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BROMATO SUPERIOR

Curva de calibracin Fluoreno

1/400 (20 ul)

# Time Area Height Width Area% Symmetry

1 4.822 38.3 4.6 0.1329 100.000 0.624


VWD1 A, Wavelength=261 nm (BS2017\BS170008.D)
mAU

4.822
5

-1
0 1 2 3 4 5 6 7 8 9 min

1/40 (20 ul)

# Time Area Height Width Area% Symmetry

1 4.824 368.4 44.8 0.1193 100.000 0.56


VWD1 A, Wavelength=261 nm (BS2017\BS170006.D)
mAU
4.824

40

35

30

25

20

15

10

0 1 2 3 4 5 6 7 8 9 min
1/10 (20ul)

# Time Area Height Width Area% Symmetry

1 4.817 1461.8 177.9 0.1192 100.000 0.561

VWD1 A, Wavelength=261 nm (BS2017\BS170005.D)


mAU

4.817
160

140

120

100

80

60

40

20

0 1 2 3 4 5 6 7 8 9 min

Muestra INCGNITA

# Time Area Height Width Area% Symmetry

1 4.823 527.6 63.8 0.1329 100.000 0.642


VWD1 A, Wavelength=261 nm (BS2017\BS170012.D)
mAU
4.823

60

50

40

30

20

10

0 1 2 3 4 5 6 7 8 9 min
BS 24 Abril 2017
Puntos que faltaban en la curva de calibracin Fluoreno, los volvimos correr:

1/200 (20ul)

# Time Area Height Width Area% Symmetry

1 4.765 82.5 10 0.1331 100.000 0.625


VWD1 A, Wavelength=261 nm (BS2017\BS172001.D)
mAU

4.765
10

0 1 2 3 4 5 6 7 8 9 min

1/100 (20ul)

# Time Area Height Width Area% Symmetry

1 4.769 163.8 19.7 0.1349 100.000 0.609


VWD1 A, Wavelength=261 nm (BS2017\BS172002.D)
mAU
4.769

17.5

15

12.5

10

7.5
1.583

5
1.693
1.728

2.5

0 1 2 3 4 5 6 7 8 9 min
1/50 (20ul)

# Time Area Height Width Area% Symmetry

1 4.757 321.1 38.7 0.1347 100.000 0.608


VWD1 A, Wavelength=261 nm (BS2017\BS172003.D)
mAU

4.757
35

30

25

20

15

10

0 1 2 3 4 5 6 7 8 9 min

1/20 (20ul)

# Time Area Height Width Area% Symmetry

1 4.766 820.8 98.5 0.135 100.000 0.603


VWD1 A, Wavelength=261 nm (BS2017\BS172004.D)
mAU
4.766

80

60

40

20

0 1 2 3 4 5 6 7 8 9 min
Como ejemplo les paso cmo se pueden comparar los distintos cromatogramas
que obtuvimos, superponindolos:

O vindolos por separado uno arriba del otro:

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