Cromatograma M1

1 uL
Pico
tr (min)
Max 1
Sh
Max absorbancia
Sh2
1
10.487
238.5
305
326.3
2
10.897
297.1
3
11.207
244.5
305
326.4
4
11.533
241.6
305
326.2
5
11.683
****
260
6
12.207
245.9
305
323.5
7
12.99
240;305
326.8
8
13.187
****
250-260
9
13.297
251
305
328.2
10
13.44
243.1 ****
268.6
11
13.603
254
355
359.6
12
13.783
254.3
355
360.2
13
14.03
253.8 *****
367.6
14
14.09
268.6
310
351.1
15
14.217
257.4
354.9
16
14.363
257.3
354.4
17
14.91
264.9
350.3
18
17.23
268.2
315.6 Posible
hidroxicinnamicos
Flavonoles
Elagetaninas
Elagicos
Derivado de galico

325nm
355 nm
254 nm
355 nm
280 nm

Pendiente

285

383
365
365
365
dimero de cinnam

Sh3

375
375
375
ible dimero de cinnamico

Cromatograma
1 (M1-1uL)

Elagetaninas
Pico
tr
5
8

255 nm
rea mAU*miRel % area Masa (mg)
11.687
2.14
1.47
49.9
13.187
10.122
6.95
Sumatoria

2 (M22)

5
8

11.677
13.18

2.145
11.367

1.53
8.1

49.4
49.4

3 (M-8)

5
8

11.673
13.177

2.946
14.047

1.91
9.11

51.7
51.7

4 (M-25)

5
8

11.673
13.173

3.083
14.824

1.99
9.73

50.6
50.6

5 (M-13)

5
8

11.67
13.17

2.455
12.755

1.8
9.35

51.1
51.1

6 (M-9)

5
8

11.663
13.167

2.04
10.642

1.58
8.23

50.8
50.8

7 (M-17)

5
8

11.663
13.173

2.414
11.243

1.69
7.86

51.2
51.2

8 (M-18)

5
8

11.667
13.17

2.691
14.143

1.81
9.52

50.6
50.6

9 (M-2)

5
8

11.667
13.167

2.694
13.815

1.83
9.41

50.5
50.5

10 (M-24)

5
8

11.67
13.173

2.539
9.34

1.95
7.17

51.2
51.2

11 (M-7)

5
8

11.67
13.167

1.899
9.044

1.47
6.98

50.7
50.7

12 (M-19)

5
8

11.673
13.163

2.425
13.255

1.7
9.27

51.2
51.2

13 (M-21)

5
8

11.663
13.163

2.422
8.788

1.94
7.04

50.5
50.5

14 (M-16)

5
8

11.66
13.167

2.565
12.913

1.87
9.43

51.3
51.3

15 (M-10)

5
8

11.657
13.167

1.868
4.455

1.6
3.82

50.2
50.2

16 (M-20)

5
8

11.663
13.163

2.703
10.231

1.79
6.77

50.9
50.9

17 (M-3)

5
8

11.667
13.163

2.726
13.464

1.75
8.63

49.2
49.2

18 (M-11)

5
8

11.66
13.17

1.685
2.034

1.81
2.19

52.6
52.6

19 (M-15

5
8

11.667
13.163

2.713
13.725

1.9
9.62

51
51

20 (M-5)

5
8

11.67
13.16

2.9
14.234

1.94
9.53

50.1
50.1

21 (M-12)

5
8

11.667
13.163

2.766
13.881

1.92
9.64

50.9
50.9

22 (M-6)

5
8

11.657
13.16

3.046
15.303

1.9
9.55

49.8
49.8

23 (M-14)

5
8

11.657
13.157

2.33
7.609

1.96
6.4

49.7
49.7

24 (M-23)

5
8

11.66
13.157

2.906
11.505

1.9
7.52

50
50

25 (M-1)

5
8

11.65
13.157

2.864
13.953

1.94
9.43

49.9
49.9

27 (M-4)

5
8

11.653
13.163

2.834
13.729

1.92
9.29

49.8
49.8

Area mAU*min/ g muestra

42.8857715431
202.8456913828

43.4210526316
230.1012145749

56.9825918762
271.7021276596

60.9288537549
292.9644268775

48.0430528376
249.6086105675

40.157480315
209.4881889764

47.1484375
219.58984375

Sumatoria
1 245.731463
2 273.522267
3 328.68472
4 353.893281
5 297.651663
6 249.645669
7 266.738281
8 332.687747
9 326.910891
10 232.011719
11 215.838264
12
306.25
13 221.980198
14
301.7154
15 125.956175
16 254.10609
17 329.065041
18 70.7034221
19 322.313725
20 341.996008
21 327.053045
22 368.453815
23 199.979879
24
288.22
25 337.014028
26 332.590361

53.1818181818
279.5059288538

53.3465346535
273.5643564356

49.58984375
182.421875

37.4556213018
178.382642998

47.36328125
258.88671875

47.9603960396
174.0198019802

50
251.7153996101

37.2111553785
88.7450199203

53.1041257367
201.0019646365

55.406504065
273.6585365854

32.0342205323
38.6692015209

53.1960784314
269.1176470588

57.8842315369
284.1117764471

54.3418467583
272.7111984283

61.1646586345
307.2891566265

46.8812877264
153.0985915493

58.12
230.1

57.3947895792
279.619238477

56.9076305221
275.6827309237

Punto mximo Variable

Valor ptimo
codificado
no codificado

1
1.2
5.5

2
1.2
86

3
1
120

4
1
30

25
24
23
22
21
20
19
18
17
16
15
14
13
12
11
10
9
8
7
6
5
4
3
2
1

area /gramos muestra

8.764705882
8.389662028
9.402390438
6.923076923
8.059405941
8.821218075
7.6171875
7.193675889
8.359375
7.056530214
8.254901961
8.048289738
7.240704501
8.290766208
3.574144487
7.709163347
6.24015748
8.607350097
6.483300589
9.53815261
8.682634731
8.634538153
8.658536585
8.297029703
7.955911824

Punto mximo
Valor ptimo

Variable
codificado
no codificado

1
1.2
5.5

2
1.2
86

3
1
120

4
1
30

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25

(rea/g muestra)
628.256513
921.782178
943.272358
938.313253
960.958084
1012.89157
807.90927
978.317215
795.885827
797.051793
711.121673
913.202358
883.835616
813.118712
917.137255
895.282651
926.621094
957.608696
902.597656
868.192534
861.168317
915.283401
915.08
853.886719
964.110672

Punto mximVariable
Valor ptimocodificado
no codificado

1
1.2
5.5

2
1.2
86

3
1
120

4
1
30

2 (M22)
3 (M-8)

4 (M-25)

8.84818E-005 93.34669339
95.75150301 52.84569138
Valor ptimo
codificado

cido actico
(%v/v )

1.2

Metanol (%v/v )

1.2

Tiempo (min)

Temperatura
(C)

93.38677355 95.75150301

5 (M-13)

84.78957916 63.28657315

6 (M-9)

63.28657315 88.83767535

7 (M-17)

93.76753507 79.87975952

8 (M-18)
9 (M-2)
10 (M-24)
11 (M-7)
12 (M-19)
13 (M-21)
14 (M-16)
15 (M-10)
16 (M-20)
17 (M-3)
18 (M-11)
19 (M-15
20 (M-5)
21 (M-12)
22 (M-6)
23 (M-14)
24 (M-23)
25 (M-1)
27 (M-4)

Factor

91.76352705 85.49098196
89.51903808 84.78957916
86.63326653 82.20440882
52.84569138 88.0761523
87.6753507 88.23647295
86.11222445 93.76753507
88.23647295 91.76352705
88.83767535 87.6753507
93.74749499 93.74749499
88.45691383 86.11222445
79.87975952 8.84818E-005
88.0761523 92.54509018
90.68136273 86.63326653
85.49098196 93.38677355
93.34669339 88.45691383
82.20440882 90.68136273
92.54509018 88.15631263
87.35470942 87.35470942
88.15631263 89.51903808

Valor ptimo
decodificado

5.5
86
119.5
29.6

Experimento Y
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
1
2
3
4
5

Y^1,1
24.02
21.36
24.37
19.45
12.35
3.86
16.05
12.90
9.90
15.18
13.02
24.28
21.58
18.34
24.18
10.26
15.12
27.12
11.70
20.97
16.67
19.13
18.56
18.71
19.02

33.00
29.01
33.54
26.17
15.88
4.42
21.19
16.65
12.45
19.92
16.83
33.40
29.34
24.53
33.26
12.95
19.84
37.72
14.96
28.43
22.09
25.70
24.85
25.08
25.54

Factor
% cido
% metanol
tiempo
temperatura

Comentarios
Cuando se fijaron las variable

Valor ptimo codificado

Valor ptimo decodificado

1.2
1.2
1
1

5.5
86
120
30

do se fijaron las variables se asginaron los valores mximos. Si se asignaban valores medios o bajos, la respuest

ios o bajos, la respuesta segua siendo la misma

Cromatograma Flavonoles(355nm)
1 (M1-1uL)
Pico
tr
rea mAU*min Rel % area
14
14.09
0.528
4.03
15
14.217
6.96
53.12
16
14.367
0.583
4.45
17
14.91
0.541
4.13

M22

14
15
16
17

14.08
14.2
14.353
14.903

0.538
6.612
0.567
0.517

4.21
51.69
4.43
4.04

M8

14
15
16
17

14.07
14.187
14.343
14.897

0.501
8.002
0.682
0.611

3.12
49.72
4.24
3.8

M25

14
15
16
17

14.073
14.19
14.347
14.897

0.559
7.568
0.675
0.567

3.63
49.15
4.38
3.69

M13

14
15
16
17

14.07
14.19
14.343
14.893

0.535
6.241
0.556
0.483

4.1
47.85
4.26
3.7

M9

14
15
16
17

14.063
14.183
14.34
14.89

0.326
5.709
0.466
0.413

2.71
47.39
3.87
3.43

M17

14
15
16
17

14.067
14.183
14.343
14.893

0.52
7.715
0.769
0.611

3.5
51.87
5.17
4.11

M18

14
15
16
17

14.067
14.183
14.343
14.893

0.53
7.773
0.709
0.593

3.65
53.52
4.88
4.08

M2

14
15
16
17

14.07
14.183
14.343
14.893

0.504
7.211
0.632
0.539

3.44
49.2
4.31
3.67

M24

14
15
16
17

14.067
14.187
14.343
14.89

0.538
5.961
0.503
0.427

4.31
47.77
4.03
3.42

M7

14
15
16
17

14.063
14.183
14.34
14.89

0.385
6.292
0.585
0.483

3.16
51.76
4.82
3.97

M19

14
15
16
17

14.067
14.183
14.34
14.887

0.521
7.145
0.668
0.55

3.83
52.53
4.91
4.04

M21

14
15
16
17

14.063
14.18
14.337
14.883

0.528
5.624
0.487
0.415

4.41
46.92
4.07
3.46

M16

14
15
16
17

14.06
14.177
14.333
14.883

0.576
6.289
0.618
0.477

4.36
47.59
4.68
3.61

M10

14
15
16
17

14.06
14.173
14.333
14.877

0.515
7.578
0.612
0.567

3.55
52.28
4.22
3.91

M20

14
15
16
17

14.063
14.173
14.337
14.887

0.525
8.093
0.775
0.614

3.17
48.9
4.68
3.71

M3

14
15
16
17

14.06
14.173
14.337
14.883

0.521
3.85
7.275
53.77
0.669
4.94
0.535 6,874 3,96

M11

14
15
16
17

14.06
14.177
14.333
14.873

0.429
4.098
0.297
0.291

5.18
49.45
3.58
3.51

M15

14
15
16
17

14.06
14.173
14.337
14.887

0.547
6.757
0.641
0.506

4.32
53.34
5.06
3.99

M5

14
14.07
15
14.18
16
14.343
17 14,883 0,544

0.517
7.374
0.69
0.544

3.46
49.42
4.62
3.65

M12

14
15
16
17

14.06
14.173
14.333
14.877

0.527
4.07
6.905
53.33
0.634
4.9
0.5 6,357 3,86

M6

14
15
16
17

14.057
14.17
14.333
14.88

0.489
7.934
0.721
0.578

3.1
50.26
4.57
3.66

M14

14
15
16
17

14.06
14.173
14.333
14.87

0.509
5.186
0.465
0.366

5.11
52.15
4.68
3.68

M23

14
15
16
17

14.057
14.167
14.33
14.873

0.446
8.041
0.735
0.723

2.93
52.94
4.84
4.76

M1

14
15
16
17

14.053
14.167
14.33
14.87

0.476
7.273
0.685
0.517

3.24
49.52
4.66
3.52

M4

14
15
16
17

14.057
14.167
14.33
14.87

0.472
7.188
0.643
0.51

3.27
49.77
4.45
3.53

Masa (mg) A/g muestra

49.9 10.581162325
49.9 139.47895792
49.9 11.683366733
49.9 10.841683367
172.5851703
49.4
49.4
49.4
49.4

10.890688259
133.84615385
11.477732794
10.465587045
166.6801619

51.7
51.7
51.7
51.7

9.6905222437
154.77756286
13.191489362
11.818181818
189.4777563

51
51
51
51

10.960784314
148.39215686
13.235294118
11.117647059
183.7058824

51.1
51.1
51.1
51.1

10.469667319
122.13307241
10.880626223
9.4520547945
152.9354207

50.8
50.8
50.8
50.8

6.4173228346
112.38188976
9.1732283465
8.1299212598
136.1023622

51.2
10.15625
51.2 150.68359375
51.2 15.01953125
51.2 11.93359375
187.7929688
50.6
10.4743083
50.6 153.61660079
50.6 14.011857708
50.6 11.719367589

Cromatograma Sumatoria
1 172.58517034
2 175.96039604
3 182.92682927
4 176.96787149
5 182.13572854
6 195.22088353
7 152.1611002
8 189.47775629
9 136.1023622
10 184.70119522
11 97.243346008
12 168.29076621
13 152.93542074
14 131.30784708
15 165.70588235
16 155.16569201
17 187.79296875
18 189.82213439
19
173.515625
20 196.60117878
21 139.68316832
22 166.68016194
23 198.10756972
24 147.69383698
25 183.70588235

189.8221344
50.5
50.5
50.5
50.5

9.9801980198
142.79207921
12.514851485
10.673267327
175.960396

50.3 10.69582505
50.3 118.50894632
50.3
10
50.3 8.4890656064
147.693837
50.9 7.5638506876
50.9 123.61493124
50.9 11.493123772
50.9 9.489194499
152.1611002
51.2 10.17578125
51.2 139.55078125
51.2
13.046875
51.2
10.7421875
173.515625
50.5
50.5
50.5
50.5

10.455445545
111.36633663
9.6435643564
8.2178217822
139.6831683

51.3 11.228070175
51.3 122.59259259
51.3 12.046783626
51.3 9.298245614
155.165692
50.2 10.258964143
50.2 150.9561753
50.2 12.19123506
50.2 11.294820717
184.7011952
50.9
50.9
50.9
50.9

10.314341847
158.99803536
15.225933202
12.062868369

196.6011788
49.2 10.589430894
49.2 147.86585366
49.2 13.597560976
49.2 10.87398374
182.9268293
52.6
52.6
52.6
52.6

8.1558935361
77.908745247
5.6463878327
5.5323193916
97.24334601

51.0
51.0
51.0
51.0

10.725490196
132.49019608
12.568627451
9.9215686275
165.7058824

50.1 10.319361277
50.1 147.18562874
50.1 13.77245509
50.1 10.858283433
182.1357285
50.9
50.9
50.9
50.9

10.353634578
135.65815324
12.455795678
9.8231827112
168.2907662

49.8
49.8
49.8
49.8

9.8192771084
159.31726908
14.477911647
11.606425703
195.2208835

49.7
49.7
49.7
49.7

10.241448692
104.34607646
9.3561368209
7.3641851107
131.3078471

50.2
50.2
50.2
50.2

8.8844621514
160.17928287
14.641434263
14.402390438

198.1075697
49.9 9.5390781563
49.9 145.75150301
49.9 13.72745491
49.9 10.360721443
179.3787575
49.8 9.4779116466
49.8 144.3373494
49.8 12.911646586
49.8 10.240963855
176.9678715

Parmetro
Cod
Decod

1
1.2
5.5

2
1.2
86

3
1.0
119.5

4
1.0
29.6