BIOL 2104 (CRN 81024) Fall 2014

CELL & MOLECULAR BIOLOGY: Quiz No. 2, 30 September 2014

Name (print) _________________________ Student Number _____________________

I pledge that the answers on this quiz are the sole efforts of myself. Violations of
this pledge will be adjudicated by the Virginia Tech Honor System.

Include on Opscan!
Test Version A Signature _______________________________

Please provide the most accurate answer for the following questions and
statements.
Jim Tokuhisa 9/26/2015 15:15
Comment [1]: This quiz was pretty
1. Which of the following RNAs has a translator activity? challenging with many questions involving
A. mRNA applications of concepts.
B. miRNA
C. rRNA
D. siRNA
E. tRNA


2. If a gene has a TATA box, would you expect the promoter to bind a sigma
factor?
Jim Tokuhisa 9/26/2015 15:04
A. Yes
Comment [2]: I like to compare processes
B. No between prokaryotes and eukaryotes.


3. You have sequenced the DNA of a virus that is pathogenic to humans. In the
DNA you find several genes that have the promoter and terminator structure
of a eukaryotic gene. Is this a real finding or is the virus DNA contaminated
with human genes? What do you do?
Jim Tokuhisa 9/26/2015 15:09
A. Redo the genome sequencing with a new batch of DNA because the
Comment [3]: Recall and apply the viral
first DNA preparation was contaminated with eukaryotic DNA. reproductive cycle and behavior.
B. Try to relate the gene sequence to the pathogenicity of the virus.

4. Which feature in eukaryotic gene expression definitively prevents translation
occurring simultaneously with transcription?
A. a nuclear envelope
B. a ribosome binding site
C. a TATA box
D. introns
E. initiation factors
F. stop codon

BIOL 2104 (CRN 81024) Fall 2014
CELL & MOLECULAR BIOLOGY: Quiz No. 2, 30 September 2014


5. What can some eukaryotic transcriptional regulators do that prokaryotic
regulators cannot do?
A. bind DNA
B. function as repressors
C. interact with chromatin
D. interact with RNA polymerase

6. Based on germ line cell formation, higher plants are (A) more likely than (B)
less likely than or (C) equally likely as metazoan organisms to pass
environmentally-induced mutations to their progeny.

7. Mitochondria and chloroplasts have there own DNA genomes. Based on their
internal organization they are predicted to have a (A) prokaryotic or (B)
eukaryotic type of translation.

8. Mutations in the coding region of a gene are most likely to alter (A) levels of
gene expression, (B) location of gene expression, or (C) specific activity of the
gene product.

9. If a mutation that causes a neutral genetic change is very close, say 2000 base
pairs, away from a mutation that causes reduced fitness, the frequency of the
neutral genetic change will most likely (A) increase, (B) decrease, or (C)
remain the same in a breeding population.

10. Which enzyme allows researchers to cut double-stranded DNA at precise
sequence-specific locations?
A. DNA ligase
B. restriction nuclease
C. reverse transcriptase
D. Taq polymerase

BIOL 2104 (CRN 81024) Fall 2014
CELL & MOLECULAR BIOLOGY: Quiz No. 2, 30 September 2014

Name (print) _________________________ Student Number _____________________

I pledge that the answers on this quiz are the sole efforts of myself. Violations of
this pledge will be adjudicated by the Virginia Tech Honor System.

Include on Opscan!
Test Version B Signature _______________________________

Please provide the most accurate answer for the following questions and
statements.

1. Which of the following RNAs has a translator activity?
A. tRNA
B. siRNA
C. rRNA
D. mRNA
E. miRNA


2. If a gene has a polyadenylation signal, would you expect the promoter to bind
a sigma factor?
A. Yes
B. No


3. You have sequenced the DNA of a virus that is pathogenic to humans. In the
DNA you find several genes that have the promoter and terminator structure
of a eukaryotic gene. What do you do?
A. Redo the genome sequencing with a new batch of DNA because the
first DNA preparation was contaminated with eukaryotic DNA.
B. Try to relate the gene sequence to the pathogenicity of the virus.

4. Which property of eukaryotic genes prevents translation occurring
simultaneously with transcription?
A. stop codon
B. introns
C. initiation factors
D. a TATA box
E. a ribosome binding site

BIOL 2104 (CRN 81024) Fall 2014
CELL & MOLECULAR BIOLOGY: Quiz No. 2, 30 September 2014


5. What can some eukaryotic transcriptional regulators do that prokaryotic
regulators cannot do?
A. interact with RNA polymerase
B. interact with chromatin
C. function as repressors
D. bind DNA

6. Based on germ line cell formation, higher plants are (A) less likely than (B)
more likely than or (C) equally likely as metazoan organisms to pass
environmentally-induced mutations to their progeny?

7. Mitochondria and chloroplasts have there own DNA genomes. Based on their
internal organization they are predicted to have a (A) eukaryotic or (B)
prokaryotic type of translation.

8. Mutations in the coding region of a gene are most likely to alter (A) location
of gene expression, (B) levels of gene expression, or (C) specific activity of the
gene product.

9. If a mutation that causes a neutral genetic change is very close, say 2000 base
pairs, away from a mutation that causes reduced fitness, the frequency of the
neutral genetic change will (A) decrease (B) increase, or (C) remain the same
in a breeding population.

10. Which enzyme allows researchers to cut double-stranded DNA at precise
sequence-specific locations?
A. Taq polymerase
B. reverse transcriptase
C. restriction nuclease
D. DNA ligase