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2015/07/14
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Atsushi Nakajima
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D Alert # 8
20150714-1

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2015/07/14

Wingnut (Juglandaceae) as a new generic host for Pityophthorus juglandis


(Coleoptera: Curculionidae) and the thousand cankers disease pathogen, Geosmithia
morbida (Ascomycota: Hypocreales)

Pityophthorus juglandis"thousand cankers disease"


Geosmithia morbida

Bibliographic information
SM Hishinuma, PL Dallara - The Canadian , 2015 - Cambridge Univ Press

URL
http://journals.cambridge.org/abstract_S0008347X15000371

Abstract/Summary
The walnut twig beetle (WTB), Pityophthorus juglandis Blackman (Coleoptera: Curculionidae), vectors a fungus, Geosmithia morbida
Kolak, Freeland, Utley, and Tisserat (Ascomycota: Hypocreales), which colonises and kills the phloem of walnut and butternut trees,
Juglans Linnaeus (Juglandaceae). Over the past two decades, this condition, known as thousand cankers disease (TCD), has led to the
widespread mortality of Juglans species in the United States of America. Recently the beetle and pathogen were discovered on several
Juglans species in northern Italy. Little is known about the extra-generic extent of host acceptability and suitability for the WTB. We report
the occurrence of both the WTB and G. morbida in three species of wingnut, Pterocarya fraxinifolia Spach, Pterocarya rhoifolia Siebold and
Zuccarini, and Pterocarya stenoptera de Candolle (Juglandaceae) growing in the United States Department of Agriculture-Agricultural
Research Service, National Clonal Germplasm Repository collection in northern California (NCGR) and in the Los Angeles County Arboretum
and Botanic Garden in southern California, United States of America. In two instances (once in P. stenoptera and once in P. fraxinifolia)
teneral (i.e., brood) adult WTB emerged and were collected more than four months after infested branch sections had been collected in the
field. Kochs postulates were satisfied with an isolate of G. morbida from P. stenoptera, confirming this fungus as the causal agent of TCD in
this host. A survey of the 37 Pterocarya Kunth accessions at the NCGR revealed that 46% of the trees had WTB attacks and/or symptoms of
G. morbida infection. The occurrence of other subcortical Coleoptera associated with Pterocarya and the first occurrence of the polyphagous
shot hole borer, a species near Euwallacea fornicatus Eichhoff (Coleoptera: Curculionidae), in Juglans are also documented.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Geosmithia,,,Geosmithia
morbida,,,Ascomycota

/Italy, /California,
/Juglans, /Juglandaceae,
/Curculionidae

Hypocreales,,

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D Alert # 8
20150714-2

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2015/07/14

A new species and a new combination of Terriera based on morphological and


molecular data

Terriera

Bibliographic information
TT Zhang, X Tong, YR Lin, CL Hou - Mycological Progress, 2015 - Springer

URL
http://link.springer.com/article/10.1007/s11557-015-1078-3

Abstract/Summary
A new Terriera species, T. elliptica on twigs of Rhododendron sp., was reported from Yunnan Province, China. T. elliptica is similar to
Lophodermium camelliicola in morphology and affiliation, but differs in having elliptic, non-curved ascomata and larger asci. In addition, ITS
rDNA sequences of the two species differ significantly. Based on morphological and molecular analyses, L. camelliicola was transferred to
Terriera.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Terriera,,,,
Lophodermium,,,,

/Rhododendron, /China,

Lophodermium camelliicola,,,,

/new species, /new

combination, /Yunnan

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D Alert # 8
20150714-4

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2015/07/14

A new Xylaria species from Iran

Bibliographic information
SA Hashemi, R Zare, SA Khodaparast - Mycologia , 2015 - mi.iranjournals.ir

URL
http://mi.iranjournals.ir/article_13603_0.html

Abstract/Summary
Three species of the genus Xylaria (Xylariaceae) are presented based on collections from Guilan Province, Iran. Both morphological and
molecular characteristics were used in order to identify these species. Xylaria longissima sp. nov. is described and illustrated as a new
Xylaria species and X. cf. striata and X. xylarioides are reported as new records from Iranian mycobiota. Xylaria longissima and X.
xylarioides were collected from wood of unknown dicotyledonous plants and X. cf. striata was collected from soil. Differences between these
species and other closely related taxa are discussed. The result of this study indicates that sequences of internal transcribed spacer (ITS)
region have sufficient resolution to distinguish major species groups.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Xylaria,,,,
Xylariaceae,,,

/Iran, /Xylaria,
/Xylariaceae

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D Alert # 8
20150714-5

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2015/07/14

Axenic Cultivation of Mycelium of the Lichenized Fungus, Lobaria pulmonaria


(Peltigerales, Ascomycota)

Lobaria pulmonaria

Bibliographic information
C Cornejo, C Scheidegger, R Honegger - bio-protocol.org

URL
http://www.bio-protocol.org/pdf/20150707044554237/1513 Axenic Cultivation of Mycelium of the Lichenized Fungus, Lobaria pulmonaria
(Peltigerales, Ascomycota).pdf

Abstract/Summary
[Abstract] Lichens are symbiotic organisms consisting of a fungal partner (the mycobiont) and one or more algal or cyanobacterial partners
(the photobiont); moreover lichen thalli comprise a plethora of epi- and endobiotic bacteria and non-lichenized fungi. Genetic markers are
the most promising tools for the study of fungal diversity. However, applying genetic methods to intimately admixed symbiotic organisms
typically requires the development of species-specific genetic markers, since DNA extraction from environmental specimens implicates the
acquirement of total DNA of all symbionts and their cohabitants. While the cultivation of the alga is straight forward, the axenic cultivation of
lichen-forming fungi is more difficult due to their very slow growth, as compared with the majority of non-lichenized taxa, and the presence
of saprophytic, endophytic and parasitic fungi within the lichen thallus. Moreover, lichen-forming fungi (predominantly ascomycetes, few
basidiomycetes) are oligotrophic organisms and thus adapted to nutrient poor conditions; in axenic culture on nutrient-rich media, as
normally used for mass production of fast-growing saprophytic fungi, they often autointoxicate. Most lichen-forming fungi are not obligately
biotrophic and thus can be cultured in the non-symbiotic state.

Here, we present a protocol for the isolation of the lichen-forming

ascomycete Lobaria pulmonaria into axenic culture and for mycelial mass culture as a source of pure fungal DNA. We describe the initiation
of axenic cultures on agar plates from germinating ascospores and explain the optimization of the in vitro growth in liquid medium. By
grinding the few dense, only centrifugally growing fungal colonies with a homogenizer we obtain lots of smaller, well growing colonies and
thus higher amounts of mycelium for DNA or RNA isolation (Honegger and Bartnicki-Garcia, 1991).

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

MyceliumFungus,,,,
Lobaria,,,,

DNA/DNA extraction,

Lobaria pulmonaria,,,

/Lobaria, /lichen

,Peltigerales,,
Ascomycota

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D Alert # 8
20150714-6

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2015/07/14

Cantharellus cyphelloides (Cantharellales), a new and unusual species from a


Japanese evergreen broad-leaved forest

Cantharellus cyphelloides

Bibliographic information
H Suhara, S Kurogi - Mycological Progress, 2015 - Springer

URL
http://link.springer.com/article/10.1007/s11557-015-1079-2

Abstract/Summary
We describe a new species, Cantharellus cyphelloides (Cantharellaceae, Cantharellales, Agaricomycetes), from a Japanese evergreen broadleaved forest based on morphological and molecular data. The new species differs from other Cantharellus species by its cyphelloid
basidiomata with the hymenophore produced on the adaxial surface (inner side of the cup), rather than the abaxial surface. Molecular
phylogenetic reconstruction, based on partial nLSU rDNA sequence data, and microscopic features place the new species in Cantharellus
subgenus Cinnabarinus. An emended description of subgenus Cinnabarinus is provided.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Cantharellus,,,Cantharellus
cyphelloides,,,Cantharellales

/new species, /forest

,Cantharellaceae,,
Agaricomycetes

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D Alert # 8
20150714-7

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2015/07/14

Chaetomium-like fungi causing opportunistic infections in humans: a possible role for


extremotolerance

Chaetomium

Bibliographic information
SA Ahmed, Z Khan, X Wang, TAA Moussa - Fungal Diversity, 2015 - Springer

URL
http://link.springer.com/article/10.1007/s13225-015-0338-5

Abstract/Summary
Members of the family Chaetomiaceae are ubiquitous ascosporulating fungi commonly, which reside in soil enriched with manure or
cellulosic materials. Their role as human pathogens is largely ignored. However, the ability of some species to grow at high temperature
enables them to play an important role as opportunistic pathogens. The family contains several genera and species that have never been
reported to cause human infection. Hereby, three new species are described; two belong to the genus Subramaniula and one represents a
Chaetomium species. Subramaniula asteroides was isolated from various sources including eye and skin infections as well as from the
natural environment, and S. obscura was isolated from a toe infection. Chaetomium anamorphosum was isolated from a kidney transplant
patient suffering from fungal peritonitis. All species described were previously misidentified as Papulaspora spp. due to the formation of
cellular clumps or bulbil-like structures, which are characteristic of Papulaspora. The isolates failed to form sexual fruit bodies and
ascospores remained absent, which is an unusual feature for the generally ascosporulating genera Chaetomium and Subramaniula; minute
conidia from phialides were sometimes observed.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Chaetomiaceae,,,
Subramaniula,,,,

/new species, /temperature

Chaetomium,,,,
Papulaspora,

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D Alert # 8
20150714-8

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2015/07/14

Effects of Minor Elements on Cercospora kikuchii, Cercospora Leaf Blight and Rust on
Soybeans

CercosporaCercospora kikuchii

Bibliographic information
BM Ward - 2015 - etd.lsu.edu

URL
http://etd.lsu.edu/docs/available/etd-07022015-144703/

Abstract/Summary
Soybean (Glycine max) is one of the most widely grown crops in the world. Many pathogens attack soybeans, but of particular importance
to tropical and subtropical areas such as Louisiana is Cercospora leaf blight (CLB). This disease is caused by the fungus Cercospora kikuchii
and favored by high temperatures. This fungus utilizes a toxin, cercosporin, as its primary pathogenicity factor. Soybean rust (SBR), caused
by Phakopsora pachyrhizi, is another common disease that occurs in Louisiana under cooler environmental conditions of spring and fall. Both
diseases occur regularly in Louisiana and may result in severe yield losses. Cercospora kikuchii has recently developed fungicide resistance,
and there are few if any resistant soybean cultivars. For these reasons, a search for new management strategies is underway. This study
analyzed the efficacy of using plant-essential micronutrients applied foliarly to suppress CLB and SBR. This was tested in field experiments
on these diseases as they occurred, as well as on isolates of C. kikuchii on amended agar media; both in randomized complete block design.
Disease severity and yield were measured in field experiments, while fungal growth and toxin production were measured in vitro for C.
kikuchii. Results showed that copper (150 300 g ha-1) and high rates of aluminum (160 190 g ha-1) and iron (200 400 g ha-1)
applied foliarly to soybeans were effective in suppressing CLB severity. Iron; in the forms of iron nitrate and iron sulfate, as well as low rates
of aluminum (20 100 g ha-1) were effective in reducing SBR severity. Agar media amended with iron (> 250 mg L-1), manganese (> 250
mg L-1) and zinc (> 125 mg L-1) inhibited fungal growth at high physiological concentrations found in soybean leaf tissue. Additions of
aluminum (up to 150 mg L-1) and copper (up to 35 mg L-1) resulted in increased fungal growth. Aluminum, manganese, and zinc

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Cercospora,,,,
Cercospora kikuchii,,,

/Glycine, /Louisiana

,Phakopsora,,,
Phakopsora pachyrhizi,,,
Cercospora sojina,,,,

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D Alert # 8
20150714-9

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2015/07/14

Especies clnicas comunes del gnero Scopulariopsis Bainier y taxas relacionados

Scopulariopsis

Bibliographic information
E Piontelli - Boletn Micolgico, 2015 - portals3.uv.cl

URL
http://portals3.uv.cl/index.php/Bolmicol/article/view/26

Abstract/Summary
Resumen Los integrantes del gnero Microascus y su anamorfo Scopulariopsis, incluyen especies fngicas comunes del suelo, excrementos,
ambientes internos y algunas reconocidas como oportunistas en los animales, incluyendo al hombre. La sola morfologa del gnero no
siempre permite un buen diagnstico a pesar de su utilidad cuando se presentan los anamorfos o teleomorfos o ambos. Sin embargo, la
biologa molecular ha permitido el anlisis de muchos aislados clnicos, contribuyendo con nuevos aportes y nuevas combinaciones
taxonmicas en la literatura actual. Este trabajo, reune lo ms actual sobre estos gneros comunes en salud pblica, aportando claves
morfofisiolgicas tiles para su reconocimiento primario en los laboratorios clnicos de baja complejidad.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Scopulariopsis,,,,
Microascus,,,,

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D Alert # 8
20150714-10

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2015/07/14

Evolutionary study on mycoheterotrophy of Cephalanthera in Japan

Bibliographic information
- 2015 - ir.library.tohoku.ac.jp

URL
http://ir.library.tohoku.ac.jp/re/handle/10097/60336

Abstract/Summary

Comment (brief comment in Japanese)

Keywords(auto-indexed)
/Japan

Related taxa
(extracted from title and abstract/summary)

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D Alert # 8
20150714-11

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2015/07/14

Existence of Muscodor vitigenus, M. equiseti and M. heveae sp. nov. in leaves of the
rubber tree (Hevea brasiliensis Mll. Arg.), and their biocontrol potential

Muscodor vitigenusM. equisetiM. heveae

Bibliographic information
S Siri-udom, N Suwannarach, S Lumyong - Annals of Microbiology, 2015 - Springer

URL
http://link.springer.com/article/10.1007/s13213-015-1126-x

Abstract/Summary
We isolated volatile metabolite-producing endophytic fungi from the leaves of the rubber tree (Hevea brasiliensis Mll.Arg.) and studied their
antimicrobial competence. A total of three isolates was obtained, and their phenotypic and phylogenetic relationship with the genus
Muscodor in the family Xylariaceae was studied. All isolates could produce volatile metabolites with apparent antimicrobial activity against
diverse test microbes (bacteria, yeast and filamentous fungi). An isolate, RTM5IV3, with <86 % similarity with the partial ITS-5.8S rDNA
gene as compared to other species of the genus Muscodor, was proposed as a novel species with the name Muscodor heveae sp. nov. Its
bioactive volatile metabolites included 3-methylbutan-1-ol as a major component, followed by 3-methylbutyl acetate and azulene
derivatives. The volatile organic compounds (VOCs) produced by the Muscodor isolates have the potential for biological control of bacteria,
yeast and filamentous fungi. Furthermore, the VOCs of M. heveae were active against the pathogenic fungi Phellinus noxius and Rigidoporus
microporus that cause root disease in the rubber tree. We concluded that rubber trees could be an alternative source for discovery of fungi
that produce volatile metabolites, and the genus Muscodor could be found abundantly in this habitat.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Muscodor,Muscodor vitigenus,
Xylariaceae,,,

/Xylariaceae,

Phellinus,,,

/Hevea, /pathogenic fungi,

Phellinus noxius,,,

/yeast

Rigidoporus,,,
Rigidoporus microporus,,,

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D Alert # 8
20150714-13

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2015/07/14

PROXIMATE ANALYSIS AND CHEMICAL COMPOSITION OF CORTINARIUS SPECIES

Bibliographic information
PT Olagbemide, TA Ogunnusi - European Journal of Advanced , 2015 - idpublications.org

URL
http://www.idpublications.org/wp-content/uploads/2015/07/PROXIMATE-ANALYSIS-AND-CHEMICAL-COMPOSITION-OF-CORTINARIUSSPECIES.pdf

Abstract/Summary
Proximate study was conducted on a Cortinarius sp found on farmlands in Akinyele Local Government Area of Oyo state of Nigeria to
ascertain its nutritional composition. The overall nutritional potential of the Cortinarius species was quite good and it shows that the species
of mushroom is highly nutritive with crude protein 19.47%, carbohydrates, 48.60% and crude fibre 6.80%. It was however, observed the
contents of the following were relatively low; zinc was 0.08mg/100g, riboflavin 0.08mg/100g and thiamine 0.07mg/100g. Potassium was
highest and was 221.67mg/100g followed by calcium 183.33mg/100g. Phytochemical screening revealed the presence of varying quantities
of alkaloids, saponins, tannins, oxalates, cyanogenic glycosides and phytates. These bioactive compounds may make it useful for therapeutic
uses. This study shows that this mushroom has a great potential in complementing protein and minerals deficiencies prevalent in the
developing countries.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/screening, /Nigeria

Cortinarius,,,,

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D Alert # 8
20150714-14

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2015/07/14

Spelling out Jaapia species

Jaapia

Bibliographic information
MT Telleria, M Dueas, I Melo, I Salcedo - Mycological Progress, 2015 - Springer

URL
http://link.springer.com/article/10.1007/s11557-015-1081-8

Abstract/Summary
Jaapia is a wood-saprobic genus of corticioid fungi for which two species have been recognized: J. argillacea Bres. and J. ochroleuca (Bres.)
Nannf. & J. Erikss. Whereas the first one is easily recognized by its characteristic spores, the descriptions of the second indicated variable
spores, which once led us to believe that J. ochroleuca could be a species complex rather than a single species. Eleven new ITS nrDNA
sequences of J. ochroleuca were aligned with two obtained from GenBank and four of J. argillacea. The molecular results, parsimony
analysis and KP2 distances clearly delimitate one highly supported Jaapia clade, with two subclades that correspond to the two described
species. Morphological studies, including the holotype and isotype of J. ochroleuca, show significant differences between the clades
concerning the size and shape of spores. The present study corroborates two species in this genus and also confirms that J. ochroleuca is a
well-defined species in which spores show great morphological variability. Based on the Jaapia species hypothesis, the J. ochroleuca
reference sequence has been selected. A comprehensive key to two Jaapia species is also provided.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/corticioid fungi,
/species complex

Jaapia,,

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D Alert # 8
20150714-15

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2015/07/14

Strobilomyces pteroreticulosporus (Boletales), a new species of the S. strobilaceus


complex from the Republic of Korea and remarks on the variability of S. confusus

Strobilomyces strobilaceusS. pteroreticulosporusS. confusus

Bibliographic information
V Antonin, A Vizzini, E Ercole, M Leonardi - Phytotaxa, 2015 - biotaxa.org

URL
http://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.219.1.6

Abstract/Summary
Abstract A new species, Strobilomyces pteroreticulosporus, is described based on two recent collections from the Republic of Korea. This
new taxon is well characterized by morphological characters, and proved using rpb1 and ITS2 sequences. The variability of size and
basidiospore ornamentation of the common species S. confusus is also discussed.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Strobilomyces,,,,Boletales
,,

/new species

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D Alert # 8
20150714-16

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2015/07/14

Taxonomic notes on the Acanthopsis disperma-hoffmannseggiana complex


(Acanthaceae, tribe Acantheae), with an interim key to members of the genus

Acanthopsis disperma-hoffmannseggiana

Bibliographic information
HM Steyn, AE van Wyk - Phytotaxa, 2015 - mapress.com

URL
http://www.mapress.com/phytotaxa/content/2015/f/pt00219p026.pdf

Abstract/Summary
Acanthopsis is a taxonomically poorly understood genus confined to arid parts of southern Africa. This contribution is the first detailed
assessment of the diversity within the Acanthopsis disperma-hoffmannseggiana complex, the latter of which comprises the bulk of the
specimens of the genus represented in herbaria. Species concepts are resolved and the misap-plication of names is noted. In addition to the
recognition of A. disperma and A. hoffmannseggiana, five new species of Acanthopsis formerly treated under these two names, are
described, namely A. tuba and A. ludoviciana (both confined to the Richtersveld, Northern Cape, South Africa), A. adamanticola (endemic to
southern Namibia), A. villosa (endemic to Bush-manland, Northern Cape) and A. dispermoides (endemic to the Western Cape, South
Africa). The names A. disperma and A. hoffmannseggiana are lectotypified here. An identification key to the members of the Acanthopsis
disperma-hoffmannseg-giana complex is provided, as well as an interim key to the major artificial groupings in the genus, the taxonomy of
some which still needs to be resolved.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Western,
/Acanthaceae, /Namibia,
/South Africa, /new species,
/identification key, /Western
Cape

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20150714-17

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2015/07/14

Taxonomic re-examination of several Japanese Stemphylium strains based on


morphological and molecular phylogenetic analyses

Stemphylium

Bibliographic information
D Kurose, T Misawa, T Suzui, K Ichikawa - Journal of General Plant , 2015 - Springer

URL
http://link.springer.com/article/10.1007/s10327-015-0607-x

Abstract/Summary
Most of the many Stemphylium species on record as plant pathogens in Japan have been identified by morphology. Using molecular
phylogenetic analysis of four loci (rDNA-ITS, EF-1, GPD, and vmaA-vpsA) combined, we re-examined the taxonomy of 31 Stemphylium
strains that had been identified morphologically before or after their deposit in the NIAS Genebank, Japan. In the present study, these
Japanese strains were separated into three groups (Groups A, C, and E). Sixteen Japanese strains formed a new subgroup (designated
Subgroup C2) that contained no type or ex-type strains of existing species. Four of the strains had been identified correctly, whereas the
other 27 strains appear to have been misidentified based on our phylogenetic analysis. We examined the conidial morphology of four
representative misidentified strains selected from each clade. While the morphological characteristics of a strain isolated from Welsh onion
resembles those of the S. vesicarium type, this strain belongs to Subgroup C2 rather than Subgroup C1 and, therefore, could not be
identified at the species level. Conversely, three pathogenic strains isolated from aster, asparagus, and Chinese chive were re-identified
based on our combined morphological and phylogenetic data. We propose the scientific names of the aster leaf spot pathogen as S.
lycopersici, and the asparagus Stemphylium leaf spot and Chinese chive brown leaf blight pathogens as S. herbarum.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Stemphylium,,,,

/Chinese, /molecular
phylogenetic analysis, /Japan

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D Alert # 8
20150714-18

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2015/07/14

Taxonomy, Phylogeny, Cultivation and Biological Activities of a Lentinus species from


Andaman & Nicobar Islands (India).

Bibliographic information
VP Sharma, S Kamal, RC Upadhyay - Emirates Journal of , 2015 - search.ebscohost.com

URL
http://www.scopemed.org/?mno=186502

Abstract/Summary
Abstract: Fruit bodies of Lentinus were collected from subtropical climate of Andaman &amp; Nicobar Islands (India). Pure culture was
prepared by tissue culture on Malt-extract-agar (MEA) medium at 252C. The specimen was studied for its identification, taxonomy and
phylogeny and identified as Lentinus sajor-caju by morphological and microscopical studies. The identification was confirmed through ITS
5.8S rDNA sequencing and sequence analysis. Its cultivation was done on saw dust and wheat bran. The fruit bodies were obtained at a
temperature of 282C and 80-85% RH. The cultivated Lentinus sajor-caju fruit bodies were analysed for nutritional and biological
properties and observed that the cultivated mushroom has good nutritional properties and antioxidant, reducing and DPPH free radical
scavenging properties are comparable to commercially cultivated Lentinula edodes strains. Copyright of Emirates Journal of Food &
Agriculture (EJFA) is the property of United Arab Emirates University, Faculty of Food & Agriculture and its content may not be copied or
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Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Lentinus,,,Lentinus
sajor-caju,,,Lentinula

/India, /temperature,

,,,,Lentinula edodes

/sequencing

,,,,

Daikinrin Daily Document Alert

D Alert # 8
20150714-19

- the latest mycological papers from Google Scholar


Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

Search Date

2015/07/14

Transcriptional Responses Associated with Virulence and Defence in the Interaction


between Heterobasidion annosum ss and Norway Spruce

Heterobasidion annosum

Bibliographic information
K Lundn, M Danielsson, MB Durling, K Ihrmark - PloS one, 2015 - dx.plos.org

URL
http://dx.plos.org/10.1371/journal.pone.0131182

Abstract/Summary
Abstract Heterobasidion annosum sensu lato is a serious pathogen causing root and stem rot to conifers in the northern hemisphere and
rendering the timber defective for sawing and pulping. In this study we applied next-generation sequencing to i) identify transcriptional
responses unique to Heterobasidion-inoculated Norway spruce and ii) investigate the H. annosum transcripts to identify putative virulence
factors. To address these objectives we wounded or inoculated 30-year-old Norway spruce clones with H. annosum and 454-sequenced the
transcriptome of the interaction at 0, 5 and 15 days post inoculation. The 491860 high-quality reads were de novo assembled and the
relative expression was analysed. Overall, very few H. annosum transcripts were represented in our dataset. Three delta-12 fatty acid
desaturase transcripts and one Clavaminate synthase-like transcript, both associated with virulence in other pathosystems, were found
among the significantly induced transcripts. The analysis of the Norway spruce transcriptional responses produced a handful of differentially
expressed transcripts. Most of these transcripts originated from genes known to respond to H. annosum. However, three genes that had not
previously been reported to respond to H. annosum showed specific induction to inoculation: an oxophytodienoic acidreductase (OPR), a
betaglucosidase and a germin-like protein (GLP2) gene. Even in a small data set like ours, five novel highly expressed Norway spruce
transcripts without significant alignment to any previously annotated protein in Genbank but present in the P. abies (v1.0) gene catalogue
were identified. Their expression pattern suggests a role in defence. Therefore a more complete survey of the transcriptional responses in
the interactions between Norway spruce and its major pathogen H. annosum would probably provide a better understanding of

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Heterobasidion,,,
Heterobasidion annosum,,,

/Norway, /stem rot,


/virulence, /fatty acid, /stem
rot, /sequencing

Daikinrin Daily Document Alert

D Alert # 8
20150714-20

- the latest mycological papers from Google Scholar


Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

Search Date

2015/07/14

Two new species and a new record of Lepiota (Basidiomycota, Agaricales) from the
Dominican Republic

Bibliographic information
A Justo, C Angelini, A Bizzi - Mycological Progress, 2015 - Springer

URL
http://link.springer.com/article/10.1007/s11557-015-1080-9

Abstract/Summary
Two new species of Lepiota (L. squamulodiffracta and L. sosuensis) and a new record (L. subgranulosa) are described based on collections
made in the Dominican Republic. The phylogenetic position of the species based on nrITS data is analyzed and discussed. L.
squamulodiffracta belongs in section Lepiota, and it is characterized by the velar patches on pileus split up into numerous minute
squamules, striate-sulcate pileus up to the center, and penguin-shaped spores. L. sosuensis has overall white basidiocarps, dextrinoid and
metachromatic spores, trichodermial pileus covering, and rare clamp-connections. L. subgranulosa stands out morphologically by the
spurred spores with one or several protuberances that give the spores an irregular outline.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Lepiota,,,,
BasidiomycotaAgaricales,,

/Dominican Republic,
/section, /new record,
/new species

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