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Int. Res J Pharm. App Sci.

, 2013; 3(1): 90-95

ISSN: 2277-4149

International Research Journal of Pharmaceutical and Applied
Sciences (IRJPAS)
Available online at
Int. Res J Pharm. App Sci., 2013; 3(1): 90-95

Research Article
Ahmad Muhtadi, Rini Hendriani, Resmi Mustarichie*
Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia
(Received: 22 January, 2013; Accepted: 10 February, 2013; Published: 25 February, 2013)
Corresponding Author’s email:,
Abstract: Screening tests on Indonesian herbals as potential antidiabetic have been carried out. Herbals used were Cajanus
cajan, Gardenia augusta, Glycine max, Nephelium lappaceum, Fragaria ananassa, Leucaena leucocephala, Centella asiatica,
Carica papaya, Piper bettle var. Rubrum and Stachytarpheta mutabilis. The method used refers to the decrease in blood glucose
in male mice (Swiss webster). Phaleria macrocarpa used as a comparison with a dose of 14 mg/kg w/w. Measurement of blood
glucose levels were performed before additon of glucose and every 30 minutes for 120 minutes after a glucose solution was added
by using a glucometer. The test results showed that the test plant extracts have the ability to lower blood sugar levels. Largest
decrease in glucose levels occurred at minute 120. The greatest percentage decline occurred in the plant Centella asiatica, it could
even pass through the Phaleria macrocarpa decreased levels and the smallest found in Fragaria x ananassa. Comparison between
normal and negative controls gave a significance value.
Keywords: Indonesian herbals, antidiabetic, blood sugar.
Diabetes mellitus is an endocrine disease that affects many
people around the world. Diabetes for all age groups
worldwide was estimated at 2.8% in 2000 and 4.4% in 2030.
The total number of diabetics in the estimate rose from 171
million in 2000 to 366 million in 2030. Very high diabetes
suffered by men than women, but women have a higher risk
of contracting diabetes than men 1, 2.
Use of medication to control diabetes can use natural
medicines. The use of natural medicines is supported by
several favourable factors. First, the smaller side effects than
synthetic drugs because of the natural ingredients work
through several mechanisms which are activated by several
different chemical compounds so that the total had
significantly lower side effects. The second reason is more
secure because it works without any harmful properties of
the drug use could be as self-selected3.
Indonesia has medicinal plants as potential anti-diabetic)4,5,6.
Some of wich commonly used by the general public
includes hiris (Cajanus cajan (L) Millsp), kaca piring
(Gardenia augusta (L) Merr), strawberry (Fragraria x
ananassa Duch), rambutan (Nephelium lappaceum L), petai
Cina (Leucaena leucocephala L), soybean (Glycine max (L)
Merr, pegagan (Centella asiatica (L) Urban), papaya leaves
(Carica papaya L), sirih merah (Piper betle L. var.
Rubrum), keji beling (Strobilanthes crispus Bl.
Sirih merah (Piper betlle L. var. rubrum) are known to grow
in the cold air as red betel cannot thrive in hot regions. It is a
rare ornamental plant which empirically have different
efficacy as anti-cancer, anti-hypertensive, anti kidney
stones, anti-hepatitis, and haemorrhoid. This plant is also
known to have efficacy in the treatment of herbs that are
believed to have the effect of preventing premature
ejaculation, anticonvulsant, antiseptic, analgesic, antiAhmad Muhtadi et al, 2013

dandruff, anti-diabetic, liver protector, ant diarrheal,
maintaining immunity and relieving swelling. A red betel
leaf to overcome inflammation of the lungs, inflammation of
the throat, inflammation of the gums, inflammation of the
breast, bloody nose, and coughing up blood. Effect of active
substances contained red betel leaves also can stimulate the
central nervous and thinking. Known chemical content
based on the results of research using thin layer
chromatography of flavonoids, alkaloids, compounds called
polyphenols, tannins, saponins, and volatile oil7.
Soybeans (Glycine max (L) Merr grown in Indonesia is the
kind of yellow and black. Yellow soybeans are widely used
in industry tempe, tofu, and milk. While the black soy sauce
used in the manufacture. Soy contains many black
anthocyanins the pigment that has antioxidant activity8.
Genistein and daidzein isoflavones contained in soy is
known to have anti-cancer properties 9.
Strawberry (Fragaria x ananassa Duch.) is found in Europe
and Asia. Most of the crop is cultivated in subtropical
regions and in the tropics. In the tropics was planted on a
plateau. Parts of this plant are used for the treatment are the
leaves, roots and fruit ripening. Fruit, especially seeds and
antimutagenic, anti-carcinogenic role. Its roots can be used
as a diabetes drug. Its leaves can be used to compress the
outer wounds, inflamed digestive tract, respiratory tract
inflammation, diarrhea, kidney disorders such as kidney
stones and a diuretic, liver disease, jaundice, rheumatoid
arthritis, gout, fever medication, and tense nerves10.
Pegagan (Centella asiatica (L.) Urban), is a wild plant in
Indonesia and tropical climatic regions, in general from the
plains to a height of 2,500 meters above sea level. Centella
asiatica growing in the open or slightly shaded, on damp soil
and lush as in the moor, pasture, or in the field11. Centella
works to improve blood circulation, laxative urine (diuretic),


3(1): 90-95 increased neural memory. black hair. namely ethanol (70% & 96%). cancer sores. namely stirred bar. and scales. cholesterol. Test group 3: Strawberry (Fragraria x ananassa Duch) + PGA 2% + glucose 2. diabetes mellitus. papaya leaves (Carica papaya L ).Int. roots. ISSN: 2277-4149 Rubrum). difficult defecation15. vacuum pumps. knives. Part used: Leaves. rheumatism. Diseases that can be treated diabetes mellitus. Besides the leaves. stimulate growth of nails. Comparison group: Mahkota Dewa (Phaleria macrocarpa) + PGA 2% + 2. namely: 1. abdominal pain during menstruation. macerator. Keji beling (Strobilanthes crispus Bl).8 mg/kgw/w. Plant part used is the skin of the fruit. piles (hemorrhoids). analytical scales. Treatable disease: kidney stones. Tools The tools used in the extraction process. treat leprosy. gray hair. saponins. This study aims to screen all ten plants traditionally known as antidiabetic drugs by the method of reduction of blood sugar in mice. hypertension. measuring cups.2 Methods The method used to obtain the extra plants by performing the extraction stage. syringes. weight 20-30 grams. Ahmad Muhtadi et al.8mg/kgw/w 9. glucose. excessive menstruation. Hiris (Cajanus cajan L. cough with phlegm.8 mg/kg w/w 4. soybean (Glycine max L. roots and papaya latex also contains papayotin. Rind used to treat dysentery. laxative fart. antispasmodic. var. Test group 4: Rambutan (Nephelium lappaceum L.) Merr. saponins and tannins. kautsyuk. Petai Cina (Leucaena leucocephala Benth). diabetes drugs. urinary tract disorders. 2013 2. cure wounds and ulcers.). diarrhea. 2013. saponins. experimental animals used were white mice (Mus muculus) male.) The name rambutan is derived from the Indonesian word rambutan. gotu kola also works to increase the repair and strengthening skin cells. 2. and polyphenols while stem contains flavonoids. vaporizer bowls. Seeds lamtoro efficacious as anthelmintic. 6. Seeds used to treat diabetes (diabetes mellitus)16. Res J Pharm. for extraction. Besides lamtoro also be used as a contraceptive in men13. liver (jaundice).  Extraction Simplicia Macerated by EtOH 70 % & 96% Liquid extract Evaporated Viscous extract   Test of blood sugar levels drop Research methods to test the activity of insulin sensitivity by dividing the mice into 13 groups with each group number as many as five animals. Millsp)14 leaves contain flavonoids. stopwatch. bark. meaning "hairy": diseases that can be treated: Rind efficacious as fever. abdominal disorder. Bark used to treat cancer sores. and stimulants. myosin. Materials & Methods 2. 8. kaca piring (Gardenia augusta (L. and roots. can hit the snake and the black ant. pipette drops. 7. 5. strawberry (Fragraria x ananassa Duch). worms. While its roots are widely used as a laxative menstruation. karpain. leucorrhoea. to test blood sugar levels decrease in the glucose meter and glucose test strips GlukoDr  Blood Test Meter. Test group 5: Petai Cina (Leucaena leucocephala Benth) + PGA 2 + glucose 2. ulcers. sprue. restrainer. glass beaker. Test group 6: Keji beling (Strobilanthes crispus Bl) + PGA + 2% glucose + 2. saponins.) Merr. Kaca Piring (Gardenia augusta L. Diseases that can be treated tumors.8 mg/kgw/w8. age 3-4 months.1 Materials The materials used are simplicia of hiris (Cajanus cajan (L) Millsp). antitumor.8 mg/kg w/w 3. seeds. acne. improve digestion. leaves contain alkaloid. Sirih merah (Piper betle L.8 mg/kg w/w Test group 2: Kaca piring (Gardenia augusta (L. Papaya (Carica papaya L. lamtoro (Leucaena glauca Benth). Gardenia augusta and Gardenia jasminoides of research experts substances known to have oil evaporated compounds. malaria. Rambutan (Nephelium lappaceum L.) originally from Central America. Petai china. eczema.8 mg/kg 91 .) + PGA 2% + glucose 2. and the PGA. Nutritious seeds lower blood sugar (hypoglycemic). Leaves are used to treat diarrhea. Curable Disease: Jaundice (jaundice). bruises. In addition. and seeds. Keji Beling (Strobilanthes crispus Bl) leaves contain alkaloids. App Sci. Merr). rambutan (Nephelium lappaceum L. Normal Control Group: PGA 2% 2. leaves. to test decrease blood sugar levels the distilled water. flavonoids and tannins. fever. hypertension.. malnutrition. flavonoids and polyphenols17. Roots used to treat fever. Pegagan (Centella asiatica (L) Urban).) + PGA 2% + glucose 2. karposit and vitamins4.). hair and connective tissue12. diarrhea. Negative (-) Control Group: PGA 2% + glucose 2. rotary evaporator.Merr ) is an annual herbaceous tribe of coffee-copy or Rubiaceae. cuts.8 mg/kg w/w Test group 1: Hiris (Cajanus cajan (L) Millsp + PGA 2% + glucose 2. fever. protein. cough. dysentery.

Graph of the average blood glucose levels of mice on the impairment testing of blood sugar levels can be seen in Figure 1.8 mg/kg w/w ISSN: 2277-4149 Pegagan (Centella asiatica (L ) Urban).36 33. 3(1): 90-95 10. Rubrum) and papaya addressing significant results against a Normal control and Rambutan (Nephelium lappaceum) seeds. Pegagan (Centella asiatica (L) Urban). cajans (b).33 16. besides the soybean group. From below figures (see Fig. 30.76 21. x ananassa (d).33 16. Res J Pharm. 2013 How to calculate of blood sugar levels that make use of glucose tolerance is a pretty prevalent used today.2 47.) group. 60. The end mice cut. Simplisia a Weight (g) b c d e f g h i j 23. rubrum) + PGA 2% glucose + glucose 2.05. rubrum). The test group 10: Sirih merah (Piper betle L. seed Rambutan (Nephelium lappaceum).95 27. Ahmad Muhtadi et al. var. 12. augusta (c). Papaya Leaf (Carica papaya L).49 63. fruit Petai China (Leucaena leucocephala). then the blood dripped to the end of the strip and after 20 seconds the blood glucose level will appear on the monitor glucose meter. Soy bean (Glycine maxmenr). C. C. Newman-Keuls test performed to determine the decrease in blood sugar levels of the test plants in mice given the highest impact on blood sugar levels drop. To prove that the blood sugar levels will decline after addition of the 10 extracts.52 23.24 Rendemen (%) 23. L.95 27. Sirih merah (Piper betle L.8 mg/kg w/w Table 1.49 63.95 23. Test group 7: Soybean (Glycine max L. while the Kaca piring (Gardenia augusta (L. it can be seen significant results in the Hiris (Cajanus cajan (L) Millsp group againts the negative (-) control . blood sampling performed on all mice before administration of glucose and insulin (t = 0).8 mg/kg w/w Given the same treatment for all of these groups. kaca piring plates (Gardenia augusta). To Fig. From the observations decrease blood sugar levels in the blood of mice.05. crispus Blood glucose measurements performed using a glucose meter and glucose test strips. S.Int. 90 and 120) after being given glucose 14 mg / kg w/w. F. G. App Sci. P. G.36 33. Keji beling (Strobilanthes crispus Bl) of 100 g macerated with ethanol 70% and fruits Strawberry (Fragraria x ananassa) of 100 g macerated with 96% ethanol with stirring a few times that aims to allow the flow of fresh solvent repeatedly came across the surface simplicia. Test group 9: Papaya leaves (Carica papaya L) + PGA 2% + glucose 2. and Newman-Keuls test. 2. Rambutan (Nephelium lappaceum) seed. mar (h).95 23. we should know the difference of the blood sugar levels decrease between the treatment group and the control and the comparison groups. Another common way is to use alloxan induction. The test is performed when the ANOVA test results obtained from the data are significantly different or significantly. Sirih Merah (Piper betle L. N. Results and Discussion Simplicia Hiris deciduous plants (Cajanus cajan (L) Millsp). asiatica (g). For testing glucose levels elapsed areas typically use fasting blood sugar 2 hours.76 21. var. Mahkota Dewa (Phaleria macrocarpa).24 Note: (a). leucocephala (f). Petai Cina (Leucaena leucocephala) and negative (-) control yielded significant results only for the N (normal) control group. Analysis was conducted Variants (ANOVA) Statistical analysis using Student's t test on a real level of 0. 2013.. betle (j). Average blood glucose levels were analyzed statistically using Student's t to see the difference in blood sugar levels decrease compared with the comparator plant Phaleria macrocarpa. control (N) and Petai Cina (Leucaena leucocephala).20 18. Extraction rendemen results 13. Keji beling (Strobilanthes crispus Bl. var. 3. Test group 8: Pegagan (Centella asiatica (L) Urban) + PGA 2% + glucose 2. 92 .20 18. C. Merr) + PGA 2% + glucose 2.8 mg/kg w/w 11. obtained data on the number average reduction in blood sugar levels for 2 hours. lappaceum (e).) Merr. papaya (i). and every 30 minutes to 120 minutes (minutes 0. 5) along with calculation of Student's t test with a real level of 0.2 47.52 23. Fasted 2 hours.

App Sci. 2 The average percentage reduction in blood glucose levels (%) of mice in the 30th minutes 40 35 30 25 20 15 10 5 0 mahkota dewa pegagan Kacapiring Kejibeling Papaya Kedelai Lamtoro 60 Minute Rambutan Hiris Fig 3: The average percentage reduction in blood glucose levels (%) of mice in the 60th minutes Ahmad Muhtadi et al. Res J Pharm.. 2013.Int. 2013 93 . 3(1): 90-95 ISSN: 2277-4149 Average blood glucose level (%) 350 K(N) 300 K(-) 250 Mahkota dewa 200 Pegagan Kacapiring 150 Kejibeling 100 Papaya 50 Kedelai Lamtoro 0 0 30 60 90 120 Rambutan Hiris Minute Avberage percentage reduction in blood glucose level (%) Fig.1 The average blood glucose levels (%) of mice on activity assays medicinal plant extracts 50 mahkota dewa 40 pegagan 30 Kacapiring 20 Kejibeling 10 Papaya 0 Kedelai 30 Minute Lamtoro Rambutan Average percentage reduction in blood glucose level (%) Fig.

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Res J Pharm. 3(1): 90-95 ISSN: 2277-4149 96 ..Int. 2013. App Sci.