Documentos de Académico
Documentos de Profesional
Documentos de Cultura
culture media
Contents
4 2 Committed to Meeting Your Challenges 15 2 Product Insight Range and Culture Media
TM
44 2 Automated Specimen Inoculation - Previ Isola 46 2 Specimen Protocols Guidelines From Specimen to Plate
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Laboratories are more than ever a pivotal role in ensuring patients receive the most appropriate healthcare rapidly given the current worldwide context.
Hospitals are faced with healthcare-associated infections that are recognized worldwide as a major public health issue. They need effective microbiology solutions to help with the prevention, intervention and surveillance in the fight against HealthcareAssociated Infections (HAI). Management of healthcare expenditure implies having the means to ensure improved patient triage, better discerned use of antibiotics, freeing up patient beds quicker etc Regulatory requirements are ever present and form an integral part of patient safety. Time to result is increasingly important for many diseases; this means having a linear workflow right from sample management and colony isolation to result print out.
As a key part of this healthcare picture, you as a laboratory need to have confidence in the microbiology solutions you chose to address these daily challenges. This is why we are committed to offering you an extensive range of cost-effective, nutrient pre-plated media. They are technically sophisticated to help ensure you obtain easy-to-read, rapid results, whatever the quantity and type of microorganism in the specimen.
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bioMrieux is still committed to offering timely, relevant solutions now and in the future. Today, with bioMrieux, obtaining quality media solutions is simpler than ever before. 5 G GGG G
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USA PORTLAND
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AUSTRALIA BRISBANE
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To facilitate your regulatory requirements, we also provide a complete range of documents on-line in our Technical Library: www.biomerieux.com Package Inserts Non-hazardous Product Statement Declaration of Product Conformity Statement Thermal Shock Statement Quality Control Certificates
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WHAT D O E S B I O M R I E U X P R OV I D E TH E U S E R A S P R O O F O F P R O D U C T Q UALIT Y ? PA C K A G E I N S E R T S
WHAT D O E S IT COVE R ?
QUALIT Y CONTROL C E R T I F I C AT E S
The Quality Control Certificate is available for each Lot: bioMrieux provides performance criteria and
the collection strains used for Quality Control of culture media in the certificate.
NF EN 12322 "Culture media for microbiology / Performance criteria for culture media" (July 1999)
This standard concerns performance of culture media used in microbiology labs (traceability, compatibility, reproducibility of product performance throughout the products life cycle and suitability.
NF EN 375 "Information provided by the manufacturer with in vitro diagnostic reagents for professional use" (April 2001)
This standard concerns information supplied by the manufacturer of in vitro diagnostic reagents including reagents, calibrators, control material and kits. It defines the information which must be indicated on labels and instructions for use of each IVD product.
NF EN 1041 "Information supplied by the manufacturer with medical devices" (October 2008)
This standard concerns the information to be provided by the manufacturer for different types of medical devices covered by the EU Directives.
Clear and Easy Symbols for all Users: given on each product's label and package insert. Symbols reduce the need for multiple translation in local languages, simplify labeling, prevent the creation of different symbols conveying the same information.
NF EN 980 "Graphical symbols for use in the labelling of medical devices" (July 2008)
This standard concerns the graphical symbols to be used on information provided by the manufacturer for medical devices.
ISO 15223 "Symbols to be used with medical device labels, labeling, and information to be supplied + amendments" (April 2000)
This standard concerns symbols conventionally used to convey information essential for the correct and effective use, safe and effective use of medical devices.
PA C K A G I N G
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Environmental Protection
In addition to following the European directive 94/62/EC Packaging and packaging waste, one of bioMrieuxs guiding Principles is "We belong to a community". It is only natural that we embrace a responsible attitude toward our environment in all aspects of our business worldwide as regards energy, water, paper, waste, emissions.
As part of our environmental actions for our media products, we still use recycled and recyclable cardboard to make our boxes but we have worked even further on reducing the volume of plated media packaging. By moving from our already innovative fold-boxprinciple to an ingenious, new, patented fold-lockTM principle (once flattened the box stays flat) we further facilitate the disposal of pre-plated culture media boxes and reduce your daily waste volume and weight. Not only do we actively try to contribute to saving our environment but we help you to do the same.
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25C
15C
Storage temperature
Shelf-life
2 Example of a thermal shock protocol for preplated media: Storage: 2-8C 10-15C for 4 weeks 18-25C for 6 days, including 35-39C for 8 hours 15-25C for 4 weeks 2-8C
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Customer Service
Our customers deserve only the very best and as such bioMrieux is committed to continuously improving the quality of products and services we deliver you.
Our Customer Service meets this challenge by offering a wide range of services: rapid responses to customers' questions and complaints, tailor-made customer product training courses, proactive and curative system maintenance and even workflow auditing. To ensure you receive first class service regardless of your geographical location, local bioMrieux technical assistants specialized in microbiology are ready to help you backed up by the expertise of the global customer service support team. This organization provides a winning combination of local proximity with worldwide, up-to-date knowledge to ensure our customers have the expertise and follow-up they require when needed.
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Product Insight
17 2 23 2
Chromogenic Media for Simultaneous Isolation and Immediate Identification Chromogenic Media for the Screening of Multidrug Resistant Organisms
25 2 Blood Agar: Complex, Selective and Differential Media 29 2 Specific Media: Selective Isolation for Specific Organisms 38 2 Simple Media: Isolation of Organisms 41 2 Media for Susceptibility Testing 42 2 Media for Environmental Controls: Surfaces, Air, Water
For more details see technical sheets
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An extensive range of chromogenic media for the simultaneous culture and identification of microorganisms
With chromIDTM bioMrieux offers you an extensive range of chromogenic media for the simultaneous culture and identification of microorganisms. Designed to meet your increasing daily challenges, these high quality media provide you with reliable results and true peace-of-mind. Our recognised expertise in culture, identification and susceptiblity testing naturally led us to pioneer the chromogenic media concept back in 1989. Since then, we have continued to create innovative chromogenic solutions to meet your everyday needs.
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2CHROMOGENIC
TM
M E D I A F O R S I M U LTA N E O U S I S O L AT I O N A N D I M M E D I AT E I D E N T I F I C AT I O N
chromID CPS
chromID CPS
Incubation: 24 hours E.coli ATCC 25922, Proteus mirabilis ATCC 43071 and Enterococci ATCC 29212
Chromogenic medium for the enumeration of organisms in urine specimens and the direct identification of Escherichia coli, Enterococcus, KESC and Proteeae. Selective isolation of fastidious bacteria. Determination of hemolysis.
* Consult your local bioMrieux representative for more product information and availability.
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M E D I A F O R S I M U LTA N E O U S I S O L AT I O N A N D I M M E D I AT E I D E N T I F I C AT I O N
chromID Candida
TM
of Candida albicans
C. albicans
C. krusei
chromID Candida
Ref. Ref.
chromID Salmonella
Incubation: 24 hours S. Typhimurium ATCC 14028
chromID Salmonella
2 for the selective isolation of all Salmonella serotypes
Three chromogenic substrates optimize the selective isolation and differentiation of Salmonella: Specific detection of the esterase enzymatic activity on a colourless background = Pale-pink to mauve colonies for Salmonella (reading between 18 to 24 hrs) Differentiation of other bacteria (colonies of a different colour) Inhibition of Gram-positive bacteria and yeasts The nutrient capacity, colour intensity, sensitivity of detection and specificity of colouration, ensure optimum differentiation of mixed cultures. chromID Salmonella enables the detection of serotype Typhi, Paratyphi and most Salmonella Lactose +
S. Enteritidis, P. vulgaris
chromID Salmonella
Ref. Ref.
Selenite F broth
Ref.
Rappaport broth
Ref.
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chromID S. aureus
TM
chromID S. aureus
Incubation: 24 hours S. aureus ATCC 25923
chromID S. aureus
Ref.
Slidex MRSA
Ref.
chromID Strepto B
TM
chromID Strepto B
Incubation: 24 hours S. agalactiae NCTC 8190
chromID Strepto B
Ref. 43 461: kit of 20 plates
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M E D I A F O R S I M U LTA N E O U S I S O L AT I O N A N D I M M E D I AT E I D E N T I F I C AT I O N
chromID O157:H7
TM
chromID O157:H7, an innovative chromogenic medium enabling easy differentiation between the O157:H7 serotype and other microorganisms: identification of E. coli O157:H7 after only 24 hrs of incubation at 37C characteristic green to blue-green colour of E. coli O157:H7 colonies more fertile than SMAC medium, and equally selective inhibition of Gram-positive bacteria and yeasts increase in selectivity with respect to Gram-negative bacteria by adding Cefixime Tellurite, for highly contaminated samples.
chromID O157:H7
Ref.
42 606 6 x 4 ml vials
chromID Vibrio
Incubation: 24 hours V. cholera
chromID Vibrio*
TM
Presumptive identification of V. cholerae is based on the blue-green colouration of beta Galactosidase-producing colonies. Differentiation of V. parahaemolyticus: Pink colonies based on arabinose assimilation.
chromID Vibrio
Ref.
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Fecal culture naturally contaminated by V. cholerae. Images from Dr Scheftel, Institut de Bactriologie, Strasbourg, France.
* Consult your local bioMrieux representative for more product information and availability.
chromID P. aeruginosa*
TM
chromID P. aeruginosa
Incubation: 24 hours P. aeruginosa ATCC 27853
chromID P. aeruginosa
Ref.
* Consult your local bioMrieux representative for more product information and availability.
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* Multidrug-Resistant Organisms
2CHROMOGENIC
M E D I A F O R T H E S C R E E N I N G O F M U LT I D R U G R E S I S TA N T O R G A N I S M S
chromID MRSA
Incubation: 24 hours S. aureus ATCC 43300
in 18-24 hours*
chromID MRSA is dedicated to the surveillance culture or screening of MRSA to identify hospitalized patients requiring isolation. The screening of MRSA carriers with surveillance cultures is a key step in the fight against nosocomial infections. chromID MRSA has been developed to answer this major health concern. Identification of MRSA strains is based on the spontaneous green colouration of -glucosidase-producing colonies (patent pending) and the presence of an antibiotic, cefoxitin. Greater Rapidity: Extremely easy-to-read identification of MRSA = Green colonies after 18-24 hour incubation. Greater Reliability: Excellent performance for the screening of MRSA in terms of nutrient capacity, detection sensitivity and colouration specificity. The presence of cefoxitin enables the detection of MRSA strains including hetero-resistant strains. The selective mixture inhibits most bacteria not belonging to the genus Staphylococcus, as well as yeasts.
Stronger green coloration of colonies when plates read upside down through the plate bottom
MRSA from a nasal swab specimen. Image provided by Sullivan & Nicolaides Pathology, Brisbane, Australia
chromID MRSA
Ref. Ref.
Enrichment broth
Heart-Brain broth Ref. 42 081 kit of 20 tubes Todd-Hewitt broth + antibiotics Ref. 42 116 kit of 20 tubes
Agglutination Tests
Slidex Staph Plus Ref. 73 115 kit of 50 tests Ref. 73 116 kit of 250 tests Slidex MRSA Detection Ref. 73 117 kit of 50 tests
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M E D I A F O R T H E S C R E E N I N G O F M U LT I D R U G R E S I S TA N T O R G A N I S M S
chromID ESBL
TM
Enterobacteria (ESBL)
Incubation: 24 hours E. coli CIP 103982
chromID ESBL
Ref.
Whereas identification to the species or group level is direct, ESBL production must be confirmed by one or more additional tests.
chromID VRE
Incubation: 48 hours E. faecium CCUG 36804, E. faecalis ATCC 51299
chromID VRE
TM
2 for the screening of E. faecium and E. faecalis showing acquired Vancomycin resistance (VRE)
chromID VRE (patents pending) contains two chromogenic substrates (-Glucosidase & -Galactosidase) and Vancomycin (8mg/l) which enable: Direct identification of E. faecium and E. faecalis after 24 hour incubation. Specific and selective isolation & detection of acquired Vancomycin-Resistant enterococci. Characteristic colouration of colonies with: Bluish-green colour = E. faecalis - Violet colour = E. faecium The selective mixture inhibits: - enterococci with natural resistance (ie. E. casseliflavus and E. gallinarum) - most Gram-negative and Gram-positive bacteria as well as yeasts.
chromID VRE
Ref.
E. faecium VRE feces specimen collections
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Heart-Brain broth
Ref.
Vancomycin Bio-DiscsTM
Ref. 54 912 kit of 4 boxes of 50 x 6mm impregnated discs with Vancomycin 30g concentration
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2 B LO O D
Blood agar
Blood agar are isolation media specially designed to facilitate the growth of fastidious microorganisms, Gram-positive bacteria and all the species encountered in clinical specimens.
It contains a peptone mixture particularly adapted to the culture of fastidious microorganisms (streptococci, Listeria...). The presence of blood enables hemolysis determination, which is a basic criterion for the orientation of bacterial identification. Example of characteristic hemolyses: Streptococcus pneumoniae: a-hemolysis, with a greenish colouration around the colony. Streptococcus pyogenes: -hemolysis, with a clear zone around or under the colony. Blood agar can be used to subculture bacterial strains in order to obtain pure cultures.
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2 B LO O D
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TM
Chocolate agar + PolyViteX is an isolation medium particularly recommended for the growth of fastidious strains belonging to the genera Neisseria, Haemophilus, and Streptococcus pneumoniae encountered in clinical specimens. This medium is composed of a nutrient base enriched with factors X (hemin) and V (NAD) provided by the hemoglobin and PolyViteX. This medium can be used to subculture bacterial strains in order to obtain pure cultures.
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2 B LO O D
Storage: shelf-life includes 4 week storage at 15-25C. Chocolate agar + PolyViteX VCAT3
Ref.
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2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Prevention of Perinatal Group B Streptococcal disease 2 GRANADA media for the screening of S. agalactiae
The original principle - Granada formulae are selective and differential media that enable the detection and identification of GBS in clinical specimens. There are 100% specific for GBS. They use Granadaene to differentiate GBS from other pathogenic bacteria. The methotrexate, included in the formulae, increases pigment production. Dr Manuel de la Rosa, creator of the Granada media.
Granada agar
Incubation: 24 hours S. agalactiae ATCC 12386
Granada agar
2 Selective agar for the screening and direct identification of S. agalactiae
Granada agar
Ref.
Incubation: 24 hours Streptococcus B (red to orange characteristic colonies) & Enterococcus faecalis
PSIpettes
Ref. 70
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M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Dermatophytes agar*
Ref.
Incubation: 72 hours A. niger ATCC 16404
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* Consult your local bioMrieux representative for further product information and availability.
Campylosel agar
2 Selective isolation of Campylobacter
Campylosel agar is a selective medium for the isolation of intestinal Campylobacter (C. jejuni and C. coli principally) from stools. The presence of sheep blood favours the growth of the target species. The nutrient capacity is enhanced by the use of reducing agents. Good selectivity is ensured by the antibiotic and antifungal agents present in the medium which inhibit the majority of bacterial and fungal contaminants. Characteristic Campylobacter colonies are small and greyish, and sometimes spread along the inoculation streaks.
Campylosel agar
Incubation: 48 hours Campylo jejuni ATCC 33291
Campylosel agar
Ref.
A7 Mycoplasma agar
2 Selective culture and enumeration of urogenital mycoplasma
A7 Mycoplasma agar is a selective medium for the culture of mycoplasmas, Ureaplasma spp. and Mycoplasma hominis from urogenital specimens. The Ureaplasma urealyticum species has been separated into two new species called Ureaplasma parvum and Ureaplasma urealyticum. For the use of this medium, they should both be considered as Ureaplasma spp. This medium combines a rich nutritive base containing peptones, horse serum and growth factors favouring the growth of mycoplasmas (cysteine, PoliViteXTM, arginine, urea). The appearance of the mycoplasma colonies is as follows: Ureaplasma spp. Sea urchin colonies: round colonies surrounded by a brownish-black precipitate (due to oxidation of manganese sulfate). Mycoplasma hominis Fried egg colonies: round colonies with a protruding center surrounded by a lighter halo. An antibiotic mixture inhibits the growth of most Gram-positive and Gram-negative bacteria contained in the specimen.
A7 Mycoplasma agar
Mycoplasma hominis
A7 Mycoplasma agar
Ref.
Urea-Arginine LYO 2
Ref.
Ureaplasma urealyticum
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2SPECIFIC
M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
SMAC CT agar
Incubation: 24 hours E. coli O157:H7 ATCC 43894
SMAC CT agar
2 Selective isolation of Escherichia coli O157:H7
The SMAC CT agar (Mac Conkey with Sorbitol) enables the detection and differentiation of enterohemorrhagic Escherichia coli of serotype O157:H7, responsible for gastro-intestinal infections or diseases. According to epidemiological data, these types of pathology may also be induced by the O157:H- serotype (non-motile bacteria unlike the O157:H7 serotype). The presence of sorbitol and tellurite enables the differentiation of E. coli O157:H7 which is characterized by colourless colonies with a brown centre. E. coli 0157:H7 strains might also generate colourless colonies with a brown centre. The other E. coli fermenting sorbitol give pink to red colonies. The selectivity for Gram-positive bacteria and certain Enterobacteriaceae is ensured by cefixime, bile salts, crystal violet and tellurite.
SMAC agar
Ref.
BCSA agar
Incubation: 48 hours B. cepacia ATCC 25416
BCSA agar
2 Selective isolation of Burkholderia cepacia
BCSA agar (Burkholderia cepacia Selective Agar) is a medium recommended for the selective isolation and detection of the species Burkholderia cepacia complexe from clinical specimens of mainly respiratory origin (sputum and bronchoalveolar washing) in patients suffering from cystic fibrosis. The presence of a peptone base and sugars enable optimum growth of the microorganisms. Crystal violet and the antibiotics present in the medium inhibit most microbial species (inhibition of Pseudomonas species). A high volume of agar per plate enables good performance for culture at 72 hours.
BCSA agar
Ref.
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Cetrimide agar
2 Selective isolation of Pseudomonas aeruginosa
Cetrimide agar is a selective isolation medium for the detection of Pseudomonas aeruginosa from specimens of various origin. Cetrimide agar stimulates the production of pyocyanin and the fluorescence of P. aeruginosa. Its formula is an optimization of Kings A medium: the addition of a quaternary ammonium inhibits most other microorganisms. Characteristic colonies present a spontaneous pale green pigmentation and green fluorescence under ultraviolet light.
P. aeruginosa ATCC 10145, UV light
Cetrimide agar
Incubation: 24 hours P. aeruginosa ATCC 10145
Cetrimide agar
Ref.
D-Coccosel agar
2 Selective isolation of enterococci and group D streptococci
D-Coccosel agar (Bile Esculin Agar) is used for the selective isolation and differentiation of enterococci and group D streptococci from polymicrobial specimens. The characteristic colonies of enterococci and group D streptococci are colourless or grey and surrounded by a black halo (hydrolysis of esculin). The selectivity of the medium for Gram-negative bacteria is provided by sodium azide. Bile inhibits certain Gram-positive bacteria other than enterococci.
D-Coccosel agar
Incubation: 24 hours E. faecalis ATCC 29212
D-Coccosel agar
Ref.
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M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Legionella GVPC agar has been specially developed for the isolation from respiratory specimens of most Legionella species, notably those responsible for infections: Legionella pneumophila, which is the species most frequently involved (Pontiac fever) but also L. bozemanii, L. longbeachae, L. dumoffii, L. jordanis, L. gormanii, L. anisa and L. micdadei. This selective medium also enables the enumeration of Legionella in water according to standards T90-431 and ISO 11731. The activated charcoal absorbs any toxic substances in the yeast extract and stimulates the growth of Legionella. The ACES buffer stabilizes the pH of the medium at 6.9, which is the optimal pH for growth. The characteristic colonies of Legionella are grey-blue in colour but which can become off-white with age. They have a regular pinkish edge and, when observed under a binocular microscope, have a ground glass appearance. GVPC medium provides excellent inhibition of growth of Gram-positive bacteria, most Gram-negative bacteria, yeasts and moulds, through an optimized combination of three antibiotics.
Gardnerella agar
Incubation: 24 hours G. vaginalis ATCC 14018
Gardnerella agar
2 Selective isolation of Gardnerella vaginalis
Gardnerella agar is a selective isolation medium for the detection of Gardnerella vaginalis in genital specimens. Gardnerella vaginalis, alone or associated with certain anaerobic bacteria (Mobiluncus, Bacteroides and Prevotella), is responsible for various urogenital infections. The presence of human blood favours the growth of the species being tested for and produces -hemolysis around the colonies. -hemolysis obtained solely on human blood agar is highly indicative of G. vaginalis. The antibiotics included in the medium inhibit most Gram-negative contaminants and yeasts.
Gardnerella agar
Ref. Ref.
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M E D I A : S E L E C T I V E I S O L AT I O N F O R S P E C I F I C O R G A N I S M S
Pylori agar
2 Selective isolation of Helicobacter pylori
H. pylori ATCC 43504 (x 2.5)
Pylori agar is a selective isolation medium for the detection of Helicobacter pylori in gastric biopsies. Helicobacter pylori is a cause of gastritis and is associated with the development of gastric and duodenal ulcers. Helicobacter pylori is a microaerophilic bacterium whose viability is reduced on contact with air. These characteristics should be taken into account for transport and culture. The presence of horse plasma and PolyViteX enhances the growth of the target species. The antibiotics present in the medium inhibit the majority of bacterial contaminants.
Pylori agar
Ref.
Portagerm pylori
Ref.
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BCP agar
2 Isolation of common microorganisms
YBCP agar (Lactose agar with Bromcresol purple) is an isolation and differentiation medium for the development of all organisms commonly found in specimens of various origin. It also differentiates lactose-fermenting organisms from non-fermenting organisms. Lactose (+) organisms produce yellow colonies by acidification of the medium. Non-fermenting organisms produce blue or colourless colonies.
BCP agar
Incubation: 24 hours E. coli ATCC 25922 (yellow colonies) & P. vulgaris ATCC 6380 (colourless colonies)
BCP agar
Ref.
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2SIMPLE
M E D I A : I S O L AT I O N O F O R G A N I S M S
MacConkey agar
Ref. Ref.
Drigalski agar
Incubation: 24 hours E. coli ATCC 25922 (yellow colonies) & Salmonella typhimurium ATCC 14028 (blue colonies)
Drigalski agar
2 Selective isolation of Enterobacteriaceae and other Gram-negative bacteria
Drigalski agar is a selective isolation and differentiation medium used to identify Enterobacteriaceae and other Gram-negative bacteria in feces, urine or other biological specimens. Microorganisms that ferment lactose form yellow or yellowish-green colonies; the others produce blue, green, or bluish-green colonies. The presence of sodium deoxycholate and crystal violet inhibits the growth of Gram-positive.
Drigalski agar
Ref.
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SS agar
2 Selective isolation of Salmonella and Shigella
SS agar is a selective isolation and differentiation medium recommended for the detection of Salmonella and Shigella species. The medium is inoculated from stool specimens, suspension of stools or an enrichment broth. The medium detects lactose-fermenting and thiosulfate-reducing (production of H2S) colonies. Lactose-fermenting microorganisms produce pink colonies, the others give colourless colonies. H2S-producing microorganisms produce colonies with a black centre. Colonies of Salmonella are colourless to pale yellow, with or without a black centre. Colonies of Shigella are colourless to pale pink or orange-coloured without a black centre. The inhibition of Gram-positive micro-organisms is obtained by a mixture of bile salts and stains.
SS agar
Incubation: 24 hours Salmonella typhimurium ATCC 14028 (colonies with black centre) & S. flexneri ATCC 12022 (pale pink colonies)
SS agar
Ref. Ref.
Rappaport broth
Ref.
Selenite F broth
Ref.
Hektoen agar
2 Selective isolation of Salmonella and Shigella
Hektoen agar is a selective isolation and differentiation medium recommended for the detection of Salmonella and Shigella species. The medium is inoculated from stool specimens, suspension of stools or an enrichment broth. Microorganisms that ferment one of the three sugars in the medium form yellow or yellowish-pink colonies; the others produce green to bluish-green colonies. H2S-producing microorganisms form colonies with a black centre. Colonies of Salmonella are green to bluish-green, with or without a black centre. Colonies of Shigella are green to bluish-green without a black centre. Inhibition of Gram-positive microorganisms is obtained by a mixture of bile salts and stains.
Hektoen agar
Incubation: 24 hours Salmonella typhimurium ATCC 14028
Hektoen agar
Ref. Ref.
Rappaport broth
Ref.
Selenite F broth
Ref.
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M E D I A : I S O L AT I O N O F O R G A N I S M S
CLED agar
2 Isolation of urinary tract micro-organisms
CLED agar (Cystine Lactose Electrolyte Deficient) is especially useful for the isolation of urinary tract micro-organisms. It also enables the differentiation of lactose-fermenting and non lactose-fermenting bacteria (bromothymol blue indicator). Lactose (+) bacteria produce pale yellow to yellow colonies by acidification of the medium. Non-lactose fermenting bacteria produce green, blue or colourless colonies. The composition of the medium (weak electrolyte content) prevents the swarming of Proteus. The new CLED formula provides real advantages in terms of larger colony size, improvement of Lactose character reading, and better colony differentiation.
CLED agar
Ref. Ref.
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* Consult your local bioMrieux representative for further product information and availability.
2MEDIA
Antibiotics Ampicillin 10 g Cephalothin 30 g Gentamicin 10 g Nitrofurantoin 300 g Trimethoprim / sulfamethoxazole 1.25 / 23.75 g
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2MEDIA
F O R E N V I R O N M E N TA L C O N T R O L S : S U R FA C E S , A I R , W AT E R
surface
Count-Tact range
The reference in environmental microorganism detection and/or enumeration in hospitals
Count-Tact plates are 55mm in diameter and have a grid scored on the base. The convex agar meniscus enables direct application to the test surface, such as walls, floors, utensils, or employees, for hygiene controls. Count-Tact plates are recommended to check critical control points in protected areas when establishing or revising microbiological surface monitoring techniques and programs in hospitals. Count-Tact plates also can be used to control air, notably using an air sampler.
Count-Tact applicator
Count-Tact agar .......................................................................... Ref. 43 501 kit of 20 plates Count-Tact GTS agar without neutralizers ...................................... Ref. 43 582 kit of 20 plates Count-Tact Sabouraud Dextrose Chloramphenicol agar .................. Ref. 43 580 kit of 20 plates For use in clean areas triple-wrapped irradiated media 3P range: Storage at 2-25C Count-Tact 3P Irradiated agar ...................................................... Ref. 43 691 kit of 20 plates ................................................................................................ Ref. 43 699 kit of 100 plates Count-Tact Irradiated agar .......................................................... Ref. 43 491 kit of 20 plates ................................................................................................ Ref. 43 492 kit of 100 plates Count-Tact Irradiated Sabouraud Dextrose Chloramphenicol agar .... Ref. 43 581 kit of 20 plates For use in isolators resistant to disinfection by disinfectant agents (Peroxyd or PerAcetic Acid) Irradiated ISOLATOR Count - Tact 3P agar .................................... Ref. 43 597 kit of 20 plates Equipment (for plates 55mm) The Count-Tact Applicator standardizes surface collections in terms of time and pressure Count-Tact Applicator .................................................................. Ref. 96 300 The Bi-Box is a sterile box for collecting, transporting and incubating Bi-BoxTM .......................................................... Ref. 96 301 10 sterile cases for Count-Tact plates air IDEAL is a aerobiocollector which guarantees effective measurement of aerobiocontamination air IDEAL 3PTM .......................................................................... Ref. 96 303
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For use in clean areas triple-wrapped irradiated media 3P range: Storage at 2-25C
Trypcase Soy 3P Irradiated agar.................................................... Ref. 43 711 kit of 20 plates ................................................................................................ Ref. 43 169 kit of 100 plates Trypcase Soy Irradiated agar ........................................................ Ref. 43 131 kit of 20 plates ................................................................................................ Ref. 43 556 kit of 100 plates ...................................................................................... Ref. 43 557 kit of 20 plates (140mm) Trypcase Soy Irradiated agar with neutralizers .............................. Ref. 43 562 kit of 20 plates Sabouraud Dextrose Irradiated agar ............................................ Ref. 43 554 kit of 20 plates Sabouraud Dextrose Chloramphenicol Irradiated agar .................. Ref. 43 595 kit of 20 plates Equipment (for plates 90mm) The Bi-Box is a sterile box for collecting, transoporting and incubating Bi-BoxTM 90 ................................................................................ Ref. 96 301 10 sterile cases air IDEAL is a aerobiocollector which guarantees effective measurement of aerobiocontamination air IDEAL 3PTM .......................................................................... Ref. 96 302
water
Legionella
Culture media and an identification test in compliance with the ISO 11731, NF T 90-431 and NF T 90-461 standards.
For detection and numeration GVPC agar * .............................................................................. Ref. 43 031 kit of 20 plates ................................................................................................ Ref. 43 032 kit of 100 plates For confirmation of the Legionella genus BCYE with L-cysteine .................................................................. Ref. 43 013 kit of 10 plates BCYE without L-cysteine .............................................................. Ref. 43 023 kit of 10 plates For identification of L.pneumophila et L.anisa SLIDEX Legionella kit ** ............................................................ Ref. 73 120 kit of 50 tests Others Enumeration of total viable aerobic microorganisms R2A agar.................................................................................... Ref. 43 551 kit of 20 plates
* Consult your local bioMrieux representative for further product information and availability. ** Not CE marked
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43 G GGG G
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A True Breakthrough
PREVI Isola is simply streaks ahead in automated specimen inoculation. It revolutionizes front-end media processing tasks by: Maximizing bacteria colony isolation Standardizing plate inoculation & results Streamlining your lab workflow
chromIDTM Candida
Automated inoculation with mixed culture: C. albicans, C. tropicalis, C. krusei
chromIDTM CPS
Automated inoculation E. coli ATCC 25922
Streaking Ahead
An original, revolutionary applicator using high precision technology standardizes and maximizes colony inoculation: Maximum agar surface inoculated Optimum, pressure-controlled contact with the agar while inoculating Standard quantity of inoculum used every time This simple device is at the heart of PREVI Isola. It will change the way you think about inoculation and laboratory workflow.
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Certain categories have been grouped together, particularly as far as specimen collections are concerned. For example, the "genital protocol" is for both men and women. The analysis procedure proposed should therefore be adapted to the specimen to be studied as not all the microorganisms indicated will necessarily be tested for. Teaming our simple and practical protocols with our extensive range of quality media provides you with an optimised workflow solution.
2 microorganisms tested 2 the examination performed 2 epidemiological data 2 cost / workflow ratio
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G 46
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Color Gram 2
2 U R I N E P ROTOCOL
Slide/coverslide
Gr.B Streptococcus
II
III
CLED
Lwenstein Jensen
Coletsos
IV
BCP
OR
EMB
OR Drigalski
Mac Conkey
Columbia
GG
G G
G 48
G
Color Gram 2
2 F EC AL P ROTOCOL
SPECIFIC ISOLATION Campylobacter E. coli E. coli O157H7 Clostridium difficile Vibrio Rotavirus Adenovirus
Shigella
Yersinia
I
Hektoen chromID Salmonella Hektoen Yersinia CIN Campylosel BCP SMAC CT C. difficile chromIDTM Vibrio
C T
VIKIATM Rota-Adeno
II
III
Mac Conkey
hCG 10
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G
G
G
Color Gram 2
2 G E N I TA L P R O T O C O L
PortagermTM
Giemsa Slide/coverslide
Streptococcus / Staphylococcus
Columbia CNA chromIDTM S.aureus
Gardnerella
Enterobacteriaceae Non-Enterobacteriaceae
Mac Conkey
Yeasts
chromID Candida
Mycoplasma
Chlamydiae
Gardnerella
Mycoplasma IST 2
II
Chocolate PolyVitex
Columbia CNA
Columbia or TSA
BCP
III
Gonoline Duo 2
Listeria
Columbia CNA
Schaedler
II
Chocolate PolyVitex
Columbia or TSA
Granada
Columbia or TSA
GG
G G
G 50
G
2 S U P P U R AT I O N P R O T O C O L
Color Gram 2
Schaedler
chromIDTM S. aureus
Columbia CNA
Mac Conkey
Schaedler Vit. K3
Chocolate PolyVitexTM
chromID Candida
Columbia CNA
Columbia Sheep
II
OR Columbia CNA
III
Columbia CNA
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G
G
G
2 T H R O AT P R O T O C O L
Color Gram 2
Columbia CNA
chromIDTM S. aureus
Chocolate PolyVitexTM
chromID Candida
Columbia CNA
Schaedler
Haemophilus Chocolate 2
II
TSA
TSA
III
Loeffler
GG
G G
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G
Color Gram 2
2 S P UTU M P ROTOCOL
Staphylococcus
Enterobacteriaceae Non-Enterobacteriaceae
Mac Conkey
Haemophilus
Yeasts
Mycobacteria
chromIDTM S.aureus
Haemophilus Chocolate 2
chromIDTM Candida
II
Lwenstein Jensen
Coletsos
Neisseria Branhamella
Legionella
Burkholderia cepacia
P. aeruginosa
RSV
I
Schaedler Sab Genta Chloram 2 Chocolate PolyVitex GVPC BCSA chromID P. aeruginosa RSV Direct IF OR QuickVue RSV Test
PortagermTM
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G
G
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2 B L O O D C U LT U R E P R O T O C O L
Color Gram 2
SYSTEMATIC ISOLATION Enterobacteriaceae NonEnterobacteriaceae Streptococcus / Enterococcus Staphylococcus Corynebacterium / Listeria Campylobacter Neisseria / Haemophilus Anaerobes
Slidex Pneumo-Kit
Columbia or TSA
Chocolate PolyVitexTM
Schaedler
Brucella
III
TSA
III
GG
G G
G 54
G
Color Gram 2
Listeria
Staphylococcus
Mycobacteria
Ziehl Kinyoun-Gabett Slidex Meningite Kit 5 Slidex Meningite Strepto B Slidex Meningite MnB / E. coli K1
India Ink
Chocolate PolyVitexTM
Columbia or TSA
Mac Conkey
Lwenstein Jensen
Coletsos
chromIDTM Candida
II
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Notes
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04-09 / 002GB99095B / This document is not legally binding. bioMrieux reserves the right to modify specifications without notice / BIOMERIEUX, the blue logo, API, air Ideal 3P, Bi-Box, BioBall, Bio-discs, chromID, Count-Tact, CPS, Hemoline, PolyVitex, Portagerm, PREVI, Slidex, Uriline, Vikia are used, pending and/or registered trademarks belonging to bioMrieux S.A. or one of its subsidiaries / QuickVue is a trademark belonging to Quidel Corporation / ESwab is a trademark belonging to Copan / Any other name or trademark is the property of its respective owner / bioMrieux SA RCS Lyon 673 620 399 / Photos: bioMrieux, N. Bouchut. Printed in France / THERA Conseil / RCS Lyon B 398 160 242.
bioMrieux S.A. 69280 Marcy lEtoile France Tel. : 33 (0)4 78 87 20 00 Fax : 33 (0)4 78 87 20 90
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