Documentos de Académico
Documentos de Profesional
Documentos de Cultura
List of Courses
SL No. r-
Course 'O~'
I COoli rsc Ti lie
-t-
Remarks
lst Semeste'f (15,t Ve,ar)
I Cd I Biologv
-y
1- -1
,2nd S,emester
7
Il 1I
Ilin-Mnlecu le~,
Mirrnbial PhysiDlom' & .Gt'ndK~ Computer applications &: Biostatistics
Laboratory> 1
Molecular Bin-logy
Macromolecules & Busic Enzymology J:)io.logy ·of the Immune System Biophvsical Chemistry
Elective paper
Laboratory - [I
3rd Semester (2nd Year)
12 13 14 lr:;:,
16
4th Semester
17
A nima I Bi( rtechuol (logy Gene-tic Engineering [Jla1"'1l Hi'ClhtcbnolDgy Student Seminar T'rorcct
Bioproccss Engin.;:otonng & T«>(hnology Cf'_nnrnic~ and Functional Cenomics r-Jt'clin~ Paper
Project
40 Lectures - J Cred i t~
40 I.eO u rlO~ - ,j C red! is
40 Lectures - :1 Credits (Practica) Theory )-2 Cred i ts. This ito nut t'1 structured I..:CiHrs,(::', It will be [J combination of theorv & practicals
5 Credits
40 Lectu res - 3 Credits 411 Lectures - 3 C red j ts 40 Ledurefl- 3 ere 1i ts 40 Lectures - 3 Creditt; 2.Cn.?'d.itb
5~redit::.
40 Lecru 1"(tS - ;) C redi hi ·m J .ed1.uCs - 3 Credits.:to Lectur - 3C;reditb 1 Cr\!d it
6, Credits
40 I ,(>CHU'l.:S - :3 C HJ:d its 2':;' Lectures - 2: Credits 2 Credit
6 Credits
Elective Paper
f:.ach student .. vill "Opt For u comprehensive. jn(er.l.c.tiv~ course with eric of the faculty members. Th.1.:' topic lyf specialixaticn and course contents vim be determined by' trw supervisor
Project
Th pr.pject will he, based upon research and actual bench work. 11 will begin from the 1rd semester L1nd wil.J continue through the fourth one, The project report \·.".iU be submitted at the end of the -lth semester an~-{ evaluated.
Student Semina r
larh student under lh~ supervistun of a faculty member will deliver a (" mprehensivc seminar, which will be evaluated. I he topic normally \>\'ill be from an cmel'gi"ng ·dn~Si of Modern Biolugy" Biome d ical, j] i 01 ec hno logy 0 r i ts <l r p ll ca tj oris.
Independent practicels 1m,,), be held under each course. However. for examination purposes <1. :.:;ingki cn rn prchensive 2.-3 days prart t(.11 be he I (I for eac h sum '~5ler, covering different courses offered during Ulrl t semester, [t is recurn mended tha t practical tral ning be fJlrg.;:lnizc:d as <In exercise rather than (l simple demonstration. The student rn ust actual! y perform theexperi merits.
Asaignrnente
Rer,ular <sslgnments must he give.n to each st udcrit du ring whole sernester j n each course. At:~jgnrn(:'n.t'S should DC relevant tn the course contents. All assignments must be evaluated tIS mterna! ~!!>::}e:-;)o III f' n t.
Invited lectures frnrn eminent researchers, industrialists arid nthers on recent issues related to b i odi vcrsitv. ethics, b lo-sufety. intellectual property righlli, paten ~ issues, and good laboratory and manufactu ring practices wi II be ,t)rg~·m ized.
It is also proposed to in("'hul~~ the followingareas <IS parts of 'student seminar', 'elective papers' and 'af,:::::jgnrnl?nt~' •
1. Pruteorn ics, fu nctional gerH.')m ics zma therapeutic gE'nomics.
"] Topics lHL humar: g'E!ntl'lks and gctH;.·bc drssection of heritable dlseascs-anddetection of associated mutations and diilAnostic~ based on I?CI~.
3. Technologies being adapted for future application like cloning of animals with a c<lpacily to produce hu man macromolecules.
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COURSES
1 st Se m ester
SI. COlilTSC Titfe
1 Cell biology
Theory
1.
Di \·,t'tsity of cell size and shape. Cell thcorv.
Structu re of l'rokaeyotic and eukaryntic eel ls - isclation . and grow th {If cells. f'v1icroscnpi(.' techniques fu study of cells"
Sub-cellular frActiunatwfl and criteria. of functional inte.gruly_
Cellular organcl1es.-p12lsma membrane, cell walt their structural (ngan~7ati0·n. T mnsporl of n utrients, ion - and h}<l( rornolecules ~H::roS5 membranes.
Cellular ~ncrg,:-{ transacttons - role of mitochondria and chloroplast.
CcU cvcle - rnolecu lar events and modcl svsterns.
- - ,
J.
-l.
7.
, ,
9.
1 o.
Cellular responses to environmental signals in plants and animals - mechanisms of signa I transdur ti.on ..
C(~] 1 !Tltoli 1 ily- cilia, flagella of eukaryetcs and pro karyo tel;,:. Bh 11ugy l,f GlT'ICel'.
1 J
12_
1 _i. Metahol ill', path W<l_Ys <U'l:d th.ei r regula hun.
H.. Bio ynthesis of proteins i'li Euk'IIYoUc cell, Co-and post-translaticnal mo Iification. intracellular protein traffic.
15 _ Cel III lar ba sic of d i H'eren t iatio nand develop rnent - rm (0<;1.';, gam e.tngt I1C'S:iS and fcr trlization. Development in drosophila and arabidcpsis: spatial and ternporary r~gu la Uoj1 uf gene expression.
16. l3rid introduction to the .IHe cycle and molecular bic.ilugy of some important pathogens of AiDS, Mal a rbi' I IEp,tti tis, Tuberculosis, Filaria, Ka laza '",
17 [Jl'otei 11 'I oca HZ<1l.ion : sy nth esis of. secretory and membrane pro reins. import into nucleus, mitochondria, c1110 roplas] and peroxisome . receptor mcdiatedendncyto is.
Practicals
1 . Micrcscopy.: bright field. phase eontrast and fluorescence micros .opy.
2:. Mic t\I~Clt~' y.
J'. r nstrumetttal methods f rr cell. biology.
-I:. Sub cellular fractionation and marker enzymes.
t)., H istochemica) techniques,
6. Mitosis and \1eiw:;is_
1...
Molecul<lr Blulogy of Cell, Alberts, H et el. MOJ~CLlIa. (_f'!1 111ologo' Lodish et, al.
Reproduction in Eukaryotic cells, DM Prescott .. I\cadf:mil- F'r€_'i-s D~\,p.l()plm~ntal Biclogy, SF Cilbert, Slnauer Associates Inc
Cell In Devcloprncnt and lnheritanre. EB'"Vibml, Macfvlilan, Nev York. The Coiled Spring .. , Eth<lo Hier, .Cold Spring Harbor l'u.>s _
Ft"ftHi!ati(in, FT LlJng(~"Chapman and F-!~Il.
Molecular Blolo{;';v of Steroid and Nuclear Hot-moue RtY,t:Op. I"t.HS. LP Freedman
".
}_
I.
8-
Hirkhuser.
2 Bio-Moh=,eu les
Theory
1. Chern leal tcundaticns or biology - p H, P K, acids .. bases. buffers, wea k bonds anc u~\":alent bonds.
2. r')rfnciples of thermodynamics ..
3. Classes of rganic corn pounds ;;lTld +unctlonal groups -atomic and molecul,n
dirnenaions, space fillirtgaJ:"ld ball and "tick models, .
• l. Amine acids and peptides > classi fk~hoi1, chemical reactions and physics I properties
5, Sugars - classification and reactions.
h. f Ietcrocyclic compounds and s~cor'1dary metabolites in living svsterns > ni-wl eotid I2S
pigments <lind tsoprenoides,
r, Sl"fYH'Mion techniques lor different biornolecu les_
8, Lipids . classification. structure and functions.
9-. F)roh:;,ins- cl assification and separation, puri fi'c-aUon ;ll1d criteria of homogenei ty, ~.I:1(: gW1JP analvsis, hierarchv in structn re and Ramachandran map.
lLL I'olysaccharides - typc~, structural features, methods for compositional analysis.
II, Analytical techniques in hiochernistry arid biophysics for small m()i.f;l(ulE:;'s ana rnacrornolecu les for quarttisation.
P':ra . ctl C?lts
1, Titration of <Imino adds.
1. 'Colorintetric determination of pK
:0;.. M~ .. del building u Stll£. space, fllUJl,g/baJl and stick models,
i!; .RC:'2J.ction~ of amino acids, sug.J.TS and lipids, including 9!(;I:gn~~j1tk tests,
5, ~P;0bUon! pLll'i ty determination and quanlisaHon of chohilsteroL IJN A and RN A:.
h, Quantisa tion of proteins and sugrir5,
7, Anal ysb .O[ oils-iodine 'number, sa'~)onillc<~t:ivn value and acid number.
8. "U'V; visible, fluorescence and nt spectroscopy and absorption spectra
tj Separation techniques - cenlriiugati(1P1. ChrOj1!1atogr,:!]2Ihy {gel p'E!~'n,ea,Eon, ion ex cha'tige, Tl.C, etc.), and d~dmp_horesb (PAc:;E-SDS A~P AGAROSE),
10 Cleavage of DN A with rcstricticn enzymes and electrophoresis or frsgm.ents.
1 1. La helltr'lf'j o+ DN 1'\,1 EN {-'It. Fc;i<'lsmitl isolatl on,
I, Bio(h.ernical rakulc:itionSt Irwinn H Se:ge], Joh,,.· WHey and Sons lnc
2. .GencH'tl Chemistry, Linus Pauling, W, H. Freeman &. Company.
}, C)~'ga:llk Chemistry, DJ Cram 'and ·GS Ham tl).9n:d~ M~'GrflV'" H [1 L
4. Bi6(hemlstry~ D, Veer 0-nd J, G, Voet, J. Wiley & Sans,
5". Physical Biochsm is try, C). Freifilder, W.H.r.reenIn:n .&;_. ·Corn.p.';l.ny.
fl. IJ:1.bo ra tory Techniques in Bio(h~IJtistry ·and 1vIolecultu Biology, Wm'k 2lndl Work..
7 Understanding Chemistty, C\J.R Rapf U)llVersiti.es Press! Hy{~~[<.abad,]9Q9,.
cL A Uiologist\; GuLde lo I'rtnclplesand Techniques of Practical B-inchembtry, K.Wibon &: K. IT. Gql]ldil'1g, ElBS Edition, 19t'l6,
y - -] :D'O I ~ of Bl(lche mist ry b y T~. C ooper,
3 M~crobia I Phys iology and Genetics
Thea.x)'
1- The l3eginning ef .\!1ic:robiol.ogy . The discovery of lhf' microbial world hy AI1~ony V!lE1.
Leeuwenhoek: Th-e 1CQPtroY'Nsy o¥:ir ~rDJ1.t~'{lW\.lU~ geT''leTo.:b.nn, role uf micrnorgartisms in transformabon of m-ga.rri[ matter and in. [he causation of diseases: development of pure culture rn~th.l)~:ls; the enrichment culture methods and dcvclupment of microbiology in the twentieth century,
2 J\f~ ~h ods in ;\lfjCI'citiJology ~ pu re cu It ~HE> techni queSt the th~'br.'ji and practice uf ste rll j aauon: pr inci ples of microbi a] n utr i tion: construct! on of C1J lture med i <I; enrichment culture techniques for isolation 0f chernoa urot-rophs, chemoheterotrophs '<:1 ned F~hotO'~Yl~tlwtiC" microorganisms.
-
GENERAE
3, !'v1krubiJ] Evolution, Sy~tematjc~ <.md Taxonomy : The evolution. of earth (md the earliest 19 fc forms. primitive org?l nlsms and theirmetabolic str<lte-gie" and rnelccula rending: new approaches 1·0 bacterial. taxonomy classification. including ribolvptng; ri bo surnal RNA sequcncmg: chaructcr istics ()I: primary domains; l-\J xoruirnic nomenclature and U·'I.'gen's Manual,
.1. MkrobEal Growth: I'he definition of gr()\N th, the rnarhema Heal expression lot growth/ the grm",th cun v -e. the measurement of grown, and grDwth yi l ·1;:;; svnchronous grrn·v t h: con tinuo U cu I tu r~; grow tlL a~ (i Hect~d by en v iron rnvn ta l Ia ctnr::; like temperature, ilci dity, alkalmi ty, wa ter availabill ty a net oxygen; culture collccticn
and rna in tt.!nElnc~ ~1f cult II res,
c" Overview of Ha:_.;iL :.\h::mbnl1srn ano yricrobial Nu~tjtlon
6. Metabol ic Di vcr'i.ity among MLcn)organ Isms : Photosvnthesis i n microorga ni~II1~; role llf chloropnylls, (MU enoids and phvcobilins: calvin cycle; chemolithorophv: hydrogen " iron - ni tdtE! - uxidiz:lng bacteria: rti trate and sulfate red uction, meHl<'Lnogel"lesis and ece+cgenesis: fer rnentaf 01 S - d i VE'I'Rit ' ::.;y n troph y, 'r6I!;'· If a noxic dccorn pusitiuns: ni t.rog~rr metabolism; nitrogen fJXi:"lli(ln~ h ydrocarbon trertsformation.
7 Prokaryotic div ersily
Bact~da~· Purple and ·g-ret:ln bacteria, cyanobacteria: hornoacctogenicbarturia: ~!idin.g ~Ind sheathed bacteria; pseudomonades: lactic and propionic add bacteria: pnduspuresA formmg rods and cocci; mycobacteria: rickettsias. eli lamydias and rnvcoplosma,
Archae .• l : Arr haea ,1$ the earHest lii\~' farms; halophiles: mE'~hcmogcns; hypcrtherrn nrhi lie archaea ~ therrnoplasma,
Eukarya : Algae, fungi, slime melds and f) '\lto ZO<l;.
Viruses : Bacterial, plant, animal and tumour virus. s: Th: discov '1"1', classificatron and structure of viruses: lysogeny; DNA viruses; posit1.vE.' strand, negative strand, and double sh·imdl~.d Rf\"A viruses; repllcation; examples of herpes, pox; adunovirusex, retrovi ruses, viroids and prions ..
-t). r .... Iscrobial Diseases : Disease reservoirs: epiderm Iogtcal terminologies; infe.cti.OllS tiseaSlt' transrn iss] on; rCS"Fi ra tory i l"I f\:~c~i ens caused by bacteria and viruses: tnberr u losis: soX'uA'lf transmitted diseases including AIDS/ diseases transmitted by animals (rabies, plague), insects and ticks (rickettsias/ lyme disease, malaria), food and water borne .diseases: public health and water qualtty: pathogenic fmlgi, emerging and resurgent i 1'1 Iectious diseases.
9, Host-Parasite Relationships :. Norma] rnicroflora o skin, oral cavity; Kastrornteslioa!
I. act; entry 0 t pa tho-gens in to th~ host; colonization and tactors pred i ~ posed to Infecrions: types of to·xins (Exo, Enda-Entero) and their structure: mode of acuons: vi rulence and pathogenesis.
10. Prokaryofic 'Celis; Structure-I unction: ern walls of eubacteria (peptidoglycan) and re I a ted molecules. ou ter membrane or .gra m-negative bacteria: cc 11 " v <1.1 J and cell membrane svnthesis; flacella and motility; cell inclusions like endospores. V,lH vesicles.
.. l...,,, ~ o,";l •
11" Chemotherapy / Antibiotics: Antimicrobial agents: sulfa drLi.g5; a ntibi otics: penici Il ins
ft nd cep halosporins: broad -spectrum antibiotics: antibm tics Ii-om pro karvotes: anHfungal antibiotics: mode DfiKhbn; .l"E'sistanQ:? l() a nnbjo~ lcs:
L2- Gt'n~B,. mutation and mutagenesis : UV and chemical mutagens: types Df mutations: ames test fur nUlt;lgene:'l!s; methods of g~n.E'Hc E1cl1.3l.ysi:j;_.
lJ. [)actf'rial Gl"netic Svstem . Transjormatton, cunjugation, transduction. recombination, f!L·l:smid.~ <1n,d trLH1:5pDsing" bacterial genetics rnap witlr uderfc'nq h~ E. c(,)ii.
l-l. ViH1St;:)1 and their G:;'neli( System i I)h'~lgt' and ds me cvcle: RJ'\JA phaf;1;t's.; RNA viruses:
D,:' t ro v iru 56_
lj_ G[?nelk Sy5t~ms nf ,"\'eELo;L ,md N;el,J.ro~!~qr<i.'l
l ()_ ExtTw:;hrmw")Mlmal Inheritance
1
P!'t>parat"i:r~n o·r liquid and solkl media for grm .... ·th 01 fnkml)rg,'H"Iisms
lsela tion and mairireuance of l·nganjs~rl~ by plaUng, <;[reil ki ng and serial d ll u linn. r1wthuJ"s, ~~anb. M'1d stab cultures, ~tordge ~d mltruurgluU~rrt::l
lP,ohrl ion of rnlfeC'u I.lurf's r rom soil a ndwarer.
('rl1wth; srowtn curve: rncasu r;em~nt .. of bacter ial pot' LLl~ tl on by lu rbidornet rv (1 n~'l serlal ollutton methods, EHect of temperature. plI, carbon and nitrog(m. sources on
gj~n.".'th.,
y.. Vlic..:n ... :-scopi( examination oJ bacteria, yeast and molds and stu.dy of l~rg,tn:bms by gram stain, acid f~,.,t stain and staining for s.pOrE'5~.
-1.
{~, Study of mutations by ames test,
7. /\SS;>,V 01- antibiotics and demonstration of antibiotic t"esiSW.ITCC.
.. .
] 0, Trartsduction.
L Geri!€'if"a~ Mic,ohiology, 'Shlinil"if, R-Y<,ir~grilhinn, 1-1.·, \VheE'lis, M.L, <1fUJ F\;1inter,P.P, The McMillllJl Fjr~s-~ Ltd,
2_ Brock Biology of Miuoo-rg4lnisrns. M.~digan, M._ r.. Maftinko, jJl/l- and Parker. 1.
Prentice- Hall,
1. Mlrr(lbioh~g:.y, Pd(:~z.ar, rVIJ_, Jr. Chan, E.t;:2i." and Kn;,lg; N.i~_. Teta-McGraw-Hlll.
~, MicrobIal Genetics, Maloy. S-R., Cronan. _T.Lh-. and Friefelder. D. Junes and B.uHeH Pu b] i5h[:;1":5,
5... Microbiology, A Laboratory Manual, Cappuccino, _l.G" and S'U::'ITnan, N,. Addison \1I,i'eslev.
"" ."
6- Microbiological Applications (A Laboratory Manual in General Microbiology), L:'ens;on,.
H.LW,(.B., y...rm C Brown Publishers.
4 Computer Applications and Biostatistics
I, Brid descrrption and tabulation of data and Lts gl<'1pbical representation.
2. Measure nf centra] tendency and d ispersion: mean. median, mode, ran g,e,. starida rd devia ti on; variance, Idea of tVIHl t YPI2S of l'rror:;. and level of signlficance, tests of significance (F 'and l test): chi-square te~i"~,
J. Simple Iinear regression and correlation.
-i, Introd ucti m oj dIgital compu ters: (:)1"g<1 i1 izarions: low-level and high-level bn;guag.es,
the binary number system.
S . Flow charts and programming techniques.
p, Introduction to programming ln Q basi: and C.
7 introduction to data structures and d21ii'lbfl.se,· concepts, introduction to internet and its applications,
8, Introduction to MS·offi(E' SOitWMC'1 covering , .. vord processing, spreadsheets and presentatiou software.
q_ In roduction to Harvard ~raphic5/5igma plotter.
1 u, Cornpu ter-oriented statistical techniques: Frequency table l'1f single discrete variable.
Bubble sort, computation of mean, variance and standard deviation; Hest correlation (:o~ffici,en~;
11_ Bio-mformatics and bio-technology
5 Laboratory - ~
2nd Semester
Theorv
1. lntrod uction to Molecular Biology and Cenctics
2. DNA Repfication: Prokaryotic and Eukaryotir DNA replication, mechanics of DNA replication, enzymes ·.and accessory proteins involved in DNA. replication.
1. DNA repair and recornbination
.:!:, Ttaoscription: Prokaryotlr transcriptron, Eukaryotlc transcription, RN A pol ymel'3se.
Generl'll arid specific transcription fa.dors, reguJ,,'\tcry elements and mechanfsrns of
Bio"TECIiN'C)tod·Y· - -
. . .. -~. - ~ - .'. .
transcription re.g:ul ... )tiorHs/. transcriptional and post-transcriptional gx~ne s-ilencinp"
5, J\llnd"ific£i tions in k.r\ A : ,r:;,' - Cap (onnat.:iotl, lrjll1~Gi'irtlon tE'r'mif'!;:lth:)r'l, Y -end PN(!..'$.sjo~ and polyiHJenyl\'ltiorti splicing, editing, nuclear export of mR:\1A, rnRNA ~,[ability,
6, Tra nslali on; ProkiH)' utk Jnd E u karyotic trans la tion, tru} tr anslatian machinery, mccharusrns uf in i tiation, ~16ngati.on and terrninatu m, tegul.ation ·(If translation, co- and post-Iranslation rnodHicu lions of prnteins,
7, O~lcog~n~5 ;;Ind Tumnur Suppressor G~J:'1es : Virul and cellularonccgenes, tumour StlppreSfjm~ genes from huruans, structure, function and mechanism ot action of pEB and p5J tu mou r suppre::}svr prM8j ns.
:), AnEs.eni'lse O'lnd Ribozyme Technology : Molecular mechanism uf antisense molecules, i nhibiticn ~$f splicing, . pelynderiylatien and translation, disru pHon of I\}'J A ~frw::tl.i re and .ell ~)r ing! bl ochern istry or rib07.}'roe; ham merheed. ho repl n ·'H1.d other rlbozy mes, ~trM~gies f[)J d~sib'1ling rjiboi:yrn~'l:;~ applicaticns ~jf <·U1ti~L'n!:i;(2· <H'l.Q .r.iil:.1()zpne technologies:
q Hamoiogcua Rebonll.biHaNcm : Holidav junction, gent' lJrgE'~tng, gf;nt! disru pttpn. FLP!
FRT and Crt'! Lox combanation. R.ecA and other reeombmases.
I (}. Mappingof Gc.nmTH~:t;{:'nctlc rmd physical nl<lp.. s , physica I m:Jppin~ and map-based clon ii'lg, thoire of ma pp i ng population, simple sequence repeat loci ,. southern and fLllcrn:'5Cl'net;'in ~ttu hybridization for g~norhl' 2lnalysis,·chyomD~'Q::-;rne microdissection and microcloning, !nn·lpcul'H rnar kers in g,enom·t' analysis: it rt. p. RAPD <tnd AFlP .'In"i'lly$is~ molecular markers linked tu ~1i:5,e(lS~ resistance gCUl'S, Appliqtion of RFl.P ill fo n:'nsk_, d:iseaseprpgn osia, gene tic counselli n~, fJed i gn~! va ristal etc:". All i rn a I. trafhtKrI,g 21l1d pOi3-rh i ng.~. gE'roip.k'L·srn rna i nten"O'lrlQ't" t .. :n::cifl1..'lTny i'lnJ biodiversity,
I I. , r.;enCln~e 'Sequencing ~: Cel1CHne s~1es. org;~ndle _genumes, genon'lic T ibraries, YACi SAC libraries, ~tr~lt(:sit.'s fur S'~qucn~ing genQ]]~, packag .. i.llg, trarrsections and fecfwPfY of clones. (11"J'pH(Cltion of sequencE' information fer identification of defective genes.
1; Isolatton of ge-nnm~c IjNA
2. Southe.rn b~()tting
--Ie , lsol arion Aif Rl\ A
5.. lsole+ion. of poly A +RNA
f1, Northern blotting
7, Prepa ration of probes
cL. ln vitro tTan,s(l'ip lion
q h'l vitro translatlon
10, Metabolic labelling bf proteins and irnmunopreeipitatton
Book~
1, MoleculM Clnni[l;';; A Laboratory tv1.~uiJ.ual, J, Sambrook, E.F., Frils(h 'and T. \Ol~ni;~th Cold Spring ilarhor laboratory Press, Ne.w York, 200CL
2, lntrodurtion h' Practical Molecular I~io!ogy, I:I.D. Dabre. [ohn v\tile.y &; Sons Ltd., [\,Ip\· York, 1 Y9S,
J. Molecular Bi(dugy l.abiax, T,A. Brown (ed,), [$100.; Scienfific Publishers Ltd., Oxford 1991.
~~. Molecular Hlology of the Gene (4th edition), J,D, \ .... /al (_)I1, ·N,ll. Hopkins. ,T.W, R'lbl;'rtb.
J.A. Steitz and A.M. Wl'iner, the l3,enj,lmhl]/~':umn1ings Pub. Co .. lnc.. Califnrrna .. lYt:?
:::; . 1v1 D lecu l~" r Cell Biology (2n d Edi non b J. Darnc I L .H, Lod i sh and D. !3 a ~ Li more, Sc i en l if j ~ American B~)ok;; inc, USA 19Y4.
(-i, Molecular Biology of the CI:'H (2nd edition), EL Alberts, U. K·nly~ J, lewis, M. Raff, K Roberts, and J.D. \'Vatson .. Carland Publishill.g lnc.. . ~w York, lQQ·1-,
7. ';~~ne VI (6th pditl .. m), BC'llja:min Lewin, Ch:rmd Univ crsity r)rI:'S~, C,t., 1~9fl.
B. Molecule r Biotog}' and 8iotedmoIogy, A Comprehensive Desk. Rder-enosl RA. M(~yerf~~ (t·e!..), VCH Publishers lnc, N{yl.v York, 19·95,
Suggested Reading
7 Macromolecules & basic Enzymology
Theory
1., Macrrirnolecu les and supra molecular' assemblies ' Types o! macromolecules in blolczical svsterns. molecular a<JSE'nl h I ies ~ i kc ruern br;3.nes, ribusomes, extraccl lular
, J
matrix,
2..Physip=i1 techniques in protein, nucleic acids and polysaccharide structural analysis (LTV. IK NM 1(, LASE'I\:, Raman sr<;!ct1·~'tiWpy, rv1 ASS ~p~.ctr()scopy., tluurescence spectroscopy). Di Hercntia I r alurimctr y, X-ri'lY crystallographv. ultra centrii ugation, Electron crvo rnicroscopy: SGHLn.inCJ ~L1tmt~liing mien .'>(01))"
3, Seq LJCBci rig ol protei ns and n u(,lelc <Kids .
. 1. Protcin-p f't1 tE;' 1 n and prote] n-ligand In ti;>r9~tions,physic,31 and chemical. methods for stud: .
5. Conformational properties ~lf polynucleotldes and polysaccharidcs - secondary and tertia ry structural features and their analysis - theoretical and experimental: p rotein tolding - biophysical and 0;11 ular asp cts,
h. Protein a nd nucleic acid data bases: structural compariscn at 'c'condarv and tertia toy levels.
-
7. Ettzyme cata l ysrs i n ~ol ution- kinetics and 'Iherrn9d vnamic .<:U1i1lysif" effe(t~ of o~g<IIli.{:
solvents on enzyme catalysls ,mJ structural cons quence .
e. Ph y. !i.l.C<I] and eh E' rni cal, it~ ethods for Irnm o bit iza 11 on ·ut small and macrom L11 ('l'1.d 0:5-.
l). Glyc-() and ! i P If)fl )tL'i n - structure and h .. mction.
1 (L Org;'fl1ihlti()11 pf macromolecular complexes - .chrnrnafin md r ibosernes, pro+ein dena tu HI tion.
u.
I L Nucleic acid hybridization - structural analysis and b'iolngic.11 studies.
12. Ribozyrnes and. Catalytic antibodies - Functionalproteins structure "'-111\,1 drug; turgets (enzymes arid n:::ceptDr'iJ,
14.
Cum r uter A.ided drug ch:signll'lg, cornpu ~ah(rn,rl techniques ! n structurel lilVllysis.; nan ().P;l rt icl t' S".
Allosteric cnzvtues, mnemonlcal enzvmes, kin tics of t:'fI}:':vrne inhibitors.
- ~. l
P r ac ti c <L 1 s
3.
-L
5 ..
-
/.
K. 9. w. 11.
1.
Elcctrophon . .s.i::; of proteins - nati \'~ and und I'~d ·oat-tlring conditions. f\~- 0 nd C - terminal analysis of proteins.
Peptide mapping.
Separatron techniques (HFl.C, CPC, FPLCL ion exchange. gel filtration, affinity '11 romatcgrap hy.
Chemica [ mud ification of protei ns. Enzyme ; purification and Hn~j(_ analysis.
Hydrodynamic prop~rtu.' .. - measurement and applications. rvh:,thod!5 tor immobilization 01 enzymes.
Techuiques for an alysis- (If sccondarv, tertia ry ,:ln~! qW\ ternary structures .of proteins. Electrophoresis of DJ\'i\ - lint:'ar, .ircular and super coiled.
Protein - ON A in teract I ms,
6 ..
"[2,
~ urleir acld hybrid izaticn,
. .
Is.
Deterrn inution of Tm of nucleic acid.
I-t_ Enzyme isolation horn aricus tis . sues, prer ip itafion methods for purification ~l1.l1J er:i~yl'(fe proteins.
Books
1. , Essen tials of Moh .. xular BiologYI David Friefilder. [ones and lJanl.f~t I'ublications.
2. Pre tuins-Structu re a nd Mll~ ecu i ar Pr6p,ert~esr T~~: C rl;;"i g hton, \-\'H Freern a n a n d Cornpa ny.
~" Genes Vll, B. Lewin, Oxford Uni·vefsity Press,
I
-Y.
Introduction to l:!rQtein. tructure, C. Bri'lnden and .I. Toozc, Carlar d Publishtng, Nev York,
EIl(·Y·t.: lo ped j,tI()f M D]!.::"cuar Bf~..llug) I J. Kerid I'PW, Blackwell Scion rifi; r u bl i ca l iDn~ Oxford,
7,
I'hv-viral Chemistrv of Macromolecules. Tanford. c., John Wilt,!,' and Sons.
,. ,
Irrtroduction tl~ Kinphy:;ical Chemisrry, R.B. Marlin, McGraw Hilt :-Jl.:wYork.
Biopbvslcal Chemistrv, Cc(1 ntor, 'ltV _H, l-recman,
Proteu Structur . Max Peruz,
H...
\I,
B Bjo!ogy of the nn mune system
Theory
I . In trod uction
_ l)hvlpgl'H\' ofimmune system
_" ~ - .",
2.
_ r nnalc and acquirud i rnrn uni t).
_ Clonal IV tun' of irnrmme rc~pon~e
('Jrgan tzaliou and structure of Iym i)hold ~lrgims Nature and bluLu}.;y of <Inti r:,ens and superantigens Antibo: y . tructure and function
Antigon-antibod y in teractions
Maj or hi stocumpa tibi 1 it Y (Dfnp Ie>;
BeR & TCI~ aeneration of di'l .. crsitv
;:, .
J-t, 5,
6:
7
Cornp lern 'M "y:!ott'lri
Ct'lb () the immune system: Hematopoiesis and differentiation, lymphocyte tl\~mC"k[n~ B~]ympbucyte$1 T -lvm phocytes, ruacrophagcs, dendritic (db, natural killer i"1l1
lymphokins activated killer {: •. .ll». osinopht], neutrophils r: md rna t ('eli.;; ..
Rc~uhtion ·ofi mill uo(! re..":-poDse.
_ Antizen PJ·pces.sing and presentation, g~nerahon of humoral .'lnd cell. mediate Immune [l'spouses.
~ Activation yt B , nd T _ lymphocytes Cytukine-, .'lnd their role in immune rEgul.~hon _ T~(€.'n regulation. fVIHC restriction
_ Irnmunologlcal toleranc :
II. Cell - rnetliutcd cytotoxicity : Mechanism of T eel! arid NK cell Medratcd lysi '~IlHbt H .. i y-depandcnt cell-mediated cy totoxicity I macrophage Il"lt'd i ated cy Lotnxici l. y
g-
9.
L2. Hypersensitivuy
I J. Autoimmunity
-
4.
M~Sc. GENERAL BIOTECH·NOLOGY
14. Transplantation
J h. Tu mOUI irrHnlHlulu~y
1..7 _ 1'\J IJS -and LrUwr irn rnunorleficiencies
PI a. c ti GI .. ls
IJloud Him prk~FM.r'ati~)n and identification 01' cells Lymphoid orgi'lD14 and thei rmicroscoptc prguniz,at.ion 1 mrru i ni.ca i ion, t'ul~eninn tH "'·E-'flJ m
.~ ..
Double (J-l ffllsion ,l nd inrrnunu-e It'{Jrl~rh!,ir-~'s!~ Radial irnrnuno dirfu\lml
rmitication or IgG trom sern n~
Sepal'ati()tl of mononuclear cells by FiQ11 l-Hypaquc
0)"11-,-\ 1t1d uced p·,oIHer8t-i un d th yrnocytes (by r\-HT metb.ltd) W("stE.·nl-bl~1tting
6,
tt_
10. n,
ELIS~A.
Hapten umjug~tion <utd. qU<lntlzdti(}J'1
1 m m unodiagrtostics (d0J1HlI.s~[a tion using C()11lU1E.\r;;:ial kits)
1 ~;
Books
I,
Kuhy Irnm lIn~)!(\~y I -tth ,~d_j_tior1:,. R. ,i-\. G!)ldsby, Thomas J. Kindt, Barbara A n~h~jinl' (Freeman) .
[In fn.\.ln.ofogy! A ·ShD'rt C(m"5E:'~ 4th ed ition, EJ] Bcnjarn in, R~dttl!td C oico. .Gcp.ffn .. ·y Sunshine (Wi lev-Lissl,
FUfW10'1.l1.1{C'ntals of Imrnunolcjrv iWiI J ium P('l'U 1-
':'.).f
9 Biophysical chern lstrv
A... .~ nteracti ens LJil m:ologi cal Syste.n'is
1_ J 11 tra and j nter- molecul ar forces, electrostatie i nteractions an d hyd.rogcn bond infj; in ter:i.dl on l-1;
\/ andcr Wa.al.~ ·<;lm.i I ~yd rophobic interactions lJi:;;u.l phide bridgE::t'i
-
-1,
B,
Stnu::hJ re a,f Prete ins
ton formationa 1 pn }ptJft it~S n r polypeptides
Pnmarv and scconelurv srructuro a-helix. b-shoet structures etc,
1
4_
T E'1-ti;;J ry ~nd q. u aterna ry structure StrllCtur<l1 f~dtUI·e~ of membrane pro'lE'in~ C on] u gated and rnelal-bindiru; proteins
S~\hmdLl ry M'Id rl'rtiMy <tru ctu r'~; p red iet ion or protein conformation
"
Multiple Equlllbrium
Ti ti:~ tion uF prnh:m~ ttl l''i aluate net and l~ )"tal ch.:i.r:~l\ ~C<'It~~h,Hd and hill pk,h
FuldiI~g-ljnIOkl inpt'quilihrium and denaturation or proteins
Effect (iff temperature !1n~J ~~lht"111 condition on Lb.\." thcrmodvnamics ~)f protein foldin u nl 01 di nt) t;"llui il h i'j urn
Kinetics of protein ~old""lg
Technlques r(')!" the study of Macromolecular Structure
J-L
.J,
D,.
1 ;
I' "I '
LI rr 0(· J ntr't II.g;~ r1O])
molecular weights
'Ii i';'C0Silr
: S~>d 1 ruen l J Li em velocity and cqul libr i u m, d (, re rrn i n~ t ion
,
3. Micro c(!I]urirneter
l _ eire u 1M Dichroism sp ectro:;>("l'lp y
~ U V, v i 0; ~ b If' a nc1 n LI f'J rcsren ce :->p~~ tw~opy
6. X-Ray diffraction
7 _ 'J urlear magnetic resonance {Nh·1R)
10 Elective paper
11 Laboratory-'II
3rd Semester
12 Animal Biotechnology
Theory
1" Structure and organizaiion tif Animal ell
2: _ Equiprrtents aml rna teria ls for an irna I cell cultu rc technology
-
Pri f!1dTV and 'i'stabHsllell cd1 1 in~" cult ures
111 t w~1 UI;:tiuli to tile ba 1 anced ~[ll t solut ions .:md sjm p'lf' gr owth medi urn, ;.\ bnt,j d i:;cLlS5JOn on Ihc .. hernica I, ph p:;ical .md ruetabolic tu nctions ot d ifk:rent constituen ts of cu llurc rued ill m .. Role o] carbon dioxide, Hol.e I'lf C;Cn.HH and supplements,
Sen"] rn .md pmtl'; n-frcc ddi rwd media ,HId their .JF'plicath)ns
:\1l';15ufeT'nent 0 F v iabUit) and I..'yt(~t{lxicity
n i{rl(~gy and charactcriza tum \")f cultured eel ls. rnl'i..~o;UTiIlg parameters. Df :e;!'m,vlh, H<l~;j( tL'chnjqlH~,'i ulrnar'pmalian (eO culture iii vitro, dis(lggregation of tissue 'and r1 ri marv culture: maintenance of cell culture; cell s.epara tion
SGlling-up of animal cell (,:ulturt'
1_ 't-:1l ;-:0\ nchronizarion
, .
1, Cdl cloning: and micromanipulation
~dl lrnns'I"OlITlation
3; ,1\ pplk.'ltioTi o[ LIT) imai (f'11 culture
·L Stern eel] cultures, t"rnbryonk stem ~I!:S and their 2~ppliC'Mjons
5 ,Cl.>ll culture hasc'd V<lcc-i nes
f." Sornatie cell .genencs
7, Ort;,em and hisot)"pic .cultures
R_. Mt='<'1suwlncnt or cell death
lJ Ap("lph "Sis
~O, Three dimensional culture .Hld tissue t'I'IfineE'ring
Pract icals
L, Prvparation ()f tissue culture rned ium and membrane mCD""iltion
2_ Preparation of single cell suspension from spleen and thymus
-4-.
Cell ,,:-ountirtf; LInd c~11 viability
MlldoI ·h.:tge mnnQbyer Ircm PEC, and measurement of P 1101 gocy tic activity Trvpslruzation (If monolayer and subculturing
H. Rol.e (lq serum in cell culture
,-},
Preparation ot mctaphage ChID ru 5N1U5 from cultured cells
Isolation nf U~\I A and demonstration of apoptosis of Dro..JA laddering MTI assay for (ell vi.or.bility and growth
Ce'll susion with .PEG
10. IL
'2.
.,
'r-DNA, T-PNA
cells.
. \,VtL
:.., Be]'!
nd C .
Books
1. Culture of Animal ... Cells 3,d Edition, R. Ian l-reshney, \-Viley-lEss
2.. Animal CE'!J ~ Culture - Prartical approach, ed., j Uh11 R.W. Masters, Oxford
3_ Cell GroV'I' hand Division: A Practical Apprc ach, ed., R. BaSE-ga, [RL Press.
+. Cell Culture Lab Fax, eds., M.Buthn and M. Oawscm}B].us Scienti fie [~ubhr.atiDns Ltd" Oxford,
5. Animal Cell Culture.Tecbniques, cds.. Martin Clynes, Springer-
fl_ Iethods in Cell Biology; vel. 57, Animal Cell Culture Methods, eds., lenni P. ,V1]F:lth~1'
a nd David J32U"nt"~.' A ... 'ildt:mic Press,
13 Genetic Engineering
Theory
1 . Scope of Genetic Engineering
2. M i t@.stone~ in g<:netic engi neering: Isolatlon ofenzymes, DNA sequencing, synthesis and mutation, detection and separation, cloni . g, gene expression, cloning and p3 tenting of life forms, gen~ric ~ngineerir\g guidelines.
3. Molecular tools dna their appli{'i.il'tions.: Restrictior; enzymes, modification enzyme~, D. A and Rl\ll\ marker«.
-1. Nucleic acid pirrificatton. yield analysis
? Nucleic add amplification and its applications
6. Gene cloning vectors: Plasrrrlds. bacte rlophages, phagern l cis, ,n.,;'rnid~, arti fi cia ~ chromosomes
7. RestridkHl mapping of DNA h,agments ancimapCclOstr'UctiDn, nucleic acid ~l~ quenc Lng.
8. cD\JA s 'nthesis and cloning : rrt.RNA enrichment, reverse transcription, DNA primers, linkers, ad aptors (mel the] r chemical synthesis, Library construction a nd screening
9.. Al terriaf \'e strategies of g.ene clon ing: Cloning in te racti ng gt.~nes - two-and-th ree hybrid systems .. donlT'rg d i fferen:t:i ally expressed gen~:;. Nucleic add rnicroarray anays..
i O. Site-directed mutagenesis and protein engineering
I I . Hem: to .study genE' regulation? DN ,\ transectiorts. northern blot, primer extension, S'i mapping, Rnase protection assay, Reporter aS53Y.!:,·.
IL i=.xpresslOn strategies for heterologous g·enes ; Vector. engirre ring and codon <-\pti rni zation, host engine€ ri ng, in vitro transcription and trans lation, expressIon in bacteria, expression in yeast, expression in insects and insect cells. expression in mammalian cells, expression iII plants.
13, Processing of T.ecombin;,u1t proteins: Purification and refuldiIlg~ characterizntion of recombinant protei ns, stabilization of proteins.
14, Ph<lgf' dk:;p]i.l:_ir
15-. T-DNA [mel Transposon ta~gLng'; RoJ~, of gerlt' tag~ing in gene analysis, T-UNA and transpo~(m til~ging, i deretifica tion and isolaricn of genes lhruugh T -DNA Or Ir,HI5J"IQ~O;P,
1 e, Transgenic and gene knockout technologies ; T<~rgt'tcd -gene repLOIC men l, chromosome engineeri fig.
17, Ct"nl' therapy: Vector en:gltleerjng. Str'aLgi s of ~.ene delivery, gent· replacerne nt / .a Ll~meli tation, ge·n correction, gene editing, g~ne reg.u lation and silencing.
J <5, Isolation ufplasmids
Practicals
1. Hr:Ij:--kori..,) ~:ll lture nnd [I ntibiotic ·d~ct.i~ill media .. Preparariun of competent cells.
2. ~ rolation of plasmid DI\:1\
3_ (j'uantif:alion ot nucleic 'Kith
4 - /'l.g,;trbSe gel electrophoresis and restriction mapping of- ON l\
s_ Construcf 1;)11 of restriction map of plasmic ON.-\
(1 .cluning ~n plasmid / phagem tel vectors
7 _ Pn."'P21 r<il Hon oj' 51 ngle stringed DNA template
H_ DNA sequencing
9. C;~ne expression .in !:'._.o)lj and arMly~is ot geI'l~ product
io. FCT~
11, R~}--iOItl'l- grni."..' assay (Ctls/CL\T/~-CAI.)
Hooks
1, Molecular Cloning: A Laboratorv Manual, J. Sambrook, E .. F, Pritsch and T_ _\1anlati:'i_ Cold. Spring Harber Laboratory Press, ~.l",\' York, 2:UGO.
2- DNA Cloning: A Prac+ical Approach, D.l'vL Glover and B.D. I lames. IRL Press, Oxford, 1995_
:::L Molarularand Cellular Methods in Biology and Medicine, P.Il~a!,.lfrn;;l:n, w. V\"u, D.
Kim and L], Cseke. CRC Press, Florida. 19.95.
-1. Methods in Fnzyruulogy, Guide to Molecular Cloning Techniques, vol. .152, SJ .. ,l~e:rger and t\.R. Kimmel, Academic Press r nc., San Diego, 1.996
::r. Methods in l~n7ymo]O~YI vo]. 185"-Il;'ne Expression Teehnology. O.V, Goedd 'i, Academic Press Inc. San Diego, 1'-)90
6. DNA Science : A First Course fn Re-(~ mbinant Technology, O;A Mickless and ·G_.A_ Freyer, Cold Spring Harbor Laboratorv Press, I\c.:'l ..... York,' 1 SJ9(1
'.
r . ·M.Sc.- ,'GENERAL"BIOTECHNOLOGY .1,:
7. Mpl~J .. 'uJ.:lf' HiqtechI'lulogy I 2nd l'd ition, s.!), PrlI)tr::o~~~ 13J.~.(k well Scientific l'ublishers.
Ox ford, 1-9'9-+,.
8. Ml lesrones in Fl·iotcchnul 015)', Ci.f15'SK Papers' un GenE,tic EngJ.net'trngJ J./i.., Davies M'ld V'.l,S, RCZ)likoffi Butt~) .. wcrth-Hcincmarm, Ui}stollri 1991,
IJ Ro u Le Maps in Cent~ rechnology. M..l~ .. \:'\/ alker ,;'l rid R. RQpley, HLJ(bvt!11 SciencE' I JeL. 0>-: F,ard"" 1997 ..
10, C t~ne-tic Eng i nel2 rlnfr An In trod ur l i l)ll W Ct'nro Anal ys b <H'Id E,}.. r loi ta lion i t1 Eukarvotes, S .. M, Kingsrnan and A I, Kingsrnan, Blackwell Sctcntifsc 1 .. "ubli.Ul tions. Ox toi'd, ] q98"
1 4 Pia nt Biotech nolo'9Y
rhl'ory
1 .. Conventinnal plan l .b.t'f'pdj ng
2. lntroductirm 1-0 C"dl <1ml tissue <:.;uItur~, tissue culture <lr. ~l technique t{l~;i""u .. Jun' ru ive I.
plants and hybrids ..
J. Tissue culture media {COIT'lq:wsHiOl,l arid prcparution).
-I. lriitiation .arid mai nteuartec QI[ LaBus and suspension rultu re: si ngl~~ cd I clones.
:). Org.:ll"lo~f'nf's,if:; ~mna hc ,embryog.f~nE'~",Js.;. ira nsj'~t' and e.~Lnrbtlsh ment l)I' Wh0h~ plants (n soil,
(~, Slroot-tip cui tu H:-:. RiJpiq ·dt)I1J11 pmpagation and p~:~)duction of virus-free plants;
7, Fmbryo cultu n.' and tOrn b rye. rescue ..
b, Protoplast isolation, culture emu fusion; selection nf hybrid C'~U5 and regeneration pi hyb ri(l pri'1nts~ sy r(lTiH:'trit' rl11d .,l::.ym metric hyl:n~L..h"h ybrich, B"l()[(,~n~fGrm.bHoll,
t), i\nt.i.lcr, pollen and .m'ary culture for prod uctlon of haploid plants and homD.liygHLLs I ines.
1 O. Cryc.)preben~<Jti on, ~Inw growth and D["-.,~ Abank.ing for gCTl11 plasm conservntion.
1 l.. Plant 'I rartsformation ~eehnf)J()gy ; The basis of tumour forruation, ha'try root, featut'~s of T I '~1nd R1 plasrnids, rnechanisnt= l11 DNA ti~lrl:5fer~ rql~ or virulence genes, useof 11 and [\1 as vectors, binary vectors, uSC uf.355 and other promoters, gl\Ildic: markers U:"~ of r~p()['~er St'nf'R, re!)(}tt{'f f'jenf' wiLh il'lt.ron.'\,L.:ifii;;' of smrfojdau~cbmenl !'~gjun.," meth/l~b ofnuclear tran.,hYrJn<itiqn, viral vectors and their applications, rrrultiple gene [mn.,fE.<fSiVedodes-s or d 11'E'c[IJN A transfer, partlcie bomb .• rrdment, c]octropDral'ion .. microi njl'diu!} transformation ~}f rnDt'lm:ut~, Transgene stability ~mJ geut sjlt~ntint\:, In planta h'£I n<;fmTll f,I tion,
r 2... Apfll il~AlIun uf pbnt tr~nsFOrTrtMi\)n for rn;Jdlh~b\l]ty and performance pr-vnuJt&r trJlpping, acti vation ~aggill;g" Herbicide resistance, phQ~phuj notluicin, g:1 rphOS~lt(' I' sufenvl UH:'<'1, atraziue, insect resistance, Bt, .g:en(~sl non-Bt like prntease inhibitors,
alpha amylase mhibitor. virus re 'i:,,;tanct',~)at 'rnt~jn mediated, nucleocapsid geot!, dise •• se l'e5i:;>L:111l:~, chitinasc, '1- i b"t,~ gJ WJ nase, RIP antifungal protein:" t h ioni nes, P'R proteins, nematode I't sistanre abiotic stress post-harvest Iossvs; kn,t; sh~lf life L ,f fill it" and fl0\Jvers, use of ACe svnthase, pol v~ ~illannt.l1ilse, Ace oxidase, male sterile lines,
II - ~ ~ _.
bar and barriase ~y. [em;", carbohyd r .. ;lti2' compos: tion and sturage: A DP' glucose
pyrophosphatase. terminator gpne technology.
1::;, Chloroplas! transformarion; r\ch,.:antage:;., vectors, success with tobacco and potato.
1 .1. Meta belie 'l'nginedi r1g and ind ustrial products ;' PI~1n l secondary metabolites, control r,nechani:::1TIS and mani-pulation !'l{ phcnylprnpanoid pil hway, shiJ,.imflt~ pathway: alka loi Lis, int! ustrial enzymes, biodegradable plastlrs,
p(ll vhyd t'oxybutyr<lt(:.·, thera pt:~u tit" p roteins, l.y..,()s(~rn~ll ~~n..!.y mes. ?I n Lil,ndi P:;;', edible v accines, pun I iG~ (ion stt'<11i:.e-gics, oleosin P" rti tiorung technology.
15_ Molecular marker-aided bweding: RFLP maps, linkage ~1nalysi!"o, [{APD markers, STS, rnicro-atellitcs. SCAR (sequence characterized amplified regions). sseI' (single strand conformational pol y morphism), AFL P, QTL .. map-based "clnning-:, molecular marker,1 S-S L sled s E' lee ti on.
lo_ Arid and senti-arid plant biotL'l:hnoiug_ .
'7 CrE't:~n house and Crcen-horne ted1~1lI1.()gy,
Pracricals
2, S~rfi'i~t' steri l i.7.<1 tion
3- (-1r~'Hl. culture
4- Callus l"lr(lp~gation, mganugt'tL.t.~si$, ~I'an:,;;fe!' of plants to soil
~_ Prt'ltf:ipkist isolation and (u l ture
6, Anther cul ru re, production of haploids
7, Cytological examinar-ion of l'egeneIatcd plants
so. Agiub.1Ctel'ilH'Il cultun .. ~, <;.('h~ttto.n M transformants, reporter ~e(!'~ (G US) assays,
9_ Developing RfLP and RAPD nlFlp"
Books
]. [, Hamrnound, P. t\kCarvcy dud 'I,/.Yu::;il)(l\-, eds. Plant Di:l~h;.chn(jlogy; Springer V' .!' 1 ag., 2000
) T-J Fu" C. Si rtgh" arLd\V.R. Curtis, eds.. Plant Cell and Tissue l...~U 1 t u t'e for the [~roduchon of Feud I ngre-d ients, Kluwer Academic / Plen 1.H11Pl'f:lSS} 1999,
3. n5, Chawla, Biotecb.ntdugy in Crop lrnprovcmcnt, International 60rk Distributing Company r '199S_
BIOTECHNOLOGY
-
-L IL J- Henry, Practical Application or Plat1t Molecular BiolohY, Chauman dnJ. Hall 19~:l"7 ,
15 Student Sem inar
16 Project
4th Semester
17 Bioprocess Engincerjng & Technology
Theory
1, Introduction to biopr ) .ess t'r'tg:ine,ering
1. Bioreartors
.1. tsolation, pre~l'n" arion and maintenanc . of i nd ustrial microorganisms
.,[., Kinetic of microbial grm .... 1 th and death
.5, !Vh_~dia for lnd uetrlal fermentation
(-" Al r and media ster ilizati on
/, Types or ferr en t~1. tion proce. 5!:::5: Ana] ysis 0 f ba lch, ff'd -ba tch ant con tin uou: biurcactions, biotranst ormation, Stab: 1 j t).' of microbial reactors, ana lys: f, of m i xec microbia I popu la tions, specialized bioreactr TS (pulsed, fluidized,
photobioreactors ctc.j
K, Measurement arid control of btoprocess pi1.r.ameterS
4 Downstre ... J rn processi ng: lntrod uction, removal of m icrobial cells {lIle! sohd matter foam scpararion, precip i til tion, (oj ltratlon, centrifugation, .cell disruptions, liquid-liq L,J ir extraction. chromatography, membrane process, drying and crystallizatlon, etfluen rrearrncnt. DOC and COD treatments and disposal of effluents.
to. Enzyme and whole [I;'ll irnrnohilizaiio» and their industrial applications
11. Industrial produ cfion of chemicals. Alcohol (ethanol), acids k1tI']C, acetic a nr gILl.~:cmi(), solvents (gJycewt acetone, butanol), antibiotics (penicillin, streptomycin tetracycline}, aminoacids (lyscne, gl-u carnic add}. single' cell protein.
12, Use of microbes in miner-al beneficiation and oilrecovery
] J. lntroductlon to food technology
Elementary idea of (ann i ng and packl Fig Sterilization and pasteurization of food products
Technology of typical food /food prod ucts (bread, cheese, idli.)
-
BIO:EECHNO LOG Y
I ::\~~lat:i'l m l,f inrl ustri all y important m LiCr[)OI'f\.MLislY1~ for microbial pwces_ ,,~
2 Dotrrnl I!VltlOfl of [henna! dea th po ln+ {TDPI and thermal death OnH~ (TDT) uf microorganism for dpsign t)1 ,1 ';;h . -ri I izer
De l.errn l rta ti urt of SHWV th ,; u rove of a s uppH ed m icr.oorga rris m and a lS.'l~ to determine substrate
degmd.J tiorr profile
(b) Computer Specific growtb r.1h~ Hd, growth yi sld (Y,. L RfW(1tLl.- product formation ['<:'I t~ (Q) ~md ~.p ccific substrate corrsu motion rutc {Q ).
. I' ~.~
+, Comparative slt .. idles ot ethanol production usin~ d!tfer~f1t substrates
3, \-1 irrobial I~ reduction uf citric: acid u ... sing Aspergillus niger:
f1, vlicrobial production of <1nl i h luth.:t; (penicillin)
r. Pruducrion and estimation of alkaline protease
K Sa LH:'t Krant fL'rml:nta.tion
9_ USC' of aIghl.Mc for cell immobll i %.-a till n
Ill, Study of rhe{:1lngy Llf viscous polymers and microbial cultures
I I Dctcrm i na lion fit uxygen transfer rate and Kla
12. Deh·m~tnat\oil of mixing time in. binrrj"~<tof<';'
Books
Biechemlcal lngi[1t;t'ring, Aiba S.,. Humphrey .. A.L and Millis. 1 .F .. Universitv, of '·1 okvo Press, Tokyo.
3 Biouhemical Engint'l'ung Fundamentals; Baily_ J-E.. and Ollis. D.F.,. _ kGr:::1;w-Hill B~)lIk
en_, ,"c'w y~jfk.
~ - Btuprocl'SS 1 echnology: FW"IdarnentaIs. and Applica Lions; KTfC Stockholm,
:;- Process l:.J1gin.t;'f?['!ng in Biotechnel 'gy, jackson, AT., frel tice I Iall, Engel"" .. od Ctiffs.
6 Bioprocess Enginecrlng: i3a"i(" Concepts, Shuler. M,L., and f(;:'Irgl.F:, [)nmlice Hall, ErtghnvoDd Cliff,,_
7, Prinl~i.pJl-"~ uf Fermentation Techno] . .[:'Igy r Stanbury. P.F.> arid \'Vhit<lk~L A., ['ergi'lnlon 1"'1'1;-,%, OXford.
8 Bicreacrion Engineeri ng Pri nciples, Nielson, J~ .a.nd V i lladsen, J., Plenum Pless.
Y, Chernlcal LnglT'l~t'rlng Problems in B:iotechl1oiog';'" Schuler. M. L (ed.), A[CHE:
10_ tltO(hcmlC;),l Engine "ring, Lee. j.M .. , Prentice Hall Inc.
•
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U. Bioprocess Engin er ing-Kinetics. Mags Transport, Reactorsand Gene Expressinn; Vieth. W.F.,John Wiluy & Sons Inc.
18 Genomlcs and tunctional glsnomics
Wh ore gl:'MJme <1.11 illy:;;:] 5: Prepara ti )11, of ordered cosrn id li braries, ba de-ria 1 artificia J chromosome libraries, shotgun Ii braries and s~>queJ1ci.ng, Conventional sequencing (Sanger, Maxam and Gilb!cTt methods), automated sequencing,
DNA Microarray: Pl'in'l-illg of oligonudeotisie!;; and peR product. on glass slides, nitrocellulose paper. Gen'orne analysis for glub.}r patterns of gene expression. using fluorescent-labelled eDNA or end-labt'JI,t-d Rr\IA probes, Analysis 1f sin.gJenudeotidf.' polymorphism using DNA chips.
Proteome analys is: Two dirncnr ionalscparation (if total cellular protein», isolation and. s.~qucnc(' ,21n~llr~is of individual protein spots by mass fipedroscopy. Protein microarrays, Advan l?j~('s and d isadvantages of DN A and protein microarrays.
Reference:
1 Recent articles in iournals
2. The Internet and .:\1£'\ ...... Bjo~ogy : Tools. fOI~ GellOJrlic and Molecular Research by Peruski, Jr. and I'eruski (Asrvl) 1997 .
. ), Functional G,momicl1: A I'racti,c·,d Approach, Stephen P. Hunt ,and Rick Livesey, eds., 'Oxford Unlversity Pl'e~5T 2000,
.t. Df\l A Microarrays: a Practical Approach, Mark Srhlena, (::'<1., Oxford University Fre.%.
19 Elective Paper
20 Project