k T/Kelvin lnk 1/T kcalc

1.69E-05 298.15 -10.9881969 0.00335402 1.718127E-005

6.73E-05 308.15 -9.60635032 0.00324517 6.667983E-005
2.49E-04 318.15 -8.29805766 0.00314317 0.0002376353
7.50E-04 328.15 -7.19543735 0.00304739 0.0007837716
2.43E-03 338.15 -6.01986402 0.00295727 0.0024088746
-12459
30.816
2.416678E+013

Ea 103590.3555
A 1668E+013
(kcal-ki)^2
7.9112438754164E-014
3.8460533313434E-013
1.2915536737550E-010
1.14052E-09
4.4628071074773E-010
1.7164179065313E-009
0
0.0028 0.003 0.0032 0.0034
-2
-4
Column D
-6
f(x) = - 12458.657497114xLinear (Column D)
+ 30.8157521654
-8
R = 0.99970198
-10
-12
[S] (mM) Control I a 6 nM X a 30 Nm
0.2 16.7 6.25 5.56
0.25 20 7.69 6.67
0.333 24.98 10 8.33
0.5 33.33 14.29 11.11
1 50 25 16.67
2 66.67 40 22.22
2.5 71.4 45.45 23.81
3.33 76.92 52.69 25.66
4 80 57.14 26367
5 80.33 62.5 27.77

[S] (nM) Control I a 6 nM X a 30 Nm

200000 16.7 6.25 5.56
250000 20 7.69 6.67
333000 24.98 10 8.33
500000 33.33 14.29 11.11
1000000 50 25 16.67
2000000 66.67 40 22.22
2500000 71.4 45.45 23.81
3330000 76.92 52.69 25.66
4000000 80 57.14 26.367
5000000 80.33 62.5 27.77
Y a 4 mM Z a 0.2 mM Cambia
10 8.89 Cambia
11.11 10.81 Cambia
12.5 13.78
14.29 19.05
16.67 30.77
18.18 44.44
18.52 48.78
18.57 54.06
19 57.14
19.23 60.6

Y a 4 mM Z a 0.2 mM 1/S 1/VC 1/VI 1/x

10 8.89 0.000005 0.05988024 0.16 0.17985612
11.11 10.81 0.000004 0.05 0.13003901 0.14992504
12.5 13.78 3.0030E-006 0.04003203 0.1 0.12004802
14.29 19.05 0.000002 0.030003 0.06997901 0.090009
16.67 30.77 0.000001 0.02 0.04 0.059988
18.18 44.44 0.0000005 0.01499925 0.025 0.0450045
18.52 48.78 0.0000004 0.0140056 0.0220022 0.04199916
18.57 54.06 3.0030E-007 0.01300052 0.01897893 0.03897116
19 57.14 0.00000025 0.0125 0.01750088 0.0379262
19.23 60.6 0.0000002 0.01244865 0.016 0.03601008
V max Km
NO SI COMPETITIVA
SI NO NO COMPETITIVA
SI SI A COMPETITIVA

1/y 1/z 0.18

0.1 0.11248594 0.16
f(x) = 30000.
0.090009 0.09250694 0.14
0.08 0.07256894 0.12
0.1
0.06997901 0.05249344
0.08
0.059988 0.03249919
0.06
0.0550055 0.02250225 0.04 f(x) = 9962.1
0.05399568 0.02050021 0.02
0.0538503 0.01849797 0
0 0.00000
0.05263158 0.01750088
0.05200208 0.01650165
 V MAX km Tipo
CONTROL 99.009901 986346.535
INHIBIDOR 100 3000000 Competitiva
X 33.32 9.99* 10 5 No competitiva
Y 19.99 1.99 * 10 5 Acompetitiva
Z 80 1.59 * 10 6 Competitiva

Control vs inhibidor
0.18
0.16
0.14 f(x) = 30000.0205857563x + 0.0099900585
0.12
0.1
0.08
0.06
0.04 f(x) = 9962.1359181262x + 0.0100966816
0.02
0
0 0.000001 0.000002 0.000003 0.000004 0.000005 0.000006

CONTROL Linear (CONTROL)

INHIBIDOR Linear (INHIBIDOR)

Control vs x
0.2
0.18
0.16
0.14
0.12
0.1
0.08
0.06
0.04
0.02
0
0 0.000001 0.000002 0.000003 0.000004 0.000005 0.000006

Column J Column L
ompetitiva
mpetitiva

1/ inter y pend * v max

V max Km
I 100 3000000
C 99.009901 986346.535

0.000006

0.000006
 T/s
0
100
200
300
400
600
1000

0.7

0.6

0.5
Ca/moldm3
0.4

0.3

0.2

0.1

0
0 200 40

0
0 200 400

-0.5

Ln (Ca) -1

-1.5

-2

-2.5
 [A]/[Ao] Ca/moldm3 LnCa Ca cal (Ca-Ca cal)^2
1 0.6 -0.51082562 #VALUE! #VALUE!
0.829 0.4974 -0.69836075 #VALUE! #VALUE!
0.688 0.4128 -0.88479206 #VALUE! #VALUE!
0.597 0.3582 -1.02666379 #VALUE! #VALUE!
0.511 0.3066 -1.18221131 #VALUE! #VALUE!
0.385 0.231 -1.46533757 #VALUE! #VALUE!
0.248 0.1488 -1.90515216 #VALUE! #VALUE!
#VALUE!

Orden 0
7

0
0 200 400 600 800 1000 1200

T/s

Orden 1

200 400 600 800 1000 1200

T/s
Cao 0.60008901
k 0.00254194
n 1.47816262
 DATOS DEL PROBLEMA DATOS PARA GRAFICAR
[S] Velocidad [S] 1/[S] 1/Velocidad
M nmol/(L*min) nM 1/Nm nmol/(L*min)
0.045
0.0000025 24 2500 0.0004 0.0416666667
0.04
0.00000333 30 3330 0.0003003 0.0333333333 f(x) = 8
0.000004 34 4000 0.00025 0.0294117647 0.035
0.000005 40 5000 0.0002 0.025 0.03
0.00001 60 10000 0.0001 0.0166666667 0.025
0.00002 80 20000 0.00005 0.0125 0.02
0.00004 96 40000 0.000025 0.0104166667
0.015
0.0001 109 100000 0.00001 0.0091743119
0.01
0.002 119 2000000 0.0000005 0.0084033613
0.005
0.01 120 10000000 0.0000001 0.0083333333
0
0 0.0
 1/V = Km/ V max*(1/S)
+1/Vmax
Vmax 120.481928 1/0.0083
Km 10053.494 83.444*(1/0.0083)

Linew eaver-Bork
0.045
0.04 f(x) = 83.4440491308x + 0.0083433174
0.035
0.03
0.025
0.02
0.015
0.01
0.005
0
0 0.00005 0.0001 0.00015 0.0002 0.00025 0.0003 0.00035 0.0004 0.00045
 "v= -Km *
Vmax"
Vmax
Km

Velocidad V/S
nmol/(L*min) Woof - Aoustin
24 0.0096 0.012
30 0.00900901
34 0.0085 0.01
40 0.008 f(x) = - 9.9877191728
0.008
60 0.006
80 0.004 0.006
96 0.0024
109 0.00109 0.004
119 0.0000595
0.002
120 0.000012
0
0 20 40 60
"v= -Km * (V/S) +
Vmax"
0.012
1.001328

Woof - Aoustinsson- - Hofstee

f(x) = - 9.98771917283921E-005x + 0.011978307

40 60 80 100 120 140

Inciso C

V= (V max * S)/(Km+S) = (V max /Km) * S =

k= V max /Km
0.0119840851
K*S
 DATOS DEL PROBLEMA
S/M V/(nM/min) V/(nM/min)
0.0000002 0.10708943 10708.942548
0.000063 26.7416079 2674160.7921
0.0001 37.8277647 3782776.4706
0.002 114.834286 11483428.571
0.05 128 12800000

Km 0.00024

V max = [V*Km + V[S]]/[S]

1.28614E-007 M/min
128.6144 nM/minincrementada 5 orden

V=( Vmax [S])/(Km + [S])

 V/(M/min)

1.2800E-007

12861440 nM/min