M.Sc.

BI 1 - Cell Biology & Genetics

Date: 7-Jan-10 Full Marks: 60

Part A
1. A. B. C. D. Ans: B 2. A. B. C. D. Ans: D 3. A. B. C. D. Ans: C. 4. A. B. C. D. Ans: D The thickness of the double membrane sheath of the nuclear membrane is ____. 8.5 nm 7.5 nm 9.3 nm 2.5 nm

The bacterial cell wall is made up of _________. cellulose glycogen glucoronic acid peptidoglycan

The outer membrane is often studded with ______. R.E.R S.E.R Ribosomes All of them.

Peptidoglycans in the bacterial cell wall is seen as a complex network formed by ______ N-acetyl glucosamine Glucosamine sulfate N-acetyl muramic acid Both A and C.

5. In Drosophila, the pattern is established: A. During production of the oocyte. B. As a result of environmental interactions with the oocyte. C. During gastrulation. D. After embryogenesis has completed. Ans: A. 6. Microtubules isolated from cells, particularly brain tissue, contain additional proteins called _______. A. -tubulin. B. -tubulin C. Both A and B. D. Microtubule-associated proteins. Ans: D.

7. A. B. C. D. Ans: B. 8. A. B. C. D. Ans: C 9. A. B. C. D. Ans: B. 10. A. B. C. D. Ans: C.

______ is the energy source for microtubular assembly. ATP GTP NADP None of the above.

Proteins, which have been found to be responsible for the movement of vesicles are ______. Kinesins Dyneins. Both A and B. Tubulins.

Cilium or flagellum emerges from a basal body, which is known as ________. Centriole. Kinetosome. Axonema Dynein.

The direction and the rate of ciliary or flagellar beat is regulated by _______. Calcium ions Cyclic AMP. Both A and B. G protein.

11. _______ is permanently attached to one cytoskeletal element and moves it with respect to a neighboring element. A. Actin. B. Kinetochore. C. Dynein D. Tubulins. Ans: C. 12. There is a definite proportion in the size of the nucleus and the amount of cytoplasm and this proportion is expressed as the ___________. A. cytoplasmic index B. Nucleoplasmic index C. Syncitium. D. None of the above. Ans: B.

13. ER is mainly involved in __________. A. Post-translatory modifications and synthesis of derivatives of several macromolecules B. Intracellular transport and storage of materials. C. Synthesizing lipids, glycogen, cholesterol, glycerides, enzymes and hormones. D. All of the above. Ans: D 14. In which part of cell, anaerobic breakdown of glucose takes place. A. B. C. D. Ans: C. 15. Bacterial DNA is also known as _______ A. Naked DNA B. Nucleoid. C. Circular DNA D. All of the above. Ans: D 16. ______ are principal sites of respiratory enzymes in bacteria and hence in the synthesis of cell wall and also co-ordinate events related to bacterial cell multiplication. A. B. C. D. Mitochondria Mesosomes Ribosomes None of the above. Mitochondria Endoplasmic reticulum Cytoplasm Vesicles

Ans: B 17. Peptidoglycans in the bacterial cell wall is seen as a complex network formed by ________ . A. teichoic acid B. N-acetyl glucosamine. C. N-acetyl muramic acid molecules. D. Both B and C Ans: D

18. Outside the living cell, virus exists as a particle known as _____. A. B. C. D. Ans: C 19. Sturtevant defined one genetic map unit (m.u.) as _________. A. B. C. D. Ans: C 20. Nuclear pore complexes are the sites for exchange of macromolecules between _____ and the _______. A. Nucleus and ER. B. Ribosome and Smooth Endoplasmic Reticulum. C. Cytoplasm and the nucleus. D. Golgi bodies and vesicles. Ans: C 21. A. B. C. D. Ans: D. 22. A. B. C. D. Ans: A. 23. A. B. C. D. The set of homologous chromosomes of an organism is known as ________. Chromatin. Ideogram. Euploidy Karyotype 4 Export and import of proteins and ribo-nucleoprotins are facilitated by ________. Signal sequences of amino acids in the proteins. Signal complex. Signal transduction. Energy transduction. RNAs are exported in the form of ___________. Rev proteins Nuclear export sequence. Specific receptor nucleoporins. Ribonucleoproteins. that distance between genes for which one product of meiosis in 100 is recombinant. Recombinant frequency. Centrimorgan. Morgan. Prion molecule. Bacteriophage Virion molecule. Viroids.

Ans: D. 24. When the change in chromosome number does not involve the entire set of chromosomes, the situation is called ______. A. Polyploidy. B. Aneuploidy. C. Chromonemata. D. None of the above. Ans: B. 25. A. B. C. D. Ans: D 26. A. B. C. D. Ans: B. 27. A. B. C. D. Ans: C 28. A. B. C. D. Ans: B. 29. The second filial generation of Mendels monohybrid cross gives phenotypically _________. A. 1:1:1:1. The ______ is a huge molecular complex having 100-200 polypeptides. Non-protein complex. Nuclear pore complex. Nuclear poration complex. None of the above. DNA replication occurs only in a specific period of interphase, which is called _____ G1 G2 S Interphase. What percentage of DNA and Histones are found in the deoxyribonucleoprotein ? 35% DNA and 65% of histones. 45% DNA and 55% of histones. 25% DNA and 75% of histones. None of the above. Which model holds that the chromosome is a tightly homogenous fibre? Multistrand model. Singlestrand model. Unfolded fibre model. Folded fibre model.

B. 1:2:1. C. 3:1 D. 9:3:3:1 Ans: C 30. A. B. C. D. Ans: C. 31. The two genes (T and t) representing the two alternative (tall and dwarf) of a particular character (namely height) are present on two separate chromosomes, known as _______. A. B. C. D. Ans: B. 32. Genes which express characters in only one sex are called _______. A. B. C. D. Ans: A. Sex limited gene. Sex influenced gene. Diagynic. None of the above. Heterozygous chromosomes. Homozygous chromosomes. Hemizygous chromosomes. None of the above. In regard to development, the term pattern refers to: The proteins produced by an individual cell. The stages of development through which an organism proceeds. The arrangement of the regions of the body of an organism. The process of differentiation of specific cell types.

Part B
33. Which of the followings are associated with scaffold, which are component of Nuclear Pore Complex? i) ii) iii) iv) The cytoplasmic ring. The nucleoplasmic ring. The central ring. The scaffold ring.

A. Only (i), (ii) and (iv) B. Only (ii), (iii) and (iv) C. Only (i), (iii) and (iv)

D. Only (i), (ii) and (iii) Ans: D 34. Which of the following mechanisms repair double strand breaks? (i) Photo reactivation (ii) Homologous recombination (iii) Mismatch repair (iv) SOS A. B. C. D. Ans: C. 35. In the nucleolus, the pre-rRNA or 45S RNA gives ______ i) ii) iii) iv) A. B. C. D. Ans: A 36. What are the different types of coiling seen in Chromosomal threads? i) ii) iii) iv) A. B. C. D. Ans: C 37. Based on the position of centromeres, chromosomes can be classified as _____. Paranemic Plectonemic Tectonemic Orthonemic Only (i), (ii), and (iii). Only (ii), (iii) and (iv) Only (i) and (ii) Only (iii) and (iv) 28S 18S 5.8S 41S Only (i), (ii), and (iii) Only (ii), (iii) and (iv) All of the above. None of the above Only (i) and (ii) Only (ii) and (iii) Only (ii) and (iv) Only (ii), (iii) and (iv)

i) ii) iii) iv) A. B. C. D. Ans: C

Telocentric Acrocentric Submetacentric Metacentric Only (i), (ii), and (iii) Only (ii), (iii) and (iv) All of the above. Only (ii) and (iv)

38. _______ is the radiation source, which induces temperate phage, a non-virulent to become virulent one. i) ii) iii) iv) A. B. C. D. Ans: C. 39. Homologous recombination and site specific recombination requires ______. (i) DNA transposition (ii) Illegitimate recombination. (iii) Rec gene (iv) Homologous base sequences. A. Only (i). B. Only (i) and (ii). C. Only (iii) and (iv). D. Only (ii) and (iii). Ans: C. 40. The excision repair process may begin by recognition of a damaged nucleotide or by recognition of a damaged base. Damaged bases, often a consequence of chemical mutagens, are recognized by a variety of cell enzymes called (i) Glycosylases (ii) photoreactivating enzymes (iii) ligases Gamma radiation. X-ray radiation Ultra violet radiation Atomic radiation. Only (i) Only (i) and (ii) Only (i), (ii) and (iii). Only (i) and (iv).

(iv) peroxidases A. Only (i) B. Both (i) and (iii) C. Only (ii) and (iv) D. Only (ii) and (i) Ans: A.

Part C
41. Match the process in Set I with the function in Set II: Set I: 1. 2. 3. 4. Nuclear envelope. Nucleo-cytoplasmic transport. Signal sequence Nuclear Transporter

Set II: a) It is the central hub of Nuclear Pore Complex, which carries out the outward and inward transport of proteins and RNAs. b) Plays an active role in nucleo-cytoplasmic interaction. c) Act as nuclear localization signal, which enable the proteins to pass through the nuclear pore complexes, by docking with specific nuclear localization signal receptors of the nucleopore complex. d) Involves both import to and export from the nucleus. A. 1-c, 2-a, 3-d, d-b. B. 1-b, 2-d, 3-c, 4-a. C. 1-d, 2-b, 3-a, 4-c D. 1-a, 2-c, 3-b, 4-d Ans: B 42. Match the four components of Nucleolus of Set I with their functions in Set II: Set I: 1. 2. 3. 4. Amorphous matrix Chromatin Granules Fribrillar centres.

Set II: a. Involved in processing and maturation of pre-ribosomal particles. b. Transcriptional site of rRNA genes. c. It is site for distribution of granules and fibrils.

d. Serves as a template for RNA synthesis. A. 1-b, 2-c, 3-d, 4-a. B. 1-d, 2-a, 3-b, 4-c C. 1-c, 2-d, 3-a, 4-b. D. 1-a, 2-b, 4-c, 4-d Ans: C. 43. 5 out of 1000 progenies from ABC/abc X abc mating are double cross over type. Frequency of recombination between A & B is 10%. What is the expected frequency of recombination between B & C if interference is zero?

10