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WELCOME

Cell physiology - Class 1 - 2016.09.29


INSTRUCTOR INFORMATION

Dr. Eric Sweet

esweet@wcupa.edu

office: 322 Merion

office hours: Tuesday 9-11 AM, Wednesday 1-3 PM, and Thursday 11-12 PM
SYLLABUS

Essential Cell Biology, 4th edition

Attendance is mandatory

Notify me prior to any unavoidable absence to obtain makeup work

Alert me before the next lecture if you have disabilities requiring accommodation
GRADING

Lecture
Combined Lecture and
Lab 4 exams - 72 posts
each
Lecture 300
Participation and
Lab 150
attendance 12 pts
Total 450
In class and groups
CLASS EXPECTATIONS

Expectations established in class. These are goals that we will strive for a class in addition
to the stated guidelines in the syllabus.

Instructor should give short personal (ungraded) quizzes

Instructor should keep class interactive

Students should speak when answering a question in class

Instructor should go over exam material to help students know what material they are
responsible for
CONCEPT MAP

THE CELL (BIOLOGY)


Form into groups of 6

3 in one row and 3 in the row behind

5 min - write down words on stickies that you think of when you the cell

8 min organize your stickies and make connections

5 min share with the class


CONCEPT MAP
CELLS, MICROSCOPES, AND MODEL
ORGANISMS
CELLS HAVE A WIDE VARIETY OF MORPHOLOGY
ALL CELLS SHARE CERTAIN TRAITS

All cells obey the central dogma and


have DNA, RNA, and proteins

This is the level of detail you are


expected to know for this class

All cells have lipid membranes

All cells arise from pre-existing cells


EUKARYOTIC CELL

Membrane bound
organelles

Typically drawn in
schematic
YOU ARE RESPONSIBLE FOR

Differences between prokaryote and eukaryote

Organelles and their function in the cell eukaryotic cell (pg 15-22)

stop at the cytoplasm is far from static

We will be covering these more specifically in later chapters


MORE COMPLICATED SCHEMATIC
THE CELL IS A CROWDED PLACE
Images made by Dr. David S
Godsell

Illustrated using actual protein


structure and concentrations
found in the cell

Proximity is an important
regulator of molecular
interactions

Molecular interactions can be


Prokaryote outer Eukaryotic neucleus
selective or promiscuous
membrane
David S. Godsell
THE INTERIOR OF CELL IS CONSTANTLY
MOVING

cytoplasmic
streaming in a
plant cell

chloroplasts
dragged along
cytoskeleton by
motor protein
OUTSIDE THE CELLS IS CROWDED TOO

Cells and how they interact is not


entirely the neat and clean
diagrams we will go over in this
class
MODEL ORGANISMS
WHY MODEL SYSTEMS?
SINGLE CELL MODELS
S. cerevisiae - eukaryotic
E. coli - prokaryotic

breeds quickly, cheap


breed quickly, cheap

performs all the basic


central dogma conserved
cellular functions
post translational
has mitochondria
modification of protein
different
many molecular
components conserved
protein production
with humans

secretory pathway
MULTICELLULAR ORGANISMS
C. elegans - Drosophila
melanogaster - fly
nematode
genes controlling
959 cells - we know development similar to
the lineage map humans

easy to find mutation very strange gene


names - Shh, frizzled,
cheap, easy to grow etc

genetics and
apoptosis,
developmental biology
development
PLANTS
Arabadopis thaliana

weed, grows quickly, cheap

closely related to other


flowering species

arabadopsis genes have


counterparts in agricultural
species

microtubules, pesticides
VERTEBRATE MODELS
Zebrafish Rat and mouse

cheap organism, mammalian


expensive tanks
highly conserved
processes
translucent for first 2
weeks of life
breed relatively quickly -
21 day gestation
can absorb many
chemical through the more expensive then non-
skin vertebrates

development/toxicology similar learning behaviors


CELL LINES
Primary or immortalized
human cell lines
primary - taken directly from a living
organism, limited lifespan

immortalized - originally came from a living


organism but can be passaged forever

cheaper then an organism

doesnt have interconnectedness in the body


Mutation in the gene Kit, required
carry unique genetic information of an
individual for maintenance of pigment cells