Curriculum Vita of S. Brookhart Shields, Ph.D.

Visiting Assistant Professor

Department of Biology | DIRECT 507-933-7330|CELL 319-331-2484| bshields@gustavus.edu | brookhartshields.weebly.com
800 West College Avenue | St. Peter, Minnesota 56082| gustavus.edu

Education

Postdoctoral Fellow

Cell Biology and Biophysics

2007-2011

Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA

Advisor: Robert Piper, Ph.D.
Research Area: Characterization of Ubiquitin-Sorting Receptors of the Multivesicular Body
Degradation Pathway in Yeast. Down-regulation of plasma membrane receptors involves their
ubiquitination, transport to endosomes and eventual sorting into lytic compartments. This
research focused on identifying and characterizing the ubiquitin-receptors that operate in the
endocytic pathway.

Doctorate of Philosophy

Bioinoganic Chemistry

2005

Department of Chemistry, University of Iowa, Iowa City, IA

Adviser: Sonya Franklin, Ph.D. (Currently employed at Monsanto)
Thesis: Design, Characterization, and Reactivity of Metallopeptides Based on the Metal Binding
Regions of Avian and Mammalian Prion Proteins. Prion proteins are thought to have a function in
protecting neuron from excitotoxicity by binding to copper ions released during synaptic
transmission. However, the role that copper plays in the disease states prion protein, such as
Mad Cow disease, was unknown. This research focused on characterizing the metal binding
properties of model peptides of prion proteins.

Bachelors of Science

Chemistry

Winona State University, Winona, MN

Advisor: David Rislove, Ph.D.
Minor: Biology, Emphasis in Ecology , Concentration Area: Communication Studies

1998

Teaching Experience ( Denotes de nova course)

Visiting Assistant Professor

2015-present

Department of Biology, Gustavus Adolphus College, St. Peter, MN

(7-course load/year)
Course
Level
Size
Year
Principle of Biology, Labs
100-level majors
15
2015
Cell and Molecular Biology, Lecture & Labs
200-level majors
14
2015
Biology Explorations, Lecture & Labs
100-level, non-majors
24
2016
Cell Biology, Lecture and Lab
300-level
16
2016
Social and Creative Knitting, Lecture and Lab
100-level, Jterm
2016

Visiting Assistant Professor

Department of Biology, Luther College, Decorah, IA

Course
Creative Problem Solving in Science
Principles of Biology I:
Ecology, Evolution, and Biodiversity Labs
Principles of Biology II:
Molecules, Cells, and Genes Lecture & Labs
Cell Biology, Lecture and Lab
Introduction to Biophysics of the Cell
Seminar in Science Communication
Senior Thesis Projects

2011-2015

(7-course load/year)
Level
Size
Year
100-level non-majors
20-24
2012, 2014
Team-taught,
16-18
2011- 2014
100-level
Team-taught,
220 lect, 2012- 2015
100-level
20 lab
300-level
14-16
2011, 2013
200-level
16-18
2012-2015
300-level
8
2014
300-level
5-6
2012-2015

Teaching Experience contd

Lecturer

2009-2010

Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA

Course
Human Physiology for Dental Students
Human Physiology for P.A. Students

Level
Post-graduate
Post-graduate

Involvement
5 lectures
5 lectures

Size
>200
35

Guest Lecturer

Year
2009, 2010
2010, 2011

2008

Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA

Course
Advanced Cell Biology

Level
Post-graduate

Involvement
1 lecture

Size
20

Graduate Teaching Assistant

Year
2008

1999-2003

Department of Chemistry, University of Iowa, Iowa City, IA

Course
Inorganic Chemistry Lab
Principles of Chemistry Lab
Organic Chemistry I
Tech & Society Lab

Level
300-level
100-level, majors
200-level
100-level, nonmajors

Involvement

Size

Principle TA
Lab instructor
Discussion Leader
Lab instructor

18
24
20
24

Year
1999- 2001, 2003
2000, 2002-2003
2000
2002

Publications (*Denotes undergraduate co-author.)

BMC Biotechnology

2012

A set of dual promoter vectors for high throughput cloning, screening, and protein expression in
eukaryotic and prokaryotic systems from a single plasmid.
Namita Sinah *, Charollet Williams*, Robert C. Piper, S. Brookhart Shields.
DOI: 10.1186/1472-6750-12-54

Traffic

2011

Journal of Cell Biology

2009

How Ubiquitin Functions with ESCRTs

S. Brookhart Shields and Robert C. Piper
DOI: 10.1111/j.1600-0854.2011.01242.x

"ESCRT Ubiquitin Binding Domains Function Cooperatively During MVB Cargo Sorting"
S. Brookhart Shields, Andria J. Oestreich, Stanely Winistorfer, Doris Nguyen, Johanna A. Payne,
David J. Katzmann, and Robert C. Piper
DOI: 10.1083/jcb.200811130
This article was the subject of a mini-review in the same issue and featured in Faculty of 1000
Biology.

Journal of Inorganic Biochemistry

2007

Biochemistry

2004

Investigation of the affinity and selectivity of avian prion hexarepeat peptides for physiological
divalent metal ions.
S. Brookhart Shields and Sonya J. Franklin
DOI:10.1016/j.jinorgbio.2007.01.004
De novo Design of Cu (II) Helix-Turn-Helix Chimeras: The Prion Octarepeat Motif in a New
Context
S. Brookhart Shields and Sonya J. Franklin
DOI: 10.1021/bi048555k

C.V. of S.B. Shields

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Presentations * Denotes undergraduate coauthors.

Midstates Consortium for Math and Science

2013

Undergraduate Research Symposium for Biological Sciences and Psychology

Title: Investigating the Selective Degradation of Ald6p by Autophagy in Saccharmyces
cerevisiae
Authors: Julia Mandsager*, Sarah Floden*, Kelsi Swanson*, and S. Brookhart Shields

American Society of Cell Biology 48th Annual Meeting

2008

Metalloprotein and Protein Design Conference

2005

Title: "ESCRT Ubiquitin Binding Domains Function Cooperatively During MVB Cargo Sorting"
Authors: S. Brookhart Shields, Andria J. Oestreich, Stanely Winistorfer, David J. Katzmann, and
Robert C. Piper
Title: Investigation of Prion Repeat Domains: Metal Binding, Coordination, and Selectivity
Authors: S. Brookhart Shields and Sonya J. Franklin

36th Annual Great Lakes Regional American Chemical Society Meeting

2004

Title: Characterization of Chimeric Copper-Binding Peptides Based on Prion Proteins

Authors: S. Brookhart Shields and Sonya J. Franklin

36th Annual Central Regional American Chemical Society Meeting

2004

Gordon Graduate Research Conference

2003

Title: Characterization of Chimeric Copper-Binding Peptides Based on Prion Proteins

Authors: S. Brookhart Shields and Sonya J. Franklin
Title: Characterization Bioinorganic Chemistry De novo Design of Cu (II) Helix-Turn-Helix
Chimeras: The Prion Octarepeat Motif in a New Context
Authors: S. Brookhart Shields and Sonya J. Franklin

Center for Biocatalysts and Bioprocessing 12th Conference

2003

38th Midwest Regional American Chemical Society Meeting Conference

2003

Center for Biocatalysts and Bioprocessing 12th Conference

2002

Title: De novo Design of Cu (II) Helix-Turn-Helix Chimeras: The Prion Octarepeat Motif in a New
Context
Authors: S. Brookhart Shields and Sonya J. Franklin
Title: De novo Design of Cu (II) Helix-Turn-Helix Chimeras: The Prion Octarepeat Motif in a New
Context.
Authors: S. Brookhart Shields and Sonya J. Franklin
Title: De novo Designed Metalloproteins Based on ATX1 Copper Chaperones and Homeodomain
Proteins
Authors: S. Brookhart Shields and Sonya J. Franklin

224th National American Chemical Society Meeting

De novo Designed Helix-Turn-Helix Metalloproteins
Authors: S. Brookhart Shields and Sonya J. Franklin

C.V. of S.B. Shields

2002

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Service
Faculty Member

2011-2015

Luther College
Heath Sciences Club Faculty Adviser
Pre-Health Professions Adviser
Student Academic Adviser (10-25 students/semester)
Member of the National Association of Advisors to the Health Professions
Participant in Diversity Center Program Review

Organizing Chair

2008-2010

Postdoctoral Enhancement Group Committee

Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA

Postdoctoral Committee Member

2009-2011

Women in Cell Biology Committee

American Society of Cell Biologists

Safety Committee Member

2004-2005

Department of Chemistry, University of Iowa

Scholarship Selection Committee Member

2005

Iota Sigma Pi, Honor Society for Women Chemist

Other Research Experience

Scientific Support Staff

2006-2007

Carver College of Medicine NMR Facility, University of Iowa, Iowa City, IA

Supervisor: S. Ramaswamy, Ph.D. (now Senior Professor and Dean of inStem, the Institute for
Stem Cell Science and Regenerative Medicine in Bangalore, India)
Primary responsibility was to provide administrative and scientific support for newly constructed
NMR facility including user training, maintenance, and analysis service.

Graduate Research Assistant

2004-2005

High Resolution Mass Spectrometry Facility, University of Iowa, Iowa City, IA

Supervisor: Lynn Teesch, Ph.D.
Primary responsibility was providing mass spectrometry analysis services to the University
community. Experience included operation of ESI-MS and LC-ESI-MS instruments, sample
handling and preparation, and data acquisition and interpretation.

Extramural Funding
Research Grant

2014

Monticello College Foundation

$6850
Competitive grant paying for student stipend, housing, travel, and research supplies for one
female student researcher for an 8-week research experience, as well as an additional research
assistant student.

Research Grant

2013

Better Futures for Iowans

$500
Competitive research grant covering expenses of using the core facilities at the Carver College of
Medicine, the University of Iowa.
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Extramural Funding-contd
Post-Doctoral Fellowship

2009- 2010

National Institutes of Health Institutional Training Grant: Training in Kidney Disease, Hypertension,
and Cell Biology
A competitive institutional grant providing postdoctoral fellowships. An institutional review
committee, headed by Dr. John Stokes, Internal Medicine, selects fellows. Wrote grant proposal,
complied application materials, and designed and preformed experiments for preliminary data.
Fellowship funded two years of salary support.

Post-Doctoral Fellowship

2007- 2009

American Heart Association Heartland Affiliate Postdoctoral Fellowship

Wrote grant proposal, complied application materials, designed experiments, and performed
experiments for preliminary data. Fellowship funded two years of salary and indirect costs.

Intramural Funding
Research Grant

2014

Luther College Deans Office Student/Faculty Collaborative Research Grant

$4000
Competitive research grant paying student stipend, housing, and research supplies for one
student researcher for an 8-week research experience.

Research Funds

2014

Luther College Benson-Brecke Student Research Fund

$1000
Competitive research funds to support student research.

Research Grant

2013

Luther College Deans Office Student/Faculty Collaborative Research Grant

$4000
Competitive research grant paying student stipend, housing, and research supplies for one
student researcher for an 8-week research experience.

Research Funds

2013

Benson-Brecke Student Research Fund

$650
Competitive research funds to support student research.

Research Grant

2013

John Tjostem Undergraduate Research Fund Endowment

$4600
Competitive research grant paying student stipend, housing, and research supplies for one
student researcher for an 8-week research experience.

Supplemental Travel Funds

2013

Faculty Development Funds

$1000
Travel funds that allowed travel to University of Iowa for a 4-week research experience.

C.V. of S.B. Shields

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Honors
WICB Childcare Grant

2009

Women in Cell Biology Committee, American Society of Cell Biology

University of Iowa Graduate Student Senate Award

2004

University of Iowa

University of Iowa Student Government Scholarly Presentation Grant

2004

University of Iowa

Dr. Eunice Schuytema Beam Women in Science and Engineering Travel Grant

2004

University of Iowa

American Chemical Society Undergraduate Award in Analytical Chemistry

1997

Winona State University

Minnesota Valley Testing Laboratories Summer Internship

1997

Minnesota Valley Testing Laboratories

Additional Education
Developmental Biology Teaching Workshop

2013

Darling Marine Center, University of Maine, Walpole, ME

Science Pedagogy and Practice

2013

Augustana College, Rock Island, IL

New Advisors Workshop

2013

Central Association of Advisors for the Health Professions Regional Meeting

Detroit, MI

Advancing Biomedical Research Workforce Diversity

2010

NIGMS Workshop for Postdocs Transitioning to Independent Positions

National Institutes of Health, Bethesda, MA

User Training Course: Pulse Programming for Multidimensional NMR

2007

Bruker Bio-Spin Billerica, MA

User Training Course: Avance II: Advanced Training for Multidimensional NMR

2007

Bruker Bio-Spin Billerica, MA

User Training Course: Bio-NMR: NMR for Biological Molecules

2007

Bruker Bio-Spin Billerica, MA

NMR Data Collection and Analysis for Biological Structure Determination

2006

National Magnetic Resonance Facility at Madison, Madison, WI

CEGS/NMRFAM 2006 Wheat Germ Cell-Free Protein Production Workshop

2006

Center for Eukaryotic Structural Genomics

University of Wisconsin, Madison, WI

C.V. of S.B. Shields

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