The Extraction

Procedure

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To perform an extraction, for example, using a Soxhlet extraction apparatus, the
following equipment, accessories and expendables are needed:
Accessories and Expendables
Stock Number:
Evaporating dish, 70 mm diam., glass

QL9000113

Beaker, 100 ml

VG1121116241

Desiccator, DN 150 (for a single extraction)

QL9042031

with desiccator plate

QL9042514

Desiccator, DN 300 (for multiple extractions)

QL9042047

with desiccator plate

QL9042528

Homogenizer with stand, clamps and accessories

QL9571195

Analytical balance with 0,001 accuracy, for example QL6250429

Plastic spatula / spoon combination

80596

Drying oven
Extraction thimbles for the 100 ml Soxhlet

VG804841034

Boiling chips

VG804830700

Silica gel, 1 kg

CBKIESELGEL1L

Cotton, 400 g

QL9413151

Extraction solvent, for example

Petroleum ether; boiling fraction 40 60 C
Sodium sulfate, anhydrous
Sand

Equipment
Stock No.

Model

No. of
samples

Control of
heating power

Time-Temp.
Control

804800522

KEX 100 F

804880023

In-line heating
unit R 104 S

Individually for
each sample

804880022

In-line heating
unit R 106 S

Individually for
each sample

Heating block
system
804850020
804850025

DET
(DBAS +
DFE 100)

Preparation for the

Extraction

Place 3 or 4 boiling chips into the

solvent vessel (e.g round bottom
flask or cylindrical flask).

Dry the solvent vessel in a drying

oven to constant weight
(about 1 hour) at 1032 C

Fill the silica gel into the desiccator

and insert the desiccator plate..
Allow the desiccator to cool to
room temperature
(about 30 minutes).

Weigh the solvent vessel

containing the boiling chips to
an accuracy of 1 mg.

Sample Preparation

Homogenize or grind the

sample.

Ground
sample

dry

0.000

ON
OFF

TARE

weigh

moist

0.000

ON
OFF

liquid

TARE

weigh

weigh

dry

mix

wipe

cover

extract

drain

Dry Samples

Weigh the ground dry sample into the

extraction thimble.
For example, weigh out 6.6 g with an
expected fat content of at most 2 g.

Moist Samples

Moist samples must be dried for

2 3 hours at 1032 C in a drying
oven after having been weighed in
the extraction thimbles.

Liquid Samples Containing

Water Content

Weigh the liquid sample into an

evaporating dish

Mix the liquid sample with sand

and anhydrous sodium sulfate.

Use a cotton swab, which has

been moistened with extraction
solvent, to wipe the sample into
the extraction thimble.

Extraction
Close the extraction thimble with a
fat-free cotton wad
Insert the thimble into the Soxhlet
extractor
If the Soxhlet extractor has a spigot for
solvent draining, close the spigot.

Fill the solvent into the solvent vessel.

Extract at a temperature of 110-130 C
for 20 30 extraction cycles
(4 6 hours), depending on the nature
of the sample and the solvent
employed.

Drain the solvent into a suitable container

by opening the spigot on the Soxhlet
extractor.
Continue to heat the solvent vessel until
all of the solvent has been evaporated
and condensed in the Soxhlet extractor
(spigot closed). Care must be taken not to
heat the fat residue to decomposition.
The thus recovered solvent may be
reused for subsequent extractions.

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Place the vessel containing
the fat residue in a drying
oven at 1032 C and heat to
constant weight (indicating
evaporation of all solvent.
After every completion of the
solvent evaporation step, air
the drying oven out by
leaving the door open.
Observe carefully all safety
guidance of the oven
manufacturer.

Allow the vessels

containing the fat to
cool to room
temperature (about 30
minutes).

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Weigh the vessel

containing the boiling chip
and fat residue.
Compute the fat content according to the
following formula:
F [%] =

m 2 m1
100 where
E

m1

is the weight of the dry empty vessel, in

grams, including the boiling chips,

m2

is the weight of the vessel, in grams,

containing the boiling chips and fat residue
after evaporation of the solvent and

is the sample weight in grams.

behr Labor-Technik GmbH Spangerstrae 8 D 40599 Dsseldorf/Germany

Tel. +49 (0)211 748 47 17 Fax: +49 (0)211 748 47 48 email: j.behr@behr-labor.com http://www.behr-labor.com