Domingo, April Joy A.

201410779
Company: DENR-EMB CALABARZON Region

DAILY WORK ACTIVITIES

WEEK 7

Day Date Daily Accomplishment No. of Working

Hours

Measurement of Biological Oxygen

Monday May 15, 2017 Demand. Serial Dilution Bacterial 8
Analysis.

Measurement of Dissolved Oxygen.

Serial Dilution Bacterial Analysis.
Tuesday May 16, 2017 8
Preparation of the media for bacterial
analysis.

Wednesday May 17, 2017 Measurement of Dissolved Oxygen. 8

Test for ammonia and phosphate.

Friday May 19, 2017 8
Measurement of Dissolved Oxygen.

Total hours: 32
 WEEK 7
Monday, May 15, 2017

Week 7 is the busiest week of my On-the-Job training so far. Everyday there were
analysis. On Monday, six (6) samples were delivered to the laboratory. They were immediately
measured for Biological Oxygen Demand. Per usual, samples that are only measured for BOD
are directly transferred to fill up the BOD bottle, where one millilitre (1mL) of each MnSO4 and
alkali iodide were added.

After the bottles were shaken, the precipitate were allowed to settle until the it covered
only one-third () of the solution then one millilitre (1 mL) of concentrated H2SO4 was added.
After shaking the bottles, it was titrated with Na2S2O3, until pale yellow then a starch indicator
will be added and the titration continued until the solution became colorless.

Figure 1. Measurement of Biological Oxygen Demand

Serial dilution bacterial analysis were also usually done together with the measurement
of BOD whenever there were new delivered samples. The method is still the same as described
before. Where it will start with the transferring of eleven milliliters (11-mL) of the pure sample to
the bottle containing diluent solution then it was shaken and placed on its original place.

Ten milliliters (10-mL) of the pure sample was transferred to the sample bottle containing
the LTB (Lauryl Tryptose Broth; for the detection of coliforms in water). The eleven milliliters
(11-mL) of the diluted sample is transferred to another bottle containing diluent solution then it
was shaken and placed on its original place. Ten milliliters (10-mL) of the diluted sample was
transferred to the sample bottle containing the LTB. the process continues until all the bottle
containing diluent solutions were used up.

Figure 2. Serial Dilution Bacterial Analysis

Tuesday, May 16, 2017

This day, seven (7) samples were delivered to the laboratory. These samples were
measured for dissolved oxygen. After the samples were prepared and seeded, one millilitre
(1mL) of each MnSO4 and alkali iodide were added. The procedure from here are just the same
with the measurement for BOD.

Figure 3. Measurement of Dissolved Oxygen

 For the bacterial analysis, serial dilution was still the method that was used.

Figure 4. Serial Dilution Bacterial Analysis

Wednesday, May 17, 2017

For wednesday, there were fifty (50) samples where measured for dissolved oxygen.
Polyseed was prepared by dissolving it in distilled water and was stirred for an hour before
adding it to the sample bottles containing 15-20mL of the pure samples. It was then diluted
using the prepared dilution water. The procedure from here are just the same with the
measurement for BOD.

Figure 5. Measurement of Dissolved Oxygen

 Friday, May 19, 2017

Since there were some days that work cant be done, many samples were not yet tested
for ammonia and phosphates. That is why there were forty-nine (49) samples were tested for
ammonia and thirty-six (36) samples were tested for phosphates. The first thing we did is
prepare Erlenmeyer flasks (50-mL) for the samples to be tested for ammonia and labelled them
before transferring twenty-five millilitres (25-mL) of samples there. One millilitre (1-mL) of
phenol-alcohol reagent, sodium nitroprusside and an oxidizing agent. The samples were left for
an hour or so to wait for the reaction the complete before reading it in the UV-VIS
Spectrophotometer.
For the test for phosphates, Erlenmeyer flasks (250-mL) were prepared and labelled
then twenty-five millilitres (25-mL) of samples where transferred. Ascorbic acid, 4M H2SO4 and
oxidizing agent were added. It was immediately read in the UV-VIS Spectrophotometer because
unlike the test for ammonia the reaction is a lot quicker.

Figure 6. Preparation and Measurement of the Ammonia and Phosphate

Concentration in Water
 After the test for ammonia and phosphates, measurement for dissolved oxygen was
done. It wasnt a lot so it was done quite fast. There were only 30 samples to be measured.

Figure 7. Dissolved Oxygen Measurement