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=
This number in conjunction with Impeller Rotational Speed (N),
Impeller Diameter (D) and Liquid Density () allow us to calculate the
Mechanical Power (P) being transmitted to the fluid by a
turbine/impeller of a given design.
Reynolds Number is the second key number in the set of
dimensionless numbers. Again similar to applications in pipes, etc.,
the Reynolds number indicates the degree of turbulence experienced
in a stirred tank reactor.
2
Re
ND
N =
Where is the viscosity of the liquid in which the agitator is turning.
Flow Number (NQ) Useful measure of the pumping capacity of an
impeller. Again the number is design specific and independent of
scale.
3
ND
Q
N
Q
=
Aeration Number (NQg) Useful measure of the gas dispersion
capabilities of the impeller.
3
ND
Q
N
g
Q
g
=
P = agitator power (W) (N.B. Shaft power only)
D = impeller diameter (m)
= fluid density (kg m
-3
)
N = impeller speed (s
-1
)
= fluid viscosity (Ns m
-2
)
Q = fluid flow rate (m
3
s
-1
)
Qg = gas flow rate (m
3
s
-1
)
The Relationship of Power Number and Reynolds Number
Relationship has three phases each phase corresponding to the
three phases of liquid flow, laminar, transition and turbulent
A plot of Ln NP vs Ln NRe straight line, slope 1
Turbulent flow, Np independent of NRe (also constant)
Bioreactors are, in the main, in turbulent flow. This means that the
power number is constant for a given impeller design. Power
numbers for a variety of impellers in turbulent flow have been well
characterised, therefore if we know the impeller diameter and the
rotational speed of the impeller (both easy to measure) we can
subsequently estimate the mechanical power input to the bioreactor.
It is important to note that all of the correlations presented apply to
ungassed, single phase fluids only no allowances for aeration or
suspensions.
In general the Gassed Power is less than the calculated ungassed
power. A general rule of thumb for the calculation of gassed power is
Pg = 0.6 P
Example
Calculate the specific power requirement (P/V) for a standard
configuration STR, fully baffled, fitted with a Rushton turbine and
containing water at 25
0
C. The vessel diameter is 0.5m. The impeller
speed is 300rpm.
Solution
Standard STR T = 0.5m
D = T/3 = 0.167m
H = T = 0.5m
V = [ T
3
/4 = 0.098m
3
( )
3
2
2
Re
10 1
167 . 0
60
300
1000
|
.
|
\
|
= =
x
ND
N
5
Re
10 4 . 1 139445 x N ~ =
fully turbulent flow, therefore from the Power Number Reynolds
Number correlation graph, (curve 1 is a Rushton turbine remember
not to misread the log scale!)
NP=5
P=NpN
3
D
5
= (5)(1000)(300/60)
3
(0.167)
5
= 81W
Power input per unit volume is a useful comparitive measure between
bioreactors of different scales
3 3
/ 1 / 828
098 . 0
81
m kW m W
V
P
~ = =
Typical Specific Power Consumptions (P/V) kW/m
3
Mild agitation 0.1
Suspending light solids
Blending of low viscosity liquids
Moderate Agitation 0.4
Gas dispersion, liquid-liquid contacting
Some heat transfer
Intense Agitation 1.0
Suspending heavy solids, emulsification
Blending pastes, dough 4.0
Industrial-scale fermenters 0.5-5
Lab-Scale fermenters 5-10
Reynolds Number ranges for Rushton turbine
Re < 10
1
laminar flow
10
1
< Re < 10
4
transitional flow
Re < 10
4
turbulent flow
Mixing Effectiveness
- Mixing time tm time required to achieve specified degree of
homogeneity, starting from the completely segregated state
- A subjective quantity
- Measured by tracer studies
Inject a tracer pulse into the agitated vessel
Monitor concentration at a single point
- Colouring/decolouring method
- e.g. methylene blue, iodine/starch
- simple to implement
- monitor by eye/spectrophotometer
- good for detection of stagnant regions
but - dye may adhere to biomass
- Coloration is irreversible (disposal?)
- vessels seldom transparent sampling
- conductivity
- electrolyte tracer e.g. KCL added to vessel
- monitor response using conductivity probe
- fast probe response time
- cheap and reliable for small scale systems using water
But - bubbles interfere with measurement
- addition of electrolyte to broth changes in osmotic
pressure rheological effects
- not suitable for actual fermentation systems
- pH
- acid added
- one (or more) pH probes to monitor response
- pH probes sterilizable, widely available
- acid addition circuit available for pH control
- most suitable for large-scale applications
- suitable for three-phase systems
but - pH signal requires careful interpretation
Correlations for tm in Stirred Tank Reactors
Single-phase liquids
For fully turbulent flow, the energy delivered to the fluid by the
impeller P, is completely transformed into kinetic energy of the liquid:
2
2
5 3
u
Q D N N P
P P
= = (1)
Where QP is the pumping capacity of the impeller (m
3
s
-1
) and u is the
liquid velocity as it leaves the impeller. For an impeller blade width w,
Dw u Q
P
H = (2)
The circulation time tcirc is defined as
circ
circ
circ
Q
V
t = (3)
For an agitated vessel, Qcirc, the circulation capacity is greater than
the pumping capacity QP due to liquid entrainment by the impeller.
Experimentally it has been determined that:
P circ
Q Q 2 ~ (4)
The mixing time tmix is related to tcirc as follows:
circ mix
t t 4 ~ (5)
Assuming Vcirc = V = HT
2
H/4 and that
2
2
u u = (6)
Equations (1)-(5) yield
33 . 0
2
3
(
(
|
.
|
\
|
|
.
|
\
|
|
.
|
\
|
=
D
w
N
T
H
D
T
N
c
t
P
mix
(7)
For the assumptions made above c~0.6.
From equation (7), for fully turbulent flow (i.e NP constant)
Ntmix = constant (8)
For H=T and w=0.2D,
( )
| |
33 . 0
3
/ '
P
mix
N
D T
N
c
t = (9)
Where c
mix mix
t t 2
2 ,
~ for equation (9), c
~ 6.
Significance of tmix for bioreactor operation?
- PH measurement and control?
- DO concentration?