Universidad del Valle Guatemala Rodrigo Muralles

Bioingeniera Carnet: 12262

Tarea 1

Tabla de datos

Concentracin Concentracin glucosa

Tiempo (min) Celular (g/L) (mol/L)
0.00 10.00 10.00
0.50 10.00 10.00
1.00 10.00 10.00
2.00 10.42 9.99
3.00 10.85 9.99
4.00 11.30 9.98
5.00 11.77 9.98
7.50 13.03 9.96
10.00 14.43 9.94
15.00 17.70 9.89
20.00 21.70 9.84
25.00 26.61 9.77
30.00 32.62 9.69
40.00 49.05 9.46
50.00 73.74 9.11
60.00 110.85 8.60
70.00 166.66 7.82
80.00 250.56 6.66
90.00 376.69 4.91
100.00 566.31 2.27
105.00 607.60 1.70
110.00 636.40 1.30
115.00 658.00 1.00
120.00 665.20 0.90
130.00 665.20 0.90

Clculos
Universidad del Valle Guatemala Rodrigo Muralles
Bioingeniera Carnet: 12262

Ln(CN) dt Ln(CN)/dt rN 1/CS Cn/rN Yx/s

2.302585093 0 0.1 #DIV/0!
2.302585093 0 0.000 0.1 #DIV/0! #DIV/0!
2.302585093 0.041141943 0.000 0.1 #DIV/0! #DIV/0!
0.100100 24.80952
2.343727036 0.040438044 0.420 1 38 42
0.100100 25.23255
2.38416508 0.040637646 0.430 1 81 #DIV/0!
0.100200 25.11111
2.424802726 0.040751196 0.450 4 11 45
0.100200 25.04255
2.465553921 0.040680188 0.470 4 32 #DIV/0!
0.100401 25.85317
2.567254391 0.040821993 0.504 61 46 63
0.100603 25.76785
2.669309373 0.040851053 0.560 62 71 70
0.101112 27.06422
2.87356464 0.040749524 0.654 23 02 65.4
0.101626
3.077312261 0.040794965 0.800 02 27.125 80
0.102354 27.09775 70.14285
3.281287085 0.040727702 0.982 15 97 71
0.103199 27.13810
3.484925597 0.040791459 1.202 17 32 75.125
0.105708 29.85392 71.43478
3.892840186 0.040770521 1.643 25 57 26
0.109769 29.86634 70.54285
4.300545393 0.040763254 2.469 48 26 71
0.116279 29.87065 72.76470
4.708177936 0.040777787 3.711 07 48 59
0.127877 29.86203 71.55128
5.115955809 0.04077426 5.581 24 19 21
0.150150 29.86412 72.32758
5.523698413 0.040772416 8.390 15 4 62
0.203665 29.86521 72.07428
5.931422568 0.040771906 12.613 99 84 57
0.440528 29.86552 71.82575
6.339141631 0.014075028 18.962 63 05 76
0.588235 73.57713 72.43859
6.409516771 0.009262105 8.258 29 73 65
0.769230 110.4861
6.455827296 0.006675527 5.760 77 11 72
152.3148
6.489204931 0.002176563 4.320 1 15 72
1.111111 461.9444
6.500087747 0 1.440 11 44 72
1.111111
6.500087747 0.050000675 0.000 11 #DIV/0! #DIV/0!
Universidad del Valle Guatemala Rodrigo Muralles
Bioingeniera Carnet: 12262

Grficos

Tiempo vs Conc. glucosa

12.00
10.00
8.00
6.00 Tiempo vs Conc.
Conc. Glucosa (mol/L) 4.00 glucosa
2.00
0.00
100.00
0.00 200.00

Tiempo (min)

Grfico 1. Tiempo vs la concentracin de glucosa (mol/L)

Universidad del Valle Guatemala Rodrigo Muralles
Bioingeniera Carnet: 12262

Tiempo vs Conc. Celular (g/L)

700.00
600.00
500.00
400.00
Tiempo (min)
Conc. Celular (g/L) 300.00
200.00
100.00
0.00
0.00 100.00200.00

Tiempo (min)

Grfico 2. Tiempo vs la concentracin celular (g/L)

Velocidad de crecimiento
12

10

6 Velocidad de crecimiento
Tiempo (min)
4

0
0.00 50.00 100.00 150.00

rN

Grfico 3. Velocidad de crecimiento celular

Universidad del Valle Guatemala Rodrigo Muralles
Bioingeniera Carnet: 12262

d ln(CN)/dt
0.06

0.05

0.04

0.03 d ln(CN)/dt
dln(CN)/dt
0.02

0.01

0
0.00 50.00 100.00 150.00

Tiempo (min)

Grfico 4. Velocidad crecimiento especfica

Linealizacin
35
30
f(x) = 1.58x + 21.85
25 R = 0.85
20 linealizacin
CN/rN 15 Linear (linealizacin)
10
5
0
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5

ln(CN)

Grfico 5. Linealizacin

Respuestas

y=mx +b
Universidad del Valle Guatemala Rodrigo Muralles
Bioingeniera Carnet: 12262

Ks 1
y= x +
max max

0.040765639 min-1
max 0.045787546 min-1
Tiempo generacin 17.00322142 min
Yx/s 0.373556888 g/g
Ks 0.072115385 mol/L