICINAL PLANT, REGARDING ITS ACTIVE
SUBSTANCES

1 1 1
FATIH SEYIS , EMINE YURTERI , AYSEL ZCAN

ABSTRACT
More than 200 components are isolated from different parts (leave, flower and seed) up to
now. The most known components are: flavanoids, phenylethanoids, cumarins, fatty acids, steroids
and polisaccarides.7 seretonin derivatives were isolated from safflower oil. The main components of
safflower flowers is carthamin, an important flavanoid glycosides. ts flowers contains also
arthamidin, isocarthamidin, quercetin, kaempferol,6-hydroxykaempferol and its glikosides and
hydroxysafflor yellow A, safflor yellow A, safflamin C, safflamin A, safflomin-A. Also lauric,
miristic, palmitic, linoleic, arachidic and flavon luteolin and luteolin 7-O-beta-D-glucopyranoside and
luteolin-7-O-(6''-O-acetyl)-beta-D-glucopyranoside are presents in Carthamus tinctorius flowers.
Nikotiflorin, are present as an natural flavanoid in in the petals of C. tinctorius. An seconder
metabolite, alkan-1,3-diols, was isolated from dried petals of safflower. The main essential oil
componenrs of safflower flowers were determined as Caryophyllene, p-allyltoluene,1-acetoxytetralin
and heneicosane. An new quinokalkon C-glikoside, tinctormine was determined in safflower. The
potentail use of safflower in phytotherapy and developments in its use as healing agent was discussed
in detail. t was determined that safflower has antioksidant, analgezic, antidiabetic, hepatoprotektic ve
antihperlipidemic activities.

KEYWORDS
safflower, medicinal plant, active substances

Corresponding Author: EMNE YURTER, emine.yurteri@erdogan.edu.tr


1
RECEP TAYYP ERDOAN NVERSTES

1832
Poster Session 14
Submission ID: 1863

CHEMICAL CONTENT AND IMPORTANCE FOR


COMPLEMENTARY MEDICINE OF TEA (CAMELLIA SINENSIS L.)

1 1 1 1
AYSEL ZCAN , EMINE YURTERI , YUSUF AVATLI AVATLI , FATIH SEYIS

ABSTRACT
Tea belongs to the genus Camellia, family Theaceae, division Angiospermae in the plant
kingdom. There are types with bushy forms as well as the single-stem types in cultivated types of tea
plant. This plant is everygreen and the most valuable and most consumed drink after the water in all
over the world. Tea produced from cultivated forms of the species Camellia sinensis (L) O. Kuntze
can be obtained from the terminal bud and fresh leaves under this terminal bud forming at the tip of
the young shoots on the cultivated plant. The black tea most consumed between processed tea products
is obtained by withering, curling, oxidation, drying, classification and packaging processes of these
fresh and non-fibber leaves. Four tea types can be obtained from plant: green tea, white tea, oolong tea
and black tea. These tea yypes have medicinal and therapeutic effects in terms of their chemical
compositions. The widely produced and traded tea types contain protein, carbohydrates, oils,
polysaccharides, vitamins and minerals, caffeine, quercetin, kaempferol, flavonoids (catechin,
epicatechin, epicatechin gallate, epigallocatechin, epigallocatechin gallate), gallic acid, chlorogenic
acid, tanen and and amino acid (total of 19 including theanine) in their structures. It has protective
effects against coronary heart diseases, stroke, cardiovascular diseases, cancer, obesity, hypertension,
arthritis, viral and inflammatory diseases and effects regulating to bone density due to its high
antioxidant properties and chemical content. In this review, the bioactive substances in various tea
types obtained from tea plants which is medically important and the importance of complementary
medicine of these substances are discussed in detail.

KEYWORDS
Tea, complementary medicine, chemical composition

Corresponding Author: AYSEL ZCAN, aysel.ozcan@erdogan.edu.tr


1
RECEP TAYYP ERDOAN NVERSTES

1833
Poster Session 14
Submission ID: 1865

THE MULTIPLICATION OF ORIGANUM SPP. COLLECTED FROM


RIZE HIGHLANDS WITH SEED AND PERLITE

1 1 2 1
FATIH SEYIS , EMINE YURTERI , KUDRET KEVSEROLU , AYSEL ZCAN

ABSTRACT
The aim of this study was to determine the multiplication potential using seeds and cuttings of
Origanum spp. collected from the highlands of Rize. Cuttings were obtained from material collected
from nature and cuttings were cutted from the 2nd and 3rd node and were implemented with 1000
ppm and 1500 ppm IBA for 1 min and transferred to peat containing perlite. Seed were directly
transferred to peat containg soil and the results showed us that success rate was 25 % in seed
multiplication and 80% in multiplication with cuttings at 1000 ppm and 40 % in multiplication with
cuttings at 1500 ppm..

KEYWORDS
Origanum, multiplication, perlite

Corresponding Author: FATH SEYS, fatih.seyis@erdogan.edu.tr


1
RECEP TAYYP ERDOAN NVERSTES
2
ONDOKUZ MAYIS NVERSTES

1834
Poster Session 14
Submission ID: 1866

MEDICINAL MPORTANCE OF WHITE TEA (CAMELLIA SINENSIS


L.)

1 1 1 1
EMINE YURTERI , FATIH SEYIS , AYSEL ZCAN , YUSUF AVATLI

D
ABSTRACT
It is possible to say that tea is the most consumed drink on the World after water. Specially f

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Turkish tea is mentioned it has to be considered that tea takes it place at breakfast as well as in 5 o
clock service and also after meal as an irreplceable part of our culture. Tea is nowadays grouped
acccording to its fermentation level as green and white (not fermented), partially poisoning
(fermented) and oolong tea in three groups. The surplus or rarity of phemolic compounds in tea shows
variation depending besides genotype/environment on cultivation package and on the process
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technology up to the last stage where tea is packaged. With the increase in number of processes in tea
processing an increase in a and thearubigins and an decrease in the catechin group is given. The
importance of unprocessed white tea is rising because of its high catechin content compared with other
teas. Poliphenols, specially catechin derivatives are strong antioxidant agents anda have explicit
characteristics like oxidative stress inhibition and clenaing free radicals. t is well known that
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oxidatiev damages are the reasons of diseases arising with develepment of industry in the last years
and it is also known that these damages are causing cardiovascular diseases, diabetes, neural crest
damage and cancer in humans. Therefore, these antioxidants present in white tea are preventing the
initiation or development o these diseases where endogenous defences are insufficient against reactive
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species.

KEYWORDS
White tea, health, medicine
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Corresponding Author: FATH SEYS, fatih.seyis@erdogan.edu.tr


1
RECEP TAYYP ERDOAN NVERSTES

1835
Poster Session 14
Submission ID: 1870

RESEARCH SOME OF QUALITY CHARACTERISTICS AND DRUG


YIELD OF CULTURED MNT(MENTHA PIPERITA L.)

1 1
AYE SOLUK , YUKSEL KAN

ABSTRACT
This research has been conducted under Konya ecological conditions to determine the effect
on yeld and quality some characters of nitrogen fertilizers applied at the different doses of Mentha
piperita in Medicinal Aromatic Plants laboratory and Medicinal and Aromatic plants Experimental
Farm of Agriculture Faculty, Selcuk Universty. Experiment was designed and applied in randomized
complete plot design with three replications in the year of 2012-2013. According to results of this
researh; plant height 31.50-48.50 cm, plants, drug herba yield 307,8-949,0 kg / da, essential oil yield
1.7-2.3 % and major essential oil component (mentol) was varried between 28.06 and 34.29 %. for M.
piperita . According to the results of this research; The highest drug yield and essential oil yield for
mint in Konya and similar ecology 10 kg/da nitrogen fertizer application is reasonabled.

KEYWORDS
M. piperita, fertilizer, essential oil, menthol

Corresponding Author: AYE SOLUK, aysesoluk0@gmail.com


1
SELUK NVERSTES

1836
Poster Session 14
Submission ID: 1871

DETERMINATION OF OPPORTUNITY TO USE AND HABIT OF


MEDICINAL AND AROMATIC PLANTS IN ORDU PROVINCE
(TURKEY)

1 1 1 1
MERYEM YEIL , EMEL KARACA NER , MEHMET MUHARREM ZCAN , AYEGL KIRLI

ABSTRACT
In this research, it is aimed to show the opportunity of usage of medical plants and
consumption habits which the concentration of most of the consumption in which medicinal plants of
the people living in the province of Ordu. In this regard, such a comprehensive study has not been
found before in the provinces and districts of Ordu. In the study, the questions to be asked in the
questionnaire were carefully selected and the questionnaire survey was completed with face to face
interviews with 310 people in the Ordu center and districts.It was applied to people aged 18 years and
over by convenience sampling. The obtained data were analyzed with descriptive statistical methods
and the habit of using medical plants by the people living in Ordu provinces, the most used medical
plants, the use of medical plants in the treatment of diseases and answering questions such as if they
are used, which diseases are used most frequently?.According to the results of the research, medicinal
plant use which has been determined to be the most used medical and aromatic plant in the province of
Ordu as mint, thyme, sage, lime and garlic and has emerged medicinal plant use has been complained
as diseases such as colds, digestive system disorders, sedatives, sinusitis / migraine, blood pressure
regulator. The vast majority of respondents indicated that they use medicinal plants for the treatment
of diseases, and also the second place is to treat both illnesses and maintain health. Also for the
question where do you get the medical plants from? mostly the choice is pick up myself from the
herbalist and nature .

KEYWORDS
Medicinal plants, usage habits, species, diseases, questionnaire

Corresponding Author: MEHMET MUHARREM ZCAN, mehmetmuharrem@gmail.com


1
ORDU NVERSTES

1837
Poster Session 14
Submission ID: 1880

RESEARCH SOME OF QUALITY CHARACTERISTICS AND DRUG


YIELD OF CULTURED MNT(MENTHA SPICATA L.)

1 2
AYSE SOLUK , YUKSEL KAN

ABSTRACT
This research has been conducted under Konya ecological conditions to determine the effect
on yeld and quality some characters of nitrogen fertilizers applied at the different doses of Mentha
spicata in Medicinal Aromatic Plants laboratory and Medicinal and Aromatic plants Experimental
Farm of Agriculture Faculty, Selcuk Universty. Experiment was designed and applied in randomized
complete plot design with three replications in the year of 2012-2013. According to results of this
researh; plant height 43.88-64.36 cm, drug herba yield 479,6-1283.00 kg / da, essential oil yield 1.4-
2.0 % and major essential oil component (carvon) was varried between 49.70 and 61.50 % for M.
spicata. According to the results of this research; The highest drug yield and essential oil yield for mint
in Konya and similar ecology 10 kg/da nitrogen fertizer application is reasonabled.

KEYWORDS
M. spicata, fertilizer, essential oil, carvone

Corresponding Author: AYSE SOLUK, aysesoluk0@gmail.com


1
SELUK NVERSTES

1838
Poster Session 14
Submission ID: 1890

ANTIFUNGAL EFFECTS OF THE ESSENTIAL OILS OF CUMIN,


LAUREL AND THYME AGAINST MYCOGENE PERNICIOSA, A
CAUSAL AGENT OF WET BUBBLE DISEASE ON CULTIVATED
MUSHROOM AGARICUS BISPORUS (LANGE) SING.

1 1
ESIN BASIM , HSEYIN BASIM

ABSTRACT
The essential oils of cumin (Cuminum cyminum L.), laurel (Laurus nobilis L.) and thyme
(Thymbra spicata L.), which are consumed as spices were tested in vitro for their antifungal effects
against Mycogene perniciosa, a causal agent of wet bubble disease of the cultivated mushroom
(Agaricus bisporus (Lange) Sing.). Antifungal effects of the different doses

investigated in vitro for inhibition effect on mycelial growth of the M. pernisiosa. The sterile
deionized water was used as a control. The 5 mm diameter disc taken with cork-borer from colony
side of the 7-days pathogen fungus was placed on Potato Dextrose Agar (PDA) medium. The different
doses of the essential oils were placed the middle of the petri dish cover.The petri dishes were
wrapped by parafilm as well as the petri dishes were reversed in position and incubated at 270C. The
even lower doses of Thyme oil showed 100% antifungal effect on M. perniciosa mycelial growth. The
antifungal effects of the cumin and laurel on M. perniciosa were different according to the doses of the
essential oils used. The cumin, laurel and thyme,essential oils have potential to be used as natural
agents for controlling wet bubble disease pathogen, M. pernisiosa. A practical use of the essential oils
needs to be completed future detailed works on direct cultivated mushrooms. This is a first report of
antifungal activities of the cumin, laurel and thyme oils against M. pernisiosa.

KEYWORDS
: Agaricus bisporus, Mycogene perniciosa, Cumin Oil, Laurel Oil, Thyme Oil, Wet Bubble
Disease

Corresponding Author: ESN BASIM, ebasim@yahoo.com


1
AKDENZ NVERSTES

1839
Poster Session 14
Submission ID: 1891

ASSESMENT OF -AMYLASE, -GLUCOSIDASE,


CHOLINESTERASE INHIBITION AND ANTIOXIDANT ACTIVITY OF
ALCEA BIENNIS, ARUM DIOSCORIDIS AND GERANIUM LUCIDUM

1 1 1 1
HASYA NAZLI EKN , DDEM DELORMAN ORHAN , LKAY ERDOAN ORHAN , MUSTAFA ASLAN

ABSTRACT
Diabetes mellitus is a growing health problem worldwide causing severe and costly
complications including blindness, cardiac and kidney diseases and increases the risk of cognitive
impairment and dementia. The high glucose level of diabetic patients is the most important factor for
the increased formation of free radicals such as superoxide which causes oxidative damage. It is
considered that insulin signalling dysregulation is important contributing factors in Alzheimers
disease pathogenesis. The aim of the study is to determine antidiabetic, antioxidant and anti-alzheimer
effect of Alcea biennis, Arum dioscoridis and Geranium lucidum -amylase and -glucosidase enzyme
inhibition methods were used to examine antidiabetic activity of 80% ethanol extracts of the plants.
Acetylcholinesterase (AChE) and Butyrylcholinesterase (BChE) inhibition methods were used to
determine antialzheimer activity. DPPH and DMPD radical scavenging, metal-chelation, ferric-
reducing antioxidant power (FRAP) assays were utilized for screening of antioxidant activity. Total
flavonoid and phenolic content of the extracts were calculated. Among the tested extracts G. lucidum
extract (95.050.77%) displayed the strongest -glucosidase inhibitory activity. All of the extracts
were inactive against AChE. All plant extracts displayed lower inhibition than 50% inhibition against
AChE and BChE. G. lucidum extract (1.40420.072) showed high FRAP activity. Our findings
indicated that G. lucidum contains potential compounds having selective -glucosidase inhibitory
activity and our work is in progress to identify their active components.

KEYWORDS
Alzheimer, Antioxidant, Diabetes, Alcea biennis, Arum dioscoridis, Geranium lucidum

Corresponding Author: HASYA NAZLI EKN, hasyaekin@gmail.com


1
GAZ NVERSTES

1840
Poster Session 14
Submission ID: 1892

HEPATOPROTECTIVE EFFECT AND PHENOLIC PROFILE OF THE


POLLEN ETHANOL EXTRACT OF PINUS BRUTIA TEN.

1 2 3 4 1
HASYA NAZLI EKN , HNA GUL , MUHAMMAD GULFRAZ , NLGN ZTRK , LKAY ERDOAN ORHAN

ABSTRACT
The genus Pinus L. belongs to the family Pinaceae. Pinus brutia Ten. has the widest
distribution area in Turkey. The pollens from P. brutia are used internally to enhance body resistance
in recent years. In this study, hepatoprotective activity of the ethanolic pollen extract (80 %) of P.
Brutia, which is known to use againt hepatic disorders in some parts of Turkey was administrated by
gavage to CCl4-induced Swiss albino mice at various doses (100, 200, and 300 mg/kg). The effects on
triglyceride, HDL, LDL, and cholesterol levels as well as on liver marker enzymes, e.g.
alanineaminotransferase (ALT), aspartateaminotransferase (AST), alkaline phosphatase (ALP), and
total bilirubin, RBC, WBC as well as platelet level of mice were investaged. The extract caused a
signicant decrease on triglyceride, HDL and LDL cholesterol, liver enzymes, and protein level
compared with the CCl4 group (p<0.05). Blood cells were improved with the extract level compared
with the CCl4 group (p<0.05). These ndings were further approved by histopathological observations
of liver cells of mice and it was expected that restoration of these parameters might be due to presence
of some bioactive compounds. Therefore, the phenolic profile of the pollen ethanol extract was
determined. In conclusion, traditional use of P. brutia pollens against hepatic disorders has been
confirmed in vivo.

KEYWORDS
Pinus brutia, Hepatoprotective

Corresponding Author: HASYA NAZLI EKN, hasyaekin@gmail.com


1
GAZ UNIVERSITY
2
QUAD-AZAM UNIVERSITY
3
COMSATS
4
ANADOLU UNIVERSITY

1841
Poster Session 14
Submission ID: 1900

USES OF MEDICAL AND AROMATIC PLANTS IN LANDSCAPE


RESTORATION WORKS

1 2
HSEYIN AHIN , MURAT ERTEKIN

D
ABSTRACT
In the 21. century, rapidly developing technology and industrialization, as being a parallel

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with this development, the raw metariel need is increasing constantly. So, made a big production to
meet this raw metarial is caused increasing of mining areas. The mining operation that can be made
large production is the open pit operation. In this study, the effects of open pit operation to natural area
is examined and explained how to apply of landscape repair for activity of finished areas. In this
context, operated in order to make a production of sand + clay and after that in the landscape
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restoration of the sample area is explored by Sile Forest Management Directorate in the area of Saritas
Ridge in Yesilvadi Village which is connect to Sile, Istanbul. The most important point of chosen this
area, to be a sample application to the landscape restoration works in the future for our country in
order to landscape restoration to the field, as planting the medicinal plants, aromatic plants and as well
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as stone pine. The datas of the measured characters of planted types are evaluated in the SPSS
Statistical Program.

KEYWORDS
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Mining, planting , medical and aromatic plants, geographic information system, landscape
restoration
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Corresponding Author: MURAT ERTEKN, mertekin@konya.edu.tr


1
2
NECMETTN ERBAKAN NVERSTES GZEL SANATLAR FAKLTES

1842
Poster Session 14
Submission ID: 1901

NEW TECHNIQUES IN PROPAGATION AND BREEDING OF BAY


LAUREL (LAURUS NOBILIS L.)

1
MURAT ERTEKIN

D
ABSTRACT
An investigation was carried out to consider the effect of polystimulins (PS), gibberellic acid

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(GA3) and stratification date on the growth of laurel seedlings. A total of 1200 seed treated with PS-
A6+ PS-K and GA3 at two different concentrations before stratified, and a total of 315 seedling were
investigated taking account of height, collar diameter, root length and number of leaves. To conclude,
it is obvious that hormone application is very effective and has contributed significantly to the
metabolism of laurel seedlings. According to this study, the application of GA3 to laurel seedlings has
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been recommended for practical use in nursery situations.

KEYWORDS
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Laurus nobilis, Seed, Seedling, Germination, Pre-sowing Treatment
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Corresponding Author: MURAT ERTEKN, mertekin@konya.edu.tr


1
NECMETTN ERBAKAN NVERSTES GZEL SANATLAR FAKLTES

1843
Poster Session 14
Submission ID: 1902

EFFECTIVE METHODS ON DORMANCY BREAKING OF SEEDS OF


AILANTHUS ALTISSIMA (MILL.) SWINGLE

1 2
MER LTF ORBACI , MURAT ERTEKIN

D
ABSTRACT
This study was conducted in an attempt to break dormancy and thus enhance germination of

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tree of heaven (Ailanthus altissima) seeds. The another aim is to bring new proposals to uses for
environmental design. Prior to sowing, seeds were treated as follows: (i) cold stratification of seeds at
4C for 30 and 60 days; (ii) soaking in 500 mg/l polystimulin (PS-A6+ PS-K), gibberellic acid (GA3),
or benzylaminopurine (BAP) for 24 h and stratification for 30 days; or (iii) soaking in 100 ml/l
effective microorganisms (EM 1), 5000 mg/l PS-A6+ PS-K, GA3 and BAP for 72 h, followed by
stratification for 45 days. EN
KEYWORDS
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Tree of heaven, dormancy, seed, pre-sowing treatment, ornamental plant
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Corresponding Author: MER LTF ORBACI, omerlutfucorbaci@hotmail.com


1
RECEP TAYYP ERDOAN NVERSTES GZEL SANATLAR, TASARIM VE MMARLIK FAKLTES
2
NECMETTN ERBAKAN NVERSTES GZEL SANATLAR FAKLTES

1844
Poster Session 14
Submission ID: 1903

ALLERGENIC PLANTS OF ANKARA, TURKEY

1 2 3
CENNET TEKIN CRE , MER LTF ORBACI , MURAT ERTEKIN

ABSTRACT

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In this study, allergen-bearing species in parks and green areas in Ankara city center are
mentioned. While plant types mentioned; it was evaluated separately as broad-leaved trees, handed
trees and trees, broad-leaved trees, climbers and climbers, perennials and seasonal flowers. The

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evaluations provided detailed information about the general characteristics of the plant, flowering
times, fruit formation, allergenicity, ecological requirements, and whether the species is domestic or
foreign.

KEYWORDS
EN
Allergy, Allergenic plant, Pollen, Community health, Landscape
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N

Corresponding Author: MURAT ERTEKN, mertekin@konya.edu.tr


1
ANKARA NVERSTES, ZRAAT FAKLTES, PEYZAJ MMARLII BLM
2
RECEP TAYYP ERDOAN NVERSTES GZEL SANATLAR, TASARIM VE MMARLIK FAKLTES
3
NECMETTN ERBAKAN NVERSTES GZEL SANATLAR FAKLTES

1845
Poster Session 14
Submission ID: 1910

EFFECTS OF ADDITION OF TEREBINTH, SESAME AND FLAXSEED


FLOUR TO BISCUIT FORMULATION ON THE QUALITY AND
SHELF LIFE OF THE BISCUIT

1 1
AYENUR USLU , NILGN ERTA

ABSTRACT
Shelf life is an important quality parameter in biscuits. One of the criteria that determines the
shelf life is lipid oxidation. During the oxidation of the lipids in foods, the bad taste and smell that
occurs as a result of the reaction. In recent years some researches asserted that oxidation products
could be serious threat to health. Some of these compounds even have a carcinogenic effect. For this
reason, the addition of natural antioxidant compounds to prevent lipid oxidation is mentioned by the
researchers. Terebinth, flaxseed, sesame seeds are rich in antioxidants. Terebinth contain important
minerals and vitamins, such as B and E vitamins, sodium, phosphorus, potassium, magnesium, iron,
calcium, zinc, manganese, copper, cadmium and selenium. Sesame seeds have a strong antioxidants
such as sesamol, sesamin, sterols and E vitamin. The oils obtained from of sesame seeds, show a
considerable antioxidant effect according to the amount of tocopherols present in the sesame seeds.
Flaxseed is an important herbal source of health beneficial compounds. The lignan in flaxseed
contains has antioxidant properties. The flaxseed, rich in -linolenic acid and good quality protein, is
also a natural source of phytochemicals such as flavonoid, lignan and phenolic acids. Flaxseed is
generally classified as "functional food", "bioactive food" and / or "endocrine active food". In order to
increase the shelf life and improve the quality of the biscuits, terebinth, sesame and flaxseed flour
added to biscuit formulations. In this study, the physical properties (Diameter thickness, spread ratio),
chemical properties (moisture, ash, protein), nutritional properties (phytic acid, total amount of
phenolic substance, mineral substance), and shelf life properties (PH, determination of induction
times, determination of peroxide) were determined in biscuits enriched with terebinth, sesame and
flaxseed flour.

KEYWORDS
Biscuit, terebinth, sesame seeds, flaxseed, antioxidant

Corresponding Author: NLGN ERTA, dr.nilgunertas@gmail.com


1
NECMETTN ERBAKAN NVERSTES

1846
Poster Session 14
Submission ID: 1911

BUCKWHEAT (FAGOPYRUM ESCULENTUM MOENCH) IN HEALTH


IMPORTANCE AND FEATURES AS A FUNCTIONAL FOOD

1 2
EMINE FAYDAOLU , MEHMET REFIK YCEL

ABSTRACT
In this study, the relation of buckwheat to health is evaluated and its features as a functional
food will be emphasized. The buckwheat plant has a significant positive effect on health with its
naturally occurring components. It is also an important food product for celiac patients because it does
not include gluten. Being a functional food, it can be used as raw material in many food items or it can
only be consumed as cooked. The phenolic materials, antioxidants and fiber components increase the
functional properties and nutritional value of the food which the buckwheat is added. In bread
technology and in the production of baked goods, some problems such as low bread volume and
hardness are caused. Allergen proteins have been studied which limit the use of buckwheat by
enzymatic route or by fermentation.

KEYWORDS
Buckwheat, gluten-free goods, health, functional foods.

Corresponding Author: EMNE FAYDAOLU, eminefaydaoglu@gmail.com


1
ORMAN VE SU LER BAKANLII, SU YNETM GENEL MDRL
2
TE DANIMANLIK NGR AR-GE ETM TCARET THALAT HRACAT

1847
Poster Session 14
Submission ID: 1912

DETERMINATION OF ANTIOXIDANT CONTENT OF TURKISH PINE


(PINUS BRUTIA TEN.) PEEL EXTRACT

1 1 2 3
PELIN EROLU , GKTRK AVAR , ALI OSMAN ADIGZEL , RUKEN ESRA DEMIRDEN , FATIH MEHMET
4 5 1 1
EMEN , TUNCAY YEILKAYNAK , DERYA YKSEL , BARI SALI

ABSTRACT
Turkey has the highest Turkish pine tree population. Turkish pine, which is such widely
spread, is used in forestry industry. Generally plant phenolics constitute natural antioxidant resources.
Pine peel extract is rich in polyphenols. Antioxidants effectively fight against free radicals and prevent
the damage to the cells. Moreover, Turkish pine peel extract contains a polyphenol called oligomeric
proanthocyanidin (OPC) besides these compounds, which are very precious for human health. Data
indicate that OPC has very important effects on our health. It is believed that processing Turkish pine
peels would provide great health benefits especially for preventing cancer. Besides this Turkish pine
peels, which offers a great potential as raw material for us, would also make it possible to provide
added value to the pharmaceutical and cosmetic sector. In this study, determination of antioxidant and
antibacterial content of the extract, which would be obtained from Turkish pine peels, was targeted.
Turkish pine peels are ground. The powder thus obtained was extracted for 6 hours in a soxhelet
extractor. It was determined that the ethanol extract of Turkish pine showed more than 50% inhibition
in a concentration range between 1-10 mg/mL. However, it was determined that the radical scavenging
effect of Turkish pine extract decreased as the concentration increases. Nevertheless, it was observed
that this decrease was at a low ratio. When incubation periods are considered an inhibition above 50%
was observed in 30 minutes at all concentrations. It was determined that according to this the
concentration at which the radical scavenging effect would be the maximum is 1mg/mL.

KEYWORDS
Antioxidants, proanthocyanidin, pinus brutia ten, Turkish pine tree

Corresponding Author: GKTRK AVAR, gokturkavsar@yahoo.com


1
MERSN UNVERSTY, SCENCE AND LETTERS FACULTY, CHEMSTRY DEPARTMENT
2
MERSN UNVERSTY, SCENCE AND LETTERS FACULTY, BOLOGY DEPARTMENT
3
ANKIRI KARATEKN UNVERSTY, FACULTY OF SCENCE, CHEMSTRY DEPARTMENT
4
MEHMET AKF ERSOY UNVERSTY, SCENCE AND LETTERS FACULTY, CHEMSTRY DEPARTMENT
5
KAHRAMANMARA ST MAM UNVERSTY, AFN VOCATONAL SCHOOL

1848
Poster Session 14
Submission ID: 1915

HUMAN HEALTH EFFECTS OF PSEUDO-CEREAL

1 1
ELIF NCEL , MUSTAFA KRAT DEMR

ABSTRACT
HUMAN HEALTH EFFECTS OF PSEUDO-CEREAL Elif NCEL, M. Krat DEMR
Cereals and cereal products constitute a large part of our daily diet. However, cereal-like products are
at the forefront, as common grains contain gluten protein and people with celiac disease should not
consume gluten-containing foods. Pseudo-cereals in this context (quinoa, buckwheat and amaranth)
are a good alternative due to gluten-free and high nutritional values. In addition to being a good source
of protein, pseudo-cereals with balanced amino acid content also possess antioxidant properties.
Quinoa, buckwheat and amaranth are a good source of vitamin-E and are thought to potentially have
cholesterol-lowering properties with these characteristic. In recent years clinical studies have shown
that amaranth as well as being an alternative to celiac disease, have also anticarcinogenic, antiallergic,
antihypertensive and cholesterol-lowering properties due to anti-tumoral peptides such as lunasin and
some other components. Buckwheat also has important phenolic components. Rutine in its content,
which can be seen as a good cardiovascular drug for the treatment of disorder, are diglucosides
flavanols. Rutine also reduces high blood pressure and prevents hemorrhagic diseases. On the other
hand, polyphenols contained in quinoa have a great importance due to antioxidative and potential
anticarcinogenic effect, which suggests the possibility of strengthening the immune system against
cancer. In this review; the phenolic and antioxidative properties of quinoa, buckwheat and amaranth
which have both balanced amino acid content and significant phenolic components have been
mentioned.

KEYWORDS
Pseudo-cereal, Amaranth, Buckwheat, Quinoa

Corresponding Author: ELF NCEL, e.oncell@hotmail.com


1
NECMETTN ERBAKAN NVERSTES FEN BLMLER ENSTTS GIDA MHENDSL ANA BLM DALI

1849
Poster Session 14
Submission ID: 1921

PHYTOCHEMISTRY AND BIOACTIVITY OF NATURAL PRODUCTS


FROM CEPHALARIA TUTELIANA

1 1 1
MERVE DALI , SUHEYLA KIRMIZIGL , NAZLI SARIKAHYA

ABSTRACT
Natural products are the source of numerous therapeutic agents. Recent progress to discover
drugs from natural sources has resulted in compounds that are being developed to treat cancer,
resistant bacteria, viruses and disorders [1]. Cephalaria genus which is belonging to Caprifoliaceae
family has 94 species in Turkey. These species are widely spread out in Mediterranean Region [2].
Triterpenoid metabolites are the major class of this genus as well as iridoid, flavonoid, lignan
glycosides and alkaloids. Cephalaria species have also many biological activities such as antioxidant,
antimicrobial, antifungal and cytotoxic activities [3-7]. Considering this knowledge, our purpose is to
isolate the seconder metabolic components from Cephalaria tuteliana which is an endemic plant
collected from Istanbul-Turkey. The isolation and purification studies on n-BuOH fraction of C.
tuteliana were performed by chromatographic (CC, TLC, VLC, MPLC) and chemical methods.
Structures of the isolated natural compounds were determined with the help of different spectroscopic
techniques (IR, 1D-, 2D-NMR, MS, HRMS, GC-MS). The potential biological activities of these
natural compounds were also investigated. References 1. Ikan, R. Naturally occurring glycosides,
Chichester, England: John Wiley & Sons, (1999). 2. Kus, S., Gokturk, R.S., 2005. Nordic J. Bot.,
23:27-430. 3. Sarikahya, N.B., Kirmizigul, S., 2010. J. Nat. Prod., 73:825-830. 4. Tabatadze, N., Elias,
R., Faure, R., DePauw-Gillet, M., Kemertelidze, E., Chea, A., Ollivier, E., 2007. Chem. Pharm.Bullet.,
55:102-105. 5. Kirmizigul, S., Boke, N., Sumbul, H., Gokturk, R.S., Arda, N., 2007. Pure App. Chem.,
79:2297-2304. 6. Zviadadze, L.D., Dekanosidze, G.E., Dzhikiya, O.D., Kemertelidze, E.P., 1983.
Khim. Prir. Soedin., 1:46. 7. Yazicioglu, T., 1978. J. Am. Oil Chem. Soc., 55:412.

KEYWORDS
Cephalaria tuteliana, triterpene sapoin, iridoid, bioactivity

Corresponding Author: NAZL SARIKAHYA, nazli.sarikahya@ege.edu.tr


1
EGE UNVERSTY, FACULTY OF SCENCE, CHEMSTRY DEPARTMENT

1850
Poster Session 14
Submission ID: 1922

SYNTHESIS OF GYPSOGENIN-CHALCONE HYBRID COMPOUNDS

1 1 2 2
SAFIYE EMIRDA-ZTRK , N. GKE ULUSOY , NURAN KAHRIMAN , VILDAN SERDAROLU , NURETTIN
2
YAYLI

ABSTRACT
Gypsogenin aglycone (3-Hydroxy-23-oxoolean-12-en-28-oic acid), a natural saponin, is
isolated from many plants belonging to carnations family (Caryophyllaceae). Gypsogenin with the
pentacyclic triterpenic structure is a natural compound. It is well know that gypsogenin aglycone with
sugar chains has shown a variety of biological properties such as antiviral, antitumor, anticarcinogenic,
antioxidant and anti-cancer. However the gypsogenin aglycone that obtained from water extract of
Gypsophila arrostii roots. Water used in making the halvah, is obtained from boiling water of
Gypsophila arrosti plant roots. are obtained by boiling water. On the other hand, chalcones are natural
aglycone compounds which display a wide range of biological activities, such as anticancer,
antimitotic, antiinammatory, antituberculosis, antimalarial, antileishmanial, nitric oxide regulation
modulatory, cardiovascular, and antihyperglycemic, activities. when a biodynamic heterocyclic system
was combined with another, obtained natural products are the most challenging class of compounds
for the total synthesis, due to their structural diversity and complexity as well as the interesting
biological activity. Therefore, we designed and synthesized new gypsogenin-chalcone hybrid
compounds in this study. Firstly, Gypso-Anhd compound was synthesized by substation reactions
involving acetylation at C-3 in gypsogenin aglycone. Nevertheless, methoxy substituted chalcones
were synthesized. Then, in the third step of the study, Gypso-Anhd compound was combined with
chalcone derivatives by using DCC/DMAP in DCM. Up to now, in our continuous research, we
synthesized new gypsogenin-chalcone compounds (1-3). The synthesized compounds were established
by IR, UV, 1H NMR, 13C NMR, and LCMS analyses.

KEYWORDS
Gypsogenin, Chalcone, Hibrid compounds

Corresponding Author: SAFYE EMRDA-ZTRK, safiye.ozturk@ege.edu.tr


1
EGE NVERSTES
2
KARADENZ TEKNK NVERSTES

1851
Poster Session 14
Submission ID: 1927

KEFIRAN FILMS

1 2
EDA NDL KO , MAHMUT INAL

ABSTRACT

D
Edible films are thin layers of materials used to protect and preserve many food products. The
use of these protective coatings and suitable packaging by the food industry has become a topic of
great interest because of their potentiality for increasing the shelf life of many food products. The

TE
largest part of materials used in packaging industries is produced from non-renewable material with
the negative environmental consequence. Today, there is a big effort to extend the shelf life and
enhance food quality while reducing packaging waste has encouraged the exploration of new biobased
packaging materials, such as edible and biodegradable films from renewable resources. Water-soluble
EN
polysaccharides such as starch, chitosan, cellulose derivatives, alginate, carrageenan and pectin can
form polysaccharide-based edible films. Kefir grains, the starter for obtaining the sour fermented milk
kefir, are gelatinous irregular masses, composed of proteins and polysaccharides that contain LAB,
acetic acid bacteria and yeasts involved in the fermentation. This polysaccharide component is a
heteropolysaccharide containing equal amount glucose and galactose. This work focus on a review of
ES
the available literature on the properties and production of edible films based on kefiran.

KEYWORDS
PR

kefir, kefiran, edible films, exopolysaccharide


T
O
N

Corresponding Author: EDA NDL KO, edaondul@hotmail.com


1
YZNC YIL NVERSTES
2
HAKKAR NVERSTES

1852
Poster Session 14
Submission ID: 1928

KEFIRAN EXOPOLYSACCHARIDE

1 2
EDA NDL KO , MAHMUT INAL

ABSTRACT

D
Kefir is a fermented dairy product with viscous, whitish color, and effervescent property.
Lactic acid, asetic acid, ethanol, carbon dioxide and other compounds such as acetaldehyde, diacetyl
and acetoin occur as a result of fermentation in the kefir product. Kefiran, a neutral polysaccharide that

TE
constituted by glucose and galactose produced by Lactobacillus kefiranofaciens, a lactic acid
bacterium present in kefir grains and also in fermented product such as milk and whey. Kefiran, which
has GRAS (generally recognized as safe) status, has been found to improve rheological properties of
fermented milk. This exopolysaccharide has also several health promoting properties including
EN
antimicrobial activity, immunomodulating activity, anti-inflammatory activity and antiproliferative
activity. Kefiran can be used as a food additive for its reological properties. It has viscoelastic
properties and is able to form gel at low temperature. It can use suitable packaging in food industry
because of its potentiality for increasing the shelf life of many food products. In this study, we will
focus on chemical and physical characteristics and health benefits of the exopolysaccharide produced
ES
by kefir grains during milk fermentation.

KEYWORDS
PR

Kefir, kefiran, kefir grain, exopolysaccharide, glucogalactan


T
O
N

Corresponding Author: EDA NDL KO, edaondul@hotmail.com


1
YZNC YIL NVERSTES
2
HAKKAR NVERSTES

1853
Poster Session 14
Submission ID: 1929

EVALUATION OF ANTIHEMORRHOIDAL ACTIVITY OF CAPSELLA


BURSA-PASTORIS ON EXPERIMENTAL HEMORRHOIDS IN RATS

1 1 1 1
BETL APAYDIN YILDIRIM , ABAN KORDAL , SERKAN YILDIRIM , FATIH YILDIRIM

ABSTRACT
In this study was aimed to investigate effects on the levels of plasma and rekto-anal tissue
some biochemical parameters of Capsella bursa-pastoris extract on hemorrhoid rats. A total of 48
Spraque Dawley female rats, weighing 200-250 g were used in the present study. Thirty six rats were
randomly assigned into 6 experimental groups (8 rats per group). They were allowed to adapt to
laboratory conditions for 7 days. The study was conducted for 18 days. Rats were divided into the
following groups: Group I: Control Group, Group II: Croton oil, Group III: Capsella bursa-pastoris
extract, Group IV: Croton oil + Capsella bursa-pastoris extract, Group V. Capsella bursa-pastoris tea
and Group V: Croton oil+ Capsella bursa-pastoris tea. After 3 days of croton oil application, rats were
treated with Capsella bursa-pastoris for 15 days. Blood and recto-anal tissue samples were collected
for biochemical and histopathological studies. Plasma and recto-anal tissue GSH, CAT, GPx and SOD
activity were significantly decreased in compaired to CO group. Despite this decrease, plasma TNF-,
IL-6 and MDA; recto-anal MDA and MPO levels increased in this group. In contrast plasma and
recto-anal tissue GSH, CAT, GPx and SOD activity were significantly increased, plasma TNF-, IL-6
and MDA; recto-anal MDA and MPO levels decreased in following treatment with CBE and CBT. In
conclusion, we recommend both extract and tea can be used safely for the treatment of hemorrhoids in
the large population of patients who do not want to undergo surgery and do not want to use drugs or
cream due to their many side effects but C. bursa pastoris tea gave more positive results than extract
form as biochemical and histopathological findings. C. bursa pastoris tea can improve the health and
quality of life of people who suffer from hemorrhoid disease.

KEYWORDS
Capsella bursa-pastoris, extract, hemorrhoid, oxidative stress, rat

Corresponding Author: BETL APAYDIN YILDIRIM, betul_apaydin@hotmail.com


1
ATATRK NVERSTES

1854
Poster Session 14
Submission ID: 1930

EXTRACTION OF LAVENDER (LAVANDULA OFFICINALIS),


GENTIAN (HYPERICUM PERFORATUM) AND STINGING NETTLE
(URTICA DIOICA) PLANTS GROWN IN MERSIN REGION AND
INVESTIGATION OF THE ANTIOXIDANT AND ANTIBACTERIAL
ACTIVITY OF THEIR EXTRACTS

1 2 1 3 1
GKTRK AVAR , ALI OSMAN ADIGZEL , PELIN EROLU , FATIH MEHMET EMEN , DERYA YKSEL , IDEM
1 4 5
GL , RUKEN ESRA DEMIRDEN , TUNCAY YEILKAYNAK

ABSTRACT
With its geographical place, climate and plant variety, agricultural potential and wide surface
area Turkey is one of the leading countries in plant trade. This importance of Turkey is due to the
plants, which give many of the products that constitute input to herbal medicine, plant chemicals, food
and additives, cosmetics and perfume industries of developed countries, in the flora of our country.
The main factor, which makes plants valuable, is the valuable chemical compounds they contain. The
dirty air, which we breathe all day, harmful sun-rays, dangerous substances in the bad food, additives,
unconscious eating habits and sedative lifestyle causes substances called free radicals to be produced
in the body. The molecules, which minimize and block the effect of free radicals in the body, stop the
chain reactions that may lead to many diseases and early aging, are called antioxidants. Our body
responds to these activities via antioxidants. Antioxidant molecules are the substances which protect
our body against radicals that trigger many diseases such as cancer, inflammatory, coronary and
atherosclerotic diseases. Antimicrobial substances placed in cosmetics provide protection against
microbial deterioration. Therefore, plant extracts which have antimicrobial properties commonly used
in cosmetics. In this study, the extracts of some plants (i.e., Lavandula angustifolia, Hypericum
perforatum and Urtica dioica) grown in the geography of Mersin region and which have commercial
value were obtained. Antibacterial and antioxidant properties of these extracts were investigated and
the extracts showed significant antibacterial effect. Collected plant materials were extracted with
ethanol by Soxhlet. The antimicrobial activity of Lavandula angustifolia, Hypericum perforatum and
Urtica dioica extracts against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram
negative) was assessed by the agar-well diffusion method. One loop S. aureus and E. coli colonies
were inoculated to 10 mL Nutrient Broth including 3 g/L meat extract and 5 g/L peptone and then
incubated at 37 C for 24 h. Population density of S. aureus and E. coli cultures was determined by
McFarland standard and 100 L bacterial suspension containing approximately 1.0 x 106 CFU/mL
cell was spread on to Nutrient Agar. The well was filled with 100 L plant extract (50 mg/mL).
Subsequently, the plates were incubated at 37 C for 24 h. The diameter of the inhibition zones was
measured as millimeters. The results showed that Urtica dioica, Lavandula angustifolia, Hypericum

Corresponding Author: GKTRK AVAR, gokturkavsar@yahoo.com


1
MERSN UNVERSTY, SCENCE AND LETTERS FACULTY, CHEMSTRY DEPARTMENT
2
MERSN UNVERSTY, SCENCE AND LETTERS FACULTY, BOLOGY DEPARTMENT
3
MEHMET AKF ERSOY UNVERSTY, SCENCE AND LETTERS FACULTY, CHEMSTRY DEPARTMENT
4
ANKIRI KARATEKN UNVERSTY, FACULTY OF SCENCE, CHEMSTRY DEPARTMENT
5
KAHRAMANMARA ST MAM UNVERSTY, AFN VOCATONAL SCHOOL

1855
perforatum extracts have inhibition effect on growth of E.coli and S. aureus. Additionally, it was
determined that these plant extracts were more effective on growth of S. aureus than that of E.coli. It
might be due to the differences in cell wall structure of bacteria. The results may suggest that extracts
of Lavandula angustifolia, Hypericum perforatum and Urtica dioica which have antimicrobial
properties can be used for prevention self-life of cosmetics.

KEYWORDS
Lavandula angustifolia, Hypericum perforatum and Urtica dioica

1856

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