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ABSTRACT
Cashew nut shell liquid (CNSL) a byproduct of cashew nut (Anacardium occidentale) by itself is useful
for insecticide, fungicide, anti-termite, and medicinal applications. Its insecticidal properties were
tested against two caterpillar pests in laboratory during 2007-09. The CNSL oil and partially
hydrogenated preparations were evaluated at 1% concentration for their insecticidal properties against
Helicoverpa armigera neonates by artificial diet surface incorporation method in no-choice assay.
Comparisons were made with the ideal botanical neem (active principle azadirachtin, 20% purity). All
azadirachtin treated neonates stayed in initial larval instars only leading to ultimate death. CNSL
treatments resulted in delayed larval and pupal periods and deformed larvae. Insecticidal activity was
recorded by stomach poisoning in diet-incorporation, but no contact toxicity by direct-spray method.
The CNSL preparations were also evaluated against Spilarctia bliqua (8 d old, laboratory reared on
castor leaves) following
direct-spray by potters tower, as well as no-choice leaf dip method. Few other botanicals
(azadirachtin, karanjin, jatropha aqueous extracts) were also compared. Significant insecticidal activity
could not be detected for botanicals in general. Both in direct-spray and leaf-dip bioassay, CNSL
was found ineffective against 8 d old larvae of S. obliqua.
Key words: Azadirachtin, cashew nut shell liquid (CNSL), Helicoverpa armigera, Spilarctia obliqua
Test insects
Helicoverpa armigera Hubner (Noctuidae:
Lepidoptera) larvae were reared individually in
borosil culture tubes (20ml) on artificial diet (Gupta
et al.,
2004) in environment simulation chamber (27+10C,
6070% RH, 16:8-h scoto/ photophase regime). The diet
consisted of host plant material (kabuli gram), yeast
powder, vitamin mixtures, anti-microbials and fixing
materials. The second test insect Bihar hairy
caterpillar
Spilarctia
obliqua
(Noctuidae:
Lepidoptera) was reared on natural food castor
leaves.
CNSL was obtained from Indian Oil Corporation,
Faridabad. Reduced CNSL was prepared by
hydrogenation of CNSL. Raw cashew nuts procured
from Bihar was processed with hexane to obtain the
hexane extract. Neem- azadirachtin (Aza, 20%
purity) was taken as standard. Water and acetone were
used as blanks. Thirty neonates were subjected to
treated food till their pupation and adult emergence.
122
Bioassay techniques
Diet surface incorporation method: Botanical
samples of CNSL were evaluated at 1% concentration
using no-choice bioassay against neonates (up to 12
h old) of H. armigera; Direct spray by Potters
Tower: Larvae (5 d old) were screened for their
contact toxicity by thin-film residual technique;
Leaf-dip method was followed for contact toxicity
tests taking castor leaves.
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1. Helicoverpa armigera
CNSL treatments resulted in delayed larval and
pupal periods and deformed larvae (Plate 1). In all the
CNSL treatments mild to moderate larvicidal action
was recorded (Table 1). Hydrogenated CNSL was
more active than CNSL and exhibited 75% mortality
till pupation as against 20% and 23.3% in aqueous
and acetone-blanks, respectively. CNSL based
products did not exhibit contact toxicity against 5d old
larvae by direct spray method or even in leaf dip
method (so data not given). Among the various
treatments azadirachtin was found to be the most
active. Initial lower larval mortality at higher
azadirachtin concentration, compensated by
higher mortality later clearly demonstrated its
repellent/ antifeedant action (Fig. 1). All azadirachtin
treated larvae stayed in initial instar stages only, and
none could reach pupation, ultimately leading to
death. As evident from the results, CNSL treatments
resulted in delayed larval and pupal periods
Table 1. Effect of azadirachtin (Aza) and cashew nut shell liquid (CNSL)
against neonates of Helicoverpa armigera
Treatments
2-d
3-d
4-d
5-d
6-d
7-d
9-d
Pupation
(days)
11-d
1-d
CNSL (1)
37.5c
43.8b
28.1ab
CNSL (2)
35.7c
50.0c
50.0c
57.1d
57.1c
57.1d
57.1c
60.7c
75.0c
35.7c
>17
CNSL (3)
3.2a
19.3b
25.8b
29.0bc
32.2b
35.5bc
38.7b
38.7bc
38.7b
3.2a
>17
Aza(100ppm)
20.7b
20.7b
24.1b
27.6b
31.0b
37.9bc
65.5d
82.8d
93.1c
20.7b
>17
Aza(250ppm)
pupation at all
13.8ab
17.2b
34.5b
34.5c
44.8c
48.3c
72.4d
75.9c
86.2c
13.8ab
No
Aza(500ppm)
7.1a
10.7a
14.3a
14.3a
42.8c
46.4c
64.3d
92.8d
92.8c
7.1a
Blank (aqu.)
13.3a
13.3a
16.7a
16.7a
16.7a
16.7a
20.0a
20.0a
20.0a
13.3a
Blank(acetone)
10.0a
13.3a
16.7a
16.7a
16.7a
16.7a
16.7a
23.3a
23.3a
10.0a
15-17
Figures following common letters (a, b, c) in rows are statistically at par by DMRT.
(1) CNSL samples from Indian Oil Corporation, Faridabad (2) Hydrogenated CNSL, and (3) Hexane extract (all 1%)
Insecticidal activity of cashew nut shell liquid against two lepidopteran pests
G. K. Mahapatro
123
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Larval mortality
Adult
till pupation (%) emergence (%)
1
2
Control
CNSL (1%)
0
20
68
35
Jatropha (1%)
20
50
Karanjin (1%)
25
35
Aza 100ppm
30
20
Aza 200ppm
35
25
Aza 500ppm
38
52
Aza 1000ppm
34
58
124
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ACKNOWLEDGEMENTS
REFERENCES
Davi, F. F., M. G. Cavalheiro, S. M. Viana, G. P.G. De Lima, L. C.
B.
Da Rocha-Bezerra, N. M.P.S. Ricardo, and A. F.U.
Carvalho.
2009. Insecticidal action of sodium anacardate from Brazilian
cashew nut shell liquid against Aedes aegypti. Journal of the
American Mosquito Control Association, 25(3): 386-389 (doi:
10.2987/08-5851.1).
Echendu, T.N.C. Ginger 1991. Cashew and neem as surface
protectants of cowpea against infestation and damage by
Callosobruchus maculatus. Tropical Science, 31: 209-211.
Gerold C.J., Mwalongo, L., L. Mkayula, B. Dawson-Andoh, E. B.
Hijau pestisida meskipun mereka moderat e f ficacy th e lates t Globa l tren d i n th e managemen t o f
agricultural pests . Amon g th e variou s botanicals , nee m and pyrethru m hav e s o fa r dominate d
th e scene. Nevertheles s considerabl e scop e exist s fo r the exploration keanekaragaman hayati
tanaman besar untuk basis pabrik baru d CRO p protectants. Cashe w nu t shel l liqui d (CNSL) adalah
sebuah serba guna hasil tambahan dari itu mete industr y Th e kacang. memiliki sebuah tempurung dari
tentang 1/8 inci ketebalan dalam yang saya s sebuah sof t mengasah y com b structur e containin g sebuah dar
k coklat kemerahan kental cair. Ini adalah itu pericap cairan dari mur mete. CNSL merupakan bahan baku
industri yang sangat penting yang telah mendapat menggunakan aneka. Selain Industria l nya kita e i n
polyme r industr y, ther e i s ruang yang cukup untuk pemanfaatannya dalam pengembangan obat,
antioksidan s d pestisida s (Ohle r, 1979; Echendu,
1991). CNSL telah dilaporkan untuk menjadi aktif terhadap storag e serangga s (Echendu, 1991), rayap
dan phytopathogenic jamur (Ohle r, 1979; Davi et al., 2009), dan larvisida untuk nyamuk (Yusuf, 1967).
CNSL juga dilaporkan e f fective terhadap rayap untuk perlindungan timbe r, alon e o r admixe d wit h
tembaga klorida (Gerold et l., 1999; V enmalar dan Nagarveni,
2005). Howeve r, itu s insektisida l activit y khususnya
agains t lepidoptera n OPT s i s kurang. Thi s pape r laporan
T est serangga
H elicoverp a armiger a Hubne r (Noctuidae: Lepidoptera) larva adalah dipelihara sendiri-sendiri di
borosil cultur e tabung s (20ml) o n artificia l die t (Gupt a e t l.,
2004) i n environmen t simulatio n chambe r (27 + 1 0 C, 60 70% RH, 16:08-h Scoto / photophase rezim). Itu diet terdiri dari bahan tanaman inang (kabuli gram), ragi
powde r, campuran vitamin, anti-mikroba dan memperbaiki materi. Itu kedua uji serangga Bihar berbulu ulat
Spila r CTI a obliqu a (Noctuidae: Lepidoptera) wa s dipelihara pada alam makanan gelindingan daun.
CNSL diperoleh dari Indian Oil Corporation, Faridabad. Mengurangi d SSP L wa s mempersiapkan
d dengan hidrogenasi CNSL. Kacang mete mentah diperoleh dari Bihar diolah dengan heksana untuk
mendapatkan ekstrak heksana. Neem - azadirachti n (Aza, Kemurnian 20%) adalah diambil sebagai
standar. W ater dan aseton adalah penggunaan d a s kosong. thirt y neonatus s wer e subjecte d untuk
diperlakukan makanan sampai mereka pupation dan dewasa eme r gence.
Bioassay teknik
Die t surfac e incorporatio n Metode:
Sampel botani dari CNSL dievaluasi pada
konsentrasi 1% menggunakan 'no-pilihan' bioassay
terhadap neonatus (sampai 12 jam old) o f . H
armiger a; D i r ec t spra y b y T Potte r 's
berutang r: Larva (5 d old) adalah disaring untuk
mereka hubungi toxicit y b y tipis-fil m residua l
teknik, Leaf-dip metho d diikuti untuk uji
toksisitas kontak mengambil jarak daun.
Resu L T S AN D PEMBAHASAN
1. Helicoverpa armigera
CNS L perawatan s resulte d i n Delaye d larva l dan periode pupa dan larva cacat (Piring 1). Dalam
semua perawatan CNSL ringan sampai sedang aksi larvisida wa s Recorde d (T abl e 1). Hydrogenate d CNS
L wa s lebih activ e tha n CNS L d exhibite d 75% mortalit y sampai pupation sebagai terhadap 20% dan
23,3% di air dan aseton-kosong, respectivel y. CNS L dasar d produk s tidak menunjukkan toksisitas kontak
terhadap larva tua 5d dengan langsung semprot metode atau bahkan di daun menukik metode (Jadi Data tidak
diberikan). Antara itu berbagai Perawatan azadirachtin ditemukan untuk menjadi yang paling aktif. Awal
rendah larval mortalit y a t highe r azadirachti n concentration, compensate d b y highe r mortalit y
late r clearly demonstrated nya aksi penolak / antifeedant (Gambar 1). Larva Semua azadirachtin dirawat
tinggal di tahap instar awal y onl, dan tak satupun bisa mencapai pupation, akhirnya mengarah untuk
kematian. Sebagai jelas dari itu hasil, Perawatan CNSL mengakibatkan di terlambat larv l dan kepompong
periode
oleh 2 d dan larva cacat (Piring 1). CNSL menunjukkan ringan insektisida activit y. Tabel S terhidrogenasi
CNSL dipamerkan lebih kumulatif toxicit y. Meskipun itu adalah jauh lebih sedikit aktif dari azadirachtin, di
pandangan dari -nya berlimpah-limpah
1-d
CNSL (1)
CNSL (2)
CNSL (3)
2-d
37.5c
57.1c
43.8b 28.1ab
75.0c 35.7c
3.2a
19.3b
25.8b
20.7b
20.7b
24.1b
27.6b
31.0b 37.9bc
65.5d
82.8d
17.2b
34.5b
34.5c
44.8c
48.3c
72.4d
10.7a
14.3a
14.3a
42.8c
46.4c
13.3a
16.7a
16.7a
16.7a
Kosong
(aseton)
13.3a
16.7a
16.7a
16.7a
Aza (100ppm)
10.0a
> 17
3.2a
>17
93.1c
20.7b
> 17
75.9c
86.2c
13.8ab
Tidak
64.3d
92.8d
92.8c
7.1a
16.7a
20.0a
20.0a
20.0a
13.3a
16.7a
16.7a
23.3a
23.3a
10.0a
15-17
Angka-angka berikut umum huruf (A, b, c ...) di baris adalah secara statistik di par oleh DM R T.
(1) CNS L sample s m fro India n Oi l Corporation, Faridaba d (2) Hydrogenate d CNSL, d (3) Heksan e extrac t (Al
l 1%)
2. Spilarctia obliqua
T mampu 2. Larva mortalitas dan dewasa eme r gence Bihar berbulu ulat di botani Perawatan (Larva 7d,
kontak kebisaan tes, Potte r 's tower)
S tatistical analisis tidak dapat dilakukan sebagai tidak sah karena inconsistenc y i n hasil, angka s ar e mea n o f 3 ulangan
(masing-masing mereplikasi, N = 30)
Furthe r studie s ar e memerlukan d t o entitas aktif identif y antara itu kolam dari diketahui dan
diketahui CNSL konstituen, beberapa di antaranya termasuk asam anakardat, cardols, cardanols, d
methylcardanol s (Mari dan Gouvan, 1999) (Gbr.2). CNSL adalah sebuah ekstensif mempelajari naturall y
occurrin g pheno l obtaine d produk sampingan sa selama pengolahan biji mete, dan berisi severa l phenoli c
senyawa. thoug h Sifat termiticidal CNSL dilaporkan oleh beberapa pekerja, insektisida tindakan terhadap
lepidoterans adalah langka. Laporan ini merupakan studi komprehensif CNSL terhadap dua importan t
lepidoptera n hama s o f agricultura l tanaman, yang menggambarkan -nya insektisida activit y.
1. Anakardat asam, 2.
Cardols,
3. Cardanols, dan 4.
Methylcardanols.
Gambar. 2. Tentu saja terjadi non-isoprenoid fenolik lipid dari mete
Th e autho r i s thankfu l fo r th e test-botani s (CNSL dan Azadirachtin murni) disediakan oleh D r. Suresh W
alia (principa l Scientist, Agricultura l Chemicals), dan laborator y facilitie s menyediakan d b y Head,
Divisio n Entomolog y, IARI, Ne w Delhi.
REFERENSI
Davi, F. F., M. G. Cavalheiro, S. M. V IANA, G. P. G. D e Lima, L. C. B.
D a Rocha-Bezerra, N. M. P. S. Ricardo, d A. F. U. Carvalho.
2009. Insektisida l actio n o f sodiu m anacardat e fro m Cashe Brasil w nu t shel l liqui d agains t Aede s aegypti. Journa l o f America n
Mosquit o Cont r o l Associatio n, 2 5 (3): 386-38 9 (Doi:
10.2987/08-5851.1).
Echendu, T. NC. Ginge r 1991. Cashe w d nee m a s surfac e protectants o f cowpe a agains t infestatio n d e b damag y Callosobruchus maculatus. Tr
opica l E Scienc, 31: 209-2 1 1.
Gerol d CJ, Mwalongo, L., L. Mkayula, B. Dawson-Andoh, E. B.
Mubofu, J. Perisai s d B. A. Mwingira. 1999. Preventin g termite attack . Environmentall y friendl y chemica l combination s of cashe w
nu t shel l liquid , sulfite d wattl e tanni n an d coppe r (II) chloride . G r een Chemist r y, Februar y 1999.
Gupt a G. P., Bira h A. d Ran i S. 2004. Developmen t o f artificia l diet untuk r mas s rearin g o f America n bollworm, Helicoverp a armigera. India n
Journa l o f Agricultura l Sciences, 7 4: 548-551.
Joseph, A. 1967. O n th e larvicida l actio n o f mentah e cashewnu t shel l minyak.
Cu r r en t E Scienc, 3 6: 433.
Ohle r, J. G. 1979. Cashe w Communicatio n 7 1. Departmen t dari Agricultura l Research, Koninklij k Institut e Voo r d e r t OPE n,
Amsterdum. pp.75-76.
Maria, L melakukan s S., d G. C. de Magalhae s 1999. Journa l o f Brazil
Chemica l Societ y, 1: 13-20.
V enmala r, D. D Nagarveni, HC. Tahun 2005. Evaluatio n o f copperised Cashe w nu t shel l liqui d d nee m oi l a s woo d pengawet. The Internationa
l Researc h Grou p o n W oo d Perlindungan. IRG / W P 05 30.368, In: 3 6 t h Annua l Meetin g, Bangalore, India. 24-02 Agustus April
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