Application:

1. Trace sample analysis

2. PCR - Karyotyping
3. Forensic examination
4. Genetically modified organisms detection

Note:
1. Since Rally Taq is a highly efficient enzyme,
Description: we recommended the reaction mix should
2 Rally PreMix is an optimized and ready-to-use keep on ice before PCR amplification started.
mixture contains of reaction buffer, dNTPs, loading 2. To avoid non-specific amplification caused
dye, glycerol, PCR enhancers and the PCR by the superb activity of Rally Taq, the ideal
polymerase specially engineered by TEN GIGA BIO amplification target is around 1 kb.
(Rally Taq) as 2-fold concentration. Comparing with
conventional Taq polymerase, Rally Taq exhibits Reaction Mixture for Recommended PCR assay:
extremely high reaction efficiency and is able to
amplify DNA fragments from 1~10 copies of Component Amount per reaction
template. In addition, our Rally Taq is also capable Raw material X
to resist most PCR inhibitors which are commonly 10 M forward Primer 0.5 l
found in blood and plant tissues. In combination
10 M reverse Primer 0.5 l
with our specially optimized buffer system, 2 Rally
2X Rally PreMix 12.5 l
PreMix is suitable for PCR amplification directly
Nuclease-free water to 25 l
from whole blood, and samples from cell lines or
Total volume 25 l
tissues. You may skip any prior DNA purification
steps, only applying a tiny amount of raw material
directly into the PCR reaction, running in a PCR Thermocycling Conditions for a Routine PCR:
machine, and directly loading into an agarose gel for
Segment Number Step Temperature Time
electrophoresis.
of cycles

Instruction of using whole blood as templates: 1 1 Initial 95oC 10 minutes

Heparin, EDTA or citrate treated whole blood denaturation
sample is all suitable for Rally PreMix. Although 1-10 2 35-40 Denaturation 95oC 30 seconds
% of blood in the final PCR reaction is recommended, Annealing Primer Tm 30 seconds
DNA amplification can be achieved in the present of + 3-5OC
~ 25% blood. Extension 72oC 10-15
sec/kb
Instruction of using plant tissues as templates: 3 1 Final 72oC 5-10
By using puncher or other tools, plant tissue with 0.5 Extension minutes
mm in diameter is able to add directly into a 25 L
Hold 14oC -
PCR reaction without pre-treatment. The
recommended size of plant tissue is <1 mm in
diameter.