ALEJANDRO MEJA VILLA

MATRCULA: 2153068647
LABORATORIO DE BIOQUMICA

SECUENCIAS DE AMINOCIDOS Y MTODOS DE

PURIFICACIN
1. ALEMEVIRHCPTWACGTWYP

Posiblemente la cadena de aminocidos tendr carga negativa (-1), un punto

isoelctrico en un medio cido de 3.4 y otro en un medio bsico de 7.8.
Adems de que tendr un peso molecular de ms o menos 2500.

Amino acid composition:

Ala (A) 2 10.0%
Arg (R) 1 5.0%
Asn (N) 0 0.0%
Asp (D) 0 0.0%
Cys (C) 2 10.0%
Gln (Q) 0 0.0%
Glu (E) 2 10.0%
Gly (G) 1 5.0%
His (H) 1 5.0%
Ile (I) 1 5.0%
Leu (L) 1 5.0%
Lys (K) 0 0.0%
Met (M) 1 5.0%
Phe (F) 0 0.0%
Pro (P) 2 10.0%
Ser (S) 0 0.0%
Thr (T) 2 10.0%
Trp (W) 2 10.0%
Tyr (Y) 1 5.0%
Val (V) 1 5.0%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%

(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%

Molecular weight: 2363.75

Theoretical pI: 5.40

Total number of negatively charged residues (Asp + Glu): 2

Total number of positively charged residues (Arg + Lys): 1
 Formula: C107 H155 N27 O28 S3
Total number of atoms: 320

2. YFRGHRYUCTTRDSCVBAWQ

Posiblemente el polipptido tenga una carga positiva de +1, un punto

isoelctrico en un medio bsico de 7.6. Un peso molecular de
aproximadamente 2200.
Molecular weight: 2514.20

Theoretical pI: 8.90

Amino acid composition:

Ala (A) 1 5.0%
Arg (R) 3 15.0%
Asn (N) 0 0.0%
Asp (D) 1 5.0%
Cys (C) 2 10.0%
Gln (Q) 1 5.0%
Glu (E) 0 0.0%
Gly (G) 1 5.0%
His (H) 1 5.0%
Ile (I) 0 0.0%
Leu (L) 0 0.0%
Lys (K) 0 0.0%
Met (M) 0 0.0%
Phe (F) 1 5.0%
Pro (P) 0 0.0%
Ser (S) 1 5.0%
Thr (T) 2 10.0%
Trp (W) 1 5.0%
Tyr (Y) 2 10.0%
Val (V) 1 5.0%
Pyl (O) 0 0.0%
Sec (U) 1 5.0%

(B) 1 5.0%
(Z) 0 0.0%
(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 1

Total number of positively charged residues (Arg + Lys): 3

3. ACCVBMWQRT YGTTKLPOII
4.
5. La cadena de aminocidos tendr un punto isoelctrico en un medio
cido de 6.3. Un peso molecular de 2700. Y una carga negativa de -2
6. Molecular weight: 2436.50
7.
8. Theoretical pI: 8.91
9.
11. Amino acid composition:
12. Ala (A) 1 5.0%
13. Arg (R) 1 5.0%
14. Asn (N) 0 0.0%
15. Asp (D) 0 0.0%
16. Cys (C) 2 10.0%
17. Gln (Q) 1 5.0%
18. Glu (E) 0 0.0%
19. Gly (G) 1 5.0%
20. His (H) 0 0.0%
21. Ile (I) 2 10.0%
22. Leu (L) 1 5.0%
23. Lys (K) 1 5.0%
24. Met (M) 1 5.0%
25. Phe (F) 0 0.0%
26. Pro (P) 1 5.0%
27. Ser (S) 0 0.0%
28. Thr (T) 3 15.0%
29. Trp (W) 1 5.0%
30. Tyr (Y) 1 5.0%
31. Val (V) 1 5.0%
32. Pyl (O) 1 5.0%
33. Sec (U) 0 0.0%
34.
35. (B) 1 5.0%
36. (Z) 0 0.0%
37. (X) 0 0.0%
39.
40.
41. Total number of negatively charged residues (Asp + Glu): 0
42. Total number of positively charged residues (Arg + Lys): 2
43.
44. FGDHWWQTTVBBVVPOYWWT
45.
46. La cadena tendr una carga negativa de -2. Un punto isoelctrico en
un medio cido de 3.5. Un peso molecular alrededor de 3000
47. Molecular weight: 2675.07
48.
49. Theoretical pI: 5.08
50.
52. Amino acid composition:
53. Ala (A) 0 0.0%
54. Arg (R) 0 0.0%
55. Asn (N) 0 0.0%
56. Asp (D) 1 5.0%
 57. Cys (C) 0 0.0%
58. Gln (Q) 1 5.0%
59. Glu (E) 0 0.0%
60. Gly (G) 1 5.0%
61. His (H) 1 5.0%
62. Ile (I) 0 0.0%
63. Leu (L) 0 0.0%
64. Lys (K) 0 0.0%
65. Met (M) 0 0.0%
66. Phe (F) 1 5.0%
67. Pro (P) 1 5.0%
68. Ser (S) 0 0.0%
69. Thr (T) 3 15.0%
70. Trp (W) 4 20.0%
71. Tyr (Y) 1 5.0%
72. Val (V) 3 15.0%
73. Pyl (O) 1 5.0%
74. Sec (U) 0 0.0%
75.
76. (B) 2 10.0%
77. (Z) 0 0.0%
78. (X) 0 0.0%
80.
81.
82. Total number of negatively charged residues (Asp + Glu): 1
83. Total number of positively charged residues (Arg + Lys): 0
84.
85.
86. GTTCCAAGCATGCTAGCGTT
87. Esta cadena tendra una carga positiva de +1, un punto isoeltrico en
un medio cido. Y un peso molecular de 2800
88. Molecular weight: 1709.91
89.
90. Theoretical pI: 5.49
91.
93. Amino acid composition:
94. Ala (A) 4 20.0%
95. Arg (R) 0 0.0%
96. Asn (N) 0 0.0%
97. Asp (D) 0 0.0%
98. Cys (C) 5 25.0%
99. Gln (Q) 0 0.0%
100. Glu (E) 0 0.0%
101. Gly (G) 5 25.0%
102. His (H) 0 0.0%
103. Ile (I) 0 0.0%
104. Leu (L) 0 0.0%
105. Lys (K) 0 0.0%
106. Met (M) 0 0.0%
107. Phe (F) 0 0.0%
108. Pro (P) 0 0.0%
109. Ser (S) 0 0.0%
110. Thr (T) 6 30.0%
111. Trp (W) 0 0.0%
112. Tyr (Y) 0 0.0%
 113. Val (V) 0 0.0%
114. Pyl (O) 0 0.0%
115. Sec (U) 0 0.0%
116.
117. (B) 0 0.0%
118. (Z) 0 0.0%
119. (X) 0 0.0%
121.
122.
123. Total number of negatively charged residues (Asp + Glu): 0
124. Total number of positively charged residues (Arg + Lys): 0
125.
126. Atomic composition:
127.
128. Carbon C 61
129. Hydrogen H 104
130. Nitrogen N 20
131. Oxygen O 27
132. Sulfur S 5
133.
134. Formula: C61H104N20O27S5
135.
136.