Documentos de Académico
Documentos de Profesional
Documentos de Cultura
CH3 COOH
Acetic acid is an organic compound with chemical formula and also the main
component of vinegar. In this experiment, the concentration of acetic acid in vinegar can be
determined by performing titration process. In the first experiment, solution of potassium
C8 H 4 O 4
hydrogen phthalate (KHP), KH is being titrated with sodium hydroxide, NaOH
solution. This titration process is repeated two times to get average result. The entire procedure
by which we obtain the molarity of a solution of one substance, NaOH from an accurately known
amount of another substance KHP is called standardization. The average molarity of the NaOH
solution will be used in the next experiment. The second experiment is conducted to determine
CH3 COOH
the molarity of and mass percent in vinegar. 100mL of distilled water was added
to 10mL of vinegar and followed by 1mL of NaOH was poured into the solution. In conclusion,
KHP solution is prepared to standardize the NaOH solution. The molarity of the standardize
NaOH solution is used to determine the molarity and the percentage by mass of the acetic acid in
vinegar. From the experiment, the molarity of NaOH from the titration with KHP solution for
titration 1 is 0.37620M, as for titration 2 is 0.3664M. From this value, the average calculated for
the molarity is 0.3713M. The average molarity of NaOH solution is used as standard molarity for
titration with acetic acid. In titration of NaOH solution with acetic acid solution, the results are
almost the same. The molarity of acetic acid for titration 1 is 0.80883M, while titration 2 is
0.7878M. From these values, the average molarity and percentage by mass of the acetic acid is
calculated. The average molarity is 0.7983M acetic acid while the average of the percent by mass
of acetic acid is 4.79%. Lastly, the percentage error calculated is 0.21%. The experiment was
completely and successfully done.
1
INTRODUCTION
moles of solute
Molarity (M) = litre of solution
Equation - 1
Percent by mass is the mass in grams of solute per 100 grams of solution.
grams of solute
100
Percent solute = grams of solution Equation - 2
Vinegar is a dilute solution of acetic acid. Since vinegar is an acid it can be titrated with a base.
Both molarity and percent by mass of acetic acid in a vinegar solution can be determined by
performing a titration. Titration is the process of adding a known amount of a solution of known
concentration to a known amount of solution of unknown concentration. The purpose of titration
is to determine the equivalence point of the reaction. The equivalence point of a titration occurs
when chemically equivalent amounts of acid and base are present. At this point the pH changes
rapidly with a small addition of acid or base. If a pH meter is used in the titration and the pH
plotted against the volume of base added, the equivalence point is the middle of the vertical part
of the curve. Once the equivalence point of the titration is known, the concentration of the NaOH
can be determined by doing calculations. The NaOH is then used to titrate vinegar, and the
2
CH3 COOH
concentration of the vinegar is determined. The percent by mass of acetic acid,
AIMS
CH 3 COOH
To determine molarity of a solution and percent by mass of acetic acid, in
THEORY
In the titration process, a burette is used to dispense a small, quantifiable increment of solution of
known concentration as shown in figure 1. A typical burette has the smallest calibration unit of
1mL. Therefore, volume dispense from the burette should be estimated to the nearest 0.01mL.
Figure 1: a) Depicts a typical 50-mL burette. b) Indicates smallest calibration unit, 0.1mL, on a
typical 50 mL burette.
3
In this experiment, the equivalence point occurs when the moles of acid in the solution equals to
the moles of base added in the titration. For example, the stoichiometric amount of 1 mole of the
CH3 COOH
strong base, NaOH is necessary to neutralize 1 mole of the weak acid, as
indicated in equation 3.
Equation 3
The sudden change in the pH of the solution shows that the titration has reached the equivalence
point. pH in an aqueous solution is related to its hydrogen ion concentration. Symbolically, the
+
hydrogen ion concentration is written as [ H 3O ]. pH is defined as the negative of the
+
pH = - log [ H 3O ] Equation - 4
pH scale is a method to express the acidity or basicity of a solution. Solutions having a pH < 7
+
are acidic, pH = 7 are neutral, pH > 7 are basic. For example, a solution having [ H 3 O ] =
2
2.35 10 M would have a pH of 1.629 and is acidic. pH electrode will be used in this
experiment. The titration is initiated by inserting a pH electrode into a beaker containing the acid
solution. As NaOH is incrementally added to the acid solution, some of the hydrogen ions will be
neutralized. As the hydrogen ion concentration decreases, the pH of the solution will gradually
increase. When sufficient NaOH is added to completely neutralize the acid, the next drop of
NaOH added will cause a sudden sharp increase in pH. The volume of based required to
completely neutralized the acid is determine at the equivalence point of titration.
4
Figure 2: Acid-base titration curve of weak acid titrated with NaOH
In this experiment, titration of vinegar sample with a standardized NaOH solution will be done.
To standardize the NaOH solution, a primary standard acid solution is initially prepared.
In general, primary standard solutions are produce by dissolving a weighed quantity of pure acid
or base in a known volume of solution. Primary standard acid or bases have several common
characteristics:
C 8 H 4 O4 2
Potassium Hydrogen Phthalate (KHP), KH and oxalic acid, (COOH are
a2 O2
common primary standard acid. Sodium Carbonate, N C , is the most commonly used
based. Most acids and bases are most available as primary standard. To standardize one of these
acid or based solutions, titration of the solution with a primary standard should be done. In this
experiment, NaOH solution will be titrated with KHP. The equation for this reaction will be:
5
C 8 H 4 O4 C8 H 4 O4 H2
KH (aq) + NaOH(aq) KNa (aq) + O(l)
Equation - 5
Once the NaOH solution has been standardizes, it will be used to titrate 10.00mL aliquots of
vinegar. The equation for the reaction of vinegar with NaOH is
CH 3 COOH CH 3 COOH H2
(aq) + NaOH(aq) Na (aq) + O(l)
Equation - 6
Knowing the standardized NaOH concentration and using equation 6, we can determine the
molarity and percent by mass of acetic acid in the vinegar solution.
Figure 3 describes the titration curve of 1.523 grams of KHP dissolved in 20.0mL of distilled
water titrated with NaOH. Determine the molarity of the NaOH solution.
6
Figure 3: Titration curve of KHP with NaOH.
1 mol KH C 8 H 4 O 4
1.523 g KH C 8 H 4 O4 =0.007458 mol KH C 8 H 4 O4
204.2 g KH C 8 H 4 O 4
2. From equation 5, calculate the moles of NaOH required neutralizing the moles of KHP.
1mol NaOH
0.007458 mol KHP =0.007458 mol NaOH
1 mol KHP
1L
15.30 g NaOH =0.01530 L NaOH
100 mL
A 10.00mL aliquot of vinegar requires 16.95mL of the 0.4875M standardized NaOH solution to
reach the equivalence point of the titration.
Sample calculations for determining the acetic acid concentration in vinegar by titration with
standard base and its percent by mass:
7
1L
16.95 mL NaOH =0.01695 L NaOH
1000 mL
1 mol CH 3 COOH
0.008263 mol NaOH =0.008263 mol NaOH
1 mol NaOH
CH 3 COOH
6. Calculate the molarity of the solution
1L
10 mL CH 3 COOH =0.010 L CH 3 COOH solution
1000 mL
M=
1L
10 mL CH 3 COOH =0.010 L CH 3 COOH solution
1000 mL
8
8. Calculate the percent by mass of acetic acid in the solution
g CH 3 COOH
CH 3 COOH 100
Percent mass = g CH 3 COOH solution
0.4693 g of CH 3 COOH
CH 3 COOH 100
Percent mass = 10.00 g CH 3 COOH = 1.963%
CH 3 COOH
1. Funnel
2. 10 cm3 pipette
3. 100 cm3 volumetric flask
4. 100 cm3 beaker or conical flask
5. Retort stand with clamp
6. Electrode with pH meter
7. Analytical balance
8. 50 cm3 Burette
9. Magnetic stirrer
9
PROCEDURE
1. 250 mL of approximately 0.6 M sodium hydroxide solution from NaOH solid is prepared.
The solution is prepared in a beaker, and the calculation is checked with the laboratory
instructor prior to preparing the solution. The calculation is recorded.
2. A 250 mL beaker is weighted and the mass is recorded to the nearest 0.001g. 1.5 grams of
KHP is added to the beaker. The mass of the beaker and KHP is recorded to the nearest
0.001g. The mass of KHP by difference is calculated and the data is recorded. 30mL of
distilled water is added to the beaker. The solution is added until the KHP has dissolved
completely.
3. The solution is titrated with NaOH and the pH with 1 ml additions of NaOH solution is
recorded.
4. Steps 1 to 3 are repeated to perform a second trial to standardize the NaOH solution.
5. The graph of pH versus NaOH is plotted. The volume of NaOH required to neutralize the
KHP solution in each titration is determined for the plots.
7. The average molarity of NaOH solution for titration 1 and 2 is calculated. The resulting
NaOH concentration is used in part B of the experiment.
10
Molarity of acetic acid and percent of vinegar
1. 10.00 mL of vinegar is transferred to a clean, dry beaker using 10mL volumetric pipette.
Sufficient water, 100mL is added to cover the pH electrode tip during titration.
4. The graph of pH versus volume of NaOH added is added and the volume of NaOH
required to neutralize vinegar in each titration is determined from the plots. The data is
recorded.
7. The percent by mass of acetic acid in vinegar for titration 1 and 2 is calculated.
11
RESULT
12
10
8
pH 6
0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22
12
10
8
pH 6
0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22
12
Graph of pH against volume of NaOH titrated with acetic acid (Titration 1)
12
10
6
pH
4
0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
10
6
pH
4
0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
13
CALCULATIONS
MV 0.6 250
n= 1000 = 1000 = 0.15mol
TITRATION 1 TITRATION 2
Mass of beaker (g) 96.7503 98.6740
Mass of beaker + KHP (g) 98.2484 100.1330
Mass of KHP (g) 1.4980 1.4590
Volume of NaOH to neutralize
19.5000 20.0000
the KHP solution (mL)
Titration 1:
1mol KH C8 H 4 O4
n KHP=1.4980 g KH C 8 H 4 O 4 =0.007336 mol KH C 8 H 4 O4
204.2 g KH C 8 H 4 O4
1 mol NaOH
0.007336 mol KHP =0.007336 mol NaOH
1 mol KHP
14
1L
M NaOH =19.50 mL NaOH =0.01950 L NaOH
1000 mL
M 1=
0.37620+ 0.3664 M
M avg
= 2 = 0.3713M NaOH
CH 3 COOH
Standardization of NaOH solution with
TITRATION 1 TITRATION 2
Volume of NaOH to neutralize
(mL)
Titration 1:
1L
21.50 mL NaOH =0.02150 L NaOH
1000 mL
15
Moles of CH 3 COOH neutralized by themoles of NaOH
1mol CH 3 COOH
0.0080883 mol NaOH 1 mol NaOH
=0.0080883mol NaOH
1L
10 mL CH 3 COOH =0.010 L CH 3 COOH solution
1000 mL
M=
1L
10 mL CH 3 COOH =0.010 L CH 3 COOH solution
1000 mL
CH 3 COOH
0.010L
16
1 g CH 3 COOH solution
CH 3 COOH solution
10mL 1 mL CH 3 COOH solution = 10.00g
CH 3 COOH solution
g CH 3 COOH
CH 3 COOH 100
Percent mass = g CH 3 COOH solution
0.485298 g of CH 3 COOH
CH 3 COOH 100
Percent mass = 10.00 g CH 3 COOH = 4.85298%
CH 3 COOH
DISCUSSION
CH 3 COOH CH 3 COOH as
and NaOH. NaOH as a base and acid substance where the
CH 3 COOH
concentration of is unknown.
17
The 250mL 0f 0.6 M of NaOH solution is prepared by weighing approximately 6.0g of NaOH
solid by using analytical balance. The NaOH solid is dissolved in about 100mL of distilled water
inside a beaker. After the solid is dissolved, the solution is poured slowly inside the 250 ml
volumetric flask. The beaker is then rinsed with a small amount of distilled water to ensure all of
the NaOH solution inside the beaker is poured into the volumetric flask. This step is repeated
twice. After that, the volumetric flask is filled with distilled water until it reaches the calibration
mark. To ensure that the distilled water added to the flask is not exceeding the calibration mark,
the distilled water is added slowly and the eyes must be perpendicular to the calibration mark at
reading of burette.
KHP solution is prepared for the standardization of NaOH. In preparation of KHP solution,
approximately 1.4980g of KHP is measured and dissolved completely with 250mL of distilled
water in a beaker. The solution is then poured into a 250mL conical flask for neutralization
process. This solution is used for the first titration with NaOH solution. A pH meter is used to
measure the pH value. The initial pH value of KHP solution is 3.63 which is acidic. During the
titration, an interval of 1ml NaOH solution is added to KHP solution to record the increase in pH
value. The titration between NaOH solutions and KHP solutions had given an equivalence point
at pH 8 after 19.5mL of NaOH solution added. The equivalence point can be obtained from the
graph which is located at the center point of the almost straight line graph.
The graph of this titration shows a slow increase at first and when it reaches the equivalence
point, the graph increases rapidly. At 19.5mL of NaOH added, the KHP solution is neutralized
and this volume is used to calculate the molarity of the standardized NaOH solution. Molarity of
NaOH solution can be calculated by dividing the number of moles of NaOH and the volume of
NaOH solution at equivalence point in liter. The molarity of NaOH for this titration is 0.3762M.
Then, the second KHP solution is prepared for the second titration. The titration is repeated to
ensure that the result obtain is more precise and accurate. The equivalence point for the second
titration is at pH 8 and the volume used to neutralize the NaOH solution is 19.5mL. The graph of
the second titration is nearly the same as the first titration, thus it shows that the experiment is
almost precise. The molarity that is obtained from the second titration is 0.3664M. Thus, this
gives average molarity of 0.3713M for the standardized NaOH solution.
18
The average molarity from the standardized NaOH solution is used to determine the molarity of
acetic acid in vinegar and their percentage by mass. A 10mL of vinegar solution is measured by
using volumetric pipette and the solution is transferred into a beaker. A volume of 100mL
distilled water is added into the beaker to ensure that the tip of the pH electrode is immersed
inside the solution during the titration. The solution is poured into a conical flask. This solution
is titrated with NaOH solution and is labeled as the first titration.
During the titration, an interval of 1 ml of NaOH solution is added into the conical flask and the
pH electrode is immersed inside the solution. The pH value is recorded at each interval and the
graph is plotted. The graph shows a slightly increase from the initial pH that is 2.09 and when it
reaches the equivalence point at pH 7.6, the graph shows a gradually increase. The volume of
NaOH used to neutralize the acetic acid is 21.5mL. The volume is used to calculate the molarity
of the acetic acid in the vinegar solution. The molarity of acetic acid for titration 1 is 0.80883M.
The experiment is repeated to obtain an accurate reading of the pH value and volume of NaOH
used. As for the second titration of vinegar solution, the result obtained is 0.7878M.The average
molarity of acetic acid for the titration is 0.7983M.
Percentage by mass is calculated by dividing the mass of acetic acid and the mass of the acetic
acid solutions. Then, multiply it by 100% to get the percentage. For titration 1, the mass of the
acetic acid is calculated by using the molarity formula. Firstly, the number of mole is calculated
by multiplying 0.80883M of acetic acid and the volume of acetic acid solution, 10mL. Hence, by
using the formula mass equals to number of mole multiply by the molar mass of acetic acid, the
mass of ac5etic acid is obtain which is 0. 485298 g. Then, the mass of acetic acid solution,
vinegar, can be obtained by converting 10mL to 10g. Lastly, the percent by mass can be
calculated by dividing 0.485298g with 10g and then multiply by 100%. The percentage by mass
of acetic acid for titration 1 is 4.85298% for the second titration the mass of acetic acid is
0.4727g and the percentage is 4.727%. The average of the percent of acetic acid is 4.79%.
According to a theory, vinegar contains about 5% of acetic acid by volume which making it one
of the main component other than water. The percentage error is calculated to ensure that the
experimental value that is obtained does not show high differences in value. Thus, the percentage
19
error that is calculated is 0.21%. The percentage error shows that the experimental result and the
actual result are less accurate. This is due to misreading of burette
CONCLUSION
In conclusion, KHP solution is prepared to standardize the NaOH solution. The molarity of the
standardize NaOH solution is used to determine the molarity and the percentage by mass of the
acetic acid in vinegar. From the experiment, the molarity of NaOH from the titration with KHP
solution for titration 1 is 0.37620M, as for titration 2 is 0.3664M. From this value, the average
calculated for the molarity is 0.3713M. The average molarity of NaOH solution is used as
standard molarity for titration with acetic acid. In titration of NaOH solution with acetic acid
solution, the results are almost the same. The molarity of acetic acid for titration 1 is 0.80883M,
while titration 2 is 0.7878M. From these values, the average molarity and percentage by mass of
the acetic acid is calculated. The average molarity is 0.7983M acetic acid while the average of
the percent by mass of acetic acid is 4.79%. Lastly, the percentage error calculated is 0.21%.
RECOMMENDATION
There are some recommendations for this experiment. First, we must ensure that there are no
bubbles trapped at the tip of the burette during the filling of NaOH solution because the air
bubble in the burette can cause error in the true value of NaOH used. Second, we must avoid
from making parallax error for most of the measured content. Make sure to take the reading of
meniscus from eye level to ensure accuracy of measurement. Third, the analytical balance could
give wrong reading because of the disturbance. Hence, make sure to close the glass door of the
analytical balance so that any disturbance such as air does not affect the reading. Fourth, make
sure you are wearing goggle and gloves as you are handling chemicals that could be corrosive
and dangerous. Be cautious when handling the sodium hydroxide base, NaOH as it is corrosive
and can cause chemical burns to the skin. Rinse the chemical immediately under running water
for several minutes if it is in contact with your skin. Report the accident to your instructor.
20
REFERENCE
1. Acetic Acid. (2011, October 8). Retrieved October 9, 2011, from Wikipedia:
http://en.wikipedia.org/wiki/Acetic_acid
2. Bobby Stanton, Lin Zhu, Charles Atwood, 2010, Experiment in General Chemistry;
Featuring Measure Net; Determination of the concentration of acetic acid in vinegar, 2 nd
edition, Cancage Editor
3. Determination of acetic acid in vinegar. Available at:
http://linus.chem.ku.edu/genchemlab/184FA06/Download184_Labs/Vinegar
%20Download.htm (Accessed on 28th March 2014)
4. Measuring the amount of Acetic Acid in Vinegar. Available at:
http://projects.icbse.com/chemistry-327 (Accessed on 28th March 2014)
5. Albert R. Leeds, 1895, Journal of The American Chemical Society, Acetic Acid in
Vinegar, ACS Publication
APPENDICES
Titration 2:
21
The moles of KHP used in the titration
1mol KH C8 H 4 O4
n KHP=1.4590 g KH C 8 H 4 O 4 =0.007145mol KH C8 H 4 O4
204.2 g KH C 8 H 4 O4
1mol NaOH
0.007145 mol KHP =0.007145 mol NaOH
1 mol KHP
1L
M NaOH =19.50 mL NaOH =0.01950 L NaOH
1000 mL
M 2=
CH 3 COOH
Calculation of standardization of NaOH solution with for:
22
Titration 2:
1L
21.50 mL NaOH =0.02150 L NaOH
1000 mL
1mol CH 3 COOH
0.007878 mol NaOH 1 mol NaOH
=0.007878mol NaOH
1L
10 mL CH 3 COOH =0.010 L CH 3 COOH solution
1000 mL
M=
23
1L
10 mL CH 3 COOH =0.010 L CH 3 COOH solution
1000 mL
CH 3 COOH
0.010L
1 g CH 3 COOH solution
CH 3 COOH solution
10mL 1 mL CH 3 COOH solution = 10.00g
CH 3 COOH solution
g CH 3 COOH
CH 3 COOH 100
Percent mass = g CH 3 COOH solution
0.4727 g of CH 3 COOH
CH 3 COOH 100
Percent mass = 10.00 gCH 3 COOH = 4.727%
CH 3 COOH
24
25