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PHR 4600 Tuesday Afternoon Lab Section

Rat Stomach Fundus (Smooth Muscle Stimulation)


Data Compiled In Class on 11/18/14
Experiment #1: Dose-response for ACh-mediated smoth muscle contraction (g)
Final [ACh] (M)
1.00E-08
3.00E-08
1.00E-07
3.00E-07
1.00E-06
3.00E-06
1.00E-05
3.00E-05
1.00E-04
3.00E-04
1.00E-03

Smooth Muscle Contraction (grams


Average
Std Dev
n
0
0.000
0
0
0
0
0.000
0
0
0
0
0.000
0
0
0
0.194 0.157124
0.022
0.23
0.33
0.050
0.41
0.64 0.366667 0.297377
0.44 0.327414
0.080
0.52
0.72
0.51 0.359305
0.250
0.36
0.92
1.050
0.78
1.25 1.026667 0.235867
1.350
1.83
1.97 1.716667 0.325167
2.460
2.47
2.98 2.636667 0.297377
4.560
4.7
4.2 4.486667 0.257941

3
3
3
3
3
3
3
3
3
3
3

Experiment #2: Dose-response for ACh-mediated smoth muscle contraction (g) pre
Final [ACh] (M)

Smooth Muscle Contraction (grams


Average
Std Dev
n
0
0
1.00E-08
0
0
0
0
0
3.00E-08
0
0
0
0
0
1.00E-07
0
0
0
0.12
3.00E-07
0.35
44 14.82333
25.268
0.95
0.88 0.088882
1.00E-06
0.78
0.91
0.95
3.00E-06
0.88
1.79 1.206667 0.506392
1.63
1.33 0.860174
1.00E-05
0.36
2
2.25
3.00E-05
0.78
2.62 1.883333 0.973259
3.45
1.00E-04
2.47
2.95 2.956667 0.490034
4.25
3.11 1.216059
3.00E-04
1.83
3.25
5.55
1.00E-03
3.09
4.3 4.313333 1.230054

3
3
3
3
3
3
3
3
3
3
3

Experiment #3: 3 x 10-6 M Pilocarpine


Final [Pilo] (M)
3 x 10 M Pilocarpine
-6

Smooth Muscle Contraction (grams)


3.86

5.730

Average

Std Dev

3.1 5.563333 1.111077

Experiment #4: Dose-response for ACh-mediated smoth muscle contraction (g) pretreated with 3 x 10 -6 M ph
Final [ACh] (M)

Smooth Muscle Contraction (grams


Average
Std Dev
n
1.00E-08
0.13
0.1
0.09 0.106667 0.020817
3.00E-08
0.29
0.36
0.56 0.403333 0.140119
0.47 0.202978
1.00E-07
0.25
0.51
0.65
0.69 0.134536
3.00E-07
0.8
0.54
0.73
1.37 0.334215
1.00E-06
1
1.65
1.46
1.9 0.455741
3.00E-06
1.57
2.42
1.71
1.00E-05
2.75
3.6
2 2.783333 0.800521
3.00E-05
3.83
4.41
2.01 3.416667 1.252251
1.00E-04
3.93
4.81
2.56 3.766667 1.133858
3.00E-04
4.34
5.13
3.44 4.303333 0.845596
5.05 0.677348
1.00E-03
4.51
5.81
4.83

3
3
3
3
3
3
3
3
3
3
3

Experiment #5: 3 x 10-6 M Pilocarpine pretreared with 3 x 10-6 M physostigmine


Final [Pilo] (M)

Smooth Muscle Contraction (grams


Average

3 x 10 M Pilocarpine
-6

2.1

1.2

0.9

1.4

Std Dev

0.6245

Experiment #6: Dose-response for ACh-mediated smoth muscle contraction (g) pretreated with 1 x 10 -7 M at
Final [ACh] (M)

Smooth Muscle Contraction (grams


Average
Std Dev
n
0
1.00E-08
0
0.000
0
0
0
3.00E-08
0
0.000
0.00
0
0
1.00E-07
0
0.000
0.00
0
0
3.00E-07
0
0.000
0.00
0
1.00E-06
0.05
0.000
0.00 0.016667 0.028868
3.00E-06
0.25
0.600
0.00 0.283333 0.301386
1.00E-05
0.75
0.500
0.00 0.416667 0.381881
1.35 1.261943
3.00E-05
1.55
2.500
0.00
1.00E-04
2.35
3.500
2.00 2.616667 0.78475
5.15 0.934077
3.00E-04
4.15
5.300
6.00
6.75 1.033199
1.00E-03
5.65
6.900
7.70

3
3
3
3
3
3
3
3
3
3
3

Experiment #7: 3 x 10-6 M Pilocarpine pretreared with 1 x 10-7 M atropine


Final [Pilo] (M)
3 x 10 M Pilocarpine
-6

Smooth Muscle Contraction (grams)


0

Average
0.1 0.033333

Std Dev
0.05

ntraction (g)

1.00E-08
3.00E-08
1.00E-07
3.00E-07
1.00E-06
3.00E-06
1.00E-05
3.00E-05
1.00E-04
3.00E-04
1.00E-03

Std Error
0
0
0
0.090716
0.171691
0.189033
0.207445
0.136178
0.187735
0.171691
0.148922

ntraction (g) pretreated with 1 x 10 -6 M Tubocurarine


Std Error
0
0
0
14.58848
0.051316
0.292366
0.496622
0.561911
0.282921
0.702092
0.710172

Std Error
4 0.555538
Pilocarpine

5.563333
3 x 10 M Pilocarpine
-6

0.555538

ted with 3 x 10 -6 M physostigmine


Std Error
0.012019
0.080898
0.117189
0.077675
0.192959
0.263122
0.462181
0.722988
0.654633
0.488205
0.391067

Response of Pilocarpine With Other C


Smooth Muscle Contraction (grams)

6
5
4
3
2
1
0

Chemical Experime

Std Error
0.360555

ted with 1 x 10 -7 M atropine


Std Error
0
0
0
0
0.016667
0.174005
0.220479
0.728583
0.453076
0.53929
0.596518

Std Error
4

0.025

Exp 1

Exp 4

Exp 6

0 0.106667
0
0 0.403333
0
0
0.47
0
0.194
0.69
0
0.366667
1.37 0.016667
0.44
1.9 0.283333
0.51 2.783333 0.416667
1.026667 3.416667
1.35
1.716667 3.766667 2.616667
2.636667 4.303333
5.15
4.486667
5.05
6.75

PilocarpinePilocarpine and atropine

1.4 0.033333

0.118492
0
0
0
0.090716
0.171691
0.189033
0.207445
0.136178
0.187735
0.171691
0.148922

0.314812
0.012019
0.080898
0.117189
0.077675
0.192959
0.263122
0.462181
0.722988
0.654633
0.488205
0.391067

0.248056
0
0
0
0
0.016667
0.174005
0.220479
0.728583
0.453076
0.53929
0.596518

0.360555

0.025

Maximum Acetylcholine Response

e With Other Chemicals


6
5
4
3
2
1
0

Chemical Experimented With

Smooth Muscle Contraction (grams)

Dose
Response Curve of Acetylcholine and Chemi
8
7
6
5
4
Smooth Muscle Concentration (grams)
3
2
1

Maximum Ac
Maximum
Tubucurarine
0

4.486667 4.313333
0.00E+00

2.00E-04

4.00E-04

6.00E-04

8.00E-04

Final Acetylcholine Concentration (M)

1.00E-03

1.20

ylcholine Response Against Maximum Tubucurarine

Muscle Contraction (grams)

4.55
4.5
4.45
4.4
4.35
4.3
4.25
4.2

Chemical Experimented With

ine and Chemicals

Acetylcholine
Acetylcholine and
Physostigmine
Acetylcholine and
Atropine

1.00E-03

1.20E-03

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