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SalemBelay

Mrs.Peterson
APBiology
26February2016
ComparingDNASequencesToUnderstandEvolutionaryRelationships(BLAST)
PreLabQuestions:
1.
FloweringPlants
PineTrees

Mosses
Ferns

Flowers
Seeds
VascularTissue

2.Theycouldhavethesameaminoacidsequencesbutcodefordifferentcodons.
Humans
Roundworm

Chimps
FruitFly

Dog

Bipedal
Thumbs
Mammal
Legs

Hypothesis:
Ifthefossilspecimenweretobeplacedonthecladogram,thenitwouldbelocatedata
newbranchthatdeviatesfromthecrocodilians.

Observations:
GeneralObservations:
Initialreactionisthatitlookslikesomesortoflizard
Verylongtail
Lookslikeasmallcreature
Couldalsobeadinosaurofsomesort
Veryskinnylegsonlyseemtoseethreebutonecouldvebeendamaged
Initialsketchattached:

Gene
Sequenc
e
1

Gallus,gallus

Collagen

10129

0.0

Commo
nname
of
species
Chicken

Drosophila

Melanogaster

4419

0.0

Fruitfly

Taeniopygia
Guttata
AlligatorSinesis

Ubiquitin

2224

0.0

Mitochondrio
n

1768

0.0

Zebra
finch
Chinese
alligator

Specificnameof
ClosetMatching
Species

GeneName

Max
Score

E
Value

ObservationsFrom
DistanceTreeof
Results
Verycloselyrelatedto
birds
Verycloselyrelatedto
flies
Verycloselyrelatedto
birds
Verycloselyrelatedto
vertebrates

Datatable:
Analysis:
1. BasedontheBLASTresultsfromeachgene,whichspeciesaremostclosely
relatedtotheunknownfossilspecimen?Comparethescoresforeachgene.
Theonewiththehighermaxscore(andlowestEvalue),thecloserthe
alignment?
ThespeciesthatismostlikelyrelatedtotheunknownfossilisGallusCollagen,asithas
thehighestmaxscorecomparedtotheotherspecies(scoreof10129).Thesecondclosest
isDrasophilaMelanogasterwithamaxscoreof4419.NextisTaeniopygiaUbiquitin
(2224),andAlligaorSinesisMitochondrionlast(1768).TheEValueisirrelevantinthis
scenariobecausetheyallhavethesameEValueof0.0.
2. Basedonthisinformation,whereshouldthenewfossilspeciesbelongonthe
cladogram?Ifnecessary,redrawthecladogramyoucreatedbefore.

Thenewfossilspecieswouldbebranchedoffofthebirds.Thisisbecauseitisclosest
relatedtoGallusCollagen,whichismostsimilartobirds.

Conclusion:

1. WasyourhypothesissupportedbytheDNAdata?
No,theinitialhypothesiswasnotsupportedbytheDNAdata.Thehypothesiswasthatit
wouldbeclosestrelatedtocrocodilians,butitwasactuallyclosestrelatedtobirds.In
fact,thespeciesmostrelatedtocrocodilians(AlligatorSinesis)hadthelowermaxscore
(1768)andwasthereforetheleastcloselyrelated.
2. Compareanddiscussyourcladogramwithyourclassmates.Doeseveryone
agreewiththeplacementofthefossilspecimen?Ifnot,whatisthebasisof
thedisagreement?
Yes,mostoftheclassagreesthatthisspeciesismostcloselyrelatedtoGallusCollagen
andispartofthebirdsbranch.
3. Whatotherdatacouldbecollectedfromthefossilspecimentohelpproperly
identifyitsevolutionaryhistory?
Otherphysicalfeaturescouldhelpidentifythefossilspecimen.Thesecouldbe:
1. Ifthespecieshadears
2. Ifthespecieshadscales
3. Ifthespecieshadteeth
Dataaboutitsenvironmentalconditionsandtimeperiodcouldalsobehelpfultoidentify
itsevolutionaryhistory.

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