Regulacin y Funcionalidad del gen Rnf19 en Espermatognesis

Rnf19 (XYbp)

Das Postnatales:

10

12

14

15 16 17 18 19 20 22 Ad

4.2 KB 2.8 KB

Actina

Promotor Rnf19

eGFP

43C

3h
EGFP P.C. EGFP

6h
P.C.

C HS 1

HS

HS 2 HS

Protena Rnf19

Rnf19 (840 aa y 90 KDa)

RING-finger Regiones de localizacin nuclear Regiones transmembrana

MQEQEISFIFKYNEGLCMNIDSDSILMSILDMSLHQQMGSDRDLQSSTSSVSLPSVKKAP KQRRISIGSLFRRKKDSKRKSRELNGGVDGIASIESIHSEMCADKNSIFSTNTSSDNGLTSI SKQIGDFIECPLCLLRHSKDRFPDIMTCHHRSCVDCLRQYLRIEISESRVNISCPECTERFN PHDIRLILSDDVLMEKYEEFMLRRWLVADPDCRWCPAPDCGYAVIAFGCASCPKLTCG REGCGTEFCYHCKQIWHPNQTCDAARQERAQSLRLRTIRSSSISYSQESGAAADDIKPCP RCAAYIIKMNDGSCNHMTCAVCGCEFCWLCMKEISDLHYLSPSGCTFWGKKPWSRKK KILWQLGTLVGAPVGIALIAGIAIPAMIIGIPVYVGRKIHNRYEGKDVSKHKRNLAIAGGVT LSVIVSPVVAAVTVGIGVPIMLAYVYGVVPISLCRSGGCGVSAGNGKGVRIEFDDENDI NVGGTNAAIDTTSVAEARHNPSIGEGSVGGLTGSLSASGSHMDRIGTIRDNLSETASTM ALAGASITGSLSGSAMVNCFNRLEVQADVQKERCSLSGESGTVSLGTVSDNASTKAMA GSILNSYIPLDREGNSMEVQVDIESKPFKFRHNSGSSSVDDSGATRGHTGGASSGLPEGK SSATKWSKEATGGKKSKSGKLRKKGNMKINETREDMDAQLLEQQSTNSSEFEAPSLSD SMPSVADSHSSHFSEFSCSDLESMRTSCSHGSSDCHARFTAVNTLPEVENDRLENSPHQ CSSALLSKAASCSDVPQPSHAADEHGTSRSGGKPMVDLCFGDALRETNNNHSHQTADL KVAVQTEI

Funcionalidad de la construccin Red

CMV

DsRed

3UTR Rnf19 2.8 kb