8am-9:15am Jay Hodgsons Poster Session Science Building
Matthew Borders, Ronald Hayes, and Christina Miller, The Evolution Of The Gray Wolf (Canis Lupus). Mentor Jay Hodgson: CST The gray wolf (Canis lupus) is an important apex predator, as well as keystone species, in many habitats across North America, Europe, Asia, the Middle East, and north Africa. Having appeared in the Middle Pleistocene, around 781-126 thousand years ago, it has been shaped and is continuing to be shaped by many environmental and ecological changes, including the presence of humans. There are believed to be four lineages of the modern gray wolf, all arising from the Eastern Hemisphere. The widespread colonization of the species allowed for a great number of subspecies to arise and become key members of their respective habitats. While it has shown itself historically to be adaptive, the future of the gray wolf is uncertain, due to climate change, loss of crucial wilderness habitat, human encroachment and persecution.
Jalesia Horton, James Conner, and Caryn Nelson, Modern Day Horse Evolution. Mentor Jay Hodgson: CST Equus feres is the modern day horse. Both wild and domestic horses belong to this species. Currently, it is commonly held that the earliest ancestor, Hyracotherium, arose approximately 55 mya in the early Eocene era. Hyracotherium was a small forest animal that had distinctly different toe and teeth structure compared to Equus feres. There was a gradual evolutionary trend from Hyracotherium to Equus feres of a reduction/loss of the outer metacarpals and digits, elongation of central metacarpal and formation of a bony hoof. The teeth also a followed a trend of elongation and the premolar/molar developed complex patterns of ridges that were more geared to grinding food. Key transitional fossils that showed such trends were Hyracotherium (55 - 45 may), Miohippus (33 - 29 mya), Merychippus (17 - 11 mya), Pliophippus (arose approx. 15 may) and Dinohippus (arose approx. 12mya). Equus feres is unlikely to go extinct due to widespread domestic use as in agriculture, sports and general entertainment. To this day there is only one surviving true feral sub-species (never domesticated) which is Equus feres przewalskii or commonly known as the Mongolian Wild Horse. These feral horses did disappear from the wild due to loss of habitat in the 1980s but were reintroduced in 1992 from surviving zoo populations.
Erica Janocha and Richard Watkins, The Evolution of Man. Mentor Jay Hodgson: CST Humans have been evolving into fine-tuned organisms for millions of years. The group Australopithecus, one of the earliest ancestors of Homo sapiens, lived roughly 3.8 and 3.0 million years ago in eastern Africa, the birth place of the human race. The fossil record can be utilized to track human evolution among the Hominini group. The fossils show the development of bipedal movement, an upright posture and an ever increasing skull size, among many other developments. Eventually humans began to migrate out of Africa and came to occupy nearly every continent on Earth; becoming masters of the environment and other organisms. But what does the future hold for the human species? Other organisms have faded into extinction as environmental pressures proved too great to adapt. Present day humans have evaded such fate due to key evolutionary adaptations that allow him to circumvent all but the most drastic of environmental changes. Catherine Jones, Roshni Patel, and Yeni Jacob, Morphological Changes to the Modern Horse Mentor Jay Hodgson: CST The modern day horse, according to some, is one of the most majestic mammals to walk the Earth, but did they always look, eat and live where they are found now? The answer to those questions is simple, no. The modern day horse has undergone many changes over the last fifty-five million years. Changes to teeth structure, height and feet structure all point to evolution. These changes are evident in the transitional fossils, but the question is, why where these changes necessary? Was it climate change, predation, food shortage or something else? A change in the climate is the most likely ecological pressure that occurred that caused the ancestral horse to undergo adaptation. Due to the change in climate, resources, such as food, became limited, changing the niche of the species. This, in turn, forced the species to adapt to the environment; those that could not adapt died off. This would not only account for the teeth change but also the change in morphology. By becoming larger and faster than the competition, the survival rate of the species is higher. Another question is what does the future look like for the modern horse? Will there be something to push it to evolve again? If the climate changes in either direction, it will force the species to evolve and adapt.
Sasha Lesure, Brian Hall, and Raymond Johnson, The Evolution of the Trilobite to Limulus polyphemus, the Horseshoe Crab. Mentor Jay Hodgson: CST The trilobites first appeared in the Paleozoic era of the Phanerozoic Eon. They flourished in many types of oceanic environments such as shallow flats and reefs, deeper ocean bottoms and the water column as free floating plankton. Their closest extant descendent is the Limulus polyphemus, the horseshoe crab. The most notable characteristic change is its morphology. The trilobites body shortened and elongated throughout its evolution. The changes were made predominantly within in the segments and spines on the organism. This may be due to the theory of feel big, look small as a protective mechanism from predators. The trilobites extinction at the end of the Permian era was due to many variables. Today, Limulus polyphemus, is a vibrant and strong species. They are capable of surviving extreme temperatures, going extended amounts of time without eating, and changes in salinity. This is why they have survived for 250 million years. The future of this organism, per our research, is still uncertain at this point due to the strain from pollution, global warming, and over fishing causing a decrease in the number of spawning horseshoe crabs. Limulus polyphemus is an important food source for the long distant migrant shore birds. Its extinction could bring about a drastic change in the ecosystem.
Emily Mathis, Anthony Ravita, and Laura Jones, The Chambered Nautilus and How it Has Been Favored in an Evolving World. Mentor Jay Hodgson: CST The chambered nautilus has been in existence since the early Pleistocene, and has remained relatively the same for the number of years that has been living in earths waters. Through fossils, pictures, and scholarly sources the way in which the nautilus has survived and evolved has been examined and explained. The chambered nautilus differs from its other members in the class cephalopoda, and that is the reason we have looked into every aspect of this animal in order to explain how the chambered nautilus has been so close to its original form in an ever changing environment that it surrounds itself in.
Rafael Oropesa, Cody Struthers, and Nell Brennan, The Evolution of Whales. Mentor Jay Hodgson: CST We will be presenting the theory of whale evolution and the problems faced by modern whales in relation to their survival. Fossil evidence supports this theory, from their evolutionary roots (the artiodactyls which split from the mesonychians) to the modern day whales. While there is a line of organisms which shows the successive evolution of whales; these organisms are not direct ancestors of the modern whale, but rather offshoots of the whales lineage. They are all closely related however and show the evolution of the modern day whales skull and skeletal structures. The artiodactyls were omnivores which had begun to adapt to an aquatic environment and hunted both land and aquatic organisms along water shores. We can see the skull and skeletal evolution and adaptation of the whales evolutionary lineage from organism to subsequent organism starting from this point. Across the whales lineage, the modern day whales' large size and unique bone structures were evolved to support life in first a freshwater, then a colder marine habitat. The future of whales is uncertain. They have been hunted (some species to near extinction) as a source of meat and blubber. There has been a ban placed on whaling in 1986, but whales are still hunted in many parts of the world. Whales also consume vast quantities of food due to their immense size. This means that they are at a great risk for the bioaccumulation of pollutants such as mercury. Adam Parker, Chelsea Cheek, and Janie Ussery, The Evolutionary History of the American Alligator (Alligator missisippiensis). Mentor Jay Hodgson: CST Alligator missisippiensis is an ancient species of reptilians that has been thriving for around 230 million years. They inhabit all types of freshwater and brackish ecosystems including rivers, lakes, ponds, marshlands, swamplands, and estuaries. Currently they are only found in the Southeastern United States. One of the most successful reptilians worldwide, they prey on a multitude of organisms allowing them a broader niche and a certain degree of resistance to ecological pressures. The crocodylomorphs of the late Triassic gave rise to the American Alligator and we expect to see a large number of transitional fossils as evidence linking the alligator to its ancestors. By examining the current fossil record, effects of climate change, and anthropogenic effects such as habitat loss, we will describe how the American Alligator has become a modern day top predator in various ecosystems. Trevor Rawlinson, Chandler Goldman, and Young Vo Evolution of Cyanobacteria Mentor Jay Hodgson: CST Cyanobacteria are known to have existed as far back as 3.2bya and initiated an environmental change via carbon fixation. They used oxygen as an electron acceptor in their electron transport chains, but specifically the utilization of the antenna complex and to split water molecules, releasing oxygen gas as a byproduct into the Chlorophyll atmosphere. The release of oxygen by cyanobacteria drastically changed the conditions of the atmosphere and paved the way for the evolution of organisms capable of utilizing free oxygen gas. Because oxygen is a very efficient, high energy-yielding molecule, larger forms of life were able to evolve. Marine cyanobacteria have since diverged into five strains that are capable of surviving in many environments, some even quite extreme. Activity of cyanobacteria can provide a sanctuary to small marine organisms, but can also disrupt the feeding activities of crustaceans. Cyanobacteria will continue to shape the environment, both macro and micro, by its metabolic activities.
Jessica Young and Joseph Snooks, Elephant Evolution. Mentor Jay Hodgson: CST Although Elephant evolution is well known this research shows a clear representation of how the ancestors of elephants lived in their environments and evolved from a tiny mouse-like creature to the elephants known today. Elephants are unique in how large they have become and also their trunks that can be precisely controlled. Elephants have not done much more evolving in the past years and may stay how they are however, if they were to further evolve they may acquire a more sleek body plan or further trunk control. Most research about elephant evolution is scattered and vague, however, my group will explain why elephants have evolved to acquire certain traits of their ancestors and why other traits were not favored. By organizing all the previously known research about their ancestors and the changing environment a complete picture of how elephants came to be can finally be achieved.
POSTER PRESENTATION ABSTRACTS
Mahmud Abdallah and Morgan Greenlee Candida Mannan Activates TLR4-transfected Cells in a Cox-2 Dependent Manner Mentor Traci Ness: CST The yeast Candida albicans is often found on healthy skin and mucosa but can become pathogenic in immunocompromised individuals. Pattern recognition receptors on epithelial cells are responsible for initiating local immune responses to invading pathogens. Macrophages have been shown to bind mannans in the fungal cell wall through cooperative interactions between toll-like receptor 4 (TLR4) and mannose receptor (MR). Since most epithelial cells lack MR, we hypothesized that mannan could activate TLR4 in the absence of MR. We used human embryonic kidney cell lines stably-expressing human TLR4, MD2, and CD14, but lack MR. These cells (HEK-Blue hTLR4) are engineered to express secreted embryonic alkaline phosphatase (SEAP) following receptor-induced NFB activation. Lipopolysaccharide (LPS), a well-known TLR4 activator of NFB, triggers a strong SEAP response and was used as a positive control. HEK-Blue hTLR4 cells exhibited a dose-dependent response to purified C. albicans mannan with peak activation occurring at 40g/ml. LPS is a potent inflammatory stimulus which activates the cyclooxygenase (COX) pathway leading to prostaglandin synthesis. Indomethacin, a selective COX-1 inhibitor, had no effect on LPS- or mannan-induced SEAP responses even at high doses (80 M). Treatment of HEK-Blue hTLR4 cells with Celecoxib, a COX-2 inhibitor, significantly reduced SEAP activation by LPS and mannan in a dose- dependent manner. These data indicate that mannan binds and activates TLR4 and triggers the COX-2 pathway of prostaglandin synthesis. Future studies may help elucidate the role that the interactions between mannan and TLR4 play in the epithelial cell recognition of invading pathogens.
**Lindsey Allen, Brittany Pritchard, and Marjory Taylor, Group versus Individualized therapy on the Cognitive Communication Skills of Patients with Traumatic Brain Injury. Mentor April Garrity: CHP Our research seeks to answer the question: Are post-acute TBI patients who participate in group/holistic-style therapies more or less likely to achieve functional cognitive and communication skills than TBI patients who received traditional individualized intervention? We chose this question due to the increasing use of group therapy versus more costly individual intervention. Group therapy has been presented as a more economical way to deliver therapy services. Recent economic and political developments have necessitated cost cutting measures by insurance companies, service providers and government agencies. Beyond its economics advantages, is group therapy effective? To answer this question, we looked at the three components of evidence-based practice: external scientific research, clinical expertise/ opinion, and client/ caregiver perspective. We conducted a search and analysis of available literature including meta analyses. We also gathered a clinical consensus of individuals in the area of cognitive and communicative intervention for individuals post TBI. Three studies were identified as resourceful: Cicerone, K. (2011); Teasell, R. (2012); Goldblum, G. (2001) & Ownsworth, T. (2008). Results of the studies provided levels of evidence that proved clinically significant suggesting that group/ holistic intervention strategies and individualized intervention strategies can both provide beneficial outcomes on functional cognitive and communication skills. Group therapy as an intervention method can be as effective, and in some cases more effective, in rehabilitating the cognitive and communicative abilities in individuals with TBI. Group therapy may not only be a solution for insurance companies and other agencies to cut costs but can provide realistic positive outcomes. Alex Allison, Evidence of A Political Business Cycle in the Firearms Market. Mentor Michael Toma: CoLA This research presents evidence of fluctuations in the US firearms market created by the government in the form of federal legislation and a regime challenge in election. OLS time series analysis is used to investigate price level changes in small arms that are associated with the complete implementation of the Brady Handgun Violence Prevention Act of 1993, a Republican President being challenged for office in election, and the unusually high levels of uncertainty associated with Barrack Obama campaigning for, and winning the office of President. All else equal, The threat of losing Republican rule in the White House surprisingly is associated with a 1% lower inflation rate, and The Obama uncertainty factor accounts for 2% higher gun price inflation. The Brady bill did not have a significant effect on gun price trends. Firearms price inflation is also higher in the period following a return home of troops deployed overseas. Claudia Alvarado and Rebekah Robinson, Construction of a Single Nucleotide Polymorphism in the Human TLR4 gene. Mentor Traci Ness: CST Pattern recognition receptors (PRRs) are responsible for recognizing pathogens and initiating appropriate immune responses in many cell types. Toll-like receptor four (TLR4), a well-studied PRR, is the receptor for lipopolysaccharide (LPS) found in the outer membrane of Gram-negative bacteria. However, it has been demonstrated that the fungal polysaccharide mannan is also capable of binding and signaling through TLR4, but the details of this interaction are poorly understood. TLR4 Asp299Gly is a naturally-occurring polymorphism which has been shown to eliminate LPS-binding. Individuals with this polymorphism are highly susceptible to Gram-negative bacterial infections. Our lab is interested in determining if this polymorphism will also eliminate mannan binding and signaling through TLR4 in human cells. The goal of this project was to construct the TLR4 Asp299Gly polymorphism in the plasmid pUNO-hTLR04a using circular mutagenesis. A single nucleotide change (adenineguanine) was introduced which changed the amino acid at site 299 from an aspartic acid to a glycine residue. The success of our mutagenesis strategy has been verified by antibiotic resistance of bacterial transformants, PstI digestion of the isolated plasmids, and direct sequencing of the plasmid. Future studies will include transfection of the TLR4 Asp299Gly plasmid into a reporter cell line to test mannans ability to bind and activate this polymorphic form of the TLR4 receptor.
Angela Bain, Human Papillomavirus Mentor Denene Lofland: CHP This abstract provides a literature review of the human papillomavirus, the consequences of such and available vaccines. Many different cancers have been linked to the human papillomavirus (HPV). These include: cervical, anal, vulvar, vaginal, penile and oropharyngeal. HPV is easily spread from any genital area contact because it can sometimes be asymptomatic, and normal use of condoms alone will not always prevent infection. Girls, boys, women and men, both heterosexual and homosexual; are at risk of contracting HPV. At this time, there are vaccines available to protect females and males. These vaccines offer the best protection if all three (3) vaccinations are received as prescribed and the body has time to develop an immune response before any sexual activity. Unfortunately, due to the relatively new introduction of the vaccines, long-term data are not yet available. Studies have shown assumptions that the vaccines could reduce the mortality rate of cervical cancer by approximately 40%. When the vaccines are given in combination with pre-cancer screenings, that number could reduce the mortality rate by up to 60%. Controversies regarding state/school mandated vaccinations for HPV have limited the number of children vaccinated. Most of this criticism is due to unfavorable media exposure and costs to the public health care systems. Human papillomavirus is easily spread and can contribute to serious, even deadly consequences later in life. The recently created, FDA-approved vaccines have been shown to increase the bodys immunity to this virus. More studies and research will continue to be provided as the vaccines become more readily available and mainstreamed into our public health care system. Kirk Barber and Chris Hustead, Analysis of diatoms living in water fountains on the Armstrong campus: ecological interpretations. Mentor Jay Hodgson: CST Diatoms are a type of photosynthetic algae found in many environments. Essentially, they can live in a variety of habitats as long as they have light and water. For our project, we wanted to understand how diatoms utilized urban habitats, such as water fountains, that may mimic the natural environments in which these organisms evolved. We sampled two water fountains on the Armstrong campus weekly for 77 days during summer 2012. Within each water fountain, we sampled two different regions that had different conditions. One fountain had a water jet that mimicked a waterfall and a metal sheet that resembled a mist zone. The diatom community was significantly different between these two regions. This may be the result of physical separation between these areas within the fountain, different metal types, and/or different water conditions highlighting diatom sensitivity to habitat type. The other fountain had a rocky substrate and a metal trim. The diatom communities were significantly correlated between these regions. This may be the result of the diatoms not discriminating against rocks and metal as suitable habitats. Also, the species compositions were different between the two different fountains. Overall, this project showed that some diatom species are sensitive to habitat types while others may not be. Elizabeth Beagle and Nicholas Ingebretsen, Enzyme Stereospecificity in YDL124w. Mentor Scott Mateer: CST Many reductases are stereoselective, meaning they only produce one enantiomer. This is important because one conformation of an enzyme binding site will produce only one product from a substrate. The goal of this class project was to change the stereospecificity of the YDL124w enzyme. To accomplish this, the entire class has done site-saturation circular mutagenesis of residue 125 of the YDL124w gene, replacing a phenylalanine with all of the other amino acids. Our part of the class project was to replace phenylalanine with tyrosine and histidine. Both amino acids will likely cause a change in the binding site conformation, thus affecting the enzyme activity. This is because a neutral nonpolar amino acid, phenylalanine, is being replaced with a basic polar amino acid, histidine, or a neutral polar amino acid, tyrosine. The mutagenesis was confirmed by first performing a PstI/PvuI restriction digest to screen for the PstI cut site. The cut site would not be present in the mutated gene. Positive mutants from the digest were then sent off for DNA sequencing to confirm the results. Enzyme activity would have to be measured to see if stereospecificity had been changed. Altering the binding site of the enzyme will provide further evidence of stereoselectivity in the YDL124w gene. **Kelsey Blyudzhyus, Jocelyn Fawkes, and Kristi Kelly, Reminiscence Therapy in Dementia Intervention. Mentor Jean Neils-Strunjas: CHP Is Reminiscence therapy effective for maintaining cognitive and affective functioning when treating patients with dementia in comparison to no intervention? Reminiscence therapy consists of discussing memories and prior events with others. This type of therapy utilizes prompts unique to the individual such as familiar items from the past and photographs (Woods et al). In order to answer this question with current literature, we conducted a literature search. Three studies were identified: Okumura et al (2008), Azcurra (2012) and Wang (2007). These studies examined cognitive and/or affective outcomes for patients with dementia using a group intervention design in which one group received reminiscence therapy and control group(s) did not receive therapy. The three components of evidence based practice were considered: clinical expertise, current best evidence, and client values and preferences. The conclusion drawn from the studies indicated clinical unanimity in individuals receiving Reminiscence intervention reporting an improved quality of life. In relation to cognition, clients that received Reminiscence therapy had significant word recall compared to the control group. The results of the studies suggested that Reminiscence therapy led to significantly greater cognitive and affective function than no treatment. However, further research is necessary to examine its effectiveness compared to other non-pharmaceutical forms of therapy.
Brian Bonham, Should America move toward socialized medicine? Mentor Lara Wessel: CoLA The use of socialized medicine in the United States has been a topic of discussion for decades, and one of increased attention in recent years. This growing support is due to the United States, as an economy and a government, spending enormous amounts on healthcare that rank among the top globally. A longitudinal study of the U.S. socialized medicine mammoth, Medicaid, shows the potential costs and benefits of a national healthcare system. Quantitative results support that social medicine would, in the near future, cost more per capita and establish a higher quality of care; however, in the long run, the expense would be potentially more affordable for all social classes in America and present the realm of possibility for a higher standard of care from a utilitarian perspective. The masses should take notice of the predicament of our healthcare due to the large possibility that unaffordable care may become a problem to many citizens in growing years; therefore, socialized healthcare legislation should be considered for this reason. Eric Branch, Chiral Lactones. Mentor Brent Feske: CST Chiral lactones are biologically active molecules that are most commonly found in pharmaceuticals and as insect pheromones. We have developed a synthesis toward -keto nitriles using aliphatic aldehydes as the starting material and umpolung chemistry as the key step. Several -keto nitriles were screened for their ability to be reduced by a bakers yeast reductase library of twenty ketoreducatases. The enzyme that resulted in the best e.e. was chosen for the reaction scale up to give gram quantities of the chiral alcohol. The resulting hydroxynitrile was hydrolyzed followed by spontaneous cyclization to produce the chiral lactone. Using the ketoreductases as the key asymmetric step we have shown a synthetic strategy to a variety of chiral lactones.
Ashlee Brett, Strategies for Injury Prevention in the Military. Mentor David Lake: CHP Background: Non-combat related musculoskeletal injuries are a major concern to military personnel; they result in lost hours and impaired combat readiness. To allow soldiers to perform at their highest level it is necessary to reduce the number of these injuries that are sustained. This review aims to survey prevention methods which focus on strengthening and stretching and determine their efficacy in preventing lower limb musculoskeletal injuries in military personnel. Methods: Six databases were searched to find studies which examined the relationship between training protocol and injuries. Only studies which evaluated military personnel were included; studies involving other high level athletes were excluded. Nineteen relevant articles were found by these database searches. The studies were then rated using the PEDro scale and more confidence was placed in the results of studies which scored higher. Results: In the studies with active interventions it was generally found that there was a statistically significant relationship between strength training and decreased incidence of injury. However, stretching was not found to have a statistically significant impact on injuries. Conclusion: According to these studies training strengthening exercises and modification of training both provide a decrease in musculoskeletal injuries in military personnel. However, it was not conclusively shown that stretching had a positive effect on injuries. Therefore, to decrease the incidence of lost hours and decreased performance it is necessary to implement a modified training protocol which focuses on strengthening prior to high intensity physical activity. Amber Brisard, A review of the Hepatitis B Virus. Mentor Denene Lofland: CHP A Hepatitis B infection is caused by the Hepatitis B Virus (HBV). It causes irritation and inflammation to the liver. The HBV is transmissible through body fluids such as: semen, saliva, blood. People who have unprotected sex, share needles while using drugs, or get tattoos with unclean needles are just a few examples of people who are at risk of contracting the HBV. Symptoms of the HBV may not appear until six months after being infected. Some peoples immune system can fight off the infection some never get rid of it and result in cirrhosis of the liver. The test that monitor your liver when having chronic hepatitis B are Albumin level, prothrombin time, and other liver function test. Treating chronic hepatitis B can be as simple of an antiviral drug to as complicated as drug therapy and a liver transplant. Acute hepatitis does not require treatment and will go away on its own. There are vaccines available for this virus and are highly recommended for babies and for people who are at high risk. Kathryn Brown, Teaching Future Nurses to Educate Patients. Mentor Debbie Mulford: CHP The purpose of this project was to teach student nurses how to provide patient education using the Teach Back Method. Nurses play a vital role in teaching patients how to care for themselves. Research has shown that in many cases patient education is not properly carried out and that an estimated 80% of patients cannot later recall health related teaching for a variety of reasons. The Teach Back Method integrates assessment of patient comprehension and provides a flexible framework in which to prioritize teaching based on patient needs and nursing considerations. Increased patient comprehension is linked to better patient quality of life and lower health care costs. In my project, I developed and taught an in-service for nursing students at Memorial Hospital designed to define their role in patient education, introduce them to the Teach Back Method, and identify patient needs in teaching. Students attending the in-service took a pre- and post-test. Results from the tests showed that students initially held several misconceptions about patient needs. Following the in-service, students demonstrated a better understanding of their role and responsibility in the process of patient teaching, increased familiarity with the Teach Back Method, were better able to identify patient needs. Rachel Butler, Gordon Wery, Deondra Curry, and Christy Coberly, Number Priming on Performance Attainment. Mentor Angie Koban: CST Numbers have been used by administrators to differentiate versions of tests in academic settings. Changes in environment have been shown to alter performance attainment; therefore numbers used as test markers may affect achievement. The researchers endeavored to discover whether marking an anagram test with either a high (99) or low (49) number could affect performance. Participants were asked to read a short article which was followed by an anagram test comprised of fifteen anagrams marked with either a high or low number, and then given a questionnaire. The questionnaire included questions about the article, demographics, and if they were aware of the number as a manipulation check. The researchers hypothesized that those participants who received the anagram test primed with the higher number (99) would score better than those who received the test primed with the lower number (49). If the hypothesis is correct, then administrators of tests should consider an alternative method for differentiating tests so as not to preemptively affect students performance.
Emily Cattanach, The neurological effects of concussion in soccer at the collegiate level: A Systematic Review. Mentor David Lake: CHP INTRODUCTION/ PURPOSE: The goal of this paper was to critically assess whether concussions cause neurological effects in collegiate soccer players and the risk of sustaining such neurological effects. METHOD: Several databases were searched using the words concuss*, brain damage, head injur*, soccer or futbol, and college* or higher education or universit*. RESULTS: Thirty-nine articles were found. Nine articles meet the age and collegiate participation criteria. All studies dealt with only the acute effects of concussion. Of the nine articles used six were quasi-experimental, two were observational, and one was a randomized controlled trial. Six of the nine articles found that soccer players were significantly more likely to have neurological defects after sustaining a concussion when compared to controls. Of the three articles that did not find a significant difference, two involved direct assessment of the athletes and the third reviewed reports to the NCAA from the schools. It was found that concussions with neurologic impact were mostly secondary to player on player head contact rather than head contact with the ball, ground or goalposts. Additional differences were also seen differentially by player gender and player position. DISCUSSION/CONCLUSIONS: There is evidence that supports that soccer players are more likely to suffer neurological consequences from concussions than controls. However there have been no studies of the long-term neurological consequences of concussions in collegiate soccer players sustained while playing soccer. Shieeda Chatfield and Brittany Mallard, Effects of cognitive stimulation therapy and anti- dementia drugs on increasing the quality of life in dementia patients. Mentor April Garrity: CHP This study was conducted to determine if cognitive stimulation therapy or anti-dementia drug intervention was more effective for maintaining a better cognition and quality of life for people with dementia. Two interventions currently utilized for maintaining cognitive- communication and affective functions among patients with dementia are cognitive stimulation therapy and anti-dementia pharmaceuticals. Cognitive stimulation therapy is a brief treatment for people with mild to moderate dementia. Each session targets to aggressively motivate and engage patients with dementia; while providing them with an enhanced learning environment and social benefits of a group. A thorough review of the literature on this topic yielded four articles that reported on the effects of anti-dementia drug interventions and cognitive stimulation therapy interventions on dementia. Aguire et al., 2011 and Spector et al., 2011 compared the use of anti-dementia drugs and cognitive stimulation therapy intervention. Gardner et al., 2011 described the impact of cognitive stimulation therapy intervention on dementia clients and Tjia et al., 2010 described the impact daily medication has on dementia clients. Based on the results of the four studies reviewed here, cognitive stimulation therapy intervention will benefit and improve cognitive functions and quality of life for dementia clients over time more than anti-dementia drugs. Quality of life can influence verbal communication, motivation in therapy, and interpersonal relationships. Cognitive stimulation therapy improves the quality of life of patients with dementia by helping decrease the likelihood of depression by increasing the persons abilities to communicate and by restoring confidence through a supportive environment.
Stephen Cooke, Replication of a Spatial Learning Task Using Java. Mentor Ashraf Saad: CST The purpose of this study is to demonstrate the applicability of spatial learning research from cognitive psychology to the field of computer science and the shared value of interdisciplinary research. This was accomplished by comparing data obtained from human subjects in a spatial learning task to that obtained from a computer program running an analogous task. In a study with humans, participants were required to navigate bins situated in 5x5 grids in a virtual environment in order to locate four goals oriented in a randomly placed diamond pattern. To recreate this task with a computer, Java was used to develop a program that simulates the basic parameters of the aforementioned spatial learning study. After creating a program capable of performing the spatial learning task I was able to analyze the methods that were implemented in order for the program to learn the required diamond pattern. The methods by which the program was able to learn the associations between goals are relevant to cognitive psychology and the methods for storing the associations in memory are applicable to cognitive neuroscience. Future research using the programming developed in this study will involve programming robots to perform the same spatial learning task. Through this and future studies I hope to foster interest in cross- disciplinary work by bridging a gap between psychology and computer science. Morgan Connor, Metal Ion Uptake by Polyacrylate Hydrogels. Mentor Catherine MacGowan: CST The use of superabsorbent polyacrylate materials (e.g. hydrogels) for the remediation of heavy metal ions from water is an area of interest. Hydrogels are hydrophilic, lightly cross- linked polymer chains that have the ability to absorb up to 300 times their mass in water, depending on environmental conditions. The objective of our project was two-fold: 1) to investigate the interaction of superabsorbent polymeric materials with multi-valent metal cations (e.g. Cu 2+ , Ni 2+ , Fe 2+ , Fe 3+ and
Co 3+ ) and 2) determine the efficiency of the metal remediation by the hydrogels. For each of the trials, five hydrogel beads were measured for their mass and diameter prior to being placed in 20.0mL of a solution containing metal cations at concentrations of 25.0, 50.0, 100.0, and 150.0 parts per million (ppm). After a 24-hour adsorption period, the swelled beads were removed from the solution and measured for mass and diameter changes. The concentrations of metal ions in the remaining solution were determined using atomic absorption spectroscopy. Preliminary results indicate that the beads were very effective for metal remediation, and that the efficiency is concentration dependent.
Shannon Counihan and Mehul Sheth, Site Saturation Mutagenesis of YDL124Wp Protein. Mentor Scott Mateer: CST Site-saturation mutagenesis is a form of site directed mutagenesis in which one specific amino acid is mutated to every other possibility. Creating a mutation at a specific site will determine the importance of the specific amino acid at that particular position as well as its effects on the stereochemistry and chirality of the protein. In our experiment, the class is doing a site saturation mutagenesis on the YDL124W protein in which our group will be changing residue 125 from a phenylalanine to tryptophan and valine. YDL124W belongs to the aldose reductase superfamily, which reduce aromatic alpha-keto amides. If the mutation is successful, the PstI restriction site will be removed and the plasmid won't be digested and we will then screen for a positive clone by doing a PstI/PvuI digest. We hypothesize that the change from a phenylalanine to a tryptophan will have a greater change to the stereochemistry than the change to a valine due to the physical properties such as hydrophobicity, charge and size of the side chains. The change from a phenylalanine to a tryptophan will change the side chain from being hydrophobic to having a slight charge whereas the change to a valine will decrease the hydrophobicity as well as the size of the side chain. If our mutation is successful, the mutation will show the importance of phenylalanine at reside 125 when compared to tryptophan and valine as well as help provide the foundation for further mutagenic research. Zachary Crawford and Cassy Cooper, The Versa-Pencil. Mentor Priya Goeser: CST This product is based on the concept of a mechanical pencil with a redesigned lead intake mechanism. Repurposing the mechanical function of a drill chuck, the versa-pencil is adaptable for using multiple lead sizes like a chuck to a drill bit, unlike normal pencils that use only one lead size. There are several toothed prongs set inside of an angled guide with a threaded ring placed around all the prongs. The thread on the ring moves over the teeth on the prongs; this rotation forces the prongs to move in or out along the direction of the guide. The area enclosed by the prongs decreases as the prongs move out and vice versa as the prongs move in. This design allows the chuck to accommodate varying sizes of lead. The roles of each major part in the chuck mechanism are summarized below: Chuck guide - provides an angled path for the prongs to slide Four prongs - move up and down along the path to tighten or loosen grip on lead of different sizes Ring re-sizers - come together to fit on the teeth of prongs to move them up and down with the rotation of the ring Currently the design has been completed in SolidWorks and a prototype has been made. Further research is being done to develop a fully functional prototype
Nicholas Degroot, Pulmonary effects of dioxin exposure from burn pits in the war on terror. Mentor Douglas Masini: CHP Introduction: Although enlistees into United States military service are required one to be free of pulmonary disease, acute onset small airway disease was on the rise within the veteran population, particularly in those returning from deployment. In nations under which the war on terror (WOT) operated, burning waste within an open air pit was the primary method of disposal. This billowing smoke made us question if it could be a potential source of illness. Dioxin is a known product of low-temperature chlorinated material combustion, and the literature revealed these low-temperature conditions were reproduced in the burn pit waste disposal method. Problem statement: Could WOT veterans acute onset airway disease symptoms be explained by inhalation of dioxin, whose production was secondary to burn pit exposure? Review of the Literature: Dioxin production was increased in burn-pits with low combustion temperatures and higher chlorine material content, and dioxin may then become bound to particulate matter < 2.5 m diameter. Dioxin was lipid soluble, and its effect on CD34+ cell gene expression showed 221 gene modulations. Post-deployment rates of blood cancers such as leukemia, lymphoma, and chronic obstructive pulmonary disease have noticeably surged among those with significant burn pit exposure. Method: We examined multiple burn-pit studies that demonstrated and estimated dioxin production, particulate travel, presence in human tissue, recognition as a toxin, known symptomology and the possible materialization of those symptoms among the exposed. In the absence of directly measured data on burn-pit derived dioxin exposure, these known capabilities were compared to the plausibility of being reproduced under similar conditions. Uncontrolled temperature within WOT burn pits have greater potential to produce dioxin from chlorinated material disposed within, and particulate-binding (< 2.5 m) that allowed for prolonged atmospheric pollution, deposition after inhalation and alveolar exposure. Early reports gave tentative evidence that symptomology was becoming increasingly common in young, previously asymptomatic veterans of the WOT. The variables of short timespan since the start of the WOT (and the beginning of U.S. veteran burn-pit exposure) may not allow for short term recognition of dioxins health effects in this sample of the population. We noted that retention of dioxin within the human body after initial exposure would possibly explain later-in-life symptoms, and wide-ranging genotoxic qualities would produce equally wide-ranging symptomology. Conclusion: While more research was recommended, we found that that the literature supported our contention that service members who participated in burning, or lived next to burn-pits, could have been exposed to dioxin which has been responsible for atypical lung diseases.
Serina Doolittle and Deep Patel, Mutagenesis of F125. Mentor Scott Mateer: CST YDL124w is an enzyme that has been shown to be enantioselective for some carbonyl substrates. In the binding region of YDL124w, residues 123-125, the amino acid sequence encodes for serine, proline, and phenylalanine all of which contribute to the binding of a carbonyl substrate molecule to produce a stereospecific alcohol. During Cell/Molecular lab, the class preformed site saturation mutagenesis, a type of circular mutagenesis, to residue 125 (phenylalanine). The focus of this group is to modify phenylalanine at residue 125 to either a proline or glutamine residue. Mutating this residue could potentially change the stereospecificity of the enzyme YDL124w. Phenylalanine and proline have similar chemical properties, such as being nonpolar, that may lead to the binding regions of the enzyme being able to produce the same enantiomer as YDL124w Glutamine, however, is opposite in chemical properties compared to phenylalanine, it is polar, which may lead to the binding region to produce another enantiomer. A Pst1 and PVU1 Restriction Enzyme Digest will screen for the mutants. If the mutagenesis was successful then the Pst1 restriction site should have been eliminated. To completely verify that the correct mutation has been made at residue 125 the suspected mutant will have its DNA sequenced. By mutating the F125 site to proline and glutamine, a better understanding of how the binding region of YDL124w works can help predict the stereochemistry of enzymes.
James Duddleston and Amanda Murrell, A multi-proxy record of paleohydrogeology in a southeastern USA tidal marsh: diatoms and sediment composition. Mentor Jay Hodgson: CST We cored the sediments of Groves Creek near Savannah, GA, which is a marsh characteristic of southeastern USA tidal environments, for diatom frustule and sediment grain analysis. Our working hypothesis for this marsh is that it underwent hydrogeologic changes through time, and these changes would be evident in shifts in diatom community structure and sediment composition. The top 300 cm of the core was dominated by epipelic (attached to clay) and epiphytic (attached to plants) species of diatoms, and the lower 300 cm of the core was dominated by episammic (attached to sand) and haptobenthic species. The upper 300 cm of the core was primarily clay, with a maxima of 78% of the sediment composition, and the lower 300 cm of the core was primarily sand, with a maxima of 93% of the sediments. Diatom PC1 was significantly correlated with both % clay (r = 0.445, p < 0.0001) and % sand (r = -0.469, p < 0.0001). These results suggest that this environment transitioned from a shallower, sandy environment to a deeper, muddy environment through time. Patrick Ellington and Natasha Hall, Circular Site Saturated Mutagenesis on YDL124w. Mentor Scott Mateer: CST The YDL124W protein is a highly enantioselective alpha-keto reductase that uses NADPH to reduce achiral molecules to chiral molecules. In a class-wide project, circular site saturated mutagenesis was used to saturate position F125 on the YDL124w gene. In particular, our group mutated the F125 residue to glycine (G) and glutamic acid (E). By introducing these two mutations, the PSTI site in the 123-125 region was eliminated. A PSTI/PVUI digest was then used to screen the DNA samples and clones that were positive for the PSTI site elimination had their mutations verified by DNA sequencing. Measuring enzymatic activity post- mutagenesis has the potential to provide insight about the importance of the F125 residue to making enantiospecific chiral molecules. When mutating phenylalanine (F) to glutamic acid, a significant decrease in activity is suspected because of the vast difference in the chemistry of their respective side chains. However, a change from phenylalanine to glycine should not cause major activity differences because both amino acids have non-polar side chains which give them similar chemical properties. Once more is known about the nature of enantiospecific activity of enzymes like YDL124wp, the making of enantiomerically pure substances could be greatly benefitted.
Madison Esposito, The Meat Eaters Mouth Mentor Debbie Tucker: CST Purpose/subjects: This experiment will compare the effects of animal product based diets on the cheek cells, the salivary amylase production and efficiency, and the bacterial environment of the human mouth. The experimental subjects will have varying diets in relation to their intake of animal products. One subject will eat an average amount of meat and animal products, one subject will eat no meat but will eat animal products, one subject will eat neither meat nor animal products, and the final subject will eat twice the animal product intake of the average meat eater. Procedure: A saliva sample will be taken from the subjects twice daily for a week, once after brushing and rinsing with mouthwash and again after a midday meal. Petri dishes of sheep blood agar will be inoculated with the samples saliva. The cultures will be grown and the species and number of CFU of the cultures will be compared between subjects. The level of hemolysis of the cells will also be observed and compared. At the beginning and end of the week long experimental period cheek cells will be harvested from each sample and observed for swelling or other abnormalities. At the end of the week a 1 ml saliva sample will be obtained from each subject and then introduced to a 1 ml sample meal relative to the subjects diet. The sample meal will be tested for glucose levels before introduction of saliva and then again after introduction. Katharine Field, Samantha Cain, Jessica Ferguson, and Jenna Birch, Effect of Positivity on Memory Recall. Mentor Angie Koban: CST Feedback can be beneficial or detrimental in many different settings. For example, positivity in the classroom can motivate, encourage, and improve a students overall perception of their own capabilities. Previous research on feedback has suggested that positive feedback improves memory performance. Our study examined the effects of positive or doubtful comments on memory recall. There were three experimental groups, a positive comment group, a doubtful comment group, and a no comment (control) group. We examined these effects by testing the participants ability to recall specific details from a given article. The participants read an article and then were tested with a fact recall sheet and five multiple-choice questions. We hypothesized that we would see a significant increase in the amount of facts recalled and correct questions answered in the positive comment group, and a significant decrease in the doubtful and control groups. We believe our results may be able to create a more positive learning environment where positive feedback can be used to improve memory.
Steven Finnell and Joey Grasso, Coordinate Point Navigation for a Mobile Robot. Mentor Ashraf Saad: CST My research project consists of getting a robot to navigate to a certain point on a 5x5 grid by using the shortest route possible. By doing this we will be displaying an example of how programming and math form a type of union and increasing interest in programming as well as the robotics field. By using the built in thumbwheel as well as the built in screen, the robot prompts the user for its current orientation as well as the starting x and y coordinates and the end x and y coordinates. Using the data gathered from the user we will use a mathematical approach by treating the 5x5 grid as the first quadrant of a coordinate plane. By doing this we see that each intersection is identified by a coordinate. We take the user input and use the slope formula to find the distance we need to travel. This way if the starting coordinate is [1,0] and the end coordinate is [2,3] we know that the robot needs to travel one intersection over and 3 intersections up. To keep the IntelliBrain-bot from running into the white space, we plan on using the robots four line sensors. The way the line sensors work is that the robot sends out a signal which returns a voltage between 0 and 5. 0 being pure white and 5 being pure black then the robot will return a value from 0-1023 depending on the voltage it reads. This leaves a wide margin to identify whether a color is black and white. The robot follows the line using its inner line sensors by turning slightly right every time the left sensor picks up white and turning left every time the right sensor picks up white. The outer line sensors will be used to count the amount of intersections that the robot has passed by. This lets use tell the robot to go up or over until a specific amount of intersections have been counted. By combining the programming aspect of this project as well as the mathematical concept being used, a clear demonstration of the integration between math and programming through the use of an easy concept is demonstrated. Due to the ease of the math, students in middle school or higher can understand how the robot does what it does. This is the goal we are aiming for because our end goal is to promote students interest in the computer science field. **Abigail Foos, Brittany Hughes, and Ashleigh Hurd Comparing Inpatient and Outpatient Treatment for Reintegrating Military Personnel with Traumatic Brain Injury Back into the Community Mentor April Garrity: CHP According to the U.S. Department of Defense, traumatic brain injury (TBI) is the signature injury associated with the wars in Iraq and Afghanistan (www.defense.gov). We use two primary models of rehabilitation for our military with TBI. Inpatient facilities allow for intensive treatment, however, the patient is required to remain at the facility for extended periods of time. Outpatient therapy permits the patient to receive services for a short amount of time each day, and return home to their families. Given the differences in these models, we asked: Which of these models is associated with more positive outcomes for reintegrating military service members with varying degrees of TBI into the community? Our literature search produced three articles addressing this question. Braverman et al. (1999) discuss the use of an intensive 8-week inpatient TBI treatment program to target a variety of social, cognitive, and vocational areas. The Group Interactive Structured Therapy (GIST) is a 13-week, outpatient, group intervention for individuals with varying degrees of TBI, targeting better community functioning (Hawley & Newman, 2010). Trudel et al. (2007) provides a systematic review discussing various methods of intervention for members of the military suffering from a TBI, including inpatient and outpatient treatment. Results of these studies suggest that inpatient and outpatient methods are both effective, but family and personal aspects should be the deciding factor in determining which method to pursue. More research evidence about both of these models is required in order to prescribe optimal treatment to our military service members with TBI. Arianne Gauthier, Anthony Ravita, and Elise Santorella, Cellular localization of two Golgi tethering proteins. Mentor Sara Gremillion: CST In order to maintain proper Golgi function, this organelle must continually recycle membrane and enzymes lost by departing vesicles. The Conserved Oligomeric Golgi (COG) complex is a tethering complex involved in the retrograde transport, or recycling, of vesicles within the Golgi. Studies in animal and yeast cells revealed that the COG is an eight-protein complex with the essential subunits of COGs1-4. In the filamentous fungus Aspergillus nidulans, very little is known of the COG complex. Fusion PCR and cell transformations were used to create two new strains of A. nidulans with GFP-tagged versions of the homologues of COG2 and COG4. A punctate pattern of fluorescence was observed in each strain. These results are indicative of Golgi localization in fungi; therefore, the homologues likely carry out a similar function in A. nidulans as COGs of other organisms.
Catherine Gallahue, Juanita Velez, Lindsay Langton, and Kayla Martin, Correlates of Sub- Clinical Narcissism. Mentor Nancy McCarley: CST The goal of our study was to investigate the relationship between forms of narcissism and both compulsiveness and dissociation. Given that grandiose narcissism includes repression of negative representations (of self and others) and distortion of disconfirming information (Pincus & Lukowitsky, 2009), we hypothesized a positive correlation between grandiose narcissism and dissociative experiences. Because vulnerable narcissism entails a lack of engagement in compensatory behaviors that effectively ameliorate self-doubt, social anxiety, and hypersensitivity, we anticipated a negative correlation between vulnerable narcissism and compulsiveness. The participants were 379 undergraduate Introduction to Psychology students who received research credit for completion of the online survey. Narcissism was measured using the Pathological Narcissism Inventory (PNI; Pincus et al., 2009). Additional personality traits were assessed using the Dissociative Experiences Scale (DES; Carlson & Putnam, 1993) and the Compulsiveness Inventory (CI; Kagan & Squires, 1985). Pearson correlation coefficients were computed among the PNI subscales, CI, and DES. As predicted, significant correlations were obtained between grandiose narcissism and DES scores (r = .354, p < .001, 2-tailed) and between vulnerable narcissism and CI scores (r = -.248, p < .001, 2-tailed). Although the hypotheses were confirmed, two concerns emerged. First, the overall magnitude of the relationships was modest. Also, we did not find a differential pattern of associations among the scales. Thus, dividing narcissism into subtypes would appear to have limited utility vis a vis compulsiveness and dissociation. This is consistent with Ronningstams (2009) observation that the narcissistic individual may fluctuate between assertive grandiosity and vulnerability (p. 113). Natasha Hall, Bilateral Modeling of Jaw Force and Skull Kinetics. Mentor Austin Francis: CST Migration of the eye during development contributes to bilateral asymmetry in flatfishes. Functional consequences of this morphological asymmetry were examined in the gulf flounder, Paralichthys albigutta. Seven gulf flounder were collected from the Florida coast (Atlantic and Gulf of Mexico) by hook and line or seine net in 2001-2002. Fish were preserved in 10% formalin for approximately one week and later transferred to 70% ethyl alcohol. To model the feeding biomechanics, gulf flounder were dissected to reveal the jaws and muscles of the head. Important biomechanical landmarks of the ocular and blind sides were digitally photographed and measured using image analysis software. Measurements included opening and closing in- levers, the lower jaw out-lever, adductor mandibulae II muscle length, and mandible length. The adductor mandibulae muscle was subsequently extracted, divided into the three principle divisions (I, I, and II) and weighed. These measurements were entered into the biomechanical modeling software MandibLever to generate predicted torque, gape velocity, and angle velocity. The modeling results were used to compare jaw force and skull kinetics of ocular and blind sides. For six of the flounder, most modeled variables were not significantly different between sides. One exception was effective mechanical advantage, which indicated the blind side consistently demonstrates greater mechanical advantage. Additionally, one small flounder (13 cm SL versus an average of 22 cm SL) exhibited asymmetry across a number of variables. As a result, future work will increase the sample size of smaller flounder to examine ontogenetic changes in feeding performance.
Ronald Hayes and Scott Sadler, Mutagenesis of YDL124w. Mentor Scott Mateer: CST YDL124W is a NADPH-dependent alpha-keto amide reductase used to make chiral alcohols. Enantoselectivity refers to the production of one enantiomer over another. Enantoselectivity is also important because enantiomers are chemically the same, but the optical rotation and functionally divergent; this is extremely important when pharmaceutical industries make new drugs for human health purposes. The class is using circular mutagenesis to perform site saturation at F125 (phenylalanine 125) in the YDL 124W gene. Our group is responsible for mutating the F125 to either an Arginine or a Serine residue. When compared to Phenylalanine, Arginine introduces a more bulky side chain that is positively charged and basic, while Serine is smaller with a polar side chain. By changing the F125 site to the intended residues we will determine enzyme stereoselectivity to prefer one specific enantiomer configuration allowing for an enzymatic reaction to happen. Our mutagenesis will eliminate the F125 site so in order to screen for our mutant clones we will use the restriction digest with the enzymes PstI/ PvuI and further verify changes by DNA sequencing.
Terika Howard, The Rubeola Virus. Mentor Denene Lofland: CHP The Rubeola virus is commonly known as measles. It is a member of the Paramyxovirus family and contains a haemagglutin protein in the lipid bilayer. The Rubeola virus is antigenically stable as well. It is a respiratory virus that grows in the cells of the lungs and throat. The virus mode infection is through droplets in the air or by direct contact with infected nasal discharge from people. Due to vaccination in the United States, the number of reported cases of the Rubeola Virus is on the decline, as opposed to other parts of the world. The Rubeola virus presents itself as a rash, cough, high fever and can cause encephalitis. Testing for Measles includes; serological testing, PCR, and isolation of measles. Preventative measure for the Rubeola virus is through live and inactivated vaccination.
Lara Kemp, American Alligators: The History of Hunting and Conservation in the American South. Mentor Michael Hall: CoLA This study was chosen as my main research paper for my History 3500 course, and I believed that this would be interesting and informative subject. I have mainly done my research by reading scholarly articles on the American Alligator, and I have also contacted DNRs from Southern states to see what information they could provide on their conservation efforts and their restricted huntings. I also have contacted groups who are part of the removal of nuisance alligators and how they remove them from the area they are in and what happens to them after their removal. My findings so far have been that all the Southern states have their own specific ways of dealing with the removal of nuisance alligators and that their restricted hunting seasons varies per state. My findings have also shown that a lot of people in the past believed that the alligators where monsters created by the devil because of their looks which is one of the reasons why they were hunted so ferociously, another reason that I have found is that people liked the looked of tanned alligator hides for fashion purposes. Stephen Kennedy, Synthetic Efforts toward New Bis(pyrazolyl)alkane Ligands. Mentor Brandon Quillian: CST Literature precedence has shown that minor changes to the surrounding environment of an organometallic complex can heavily influence its reactivity. Indeed, the simple conversion of a proton to a methyl group can either enhance or inhibit certain reactivity. We have prepared Ru(II) complexes with bis(pyrazolyl)acetate (BPA) as support ligands as precursors for the preparation of olefin hydroarylation catalysts. We now are interested in preparing similar support ligands with methyl substitution at the methine position to examine its coordination chemistry with ruthenium and to mask the acidic methine proton of BPA, which may be problematic during future studies. We describe in this presentation our current efforts and future strategies to prepare 2,2-bis(pyrazolyl)propionic acid.
Lia Kerkes, Gene Expression in Spanish Moss. Mentor Robert Gregerson: CST Objective: The objective of the project is to measure expression of a set of genes associated with nutrient assimilation in Spanish moss. Methods: RT-PCR was used to qualitatively measure gene expression. We used a variety of primers to measure the expression of multiple genes followed by gel electrophoresis to visualize the PCR products. Conclusion: We cannot determine what the mode of regulation is at this time but will continue researching.
Justin Kriske, Mapping the Shores of MATLAB: Exploring Beginners and Advanced Programming. Mentor Priya Goeser: CST This work presents recent updates to MatLab Marina, a Virtual Learning Environment (VLE) dedicated to teaching programming concepts using MATLAB. The VLE now includes a number of programming concepts that have instructional multimedia tutorials especially addressing particularly difficult concepts. Recent efforts have been focused on completing the content and number of tutorials available for the freshmen programming course Computing for Engineers (ENGR 1371). The various topics included are cell arrays, structures, functions, and iteration. A few advanced programming concepts such as nested functions, integration and roots of equations that are extensively used in a sophomore course Computational Modeling (ENGR2010) have also been included. The tutorials are created to be viewed in five minutes or less, with video, audio, and captions used to quickly and effectively explain concepts in a multimedia experience. The tutorials are developed using various software: PowerPoint and MATLAB for constructing the visual aids; Natural Reader for audio; Camtasia for synthesizing the visual and audio components to produce the final video; and the tutorials are housed on YouTube with links from the corresponding website. MATLAB is used by engineering students and professionals globally and so building effective instructional material is beneficial to this community. Currently there are a total of 65 tutorials, with a total of 57,693 views, with 22,164 views within the U.S. which shows their extensive use locally and globally. This and other assessment statistics show that MatLab Marina is being used and has significantly improved student learning of programming concepts.
John Lamb, M Walker, and C. Weed, Detection of Fusarium solani in Failed Loggerhead Sea Turtle (Caretta caretta) Eggs from Jekyll Island, GA. Mentor Jennifer Bailey: CST Loggerhead hatch success is significantly lower in Georgia than for sea turtles globally. Microbial infection represents one explanation for why eggs fail to hatch. The fungus Fusarium solani has been implicated in the death of loggerhead eggs on Boavista Island (Cape Verde, Africa), a major global nesting site, and has been detected in failed eggs collected from Australia. The potential involvement of F. solani in loggerhead embryo mortality in coastal Georgia has not been explored. In 2010, failed loggerhead eggs were collected from northern, central and southern coastal regions of Jekyll Island, GA. DNA was extracted from aseptically collected egg fluid. Thirty eight DNA samples were then screened by PCR using fungal-specific primers (ITS5F and ITS4Rev) that amplify the internal transcribed spacer (ITS) region. With each PCR run, a positive control (Fusarium solani genomic DNA) and a no template control was performed. Products formed from 18 samples, 11 of which were cloned and sequenced. While members of the fungal genera Penicillium, Capnodiales, Aspergillus and Dothideales were present, sequences corresponding to Fusarium solani (99-100% identity) were the dominant type recovered (77% of 61 sequences). Four out of the six nests screened had at least one egg containing F. solani. While it was not known whether this organism contributed to embryo mortality in the sampled eggs, this is the first known report of F. solani in North American loggerhead eggs. Haley Lattke, Colombia: The European Unions Attempts at Conflict Resolution through Official Development Aid and Peace Laboratories Mentor Jose daCruz: CoLA For more than fifty years, Colombia has been ravaged by a violent internal armed conflict. Illegal armed groups such as the Revolutionary Armed Forces of Colombia (FARC) and the National Liberation Army (ELN) are guilty of systematic human rights and humanitarian abuses against the civilian population in Colombia. Such atrocities have attracted the attention of prominent international actors, including the European Union. This paper examines the intervention efforts made by the European Union since the early 1990s to build lasting and sustainable peace in the Republic of Colombia. These efforts include, but are not limited to, Official Development Aid and Peace Laboratories as the primary instruments for carrying out the objectives the European Union hopes to achieve through its aid to Colombia. Through its Official Development Aid and Peace Laboratories, the European Union, in conjunction with the Colombian government, has been able to alleviate the conflict at the local level and help set the foundation for a more peaceful and stable Colombia. Although efforts made by the European Union have certainly yielded positive results, they have also had their shortcomings. This proves the need for further peace building initiatives outside of the areas targeted by the European Union.
Christiaan Layer, Dominque Walker, and James Parker, Effects of Note-Taking on Lecture Comprehension. Mentors Joshua Williams and Nancy McCarley: CST Note-taking has been one of the most prominent pillars of student learning. Traditionally, students construct their notes in real-time during lectures. Past research inquired whether it would be more beneficial for students to wait until after lecture to devise notes. Would doing so increase short term and long-term retention of information? In this study, three introductory psychology courses listened to a lecture under three conditions: note-taking in real-time, delayed note-taking, and no note-taking at all. Each condition was given a consolidation period of ten minutes after the lecture, during which students were to actively engage in the information they received. The short-term retention was assessed using a quiz that immediately followed the consolidation period. In a month, the same quiz will be re-administered to assess long-term retention. It is hypothesized that the students under the delayed note-taking condition will exhibit greater long-term retention because they were able to actively engage in the lecture material without the distraction of writing notes in real-time. They also had a second opportunity to actively engage in the information by constructing their notes after the lecture.
Renee Louis, Philip Rapillard, Samantha Galbraith, Annastacia Laing, and Lilianny Stephenson, Does the Presentation of Music Affect Memory? Mentor Angie Koban: CST Research demonstrates that adults with musical training have better verbal memory and more developed cognitive functioning than adults without musical training. Music, when paired with advertisements, creates favorable associations and enhances the memorization of product information. This musical pairing could be useful for scholastic learning. Our experiment was designed to examine the effect of music on memory. Three conditions were used to manipulate memory: lyrics spoken alone, lyrics spoken with background instrumental, and lyrics presented in song form (i.e. sung with background instrumental). Participants then listened to an audio clip of one of the three manipulations. They then completed a questionnaire designed to measure memory recall. We hypothesized that information presented in song form (both sung and spoken) would promote significantly higher memory recall than information being spoken without music. If our results show a significant difference in the amount of information retained with music compared to without music, than it would be logical to assume that music is capable of enhancing memory. Educators should therefore consider implementing coursework and lessons that involve music. Sami Mastrario, W. E. B. Du Bois and the Wings of Atlanta. Mentor Mary Barr: CoLA My poster presentation is on my attendance to the W. E. B. Du Bois and the Wings of Atlanta 50 Anniversary Commemorative Conference. My poster will focus specifically on the bust dedication ceremony of W. E. B. Du Bois. It will show prominent speakers at the dedication including W. E. B. Du Bois great-grandson Mr. Arthur McFarlane, project manager Stephanie Evans, and the sculptor, Professor Ayokunle Odeleye. The poster is also going to have detailed images of the bust and analysis in how it portrays Du Bois.
**Patty Mattingly, Lindsey Bartlett, and Annie Heekin, Group Therapy in Individuals with Chronic Aphasia. Mentor April Garrity: CHP Clinical Question: Aphasia is a disorder resulting from damage to the language centers of the brain. Two primary models of aphasia intervention include individual services and services rendered in a group environment. In order to determine which of these models has greater clinical efficacy, we asked: Do individuals with chronic aphasia who attend group therapy see greater improvement in communication skills and quality of life when compared to those who do not engage in group therapy? Methods/Procedures: A literature search yielded four articles addressing communication skills and quality of life among individuals with aphasia who participated in-group intervention models. Schoten et al. (2011) and Vickers (2010) focused on research collected from individuals who participated in group therapy and completed patient interviews and questionnaires about perceived quality of life. Outcomes: Group intervention techniques were effective in improving both communication skills and various measures of quality of life. Specifically, Schoten et al. (2011) and Vickers (2010) found positive psychosocial outcomes reflected in group therapy participants increased involvement in community activities. Conclusion: The articles suggest that individuals with chronic aphasia who participate in group intervention showed greater improvements with regard to communication skills and in various aspects of quality of life when compared to individuals with aphasia who do not engage in group intervention. Daniel Meis, The Effect of Fire on Florida Sand Skink Effective Population Size. Mentor Aaron Schrey: CST The Florida Sand Skink (Plestiodon reynoldsi) is precinctive to the Florida Scrub, one of the worlds most threatened habitats. The Florida Sand Skink is threatened because of their close association with this disappearing habitat. An additional concern for conservation and management is that the Florida Scrub is maintained by infrequent fire. These fires are known to affect genetic characteristics of Florida Sand Skink populations. We characterized the effect of fire on the effective population size of Florida Sand Skinks (n=611) from 13 locations at the Archbold Biological Station. Each location has a well-documented fire history dating back approximately 70 years. We screened 7 microsatellite loci and used two statistical methods to estimate the effective population size. The effective population size was maximized in locations that had not been burned for 10 to 25 years. Also, there was a negative correlation between the total number of fires in a location and the effective population size. Our data suggest that Florida Sand Skink populations may benefit from infrequent fires, but they may be negatively affected by multiple fires in the same location. However, we note that there are multiple species that use the Florida scrub habitat and each may prefer unique fire return intervals. Oneida Muniz and Jalesia Horton, Altering Enantiomeric Specificity: Circular Mutagenesis of YDL124Wp Reductase. Mentor Scott Mateer: CST Certain types of mutagenesis provide various pharmaceutical advantages including fewer adverse effects and lower doses. The purpose of our work is to change the enantiomeric specificity of the Yeast Reductase YDL124Wp by mutating the Loop A active site of the enzyme at F125. The active site of this enzyme interacts with carbonyls reducing them to chiral alcohols. Our enzyme is so stereospecific that it usually produces one enantiomer alcohol from a carbonyl substrate. By mutating the F125 site we are aspiring to find out if changing the amino acid will change the enantiomeric specificity of this enzyme. As a class we will perform circular site-saturation mutagenesis of YDL124Wp by saturating position 125 with every other amino acid. My partner and I will alter the enzyme by replacing F125 with either a Lysine or Isoleucine. If mutation occurred, the Pst1 cut site should be eliminated in the clones. We will verify if the appropriate mutant was made via a PstI/PvuI restriction digest and DNA sequencing. We speculate that the mutation from Phenylalanine to Isoleucine will not produce significant change in structural integrity because both molecules are relatively bulky and nonpolar. However the mutation to Lysine is expected to produce significant change since it is polar charged and is capable of ionic and hydrogen bonding. During the course of this experiment we will determine if by mutating F125 we can change the enantiomeric specificity of this enzyme and as a result produce the other chiral product that is not typically generated. Megan Netherland, Population Genetics of the Peninsula Crowned Snake. Mentor Aaron Schrey: CST The Peninsula Crowned Snake (Tantilla relicta relicta) is a fossorial species that is adapted to xeric upland habitats in central Florida. This habitat is more commonly known as the Florida Scrub region, which boasts very diverse reptile inhabitants and is also under severe threat due to commercial development. Very little information exists about this species and with the potential need for conservation we have endeavored to learn more about the Peninsula Crown Snake population. To provide important information about this species, we investigated the genetic characteristics across its range. We screened genetic variation of a portion of the mitochondrial cytochrome-b gene and RAG-1 gene in 60 Peninsula Crowned Snakes collected from 13 Lake Wales Ridge sites. Multiple PCRs were run in order to amplify the desired genes in the mitochondrial DNA and once obtained the samples were sequenced at the UGA genome facility. The sample sequences were then compared using multiple phylogenetic software packages. We detected population structure among geographic locations and identified a phylogeographic pattern that was similar to other Florida scrub reptiles. This suggests the various reptile species have inhabited the same geographic region for many generations and have perhaps evolved together based on their common environmental conditions. These finding will provide a foundation for future research on this species, especially should the need arise to conserve the species. Y. Tram Nguyen and Benjamin Oliver, YDL124w: Expression, Purification, Crystallization. Mentor Mitch Weiland: CST The aldo-keto reductase (AKR) superfamily comprises several enzymes that catalyze many redox reactions by converting carbonyl functional groups to alcohols. One general structure feature of these enzymes is the adoption of an (alpha/beta) 8 -barrel structure, which represents a compact but adaptable scaffolding suitable for a wide range of carbonyl substrates. Currently, there are 115 members in the superfamily that comprise 14 different families. One such protein, encoded by the yeast reductase gene YDL-124w, is an NADPH-dependent alpha- keto amide reductase that functions to reduce aromatic alpha-keto amides, aliphatic and aromatic alpha-keto esters to alcohols. Previous studies have utilized recombinant YDL-124w with a GST fusion tag to study protein function. Current efforts focusing on the expression, purification, and crystallization of the YDL-124w protein will be described. Ryan Olliff, Using Biocatalysis to Produce Chiral, Biologically Active Lactones. Mentor Brent Feske: CST Lactones are cyclic esters found in a number of biologically active compounds, including the cholesterol-lowering drugs Lovastatin and Simvastatin. Lactones are also found in nature as chemical signals among other things, including the beetle pheromone (R)--caprolactone. We have shown the production of enantiomerically pure lactones utilizing biocatalysis. Various aromatic aldehydes were reacted to the corresponding ketones through a number of organic syntheses. A library of 20 bakers yeast enzymes over-expressed in Escherichia coli was then screened for the enzymes ability to asymmetrically reduce the ketones to alcohols. The enzyme affording the greatest enantiomeric excess was then chosen for scale up to increase yield and the nitrile was hydrolyzed to afford the asymmetric lactone. Since these reactions were performed with enzymes, the need for harsh solvents, reactants and heavy metal catalysts was eliminated. Nicole Ormand, Dentition Patterns Found in the Upper and Lower Jaws of the Southern Flounder, Paralicthys lethostigma. Mentor Austin Francis: CST Flatfishes are well known for their asymmetrical characteristics, the most common is that of having both eyes on the same side of the body. Many species of flatfishes have been known to exhibit a difference in both the number and size of teeth on either side of the head. However, not much is known about the dentition patterns of the southern flounder, Paralichthys lethostigma. To better understand the function and occurrence of dental asymmetry in flatfishes, both the number and size of teeth were examined in the southern flounder. 28 fish were dissected to examine the upper and lower jaws of both eyed (ocular) and blind sides of the head. Each jaw element was cleaned and dried to better reveal the teeth. Each jaw element was then digitally photographed. Number of teeth was counted by hand and image analysis software was used to measure the length and width each tooth. A comparison between ocular and blind sides of the head did not reveal any significant difference in the number of teeth, the length of the teeth, or the width of the teeth for either the upper or lower jaws. Comparing the average teeth length for blind and ocular sides for both the upper and lower jaws shows a slightly higher length in the blind sides. However, this difference is very small and may not be a significant when comparing the two sides. The absence of asymmetrical dentition is probably the result of their diet. The southern flounder typically eats crustaceans and shrimp, which walk along the bottom and smaller fish. Because their prey size is smaller and may not be as quick as some other type of prey, differences in blind and ocular sides for feeding may not be essential to catch their prey.
Erin ORourke, The Therasuit Method: A Systematic Review. Mentor Dr. David Lake: CHP INTRODUCTION/PURPOSE: Cerebral palsy (CP) describes a group of disorders of movement and posture that cause activity limitation. Common symptoms include tight muscles with decreased strength, abnormal gait, and lack of overall motor control and coordination. One recent development in the treatment of CP is the Therasuit method, which utilizes a dynamic proprioceptive orthosis that is worn by the patient during their rehabilitation sessions. The aim of this review is to assess the effectiveness of the use of the Therasuit in treating the symptoms of CP in children. METHOD: Several databases were searched using the terms cerebral palsy, treatment, and Therasuit. Seven articles were found and one was eliminated as it pertained to adults. The remaining six articles included a pilot study and case studies in addition to a randomized controlled trial, and all were used due to the paucity of the literature. PEDro scale was used to rate the articles. RESULTS: Articles scoring lower on the PEDro scale noted improvements in both GMFM and PEDI scores of the participants while the higher quality articles did not report any significant changes in these scores. CONCLUSIONS: Based on the minimal research that has been conducted, children who participated in the Therasuit method did not appear to show any improvements in their symptoms that were not also observed in children who participated in more traditional treatment protocols. Ashley Ortiz, Shingles: Diagnosis, Treatment, and Preventative. Mentor Denene Lofland: CHP I will be presenting a poster for the Medical Laboratory Science program. The poster will reflect information about shingles, herpes zoster virus, caused by the same virus that provokes chicken pox, varicella zoster, years later. It is a painful, blister skin rash that usually resides as a ring around the torso. Most people at risk for reactivation of the virus are immunocompromised or over 60 years old. There are other complications associated like vision loss, postherpetic neuralgia (PHN), and hospitalization if there are more than one immunocompromised conditions. The diagnosis can be done via polymerase chain reaction, which is the gold standard molecular technology for amplifying DNA for fastidious organisms in the lab. Treatments are primary antiviral prescription drugs, where also at the pharmacy the preventative vaccine can be given. Kelly OToole, Stem Cell Transplantation for Subjects with Duchenne Muscular Dystrophy. Mentor David Lake: CHP Purpose: This systematic review was done to determine if stem cell transplantation improves function in subjects with Duchenne Muscular Dystrophy (DMD). Hypothesis: It was hypothesized that cell transplantation would be a viable treatment. Methods: Databases used to find articles include PubMed, Medline, Cinahl and Pedro. Keywords and phrases used in the search include Duchenne Muscular Dystrophy, stem cell, transplantation, therapy, and treatment. Results: Twenty-eight articles were found to contain relevant information. After removing duplicates, Twenty-two articles remained, were screened and assessed. Fifteen articles did not fit the appropriate improve function criteria for this study. The seven articles remaining were used for this systematic review. One articles results showed an improvement in contraction force while another did not show significant improvement of contractility. One article showed no difference in muscle strength. Two articles showed some levels of donor dystrophin/dystrophin fibers at early testing, before reducing to non-significant amounts. Three articles did not have significant amounts of donor dystrophin/dystrophin fibers present. A major problem in comparing these articles was the differing definition of function in each study. Conclusion/Summary: Currently, stem cell transplantation cannot be considered as a therapeutic treatment to improve function and/or contractility in subjects with DMD. Based on the positive evidence, it is also suggested that with modifications and progression, stem cell transplantation could improve function in DMD subjects.
Quinn Pangborn, Joshua Hill and Austin Esch, Early Religious Involvement as a Predictor of Sensation Seeking and Feelings of Guilt and Shame. Mentor Vann Scott: CST It is undeniable that, as a whole, Western Culture demonstrates an interest in multiple facets of religiosity. Whether raised in a religious home environment or educated through personal interest, religious beliefs and environmental influences play key roles in how individuals perceive the integrity of their past and future behavior. An area of particular interest in its relationship to religiosity is that of sensation seeking, a term that is characteristically described as a trait revealed through varied, novel, complex sensations and experiences (Roberti2004). First measured by Marvin Zuckerman in 1994, sensation seeking includes behavioral modalities of physical, social, legal, and financial forms. Personal decisions can either motivate or hinder ones self-image based upon personal beliefs. The following study used self-report measures to determine levels of guilt and shame based upon hypothetical situations and sensation seeking variables in relation to religiosity. The hypothesis of this study supports the notion that individuals revealing a higher involvement in religiosity will experience increased feelings of potential guilt and shame in those proposed situations than do those with little religious involvement. A further correlation of interest will be scores of religious involvement to the measures of the sensation seeking component. Although this study is currently in its data collection phase, it is believed that the findings will fully support our hypothesis. James Parker, Question-writing as an active learning method. Mentor Nancy McCarley: CST An integral component of active learning in higher education is the design and implementation of techniques which can be used by students to retain classroom materials. Studies show that students who write multiple choice questions on textbook material will have higher exam scores than students who do not write questions. As most college instructors use the traditional lecture format, I asked if question-writing may be used to enhance retention of lecture information. I hypothesized that if students wrote multiple choice questions after a lecture they would score higher on a quiz relative to students who did not write questions. Participants were students who were enrolled in Introduction to Psychology (N = 103). Participants were randomly assigned to either the Basic Reflection or Question-writing group. All students attended a 30-minute lecture given by an instructor who was blind to the prupose of the study. Following the lecture, the Question-writing group wrote multiple choice questions based on their lecture notes and the Basic Reflection group simply studied their notes. Initial results indicated that there was no significant difference between the quiz performance of the Question-writing and Basic Reflection groups. Further analysis of the Question-writing group revealed a tendency for students to compose more specific (factual) questions and to perform better on the quiz if they composed more specific questions. As there was no impact of the Question-writing method on short term retention, a new phase of the study has been initiated to examine the long term impact of the method.
Kristina Pascutti, Adam Parker, Evan Boggs, and Melody Johnson, Identification of Fungi from Failed Sea Turtle Eggs. Mentor Jennifer Brofft Bailey: CST Sea turtles, including loggerheads (Caretta caretta), have long been a point of controversy as concern for their conservation continues to grow. Loggerheads are of particular interest to researchers in the southeastern United States because of the high number of nesting beaches found in Florida, Georgia and many other southern states. Previous research done by Sarmiento-Ramrez et al. 2010 showed that infection of loggerhead sea turtle eggs by the fungus Fusarium solani was responsible for a large number of failed eggs in nests on Boavista Island, Cape Verde. Non culture based PCR amplification of ITS regions were used to identify fungal contaminants in failed loggerhead sea turtle eggs collected from Jekyll Island, Georgia in 2012. Our long term research goal is to provide information that contributes to the continued protection of this highly treasured and valuable species. Hitixa Patel, Influenza. Mentor Denene Lofland: CHP Using my skills acquired in the medical technology program here I can help diagnose what organism and/or parasite exist such as bacterial or viral and combined with other factors as patients location, age, and other lab results I can help diagnose as to what it may be, rubella, measeles, and in my case influenza. Influenza is spread by respiratory droplets, coughing, sneezing. Many strains of influenza exist in todays world so how can we tell what strains we have? It may be strain A and type like H1N1 or H2N2, asian flu, bird flu, swine flu With Microbiology we can use minimal sample to diagnose it and combined with serogrouping with antibody we can narrow it down to specific subtypes. This virus has very defined structure consisting of glycoproteins: hemagglutinin and Neuraminidase which contain the viral RNA and genome activating the antibodies, according to which type of influenza virus. Nowadays in lab diagnosis we can easily and quickly find which type of viral infection the patient may have, and give accurate results with aid of RT-PCR, Viral culture, DFA antibody staining, Enzyme Immunoassay, etc. Due to the help of microbiology and rapid kits we can help reduce the rate of mortality in viral infections and help stop pandemics. Using proper hygiene, and in workplace using masks, and PPE, as well as taking vaccines and antiviral drugs if you are diagnosed with it. Kinnaree Patel and Shivam Patel, Site Saturation Mutagenesis of the Yeast Gene YDL124w at Mutation Site F125. Mentor Scott Mateer: CST Changing the stereoselectivity and stereospecificity of an enzyme can have an affect on how drugs are made and used in our bodies. YDL124w is a yeast gene belonging to the aldo- keto reductase family of enzymes. Mutating yeast gene YDL124w could cause a change in enzyme activity that perhaps can also change enzyme stereospecificity. As a class, we are trying to create a mutation at site F125 by changing amino acid phenylalanine (F) to other amino acid residues by site saturation mutagenesis via the method circular mutagenesis. My partner and me are going to change hydrophobic amino acid phenylalanine (F) to hydrophilic amino acids threonine (T) and asparagine (N) altering the chemical structure of the enzyme as well as its activity. We will determine if we have a mutation by doing a restriction digest using enzymes Pst1/Pvu1, where the enzyme Pst1 will be eliminated for positive clones because there would be no appearance of a cut in the DNA at the restriction site by this enzyme. The positive clones will then be sent off for DNA sequencing to verify if we have a mutation at the F125 site. Altering amino acid residues at mutation site F125 would help us further understand entioselectivity of enzymes that would create different enantiomers. Different enantiomers could be used to make different drugs that would heavily have an impact on how these drugs work in our bodies. Reema Patel and Mallory Rawson, The Roles of Leucine and Methionine in Determining YDL124wp Stereospecificity. Mentor Scott Mateer: CST YDL124wp is an aldo-keto reductase that catalyzes oxidation-reduction reactions. The mechanism involves reducing carbonyls to chiral alcohols, which connotes the stereospecificity of the protein. Enzyme stereoselectivity is a key component in developing new pharmaceutical drugs. The class project involves mutating the amino acid at position 125 in the gene YDL124w through site-saturation mutagenesis, from phenylalanine to every other amino acid. This experiment involves mutating that residue from phenylalanine to leucine or methionine, to determine if these specific amino acids will affect enzyme stereoselectivity. Phenylalanine is aromatic, larger and more hydrophobic than both methionine and leucine. Although these three amino acids differ in size, they all exhibit nonpolar physical properties that will not contribute to the enzyme stereoselectivity as the aromaticity of phenylalanine will. This mutation from an aromatic residue to one of the two non-aromatic residues will be the only factor affecting enzymatic activity. The desired mutation will be achieved through site-saturation mutagenesis, specifically circular mutagenesis. A double digest with Pst1 and Pvu1 will be used to screen for our desired mutation. When making the mutants, the Pst1 site will be eliminated, which will only allow Pvu1 to cut our mutated DNA. The final DNA will be verified by DNA sequencing. By focusing on mutating phenylalanine into leucine and methionine, it is possible to determine if either residue is involved in changing the stereoselectivity of the enzyme, which ultimately aids in the development of new drugs. Kaitlyn Patterson and Joshua Hill, Investigating Sex Differences in Gratitude. Mentor Wendy Wolfe: CST Gratitude has been found to reinforce social bonds, influence pro-social behavior, and decrease depression. Gratitude can be defined as a disposition (DG), a mood, or an isolated emotional experience. While gratitude is increasingly a topic of interest in the research literature, less focus has been directed to exploring sex differences in gratitude. Research involving DG found no sex differences (Watkins, Woodworth, Stone, and Kolts, 2003). However, a study by Kashdan, Mishra, Breen, and Froh (2009) found that women considered gratitude to be more beneficial and less costly than men did. Further, when receiving something from another, gratitude was a more of a motivating factor for males than females (Kolyesnikova, Dood, Wilcox, 2009). We sought to investigate sex differences in DG and in the effect of a benefit-triggered gratitude manipulation on experienced gratitude. Undergraduates completed an online survey that included two measures of DG (the GRAT and the GQ-6). Participants were then exposed to two timed tasks in which they were randomly assigned to a beneficiary condition (where they received assistance from a confederate), a benefactor condition (where they had the chance to provide assistance to a confederate), or a control condition. Participants were then evaluated on explicit feelings of gratitude by asking to list all of the things you feel grateful for and implicit gratitude through a lexical decision task using gratitude-related and neutral words. This research is exploratory and aims to further contribute to the literature on sex differences in gratitude. Kaitlyn Patterson, Olivia Singleton, and Joshua Hill Better to give or to receive?: The Role of Dispositional Gratitude Mentor Wendy Wolfe: CST Gratitude has been found to influence pro-social behavior, strengthen social bonds, and decrease depression. McCullough, Tsang, and Emmons (2004) described gratitude as existing at three interactive levels: dispositional gratitude (DG), gratitude as a mood, and gratitude as a discrete emotional experience. Their research also underscored the idea that event-based feelings of gratitude did not impact participants high in DG in the same way as those low in DG. In review of this finding, we speculated that individuals who tend to be grateful as a general state of being may feel more grateful after they have provided assistance, than after they have received assistance. This hypothesis was tested in our study. Thirty-three undergraduates (68% female, 65% Caucasian) were randomly assigned to a benefactor condition (where they were given opportunity to provide assistance to a confederate during a timed task), a beneficiary condition (where they received assistance from a confederate), or a control condition. We then assessed explicit and implicit feelings of gratitude. Our results illustrate a difference in how exchanges of assistance activate experienced gratitude among those with varying degrees of DG. Consistent with our hypotheses, those lower in DG feel grateful at an explicit level of awareness primarily when they receive assistance. At an implicit level, they seem to become less in touch with feelings of gratitude when they have provided assistance. Those higher in DG are more likely to have that feeling activated after they provide assistance, particularly on an implicit level of awareness. Nathan Peek, Synthesis and X-ray Structure Determination of Transition Metal Complexes Using Tripodal Imidazole Ligands. Mentor William Lynch: CST Our research has been focused on the synthesis and characterization of transition metal open face complexes using a tridentate ligand. The ligand, tris-1-ethyl- 4methyimidazolylphosphine is used because it creates a labile face which can be used to perform further experiments on. The complexes are prepared by the reaction of the ligand with metal precursors to form complexes which are analyzed by various spectroscopic and structural techniques. The synthesis of the ligand and several transition metal complexes will be presented as well as the complex characterization by spectroscopic and X-ray methods. Yolanda Perez, My Latina Identity: Marginalized & Homogenized. Mentor Rachel Green: CoLA My art deals with issues of Latina identity and culture, the exploitation of women of color and feminism ideals. I take on the absolute rejection of Latina stereotypes by acknowledging the great complexity that is over looked within the many different Hispanic cultures. My work is deliberately confrontational in order to expose some of the worst marginalized forgotten and ignored experiences with in the intricacy of our society. I incorporate traditional craft, various fiber arts techniques with multimedia aspects. Fiber art gives me a broad range of endless possibilities for personal expression and a sensuality that is universally nostalgic. Although there may not always be material or construction similarities between the pieces they are connected in theme and stylistic appearance. I emphasize intense colors, rich texture and expressive line quality to evoke drama. I am greatly influenced by the Nuyorican Diaspora art movement of the 1970s that revolved around identity, disillusionment, social struggles, and transcending boundaries. I am materialistically inspired by the many exotic cultures of the world and the skilled folk artisans within their societies. I bring elements of their craft into my work such as Indonesian batik, Chinese silk painting, Puerto Rican lace work, Caribbean hammock weaving and Oaxacan embroidery. I continue the attempt to blend the primitive kitsch elements of these cultures in to my work through the use of symbolism, pop culture references, iconography, and technique.
Taylor Peterman, Modeling the Feeding Biomechanics of the Southern Flounder (Paralichthys lethostigma). Mentor Austin Fancis: CST Feeding biomechanics is the study of how the muscles and lower jaws work together to allow fish to feed on prey. Biomechanics can be defined as the study of movement due to different forces. The forces causing movement in feeding are the contraction of the muscles attached to the lower jaws. The feeding biomechanics of fishes have been extensively studied for primarily bilaterally symmetrical species. Studies on bilaterally asymmetrical species are a topic that has not been explored to such degree. Fish that are bilaterally asymmetrical do not necessarily have the exact same head and mouth structure on each side, therefore when looking at the feeding biomechanics of these fish there needs to be measurements taken from both sides instead of just one primary side. These structural differences, even though they could be slight, could cause one side of the mouth to move or work differently than the other. This study examined the feeding biomechanics of the bilaterally asymmetrical southern flounder, Paralichthys lethostigma. Flounder are known to have both of their eyes on one side of their head, leaving one side to be the blind and the other to be considered the ocular. The interest of this study was to see what those differences, if any, were between each of the two sides. Twenty seven fish were dissected to reveal important morphological and biomechanical landmarks for feeding. The lower jaw and attached jaw closing muscle were excised and digitally photographed. Using image analysis software, important biomechanical measurements were made. Additionally, the weight of each division of the jaw closing muscle was determined. Courtnee Pettus and Melody Johnson, Change in Stereospecificity of YDL-124w through Site Saturation Mutagenesis. Mentor Scott Mateer: CST The purpose of this research is to understand the stereospecificity of the YDL-124w reductase enzyme and to determine if changing the amino acid at position 125 will result in a change in stereospecificity. Stereoisomers have a wide range of properties; being able to determine stereospecificity is a desirable ability when it comes to understanding the stereoselectivity of an enzyme. Dr. Mateers Spring 2013 Cell and Molecular Lab will be using circular mutagenesis to preform site saturation at position 125 to change phenyl alanine a bulky, nonpolar molecule to one of the remaining 20 amino acids. Cysteine and aspartic acid were the amino acids of interest in this research. Changing position 125 to cysteine or aspartic acid will result in a change in stereospecificity; however, cysteine will have a greater effect on function than aspartic acid because of its smaller size and polar nature. PstI/PvuI double restriction digest will be used to screen for positive clones and the elimination of the PstI cut site by the mutagenesis reaction will be used to identify putative mutations and the DNA will be sent off for verification. The ultimate goal of the class research is to determine if replacing the amino acid at position125 will result in a change in stereospecificity and if so which of the amino acids produces a desirable outcome, such as a more favored pharmaceutical drug. This will help to better understand how this particular stereoisomer carries out stereospecificity and if the stereospecificity can be changed. Magdala Pierre, Paramyxovirus. Mentor Denene Lofland: CHP Mumps is a paramyxovirus that causes an acute viral illness, distributed worldwide. An individual become infected by the virus through respiratory droplets, like saliva. Mumps first attack the nasopharynx and regional lymphs node then latter attacks different tissue such as the testes, ovaries, peripheral nerves, eyes, inner ears, pancreas, and central nervous system. The symptoms of mump include characteristic of parotitis which consist of painful swollen salivary gland. A vaccine is available to help reduce the number of cases of mumps virus; it was first made in 1967 and was not recommended for all children until 1977, when the Advisory Committee on Immunization Practices (ACIP) made a routine vaccination for all children of the age of 12 or older in 1977. Over the decades, the incidence of mumps decrease except an area where people were not following the laws such as getting one vaccine and not both, or not being vaccinated at all (CDC, 2012). The virus is diagnosed via serologic test such as enzyme linked immunosorbent assay (ELISA), direct and indirect fluorescent antibody (Lennette et al., 1985).
Inessa Pinto, Detection of Human Enteroviruses. Mentor Denene Lofland: CHP Enteroviruses include a diverse group of closely-related pathogens of the family Picornaviridae. Human enteroviruses are small nonenveloped single-stranded RNA viruses, including poliovirus, coxsackieviruses A and B, echovirus, and human enterovirus. They include over one hundred serotypes and usually cause self-limiting infections including mild upper respiratory infection, except enterovirus 71 and poliovirus, which may cause neurological complications. Outbreaks more typically occur in children under the age of five. Viral pathogens of this family may cause aseptic meningitis, encephalitis, febrile seizures, peripheral neuritis, and other CNS manifestations. Therefore, it is critical to use specific and sensitive tests aiding in diagnosis of human enteroviruses. Some of the traditional diagnostic methods are time consuming and labor dependent or simply ineffective. More sensitive and accurate tests come in place in diagnosis of human enteroviruses such as real time PCR and molecular tests targeting specific genes of the enteroviruses aiding in their identification. No vaccine is available for non-polio enteroviruses therefore personal hygiene and frequent hand washing may help in reducing the spread of these viruses. Nicolas Rios and SeungHun Lee, Ammonia-oxidizing Archaea Diversity with S. invicta presence. Mentor Jennifer Bailey: CST Solenopsis invicta, the red imported fire ant, is known for its painful sting, but also contributes to the soil ecosystems through aeration and enrichment processes. S. invicta excretes ammonia benefitting ammonia-oxidizing Archaea (AOA) utilizing ammonia as their energy source. AOA performs the rate limiting step of nitrification, a process that converts ammonia into nitrate. Some of the nitrate is leached from the system, potentially impacting plant productivity. A past study indicates AOA diversity increased with S. invicta presence. Our current project uses a mesocosm approach to ask how S. invicta mound development impacts AOA diversity and soil composition over time. Mesocosms are enclosures containing natural samples that are manipulated and monitored under controlled conditions. Two mesocosms were established in five gallon containers: treatment (ants) vs. control (no ants). Both mesocosms were established with non-mound soil from the same site. At time zero, both mesocosms were sampled before treatment. An active fire ant colony was transferred to the treatment mesocosm. Samples were then collected weekly at least weekly for one month. DNA was extracted and subjected to PCR amplification of the amoA gene (encodes for ammonia monooxygenase). The amoA gene is required by AOA and serves as a marker for this group. The PCR products will be cloned to sequence amoA genes present within the samples. The NCBI database will be used to compare sequences of known AOA, and determine AOA composition and diversity changes over the one month period.
Rebekah Robinson, Stem Cell Transplantation for Subjects with Duchenne Muscular Dystrophy. Mentor David Lake: CHP Purpose: This systematic review was done to determine if stem cell transplantation improves function in subjects with Duchenne Muscular Dystrophy (DMD). Hypothesis: It was hypothesized that cell transplantation would be a viable treatment. Methods: Databases used to find articles include PubMed, Medline, Cinahl and Pedro. Keywords and phrases used in the search include Duchenne Muscular Dystrophy, stem cell, transplantation, therapy, and treatment. Results: Twenty-eight articles were found to contain relevant information. After removing duplicates, Twenty-two articles remained, were screened and assessed. Fifteen articles did not fit the appropriate improve function criteria for this study. The seven articles remaining were used for this systematic review. One articles results showed an improvement in contraction force while another did not show significant improvement of contractility. One article showed no difference in muscle strength. Two articles showed some levels of donor dystrophin/dystrophin fibers at early testing, before reducing to non-significant amounts. Three articles did not have significant amounts of donor dystrophin/dystrophin fibers present. A major problem in comparing these articles was the differing definition of function in each study. Conclusion/Summary: Currently, stem cell transplantation cannot be considered as a therapeutic treatment to improve function and/or contractility in subjects with DMD. Based on the positive evidence, it is also suggested that with modifications and progression, stem cell transplantation could improve function in DMD subjects.
Madelyn Roush, Candace Poole, and Esmeralda Rivas-Torres, Investigating the reductases YHR104w and YOR120w. Mentor Scott Mateer: CST Chiral alcohols are highly desirable pharmaceutical building blocks, but their synthesis from carbonyl compounds by chemical and industrial methods can be hazardous, difficult, and costly. One potential strategy to safe, easy, and cost-effective synthesis of these chiral alcohols is the reduction of keto-esters by aldo-keto reductases (AKRs). However, it is often laborious and time consuming identifying the ester/enzyme combination that produces the desired stereochemistry required for a particular medical compound. Our work seeks to explore the stereoselectivity of YHR104w and YOR120w by identifying amino acid residues that are important for substrate binding and orientation. For our analysis we have targeted residues located in the reductases Substrate Specificity Loop A (Loop A). The AKR YOR120w primarily produces one diasteriomeric product when reducing -Chloro--keto esters while the AKR YHR104w produces a different diasteriomeric product. Mutagenesis of the residues of Loop A on YOR120w and YHR104w was conducted because the residues of the Loop A region of these aldo-keto reductases dictates the stereoselectivity of the enzymes. The Loop A region of YHR104w was substituted for the Loop A region in YOR120w, and the Loop A region of YOR120w was substituted for the Loop A region in YHR104w to determine if opposite chirality is observed in the reduced products of keto-ester substrates. The wildtype and mutant proteins were expressed in bacteria as GST-chimeras, and the resulting crude lysates were used to characterize the reduction of several keto-ester substrates. Jamie Rowell, The diaphoretic effect of exercise: Determination of sweating threshold and sensitivity. Mentor Lorrie Hoffman: CST During exercise, there occurs a point at which we begin to sweat. In a study conducted on sixteen subjects exercising on a stationary bicycle, the esophageal temperatures and sweat data were collected. In a data set comprised of time since the start of exercise associated with a measure of sweat collected, the linear regression system follows a typical broken-line model. This means that there occurs a period of little or no activity, then a linearly increasing measurement of sweat, followed by a dip (the point at which the subject cannot sweat anymore), ending with a plateau. In the research conducted on this experiment, we are interested in what we refer to as the first change point. This change point is the point at which the data switches from a period of little or no activity to a linearly increasing measurement of sweat; it is this point where the subject begins to sweat. In order to determine if the occurrence of a change point exists, Quandt (1958) first suggested using a likelihood ratio test, which expresses how many times more likely that within the data, a change point occurs than the alternative that a change point does not exist. Using Quandts method of estimating the parameters of a linear regression system following a broken-line model, as well as his use of the likelihood ratio test in determination of the existence of a change point, we examine the point at which sweating begins for each subject, and their relationship to each other.
Michalle Ruble, A Kindergarten Case Study: An In-Depth Analysis of a Phonemic Awareness Assessment. Mentor Anne Katz: CoE The purpose of my case study research was to analyze the results of a Phonemic Awareness Assessment administered to a kindergarten student who is beginning to learn how to read and to apply this knowledge to my future pedagogy. As a teacher candidate, I need to determine age-appropriate phonemic awareness competencies in the early elementary grades in order to develop reading instruction that supports student success. Success in reading occurs when students are skilled in phonemic awareness, among other constructs. This idea is supported by the National Reading Panel (2000), who concluded that phonemic awareness instruction improves reading skills in students. The Phonemic Awareness Assessment for this case study included the following elements: isolation of sounds, deletion of sounds, segmentation and blending of phonemes, letter name recognition, and phoneme manipulation (including single consonants, consonant blends, vowel sounds, digraphs, phonograms, and r- controlled vowels). For the purpose of my poster presentation, I will critically analyze a kindergarten student's performance on the initial consonant blends portion of the assessment via various modalities. The subject of the case study displayed greater proficiency in letter-to- sound than sound-to-letter consonant blend identification. Evaluating and reflecting upon the student's assessment data will provide insight into the acquisition of phonemic awareness skills for young readers. An analysis of results affords a valuable opportunity for future teachers to acquire assessment skills and insight in regards to phonemic awareness acquisition for early readers. Rebekah Sapp, Tall Ships and Green Beer. Mentor Michael Toma: CoLA This study is a time-series analysis measuring the impact of Savannah's local Tall Ships festival and St. Patrick's day festival on the hotels and motels in Savannah. Impact studies are common because the information is so important to those that plan the events, and events are often planned based on the assumption that they will boost economic activity. Contrary to popular belief, events usually do not have the impact they are expected to bring. Much of the literature on the topic finds that events bring far less money into the economy; some even find a negative impact on regional economies. This impact study differs in that it measures the impact not on the regional economy as a whole, but on the local hotels and motels. The festivals were tested as determinants for local hotel's and motel's occupancy rates, average daily rates, and revenue per room. A total of 27 regressions were run with dependent variables accounting for one of these three rates, and either the location, size, or ranking of the hotels and motels, based on the prices. Controls for the state of the economy, controls for the hotel and motel rates of the entire state of Georgia, and dummy variables accounting for the month in which the events took place and also different characteristics of the events were all included in the regressions. The model was tested for some typical issues with time-series analyses and was corrected for statistical errors to make the model workable and valid, which gave our impact for the local hotel sector. Ashley Schwab, Muscle Activity in Overhead Athletes with Shoulder Impingement Symptoms: A Systematic Review. Mentor David Lake: CHP INTRODUCTION/PURPOSE: Shoulder impingement is one of the most common causes of shoulder pain found in overhead athletes. It is usually due to weaknesses found in the surrounding musculature and a subsequent unstable glenohumeral joint. The aim of this review is to determine the activity of muscles used in the shoulder of overhead athletes with signs of shoulder impingement. METHODS: Several databases were searched using the terms shoulder impingement and overhead athletes. RESULTS: Ten relevant articles were found. Three of these were review articles and were eliminated. One was eliminated because it was exactly the same study included in another article. The six remaining articles included in this review are repeated measures, quasi-experimental, non-experimental. Basic findings throughout the articles were signs of decreased force production and muscular weakness of the serratus anterior. In addition, other findings included decreased activity in the lower trapezius. Each of the previous findings have been shown to contribute to glenohumeral instability and joint laxity. In a separate study, it was also found that a six-week scapular rehabilitation program resulted in improved pain, function and muscular force output and activation in symptomatic shoulders. CONCLUSIONS: Based on the research that has been conducted, overhead athletes with symptoms of shoulder impingement showed decrease forces and imbalances within muscle groups around the shoulder.
Mrinali Sharma and Jaleesa Mcqueen, A Base-promoted Alcohol Dehydration Ru(II) Catalyst: Reaction and Scope. Mentor Brandon Quillian: CST (BPA)RuBr(PPh 3 ) 2 (BPA =bis(pyrazolyl)acetate, Ph= phenyl) serves as an alcohol dehydration catalyst under basic conditions. It was found that (BPA)RuBr(PPh 3 ) 2 , in the presence of potassium hydroxide, silver trifluoromethanesulfonate and dodecene, dehydrates (R,S)2-phenylethanol and 3-phenylpropanol at 150C over several hours to yield their respective alkenes (confirmed by GC-MS, IR, and NMR). Though dehydration is commonly facilitated by converting the alcohol into a better leaving group, it often requires reaction with strong acids or highly toxic and reactive phosphoryl chloride. Further elucidation of the reaction properties, could transform this novel reaction into safer method to convert alcohols into alkenes. We describe herein our current understanding of the properties of this reaction and future plans. **Caroline Shem-Tov, Does treatment for depression reduce likelihood of developing dementia? Mentor April Garrity: CHP There is a common correlation between depression and dementia that is poorly understood by allied health professions. Knowing the different types of depression, their neurological pathologies, and their possible effects can result in early detection, prevention, and improvement of dementia. This research is a compilation of six peer reviewed meta-analyses of neurological studies on depression, dementia, and brain damage located on Ebsco Host. The types of depression reviewed are anhadonia (apathy), dysthemia, and broad depression. The different forms of dementia considered are insidious regressive dementia, dementia from neurological lesions, neurovascular dementia, and dementia resulting from chemical inflammation within the brain. Studies of patients who have unspecified depression resulting from lesions caused by a cardiovascular attack and other forms of brain damage report improved cognition when taking anti-depressants. Additionally, treatment of dysthemia and general depression can reduce the development of dementia caused by chemical inflammation resulting from depression. Anti-depressants have no effect on depression and dementia resulting from insidious regressive neurological pathologies such as Alzheimer's disease, however, early treatment can impede the rate of decline. By knowing the different types of depression, medical professionals can more appropriately refer patients to the proper medical provider such as a neurologist or a psychiatrist in order to properly treat the depression. The treatment of dysthemia and broad depression will result in a better long-term dementia prognosis, whereas the identification of anhadonia can be an indicator of brain damage from an insidious regressive neurological pathology, which early detection and treatment can decelerate the progression of the disease. Marina Simonet, Heather Hullum, Aaron West, and Marlayna Garvin, Affects of Seating Arrangement on Personal. Mentor Angie Koban: CST Typical classrooms use seating arrangements which do not promote eye contact or interaction between students, two factors that have been proven to increase student success. This study examines the impact of different seating arrangements on communication. Numerous studies have shown that seating arrangement affects students behavior, interaction, and participation at all levels of schooling. Specifically, circular seating arrangements increase active participation which is conducive to academic success. Here we are examining the effect of seating arrangement on willingness to disclose personal information. We theorize that those students arranged in a circle seating arrangement will be more willing to disclose personal information than those in row-and-column and back-to-back. This data will help professors and administrators at the university level to create the optimal classroom environments in which students feel comfortable among peers and professors. Olivia Singleton, Kaitlyn Patterson, and Joshua Hill, Better to give or to receive?: The Role of Dispositional Gratitude. Mentor Wendy Wolfe: CST Gratitude has been found to influence pro-social behavior, strengthen social bonds, and decrease depression. McCullough, Tsang, and Emmons (2004) described gratitude as existing at three interactive levels: dispositional gratitude (DG), gratitude as a mood, and gratitude as a discrete emotional experience. Their research also underscored the idea that event-based feelings of gratitude did not impact participants high in DG in the same way as those low in DG. In review of this finding, we speculated that individuals who tend to be grateful as a general state of being may feel more grateful after they have provided assistance, than after they have received assistance. This hypothesis was tested in our study. Thirty-three undergraduates (68% female, 65% Caucasian) were randomly assigned to a benefactor condition (where they were given opportunity to provide assistance to a confederate during a timed task), a beneficiary condition (where they received assistance from a confederate), or a control condition. We then assessed explicit and implicit feelings of gratitude. Our results illustrate a difference in how exchanges of assistance activate experienced gratitude among those with varying degrees of DG. Consistent with our hypotheses, those lower in DG feel grateful at an explicit level of awareness primarily when they receive assistance. At an implicit level, they seem to become less in touch with feelings of gratitude when they have provided assistance. Those higher in DG are more likely to have that feeling activated after they provide assistance, particularly on an implicit level of awareness. **Caitlin Solomon and Leslie Childress, Treatment Options for Individuals with RHD. Mentor April Garrity: CHP Our research seeks to answer the question: Should speech language pathologists treat individuals with right hemisphere damage with treatment that focuses on the clients deficits or a functional approach to treatment? Right Hemisphere Damage is damage to the right side of the brain, which can lead to cognitive-communication problems, such as poor reasoning, attention problems and impaired judgment or attention (ASHA, 2013). A thorough literature search yielded four articles which reviewed the treatment options for communication deficits associated with right hemisphere damage: Mackenzie, et al (2008); Blake (2007); Tompkins (2012); Blake (2013). All of the findings conclude there is not enough evidence to state whether a certain treatment is effective or not, however two articles state that a clinician may treat a patient based on their deficits. They emphasize the importance of using similar treatment methods for treating traumatic brain injury since the two injuries are closely related, and encourage looking at what the client cannot do and treating each deficit individually. The latter two articles encourage clinicians to treat with a functional approach focusing on what the individual with right hemisphere damage can do rather than what they cannot do. Although research for treatment of right hemisphere damage is in its preliminary stages, we concluded that clinicians could approach therapy in two different ways. They can target therapy with a functional approach improving the areas of communication that the client has preserved, or they can target the clients deficits using similar treatments used for individuals with traumatic brain injury. Brittany Speller, Exploitative Medical Science and Race. Mentor Mark Finlay: CoLA This project traces the parallels between the treatment of African Americans through the Tuskegee Syphilis experiment, and those of HIV/AIDS studies centered on minorities. Despite the existing regulations and ethical standards of health professions that have emerged since the Tuskegee study ended in 1972, African Americans are still marginalized and exploited by the medical field. Although African Americans have made undeniable gains made, evidence of racism can still be found in HIV/AIDS research studies and pharmaceutical testing of HIV/AIDS medications. The goal of this project is to illustrate corresponding practices of the Tuskegee study and HIV/AIDS studies involving African Americans by comparing the procedures, historical context, witness statements, and medical ethics contexts of each study. This type of information is imperative to African American history, and to understanding the complex relationship between African Americans and the medical field. Shyamkumar Sreekumar, Coding in Objective-C to Generate Chemistry Applications. Mentor Clifford Padgett: CST Objective-C is the primary programming language that was used to write software for the iOS Operating system. Objective-C inherits the syntax, primitive types, and flow control statements of C and includes syntax for defining classes and methods. The compiler implemented was X-Code, an integrated development environment (IDE) designed for developing iOS and Mac apps. It also includes editors used to design and implement apps, such as a source code editor and a user interface editor. These were some prerequisites necessary to code programs on the iOS SDK Interface. The purpose of this research project is to create fundamental applications of chemistry exclusively for the iPad market using an object- oriented computer language. The primary objective is an iPad application that one can utilize in practicing chemistry concepts such as chemical reactions, atomic structures, stoichiometry, etc. Using Beginning iOS 5 Application Development by Wei-Meng Lee, step-by-step instructions were followed to code preliminary projects such as Hello World, random number generators, and image processing. These were compiled and simulated using the X-Code IDE along with debugging for syntax errors. Although currently, research advancement is in the preliminary stages of development, I perceive that through extensive use of this coding compiler further development of chemistry applications will be successful. Teresa Stewart, DuBois's View on Double Consciousness. Mentor Mary Barr: CoLA I recently had the opportunity to attend a celebration of the life and works of W.E.B. DuBois at Clark Atlanta University. This included four days of step shows,descendants of historical black figures, a jazz show, and several panels of educators and community leaders discussing DuBois's works and views. During a few of the panels they discussed DuBois's view on Double ConsciousnessWhich is a concept that DuBois first explored in 1903 in the Souls of Black Folks. It describes the individual sensation of feeling as though your identity is divided into several parts, making it difficult to have just one identity. DuBois spoke of this within the context of race relations in the United States, that since American blacks have lived in a society that has historically repressed and devalued them that it has become impossible for them to unify their black identity with their American identity. Double consciousness forces blacks to not only view themselves from their own unique perspective, but to also view themselves as they might be perceived by the outside world. This is what DuBois meant when he talked about The sense of looking at one's self through the eyes of others. This really intrigued me as I feel like this is a way of everyday life for me, living and working in two completely different worlds. Matthew Trivitayakhun, Examination of 4-(aminomethyl)phenyl)methanol (APM) as a Photo- liable Linker. Mentor Brandon Quillian: CST We are evaluating 4-(aminomethyl)phenyl)- methanol (APM) as a photo-labile linker to connect an anti-cancer drug to polymer coated nanorods for the controlled release of the drug under UV radiation. ortho-Nitrobenzyl derivatives (ONBD) are commonly used as UV-labile linkers in such biological applications, however, the nitroso by-product formed upon decay is toxic to proteins. We selected 4-aminomethylbenzyl alcohol as a substitute for ONB in this study to avoid the formation of harmful by-products. We have prepared a complex with fluorenylmethyloxycarbonyl (FMOC) connected to the alcohol and amine groups of linker to form a drug-linker-nanorod mimic. This complex exhibits enhanced decay under UVC radiation, which suggests APM may be viable linker in our ultimate study of the drug-linker-nanorod conjugate. Herein, we discuss our syntheses, properties and UV-radiation decay of mono- and di-FMOC substituted AMP compounds. Madalynn Walker, M. Lamb, and S. Schwartz, Characterization of Bacteria Present in Failed Log Loggerhead Sea Turtle (Caretta caretta) Egg. Mentor Jennifer Bailey: CST Loggerhead hatch success is significantly lower in Georgia than for sea turtles globally. Microbial infection represents one explanation for why eggs fail to hatch. In 2010, failed loggerhead eggs were collected along the coast of Jekyll Island, GA. Thirty failed eggs from 6 nests were analyzed by 16S rDNA clone library analysis to determine the types of Bacteria they contained. Thirty bacterial groups were detected based on 395 analyzed sequences. Nine groups were recovered from multiple nests whereas others were only seen in either a single egg or nest. Seven of these groups were 99% identical to 16S sequences of known or suspected pathogens, including members of Vibrio, Enterobacter, Pseudomonas, Stenotrophomonas, Hahella, Achromobacter and Klebsiella. The detection of Hahella chejuensis is of interest since it produces a prodigiosin (red pigment) which inhibits the growth of certain cell types and has immunosuppressive activity. Failed loggerhead eggs often contain pink-red pigmentation, but the cause has not been determined. PCR primers specific to H. chejuensis were used to screen 40 DNA samples extracted from failed egg contents. H. chejuensis PCR products were produced from all eggs containing a pink-red biofilm and/or fluid. A faint product was obtained from two eggs containing no pigmentation; all other eggs tested negative with the screen. Primer specificity was confirmed by sequence analysis of the PCR products. Additionally, we have cultivated H. chejuensis isolates from red-containing failed loggerhead eggs from Jekyll Island during 2012. Collectively, the data suggests that multiple bacteria may impact loggerhead eggs on Jekyll Island. Also, we have demonstrated that H. chejuensis has been associated with failed eggs over nonconsecutive nesting seasons and may be responsible for their frequent red pigmentation. Further work is needed to determine if H. chejuensis is pathogenic to loggerhead embryos. Blair Weaver, Asymmetric Synthesis of Chiral Lactones. Mentor Brent Feske: CST Chiral lactones are used in a variety of pharmaceuticals and commonly found as many insect pheromones. We have demonstrated the synthesis of -ketonitriles with aliphatic aldehydes as our starting material through the use of umpolung chemistry. These -ketonitriles were then screened for their ability to be reduced by a bakers yeast ketoreductase library, and the enzyme that had the best ee was used for the reaction scale up to the chiral alcohol. The resulting enzymatic product is hydrolyzed and spontaneously cyclized to yield the chiral lactone. With the hexanal substrate, we have demonstrated the ability to yield the chiral alcohol using a bakers yeast reductase library and have made the chiral lactone. With biocatalysis as the key step, the synthesis of a variety of chiral lactones has been shown.
**Veronique Webber and Alex Hinton, Does Neurologic Music Therapy Improve Aphasia? Mentor April Garrity: CHP Aphasia is a speech and language disorder resulting from dysfunction in a specific region of the brain. The discipline of music therapy seeks to use music within a therapeutic relationship to address the social, emotional, physical, and even neurological needs of an individual.The purpose of our research is to determine if expressive language improves more using neurologic music therapy than it does with traditional non-intonation-based speech therapies for individuals with aphasia. For this project, terms from the research question were used to search for appropriate peer-reviewed research journals. This literature search yielded four appropriate articles for review. Following our detailed search, a systematic review of each journal article was conducted. The studies reviewed show measurable recovery while using music therapy for treating neurological language and speech disorders. The clinical expertise and opinion of a locally certified music therapist was also considered while attempting to answer our research question. We were given the opportunity to observe music therapy sessions and conduct interviews with involved individuals to gain client and caregiver perspectives. In addition to improving verbal expressions, neurologic music therapy was also found to improve personal motivation and emotional states of patients. According to Wan, et al. (2010), a majority of the research has shown that singing the intonation of spoken words and phrases can help facilitate expressive language beyond the limitations of either natural recovery or traditional non- intonation-based speech therapies in patients with aphasia (p. 291). Allison Williams and Serina Doolittle, Site Saturation Mutagenesis of YDL124w. Mentor Scott Mateer: CST We are interested in using enzymes to do important chemistry in order to make medicines more efficiently. We are particularly interested in the asymmetric synthesis of chiral alcohols since they are important precursor molecules for several pharmaceutical compounds. We have been using the yeast reductase, YDL124w, as a model enzyme to understand how enzymes select for one enantiomer over another. YDL124w has been shown to reduce keto- esters into chiral alcohols, and does so in enantioselective manner. Previous research identified a region in Loop A that was important for substrate binding. Specifically simultaneous mutation of residues 123 125 (SPF) to Alanines resulted in a mutant enzyme that had a significantly lower ability to reduce keto-ester substrates. To investigate this result further, we generated three additional mutants in which the individual residues were changed to Alanines. To verify that the correct mutants were made, mutant DNA was harvested and putative clones were identified by Pst I/Pvu I restriction digest. The selected positive clones were then verified by DNA sequencing. Further research will be conducted to determine more about the role that residues 123-125 play in determining enzyme enantioselectivity.