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Reactions (4 min) were 1.250 mL in total, with 2 mM 4-NPP in 0.5 M Tris, pH 9.2, excepting variations as noted
Each datum is the mean of 3 independent measurements of A400 for the time indicated.
time
Standard
Reaction
No E
phosphate
pH 7.2
Mg2+
supplement
EDTA
Mg2+ rescue
0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.087
0.001
0.010
0.014
0.131
0.007
0.089
0.174
0.002
0.018
0.029
0.263
0.014
0.179
0.262
0.002
0.029
0.042
0.396
0.021
0.267
0.346
0.002
0.038
0.056
0.526
0.027
0.356