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40 sq. m.
10 sq. m.
10 sq. m.
10 sq. m.
10 sq. m.
10 sq. m.
14 sq. m.
Interior passages
TOTAL
Sample receipt room
GRAND TOTAL
16 sq. m.
120 sq. m. (approx)
10 sq. m.
130 sq. m.
Set up partitions as in Fig 5, using aluminium and glass panelling. Put aluminium
or cement brick up to 1.2 meters from the floor and then glass up to the ceiling. At
ceiling level all the panels should fit tightly and be sealed with appropriate material
to prevent air leaks that may lead to contaminations.
All doors to be of aluminium /glass, opening outward. The door marked A should
have a width of 150 cm and the doors marked B a width of 130 cm to allow large
equipment to be carried in. Other doors could be of standard size.
Fix the central workbench in the main laboratory as indicated in the layout
diagram. (Fig. 5). There should be cupboards and drawers under the benches.
Construct workbenches along the wall in the glassware washing room, culture
maintenance room, media preparation rooms and in the main laboratory. These
should be 90 cm (height) x 90 cm (width) and suit the length to the walls as in Fig.
5. They may be of concrete or wood with push-in cupboards and drawers
underneath.
All bench and table tops in the testing laboratory, culture maintenance room, media
preparation room and washing room need to be laid with granite, formica or a
similar non-porous material.
The testing laboratory, the media preparation room and the culture maintenance
room need to be air-conditioned to maintain a continuous temperature of 25 oC and
RH (55 + 5)%.
In locations where an uninterrupted power supply is not available day and night, a
backup generator with automatic switch gear needs to be used.
The floor of the testing laboratory, the media preparation room and culture
maintenance room should be done with epoxy resin. In all other areas, use smooth
floor tiles, preferably white and of dimension 30cm x 30 cm.
The locations for electricity outlets for 15 amps and 3-phase supply are indicated in
Fig 6. Provide adequate lighting, using fluorescent or low energy bulbs to provide
light intensity of 750 lux. The lights should be fixed at ceiling level and not
hanging (to avoid collection and release of dust).
Locations of sinks are given in Fig 5. Stainless steel sinks are preferred. In the
washing room a sink with a draining board will be more useful. It is preferable to
fix two-way or three-way laboratory type taps for the sinks.
The water delivered to the laboratories should be at a minimum pressure of 2.0 bar
(Where the pressure of the public supply is low and not continuous, install an
overhead tank at 11metre height (equivalent of approximately the 4th floor of a
building).
The gas lines should carry two-way or four-way taps. The supply tank should be
located outside the laboratory with suitable safety valves and protection from
mishandling.
All water exhaust down pipes from the sinks should be of 50 mm diameter leading
down to 100 mm diameter horizontal pipes with no bends, and opening directly out
of the laboratory so that they can be cleaned from outside.
All surfaces in the testing laboratory, culture room and media preparation room
where dust could accumulate (window panes and sharp corners in the floor) need to
be made at an angle and curved.
No pipelines and conduits should be laid on the surface of the floor or along the
surface of walls in the laboratories as they permit dust and dirt to accumulate and
are impossible to clean. They should be embedded.
Storage cupboards for glassware and chemicals should be put up at appropriate
places above 1.6 meters on the walls in the testing laboratory, washing room,
sampling room and media preparation room (height 100 cm x depth 45 cm).
Tint the glass windows in the outer walls to prevent direct sunlight entering the
laboratory
There should be no ceiling fans in the testing laboratories and the ceilings should
be smooth.
Extraction fans may be fixed in washing and media preparation room, but kept
closed except during operation.
Enamel paints are preferable on the walls since they can be washed.
The proposed layout of equipment is given in Fig. 7.
FIG 5- LAYOUT PLAN FOR A MICROBIOLOGICAL LABORATORY OF
CAPACITY 130 sq. m.
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The key function of a microbiology lab is to provide a safe and efficient environment for the
culture, study, storage and disposal of micro-organisms. Addressing these requirements means
youve got yourself a lab.
Subsections:
Glassware Petri Dishes, Test Tubes, Flasks What to get and how to keep
Chemicals, Buffers and Reagents Commonly desirable chemicals and their uses
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Abdullah Ahmed at Sylhet MAG Osmani Medical College Hospital 1 year ago
KNOW WHAT YOU ARE DOING Laboratory medicine in general and microbiology in
particular is presently subject to rapid evolution. Do we know where we are going?
What are the driving forces? Is it good, bad or just plain necessary? Who is gaining
and who is losing?DR.T.V.RAO MD 4 View slide
BIOSAFETY LEVEL 1 Biosafety level 1 (BSL-1) is the basic level of protection and is
appropriate for agents that are not known to cause disease in normal, healthy
humansDR.T.V.RAO MD 11
BIOSAFETY LEVEL 2 Biosafety level 2 (BSL- 2) is appropriate for handling moderaterisk agents that cause human disease of varying severity by ingestion or through
percutaneous or mucous membrane exposureDR.T.V.RAO MD 12
BIOSAFETY LEVEL 4 Exotic agents that pose a high individual risk of life-threatening
disease by infectious aerosols and for which no treatment is available are restricted to
high containment laboratories that meet biosafety level 4 (BSL-4) standardsDR.T.V.RAO
MD 14
every one. However we certainly need new generation of Microbiologists to take up the
Tuberculosis related work.DR.T.V.RAO MD 23
DR.T.V.RAO MD 26
WHONET Working with WHONET make you familiarize with optimal drug choice,
zone sizes, easier to understand in interpretation in precise reporting.DR.T.V.RAO MD
32
CARING FOR SELF IS EQUALLY IMPORTANT Our health and disease is a concern
to us and family member apart from the society. All Bio hazardous micro- organisms and
materials to be handled with caution. Be a champion to promote the Universal
Precautions make the best contributions in prevention of infection in your own working
arena.DR.T.V.RAO MD 35
PUBLISHING YOUR WORK Always publish your genuine work in Journals and
periodicals; do not get disappointed if you cannot publish in a reputed journal. Now there
are good quality on line Microbiology Journals (e-Journals) and periodicals which are
indexed and available for your rescue. Every attempt to publish an article makes you
realize where we have to improve and by going through good references we can certainly
improve ourselves.DR.T.V.RAO MD 36
INTERNET AND E-LEARNING. All young and senior professionals should refer the
good potential of information high way, the Internet and get the best out it. E-learning
should be our next option to know what the world is thinking about. I consider it is the
best emerging option for improving our knowledge in Microbiology.DR.T.V.RAO MD 37
SHORTEN TIME TO DIAGNOSIS Transportation of samples -distance, service, 24havailability of blood culture cabinets. Availability -opening hours (24 h service, 7 d).
Analytical methods process time, random access Timeliness of reporting (and
making sure reports are looked at) Shorten time to susceptibility test results Rapid
detection of susceptibility Rapid exclusion of resistanceDR.T.V.RAO MD 45
THE WAY FORWARD The true symbiosis between large, well equipped and richly
staffed microbiological laboratories and smaller hospital based laboratories providing
close- proximity microbiology and consultancy services and the basis for infection
controlDR.T.V.RAO MD 46
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