Parameter to differentiate IDA & ACD FEP

Decreased in IDA
Increased in ACD
Hb SC Hb S, hb C, hb F
Sero test for german measles IgG
IgM
Latex agglutination
Complement fixation
MDS FAB
RA
RARS
RAEB
CMMOL
RAEBT
MDS WHO
RA/RCUD/RN/RT
RARS
RAEBT 1
RAEBT 2
MDSNU
MDS DEL 5Q
Indention stage start Metamyleocyte
Hb production begins Prorubricyte
Pink hb, blue rna, polychromatic erythroblast Rubricyte
Overall blue, polychromatophilia
Polychromatic red cell
Reticulocyte
Meg csf BM
In reponse to megakaryocyte mass in BM
Thrombopoietin Kidney, liver, spleen
In response to platelet demand in circulation
Haptoglobin Intravascular hemolysis
Extravascular
Variant lymphocyte plasmacytoid L Type 1
T cell differentiation Thymus
FAB cell line Cell morph = romanowsky
Differentiation = cytochemical
WHO Immunophenotyping = cell line
Cytogenetic = classify leukemia

Hemoglobin 4 heme, 4 globin
Sickle cell Decreased OFT
Resistant to malaria

spherocytosis Increased OFT
Increased autohemolysis
Major protein Spectrin
SEA ovalocytosis / stomatoctosis HE Resistant to malaria
Embden meyerhoff PK def
Pentose phosphate G6PD def
Hereditary stomatocytosis/xerocytosis Autohemolysis test : not corrected
Hereditary pyropikilocytosis Increased autohemolysis test
G6PD def Lyons hypothesis, male is resistant to malaria
Oslers disease Polycytemia vera
Pancytosis, panmyelosis
Normo normo, rpi>3 a) PRCA = N/N, rpi<2
b) DIC= micro/hypo
c) Sickle cell= N/N, rpi>3
d) AOTA
Vcs tech, wbc differential Coulter LH750
Size in rbc MCV
FAB ALL L1
Small, homogenous, children, PAS
L2
Large, heterogenous, adult, similar to aml ml2
L3
Large, homogenous, adult, children, burkitt, oil red


Hairy cell Pancytopenia, fried egg, trap,
Tell LGLL RA, FELTY SYNDROME
REED STERNBERG CELL Hodgkin lymphoma, giant cell, owl eye,
Lacunar cell Tumor cell, cytoplasmic clearing
Popcorn cell Multilobed nucleus, indistinct nucleoli
Sezary cell Cutaneous T lymphoma
Mycosis fungioides Sezary cell
Cerebriform nucleus
Patutrier microabscess
HAM acidified serum test PNH confirmation
(+) secondary aplastic anemia
Sugar water screening test 9-10% sucrose, hemolysis (+)
CD 45 Highest in
Lowest in
Embrionic Mesoblastic stage
Gower 1, portland, gower 2
Weak acetic acid dilute Rbc
Correct for nrbc & wbc Cell dyn abott
Basket cell

22% albumin, before smear, for leukemic,
destroyed neutrophil

Smudge cell 22% albumin,
Dense granules Serotonin, calcium , adp, atp
PTP 1week after platelet transfusion
Anti HPA1a
Bleeding 2-12 days after transfusion
NAIT ALLOIMMUNE
anti HPA1a
NAT Mom, SLE
decreased platelets in babies
Stormorken syndrome Platelet express phosphatidylserine without prior stim
ulation

Scott syndrome Abnormal calcium
Phospholipid scramblinh
Platelet cannot undergo flip flop effect
Cannot bind coagulation factor
Beta thromboglobulin
Pf4
Thrombospondin