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INTENDED USE
Remel Mitis Salivarius Agar is a solid medium recommended for use in qualitative procedures for selective isolation of Streptococcus mitis,
Streptococcus salivarius, and enterococci from specimens of mixed bacterial flora and for differentiation of the viridans strains.
PRINCIPLE
Proteose peptone and tryptose supply amino acids and other nitrogenous compounds necessary for the growth of bacteria. Crystal violet,
potassium tellurite, and trypan blue are selective agents that inhibit most gram-negative bacilli and gram-positive bacteria except streptococci
and enterococci. Potassium tellurite is also a differential agent that is reduced by Enterococcus spp. to form black colonies. Sucrose and
dextrose are carbon energy sources. S. salivarius metabolizes sucrose and develops luxuriant colonies with a gum-drop appearance.
S. mitis and enterococci do not metabolize sucrose and, as a result, form smaller colonies on this medium. Trypan Blue is absorbed by
colonies of streptococci producing a blue color. Dipotassium phosphate is a buffer and agar is a solidifying agent.
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PROCEDURE
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Inoculate and streak the specimen as soon as possible after it is received in the laboratory.
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Include a nonselective agar plate (e.g., blood agar) to increase the chance of recovering organisms present in low numbers and to
provide an indication of other organisms present in the specimen.
INTERPRETATION
Typical colony morphology on Mitis Salivarius Agar:
Streptococcus salivarius - Large, pale-blue, mucoid colonies that are glistening (i.e., gum-drop) in appearance.
Streptococcus mitis Small, flat, hard colonies, blue in color with a domed center
Streptococcus mutans - Raised, convex, undulate, opaque, pale-blue colonies that are granular (i.e., frosted glass) in appearance.
Colonies may exhibit a glistening bubble on the surface due to excessive synthesis of glucan from sucrose.
Streptococcus sanguis - Raised, smooth, hard colonies embedded in agar
Enterococci Blue-black, shiny, and slightly raised colonies
Coliforms Colonies (inhibited) are brown in color
QUALITY CONTROL
All lot numbers of Mitis Salivarius Agar have been tested using the following quality control organisms and have been found to be acceptable.
Testing of control organisms should be performed in accordance with established laboratory quality control procedures. If aberrant quality
control results are noted, patient results should not be reported.
CONTROL
INCUBATION
RESULTS
LIMITATIONS
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BIBLIOGRAPHY
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Refer to the front of Remel Technical Manual of Microbiological Media for General Information regarding precautions, product storage and
deterioration, specimen collection, storage and transportation, materials required, quality control, and limitations.
ATCC is a registered trademark of American Type Culture Collection.
IFU 1615, Revised June 9, 2014
Printed in U.S.A.