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Mrs.Peterson
APBiology
26February2016
ComparingDNASequencesToUnderstandEvolutionaryRelationships(BLAST)
PreLabQuestions:
1.
FloweringPlants
PineTrees
Mosses
Ferns
Flowers
Seeds
VascularTissue
2.Theycouldhavethesameaminoacidsequencesbutcodefordifferentcodons.
Humans
Roundworm
Chimps
FruitFly
Dog
Bipedal
Thumbs
Mammal
Legs
Hypothesis:
Ifthefossilspecimenweretobeplacedonthecladogram,thenitwouldbelocatedata
newbranchthatdeviatesfromthecrocodilians.
Observations:
GeneralObservations:
Initialreactionisthatitlookslikesomesortoflizard
Verylongtail
Lookslikeasmallcreature
Couldalsobeadinosaurofsomesort
Veryskinnylegsonlyseemtoseethreebutonecouldvebeendamaged
Initialsketchattached:
Gene
Sequenc
e
1
Gallus,gallus
Collagen
10129
0.0
Commo
nname
of
species
Chicken
Drosophila
Melanogaster
4419
0.0
Fruitfly
Taeniopygia
Guttata
AlligatorSinesis
Ubiquitin
2224
0.0
Mitochondrio
n
1768
0.0
Zebra
finch
Chinese
alligator
Specificnameof
ClosetMatching
Species
GeneName
Max
Score
E
Value
ObservationsFrom
DistanceTreeof
Results
Verycloselyrelatedto
birds
Verycloselyrelatedto
flies
Verycloselyrelatedto
birds
Verycloselyrelatedto
vertebrates
Datatable:
Analysis:
1. BasedontheBLASTresultsfromeachgene,whichspeciesaremostclosely
relatedtotheunknownfossilspecimen?Comparethescoresforeachgene.
Theonewiththehighermaxscore(andlowestEvalue),thecloserthe
alignment?
ThespeciesthatismostlikelyrelatedtotheunknownfossilisGallusCollagen,asithas
thehighestmaxscorecomparedtotheotherspecies(scoreof10129).Thesecondclosest
isDrasophilaMelanogasterwithamaxscoreof4419.NextisTaeniopygiaUbiquitin
(2224),andAlligaorSinesisMitochondrionlast(1768).TheEValueisirrelevantinthis
scenariobecausetheyallhavethesameEValueof0.0.
2. Basedonthisinformation,whereshouldthenewfossilspeciesbelongonthe
cladogram?Ifnecessary,redrawthecladogramyoucreatedbefore.
Thenewfossilspecieswouldbebranchedoffofthebirds.Thisisbecauseitisclosest
relatedtoGallusCollagen,whichismostsimilartobirds.
Conclusion:
1. WasyourhypothesissupportedbytheDNAdata?
No,theinitialhypothesiswasnotsupportedbytheDNAdata.Thehypothesiswasthatit
wouldbeclosestrelatedtocrocodilians,butitwasactuallyclosestrelatedtobirds.In
fact,thespeciesmostrelatedtocrocodilians(AlligatorSinesis)hadthelowermaxscore
(1768)andwasthereforetheleastcloselyrelated.
2. Compareanddiscussyourcladogramwithyourclassmates.Doeseveryone
agreewiththeplacementofthefossilspecimen?Ifnot,whatisthebasisof
thedisagreement?
Yes,mostoftheclassagreesthatthisspeciesismostcloselyrelatedtoGallusCollagen
andispartofthebirdsbranch.
3. Whatotherdatacouldbecollectedfromthefossilspecimentohelpproperly
identifyitsevolutionaryhistory?
Otherphysicalfeaturescouldhelpidentifythefossilspecimen.Thesecouldbe:
1. Ifthespecieshadears
2. Ifthespecieshadscales
3. Ifthespecieshadteeth
Dataaboutitsenvironmentalconditionsandtimeperiodcouldalsobehelpfultoidentify
itsevolutionaryhistory.