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reagents
REF 8-500 DILUENT (20 L)
8-501 LYSING REAGENT CN FREE (400 ml)
8-502 LYSING REAGENT CN FREE (6 x 400 ml)
8-503 LYSING REAGENT EO (1 L)
8-504 LYSING REAGENT BASO II (1 L)
8-505 ENZYMATIC CLEANER (1 L)
IVD
SUMMARY
UTILISATION (For In Vitro Diagnostic use)
ABX PENTRA blood counters use the impedance and optical technology DILUENT is a buffered solution which is diluting the blood sample
to measure the number of cells in a diluted blood sample. before analysis.
The dilution is done with an isotonic solution which is a conductor and ENZYMATIC CLEANER is designed to remove protein contaminants
does not lyse the blood cells. from the measurement system analyser after each blood sample
RBC/PLT are counted with impedance variation generated by the analysis. The presence of an enzyme reduces the formation of
passage of cells through a calibrated microaperture. proteins deposit.
HGB is measured spectrophotometrically after lysis of red blood cells.
WBC are counted analogically to the RBC and simultaneously are LYSING REAGENT CN FREE is lysing agent to obtain the
analyzed with techniques based on impedancemetry and optics. spectrophotometrically measurement of the haemoglobin.
Three separate dilutions are prepared for WBC(LMNE), WBC/BASO and LYSING REAGENT EO lyses red blood cells and stabilize the native
for RBC/PLT/HGB. form of white blood cells. It also stains the nuclei of eosinophils.
LYSING REAGENT BASO II breaks down membranes of the all
COLLECTION AND STORAGE leucocytes except basophiles, which are then differentiated.
ABX PENTRA 60, ABX PENTRA 60 C+, ABX PENTRA 80 are a These reagents are the functional set to perform blood sample
multiparameter, automated hematology analysers performing analysis on haematology analyser.
haematological analysis on whole blood collected on EDTA tubes.
For the samples collection and storage please refer to the Operator COMPONENTS
Manual of your instrument. DILUENT LYSING REAGENT CN FREE
sodium / potassium chloride surfactants < 0,5 g/l
UTILISATION < 10g/l quaternary ammonium salt
inorganic phosphate buffer 25 - 30 g/l
Before running the analysis, the sample should be gently mixed. ~ 3,5 g/l
Do not mix the different lots of reagents. sodium sulphate 10 g/l
sodium fluoride < 1 g/l
EDTA < 1 g/l
CONSERVATION AND SHELF LIFE preservative < 3 g/l
The reagents must me stored between 18C and 30C a nd used before ENZYMATIC CLEANER LYSING REAGENT EO
the expiry date indicated on the label. sodium phosphate < 6 g/l sodium Cl / SO4 / CO3 ~ 20 g/l
sodium sulphate < 9 g/l dimethyl oxides < 1 g/l
sodium chloride < 4 g/l dyes < 0.15 g/l
preservative 1 g/l surface active comp. ~ 3 g/l
REFERENCE proteolytic enzymes < 9 g/l alcohols and glycols < 70 g/l
Refer to the Operator manual for the analysers.
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