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Clinical Hematology
9
Automated Hematology
Cell Counters
Methodology
Principles
This
Instruments
The
Instruments
Automated full
blood counters with
a five-part or more
differential counting
capacity[*]
Performance
Whole blood is aspirated, diluted, and then divided into
two samples. One sample is used to analyze the red
blood cells and platelets while the second sample is used
to analyze the white blood cells and hemoglobin.
Electrical impedance is used to count the white blood
cells, red blood cells, and platelets as they pass through
an aperture. As each cell is drawn through the aperture, a
change in electrical resistance occurs generating a
voltage pulse. The number of pulses during a cycle
corresponds to the number of cells counted.
The amplitude of each pulse is directly proportional to
the cell volume.
Hemoglobin Measurement
Using cyanide free Hb chemistry methods, rapid
RBCs lysis followed by the formation of an
imidazole-hemoglobin complex with an absorption
peak at 540 nm.
The Cell-Dyn uses electronic sizing to determine a
three part automated differential. The percentage
and absolute counts are determined for
lymphocytes, neutrophil, and mid-size population of
monocytes, basophils, eosinophils, blasts, and other
immature cells.
Results will be used to monitor patients cell counts
and absolute neutrophil count and to determine if
further chemotherapy should be administered.
Specimen Requirements
Whole
Overview
of
Analysis
Modes
Pre-diluted mode
This mode is used in analyzing a minute amount of
childs blood, for instance, collected from the earlobe
or fingertip. In this mode, blood sample diluted into
1:26 before analysis is used. The sample aspiration
procedure is the same as in the whole blood mode.
Note:
Sources Of Errors
Fragmented
Platelet
Giant
platelets:
These are platelets that approach or exceed the
size of the red cells. They cause the right hand
tail of the histogram to remain elevated and
may be seen at the left of the red cell
histogram.
Nucleated
Basic
calculation
Sources
Of Errors In Hematocrit:
Red
Cells Histogram
Plts
Histogram
(0-2)
Air Babbles
Dust
Electronic and Electricalnoise
Over 20 fL
Microcyte
Scishtocyte
WBC's fragments
Giant Plts
Clumped plts
Region
R Flag
Far left(<35fL)
R1
Blasts
Basophilia
Eosinophilia
Plasma cells
Abnormal/variant lymphs
R2
R3
Far right(>450fL)
R4
Multiple flags
RM
Normal Values
Reporting Results
Normal Range
Parameter
4.8-10.8 x 103/L
1. WBC
1. RBC
27-31 pg
1. MCH
32-36 g/dl or %
1. MCHC
11.5-14.5%
1. RDW
150,000 - 450,000/L
1. Platelets
7.4-10.4 fl
1. MPV
1. Hemoglobin
1. Hematocrit
1. MCV
Critical Values
Parameter
Critical Value
WBC (K/mm3)
1.0 or 30.0
HGB (g/dL)
6.5 or 19.0
HCT (%)
20.0 or 60.0
PLT (K/mm3)
30.0 or 1000
Linearity
Parameter
1. WBC (K/L)
Manufacturers Linear
Range
1.0 99.9
1. RBC (M/L)
1.0 7.00
1. HGB (g/dL)
2.5 24.0
1. MCV (fL)
50 200
1. PLT (K/L)
10 999
1. MPV (fL)
5.0 20.0
RBC
WBC
Hgb
1.
2.
3.
4.
MCV
1. Very high WBC
count
2. High concentration
of very large
platelets
3. Agglutinated RBCs
4. RBC fragments
that fall below the
36 fL threshold
5. Rigid RBCs
RDW
MPV
1. Known factors that interfere with the platelet count and shape
of the histogram
2. Known effects of EDTA
Hct
MCH
Known factors that interfere with the parameters used for computation,
Hgb and RBC
MCHC
Known factors that interfere with the parameters used for computation,
Hgb, RBC and MCV
< 40,000
1. Check the integrity of the specimen (look for clots, short draw, etc.)
2. Confirm count with smear review for clumps, RBC fragments,
giant platelets, very small RBCs
WBC
++++
Dilute 1:2 with Isoton or further until count is within linearity (for final result,
multiply diluted result by dilution factor); subtract final WBC from RBC;
perform spun hct, calculate MCV from correct RBC & Hct (MCV = Hct/RBC x
10), do not report HGB, MCH, MCHC. Plt counts are not affected by high WBC.
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