Josephine L.

Kuo
57 Old Forge Rd, Home: (626)789-8161
Tuxedo Park, NY 10987 Work: (845)731-3604
josiekls@yahoo.com

OBJECTIVE:

Seeking a challenging position as medical practice researcher with a progressive organization which
will utilize my skills, abilities and education in biomedical fields. I am also capable handling product
management, operations, and purchasing.

WORK EXPERIENCE:

NYU School of Medicine - Dept. of Environmental Medicine Tuxedo Park, NY

Research Scientist Assistant – PI: Dr. Moon-Shong Tang 2008 – Present

• Studying the mutagenicity and sequence specificity of DNA damage rates by UV irradiation
and Acrolein-DNA adducts
• Analyzing toxicity of Chromium (VI) ligand-conjugated DNA adducts and subsequent
nucleotide excision repair
• Studying DNA repair capacity of bladder and prostate cancer cells via UvrABC and T4
endonuclease V damage
• Research objective is to understand specific mechanisms of DNA damage leading to cancers
and conduct high-throughput screens for inhibitory compounds
• Studying the mutagenicity and repair synthesis capacity of DNA damages by UVC irradiation
and Hydrogen Peroxide treatment on different stages of melanoma and bladder cells

Taiwan Kaohsiung Veterans Hospital - Genetic Engineering Laboratory Kaohsiung, Taiwan

Research Assistant - PI: Dr. Ming-Hong Tai 2006 - 2008

• Studied adenovirus-mediated gene delivery of Hepatoma-Derived Growth Factor (HDGF) to

liver and lung for melanoma therapy
• Studied HDGF upregulation enhancing the invasive capability and metastatic potential of
melanoma cell
• Manuscript in preparation: "Kuo LH, Huang HW, Tai MH. Functional studies of Hepatoma-
derived growth factor expression in melanoma”
• Studied adenovirus-mediated gene delivery of Hepatoma-Derived Growth Factor (HDGF) to
cerebrum for glioma therapy

Taiwan Kaohsiung Veterans Hospital - Genetic Engineering Laboratory Kaohsiung, Taiwan

Research Assistant - PI: Dr. Ming-Hong Tai 2004 -2006
• Studied adenovirus-mediated gene delivery of anti-HDGF DNA and HDGF-RNAi to cerebrum
for glioma therapy

I-Shou University - Physiology Experimental Course

Teaching Assistant Kaohsiung, Taiwan
• Trained laboratory staff, taught 90 undergraduates, improved course efficiency 2004
-2006
I-Da Hospital at Kaohsiung, Taiwan -Department of Emergency Room
Volunteer Kaohsiung, Taiwan
• Measure blood pressure, completed paper work for registration and translation 2004-2005

RELEVANT SKILLS:

Laboratory
• Cloning/ Virus amplification/ Virus purification/ Multiplicity of infection calculation
• Cell culture and infection/ Proliferation, Colony formation, Migration, Invasive, Adhesion
assay
• RNA isolation/ Real- time PCR/ RT PCR/ PCR/ Flow cytometry
• Immunohistochemistry/ Immunofluorescence/ TUNEL staining/ Gelatin Zymography/
Western blotting
• Animal models for carcinogenesis and metastasis
• Protein purification and isolation/ Plasmid purification/ Genomic DNA isolation and
Sequencing/ Tissue culture
• Mutation detection by DNA sequencing/ Host Cell Reactivation Assay (HCR)/ Repair
Synthesis Assay/ Mutation Assay
• Bacterial Culturing/ Electrophoresis
• Antibody Production and Purification

Software
• SPSS/ Sigma Plot/ Win MDI/ Photoshop/ Microsoft/EndNote/

AWARD & LEADERSHIP POSITIONS:

National Sun Yat-Sen University, Department of Biomedical

• Class representative vice president 2007

I-Shou University, Department of Biomedical Engineering

• Second conduct graduate of the year 2006
• Chief commissioner of service societies clubs 2005
• Outstanding College Youth, by China Youth Corps 2004
• Class representative vice president 2003

POSTERS & CONFERENCES:

Hsiao-Mei Kuo, Kuo LH, Liu LF, Chen CH, Tai MH. Hepatoma-derived growth factor
overexpression Enhances tumorigenecity in hepatocellular carcinoma 2008

American Association for Cancer Research Annual Meeting

Kuo LH, Huang HW, Tai MH. Functional studies of hepatoma-derived growth factor
Expression in melanoma 2008
 The Chinese Society of Cell and Molecular Biology

EDUCATION:
M.S., Biomedicine, National Sun Yat-Sen University (Taiwan) GPA: 3.83
B.S., Biomedical Engineering, I-Shou University (Taiwan) Salutatorian GPA: 3.8
Relevant Coursework: Genomics, Molecular Biology, Biochemistry, Physiology,
Biotechnology

WORKING STATUS:
Permanent residence (green card holder)

REFERENCES:

Available upon requires