Escobedo LN, Lpez C, Mndez MV.

Expression of integrin B1 and vinculin as cellular adhesion marker in

materials for retrograde obturation
Aim: To assess the expression of integrin B1 and vinculin as cellular adhesion
marker of 3 materials for retrograde obturation at 1 hour, 5 days and 10 days.
Introduction: Periapical surgery, which makes up 3-10% of all procedures in
endodontic practice1, has a success rate of 85-94% corresponding to
apicoectomy2,3. After the root resection and retrograde cavity preparation, a
material of retroobturation is placed. The objective is to provide an apical seal
that prevents the migration of antigens from the root canal to the periradicular
tissues or viceversa4.
These materials will be in close contact with the surrounding tissues. Thus, the
biocompatibility of the material is of crucial importance to avoid adverse
reactions in the periapical tissues5.
Materials and methods: Cells were obtained from an oral mucus biopsy of a
healthy patient with the previous informed consent. Materials were prepared
according to the manufacturers instructions under aseptic conditions. The
specimens were kept in an incubator for the length of time set by the
manufacturer (15 minutes for the IRM and Super EBA and 4 hours for the MTA).
The specimens were sterilized under ultraviolet light. 1 ml of cell growth
medium with fibroblast (5x107) was added to them and they were placed in an
incubator at 37 C for 1 hour, 5 days and 10 days. A control group was used
during the process. After the period of observation was finished, the samples
were evaluated with the live/dead kit and phalloidin. The expression of
adhesion proteins integrin-1 and vinculin was also evaluated. The cellular
adhesions were studied due to the presence or absence of filopodia and
lamellipodia.
Conclusion: The statistic analysis was performed through the HSD Tukey and
ANOVA tests. The MTA group showed the highest levels of protein expression
and focal adhesion, followed by IRM and Super EBA. Also, MTA had a higher
expression of integrin-1 and vinculin, followed by IRM and EBA. These last two
showed no statistic difference of significance. IRM showed the lowest focal
adhesion levels, whilst the MTA group had the highest rate. This result suggests
good biocompatibility for MTA as it showed the highest level of adhesion.

20

40

10

20

60

30

80

40

50

60

E xpresin de B 1 integrina en unidades arbitrarias

A d h e sio n e s F o ca le s e n u n id a d e s a rb itra ria s

Focal adhesions in arbitrary units/ Days (CAMBIAR POR FAVOR)

Control
MTA
SuperEBA
IRM

1
5

1
10

Das

5
10

Das

EXPLICACIN DE LA TABLA ANTERIOR: Cell viability expression in control and

experimental groups at 1 hour, 5 days and 10 days.

Images of fibroblasts taken with a confocal

microscope (Leica DMI400B). Image 6. Control
group 10 days phalloidin and Sytox. Image 7.
MTA, 10 days without phalloidin and Sytox.
Image 8. Super EBA, 10 days without phalloidin
and Sytox. Image 9. IRM, 10 days without
phalloidin and Sytox.

1) Johnson BR et al. Periradicular

Surgery. In: Cohen S, Hargreaves
KM, editors. Pathways of the Pulp.
9th ed. Missouri: Mosby; 2006. pp.
72485.
2) Sumi Y et al. Ultrasonic root-end preparation: clinical and
radiographic evaluation of results. J Oral Maxillofac Surg 1996; 54:
590-3.

3) Testori T et al. Success and failure in periradicular surgery. A

retrospective analysis. Oral Surg Oral Med Oral Pathol 1999; 87:
493-8.
4) Torabinejad M et al. Antibacterial effects of some root end filling
materials. J Endod, Vol 21, No 8, 1995.
5) Buddy D. Ratner Biomaterials Science 2 Edition, Ed. Academic
Press Inc. 1996.
6) Friedman S. The Prognosis and expected outcome of apical
surgery. Endodontic Topics 2005, 11, 219-262.(Courtesy of Dr
Richard Rubinstein, Farmington Hills, MI, USA.)

EN LA IMAGEN DE LA APICECTOMA PONER REFERENCIA 6