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S0 (mmol/l) V (mmol/min)

9.524
20
30.303
40
62.5
100
Km
Km
Km
Km
Km
Km
promedio

0.971
1.389
1.667
2
2.174
2.5

1/S

1/v

0.1049979
0.05
0.033000033
0.025
0.016
0.01

1.0298661
0.7199424
0.59988
0.5
0.4599816
0.4

1/Vmax
Vmax

0.3559
2.809778

vmax(promedio)

1/Vmax
Vmax

2.9214192

Lineweaver-Burk
1.2
1

f(x) = 6.5880363107x + 0.3558571809


R = 0.9882961078

0.8

1/v 0.6
0.4
0.2
0

0.02

0.04

0.06

1/[S]

0.08

0.1

0.12

v/s
0.101953
0.06945
0.0550111
0.05
0.034784
0.025
0
0.3297
3.0330604

Km

v/S
9.524
20
30.303
40
62.5
100

v
0.971
1.389
1.667
2
2.174
2.5
2.921419
20.235

v
9.8084449
14.398848
18.178164
20
28.74885
40

KM
19.130577
22.065068
22.803035
18.42838
21.487437
16.85676
20.128543

Hanes-Woolf
Eadie-Hofstee

45
40

3.5
f(x) = 0.3297297786x
+ 7.439547297
R = 0.9960584159
3

35
30

2.5

25

[S]/v 20

15

1.5

10

0.5

f(x) = - 20.0526310483x + 2.9091533962


R = 0.9752334466

20

0 40

60
0.02

[S]

80
0.04

1000.06

v/[S]

120

0.08

0.1

Hofstee

962

0.06

/[S]

120

0.08

0.1

0.12

S (mg/L)

Sin inhibidor

Inhibidor 1

Inhibidor 2

1
2
5
10
20
1
2
5
10
20
1
2
5
10
20

Vo

0.45
0.4
0.35
0.3
0.25

1/V

0.2
0.15
0.1
0.05
0

5
20
f(x) = 0.3016731419x
+ 0.098664981
8
12.6
f(x) = 0.2854434311x + 0.0515576477
15
15.2
18
10
3
f(x) = 0.1508365709x + 0.0493324905
5
5
8.8
0
12
0
5
18
2.5
0.2
0.4
0.6
0.8
1
4
1/[S]
20
6.3
7.6
15
9

Sin inh

10
1.2

Con Inhi

10

1
0.2 0.33333333
0.4
0.5
0.125
0.2
0.25
0.2 0.07936508 0.11363636 0.15873016
0.1 0.06578947 0.08333333 0.13157895
0.05 0.05555556 0.05555556 0.11111111

5
0

10

Sin inhibidor

Con Inhibidor 1
20

98664981

515576477

15
Sin Inhibidor
Linear (Sin Inhibidor)

493324905

Linear (Inhibidor 1)

Inhibidor 2

10

Inhibidor 1

5
0.8

10
1

15

Linear (Inhibidor 2)
20

25

10

1.2

Con Inhibidor 2

1 Vm
Km
2 Vm
Km

10

15

20

25

15

Con Inhibidor 1

10

15

5.53403431
10.1317123
3.05903946
19.379845

20

25