Materials & Streams Report

February 13, 2015

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1. OVERALL PROCESS DATA
Annual Operating Time
Recipe Batch Time
Recipe Cycle Time
Number of Batches per Year
MP = Undefined

7,687.27h
613.27h
294.75h
25.00

- Page 1 -

2.1 STARTING MATERIAL REQUIREMENTS (per Section)

Section

Starting Material Active Product

Amount
Needed
(kg
Sin/kg
MP)

Main Section

(none)

Unknown Unknown Unknown Unknown

(none)

Molar
Yield
(%)

Mass
Yield
(%)

Gross
Mass
Yield
(%)

Sin = Section Starting Material, Aout = Section Active Product

2.2 BULK MATERIALS (Entire Process)

Material

kg/yr

kg/batch

Glucosa
Water
Biomass
Air
TOTAL

15,750
522,220
0
5,289,215
5,827,185

630.000
20,888.791
0.010
211,568.614
233,087.415

kg/yr

kg/batch

15,750
522,220
0
5,289,215
5,827,185

630.000
20,888.791
0.010
211,568.614
233,087.415

% Total

kg/yr

kg/batch

100.00
100.00

15,750
15,750

630.000
630.000

kg/kg MP

2.3 BULK MATERIALS (per Section)

SECTIONS IN: Main Branch
Main Section
Material
Glucosa
Water
Biomass
Air
TOTAL

kg/kg MP

2.4 BULK MATERIALS (per Material)

Glucosa
Procedure
Main Section (Main Branch)
P-1
TOTAL

kg/kg MP

- Page 2 -

Water
Procedure
Main Section (Main Branch)
P-1
TOTAL

Biomass
Procedure
Main Section (Main Branch)
P-2
TOTAL

Air
Procedure
Main Section (Main Branch)
P-2
P-7
TOTAL

% Total

kg/yr

kg/batch

100.00
100.00

522,220
522,220

20,888.791
20,888.791

% Total

kg/yr

kg/batch

100.00
100.00

0
0

0.010
0.010

% Total

kg/yr

kg/batch

11.11
88.89
100.00

587,668
4,701,547
5,289,215

23,506.723
188,061.892
211,568.614

kg/kg MP

kg/kg MP

kg/kg MP

2.5 BULK MATERIALS: SECTION TOTALS (kg/batch)

Raw Material
Glucosa
Water
Biomass
Air
TOTAL

Main Section
630.000
20,888.791
0.010
211,568.614
233,087.415

2.6 BULK MATERIALS: SECTION TOTALS (kg/yr)

Raw Material
Glucosa
Water
Biomass
Air
TOTAL

Main Section
15,750
522,220
0
5,289,215
5,827,185

- Page 3 -

3. STREAM DETAILS
Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Entrada de aire
industr
INPUT
P-7
P-7
P-3
S-109

Nutrientes

agua

INPUT
P-1

INPUT
P-1

0.00
25.00
1.01
1.18
0.00
0.00
0.24

0.00
40.00
6.01
6.66
793.86
3.63
0.24

0.00
25.00
1.01
1,509.84
0.00
0.00
0.30

0.00
25.00
1.01
994.70
0.00
0.00
1.00

144,265.639
43,796.253
0.000
0.000
188,061.892
159,479,685.725

144,265.639
43,796.253
0.000
0.000
188,061.892
28,224,764.435

0.000
0.000
630.000
0.000
630.000
417.264

0.000
0.000
0.000
20,888.791
20,888.791
21,000.000

S-101
P-1
P-6

S-102
P-6
P-4

S-104
P-6
P-5

S-103
P-4
P-2

0.00
25.00
10.12
1,004.74
0.00
0.00
0.98

0.00
25.00
10.12
1,004.74
0.00
0.00
0.98

0.00
25.00
10.12
1,004.74
0.00
0.00
0.98

0.00
25.00
10.12
1,004.74
0.00
0.00
0.98

630.000
20,888.791
21,518.791
21,417.264

63.000
2,088.879
2,151.879
2,141.726

567.000
18,799.912
19,366.912
19,275.537

63.000
2,088.879
2,151.879
2,141.726

Component Flowrates (kg/batch)

Nitrogen
Oxygen
Glucosa
Water
TOTAL (kg/batch)
TOTAL (L/batch)

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Component Flowrates (kg/batch)

Glucosa
Water
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 4 -

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

entrada de aire
inoculo
INPUT
INPUT
P-2
P-2

Preinoculo

Venteo
inoculacin
P-2
OUTPUT

Inoculo
industrial
P-2
P-3

0.00
25.00
1.01
1,050.00
0.00
0.00
1.00

0.00
25.00
1.01
1.18
0.00
0.00
0.24

0.00
25.00
1.01
1.18
1.98
0.07
0.24

0.00
25.00
1.01
995.22
0.00
0.00
1.00

0.010
0.000
0.000
0.000
0.000
0.010
0.010

0.000
0.000
18,032.427
5,474.296
0.000
23,506.723
19,934,100.972

0.000
59.858
18,034.366
5,434.425
0.000
23,528.648
19,938,585.181

20.908
0.000
0.000
0.000
2,111.591
2,132.499
2,142.745

S-106 Salida industrial

P-5
P-3
P-3
OUTPUT

Cosecha
P-3
P-9

medio agotado
P-9
OUTPUT

Component Flowrates (kg/batch)

Biomass
Carb. Dioxide
Nitrogen
Oxygen
Water
TOTAL (kg/batch)
TOTAL (L/batch)

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

0.00
25.00
10.12
1,004.74
0.00
0.00
0.98

0.00
25.00
1.01
1.18
17.85
0.08
0.24

0.00
25.00
1.01
995.22
0.00
0.00
1.00

0.00
25.14
1.01
994.66
3.28
0.14
1.00

0.000
0.000
0.000
0.000
567.000
18,799.912
19,366.912
19,275.537

0.000
538.714
144,285.023
43,438.008
0.000
0.000
188,261.745
159,522,186.264

208.988
0.000
0.000
0.000
0.000
21,115.913
21,324.901
21,427.368

2.090
0.000
0.000
0.000
0.000
19,939.900
19,941.990
20,049.090

Component Flowrates (kg/batch)

Biomass
Carb. Dioxide
Nitrogen
Oxygen
Glucosa
Water
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 5 -

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

biomasa
P-9
P-11

S-117
P-11
P-8

residual
P-8
OUTPUT

0.00
25.14
1.01
1,002.56
0.23
0.14
1.00

0.00
25.00
1.01
994.92
0.00
0.00
1.00

0.00
40.00
1.01
989.47
24.07
14.97
1.00

206.898
0.000
0.000
1,176.013
1,382.911
1,379.381

5.653
120.747
80.498
1,176.013
1,382.911
1,389.974

5.653
120.747
80.498
1,176.013
1,382.911
1,397.627

Component Flowrates (kg/batch)

Biomass
Citric Acid
residuos
Water
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 6 -

4. OVERALL COMPONENT BALANCE (kg/batch)

COMPONENT
Biomass
Carb. Dioxide
Nitrogen
Citric Acid
Oxygen
residuos
Glucosa
Water
TOTAL

INITIAL

INPUT

OUTPUT

FINAL

IN-OUT

0.000
0.000
48.179
0.000
14.626
0.000
0.000
0.000
62.805

0.010
0.000
162,298.066
0.000
49,270.548
0.000
630.000
20,888.791
233,087.415

7.743
598.572
162,319.388
120.747
48,872.433
80.498
0.000
21,115.913
233,115.294

0.000
0.020
26.856
0.000
8.140
0.000
0.000
0.000
35.016

- 7.733
- 598.592
0.000
- 120.747
404.602
- 80.498
630.000
- 227.122
0.090

- Page 7 -

5. EQUIPMENT CONTENTS
V-101
Procedure

Operation

P-1
P-1
P-1
P-1
P-1

START
CHARGE-1 (Charge)
CHARGE-2 (Charge)
AGITATE-1 (Agitation)
TRANSFER-OUT-1 (Transfer Out)

SFR-101
Procedure

Operation

P-2
P-2
P-2

P-2

START
CHARGE-1 (Charge)
TRANSFER-IN-1 (Transfer In)
FERMENT-1 (Batch Stoich.
Fermentation)
TRANSFER-OUT-1 (Transfer Out)

FR-101
Procedure

Operation

P-2

P-3
P-3
P-3
P-3
P-3

START
TRANSFER-IN-1 (Transfer In)
TRANSFER-IN-2 (Transfer In)
FERMENT-1 (Batch Stoich.
Fermentation)
TRANSFER-OUT-1 (Transfer Out)

Time (in h)

Volume (in L)

0.000
0.917
4.167
5.167
7.417

0.000
417.264
21,417.264
21,417.264
0.000

Time (in h)

Volume (in L)

11.417
11.917
12.517

0.000
0.010
2,141.736

300.517

2,142.745

303.767

0.000

Time (in h)

Volume (in L)

304.517
305.767
308.017

0.000
2,142.745
21,418.283

596.017

21,427.368

599.267

0.000

- Page 8 -