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Y varuna Varuna Biocell Pvt Ltd R&D Unit, Karwat, GT Road, ‘Chandauli 01 ‘wow indizyme.com Performance Test of OzoDet & Safed Ghoda Objective: Comparison of detergent formula 12% sodium salt of sulfonic acid, 12% OzoDet (Biodegradable), Surf Excel Liquid and Safed Ghoda Liquid detergent developed by Varuna (Biodegradable liquid detergent with OzoDet) Samples: . ~ Sample A1 ~ 12% Ozodet All formula containing same excipients only ~ Sample C ~ 12% Acid Slurry Sample |” surfactants are different for comparison - Sample S - Surf Fecal Liquid Sample —» This is Unilever product - Sample SG - Safed Ghoda liquid + —» This is biodegradable liquid detergent detergent developed by Team Varuna Washing conditions: 1. Equipment : DT Apparatus 2. Hardness : 300 ppm 3. Time 15 minutes 4. RPM 2100 5. Concentration : Agmuit 6 Temperature 237°C 7. Measuring Equipment: Laserophot - L2001 8. Standard Stained Fabrics of various codes from EMPA. Results based on whiteness reflectance: Scale: Blackness - 0 and Whiteness - 100. ‘Base Data Sample S | Sample SG EMPA Standard Sample At Sampl im Stained Fabric No. °F Standard “(ozoDet) (Acid Slurry) oe a EMPA 114 195 6.03 50.97 37.83 59.03 EMPA 112, 30.7 37.77 35.6 45.18 40.03 EMPA 167 514 67.33 5763 50.03 55.33 EMPA 116 13.0 28.8 27.43 26.33, 20.56 EMPA 181 675 5187 5087 51.97 51.8 EMPA 185 502 4493 4573 478 52.13 EMPA 414 509 39.00 30.0 72.23 1828 EMPA 164 514 68.68 6773 Bt 70.16 EMPA 104 155 147 1833 41.93 16.86 EMPA 141/1 285 34.23 (31.23 33.76 31.83 EMPA 142/4 199 17.03 8 47.33 19.63 Total Detergency comparison 490.43 460.12 467.49 491.64 Average Detergency comparison 44.58 41.82 42.49 44.69 Result: Safed Ghodais better than Acid Slurry and Surf Excel. OzoDet is beiter surfactant than sulfonic acid salt. Date: April 17, 2014 Performance test of varunia ‘OzoDet & Safed Varuna Biocell Pvt Ltd Ghoda — 17/04/2014 R&D Unit, Karwat, GT Road, Chaneiaull 232101 ‘wanda cam Tested Samples Performance test of varulia OzoDet & Varuna Biocell Pvt Ltd 3 Sated Ghoda — 17/04/2014 R&D Unit, Karwat, GT Road, ey eye Performance test of varuna OzoDet & Safed Varuna Biocell Pvt Ltd 4 Ghoda — 17/04/2014 R&D Unit, Karwat, GT Road, Chandauli 232101 Standard] ‘Wash Result ‘Sample S ‘Sample SG guid) (Sefed Ghoda} Detergen 451.49 mem d91 64 Average Detergency 44.58 41.82 4249 44.69 comparison S

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