Documentos de Académico
Documentos de Profesional
Documentos de Cultura
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Adsorption, penetration, uncoating nucleic acid and protein synthesis, intracellular trafficking,
assembly, maturation and release, viral-host interaction,
Module IX: Virus structures, animal and plant viruses: Virus structure and morphology,
viruses of veterinary importance and plant viruses: DNA and RNA viruses: Replication:
General strategies, replication of plus stranded RNA virus, negative strand: RNA viruses;
Replication of double stranded RNA virus, and retroviruses: Positive strand virus,.
Retroviruses; Replication of double stranded DNA viruses,;Viral diseases, pathogenesis,
Epidemiology, control, immunology, transformation, evolution, diagnostics, antiviral agents,
emerging diseases.
CDB 303: Cell and Developmental Biology (3 credits)
Module I: Biomembranes: Historical view; Molecular Architecture Models; Importance of
fluidity. Cholesterol, Domains and Microdomains, RAFTS; Intracellular protein traffic:
transport & sorting; Protein Sorting, Trafficking and Targeting the biology of protein
movement into membranes and organelles; Exosomes in biology; Post-translational
mechanism
Module II: Nucleus, chromosome and cell Cycle; Structure, nuclear pores, nucleosome
organization, nucleolus; Cell division (student to be pre-prepared), mechanism of
chromosome segregation; Cell Cycle: Cyclins and cyclin dependent kinases, Regulation of
cyclin - CDK activity retinoblastoma and E2F proteins.
Module III: Cytoskeleton: Microfilaments; Microtubules-structure & dynamics & mitosis; Cell
movements intracellular transport, kinesin and dyenin; Cilia & Flagella
Module IV: Cell Cell signaling & Signal transduction: Cell Signaling in animals: Signaling
from plasma membrane to nucleus including signaling via animal hormones;emphasis on
positioning of cellular machinery, components of various signalling pathways;Downstream
effects of signaling on cell adhesion, cell differentiation, invasion and programmed cell
death;Epithelial-Mesenchymal Transition in Biology: introduce students to issues of polarized
epithelial cell function in the context of normal physiology as well as alterations associated
with disease.
Module V: Plant hormone signaling: Introduction to Plant
Growth Regulators
(Hormones), Plant Hormone Discovery; A Systems Approach to Hormone Interactions; Major
Function of Auxins: Stem Elongation; Cytokinin Functions & Interactions, Brassinosteroids:
Similar to Auxins; Gibberellins: Stem Elongation, Fruit Growth, & Seed, Abscisic Acid,
Strigolactones; Plant Response: Events when Plants Produce Ethylene, Response to
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analysis); Repetitive and unique sequences; Satellite DNA; DNA melting and buoyant
density; Nucleosome; DNA methylation & Imprinting; Mendelian disorders; Mitochondrial
disorders
Module IV: Genome Maintenance (DNA Replication and Recombination);The Replication of
DNA;Replication initiation, elongation and termination in prokaryotes and eukaryotes;
Enzymes and accessory proteins; Fidelity; Replication of single stranded circular DNA;The
Mutability and Repair of DNA; Photoreactivation; Nucleotide excision repair; Mismatch repair;
SOS repair; Recombination: Homologous and non-homologous;;
Homologous
Recombination at the Molecular Level; Site Specific Recombination and Transposition of
DNA; Defects in Genome Maintenance
Module V: Gene Expression; Mechanisms of Transcription; Prokaryotic Transcription;
Transcription unit; Promoters- Constitutive and Inducible; Operators; Regulatory elements;
Initiation; Attenuation; Termination-Rho-dependent and independent; Anti-termination;
Transcriptional regulation-Positive and negative; Transcript processing; Processing of tRNA
and rRNA; Eukaryotic transcription and regulation; RNA polymerase structure and assembly;
RNA polymeraseI, II, III; Eukaryotic promoters and enhancers; General Transcription factors;
Transcriptional and post-transcriptional gene silencingRNA Splicing; Translation; Translation
machinery; Ribosomes; Composition and assembly; The Genetic Code
Module VI: Eukaryotic GeneRegulation;Transcriptional Regulation in Prokaryotes;
Transcriptional Regulation in Eukaryotes; Regulatory RNAs
Module VII: Epigentics; Control of transcription by DNA methylation, methyl DNA binding
proteins and recruitment of HDACs; Histone methyl transferases; Genomic Imprinting in
Mammals Mechanisms; RNAi Biochemical mechanisms
Module VIII: Molecular Evolution: Ecological Perspective; Population perspective
BBT 305: Basic Biotechniques (3 credits)
Module I: Basic principles of protein detection and quantification: Measurement of total
protein by colorimetry: Biuret, Lowry, bis-cinchinonic acid (BCA) and Bradford assays.
Commercial kits merits and demerits, Amino acid analysis, Spectroscopic methods,
Fluorescence emission methods; Basic principles and Methods of separation and purification
of proteins; Recovery of protein monitoring by SDS PAGE and Western blot. Fundamentals
of chromatography, diverse chromatographic techniques such as Gas chromatography,
HPLC, FPLC, and UPLC etc.
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Module VII: Research methods in cellomics: Cell culture techniques (Basics); Primary cells
and cell lines, Culture methods, Enzymatic , mechanical, Explant approaches, Cell culture
requirements, Media , FBS etc, Contamination and Cell culture lab requirements, GLP. Gene
delivery approaches: Transfection techniques, Electroporation, Lipid based transfection
methods, Dextran, CaCl2 methods. Microinjection, Viral gene delivery methods, Retroviral,
Adenoviral and Lentiviral transfection protocols.
Module VIII: Cell imaging: Basics of Microscopy: Bio-imaging; Introduction to important
concepts. Digital vs. analog images. Light microscopy; History, Latest developments and
trends. Basics of optics, resolution and Super resolution : Theories and techniques of
Fluorescent and confocal microscopy: Fluorescence microscopes and components, Live cell
fluorescence imaging, Multidimensional imaging: TEM, SEM, Scanning probe microscopy
(AFM) and Raman Confocal imaging: Principles, Components of Equipments, Image
formation principles, Sample preparation and image analysis, Advanced application in
imaging; Application and principles of FRET, FRAP, FLIM, Photo activation etc.
Cell analysis and cell separation techniques (FACS): Flow cytometry, Principles of diverse
flow cytometers and sorting approaches, Detection methods and compensation in flow
cytometry. Immunophenotyping; Sterile sorting of live cells; Commercial sorters and variants;.
Sample preparation for FACS
Module IX: Medical imaging approaches: Whole animal imaging approaches: Optical
imaging, CT, MRI, SPECT, MRI analysis of animal models of human diseases: Animal
models of angiogenesis and cancer: In vivo models, Developmental studies using animal
models, Angiogenesis studies in tissues, Principles and practices in angiogenesis research.
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