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PAPER B 40

INSTRUCTIONS: 1. All question Carry 1 Mark Each 3. Overwriting is not allowed Choose the correct alternate: -

COMPETITION LEVEL
BIOTECHNOLOGY
2.1/4 marks will be deducted for wrong answer

M.M

1.

Autonomously replicating chromosomal DNA in called a) vector c) Endonulclease b) cloning d) Plasmid

circular

extra

c) gel electro phorosin d) None of the these 10. DNA fragments are separated by forcing them to move towards the anode because DNA fragment contain. a) Pozitine charge c) Neufral charge a) Agar Agar c) Lithiose b) Negative charge d) 1 & 2 b) Methy d) Agarose.

2. The linking of antibiotic Resistance gene with the plasmid vector become possible with the enzyme a) DNA ligase c) Endonuclease b) Rmase d) Lipase

11. Most Commonly used matrix for gel electrophoresis

3. The ability to multiply copies of antibiotic resistance gene in E Coli was called a) Recombined c) Gene framfer a) E CORI c) Bam I b) Cloning d) Gene plasmid b) Hind III d) Hend II

12. The separated DNA fragment can be visualized only after staining the DNA with a compound know as. a) Ethidium bromide c) Sodium 13. Elution is. a) Extraction of DNA from gel piece b) Extraction of DNA from plasmid c) Insertion of DNA in to gel piece d) Insertion of DNA in to plasmid 14. Which among the following are good selectable marker. a) Ampicillin c) Tetracycline b) both a and b d) All of the above b) Bromedium d) Chromadim

4. The first restriction endonuclease in

5. Restriction enzymes lake EcoRI, Naming these enzymes is the first letter of the name comes from a) Genus c) family b) Species d) class

6. It endonucleases cut specific position of DNA what exonucleases do a) cut RNA piece b) Joint DNA piece c) Remove nucleotide from the end of DNA d) Add nucleotide from the end of DNA. 7. The same word in read in both dissection is called. a) Polindrome DNA c) Cyclic DNA 8. Palindrome DNA is a) 5CATACG3, 3GATGC5 b) 5GAATTC5, 3CTTAAG5 c) AATTCCGG , TTAAGGCC d) GAATTC , CTTAAG 9. DNA fragments can be separated by a technique known as a) microphorosin b) clearnes
3 3 5 5 3 5 5 5

15. Cells are bombarded with high velocity micro particle of gold or tungsten coated with DNA in a method known as a) Biolistics c) Both a & b b) Gene gun d) DNA gun

b) Plasmid d) All of the above

16. DNA is a lydrophilic molecule it con not pass through cell membrane to take up the plasmid in to bacteria bacterial cell must be treating with a) univalent Cation b) Divalent cation c) Tetravalent cation d) Quadri cation 17. PCR stand for polymerase chain reaction involve a) Devaluation c) Degradation b) Annealing d) None of these

18. Recognition site of EcoR I is


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BRC INSTITUTE OF COMPETITIONS S.C.O 55-56, Sector 20-C, CHANDIGARH PH : 0172-2775243, 2774390

a) 3 GAATTC 5 c) 3 GTTAAC 5

b) 5 GAATTC 3 d) 53 GTTAAC 3

29. Gene gun method is used for : a) monocot c) both a & b 30. BAC stands for: a) bacterial artificial chromosome b) bacterial acidic culture c) bacterial alkaline composition d) all of these 31. Isolation of DNA from cell can be done by using a) ligase c) cellulose b) lysozyme d) pectinase b) dicot d) gymnosperm

19. Which of the following is the product of traditional biotechnology a) Idli c) Jalebi 20. In EcoRI , co denotes: a) genus c) strain b) species d) order of discovery b) Dosa d) all of these

21. Which of the following is reporter enzyme: a) DNA polymerase b)alkaline phosphatase c) Leuciferase d) all of the above 22. Which of the pair belongs to ampicillin resistance gene a) Pvu I and Pst I c) BamH I and SalI b) Pvu I and BamH I d) Pvu II and Pvu I

32. Amplification of gene of interest can be done by: a) DNA finger printing b) PCR c) YAC a) DNA templates c) enzyme a) bacteria c) virus a) 10C c) 80C a) 1 c) 3 d) yeast b) Primer d) all of these b) fungus d) protozoa b) 20C d) 72 b) 2 d) 4 33. What are the requirements of PCR:

23. Which of the following vector is used for DNA sequencing and site directed mutagenesis: a) BAC c) Bacteriophage a) Name of scientist b) YAC d) M13 b) Name of school

34. Taq polymerase enzymes is obtained from

24. In PBR 322 B & R stands for: c) Name of university d) Name of city 25. Which of the following vector is used for gene transfer in eukaryotic genome: a) Plasmid c) BAC b) cosmid d) YAC

35. What is the optimum temperature in PCR

36. How many steps are involved in completion of PCR

26. Which of the bacteria is widely used in gene transfer in plants: a) diplococcous c) agrobacterium 27. A shuttle vector has a) one site each for replication and recognition b) two site each for replication and recognition c) one site each for replication and selectable marker d) two site each for replication and selectable marker 28. Competent host can be formed by : a) sodium c) calcium b) potassium d) chlorine b) spirillum d) none of these

37. Which of the following is not the application of PCR a) DNA finger printing b) Prenatal diagnosis c) Diagnosis of specific mutation d) sed development 38. Commonly used bioreactor used is a) Sparged stirred tank b)simple stirred tank c) compound stirred tank d) complex stirred tank 39. Which is the sweetest protein: a) amylase c) both a & b a) interferon c) hirudin b) brazzein d) none of these b) insulin d) asparagina

40. Which of the following is an anticoagulant:

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BRC INSTITUTE OF COMPETITIONS S.C.O 55-56, Sector 20-C, CHANDIGARH PH : 0172-2775243, 2774390

ANSWERS
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. D A B C A C A B C B 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. D A A B C B B A D B 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. C A D A D C D C A A 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. B B D A D C D B B C

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BRC INSTITUTE OF COMPETITIONS S.C.O 55-56, Sector 20-C, CHANDIGARH PH : 0172-2775243, 2774390

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